Effect of pentagamavunon-O on rat kidney’s glutathione s-transferase in-vivo with 1-chloro-2, 4-dinitro benzene as a substrate

Abdul Rohman, Sudibyo Martono, Supardjan A. Margono


Glutathione S-transferase (GST) is a detoxifying enzyme which plays an important role in glutathione conjugation reaction with electrophilic xenobiotic on phase two metabolism. In this research, the effect of oral administration of PGV-0 on rat kidney’s GST activity was investigated.
To represent GST activity isolated from rat kidney, 1-chloro-2,4-dinitro benzen (CDNB) was used as a substrate on conjugation with glutathione (GSH) spectrophotometrically. The activity of rat kidney’s GSTs from untreated rat was used as control, Based on data, it can be concluded that PGV-0 showed an inhibitory effect on rat kidney’s GSTs. The optimum effect was obtained at 40 mg/kgBW with % inhibition value of 16,16.
Key words : Pentagamavunon-0, Glutathione S-transferase, 1-chloro-2,4-dinitro benzene

Full Text:



Ana, D., 2001, Pengaruh Pentagamavunon-0 Terhadap Aktivitas Enzim Glutation S-transferase Kelas Phi Ginjal Tikus, Skripsi, Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta.

Boyland, E. and Chasseaud, L.F., 1969, Role of glutathione and glutathione S-transferase in mercapturic acid biosynthesis, Adv. Enzymol., 32: 173-219.

Commandeur, J.M.N., Stijntjes G., and Vermeulen, N.P.E., 1995, Enzymes and transport systems involved in the formation and disposition of glutathione S-conjugates, Pharmacol. Rev., 47:(2), 271-330.

Habig, W.H., Pabst, M.J., and Jakoby, W.B., 1974, Glutathione-S-Transferase The first Enzymatic Step in Mercapturic Acid Formation, J. Biol. Chem. 249: 7130-7139.

Harper, H.A., Rodwell, V.W., and Mayes, P.A., 1977, Biokimia (Review of Physiological Chemistry),diterjemahkan oleh Martin Muliawan, Edisi 16, 52-57, Penerbit Buku Kedokteran E.G.C.,Jakarta.

Lundgren, B., Meijer, J., and De Pierre, J.W., 1987, Characterization of the induction of cytosolic and microsomal epoxide hydrolases by 2-ethylhexanoic acid in mouse liver, Drug Metab.Dispos., 15: 114-121.

Sardjiman, 2000, Synthesis of some new series of curcumin analogues , antioxidative, antiinflammatory,antibacterial activities and qualitative structure-activity relationship, Dissertation, Gadjah Mada University, Yogyakarta, Indonesia.

Sudibyo, M.,1999, Uji Penghambatan Aktivitas Glutation S-transferase Liver Tikus oleh 2,5-bis-(4-hidroksi-3-metoksi benzilidin ) siklopentanon dan Dua Analognya, Lembaga Penelitian, Universitas Gadjah Mada,Yogyakarta.

Sudibyo, M., 2000, Inhibition of Glutathione S-Transferase by Curcumin and its Derivatives, Moleculer Mechanism and Qualitative Structure-Activity Relationship, Dissertation, Gadjah Mada University, Yogyakarta, Indonesia.

Sudibyo, M. dan Supardjan, 2001, Pengaruh Obat-Obat Antiinflamasi Pada Aktivitas Enzim Glutation Stransferase Kelas mu dan pi, Laporan Penelitian Project Grant-QUE Project Batch-I, Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta.

DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp33-36


  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian J Pharm indexed by:

analytics View My Stats