Peroxide value and DPPH (diphenyl picril hydrazil hydrate) free radical scavenger activity of Knema laurina methanol extract

Praptiwi ., Puspa Dewi, Mindarti Harapini


The aim of the study were to determine the peroxide value and anti DPPH free radical scavenger activity of Knema laurina (Myristicaceae) methanol extract as indicator og antioxidant properties. Peroxide value wasdetermined by iodometri-titration method while anti DPPH free radical was determined by spectrophotometry. Based on phytochemical screening, the chemical compounds of K. laurina were essential oil, sterol, triterpen, tannin, peroxide sugar, alkaloid and saponin. Peroxide value (POV) of K. laurina methanol extract was 158.07 peroxide/1kg sample, while POV of α- tocopherol was 363.96 peroxide/1kg sample. It mean that the inhibition of oxidation process of K. laurina methanol extract was better that that of α-tocopherol so the peroxide formed was lower. The IC50 of K. laurina methanol extract was 39.72 ppm, while IC50 of vitamin C was 12.20 ppm. The result showed that methanol extract of K. laurina at the concentration of 39.72 ppm inhibit 50% of free radical DPPH activity. Based on the peroxide value and IC50 of K. laurina methanol extract, it can be concluded that K. laurina methanol extract had potential activity as anti-DPPH free radical and could be acted as reductor in oxidation process.

Key words : Knema laurina, peroxide value (POV), free radical DPPH

Full Text:



Boer E, Hidebrand JW, Alonzo DS, and Fundrer JM. 1995. Koordersiodendron Engl. In : Timber Trees: Minor Commercial Timbers. (RHMJ Lemmens, Soerianegara and WC Wong, Ed). Backhuys Publishers. Leiden.

Burkill IH. 1935. A Dictionary of the Economic Products of the Malay Peninsula. The Crown Agents for the Colonies. London.

Cuiley J. 1984. Methodology for Analysis of Vegetables and Drugs. Fac. Of Pharmacy. Bucharest.Rumania.

Dalimartha S and Soedibyo M. 1998. Awet Muda. Dengan Tumbuhan Obat dan Diet Suplemen. Trubus Agriwidya. Jakarta

Dyatmiko W, MH Santosa, and AF Hafid. 2000. Aktifitas Penangkapan Radikal Bebas Dalam Sistem Molekuler dan Seluler Sari Air Rimpang Tanaman Obat Zingiberaceae. Lembaga Penelitian Universitas Airlangga. Pusat Penelitian Obat Tradisional Univ. Airlangga. Surabaya.

Heyne K. 1987. Tumbuhan Berguna Indonesia. Badan penelitian dan Pengembangan Kehutanan. Departemen Kehutanan. Departemen Kehutanan, Indonesia.

Hudson, BJF. 1990. Food Antioxidants. Elsevier Applied Science. New York.

Robinson T. 1991. Kandungan Organik Tumbuhan Tinggi. Penerbit ITB. Bandung.

Williams, S. (Ed.). 1984. Official Methods of Analysis of the Assoc. O fAnalytical Chemists. Assoc. of Analytical Chemists, Inc. Arlington, USA.

Yen, Gow-Chin and Hii-Yin Chen. 1995. Antioxidant ity of various tea extracts in relation to their antimutagenicity. J. Agric. Food. Chem 43: 27-32.



  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian J Pharm indexed by:

analytics View My Stats