PROTOPLAST SUSPENSION CULTURE OF LEAVES MESOPHYLOF Centella asiatica (L.) Urban AND QUALITATIVE ANALYSIS ASIATICOSIDE
Centella asiatica leaves has been used as diuretic, antihypertensi, anti leprae, skin infection, burning skin and celloid. Cells suspenson culture of mesophyll of Centella asiatica leaves were carried out in three steps: isolation, purification, and cell culture. The aim of this research is to investigate the influences of leaves position (age of leaves) and concentration of macerozyme R-10 for cells number and viability, and the influence of sucrose concentration for cells growth and biosynthesis of asiaticoside. Isolation and purification of mesophyll cell has been performed using factorial completely randomized design. The first factor was leaf position (age of leaves), and the second factor was concentration of macerozyme. Collected data were analysed using Anova and Duncan’s test at 0.0 – 2.5 – 5.0 and 7.5 per cent respectively. Asiaticoside produced by cell biomass were analyzed qualitatively using thin layer chro- matography. The result revealed that mesophyll cells could be used as explant for suspension culture. The highest biomass produced with highest viability were found in cells isolated from second leaves treated with 0.1% macerozyme (1.32 . 107 cells/ml). Addition of sucrose to suspension culture medium produced higher packed cells volume (pcv) percentage than no sucrose was added, (0% sucrose gave pcv 8.5%, 2.5% sucrose pcv 22%, sucrose 5% pcv 21.5%, and 7.5% sucrose pcv 15.75%). The asiaticoside production not only depended on sucrose, because without any sucrose was added, the asiaticoside was also available..
Key Words: Cell suspension culture, mesophyll, Centella asiatica (L.) Urban, asiaticoside
Bhojwani,S.S., and Razdan,M.K., 1983, Plant Tissue Culture: Theory and Practice, Elsevier Publishing Company,Inc., New York.
Dixon,R.a., 1987, Plant Cell Cultures-A Practical Approach, Dept. of Biochemistry, Royal Holloway College.
George,E.F. and Sherrington,F.D., 1984, Plant Propagation by Tissue Culture, Handbook and Directory of Commercial Laboratories, Exergetics Ltd., England.
Gomez,K.A. and Gomez,A.A., 1995, Prosedur Statistik untuk Penelitian Pertanian , Diterjemahkan oleh Susilo H., UI Pres, Jakarta.
Hadini,H., 1997, Hibridisasi somatik pisang ambon dan kavendis secara in vitro, Tesis, Program Pasca Sarjana UGM, Yogyakarta.
Hefnawi,H.E., 1962A, Treatment Kheloid with Asiaticoside, Dermatologica, 124: 387-392, Dermatology Department, Cairo University, Cairo.
Hefnawi,H.E., 1962B, Madecassol in the Treatment of Chronic Ulcers, Egyptian Medical Association, 45:7-8.
Huseman,W., 1984, Photoautotrophic Cell Cultures, in: Cell Culture and Somatic Cell Genetic of Plants (Vasil,I.K., ed.), Academic Press, London.
Kao,K.N., Constabel,C., Michayluk,M.R., Gamborg,O.L., 1974, Plant Protoplast Fusion and Growth of Intergeneric Hybrid Cells, Planta, 120:215-227.
Komatsu,S., Taizo, Keisuke, 1996, Phosphorylation of a Protein is Related to the Regeneration of Rice Cultured Suspension Cells, Plant Cell Physiol.,37b:748-753.
Morris,P., Sragg,A.H., Smart,N.J., Stafford,A., 1987, Secondary Product Formation by Cell Suspension Culture, in: Plant Cell Culture-A practical approach, Dept. of Biochemistry, Royal Holloway College.
Pramono,S., 1992, Profil Kromatogram Ekstrak Herba Pegagan yang Berefek Antihiperttensi, Warta Tumbuhan Obat Indonesia, Vol. I, 2:37-39.
Salisbury,F.B. and Ross,C.W., 1995, Fisiologi Tumbuhan , Jilid I, Diterjemahkan oleh Diah,R.L. dan Sumaryono, Penerbit ITB, Bandung.
Soegigardjo,C.J., 1993, Bioteknologi Kultur Jaringan Tanaman, Jilid III, Program Studi Ilmu Farmasi, Program Pasca Sarjana UGM, Yogyakarta.
Staba,E.J., 1980, Plant Tissue Culture A Source of Biochemicals, CRC Press, Boca Raton, Florida.
Suryowinoto,M., 1996, Pemuliaan Tanaman Secara In Vitro, PAU Bioteknologi UGM, Yogyakarta.
Tyler,V.E., Brady,L.R., Roberr,J.E., 1976, Pharmacognosy, Seventh Ed., Lea&Febiger, Philadephia.
Vasil,I.K., 1984, Cell Culture and Somatic Cell Genetic of Plants , Vol.I, Academic Press Inc., New York.
Widowati, Pudjiastuti,L., Dea,I., Dian,S., 1992, Beberapa Informasi Khasiat, Keamanan, dan Fitokimia Tanaman Pegagan (Centella asiatica (L.)Urban. ), Warta Tumbuhan Obat Indonesia, Vol.I, 2: 39-42.
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