The effect of mengkudu fruit methanolic extract and methanolic residual fraction on GLUT-4 protein elevation

Aguslina Kirtishanti, Ryanto Budiono

Abstract


Investigating in the Diabetes Mellitus drug which one having good activity and tolerance to patients are still in researchers’ concern. One of the main topics now days is the use of mengkudu plants as a traditional medicine of Diabetes Mellitus. This research was aimed to determine the increase of GLUT-4 protein in type 2 Diabetes Mellitus rats after given methanolic extract and methanolic residual of mengkudu fruit. The male rats were diabetic induced with exogenous i.p. insulin for 10 days. After showing hyperglycemic effect, the rats were given orally methanolic extract and methanolic residual fraction of mengkudu fruit for 4 days. On the fifth day, fasting blood glucose was measured and the rats were sacrificed to take the thigh muscle tissue for immunohistochemical calculation. The result showed that methanolic extract and methanolic residual of mengkudu fruit increase the amount of GLUT-4 protein, but can not reduce fasting blood glucose levels of male white rats.

Key words : mengkudu fruit (Morinda citrifolia L.), methanolic extract and methanolic residual, type 2 Diabetes Mellitus, GLUT-4 protein


References


Cheng, J. T., Liu, I. M., Chi, T. C., Su, H. C., and Chang, C. G., 2001, Stimulation of Insulin Release in Rats by Die-Huang-Wan, A Herbal Mixture Used in Chinese Traditional Medicine, J. of Pharmacy and Pharmacology 53 : 273 - 276

Ducluzeau, P. H., Perretti N., Laville, M., Andreelli, F., Vega, N., Riou, J. P., and Vidal, H., 2001, Regulation by Insulin of Gene Expression in Human Skeletal Muscle and Adipose Tissue, Diabetes 50 : 1134 – 1142

Goldfine, A. B., 2001, Hospital Practice : Type 2 Diabetes : New Drugs, New Perspectives,The McGraw-Hill Companies, Boston, 1 – 6

Guyton, A. C., and Hall, J. E., 2000, Textbook of Medical Physiology, 10th edition, W.B. Saunders Company, Philadelphia, p. 884 – 886

Hendromartono, 2002, The Role of PPAR Activator on Insulin Resistance, Surabaya Diabetes Update-XI, Surabaya, 1 – 4

Kirtishanti A., 2004, Efek Jus Buah Mengkudu (Morinda citrifolia L.) Terhadap Peningkatan Jumlah Protein GLUT-4 Pada Tikus Putih Jantan Hiperglikemik (Analog Diabetes Mellitus Tipe 2), Tesis, Universitas Airlangga, Surabaya

Lay, S. L., Lefrere I., Trautwein, C., Dugail, I., and Krief, S., 2002, Insulin and Sterolregulatory Element-binding Protein-1c (SREBP-1c) Regulation of Gene Expression in 3T3-L1 Adipocytes, J. Biology Chemistry 277 (38) : 35625 – 35634

Martoprawiro, S. S, and Sandhika Willy, 2002, Prinsip-prinsip Imunomikroskopis, Universitas Airlangga, Surabaya.

Mendez, J. D., and Ramos, H. G., 1994, Animal Models in Diabetes Research, Archieves of Medical Research, 367 – 375

Wu Yang Chang, Hsu, J. H., Liu, I. M., Liou, S. S., Su, H. C., and Cheng, J. T., 2002, Increase of Insulin Sensitivity in Diabetic Rats Received Die-Huang-Wan, A Herbal Mixture Used in Chinese Traditional Medicine, Acta Pharmacologica Sinica 23(12) : 1181 – 1187

Zainuddin M, 2000, Metodologi Penelitian, Universitas Airlangga, Surabaya




DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp170-177

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian J Pharm indexed by:

web
analytics View My Stats