Isolation and structure identification of new alkaloids from the sponge Rhabdastrella rowi

Triana Hertiani, RuAngelie Edrada, Rob W.M. Van Soest, Sudarsono ., Peter Proksch


Chemical investigation on marine sponge Rhabdastrella rowi collected from Bali, Indonesia has been performed. This study was aimed to isolate and to identify structures of the sponge secondary metabolites as well as to test their cytotoxicity activity on mouse lymphoma cell line L5178Y.

Isolation procedure was performed by using different chromatography techniques. NMR spectroscopy and mass spectrometry methods were used to identify the compounds chemical structures. Cytotoxicity of the isolates was tested on mouse lymphoma cell line L5178Y by using the microculture tetrazolium (MTT) assay.

This study yielded two new alkaloids, quinolin-4-ol (1) and quninazolin-4-amine (2) which were found as minor constituents of Rhabdastrella rowi. Both compounds were observed as inactive against mouse lymphoma cell line L5178Y.

Key words: Rhabdastrella rowi, sponge, alkaloids


Bourguet-Kondracki, M.L., Longeon, A., Debitus, C., and Guyot, M., 2000, New cytotoxic isomalabaricane-type sesterterpenes from the New Caledonian marine sponge Rhabdastrella globostellata, Tetrahedron Lett., 41, 3087-3090

Carmichael, J., DeGraff, W.G., Gazdar, A.F., Minna, J.D., and Mitchell, J.B., 1987, Cancer Res., 47, 943-946

Debitus, C., Guella, G., Mancini, I., Walkedre, J., Guemas, J.G., Nicolas, J.L., and Pietra, F., 1998, Quinolones from a bacterium and tyrosine metabolites from its host sponge, Suberea creba from the Coral Sea, J. Mar. Biotechnol., 6, 136-141

Fouad, M., Edrada, R.A., Ebel, R., Wray, V., Müller, W.E.G., Lin, W.H., and Proksch, P., 2006, Cytotoxic Isomalabaricane Triterpenes from the Marine Sponge Rhabdastella globostellata, J. Nat. Prod., 69, 211-218

Li, L.-Q., Qu, Z.X., Wang, Z.M., Zeng, Y.L., Ding, G.S., Hu, G.J., and Yang, X.Y., 1979, Studies on a new antiarrhythmic drug changrolin-4-(3',5'-bis [(N-pyrrolidinyl) methyl]-4'-hydroxyanilino)-quinazoline, Sci. Sin. (Engl. Ed.), 22 (10), 1220-1228

Lu, F., Deng, Z., Li, J., Fu, H., Van Soest, R.W.M., Proksch, P., and Lin, W., 2004, Isomalabaricane-type compounds from the marine sponge Rhabdastrella aff. distincta, J. Nat. Prod., 67(12), 2033 -2036

Proksch, P., Edrada, R., and Ebel, R., 2002, Drug from the seas – current status and microbiological implications, App. Microbiol. Biotechnol, 59, 125-134

Rao, Z., Deng, S., Wu, H., and Jiang, S., 1997, Rhabdastrellic acid-A, a novel triterpenoid from the marine sponge Rhabdastrella globostellata, J. Nat. Prod., 60, 1163-1164

Tasdemir, D., Mangalindan, G.C., Concepción, G.P., Verbitski, S.M., Rabindran, M.M., Greenstein, M., Hooper, J.N.A., Harper, M.K., and Ireland, C.M., 2002, Bioactive isomalabaricane triterpenes from the marine sponge Rhabdastrella globostellata, J. Nat. Prod., 65, 210-214

Uriz, M.J. In: Hooper, J.N.A., and Van Soest, R.W.M. (Eds.), 2002, Systema porifera: A guide to the classification of sponges, Kluwer Academic/ Plenum Publisher, New York, 108-126

Van Soest, R.W.M., 1989, The Indonesian sponge fauna: a status report, Netherlands Journal of Sea Research, 23(2), 223 – 230.



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