ANTI-STRESS ACTIVITY OF SOME PLANTS EXTRACTS OF THE NORTH CAUCASUS FLORA

Dmitry Igorevich Pozdnyakov; Similla Adzhiahmetova; Nadezhda Chervonnaya; Andrey Mamleev; Eduard Oganesyan

doi:10.14499/indonesianjpharm31iss2pp131

ANTI-STRESS ACTIVITY OF SOME PLANTS EXTRACTS OF THE NORTH CAUCASUS FLORA

Dmitry Igorevich Pozdnyakov, Similla Adzhiahmetova, Nadezhda Chervonnaya, Andrey Mamleev, Eduard Oganesyan

Abstract

To date, stress is a common medical and socially significant disease that requires rational pharmacotherapeutic correction.

The anti-stress properties of ethnolic and aqueous extracts obtained from leaves of Ribes nigrum L., inflorescences of Gaillardia pulchella Foug., stems of Lysimachia punctata L were studied in this work.

Acute stress was modeled by immobilization of rats for 2 hours. The test-extracts were administered per os prophylactically in a dose of 1/20 of LD₅₀ (2000 mg/kg). The following parameters were evaluated: organs mass coefficient (adrenal glands, thymus, spleen), the number of stomach erosion; the biochemical changes in the blood serum (adrenaline, cortisol, total protein and glucose concentration); the mitochondrial function parameters in brain and myocardium (evaluation of mitochondrial pore transitional permeability opening and mitochondrial membrane potential).

70% ethanol extract from Gaillardia pulchella Foug. inflorescences has the highest anti-stress activity, the course application of which contributed to the normalization of the weight index of organs, a decrease in glucose concentration by 64.5% (p <0.05), and cortisol by 73.7% (p <0.05) and adrenaline - 78.9% (p <0.05) in the blood, an increase in total protein level by 62.5% (p <0.05) and stabilization of mitochondrial function.

The study showed a high anti-stress activity of 70 % ethanol extract from Gaillardia pulchella Foug. inflorescences.

Keywords

stress; plant extracts; mitochondria

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DOI: http://dx.doi.org/10.14499/indonesianjpharm31iss2pp131