Phalerin, a new benzophenoic glucoside isolated from the methanolic extract of Mahkota Dewa [Phaleria macrocarpa (scheff). Boerl.] leaves

Mae Sri Hartati W; Sofia Mubarika; Ibnu G Gandjar; Mark T Hamann; KV Rao; Subagus Wahyuono

doi:10.14499/indonesianjpharm0iss0pp51-57

Phalerin, a new benzophenoic glucoside isolated from the methanolic extract of Mahkota Dewa [Phaleria macrocarpa (scheff). Boerl.] leaves

Mae Sri Hartati W, Sofia Mubarika, Ibnu G Gandjar, Mark T Hamann, KV Rao, Subagus Wahyuono

Abstract

Mahkota Dewa [Phaleria macrocarpa (Scheff). Boerl.] is used traditionally to treat cancers in Indonesia. Extract methanol of the leaves displayed a small LC50 value (63.16 μg/ml) on BST (Brine Shrimp Lethality test) assay; therefore a phytochemical study of this extract was undertaken. A new benzophenoic glucoside was isolated from the methanol extract and identified as 4,5-dihydroxy,4’-methoxybenzophenone-3-0-β-D-glucoside (Phalerin) based on its spectroscopic data. Phalerin was cytotoxic to myeloma cell line (NS-1) (in vitro) having IC50 of 83 μg/ml or 1.9 x 10-1 mM.
Key words: Phaleria macrocarpa, Phalerin, myeloma

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References

Chulia A.J. and Kaouadji M., 1985. Depressoside, nouvel iridoide isole de Gentiana depressa. J. Nat. Prod., 48(1)Jan-Feb: 54-8

Bloor S.J. and Qi L., Cytotoxic saponins from New Zealand Mysrsine species. J. Nat. Prod., 57(10): 1354-60

Bruneton J., 1999, Pharmacognosy, Phytochemistry Medicinal Plants. Intercept Ltd., 3-35

Harmanto N., 1998, Makutodewa Obat Pusaka Para Dewa. Agro Media Pustaka, Jakarta. pp. 7-13

Mae S.H.W., Subagus W, Gemini A, Ika R.A, and Veronika K.,2002, Toxic effect evaluation of fractions obtained from the leaves of Mahkotadewa [Phaleria macrocarpa (Scheff.)

Boerl.] methanol extract by brine shrimp lethality test. J. Trad. Med., 7: 29-33

Matsumoto K., Akao Y., Kobayashi E., Ito T., Ohguchi K., Iinuma M., and Nozawa Y. 2003, Cytotoxic benzophenone derivatives from Garcinia sp. display a strong apoptosisinducing effect against human leukemia cell line. Biol. Pharm.Bull. April;26(4): 569-71

McLaughlin J.L, and Rogers L.L., 1998, The use of biological assays to evaluate botanicals. Drug Information Journal, 32: 513-24

Mosmann, T., 1983, Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods, 65: 55-63

Silverstein R.M., Bassler G.C., and Morrill T.C., 1981, Spectrometric Identification of Organic Compounds, John Wiley & Sons, New York

Silverstein R. M. and Webster F.X., 1998, Spectrometric Identification of Organic Compounds, 6th edition, John Wiley & Sons, New York

Wang K.J., Zhang Y.J., and Yang, C.R., 2003. New Compounds from Fagopyrum dibotrys. Asian Symposium on Medicinal Plants, Spices and Other Natural Products XI, Kunming, China, October 26-30, 2003

William R.B, Hoch J, Glass T.E, Evans R, Miller J.S, Wisse J.H, and Kingston D.G., 2003, A novel cytotoxic guttiferone analogue from Garcinia macrophylla from the Suriname rain forest. Planta Medica, Sept.;69(9): 864-6

DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp51-57