Hepatoprotective effects of Curcumin-Mesoporous Silica Nanoparticles on CCl 4 -induced Hepatotoxicity Wistar rats

Lannie Hadisoewignyo, Ivonne Soeliono, Sandy Budi Hartono, Eka Pramhyrta Hestianah, Sri Rahayu Mahanani

Abstract


It has been reported that curcumin has a hepatoprotective effect, but its low solubility limited its utilization. Recently there was so many emerging research of advanced curcumin formulation, such as nanoparticles curcumin. In our previous study, curcumin has been loaded into mesoporous silica nanoparticles (C-MSN). This study was performed both to evaluate of C-MSN hepatoprotective effect in CCl4-induced rats. Sixteen rats were divided into four groups, namely normal and CCl4 control, curcumin, C-MSN group. Treatment was given according to its group for fourteen days consecutively. At day 14, three hours after the last administration, CCl4 (1,25 ml/kgBB) were administered orally. Twelve hours later the rats were sacrificed, and blood samples were drawn from their hearts. Blood serum examination result revealed that C-MSN caused a significantly lower ALT and AST than CCl4 control group (851±271 U/L vs 1734±275 U/L; 295±155 U/L vs 1348±235 U/L; p<0.05). Its effect on hepatic serum level resembled curcumin group. However, the result was not supported by histology examination which showed a higher number of necrotic hepatic cells in C-MSN group than in the curcumin group (147±9 vs 80±16; p<0.05). From this study, it can be concluded that C-MSN revealed an excellent hepatoprotective property, but it was suspected that MSN itself has the toxic effect on the liver. A further study of MSN toxicity was needed to support its safety use.

 


Keywords


curcumin, curcumin nanoparticles, hepatoprotection, carbon tetrachloride.

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References


Abou Seif, HS., 2016. Physiological changes due to hepatotoxicity and the protective role of some medicinal plants. Beni-Suef Univ. J. Basic Appl. Sci. 5(2): 134–146. doi:10.1016/j.bjbas.2016.03.004

Adaramoye, OA., Odunewu, AO., Farombi, EO., 2010. Hepatoprotective effect of Curcuma longa L. in D-galactosamine induced liver injury in mice: evidence of antioxidant activity. Afr. J. Med. Med. Sci. 39 Suppl 27–34.

Amdalia, PR., Anwar, C., Kurnijasanti, R., 2017. Pengaruh Pemberian Ekstrak Daun Kelor (Moringa oleifera) terhadap Gambaran Histopatologi Sel Hepar Mencit Jantan yang Dipapar Metilmerkuri. J. Basic Med. Vet. 6(1): 1–7.

Černý, D., Lekić, N., Váňová, K., Muchová, L., Hořínek, A., Kmoníčková, E., Zídek, Z., Kameníková, L., Farghali, H., 2011. Hepatoprotective effect of curcumin in lipopolysaccharide/-galactosamine model of liver injury in rats: Relationship to HO-1/CO antioxidant system. Fitoterapia. 82(5): 786–791. doi:10.1016/J.FITOTE.2011.04.003

Farida, Y., Andayani, TM., Ratnasari, N., 2013. Analisis penggunaan obat pada komplikasi sirosis hati. J. Manaj. dan Pelayanan Farm. 4(2): 77–84.

Farzaei, M., Zobeiri, M., Parvizi, F., El-Senduny, F., Marmouzi, I., Coy-Barrera, E., Naseri, R., Nabavi, S., Rahimi, R., Abdollahi, M., 2018. Curcumin in Liver Diseases: A Systematic Review of the Cellular Mechanisms of Oxidative Stress and Clinical Perspective. Nutrients. 10(7): 855. doi:10.3390/nu10070855

Girish, C., Pradhan, SC., 2012. Hepatoprotective activities of picroliv, curcumin, and ellagic acid compared to silymarin on carbon-tetrachloride-induced liver toxicity in mice. J. Pharmacol. Pharmacother. 3(2): 149–55. doi:10.4103/0976-500X.95515

Hartono, SB., Hadisoewignyo, L., Yang, Y., Meka, AK., Antaresti., Yu, C., 2016. Amine functionalized cubic mesoporous silica nanoparticles as an oral delivery system for curcumin bioavailability enhancement. Nanotechnology. 27(50): 505605. doi:10.1088/0957-4484/27/50/505605

Hismiogullari, SE., Hismiogullari, AA., Sunay, FB., Paksoy, S., Can, M., Aksit, H., Karaca, O., 2014. The protective effect of curcumin on carbon tetrachloride induced liver damage. Rev. Med. Vet. (Toulouse). 7–8(165): 194–200.

Kim, Y., You, Y., Yoon, H., Lee, Y-H., Kim, K., Lee, J., Kim, MS., Kim, J., Jun, W., 2014. Hepatoprotective effects of fermented Curcuma longa L. on carbon tetrachloride-induced oxidative stress in rats. Food Chem. 151 148–153. doi:10.1016/j.foodchem.2013.11.058

Kim, YR., Lee, SY., Lee, EJ., Park, SH., Seong, NW., Seo, HS., Shin, SS., Kim, SJ., Meang, EH., Park, MK., Kim, MS., Kim, CS., Kim, SK., Son, SW., Seo, YR., Kang, BH., Han, BS., An, SSA., Lee, BJ., Kim, MK., 2014. Toxicity of colloidal silica nanoparticles administered orally for 90 days in rats. Int. J. Nanomedicine. 9(December): 67–78. doi:10.2147/IJN.S57925

