Isolation and identification of eurycomanone from akar pasak bumi (Eurycoma longifolia, Jack) and its antiangiogenic activity

Sugiyanto ., Nina Salamah, Mae Sri Hartati, Farida Hayati, Pinus Jumariyatno


Pasak bumi is one of the Indonesian original plants having anti cancer activity. One compound of akar pasak bumi, i.e eurycomanone was proven to have cytotoxic effect on cancer cell culture by apoptosis induction on HeLa cells. Therefore, this study was aimed to isolate and identify euycomanone from akar pasak bumi and to look at the anti angiogenic activity of the compounds on corio alantois membrane (CAM) of chicken embryo induced by bFGF.

The akar pasak bumi was ground and was macerated with methanol. The macerate was fractioned by mean of VLC. Six fractions was obtained. The second fraction exhibited two spots on a TLC system, one spot was cochromatographed with eurycomanone as the reference standart and another spot exhibited blue fluorecence at 254 nm. This second fraction, then, was chromatographed on a preparative scale TLC to separate isolate A. Anti angiogenic test was performed for Isolate A at concentration series of 5, 10, 15, 20, 40 μg/mL respectively. Egg embrio (8-9 days) were divided into 8 groups. The first group was implanted paper disc only, the second group were given bFGF on Tris HCl buffer and the third group were given bFGF (on Tris HCl buffer) + 0,8 % DMSO. The fourth to eighth group were induced by 10 ng bFGF and the test preparation at the respective concentration. After incubation at 39 °C for 3 days, each egg was opened, the content of each was aspired and discarded, the CAM sticked to its shell was examined macroscopically and microscopically for the formation of new blood vessels.

Based on spectral data of UV, IR, 1H NMR, 13C NMR and MS of isolate A and comparison with the spectral data of the standart, the isolate A was eurycomanone. Eurycomanone inhibited angiogenesis at concentration of 10 μg/mL.

Key words: antiangiogenic, angiogenesis, E.longifolia, eurycomanone,CAM.


Chan K.L. , Chee-Yan Choo, Noor Rain Abdullah, and Zakiah Ismail, 2004, Antiplasmodial studies of Eurycoma longifolia Jack using the lactate dehydrogenase assay of Plasmodium falciparum, Journal of Ethnopharmacology 92, 223-227

Hanahan, D and Weinberg, R.A., 2000, The Hallmark of Cancer, Cell.,100:57-68.

Jenie R.I., Meiyanto, dan E., Murwanti, R., 2006, Efek antiangiogenik ekstrak etanolik daun sambung nyawa (Gynura procumbens (Lour.) Merr pada membran korio alantois (CAM) embrio ayam, Majalah Farmasi Indonesia, 17(1):50-55

Jing, R.W., Mao-de L., and Jian-guang Z., 2006, Anticancer effects of Chinese herbal medicine, science or myth?, J.Zhejiang Univ SCIENCE B. 7(12): 1006-1014

Kardono, L.B.S., Angerhofer, C.K., Tsauri, S., Padmawinata, K., Pezzuto, and J.M., Kinghorn, D., 1991., Cytotoxic and antimalarial constituents of the roots of Eurycoma longifolia, Journal of Natural Products 54, 1360-1367

Knighton, D., D. Ausprunk, D.Tapper, and J. Folkman, 1977, Avascular and Vascular Phases of Tumor Grrowth in The Chick Embrio. Br.J. Cancer 35 no. 347-356.

Mustofa dan Qomariah N., 2004, Aktivitas Antiplasmodial in vitro dan Sitotoksik Akar Pasak Bumi terhadap malaria di Kalimantan Selatan, Medika, 3, 147-152

Nurkhasanah and Pihie A.H.L., 2006, Apoptotic Cell Death Induced by Eurycomanone (Eurycoma longifolia, Jack) in Human Cervical Carcinoma Cells, Proceeding of International Conference on Mathematics and Natural Sciences, Kuala Lumpur

O’Reilly, M.S., Holmgren, L., Shing, Y., Chen, C., Rosenthal, R.A., Moses, M., Lane, W.S., Cao, Y., Sage, E.H., and Folkman, J., 1994, Angiostatin: A novel angiogenesis inhibitor that mediates the suppression of metastases by a Lewis lung carcinoma, Cell., 79: 315–328.

Ribatti, D., Gulandaris, A.,Bastaki, M., Vacca,A., Lurlalo, M., Roncali, L., and Presta, M.,1997, New Model for Study of angiogenesis and Antiangiogenesis in The Chick Embryo Chorioallantoic Membrane: The Gelatin Sponge/ Chorioallantoic Membrane Assay, J Vasc Res, 34: 455-463.

Sengupta, S., Toh S.A., Sellers L.A., Skepper, J.N., Koolwijk, P., Leung, H.W., Yeung H.W., Wong, R.N.S., Sasisekharan R., and Fan, T.P.D., 2004, Modulating Angiogenesis : The Yin and the Yang in Ginseng, Circulation. 110:1219-1225.

Sun, X., Ding, Y., Yan, X., Wu, L., Li, Q., Ceng, N., Qiu, Y., and Zhang, M., 2004, Angiogenic synergistic effect of basic fibroblast growth factor and vascular endothelial growth factor in an in vitro quantitative microcarrier-based three-dimensional fibrin angiogenesis system, World J Gastroenterol 2004; 10(17): 2524-2528



  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian J Pharm indexed by:

analytics View My Stats