Inhibitory effects of daun sendok (Plantago major L.) on mast cell-mediated anaphylactic reaction

Agung Endro Nugroho, Erna Prawita Setyowati, Zullies Ikawati


Hypersensitisation reaction, which is known as an allergy, is suffered by a lot of people in the modern society. An increase of allergen uptake and the decrease of immune system are considered to be the major factors, which influence the disease. Development of medicinal plants for the allergic reaction is continuing with the extract of Plantago major as an example. This extract could inhibit histamine release from cell culture of Rat Basophilic Leukaemia (RBL-2H3) induced by DNP-BSA in vitro. This study determined the effect of Plantago major L. on systemic anaphylactic reaction induced by compound 48/80 in vivo.
The research was conducted using Balb/c mice (2-3 months, 20-30 gram) which were divided into 2 main groups i.e. (1) those which were given with etanol extract on systemic anaphylactic reaction, and (2) those which were given with n-hexane extract on systemic anaphylactic reaction. Each group was divided into five sub-groups based on various dosages of Plantago major extract i.e. 0.1, 1.0, 10.0, 100.0, 1000.0 mg/kg BW. Compound 48/80, a histamine release inductor, was administered at dose of 8 mg/kg BW, an hour after Plantago major administration, and the total of death mice were observed for an hour. Pharmacological parameter, ED50 value, was determined using probit analysis.
The result showed that both etanol and n-hexane extracts of Plantago major L could inhibit systemic anaphylactic reaction induced by compound 48/80 with ED50 values of 1.66 and 55.01 mg/kg BW, respectively. It can be concluded that the potency of etanol extract of Plantago major L is higher than its n-hexane extract.
Key words: Plantago major L., anaphylactic, histamine and mast cells

Full Text:



Holgate, S.T., 1999, The epidemic of allergy and asthma, Nature, 402, B2

Ikawati, Z., Wahyuono, S., Maeyama, K., 2001, Screening of several Indonesian medicinal plants for their inhibitory effect on histamine release from RBL-2H3 cells, J Ethnopharmacol 75,249-256

Kawasaki, M., Toyoda, M., Teshima, R., 1994. In vitro antiallergenic acitivity of flavonoids in histamine-release assay using rat basophilic leukemia (RBL-2H3) cells, Journal of the Food Hygienic Society of Japan, 35, 497–503.

Kim, H.M., Lee, E.H., Jeoung, S.W., Kim, C.Y., Park, S. T., Kim. J.J., 1999, Effect of Korean folk medicine “Chung Dae San” on mast cell-dependent anaphylactic reaction, J.Ethnopharm, 64, 45-52

Koibuchi, Y., Ichikawa, A., Nakagawa, M., Tomita, K., 1985, Histamine release induced from mast cells by active components of compound 48/80, Eur J Pharmacol 115, 163-170

Samuelsen, A. B., 2001, The traditional uses, chemical constituents and biological activities of Plantago major L. (A review), Journal of Ethnopharmacology, 71 : 1–21.

Toyoda, M., Tanaka, K., Hoshino, K., Akiyama, H., Tanimura, A., Saito, Y., 1997, Profiles of potentially antiallergic flavonoids in 27 kinds of health tea and green tea infusions, Journal of Agricultural and Food Chemistry, 45 : 2561–2564.



  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian J Pharm indexed by:

analytics View My Stats