CHROMOSOME CHARACTERIZATION OF THREE VARIETIES OF  GINGER (Zingiber officinaleRosc.)

Budi Setiadi Daryono; Siti Nur Azizah Fauziati Rahma; Purnomo .; Sudarsono .

doi:10.14499/indonesianjpharm23iss1pp54-59

CHROMOSOME CHARACTERIZATION OF THREE VARIETIES OF GINGER (Zingiber officinaleRosc.)

Budi Setiadi Daryono, Siti Nur Azizah Fauziati Rahma, Purnomo ., Sudarsono .

Abstract

Giant ginger (Zingiber officinale Rosc. var. officinale), red ginger (Zingiber officinale Rosc. var. rubra) and small ginger (Zingiber officinale Rosc. var. amarum) are three varieties of Zingiber officinale Rosc. They have a lot of benefit and often used by Indonesian as a traditional drug. Moreover, they have a big chance to be use as a flavor in world wide. Therefore, research for their quality, quantity and continuity of supplyare needed. Characterization of their chromosomes is one effort for improving ginger cultivation. The objective of this research was to study mitotic time and chromosome characters of three varieties of ginger. Squashing method was used for chromosome preparation. The results showed that mitotic time of giant ginger is 09.00-10.05 am, red ginger is 09.00-10.30 am, while small ginger is 08.45-11.00 am. Chromosome number of giant ginger and small ginger are 2n=2x=30, while red ginger is 2n=2x=22. Giant ginger has R= 3,109, Red ginger has R = 3,206 and small ginger has R = 4,065. Based on chromosome characters it is revealed that relationship between giant ginger and red ginger is closer that of compare to small ginger. This result is important as basic information for improving the gingers production through breeding program.

Key words: Zingiber officinale Rosc., mitotic time, chromosome characterization, squashmethod

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References

Ali, B.H., Blunden G., Tanira, M.O. and Nemmar A., 2008, Some phytocemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): A review of recent research, food and Chemical Toxicology46: 409-420

Chakravorti, A.K.,1948, Multiplication of chromosome numbers in relation to speciation in Zingiberaceae. Sci. & Cult. 14:137–140

Darlington, C.D., and Janaki Ammal, E.K., 1945, Chromosome Atlas of Cultivated Plants, George Allen & Unwin, London, p. 397

Eksomtramage, L., Sirirugsa, P., Jivanit, P., and Maknoi, C., 2002, Chromosome Counts of Some Zingiberaceous Species from Thailand, Songklanakarin Journal of Science and Technology24 (2):311-319

Etikawati, N. dan Setyawan, A. D., 2000, Studi Sitotaksonomi pada Genus Zingiber, Jurnal Biodiversitas 1 (1): 8-13

Ghayur, M.N. and Gilani, A.H, 2005, Ginger Lowers Blood Pressure Through Blockade of Voltage-Dependent Calcium Channels, J. Cardiovascular Pharmacology45 (1): 1-7

Hamiudin, 2007, Budidaya Jahe (Zingiber officinale),(Online), 1-16, (http: www.skma_org. com, Accessed on September 6, 2008).

Heba, A.A., Windeck, T., Ploch, M., and Vesophl, E.J., 2006, Mode of Action of Gingerols and Shogaols on 5-HT3receptors : Binding Studies, Cation uptake by the Receptor Channel and Contraction of Isolated Guinea-Pig Ileum, Eur J Pharmacol530:136-143

Jahier, J. and Tanguy, A.M., 1996, Observation of Chromosomes: Basic Techniques (Mitosis) of Plant Cytogenetics, Science Publisher Inc.,USA, pp. 3-5

Jolad, S.D., Lantz, R.C., Solyon, A.M., Chen, G.J., Bates, R.B., Timmermann, B.N., 2004, Fresh Organically grown ginger (Zingiber officinale) : Composition and Effects on LPS-induced PGE2 production, Phytochemistry65:1937-1954

Judd, W.S., Campbell C.S., Kellogg E. A. and Stevens, P.F., 1999, Plant Systematics A Phylogenetic Approach, Sinauer Associates Inc., USA

Moringa, T., Fukushina, E., Kanui, T., and Tamasaki, Y., 1929, Chromosome numbers of cultivatedplants. Bot. Mag. (Tokyo) 43:589–594

Raghavan, T.S., and Venkatasubban, K.R., 1943, Cytological studies in the family Zingiberaceae with special reference to chromosome number and cytotaxonomy. Proc. Indian Acad. Sci. 17B:118–132

Ramachandran, K., 1969, Chromosome numbers in Zingiberaceae. Cytologia, 34:213-221

Ratnambal, M.J., 1979, Cytological studies in ginger (Zingiber officinale Rosc.). Unpublished Ph.D thesis, University of Bombay, India.

Ravindran, P. N. and Babu, K.N., 2008, Ginger: The Genus Zingiber, CRC Press, New York, pp.39-43

Rehman, R., Akram, M., Akhtar, N., Jabeen, Q.,Saeed, T., Shah, S.M.A., Ahmed, K., Shaheen, G., and Asif, H.M., 2011,

Zingiber officinaleRoscoe (Pharmacological activity), Journal of Medicinal Plants Research 5 (3): 344-348

Saensouk, S. and Saensuk, P., 2004, Chromosome Numbers of Zingiberaceae in Thailand, KKU Res Journal 9 (1):3-9

Sanpote, P., 2004, Species Diversity and Chromosome Number of Some Zingiberaceae in the Region of Thailand,

Thailand J. of Genetics(Online), vol. 20, no 3 (http://www.scielo.th/scielo.php?script=sci_arte xt&pid-S0100-84551997000300021, accessed on March 13, 2008)

Singh, A., Duggal, S., Singh, J and Katekhaye, S., 2010, Experimental Advances InPharmacology of Gingerol and Analgues, Pharmacy Globale (Int. J. Of Comprhensive Pharmacy) (Online), vol.1,issue 2, pp. 1-5 (http:www.pharmacie-globale.info, accessed on October 19, 2011)

Singh, G., 1999, Plant Systematic, Science Publisher Inc.,USA, pp. 78, 176-180

Sharma, A.K., and Bhattacharya, N.K. ,1959, Cytology of several members of Zingiberaceae and a study of the inconsistency of their chromosome complement, La Cellule59:279–349

Sudarnadi, H, 1989, Taksonomi Tumbuhan Berpembuluh I, Departemen Pendidikan dan Kebudayaan Direktorat Jenderal Pendidikan Tinggi, Bogor, pp. 42-44

Suekawa, M., Ishige, A., Yuasa, K., Sudo, K., Aburada, M., and Hosoya, E., 1984, Pharmacological Studies on Ginger. I.Pharmacological actions of pungent constituents, (6)-gingerol and (6)-shogaol, J.Pharmacobiodynamics, 7:13-18

Sugiura, T., 1936, Studies on the chromosome numbers in higher plants, Cytologia 7:544–595

Srivastava, K.C. and Mustafa, T., 1989, Ginger (Zingiber officinale) and rheumatic disorders, Med., Hypoth.29:25-28

Tamarin, R. H., 1999, Principle of Genetics 6th edition, Mc Graw-Hill Companies, Boston, p. 49

DOI: http://dx.doi.org/10.14499/indonesianjpharm23iss1pp54-59