Akmal Djamaan, Mohammed Isa Abdul Majid, Mohd. Azizan Mohd. Noor


A fed-batch fermentation with a purpose to increase the production of biodegradable plastic poly(3-hydroxybutyrate), P(3HB), from oleic acid by Erwinia sp. USMI-20 has been carried out. Fermentation was conducted in a conical flask in an incubator rotary shaker at temperature of 30 oC, agitation of 200 rpm for 66 hours. At first stage of fermentation process in a mineral medium, 5 ml of oleic acid as the sole carbon source was used while feeding were done with 2.5 ml of oleic acid at 30, 42 and 54 hours of cultivation. Sampels were collected every 4 hours until 18 hours of cultivation and every 6 hours until 66 hours of cultivation. P(3HB) production was characterized based on the increase of dry cells, polymer content, polymer concentration and the amount of oleic acid and ammonium remaining in the culture. Results showed that by using fed-batch technique Erwinia sp. USMI-20 could produce P(3HB) with a maximum polymer content of 52 % of the dry cell weight, the concentration of polymer of 2.8 g/l, a dry cell weight of 5.4 g/l.

Keywords : Fed-batch, biodegradable plastic, poly(3-hydroxybutyrate, oleic acid).

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Abe, H., Doi, Y., and Yamamoto, Y., 1992, Controlled release of lasted an anti cancer drug from poly(3-hydroxybutyrate) microsphares containing acylglycerols. Macromolecules. Rev. A29: 229-235.

Akmal, D., M.I.A. Majid, and M.N. Azizan, 1998a, Biosynthesis of a copolymer poly(3-hydroxybutyrateco-3-hydroxyvalerate) from palm oil and n-propanol or propionic acid as a second carbon source by Erwinia sp.USMI-20, Program and Abstracts of The International Symposium on Biological Polyhydroxyalkanoates, 9-11 September 1998, Tokyo, JAPAN

Akmal, D., M.I.A. Majid, A. Agustien, L.L. Few, M.S. Razip, M.N. Nazalan and M.N. Azizan, 1998b, Production of a copolymer poly(3-hydroxybutyrate-co- 3-hydroxyvalerate) from palm oil and valeric acid by Erwinia sp.USMI-20, Proceeding of The IMT-GT Uninet Conference, 29-30 August, 1998, Hat Yai, THAILAND, 104-106.

Akmal, D., 2001, Microbial synthesis and characterization of poly(3-hydroxybutyric acid) produced by Erwinia sp. USMI-20 from oleic acid, Jurnal Sains dan Teknologi Farmasi, 6(1), 1-8

Asada, Y., 1996, Plastics from the sun and CO2. Look Japan. July ed.: 26-27.

Brandl, H., Bachofen, R., Mayer, J. and Wintermantel, E., 1995, Degradation and application of polyhydroxyalkanoates. Can. J. Microbiol. 41(Suppl.1): 143-153.

Doi, Y. Kunioka, M. Nakamura, Y. and Soga, K., 1987. Biosynthesis of copolyesters in Alcaligenes autrophus H16 from 13C-labelled acetate and propionate. Macromolecules. 20: 2988-2991.

Doi, Y., 1990, Microbial Polyester, UCH Publ. Inc., New York, 63-86.

Holland, S. J., Tighe, B. J. and Could, P. L., 1987, Polymers for biodegradable medical revices, II: Hydroxybutyrate-hydroxyvalerate copolymers: hydrolytic degradation studies. Biomaterials. 8: 289- 295.

Majid, M. I. A., Hori, K., Akiyama, M. and Doi, Y., 1994, Production of poly(hydroxybutyrate) from plant oil by Alcaligenes sp., in: Biodegradable Plastics and Polymers (Eds. Doi, Y. and Fukuda, K.), Elsevier Science B.V, Amsterdam, 417-424.

Majid M.I.A., D. Akmal, L.L. Few, A. Agustien, M S Toh, M.R. Samian, N. Najimudin and M.N. Azizan, 1999, Production of poly(3-hydroxybutyrate) and its copolymer poly(3-hydroxy-butyrate-co-3-hydroxyvalerate) by Erwinia sp. USMI-20, Int. J. Biol. Macromol., 25: 95-104.

Miller, N. D. and Williams, D. F., 1987, On the biodegradations of poly(b-hydroxybutyrate-hydroxyvalerate copolymers. Biomaterials, 8: 129-137.

Miyake, M., Erata, M. and Asada, Y., 1996, A thermophilic cyanobacterium, Synechococcus sp. MA19, capable of accumulating poly-b-hydroxybutyrate. J. Ferment. Bioeng. 82: 512-514.

Müller, H. M. and Sebach, D. 1993. Poly(hydroxyalkanoates): A fifth class of physiologically important organic biopolymers. Angew. Chem. Int. Ed. Engl. 32: 477-502.

Poirier, Y., Nawrath, C. and Somerville, C., 1995, Production of poly-hydroxyalkanoates, a family of biodegradable plastics and elastromers, in bacteria and plants. Biotechnol. 13:142-150.

Shi, H., Shiraishi, M. and Shimizu, K., 1997, Metabolic flux analysis for biosynthesis of poly(b-hydroxybutyric acid) in Alcaligenes eutrophus from various carbon sources. J. Ferment. Bioeng. 84: 579-587.

Stanbury. P. F. , Whitaker, A. and Hall, S. J., 1995, Principles of Fermentation Technology, 2nd. ed., Pergamon Ltd , Elsevier Science, United Kingdom.

Yamane, T., 1993, Yield of Poli-D-B-hydroxybutyrate from Various Carbon Sources, A Theoritical Study, Biotechnol. Bioeng. 165-170.

DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp256-264


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