Ethanolic extract of Areca catechu seeds inhibit proliferation and induce apoptosis on MCF-7 cells

Edy Meiyanto, Ratna Asmah Susidarti, Sri Handayani, Fitria Rahmi


Areca catechu seed contains antioxydant substances, supposed to have anticancer property. This research therefore addressed to examine the inhibitory effect of Areca catechu seed ethanolic extract (EP) on proliferating breast cancer cells, MCF-7. Areca catechu seed ethanolic extract (EP) standardization was done according to the standard of BPOM. Areca catechu seed powder extraction was done using ethanol 96%. Cytotoxic assay – to get the value of IC50 and to prevent the cell proliferation (using doubling time assay) – was carried out by using MTT assay. Apoptosis observation was done by acrydine orange- etidium bromide staining method (double staining). The result showed that treatment with Areca catechu seed ethanolic extract (25-100 µg/m) for 48 h caused 13-84% growth inhibition (IC 5077 µg/mL) of the cells, while arecoline (ARE) treatment (10-500 µg/mL) showed 8-73% inhibition (IC50 180 µg/mL). The extract also inhibited cell proliferation and induced apoptosis. These results conclude that Areca catechu seed ethanolic extract (EP) possesses antiproliferative effect through growth inhibition and apoptosis induction.
Key words:MCF-7, Areca catechu, antiproliferative


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BPOM, 2000, Parameter Standar Umum Ekstrak Tumbuhan Obat, Depkes RI.

Chakraborty, M., Das, K., Dey, G., and Mitra, A., Unusually high quantity of 4-hydroxybenzoic acid accumulation in cell wall of palm mesocarps, Biochemical Systematics and Ecology, 34, 509-513.

Conze, D., Weiss, L., Regen, P.S., Bhushan, A., Weaver, D., Johnson, P., and Rincon, M., 2001, Autocrine Production Of Interleukin 6 Causes Multi Drug Resistance In Breast Cancer Cells, Cancer Research,61, 8851–8858.

Crawford, K.W. and Bowen, W.D., 2002, Sigma-2 Receptor Agonists Activate a Novel Apoptotic Pathway and Potentiate Antineoplastic Drugs in Breast Tumor Cell Lines, Cancer Res., 62, 313–322.

Fisher, D.E., 1994, Apoptosis in Cancer Therapy: Crossing The Threshold, Cell, 78, 539-542.

Hakem, R., and Harrington, L., 2005, The Basic Science of Oncology, Cell Death, McGrow-Hill Medical Companies, 4th Edition, New York, 194-204.

Hanahan,D.,and Weinberg, R.A.,2000, The Hallmarks of Cancer,Cell, 100, 57-70.

Kampa, M., Alexaki, V.I., Notas, G., Nifli, A.P., Nistikaki, A., Hatzoglou, A., Bakogeorgou, E., Kouimtzoglou, E., Boskou, D., Gravanis, A., and Castanas, E., 2003, Antiproliferative and apoptotic effects of selective phenolic acids on T47D human breast cancer cells: potential mechanisms of action, Breast Cancer Res,6, R63-R74.

Kitagawa, S., 2006, Inhibitory Effect of Polyphenols on P-Glycoprotein-Mediated Transport, Biol. Pharm. Bull.,29, 1-6.

Lee, K.K., and Choi, J.D., 1999, The Effects of Areca Catechu L Extract on Anti-Inflammation and Anti-Melanogenesis, International Journal of Cosmetic Science21, 275–284.

Lu, H., and Waxman, D.J., 2005, Antitumor Activity of Methoxymorpholinyl Doxorubicin: Potentiation by Cytochrome P450 3A Metabolism, Mol Pharmacol, 67, 212–219.

Menchetner, E., Kyshtoobayeva, A., Zonis, S., Kim, H., Stroup, R., Garcia, R., Parker, R.J., and Fruehauf, J.P., 1998, Levels of Multidrug Resistance (MDR1) P-Glycoprotein Expression by Human Breast Cancer Correlate with in Vitro Resistance to Taxol and Doxorubicin, Clinical Cancer Research, 4, 389-398.

Mortensen, K., Skouv, J., Hougaard, D.M., and Larsson, L.I., 1999, Endogenous Endothelial Cell Nitric-oxide Synthase Modulates Apoptosis in Cultured Breast Cancer Cells and Is Transcriptionally Regulated by p53, J Biol Chem, 274, 37679-37684.

Mosmann, T., 1983, Rapid Colorimetric Assay for Cellular Growth and Survival: Aplication to Proliferation and Citotoxicity Assays,J. Immunol Methods, 65, 55-63.

Onuki, R., Kawasaki, H., Baba, T., and Taira, K., 2003, Analysis of a Mitochondrial Apoptotic Pathway Using Bid-Targeted Ribozymes in Human MCF7 Cells in the Absence of a Caspase-3-Dependent Pathway, Antisense and Nucleic Acid Drug Development, 13, :75-82.

Prunet, C., Lemaire-Ewing, S., Ménétrier, F., Néel,D., and Lizard, G., 2005, Activation of caspase-3-dependent and -independent pathways during 7-ketocholesterol- and 7β-hydroxycholesterol- induced cell death: A morphological and biochemical study, Journal of Biochemical and Molecular Toxicology, 19, 311-326.

Shapiro, G.I., and Harper, J.W., 1999, Anticancer Drug Targets: Cell Cycle and Checkpoint Control, J. Clin. Invest., 104, 1645-1653.

Wang, C.K., and Lee, W.H., 1996, Separation, Characteristics, and Biological Activities of Phenolics in Areca Fruit, J. Agric. Food Chem., 44, 2014 -2019.

Wickenden, J.A., Clarke, M.C.H., Rossi, A.G., Rahman, I., Faux,, S.P., Donaldson, K, and MacNee, W., 2003, Cigarette Smoke Prevents Apoptosis through Inhibition of Caspase Activation and Induces Necrosis, Am. J. Respir. Cell Mol. Biol., 29, 562–570.

Yi, W., Fischer, J., Krewer, G., and Akoh, C.C., 2005, Phenolic Compounds from Blueberries Can Inhibit Colon Cancer Cell Proliferation and Induce Apoptosis, J. Agric. Food Chem.,53, 7320 -7329.

Zhu, J., Nozell, S., Wang, J., Jiang, J., Zhou, W.,and Cheng, X., 2001, p73 cooperates with DNA damage agents to induce apoptosis in MCF7 cells in a p53-dependent manner, Oncogene, 20, 4050-4057.



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