Isolation and identification artemisinine from product of endophyte microbe cultivation from Artemisia annua. [studies on endophytic microbes of Artemisia spp. (3)]

Partomuan Simanjuntak, Bustanussalam ., Dian Malini Otovina, M. Rahayuningsih, E.G. Said


Isolation of the biotransformation product artemisinine from endophitic bacteria is a new alternative for the production of the multidrugresistant antimalaria compounds. The bacteria strain, AT 12, has been proved particularly potential as an artemisinin source.
This working was carried out to isolate and to identify the biotransformation product artemisinin as an antimalarial active compound from endophitic bacteria strains of Artemisia annua. The IR spectrum exhibited the characteristic bands for δ-lactone (1750-1735 cm-1, 1250-1111 cm-1) and endoperoxide function (~1115 cm-1, 890-830 cm-1). As a result, the GC-MS gave a positively identification for the majority of GC peaks. The mass spectrum of the peak (Rt 27.94 min) has a molecular ion of artemisinin with one hydrogen loss at m/z 281, representing (M+ - 1).
Keywords: Artemisia spp. Artemisia annua, Endophytic, Artemisinine, sesquiterpene

Full Text:



Bicchi C, Rubiolo P. 1996. High-performance liquid chromatographic-particle beam mass spectrometric analysis of sesquiterpene lactones with different carbon skeletons. J. Chromatogr A 727:211-221.

ElSohly, H.N. , Croom E.M., El-Feraly F.S., dan El-Sherey M.M., 1990. A large-scale extraction technique of artemisinin from Artemisia annua. J. Nat. Prod. 53 (6), 1560 – 1564

Geldre EV, Vergauwe A, Eechout E., 1997. State of art of the production of antimalarial compound artemisinine in plants. Plant Mol. Biol. 33 , 199 - 209

Klayman DL. 1993. Artemisia annua: from weed to expectable antimalarial plant. Di dalam: Kinghorn AD, Balandrin MF, editor. Human Medicinal Agent From Plant. ACS Symposium Series 534. Washington DC: American Chemical Soc, 242-250.

Melliawaty, R., Dian Malini Oktovina, T. Parwati dan P. Simanjuntak. 2004. Studi mikroba endofit tanaman Artemisia annua (2) : Produksi senyawa aktif anti malaria artemisinin hasil fermentasi Bacillus polymixa AT12, Jurnal Biosains dan Bioteknologi Indonesia (in press).

Misra LN, Ahmad A, Thakur RS, Jakupovic J. 1993. Bisnor-cadinanes from Artemisia annua and definitive 13C NMR assignments of β-arteether. Phytochem 33(6):1461-1464.

Parwati, T., Bustanussalam, P. Simanjuntak 2003. Studi pendahuluan mikroba endofit penghasil senyawa terpen dari tanaman Artemisia annua, Jurnal Ilmu Keframasian Indonesia vol. 1, no 1, 2003. 27 – 32.

Petrini O, Sieber TN, Toti L, Viret O. 1992. Ecology Metabolite Production, and Utilization in Endophytic Fungi. Wilwy-Liss Press. 1- 10

Ranasinghe, A., Sweatlock J.D., Cooks, R.G., 1993. A rapid screening method for artemisinin ad congeners using MS/MS: search for new analogues in Artemisia annua, J. Nat. Prod. 56 (4); 552 - 563

Zheng, G. Q. 1994. Cytotoxic terpenoids and flavonoids from Artemisia annua. Planta Med. 60:54-57



  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian J Pharm indexed by:

analytics View My Stats