Structure identification of potential compound as selective renal anticancer isolated from Nerium indicum Mill. Leaves

Mae S.H. Wahyuningsih, Sofia Mubarika, Mark T. Hamann, Ibnu G. Gandjar, Subagus Wahyuono

Abstract


Oleandrin is one of cardenolida compound isolated from an active fraction of Nerium indicum Mill leaves. (fam. Apocynaceae), which have cytotoxic effect on several human cancer cells, and also to normal cells in vitro. Another compound which was potential as renal anticancer has also been isolated from N. indicum. However, its chemical structure has not been discovered. The aim of this study was to identify the potential compound as selective to renal cancer present in the leaves of N. indicum.
The potential compound was isolated from the active fraction using Preparative TLC and structure elucidation was done by using spectroscopic methods (UV, IR, MS and NMR).
Base on this spectra and by comparison with oleandrin data, it was indicated that the potential compound as Renal anticancer was as 16,17-dehydrodeacetyl-5a-oleandrin.
Key word: N.indicum, oleandrin, cytotoxic, renal anticancer, spectroscopic

References


Begum, S., Sultana, R., and Siddiqui, B. S., 1997, Triterpenoids from the leaves of Nerium oleander, Phytochem., 44 (2) 329-332

Begum, S., Siddiqui, B. S., Sultana, R., Zia, A., and Suria, A., 1999, Bio-active cardenolides from the leaves Nerium oleander, Phytochemistry, 50, 435-438

Boisio, M. L., Esposito, M., and Metlo, F.,1993, Separation and identifying features of the cardiac aglycones and glycosides of Nerium oleander L. flowers by thin-layer chromatography,Minerva Med., 84(11),627-632

Friebolin, H., 2005, Basic One-and Two-Dimensional NMR Spectroscopy, Fourth, Completely Revised and Updated Edition, Wiley-VCH Verlag GmbH & Co. KgaA, Weinheim, Germany.

Hartwel, J. L., 1982, Plant Used Against Cancer, Quarterman Publications, Inc., Lawrence,Massachusetts, p.407

Hembing, W. H. M., 1993, Tanaman Berkhasiat Obat Di Indonesia, Jilid ke-2, 74-75, Pustaka Kartini

Mc. Conkey, D. J., Lin, Y., Nutt, L. K., Ozel, H. Z., and Newman, R. A., 2000, Cardiac glycosides stimulate Ca2 + increases and apoptosis in androgen-independent metastatic human prostate adenocarcinoma cell, Cancer Res, 60(14), 3807-3812.

Nafrialdi dan Gan. S., 1995, Antikanker dalam Farmakologi dan Terapi, Edisi ke IV, p.686-701, Bagian Farmakologi Fakultas Kedokteran UI, Jakarta.

Pathak, S., Multani, A. S., Narayan, S., Kumar, V., and Newman, R. A., 2000, Anvirzel, an extract of Nerium oleander, induced cell death in human but not murine cancer cells, Anticancer Drugs, 11(6), 455-463

Prasetyawati, C., Donatus, I. A., Wahyuningsih, M. S. H., and Wahyuono, S., 2004, Uji toksisitas sub kronis infusa daun Nerium indicum Mill. pada tikus putih jantan (Rattus norvegicus)galur Wistar, M. F. I., 15 (1), 13-19

Siddiqui, S., Begum, S., Siddiqui, B. S., and Hafeez, Z., 1989, Kanerin and 12-13-dihydroursolic acid, two new pentacyclic teriterpenes from the leaves of Nerium oleander, J. Nat. Prod.,52 (1), 57-62

Siddiqui, B. S., Sultana, R., Begum, B. S., Zia, A., and Suria, A., 1997, Cardenolides from the methanolic extract of Nerium oleander leaves possessing central nervous system depressant activity in mice, J.Nat. Prod., 60, 540-544

Silverstein, R. M., and Webster, F. X., 1998, Spectrometric Identification of Organic Compounds, 6th edition, John Wiley & Sons, Inc., New York. Silverstein, R. M., Bassler, G. C., and Morrill, T. C., 1991, Spectrometric Identification Of Organic Compounds, fifth ed., John Wiley & Sons, Inc., New York.

Wahyuningsih, M. S. H., Wahyuono, S., and Artama, W. T., 2000a, Isolasi dan Identifikasi Senyawa Bioaktif dari daun Nerium indicum Mill. Majalah Farmasi Indonesia, 11(2), 86-95

Wahyuningsih, M. S. H., Wahyuono, S., and Artama, W. T., 2000b, Efek Sitotoksik Oleandrin,Senyawa Bioaktif Hasil Isolasi dari daun Nerium indicum Mill. Terhadap Sel Mieloma.B.I.K., 32(4), 235-241

Wahyuningsih, M. S. H., 2006, Selektivitas dan Mekanisme Antikanker 5a-oleandrin dan dehidrodeasetil-5a-oleandrin Hasil Isolasi daun jure (Nerium indicum Mill.), Disertasi S3,Sekolah Pascasarjana, Universitas Gadjah Mada, Yogyakarta. 16,17-

Zia, A., Siddiqui, B. S ., Begum, S., Siddiqui, S., and Suria, A., 1995, Studies on the constituents of the leaves of Nerium oleander on behavior pattern in mice, J. Ethnopharmacol., 49 (1),33-39.




DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp57-64

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