ANTI-STRESS ACTIVITY OF SOME PLANTS EXTRACTS OF THE NORTH CAUCASUS FLORA
Abstract
To date, stress is a common medical and socially significant disease that requires rational pharmacotherapeutic correction.
The anti-stress properties of ethnolic and aqueous extracts obtained from leaves of Ribes nigrum L., inflorescences of Gaillardia pulchella Foug., stems of Lysimachia punctata L were studied in this work.
Acute stress was modeled by immobilization of rats for 2 hours. The test-extracts were administered per os prophylactically in a dose of 1/20 of LD50 (2000 mg/kg). The following parameters were evaluated: organs mass coefficient (adrenal glands, thymus, spleen), the number of stomach erosion; the biochemical changes in the blood serum (adrenaline, cortisol, total protein and glucose concentration); the mitochondrial function parameters in brain and myocardium (evaluation of mitochondrial pore transitional permeability opening and mitochondrial membrane potential).
70% ethanol extract from Gaillardia pulchella Foug. inflorescences has the highest anti-stress activity, the course application of which contributed to the normalization of the weight index of organs, a decrease in glucose concentration by 64.5% (p <0.05), and cortisol by 73.7% (p <0.05) and adrenaline - 78.9% (p <0.05) in the blood, an increase in total protein level by 62.5% (p <0.05) and stabilization of mitochondrial function.
The study showed a high anti-stress activity of 70 % ethanol extract from Gaillardia pulchella Foug. inflorescences.
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Yeap SK, Beh BK, Ali NM, 2015. Antistress and antioxidant effects of virgin coconut oil in vivo. Exp Ther Med 9(1):39–42. doi:10.3892/etm.2014.2045
Doreddula SK, Bonam SR, Gaddam DP, Desu BS, 2014. Phytochemical analysis, antioxidant, antistress, and nootropic activities of aqueous and methanolic seed extracts of ladies finger (Abelmoschus esculentus L.) in mice. ScientificWorldJournal 2014:519848. doi:10.1155/2014/519848
Joshi T, Sah SP, Singh A. 2012, Antistress activity of ethanolic extract of Asparagus racemosus willd roots in mice. Indian Journal of Experimental Biology. 50(6):419–424.
Gold SM, 2005.The role of stress-response systems for the pathogenesis and progression of MS. Trends Immunol 26(12):644–652.
Tan SY, Yip A, 2018. Hans Selye (1907-1982): Founder of the stress theory. Singapore Med J 59(4):170–171. doi:10.11622/smedj.2018043
McEwen BS, 2006. Protective and damaging effects of stress mediators: central role of the brain. Dial. in Clin. Neurosci.Stress. 8:367–381.
McEwen BS, Chattarji S, 2007. Handbook of Neurochemistry and Molecular Neurobiology. Springer-Verlag; 2007. pp. 572–593.
Bergh C, Udumyan R, Fall K, Almroth H, Montgomery S, 2015. Stress resilience and physical fitness in adolescence and risk of coronary heart disease in middle age. Heart. 101(8):623–629. doi:10.1136/heartjnl-2014-306703
Montgomery SM, Berney LR, Blane D, 2000. Prepubertal stature and blood pressure in early old age. Arch Dis Child. 82(5):358–363. doi:10.1136/adc.82.5.358
Upadhyay G, Khoshla S, Kosuru R, Singh S, 2016. Anxiolytic, antidepressant, and antistress activities of the aqueous extract of Cinnamomum tamala Nees and Eberm in rats. Indian J Pharmacol. 48(5):555–561. doi:10.4103/0253-7613.190752
Ben Othman M, Han J, El Omri A, Ksouri R, Neffati M, Isoda H, 2013. Antistress Effects of the Ethanolic Extract from Cymbopogon schoenanthus Wild in Tun Growing isia. Evid Based Complement Alternat Med. 2013:737401. doi:10.1155/2013/737401
Pozdnyakov DI., Pozdnyakova AE, Adzhiahmetova SL, Chervonnaya NM, Zolotych DS, Lyakhova NS, Miroshnichenko KA, 2019. Antihypoxic and anti-ischemic properties of the North Caucasus flora plant extracts. Bol. latinoam. Caribe plantas med. Aromát. 18:504-517
Hsu F, Spannl S, Ferguson C, Hyman AA, 2018. Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria. Elife 7:e32282. doi:10.7554/eLife.32282
Dementieva TM, Frolova OO, Evseeva OS, Selivanova S L, 2014 Research of antioxidant activity of decoctions of bark and shoots of Babylonian willow and its hybrid with white willow. Healthcare Of The Far East 1:83-87.
