Sub chronic toxicity asssay of aqueous extract of the leaves of Nerium indicum Mill. on male white mice (Ratus norvegicus) strain wistar

Citra Prasetyawati, Imono Argo D, Mae Sri Hartati W, Subagus Wahyuono


Aqueous extract of the leaves of Nerium indicum Mill. is traditionally used to treat cancer. The extract has been consumed for relatively a long time, consequently major organs are often intoxicated. Therefore, this study was aimed to determine sub chronic toxicity of the aqueous extract on male mice. Toxic symptoms, major organs intoxicated and haematological changes due to treatment of aqueous extract of the leaves of N. indicum are determined.
Twenty male mice (185-250 g, strain wistar) are divided into 4 groups [I, treated with aquadest (3 ml/200 g BW); II, treated with water extract (5%, 3ml/200 g BB); III, treated with water extract (10%, 3 ml/200 g BB); and IV, treated with water extract (20%, 3 ml/200 g BB)]. Toxic symptoms are observed after 3 hours of treatment; body weight, haematological measurement, food intake, urine chemistry, SGPT, ureum, and histopathological pictures are also observed.
The results of the study do not show any toxic symptoms, the body weight and food intake of the mice increase (I-IV). There are no major organs intoxicated; however a slight increase of leukocyte (WBC, white blood cell) count is clearly showed by group IV.
It is concluded that consuming aqueous extract (20%, the highest concentration) of N. indicum for 30 days does not elicit toxic effect in mice. Any changes occur are still at the normal range.
Keywords: Nerium indicum Mill., water extract, toxicity, sub chronic

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Anonim, 2001, Cancer Rehabilitation Organization,,Oktober 2001

Galey, E. G., 1996, Diagnosis of oleander poisoning in live stock, J. Vet. Diagn. Invest. July; 8(3); 358-64

Nafrialdi dan Gan, S., 1995, Antikanker dalam Farmakologi dan Terapi, edisi ke IV, 686-701, diterjemahkan oleh Widianto, M. B., ITB, Bandung.

King, R.J.B., 2000, Cancer Biology, Second Edition, Pearson Education Limited, London

Moecherdiantiningsih dan Kodim, N., 2000, Kanker Serviks Uteri dan metode deteksi dini dan efektif, Medika, (4), tahun XXVI, 254-6

Reynolds JEF (ed), 1989, Martindale: The extra pharmacopoeia, 29th ed., the Pharmaceutical Press, London

Shaw, D., and Pearn, J., 1979, Oleander poisoning, Med. J.Aust, 2 : 267-9

Wahyuningsih, M. S. H, Wahyuono, S., 1999, Brine Shrimp Lethality test (BST) dari bahan tanaman yang dilaporkan dapat digunakan sebagai antikanker, Majalah Obat Tradisional, 3(6), 142-51

Wahyuningsih, M. S. H., Wahyuono, S., Artama, W. T., 2000, Isolasi dan identifikasi senyawa bioaktif dari daun Nerium indicum Mill., Majalah Farmasi Indonesia, 11(2), 86-95

Wahyuningsih, M. S. H., Wahyuono, S., Artama, W. T., 2001, Sitotoksisitas oleandrin, senyawa bioaktif hasil isolasi dari daun Nerium indicum Mill. terhadap sel mieloma, Berkala Ilmu Kedokteran, Desember, 32(4), 235-241

Wahyuningsih, M. S. H., Bolhuis, R. L. H., Oostrum, R. G., Boersma, A. W. M., Ganjar, I. G., Mubarika, S., Hamann, M. T., Wahyuono, S. (2002). In vitro cytotoxicity of oleandrin isolated from the Nerium indicum Mill. against human cancer cell lines, Laporan Penelitian



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