Antioxidant activity of aqueous extract of Sonchus arvensis L. in-vitro

Sofnie M. Chairul; Ros Sumarny; Chairul .

doi:10.14499/indonesianjpharm0iss0pp208-215

Antioxidant activity of aqueous extract of Sonchus arvensis L. in-vitro

Sofnie M. Chairul, Ros Sumarny, Chairul .

Abstract

Antioxidants are the compounds which have ability to inhibit reactive oxygen species (ROS) and free radicals in human body. Based on this activitiy the antioxidants prevent cell damage and various pathological processess e.q. cardiovascular, diabetes mellitus, atherosclerosis, respiratory

diseases, carcinogenesis, inflammation, cell degenerative. One of the famous Indonesian traditional medicine is tempuyung or S. arvensis L.(Asteraceae). The antioxidant test has been conducted on the dried water extract by measuring the peroxide value (POV) and in-vitro antioxidant activity test was carried out by spectrophotometry applying thiocyanate method at the wave length of 500 nm.The results showed that peroxide value of dried water extract of tempuyung (S. arvensis L. ) by titration iodometric method has a lower value (865.60) compared to positive control -tocoferol (1142.77). In-vitro antioxidant activity test has remarkable results at the various extract concentrations (1, 5 dan 10 %) and had significant differential absorbance

(A) with positive control (K+) as well as negative control (K-), and among the extract concentrations (P  0,5). Absorbance value increased along with increasing storage time, in which negative control reached higher value of A (0,987) in the seven days and maximal value after eight days (A  1), while the absorbance value of positive control (K+) was 0.648, and dried water extract of tempuyung 1, 5 & 10 % was 0.576, 0.488 & 0.314 after 14 days, respecticly. The absorbance value of absorbance (A) was inversely compared to extract concentrations. Increasing of extract concentrations resulted in decreasing A value, in other words increasing extract concentration resulted in the increas of antioxidant activity.

Key words : Sonchus arvensis L, Asteraceae, POV, antioxidant, thiocyanate method.

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References

Berghe, V.D.A.R., Haemers, A., and Vlientinck, A.J., 1993. in Bioactive Natural Products: Detection, Isolation and Structural Determination; Colegate, S. M., Molyneux, R. J., Eds.; CRC Press: London; Chapter 17, 405-440.

Bors, W., Heller, W., Michel, C., and Saran, M., 1990. in Methods in Enzymology; Packer, L., Glazer, A N., Eds.; Academic Press: New York; 186, 343-355.

Cholbi, MR., Paya, M., and Alcazar, MJ., 1991, Inhibitory effects of phenolic compounds on CCl4-induced microsomal lipid peroxidation, Experient,47,195-199.

Cos, P., Li, Y., Calomme, M., Jia P.H., Cimanga, K., et.al, 1998, Structure-Activity relationship

and classification of flavonoids as inhibitors of xanthin oxidase and superoxide scavengers,

J.Nat.Prod. 61, 71-76.

Farnsworth, N.R. et al. 1985, Medicinal plants in therapy, Bull. World Health Organiz., 63, 965-961.

Haliwell, B., Borish, E. T., Pryor, W. A., et al, 1987, Oxygen of human Disease, Annals. of Internal Medicine,107, 526-545.

Hartono, S., 2002, Kembali ke Obat Tradisional, Majalah Tanaman Obat HERBA, Yayasan Karyasari.

Heyne, K.,1987, Tumbuhan Berguna Indonesia, Jilid II, Yayasan Sarana Wana Jakarta.

Hu, J. P., Calomme, M., Lasure, A., De Bruyne, T., et al. 1995, Biol. Trace Elem. Res. 47, 327-331.

Jamaran, I., 1999, Penerapan teknologi dalam pendayagunaan obat tradisional, Seminar sehari

Pendayagunaan Potensi Obat Tradisional Sebagai Unsur Dalam Pelayanan Kesehatan Masyarakat, BPPT, 9 Maret 1999.

Kikuzaki H., Hara, S., Yayoi, K., and Nakatani, N., 1999, Antioxidative. phenylpropanoids from

berries of Pimenta dioica, Journal of Phytochemistry 52, 1307-1312.

Kosahara. S., and Herni ,S.,1995, Medicinal Herbs Index in Indonesia, Second Edition, PT. Eisai.

Laughton,MJ.,Evans,PJ., Moroney,MA., et al, 1991. Inhibition of mammalian 5-lipoxygenase and cyclo-oxygenase by flavonoids and phenolic and dietary additives. Biochem Phramacol,42,1673-1681.

Masayoshi, I., Ayako, M., Yoshiko, M., Nahoko, T., and Michi, F., 1985, Agric. Biol. Chem. 49, 2173-2176.

Mora,A., Paya, M., Rios, JL., Alcazar MJ. 1990. Structure-activity relationships of polymethoxyflavones and other flavonoids as inhibitors of non-enzymatic lipid peroxidation, Biochem Phamacol, 40,793-797.

Nara, T., Gleye, J., Lavergnede, C.E., and Stanilas, E., 1977, Plant.Med.Phytother. 11, 77.

Pranoto, G., 1999, Potensi dan Strategi Industrialisasi Obat Tradisional Indonesia, dalam Seminar Nasional Pendayagunaan Potensi Obat Tradisional Indonesia sebagai unsur dalam sistem pelayanan kesehatan, BPPT, 9 Maret 1999.

Ramanathan,R., Das,NP., and Tan CH., 1994, Effects of -linolenic acid, flavonoids, and vitamins on cytotoxicity and lipid peroxidation, Free Rad Biol Med.16,43-48.

Robak,J., Greyglewski,RJ.,1988, Flavonoids are scavengers of superoxide anions, Biochem Pharmacol. 37,837-841.

Rumley, A. G., and Paterson, J. R., 1998, Analytical aspects of antioxidants and free radical activity in clinical biochemistry, Annals. of Clinical Biochemistry .35, 181-200.

Salaris, S.C., Babbs, C.F., Voorhees, W. D., III. 1991, Biochem. Pharmacol. 42, 499-506.

Sichel, G., Corsaro, C., Scalia, M., Di Bilio, A. J., and Bonomo, R. P., 1991, Free Rad. Biol. Med 11, 1-8.

Sies, H., 1991. Oxidative Stress : From Basic Research to Clinical Application, Am. Jour. Med. 91 (suppl. C): 3C-31S.

Suwondo, A., 2001, Kebijakan penelitian dan pengembangan kesehatan (Litbangkes) Indonesia, Rapat Kerja Dekan FKM se Indonesia, Jakarta, 27 September 2001.

Wang, P-F., and Zheng R-L., 1992, Inhibition of the autoxidation of linoleic acid by flavonoids in micelles, Chem Phys Lipids. 63,37-40.

Wahyudi, B., 1986, Efek menghambat pembentukan batu kandungan kemih buatan dari infus

tempuyung (Sonchus arvensis L.) pada tikus putih, Medika 12 (10).

Williams, S., 1984, Official Methode of Analysis, 14th edition, Association of Official Analytical

Chemists (AOAC), Airlington, Virginia, 507.

Yuting,C., Rongliang, Z., Zhonghan, J., and Yong ,J., 1990, Flavonoids as superoxide scavengers and antioxidants, Free Rad Biol Med.9,19-21

DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp208-215