Standardization of pegagan extract (Centella asiatica (L.) Urban) based on siaticoside content using TLCdensitometricmethod
Process of standardization was initiated by searching the best solvent for extraction. Water, ethanol 30%, 50%, and 70% were used for maceration of the herb. Asiaticoside contents were then determined by TLC-densitometry.
The result of research showed that ethanol 70 % could dissolve highest concentration of asiaticoside from pegagan herb so it was then used for larger scale extraction of three different plant materials. TLC-densitometric data showed that asiaticoside contents of pegagan herb originally from Kaliurang were 0.98 ± 0.13.%, Boyolali were 1.34 ± 0.20.%, and Tawangmangu were 0.21 ± 0.3 %.
Key words: Centella asiatica (L.) Urban, asiaticoside, TLC-densitometry
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