STANDARDIZATION OF CELERY LEAVES PREPARATION (Apium graveolens L.) BY THIN LAYER CHROMATOGRAPHIC – DENSITOMETRY USING APIGENIN AS PARAMETER

Djatmiko M., Pramono S.

Abstract


In order to apply a standardization method on herbal medicines or pharmaceuticals, a thin layer chromatographic-densitometry was used to determine apigenin as the active constituent of celery extract formulated in a capsule dosage form. Silica gel GF 254 and chloroform – methanol – water (70 – 30 – 6,5 v/v) were used as stationary and mobile phases, respectively. The areas of apigenin were evaluated at 340 mm using a TLC-Scanner.

The limit of detetion was 3 g and the accuracy was 89,6% after first extraction prosedure applied on celery capsules. The apigenin content was 0,017 %.

Key Words : Apium graveolens L., apigenin, TLC – densitometry.


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DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp59-64

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