Scientific Aspects of Water Extract Katola (Arcangelisia flava L. Merr) as Antidiarrhea Agent in Mina South Sulawesi
Water extract of katola (Arcangelisia flava L. Merr) stem is traditionally used in communities Muna Distric for the treatment of many diseases, including anti diarrhea. According to the information of Muna Distric Health Office, diarrhea is one of the 10 groups diseases in this district. The objective of this study was conducted to determine the potential antimicrobial, acute toxicity and identified of the water extract of bioactive compounds and alkaloid relative concentration content. Infection of microbial was induced by oral administration of Shigella flexneri ATCC 12022 1 x 108 CFU/mL. Boiling water of katola dose 12 mg/kg, 24 mg/kg and 48 mg/kg body weight and the antibiotic ampicillin 24 mg/kg administered 2 times daily for 5 days. The number of S. flexneri in feces were determined every day. The toxicity of water extract of katola stem is determined based on the value of LD50 acute oral toxicity testing followed by guidelines OECD 423. The bioactive compounds was determined by bioautografi method and the concentration alkaloid relative content was calculated as berberine hydrochloride. The results showed that boling water extract of katola stem did not cause toxic symtoms, with LD50 > 31.5 g/kg body weight (unclassified) or 104 times the dose therapy of human. In vitro, the Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC)value of the boiling water were 1.2 and 2.4%, respectively. In vivo, the boiling water dose 48 mg/kg body weight given twice a day have an effect on eradication the S. flexneri 100% by day 5. Bioactive compounds of antimicrobial in the water extract is alkaloid berberine HCl. A cocentration of berberine hydrochloride in water exract stem and leaves dosage use of the public were 16.7% and 0.75% respectively.
Key words: Katola, toxicity, anti diarrhea, antimicroba, Arcangelisia flava L. Merr
Full Text:PDF 283-289
Anonim, 2009, Data 10 besar penyakit di masyarakat, Dinas Kesehatan Kabupaten Muna.
Anonim, 2010, OEDC Guideline for Testing of Chemicals, Acute Oral Toxicity–Acute Toxic Class Method, Cernakova, M., and Kostalova D., 2002, Antimicrobial activity of berberine a constituent of Mahonia aquifolium, Folia Microbiol (Praha)., 47: 375-378
Hegnauer, R., 1969, Chemotaxonomie der Planzen, Edisi V, 73-86, Birkhäuser Verlag, Basel und Stuttgart.
Hwang, B.Y., Roberts, S.K., Chadwick, L.R., Wu C.D., and Kinghorn, A.D., 2003, Antimicrobial Constituents from Goldenseal (the Rhizomes of Hydrastis Canadensis) Against Selected Oral Pathogens. Planta. Med. J. ;69 (7): 23-627.
Keawpradub, N., Dejadisai, S., and Yuenyongsawa S., 2005, Antioxidant and Cytotoxic Activities of Thai Medicinal Plants Named Khaminkhruea: Arcangelisia flava, Coscinium blumeanum and Fibraurea tinctoria, Songklanakarin J. Sci. Tech., 27 (Suppl. 2) : 455-467.
Lu, F.C., 1991, Toksikologi Dasar : Asas, Organ, Sasaran, dan Penilaian Resiko, diterjemahkan oleh Edi Nugroho,1995, Edisi II, UI-Press, Jakarta.
Scazzocchio, F., Cometa, M.F., Tomassini, L., and Palmery, M., 2001, Antibacterial Activity of Hydrastis Canadensis, Extract and its Major Isolated Alkaloids, Planta. Med.,67 (6): 561-564.
Subekti., 2001, Shigella sp. Suveillsnce in Indonesia:Emergence or Reemergence of S.dysentriae. Bagian Mikrobiologi Program Mikrobiologi Section of the Enteric Diseases Program at U.S.Naval Medical Research Unit No.2, Jakarta,Indonesia.
Tanaka, T., Kaneda, Y., and Torii, M., 1991, In vitro effects of berberine sulphate on the growth and structure of Entamoeba histolytica, Giardia lamblia and Trichomonas vaginalis. Ann. Trop. Med. Parasitol.,85: 417-425.
Tjaniadi, P., Lesmana, M., Subekti, D., Machpud, N., Komalarini, S., and Santoso, W., 2003, Antimicrobial Resistance of Bacterial Pathogens Associated With Diarrheal Patients in Indonesia. Am. J. Trop. Med. Hyg., 68: 666-670.
Ukumu, L., Samiu, W., and Kobe, W., 2009, Pembuat Ramuan Obat dari batang tumbuhan katola, Komunikasi lisan.
Weber, H. A., and Joseph, M., 2002., Extraction and HPLC Analysis of Alkaloids in Goldenseal Application, Consumer Products and Drug Manufacturing/QA/QC, Agilent Technologies, Inc.Wilmington USA.
- There are currently no refbacks.
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Indonesian J Pharm indexed by:View My Stats