Component  and  antibacterial  activity  of  crystal  fraction  from Zingiber zerumbetessential oil

Sri Mulyani

doi:10.14499/indonesianjpharm0iss0pp178-184

Component and antibacterial activity of crystal fraction from Zingiber zerumbetessential oil

Sri Mulyani

Abstract

The aims of the study was to determine of the crystal fraction from Zingiber zerumbet essential oils. The components examination was performed by GC-MS method using the solvent n-hexane and anti bacterial activity was tested against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 35218 using agar diffusion method with coconut oil solvent. The components that can be identified are α-humulene, caryophyllene oxide, β-selinene, and zerumbone. Crystal fraction solution able to inhibit of S. aureusATCC 25923 at the concentration 25 % and up to 50 % has not been able to inhibit E. coli ATCC 35218.

Key words : Zingiber zerumbet, Lempuyang gajah, Crystal fraction, anti-bacterial

Full Text:

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References

Agusta A, 2000. Minyak Atsiri Tumbuhan Tropik Indonesia, 89., ITB, Bandung

Ahmad, B. A., Siddig, I. A., Adel, SAZ., Manal, M. E., and Syam M. M., 2008, Anticancer and Antimicrobial Activites of Zerumbone from the Rhizomes of Zingiber zerumbet.. International Journal of Pharmacology4 (4), 301-304

Anonim, 1986, Medicinal Herbs Index in Indonesia., PT Eisei Indonesia ., 348-349

Bustanul, A., 2007, Uji Daya Antibakteri Minyak Atsiri Lempuyang Gajah (Zingiber zerumbet) terhadap Staphylococcus aureus ATCC 25923 dan Escherichia coli ATCC 35218, Skripsi, Fakultas Kedokteran UGM.

Carson, C. F, Mee, B. J., and Riley, T. V, 2002, Mechanism of Action of Melaleuca alternifolia (Tea Tree) Oil on Staphylococcus aureus Determined by Time Kill, Lysis, Leakage, and salt tolerance assays and electron microscopy. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=127210

Jang, D. S., Min H. Y., Kim M. S., Han, A. R., Wudono, T., Jeohu G. H., Kong, S. S., Lee, S. K., and Seo, E. K, 2005, Humulens Derivative from Zingiber zerumbetwith the Inhibitory Effect on Lipopolysacharide-induced Nitric-oxide Production., Chem Pharm Bull (Tokyo) 53 (7), 829-831. http://agris.fao.org/agris-search/search/display.do?f= 1995/ US/US95207.xml;US9443476 Jawets, E, Melnick, J. L, and Adelberg, E. A, 1996,Mikrobiologi Kedokteran., Edisi XX., 234-240, Diterjemahkan oleh Nugroho Edi dan Maulany., EGC. Jakarta.

Knobloch, K, Weis, N., and Weigand, H., 1986, Mechanism of Antimicrobial Activity of Essential Oil, Planta Medica6, 556.

Lechat-Vahirua, I., Francois, P., Menut, C., Lamaty, G., and Bessiere, G. M., 1993, Essential oils from three Polynesian Zingiberaceae, J Riv. Ital.EPPOS, V 4, N spec. Num., p. 740-2.

http://www.google.co.id/#hl=id&&sa=X&ei=L591TOX6M4KivQP5lpC2Bg&ved=0CBEQBSgA&q=Le+Chat-essential+oils+from+three+polynesian+ zingiberaceae & spell=1&fp=dc4b9ba5e001c2f8

Syahrizal, M., 2006, Kajian Kimia Zerumbon daripada Zingiber zerumbetSmith, Thesis Fakulti Sains Universiti Teknologi Malaysia. http://eprints.utm.my/5325/ Sawangjaroen, N., Phongpaichit, S., Subhadhirasakul, S., Vissuthi, M., Srisuwan, N., and Thammapalerd, N., 2006, The Anti-amoebic Activity of Some Medicinal Plants Used by AIDS Patients in Southern Thailand, Parasitol Res. 98 (6) : 588-92. Epub 2006 Jan 31. http://www.ncbi.nlm.nih.gov/pubmed/16447069

Sakinah, S., Tri Handayani., and Hawariah, A., 2007, Zerumbone Induced apoptosis in liver cancer cells via modulation of Bax/Bcl-2 ratio, Cancer Cells International, 7:4, doi: 10. 1186/1475-2867.7-4. Pub. 3 April 2007. http://cancerci.com/ content/7 /1/4/abstract

DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp178-184