BIOTRANSFORMATION OF CINCHONA ALKALOID COMPOUNDS BY MOLD Xylaria sp.TO CINCHONA N-OXIDE ALKALOIDS [Studies on Endophytic Microbes of Cinchona spp. plants (5)]

Partomuan Simanjuntak; Bustanussalam .; Titik K. Prana; K. Ohashi; Hirotaka Shibuya

doi:10.14499/indonesianjpharm0iss0pp95-100

BIOTRANSFORMATION OF CINCHONA ALKALOID COMPOUNDS BY MOLD Xylaria sp.TO CINCHONA N-OXIDE ALKALOIDS [Studies on Endophytic Microbes of Cinchona spp. plants (5)]

Partomuan Simanjuntak, Bustanussalam ., Titik K. Prana, K. Ohashi, Hirotaka Shibuya

Abstract

A biotransformation study on alkaloid chinchona compounds (quinine, quinidine, cinchonidine, cinhonine) by endophytic microbe from Cinchona succirubra have been done. The biotransformation for 2 days after preculture yielded cinchona N-oxide compounds (quinine N-oxide, quinidine N-oxide, cinchonidine N-oxide, cinchonine N-oxide) alkaloids and the bioassay of the compounds also showed effectiveness as antimalaria. Purification the biotransfomed compound was conducted by a high performance liquid chromatograph (HPLC) and determination of chemical structure was done based on carbon nuclear magnetic resonance (NMR).

Key words : Biotransformation, endophyte microbe, Xylaria sp., Cinchona succirubra Pav., cinchona alkaloid, quinine, quinine N-oxide

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DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp95-100