CAPABILITY OF WATER AND ETHYL ACETATE FRACTIONS OF BENALU (Dendrophthoe L.f Ettingsh) LEAVES TO DISSOLVE CALCIUM KIDNEY STONE IN VITRO, DETERMINED USING FAST NEUTRON ACTIVATION METHOD

Sasmito ., Darsono ., Zainul Kamal, Joko Kristanto

Abstract


Kidney stone disease is the second major causal leading to kidney failure. Treatment to the disease could be by surgery, ultravibration and either traditional or modern medecines. Dendrophthoe falcata (L.f) Ettingsh grow, as a parasite, on the mindi (Melia azeradach), a plant has yet been used to cure kidney disorder caused by calcium kidney stone. Dendrophthoe falcata (L.f) Ettingsh leaves powder was extracted by ethanol (70%) and re-extracted by ethyl acetate. The compound content in water and ethyl acetate fractions were analyzed by thin layer chromatography (TLC) using cellulose as the stationary phase and a mixture of butanol: acetic acid: water (4:1:5) as mobile phase. The TLC data showed that water and ethyl acetate fractions contained flavonoid compounds. Kidney stone was analyzed using Infra Red Spectrophotometer to identify its chemical contents. The result showed that the kidney stone consisted of calcium and magnesium carbonate/phosphate. The Fast Neutron Activation analysis was utilized to determine the solubility of kidney stone. The result exhibited that the maximum solubility of kidney stone was achieved in water fraction with the concentration of 20 % or in ethyl acetate fraction with the concentration of 40 %. The solubility of kidney stone in water fraction was higher than that in ethyl acetate fraction.

Key word : Kidney stone , Dendrophthoe falcata , Water fraction, Ethyl acetat fraction.


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References


Brown, C.B., 1989, Manual Penyakit Ginjal, diterjemahkan oleh Moch. Sadikin dan Winarsi Rudiharso, 204-211, Binarupa Aksara, Jakarta.

Chozin. A., Wahjoedi. B., Pudjiastuti, 1992, Informasi Penelitian Batani dan Fitokimia Tanaman Benalu, Warta Tumbuhan Obat Indonesia, I-2, Vol. 4 no. 4 Jakarta.

Doudon, M., Prostat, M.F., and Reveiland, R. J., 1978, Analyse des Calcus par Spectrophotometrie Infrarouge Advantage et Limites dela Methode, 475-489, Vol 36. Ann Biol, Clim.

Manitto, P., 1992, Biosintesis Produk Alami, Diterjemahkan oleh Koensoemardiyah, 434-435,449, IKIP Semarang Press, Semarang.

Markham, K.R., 1988, Cara Identifikasi Flavonoid, diterjemahkan oleh Kosasih Padmawinata, 1-54, Penerbit ITB, Bandung.

Pramono, S. 1988, Buku Temu Risalah Temu Ilmiah 1987 Fakultas Farmasi UGM, 341-343, Fakultas Farmasi UGM Yogyakarta.

Pramono S., Sumarmo, Wahyono, S., 1993, Flavonoid Daun Sonchus arvensis Senyawa aktif Pembentuk Komplek dengan Batu Ginjal Berkalsium, Warta Tumbuhan Obat Indonesia, 5-7, Vol.2, No.3, Jakarta.

Pudjorahardjo, S.D., 1994, Petunjuk Aktivasi Neutron Cepat, Bidang Fisika Nuklir dan Atom, PPNY Batan, Yogyakarta.

Tresnawati, 1999, Daya Melarutkan Fraksi Etil Asetat dan Fraksi Air Rimpang Temulawak (Curcuma zanthorrhiza Roxb) Terhadap Batu Ginjal Kalsium In Vitro, Skripsi, Fakultas Farmasi Universitas Gadjah Mada, Yogyakarta.

Wijayakusuma, H., 1992, Tanaman Berkhasiat Obat di Indonesia, Jilid II, 94-95, Pusataka Kartini, Jakarta.

Yusutardi, 1997, Berbagai Upaya Mencegah Batu Ginjal, Sidowayah, 29-31, No.23, Tahun VI.




DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp120-127

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