Isolation and identification of kaempferol from jangkang (Homalocladium platycladum (F. Muell) Bailey) leaves and its antibacterial activity

Maulita Cut Nuria, Wahyono ., Ratna Asmah Susidarti


Isolation and  identification  of  kaempferol  from  jangkang  (Homalocladium platycladum (F.  Muell)  Bailey)  leaves  and  its  activity  against  B.  subtilis ATCC 9466 dan S. typhi ATCC 1408 has been done. The dried ground leaves (300.04 gram)  were  macerated  with  petroleum  ether,  chloroform  and  methanol respectively.  The  methanol  extract  was  the  most  active  extract  against  both bacteria.  Further  separations  of  this  extract  using preparative  thin  layer chromatography  yielded  flavonoid  glycoside.  Its  aglicon  was  identified  as kaempferol  based  on  its  spectroscopic  data  (MS,  IR, and  UV-Vis)  and  UV spectrum  (densitometry).  This  compound  shows  iradical  zone  of  6.62  mm (Bacillus  subtilis)  and  6.27  mm  (Salmonella  typhi)  at  concentration  of  200 µg/disk.

Key words : kaempferol, jangkang, glycoside, aglicon

Full Text:

PDF 1-8


Anonim, 2008, Indonesia Country Profile 2007, hal. 17, Depatemen Kesehatan, Jakarta.

Djumidi, 1997, Inventaris Tanaman Obat Indonesia IV, hal. 101-102, Departemen Kesehatan Badan Penelitian dan Pengembangan Kesehatan, Jakarta.

Erazo, S., Munoz, O., Garcia, R., Lemus, I., Backhouse, N., Negrete, R., Feliciano, A.S., and Delporte, C., 2002, Constituents and Biological Activities from Muehlenbeckia hastulata, Z. Naturforsch, 57c, 801-804.

Lim, Y.H., Kim, I.H., and Seo, J.J., 2007, In vitroActivity of Kaempferol Isolated from Impatiens balsamina alone and in Combination with Erythromycin or Clindamycin against Propionibacterium acnes, The Journal of Microbiology, Vol. 45, No. 5, 473-477.

Markham, K.R., 1988, Cara Mengidentifikasi Flavonoid, diterjemahkan oleh Kosasih Padmawinata, hal. 15, 38-50, Penerbit ITB, Bandung.

Ozcelik, B., Orhan, D.D., Ozgen, S., and Ergun, F., 2008, Antimicrobial Activity of Flavonoids against Extended-Spectrum β-Lactamase (ESβL)-Producing Klebsiella pneumoniae, Tropical Journal of Pharmaceutical Research, 7 (4), 1151-1157.

Yamin, 2010, Isolasi dan Identifikasi Senyawa Antibakteri dari Daun Jakang (Muehlenbeckia platycada Meissn), Tesis, Fakultas Farmasi Universitas Gadjah Mada, Yogyakarta.

Yen, C.T., Hsieh, P.W., Hwang, T.L., Lan, Y.H., Chang, F.R., and Wu, Y.C., 2009, Flavonol Glycosides from Muehlenbeckia platycada and Their Anti-inflammatory Activity, Chem. Pharm. Bull, 57(3), 280-282.



  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian J Pharm indexed by:

analytics View My Stats