Antihyperurisemia  activity  of  water  extract  of  black  seed  (Coleus ambonicusLour) in balb-c mice and its standardization

Andi Suhendi; Nurcahyanti .; Muhtadi .; EM Sutrisna

doi:10.14499/indonesianjpharm0iss0pp77-84

Antihyperurisemia activity of water extract of black seed (Coleus ambonicusLour) in balb-c mice and its standardization

Andi Suhendi, Nurcahyanti ., Muhtadi ., EM Sutrisna

Abstract

Effect of water extract of black seed on uric acid metabolism in hyperuricemia mice models were studied. The influence of water extract of black seed on uric acid biosynthesis was xamined using mice treated by 250 mg/ body weight potassium oxonate intra peritoneal. The result showed that water extract of black seed (200 mg/kg body weight, per oral) have a significant effect in reduction in plasma uric acid levels in comparison to negative control, but less active than positive control (allopurinol). The levels of uric acid in mice plasma for negative control, black seed extract and positive control were 3.100 ± 0.346 mg/dL, 0.200 ± 0.100 mg/dL dan 1.200 ± 0.561 mg/dL, respectively. The standardization performed by determined specific and non specific parameters based on National Bureau of Food and Drug, Republic of Indonesia. Specific parameters of water extract of black seed are phenolic content, and flavonoid content are 0.664 ± 0.035 % and 0.400 ± 0.018 %, respectively. Non specific parameters of water extract of black seed are loss of weight, water content, ash content, ash insoluble acid content, heavy metals contamination (Pb and Cd), and aflatoxin residue are 14.93-14.99%, 6.09-7.89%, 7.12-7.49%, 5.20-6.66%, 6.405 +0.211 ppm, 0.0096 +0, not detected, respectively.

Key words: antihyperuricemia effect, water extract of black seed, standardization

Full Text:

Untitled

References

Ghafar, M, F A., Prasad, K, N., Weng, K, K., and Ismail, A., 2010, Flavonoid, hesperidine, total phenolic contents and antioxidant activities from Citrus species, African Journal of Biotechnology,9(3), pp. 326-330

Abdulelah, H.A.A. and Zainal-Abidin, B. A. H., 2007, In Vivo Anti-malarial Tests of Nigella sativa (Black Seed) Different Extracts, American J.of Pharmacology and Toxicology2 (2): 46-50

Ariyanti, R., 2007, Pengaruh Pemberian Infusa DaunSalam (Eugenia polyantha Wight.)Terhadap Penurunan Kadar Asam Urat dalam Darah Mencit Putih Jantan hiperurisemia, Skripsi, Fakultas Farmasi Universitas Muhammadiyah Surakarta, Surakarta.

Dawiesah, I. S., 1989, Penentuan Nutrien dalam Jaringan dan Plasma Tubuh, Hal 54-61, PAU Pangan dan Gizi, UGM. Yogyakarta.

El-Sawi, S.A. and Sleem, A, A., 2010, Flavonoids and Hepatoprotective Activity of Leaves of Senna Surattensis (Burm.f.) In CCl4 Induced Hepatotoxicity in Rats, Australian J. of Basic and Applied Sciences, 4(6): 1326-1334

Handadari, H. R., 2007, Efek Decocta Daun Salam (Eugenia polyantha Wight.) Terhadap Penurunan Kadar Asam Urat dalam Darah Mencit Putih (Mus muculus) Jantan hiperurisemia, Skripsi, Fakultas Farmasi Universitas Muhammadiyah Surakarta, Surakarta.

Hokazono, H., Omori, T., and Ono, K., 2010, Anti-hyperuricemic Effect of Fermented Barley Extract is Associated with Increased Urinary Uric Acid Excretion, Food Sci. Technol. Res., 16 (4), 295 – 304

Iswantini, D., Darusman, L, K., and Hidayat, R., 2009, Indonesian Sidaguri (Sida rhombifolia L.) as Antigout and Inhibition Kinetics of Flavonoids Crude Extract on the Activity of Xanthin e oxidase, J. Biological Sciences9 (5):504-508

Jacob, RA., Spinozzi, GM., Simon, VA., Kelley, DS.,Prior, RL., Hess-Pierce, B. and Kader, AA. 2003., Consumption of cherries lowers plasma urate in healthy women., J. Nutr., 133, 1826-1829.

Kanter, M., Coskun, O., and Uysal, H., 2006, The antioxidative and antihistaminic effect of Nigella sativa and its major constituent, thymoquinone on ethanol-induced gastric mucosal damage, Arch Toxicol., 80: 217–224,

Katzung, B. G., dan Trevor, A. J., 1994, Buku Bantu Farmakologi, diterjemahkan oleh Staf Pengajar, Laboratorium Farmakologi, Fakultas Kedokteran dan Universitas Sriwijaya, Cetakan I, Penerbit Buku Kedokteran EGC, Jakarta.

Kozin, F., 1993, Terapi Mutakhir CONN (Conn’s Current Therapy), EGC, Jakarta, 1884-1985.

Mahmudah, T, R., 2010, Efek antihelmintik ekstrak biji jintan hitam (Nigella sativa) TERHADAP Ascaris suum Goeze in vitro, Skripsi, Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Martin, D. W., 1987, Metabolisme Nukleotida Purin dan Pirimidin dalam Biokimia Harper, Edisi 20, diterjemahkan oleh Darmawan, Iyan, Penerbit Buku Kedokteran EGC, Jakarta.

Mohamed, D, A., and Al Okbi, S, Y., 2008, Evaluation of anti-gout activity of some plant food extracts, Pol. J. Food Nutr. Sci. 2008, 58, No. 3, 389-395

Moriwaki, Y., Okuda, C., Yamamoto, A., Ka, T., Tsutsumi Z., Takahashi, S., Yamamoto, T., Kitadate, K and Wakame, K., Effects of Oligonol, an oligomerized polyphenol formulated from lychee fruit, on serum concentration and urinary excretion of uric acid. J. of Functional Food, 10.(1016)

Moussaoui, F., Zellagui, A., Segueni, N., Touil, A., and Rhouati, S., 2010, Flavonoid Constituents from Algerian Launaea resedifolia (O.K.) and Their Antimicrobial Activity, Rec. Nat. Prod. 4:1, 91-95

Mutschler, E., 1991, Dinamika Obat, Buku Ajar Farmakologi dan Toksikologi, Edisi Kelima, ITB, Bandung, 217-221.

Islam, S, K N., Ahsan, M., Hassan, C, M., And Malek, M, A., 1989, Antifungal Activities Of The Oils Of Nigella Sativa Seeds, Pakistan J. Pharm. Sci. 2(1):25 – 28

Schunack, W., Mayer, and K., Manfred, H., 1990, Senyawa Obat Kimia Farmasi, diterjemahkan oleh Joke, Witlmena dan Soebita, S., Gajah Mada University Press, Yogyakarta.

Shamley, D., 2005, Pathophysiology an Essential Text for the Allied Health Professions, Elsevier Limited, USA.

Thippeswamy, N, B., and Naidu, K, A., 2005, Antioxidant potency of cumin varieties—cumin, black cumin and bitter cumin—on antioxidant systems, Eur Food Res Technol (2005) 220:472–476, DOI 10.1007/s00217-004-1087-y

Waterhouse, A. L., 2002, Current Protocols in Food Analytical Chemistry., 11.1.1-11. 1.8

DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp77-84