Lee, G-H., Lee, H-Y., Choi, M-K., Chung, H-W., Kim, S-W., Chae, H-J., 2017. Protective effect of Curcuma longa L. extract on CCl4-induced acute hepatic stress. BMC Res. Notes. 10(1): 77. doi:10.1186/s13104-017-2409-z

Li, L., Liu, T., Fu, C., Tan, L., Meng, X., Liu, H., 2015. Biodistribution, excretion, and toxicity of mesoporous silica nanoparticles after oral administration depend on their shape. Nanomedicine Nanotechnology, Biol. Med. 11(8): 1915–1924. doi:10.1016/j.nano.2015.07.004

Manfo, FPT., Nantia, EAN., Kuete, V., 2014. Toxicological Survey of African Medicinal Plants., in: Kuete, V. (Ed.), Toxicological Survey of African Medicinal Plants. Elsevier, London, pp. 323–355. doi:10.1016/C2013-0-15406-2

Marslin, G., Prakash, J., Qi, S., Franklin, G., 2018. Oral delivery of curcumin polymeric nanoparticles ameliorates CCl4-induced subacute hepatotoxicity in wistar rats. Polymers (Basel). 10(5):. doi:10.3390/polym10050541

Nayak, A., Raikar, A., Kotrashetti, V., Nayak, R., Shree, S., Kambali, S., 2016. Histochemical detection and comparison of apoptotic cells in the gingival epithelium using hematoxylin and eosin and methyl green-pyronin: A pilot study. J. Indian Soc. Periodontol. 20(3): 294–8. doi:10.4103/0972-124X.182601

Omosa, LK., Midiwo, JO., Kuete, V., 2017. Curcuma longa., in: Kuete, V. (Ed.), Medicinal Spices and Vegetables From Africa. Elsevier Inc., London, pp. 425–435.

Paolo, P., Kateri, Y., 2017. TESIS Título : “ Efecto hepatoprotector del extracto acuoso liofilizado de Curcuma longa L . en daño hepático agudo inducido por tetracloruro de carbono en ratas albinas ”. TESIS : TÍTULO : “ Efecto hepatoprotector del extracto acuoso liofilizado de Curcu.

Park, E., Jeon, CH., Ko, G., Kim, J., Sohn, DH., Al, EPET., 2000. Protective Effect of Curcumin in Rat Liver Injury Induced by Carbon Tetrachloride. 437–440.

Reyes-Gordillo, K., Shah, R., Lakshman, MR., Flores-Beltrán, RE., Muriel, P., 2012. Hepatoprotective Properties of Curcumin., in: Muriel, P. (Ed.), Liver Pathophysiology: Therapies and Antioxidants. Elsevier Inc., London, pp. 687–704.

Saygili, OK., Paksoy, S., Hismiogullari, SE., Kus, MA., Sunay, FB., Gulcen, B., Ozcan, E., 2016. Prevention of Carbon Tetrachloride-Induced Liver Injury in Rats by Omega-3 Fatty Acids. Acta Medica Mediterr. 33 137–144. doi:10.19193/0393-6384_2017_1_021

Sengupta, M., Sharma, GD., Chakraborty, B., 2011. Hepatoprotective and immunomodulatory properties of aqueous extract of Curcuma longa in carbon tetra chloride intoxicated Swiss albino mice. Asian Pac. J. Trop. Biomed. 1(3): 193–199. doi:10.1016/S2221-1691(11)60026-9

Singh, N., Khullar, N., Kakkar, V., Kaur, IP., 2014. Attenuation of carbon tetrachloride-induced hepatic injury with curcumin-loaded solid lipid nanoparticles. BioDrugs. 28(3): 297–312. doi:10.1007/s40259-014-0086-1

Song, E-K., Cho, H., Kim, J-S., Kim, N-Y., An, N-H., Kim, J-A., Lee, S-H., Kim, Y-C., 2001. Diarylheptanoids with Free Radical Scavenging and Hepatoprotective Activity in vitro from Curcuma longa. Planta Med. 67(9): 876–877. doi:10.1055/s-2001-18860

Tandi, J., 2017. Pola Penggunaan Obat Pada Pasien Penyakit Hati Yang Menjalani Rawat Inap Di Rumah Sakit Umum Daerah Undata Palu. J. Pengemb. Sumber Daya Insa. 02(02): 218–223.

Virgonita, S., Karim, A., 2012. Pola Penggunaan Obat pada Pasien Sirosis Hati di Instalasi Rawat Inap Bangsal Penyakit Dalam Rumah Sakit Dr . Sardjito Yogyakarta CIRRHOSIS IN INTERNAL DISEASE WARD INPATIENT OF. Maj. Farm. 8(3): 218–226.

Wu, S-J., Lin, Y-H., Chu, C-C., Tsai, Y-H., Chao, JC-J., 2008. Curcumin or Saikosaponin a Improves Hepatic Antioxidant Capacity and Protects Against CCl 4 -Induced Liver Injury in Rats. J. Med. Food. 11(2): 224–229. doi:10.1089/jmf.2007.555

Xie, G., Sun, J., ZhonG., G., Shi, L., ZhanG., D., 2010. Biodistribution and toxicity of intravenously administered silica nanoparticles in mice. Arch. Toxicol. 84(3): 183–190. doi:10.1007/s00204-009-0488-x

Zhao, Y., Ma, X., Wang, J., He, X., Hu, Y., Zhang, P., Wang, R., Li, R., Gong, M., Luo, S., Xiao, X., 2014. Curcumin protects against ccl4-induced liver fibrosis in rats by inhibiting HIF-1α through an erk-dependent pathway. Molecules. 19(11): 18767–18780. doi:10.3390/molecules191118767




DOI: http://dx.doi.org/10.14499/indonesianjpharm30iss2pp114-121

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