Efek flavonoid daun srikaya (Annona squamosa (L)) terhadap pemacuan ekspresi P53 sel HeLa

Laela Hayu Nurani, Suwidjiyo Pramono, Sudjadi .


Flavonoids  are  secondary  metabolites  with  many  activities  such  as  an anticancer  and  an  antioxidant. Annona  squamosa L.  leave  are  reported  contain flavonoids. Annona squamosa flavonoids were isolated from ether fraction and separated by paper chromatography with acetic acid 15% as mobile phase. Four flavonoids   with  Rf  value  of  0.11,  0.23,  0.46  and  0.78  were  detected  as  5-hydroxy-7-R-flavonol  (1),  5,3’,4’-trihydroxy-7-R-flavonol  (2),  5-hydroxy-7-Rflavanone  (3)  and  7-R-flavanone  (4),  respectively.  The  cytotoxicity  of  each flavonoid  on  Hella  cell  line  was  carried   out   using  direct  counting  method  and the p53 expression was done with immunocytochemistry. The result showed that LC50  of  flavonoid 1, 2, 3 and  4 was  12.63±3.4,  18.47±3.4,  75.74±6.2  and 204.4±14.0  µg/mL.   Twelve   µg/mL  of  5-hydroxy-7-R-flavonol  (1)   increased p53 expression of eleven fold than control.

Keywords: Annona squamosa, HeLa, flavonoid, p53


Full Text:



Amslinger, 2010, The Tunable Functionality of α,β-Unsaturated Carbonyl Compounds Enables Their Differential Application in Biological Systems, ChemMedChem, 5(3)351-356

Baege, A.C., Berger, A., Schlegel, R., and Veldman, T., 2002, Cervical Epithelial Cells Transduced with the Papillomavirus E6/E7 Oncogenes Maintain Stable Levels of Oncoprotein Expression but Exhibit Progressive, Major Increases in hTERT Gene Expression and Telomerase Activity, Am J. Pathol, 160(4),1251-1257

Biva, I.J., Vhuiyan, M.M.I., Saha, M.R., Islam, M.S., and Shammy Sarwar, S., 2009, In-vitro Antioxidant and Cytotoxicity Studies of Annona squamosa Linn, S.J. Pharm. Sci., 2(1) 32-36

Cos, P., Calomme, M, Pieters, L, Vlietinck, A.J., and Berghe, V.D., 2000, Structure-activity relationship of flavonoids as antioxidant and pro-oxidant compounds, Studies Natural Products Chemistry, 22(3)307-341

Khan, N., Afaq, F., , Syed, D.N. and Mukhtar, H. 2008, Fisetin. a novel dietary flavonoid, causes apoptosis and cellcycle arrest in human prostate cancer LNCaP cells. Carcinogenesis 29(5):1049-1056.

Kazua, H., Satoko, A., Reiko, Y., Uno, N., and Yoshinori, F., 1993, Structural studies of polyhidroxybis acetogenins from Annona squamosaL. using combination of chemical derivatisazion and precursor ion scanning mass spectrophotometry, Org. Mass. Spectrom, 28(12)L 1516-1524

Intaranongpai, J., Chavasiri, W., and Gritsanapan, W., 2006, Anti-head lice effect ofAnnona squamosaseeds, Southeast Asian J Trop Med Public Health, 37(3): 532-535

Li, J., Y Cheng, Y., W Qu, W., Y Sun, Y., Z Wang, Z., H Wang, H., B. and Tian, B., 2010, Fisetin, a Dietary Flavonoid, Induces Cell Cycle Arrest and Apoptosis through Activation of p53 and Inhibition of NF-Kappa B Pathways in Bladder Cancer Cells, Basic Clin Pharmacol Toxicol,

Nishimura, A., Nakahara, T., Ueno, T., Sasaki, K., Yoshida, S., Kyo, S., Howley, P.M., and Sakai, H., 2006, Requirement of E7 oncoprotein for viability of HeLa cells, Microbes and Infection, 8: 984-993

Nurani, L.H., 2004, Isolasi dan identifikasi flavonoid infusa daun Srikaya (Annona squamosa, L) dan uji antiproliferasi terhadap sel HeLa, Thesis, Fakultas Farmasi UGM, Yogyakarta

Panda S., and Kar, A., 2007, Antidiabetic and antioxidative effects of Annona squamosaleaves are possibly mediated through quercetin-3-O-glucoside, BioFactors, 31:201-210

Priyadarsini, RV., Murugan,RS., Maitreyi, S., Ramalingam, K., Karunagaran, D., and Nagini, S., 2010, The flavonoid quercetin induces cell cycle arrest and mitochondria-mediated apoptosis in human cervical cancer (HeLa) cells through p53 induction and NF-κB inhibition, Eur J Pharmacol, 649(1-3):84-91.

Shirwaikar A, Rajendran K, and Kumar CD., 2004, In vitro antioxidant studies of Annona squamosa Linn. Leaves, Indian J Exp Biol, 42(8):803-7.

Sofyan, R., 2000, Terapi kanker pada tingkat molekuler, Cermin Dunia Kedokteran, 127:5-10

Teissier, S., Pang, C.L., and Thierry, F., 2010, The E2F5 repressor is an activator of E6/E7 transcription and of the S-phase entry in HPV18-associated cells, Oncogene, 29(36):5061-70.

Tjindarbumi,D, and Mangunkusumo,R., 2002, Cancer in Indonesia, Present and Future, Jpn J Clin Oncol; 32 (Supplement 1) ,17-21

Zhang, H.M., Yuan, J., Cheung, P., Chau, D., Wong, B.W., McManus, B.M., and Yang D., 2005, Gamma Interferon-Inducible Protein 10 Induces HeLa Cell Apoptosis through a p53-Dependent Pathway Initiated by Suppression of Human Papillomavirus Type 18 E6 and E7 Expression, Molecular and Cellular Biology, 25(14):6247-6258

DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp273-278


  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian J Pharm indexed by:

analytics View My Stats