ARTEMISININ PRODUCTION BY SHOOT CULTURE OF ARTEMISIA CINA BERG. EX POLJAKOV

Maria Marina; Aziz Purwantoro; C. J. Soegihardjo

doi:10.14499/indonesianjpharm0iss0pp322-325

ARTEMISININ PRODUCTION BY SHOOT CULTURE OF ARTEMISIA CINA BERG. EX POLJAKOV

Maria Marina, Aziz Purwantoro, C. J. Soegihardjo

Abstract

Artemisinin is a secondary metabolite has a potential effect as an antimalaria. Research in artemisinin content in A. cina from shoot culture have not been reported. The aim of this research were:(i) to know the ability of A. cina shoot culture to produce artemisinin, (ii) to asses the effect of concentration of yeast extract, sucrose and their combination in promoting the production of artemisinin, (iii) to determine the highest artemisinin concentration produced by shoot culture in the medium containing different concentration of yeast extract, sucrose and their combination. The factorial of randomised complex block design (RCBD) was used in this experimental design. The first factor was concentration of sucrose ( 1%, 3%, 5%, 7%) and the second factor was concentration of yeast extract (0 mg/l, 100 mg/l, 200 mg/l, 300 mg/l). HPLC was used. To analyse quantitatively the artemisinin production, analysis of variance (ANOVA) and DMRT were used to analyse the data. The results showed that the addition of yeast extract and sucrose increased the concentration of artemisinin from the A. cina shoot culture. The highest artemisinin content produced was 14.035 mg/g dry weights which was produced by combination of 3 % sucrose concentration and 200 mg/l yeast extract. This result suggest that A. cina shoot culture could be used as a method to producing artemisinin.

Key words : Artemisinin, shoot culture, Artemia cina Berg. Ex Poljakov

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References

Aryanti, 2001, Variasi Kandungan Artemisinin dari Akar Rambut dan Regenerasi Artemisia cina Berg ex Poljakov Sebagai Anti Kanker, IPB , Bogor, 12-118.

Brown, G.D., 1992, Two New Compounds from Artemisia annua, J. Nat. Prod., 55(12) : 1756-1760.

Brown, G.D., 1993, Annulide, A Sesquiterpene Lactone from Artemisia annua, Phytochemistry, 32(2) : 391-393.

Ferreira J.F.S dan Janick J., 1996, Distribution of Artemisia annua, Progress in New Crops, 579 – 584.

Ferreira J.F.S., 1996, Root as Enhancing Factor of The Production of Artemisininin Shoot Culture of Artemisia annua, Plant Cell, Tissue and Organ Culture 44 : 211 – 217.

Hartanto, Y., 1997, Kandungan Antrakinon pada Kultur Kalus Anak Daun Ketepeng (Cassia alata L.) dengan Perlakuan Tirosina, Ekstrak Khamir dan Sukrosa, Tesis, Program Pasca Sarjana UGM, Yogyakarta.

Kaul B. and Staba J.E., 1968, Discorea Tissue Culture : I.Biosynthesis and Isolation of Diosgenin from Dioscorea deltoidea Callus and Suspension Cells, Lloydia, 31 : 171 – 179.

Klayman D, 1985, Qinghaosu (Artemisinin) : An Antimalarial Drug from China, Science 228: 1049 – 1055.

Morris P., Scragg A.H., Smart N.J., Stafford A., 1985, Secondary Product Formation by Cell Suspension, in : Plant cell culture – a practical approach (R.A. Dixon, ed), 127-168, IRL Press, Oxford.

Mustofa, 2001, Anti Malaria Klorokuin dan Tinjauan Mekanisme Aksi, Mekanisme Resistensi dan Hubungan Struktur Aktivitas, Seminar Sehari Purna Tugas Prof. Drs. Sardjoko, Apt., Lab. Farmakologi dan Toksikologi Pusat Kedokteran Tropis, Fak. Kedokteran UGM , Yogyakarta.

Paniego dan Giullieth., 1994, Artemisia annua L : Dedifferentiated and Differentiated Cultures, Plant Cell, Tissue and Organ Culture 36 : 163 – 168

Pras N, Visser J F, Batterman S, Woerdenbag H J dan Malingre T, 1991, Laboratory Selection of Artemisia annua L. for High Artemisinin Yielding Types, Phytochemical An, 2, 80 – 83.

Soegihardjo, 1993, Teknologi Kultur Jaringan Tanaman, PAU Bioteknologi UGM Yogyakarta.

Woerdenberg H. J, luers, Uden W., Pras N., Malingre T. M., dan Alferman., 1993, Production of The New Antimalarial Drug Artemisinin in Shoot Culture of Artemisia annua, Plant Cell, Tissue and Organ Culture 32 : 169-174

DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp322-325