Dorovskikh VA, Simonova NV, Tonkonogova MS, 2015. Comparative evaluation of phytoadaptogens under oxidative stress .Bull. physical and Res. Pat. 55:95-100.
Sullivan PG, Krishnamurthy S, Patel SP, Pandya JD, 2007. Temporal characterization of mitochondrial bioenergetics after spinal cord injury. Journal of neurotrauma. 24:991–999
Boyer PD. 1975.The Enzymes. Academic Press ⅩⅡB: 421
Parvin R, Pande SV, Venkitasubramanian TA, 1965. On the colorimetric biuret method of protein determination. Analytical Biochemistry. 12 (2):219-229
Zhyliuk VI, Mamchur VV, Pavlov S. 2015, Role of functional state of neuronal mitochondria of cerebral cortex in mechanisms of nootropic activity of neuroprotectors in rats with alloxan hyperglycemia. Eksp. i klin. farm. 78: 10-4. (In Russ.)
Patel NB, Galani VJ, Patel BG, 2011. Antistress activity of Argyreia speciosa roots in experimental animals. J Ayurveda Integr Med. 2(3):129–136. doi:10.4103/0975-9476.85551
Kulkarni MP, Juvekar AR, 2008. Attenuation of Acute and Chronic Restraint Stress-induced Perturbations in Experimental Animals by Nelumbo nucifera Gaertn. Indian J Pharm Sci. 70(3):327–332. doi:10.4103/0250-474X.42982
Oladipo GO, Ibukun EO. 2016, BioActivities of Coturnix japonica (quail) egg yolk and albumen against physiological stress. Food Sci Nutr. 5(2):334–343. doi:10.1002/fsn3.397
Bighelli I, Trespidi C, Castellazzi M, 2016. Antidepressants and benzodiazepines for panic disorder in adults. Cochrane Database Syst Rev. 9(9): doi:10.1002/14651858.CD011567.pub2
Kumar S, Madaan R, Sharma A, 2008. Pharmacological evaluation of Bioactive Principle of Turnera aphrodisiaca. Indian J Pharm Sci. 70(6):740–744. doi:10.4103/0250-474X.49095
Moharram FA, El Dib RAEM, Marzouk MS, El-Shenawy SM, 2017. New Apigenin Glycoside, Polyphenolic Constituents, Anti-inflammatory and Hepatoprotective Activities of Gaillardia grandiflora and Gaillardia pulchella Aerial Parts. Pharmacogn Mag. 13(Suppl 2):S244–S249. doi:10.4103/pm.pm_344_16
Abramova ER., Tekeyeva DI., Adzhiakhmetova SL, 2018. The study of polysaccharides of some representatives of the families Primulaceae and Asteraceae. Belikov meetings:104–107. (In Russ.)
Kyosev PA, 2002. A complete reference of medicinal plants. EKSMO – Press :992.
Kim EH, Kim IH, Ha JA, Choi KT, Pyo S, Rhee DK, 2013. Antistress effect of red ginseng in brain cells is mediated by TACE repression via PADI4. J Ginseng Res. 37(3):315–323. doi:10.5142/jgr.2013.37.315
Martínez-Reyes I, Diebold LP, Kong H, 2016. TCA Cycle and Mitochondrial Membrane Potential Are Necessary for Diverse Biological Functions. Mol Cell. 61(2):199–209. doi:10.1016/j.molcel.2015.12.002
Ranchordas MK, Rogerson D, Soltani H, Costello JT, 2017. Antioxidants for preventing and reducing muscle soreness after exercise. Cochrane Database Syst Rev. 12(12) doi:10.1002/14651858.CD009789.pub2
Peng W, Du H, Liu G, 2019. Antistress Effects of San-Huang-Xie-Xin Decoction on Restraint-Stressed Mice Revealed by 1H NMR-Based Metabolomics and Biochemistry Analysis. Oxid Med Cell Longev. 2019:5897675. doi:10.1155/2019/5897675
Apostolova N, Victor VM, 2015. Molecular strategies for targeting antioxidants to mitochondria: therapeutic implications. Antioxid Redox Signal. 22(8):686–729. doi:10.1089/ars.2014.5952.
Kang HW, Lee SG, Otieno D, Ha K, 2018. Flavonoids, Potential Bioactive Compounds, and Non-Shivering Thermogenesis. Nutrients. 10(9):1168. doi:10.3390/nu10091168
DOI: http://dx.doi.org/10.14499/indonesianjpharm31iss2pp131
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