Rina Handayani, Sugiyanto ., Nina Salamah


The leaves of Dewa (Gynura procumbens (Lour) Merr), is utilized in traditional medicine as anticancer, had been reported to contain flavonoid derivatives and supposed to have cytotoxic properties. Hence, these particular substances might be useful to be developed as anticancer in the future. Research on ethanol extract of Gynura procumbens (Lour) Merr leaves as anticarcinogen has been carried out, using toxicity assay model to vero, myeloma and lymphocyte cells. This research applied varied concentrations and adriamycin as a positive control. Observation to the cell growth inhibition was carried out for 4 days. The result showed that the dominant substance (the third spot of paper chromatogram) inhibit the growth of vero and myelome cells but lymphocyte cell. The LC50 of this substance to myelome, vero and lymphocyte cells were < 82.8 g/ml, 103.86 g /ml and > 2650 g/ml, respectively, whereas the LC50 of adriamycin to the cell lines were < 0.015 g /ml. Therefore, substances obtained from Gynura procumbens (Lour) Merr leaves might be developed against cancer.

Key word: Gynura procumbens (Lour) Merr, mieloma


Ames, B.N., Durston, W.C., Yamasaki, E.and Lee, F.D.,1973a. Carcinogens are mutagens: A Simple Test System Combining Liver Homogenates for Activation and Bacteria for Detection, Proc. Natl. Acad. Sci. USA, 70, 2281

Ames, B.N., Lee, F.D. and Durston, W.C. 1973b. An Improved Bacterial Test System for Detection of Mutagens and Carcinogens, Proc.Natl. Acad. Sci. USA, 70, 782

Ames, B.N., Mc Cann, J. and Yamasaki, E., 1975. Methods for Detecting Carcinogens and Mutagens with the Salmonella/Mammalian-microsome Mutagenicity Test, Mutat. Res., 31, 347

Anonim, 1988. Profil Kesehatan Indonesia, Departemen Kesehatan Republik Indonesia, Pusat data Kesehatan, Jakarta, hal. 14, 36

Asepgana Suganda, Iwang Sudiro, Gnathina, 1988. Skrining Fitokimia dan Asam Fenolat Daun Dewa (Gynura procumbens (Lour) Merr), Simposium Penelitian Tumbuhan Obat III, Universitas Indonesia, Jakarta

Athar, M., Khan, W.A. and Mukhtar, H., 1989. Effect of Dietary Tannic Acid on Epidermal, Lung and Forestomach Polycylic Aromatic Hydrocarbon Metabolism and Tumorigenicity in Sencar mice, Cancer Res., 49, 5784

Berenblum, I. and Shubik, P., 1947. The Role of Croton Oil Application Associated with a Single Painting of Carcinogen in Tumor Induction of the Mouse Skin, Br. J. Cancer, 1, 379

Chang, R.L., Levin, W., Wood, A.W., Shirai, N., Ryan, A.J., Duke C.C., Jerina,D.M., Holder, G.M. and conney, A.H., 1986. High tumorrigenicity of the 3,4-dihydrodiol of 7-Metilbenz Acridine on Mouse Skin and Newborn Mice, Cancer Res., 46, 4552

Chu, E.H.Y., 1971. Induction and Analysis of Gene Mutations in Mammalian Cell Cultures, in Hollaender, A. (editor): Chemical Mutagens: Priciples and Methods for Their Detection, Plenum Press, New York, p. 411

Della Porta, G. and Teracini, B., 1969. Chemical carcinogenesis in infant animals, Prog. Exp. Tumor Res., 11, 334

Gelboin, H.V., 1980. Benzo(a)pyrene Metabolism, Activation, and Carcinogenesis: Role and Regulation of Mixed Function Oxidases and Related Enzymes, Physiological Reviews, 60, 4, 1107

Sayer, J.M., Whalen, D.L. and Jerina, D.M., 1989. Chemical Strategies for the Inactivation of Bay-region Diolepoxides, Ultimate Carcinogens Derived from Polycyclic Aromatic Hydrocarbons, Drug Metabol. Rev., 20, 155

Selkirk, J.K., 1980. Chemical Carcinogensesis: A Brief Overview of the Mechanism of Action of Polycyclic Hydrocarbons, Aromatic Amines, Nitrosamines and Aflatoxins, in T.J. Slaga (editor): Carcinogenesis, vol 5, Modifiers of Chemical Carcinogenesis, Raven Press, pp. 1-31

Shimada, T., Martin, M.V., Pruess-Schwartz, D., Marnett, L.J. and Guengerich, F.P., 1989. Roles of Individual Human Cytochrome P-450 Enzymes in the Bioactivation of Benzo(a)pyrene, 7,8-Dihydroxy-7,8-dihydrobenzo(a) Pyrene, and other Dihydrodiol Derivatives of Polycyclic Aromatic Hydrocarbons, Cancer Res., 49, 6304

Smith, J. & Williams, H., 1983. Introduction to The Priciples of Drug Design, Wright P.S.G., Bristol,pp. 165-192

Sudarto, B. & Suwijio Pramono, 1985. Skrining Fitokimia Daun Dewa (Gynura procumbens (Lour) Merr yang Diduga Berkhasiat Sebagai Antikanker, PPPT-UGM, Lembaga Penelitian UGM, Yogyakarta

Sugiyanto, B. Sudarto dan Edi Meiyanto, 1993, Efek Penghambatan Karsinogenitas Benzo (a) Piren oleh Preparat Tradisional Tanaman Gynura sp dan Identifikasi Awal Senyawa yang Berkhasiat, Laporan Penelitian, P4M Ditjen Dikti, Fak Framasi UGM

Sugiyanto, B. Sudarto, Edy Meiyanto dan Sudibyo Marono, 1999, Aktivitas Antikarsinogenik Senyawa yang Berasal dari Tumbuhan, Laporan Komprehensif Penelitian Hibah Bersaing V/3. DP4M, Ditjen Dikti, Fak Farmasi UGM

Thakker, D.R., Yagi, H., Levin, W., Wood, A.W., Coney, A.H. and Jerina, D.M., 1985. Polycyclic Aromatic Hydrocarbons: Metabolic Activation to Ultimate Carcinogens, in M.W. Anders (Editor): Biocativation of Foreign Compounds, Academic Press Inc, New York, pp 177-242

Toth, B., 1968. A Critical Review of Experiments in Chemical Carcinogenesis Using Newborn Animals, Cancer Res., 28, 727

Wattenberg, L.W., Coccia, J.B. and Lam, L.K., 1980 Imhibitory Effects of Phenolic Compounds on Benzo(a)pyrene Induced Neoplasia, Cancer Res., 40,2820

Wattenberg, L.W., 1983. Inhibition of Neoplasia by Minor dietary Constituents, Cancer Res. (Suppl), 43, 2448

Wislocki, P.G. and Lu, A.Y.H., 1988. Carcinogenicity and Mutagenicity of Proximate and Ultimate Carcinogens of Polycyclic Hydrocarbons, in Yang, S.K. and Silverman, B.D. (editors): Polycyclic Aromatic Hydrocarbons Carcinogenesis: Structure-Activity Relationships, Vol I, CRC Press, Boca Rota, Florida, pp 1-30

Wogan, G.N. & Busby, JR, W.F., 1980 Naturally Occuring Carcinogens, dalam I.E. Leiner (editor) Toxic Constituents of Plant Foodstuffs, 2nd edition, Academic Press, pp. 329-360



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