Effect of curcumin and 4-n-propil curcumin on glutation s-transferase activity in rat liver

Fitriyah Kusumawati, Sudibyo Martono, Supardjan .


Curcumin, a plant phenol in rhizome Curcuma longa L. has been reported to be a potent inhibitor of glutathione S-transferase (GST) in rat liver with in vitro with substrate 1,2-dikloro-4-nitrobenzen (DCNB). By using DCNB, a specific substrate for GST mu, This work was aimed to know whether curcumin derivatives: 4-n-propyl curcumin can also inhibit GST activity in vitro.
Glutathione activity can be measured by conjugating GSH and DCNB catalized with GST. The product can be measured spectrofotometrically. Curcumin and its derivatives decreased GST activities with IC50 values 6,86 μM for curcumin and 8,41 μM for 4-n-propyl curcumin. This means that 4-n-propyl curcumin has a weaker inhibition potency than curcumin.
Curcumin (consentration 10μM) inhibited GST activity by mixed with the Ki dan Ki’ values 2,77 μM dan 7,33 μM, whereas 4-n-propyl curcumin (consentration 10μM) inhibited GST activity by competitive with the Ki values 6,28 μM.
Key words : Curcumin, 4-n-propyl curcumin, glutathione S-transferase, 1,2-dikloro-4-nitrobenzen.

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Bradford, M.M., 1976, A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding, Anal. Biochem., 72,248-254.

Cai, P., Bennet, D., Nair, R.V., Ceska, O., Smith, M.J.A., and Giovanni, J.D., 1993, Inhibition and Inactivation of Murine Hepatic Ethoxy- and Pentoxyresorufin O-Dealkylase by Naturally Occuring Coumarins, Chem. Res. Toxicol., 6, 872-879.

Commandeur, J.N.M., Stijntjes, G., and Vermeulen, N.P.E., 1995, Enzymes and Transport Systems Involved in The Formation and Disposition of Glutathione S-Conjugates, Pharmacol. Rev.,47 (2), 271-330.

Das, M., Bickers, D.R., and Mukhtar, H., 1984, Plant Phenols as In Vitro Inhibitors of Glutathione S-Transferase, Biochem. Biophys. Res. Commun., 120 (2), 427-433.

Habig, W.H., Pabst, M.J., and Jakoby, W.B., 1974, Glutathione S-Transferase, the first enzymatic step in mercapturic acid formation, J. Biol. Chem., 249 (22), 7130-7139.

Mannervik, B. dan Danielson, V.H., 1988, Glutathione S-Transferase Structure and Catalytic Activity, CRC. Crit. Rev. Biochem., 23, 283-337.

Metzler, D.E., 1977, Biochemistry, the Chemical Reaction of Living Cells, Academic Press, Inc., New York, p. 301-318.

Sudibyo, M., 1996, Efek Hambatan Kurkumin dan Analognya pada Aktivitas Glutation STransferase Liver Tikus secara in vitro, Majalah Farmasi Indonesia, 7, 31-51.

Sudibyo, M., 1997, Studi Inhibisi Kurkumin terhadap Aktivitas Enzim Glutation S-Transferase Liver Tikus yang Diinduksi Fenobarbital, Laporan Penelitian DPP-SPP, Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta.

Sudibyo, M., 1999, Uji Penghambatan Aktivitas Glutation S-Transferase Liver Tikus oleh 2,5–bis-(4-hidroksi-3-metoksi benzilidin) siklopentanon dan dua analognya, Lembaga Penelitian, Universitas Gadjah Mada, Yogyakarta.

Sudibyo, M., 2000, Inhibition of glutathione S-transferase by curcumin and its derivatives, Molecular mechanisms and qualitative structure-activity relationships, Dissertation, Gadjah Mada University, Yogyakarta, Indonesia.

Supardjan, A. M., 1998, Synthesis and Anti-inflammatory Activity of Some 4-substitued Curcumin Derivatives, Dissertation, Gadjah Mada University, Yogyakarta.

DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp89-95


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