Antiangiogenic effect of sambung nyawa leaves (Gynura procumbens (Lour.) Merr.) etanolic extract on chick embryo chorioallantoic membrane (CAM)

Riris Istighfari Jenie, Edy Meiyanto, Retno Murwanti


Antiangiogenesis (inhibition of new blood vessels formation) has become a strategy to inhibit cancer development lack of nutrition and oxygen supply. The aim of the present research is to investigate antiangiogenesis effect of ethanolic extract of Gynura procumbens (Lour.) Merr. Leaves in situ using chick embryo chorioallantoic membrane (CAM). Eight to 9 days old fertilized chicken eggs were treated with b-FGF (angiogenesis inductor) and extracts. Eggs were then incubated for 3 days in order to observe its angiogenesis response (new blood vessels converged toward the implant).

The results showed that the ethanolic extract of G.procumbens could inhibit angiogenesis in a dose-dependent manner. Doses 10, 20, 40, 80 ug gave angiogenesis response of (in percent) 82.32 ± 6.33; 68.38 ± 6.24; 56.48 ± 11.61; 41.43 ± 7.46 (p<0.05), respectively. These results indicate a potential antiangiogenic effect of the extract.

Key words: antiangiogenic, CAM, G.procumbens.

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Igura, K., Ohta T., Kuroda Y., and Kaji K., 2001, Resveratrol and quercetin inhibit angiogenesis in vitro, Cancer Lett, 171 (1): 11-6.

Kerbel, R., and Folkman, J., 2002, Clinical Translation of Angiogenesis Inhibitors, Nat. Rev., 2: 727-739.

Keshet, E., and Ben-Sasson, S. A., 1999, Anticancer drug targets:approaching angiogenesis, J. Clin. Invest., Vol.104, No.11, 1497-1501.

King, R.J.B., 2000, Cancer Biology, Ed. 2, Pearson Education, England.

Masferrer, J.L, and Leahy K.M, et al, 2000, Antiangiogenic and Antitumor Activities of Cyclooxygenase-2 Inhibitors, Cancer Res, 60:1306-1311.

Matter, A., 2001, Tumor angiogenesis as a therapeutic target, Drug Discovery Today, Vol 6, No. 19, hal 1005-1020.

Ribatti, D., Gulandris, A., Bastaki, M., Vacca, A., Iurlaro, M., Roncali, L., and Presta, M., 1997, New Model for the Study of Angiogenesis and Antiangiogenesis in the Chick Embryo Chorioallantoic Membrane: The Gelatin Sponge/Chorioallantoic Membrane Assay, Journal of Vascular Research, 34:455-463.

Ribatti, D., Leali, D., Vacca, A., Giuliani, R., Gualandris, A., Roncali, L., Nolli, M.L., and Presta, M., 1999, In Vivo Angiogenic Activity of Urokinase; Role of Endogenous Fibroblast Growth Factor-2, J. Cell. Sci., 112, 4213-4221.

Schneider, K.A., 1997, Cancer Genetics, Encyclopedia of Human Biology, Vol. 2, Hal 311-320.

Singh, R.P., Dhanalakshmi, S., Tyagi, A.K., Chan, D.C.F., Agarwal, C.,and Agarwal, R., 2002, Dietary Feeding of Silibinin Inhibits Advance Human Prostate Carcinoma Growth in Athymic Nude Mice and Increases Plasma Insulin-like Growth Factorbinding Protein-3 Levels1, CANCER RESEARCH, 62, 3063–3069.

Sugiyanto, Sudarto, B., Meiyanto, E., Nugroho, A.E., and Jenie, U.A., 2003, Aktivitas antikarsinogenik senyawa yang berasal dari tumbuhan, Majalah Farmasi Indonesia, 14(4):216-225.

Tosetti, F., Ferrari, N., de Flora S., and Albini A., 2002, ‘Angioprevention’: angiogenesis is a common and key target for cancer chemopreventive agents, FASEB J., 16:2-14.



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