Ethanolic extract of Areca catechu seeds inhibit proliferation and induce apoptosis on MCF-7 cells

Edy Meiyanto; Ratna Asmah Susidarti; Sri Handayani; Fitria Rahmi

doi:10.14499/indonesianjpharm0iss0pp12-19

Ethanolic extract of Areca catechu seeds inhibit proliferation and induce apoptosis on MCF-7 cells

Edy Meiyanto, Ratna Asmah Susidarti, Sri Handayani, Fitria Rahmi

Abstract

Areca catechu seed contains antioxydant substances, supposed to have anticancer property. This research therefore addressed to examine the inhibitory effect of Areca catechu seed ethanolic extract (EP) on proliferating breast cancer cells, MCF-7. Areca catechu seed ethanolic extract (EP) standardization was done according to the standard of BPOM. Areca catechu seed powder extraction was done using ethanol 96%. Cytotoxic assay – to get the value of IC50 and to prevent the cell proliferation (using doubling time assay) – was carried out by using MTT assay. Apoptosis observation was done by acrydine orange- etidium bromide staining method (double staining). The result showed that treatment with Areca catechu seed ethanolic extract (25-100 µg/m) for 48 h caused 13-84% growth inhibition (IC 5077 µg/mL) of the cells, while arecoline (ARE) treatment (10-500 µg/mL) showed 8-73% inhibition (IC50 180 µg/mL). The extract also inhibited cell proliferation and induced apoptosis. These results conclude that Areca catechu seed ethanolic extract (EP) possesses antiproliferative effect through growth inhibition and apoptosis induction.
Key words:MCF-7, Areca catechu, antiproliferative

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DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp12-19