Mucous dilution activity of non soluble ethylacetate fraction syrup of hibiscus flowers (Hibiscus rosa-sinensis L.) at cow intestinein vitro

Mimiek Murrukmihadi, Subagus Wahyuono, Marchaban ., Sudibyo Martono


Previous  study  indicated  that  ethylacetate  insoluble  fraction  of  ethanol extract  of  Hibiscus  flowers  (Hibiscus  rosa-sinensis L.)  were  able  to  dilute intestine cattle mucous in vitrothat was often associated with mucolytic activity.In  this  study,  syrup  preparation  of  various  concentrations  of  these  fractions (1.20,  1.60  and  2.00%)  was  tested  for  its  ability  to  dilute  of  mucous.  Each  of syrup  concentrations  was  mixed  with  20%  buffer  Phosphate  (pH,  7)  at  equal

proportion (1:1 v/v), the resulting mixtures were tested for their ability to dilute mucous  in  vitro. Mucous  dilution  was  observed  by  flow  time  recorded in  the viscosimeter.  The  study  showed  that  all  syrup  concentrations  (1.20,  1.60, 2.00%) exhibited mucous dilution effect compared tonegative control and syrup concentration  at  2.00%  was  comparable  to  positive  control  acetylsistein  0.1% (p<0.05). Therefore, there was a possibility that thispreparation could be used as mucolytic.

Keywords: Hibiscus rosa-sinensisL., viscosity, mucous dilution, mucolytic

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Anonim, 1985, Tanaman Obat Indonesia, Jilid Pertama, Departemen Kesehatan Republik Indonesia, Jakarta, 44.

Anonim, 1991, Penapisan Farmakologi, Pengujian Fitokimia dan Pengujian Klinik, Kelompok Kerja Ilmiah Yayasan Pengembangan Obat Bahan Alam Phyto Medica, Jakarta, 69-71.

Gauthaman, K.K., Saleem, M.T.S., Thanislas, P.T., Prabhu, V.V., Krishnamoorthy, K.K., Devaraj, N.S., and Somasundaram, J.S., 2006, Cardioprotective Effect of the Hibiscus rosa sinensis Flowers in An Oxidative Stress Model of Myocardial Ischemic Reperfusion Injury in Rat, BMC Complementary and Alternative Medicine, 6, 32-39.

Murrukmihadi, M., Wahyuono, S., Marchaban, and Martono, S., 2010, Isolasi dan Penetapan Kadar Alkaloid Ekstrak Etanol Bunga Kembang Sepatu (Hibiscus rosa-sinensis L.), Jifi,September, 9,2.

Murrukmihadi, M., Wahyuono, S., Marchaban, and Martono, S., 2011, Optimasi Formulasi Sirup Fraksi Tidak Larut Etilasetat yang Mengandung Alkaloid Dari Bunga Kembang Sepatu (Hibiscus rosa-sinensisL.),MOT, Mei-Agustus,16, 2, 101-108.

Puckhaber, L.S., Stipanovic R.D., and Bost, G.A., 2002, Analyses for Flavonoid Aglycones in Fresh and Preserved Hibiscus Flowers, /v5-556.html, 26 Mei 2008.

Siddiqui, A.A., Wani, S.M., Rajesh, R., and Alagarsamy, V., 2006, Phytochemical and Pharmacological Investigation of Flowers of Hibiscus rosa-sinensisLinn, Indian J. Pharm. Sci., 68(1), 127-130.

Skarayadi, O., Gana, A., dan Yulinah, E., 2004, Uji Aktivitas Antimikroba Ekstrak Etanol Lima Tanaman Obat, Skripsi, Fakultas Farmasi Institut Teknologi Bandung, Bandung.

Tomkiewicz,E.M., Coffiner, M., Fossion, J., Maes, P., and King, M., 1994, Mucolytic Treatment with N-acetylcysteine L-lysinate metered dose inhaler in dogs : airway epithelial function changes, Eur Respir J., 7,81-87.

Vasudeva, N. and Sharma, S.K., 2008, Post-Coital Antifertility Activity of Hibiscus rosa-sinensis Linn. Roots, eCAM, 5(1), 91-9.



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