Metabolite Profiling of Eleutherine palmifolia (L.) Merr. By HPTLC-Densitometry and its Correlation with Anticancer Activities and In Vitro Toxicity

Roihatul Mutiah, Chorida Muhjatul Hadya, Burhan Ma'arif Z.A, Weka Sidha Bhagawan, Rahmi Annisa, Yen Yen Ari Indrawijaya, Fadhila Isma Huwaida, Ria Ramadhani D.A., Retno Susilowati, Imam Taufik

Abstract


Eleutherine palmifolia (L.) Merr. (E. palmifolia) is a plant that used as raw material for herbal medicines. The difference of growing location the medicinal plants affects the difference of metabolite content, It also affects the pharmacological activity and toxicity of the plant. This study aims to determine the profile of metabolites (metabolite fingerprinting), anticancer profiles, toxicity/safety profiles from several different regions in Indonesia for the purpose of authentication, efficacy, safety and quality control of these plants. Samples were sampled from 6 different locations in Indonesia, they were West Java, Central Java, East Java, East Borneo, Central Borneo, and South Borneo. Metabolite fingerprinting was determined by HPTLC-densitometry method, the profile of anticancer activity and toxicity was known by the MTT-ELISA method. The difference between metabolite fingerprinting, anticancer profile, toxicity/safety profile was analyzed by Principal Component Analysis (PCA) and Hierarchical Component Analysis (HCA). Whereas the relation between metabolite fingerprinting, anticancer profile, toxicity/safety profile was analyzed by Partial Least Square (PLS). The results of the metabolite profile analysis with PCA showed that E. palmifolia from 6 different locations gave different metabolite profiles and there were 3 metabolites that had a significant effect on cluster formation. samples are Rf 055, 059, and 044. The results of the HCA analysis showed that the 6 regions are in one cluster the same one. The analysis of PLS-DA showed that the metabolites for anticancer activity are found in Rf 059, 076, 093 and Rf 034. While the metabolites for toxicity are Rf 002, Rf 044 and Rf 059.


Keywords


Eleutherine palmifolia (L.) Merr., Metabolite profile, the profile of anticancer activity, profile toxicity

Full Text:

PDF

References


BPOM RI. 2005. Peraturan Kepala Badan Pengawas Obat dan Makanan Republik Indonesia Nomor: HK.00.05.41.1384 tentang Kriteria dan Tata Laksana Pendaftaran Obat Tradisional, Obat Herbal Terstandar dan Fitofarmaka. Jakarta; Badan Pengawas Obat dan Makanan Republik Indonesia.

Canas RA., Canales J., Muñoz-Hernández C., Granados JM., Ávila C., L, M.,. 2015. Understanding Developmental and Adaptive Cues In Pine Through Metabolite Profiling and Co-Expression Network Analysis. 66:3113-3127.

Chahar MK., Neelu SMP., Dobhal., Yogesh C,. Joshi., 2011. Flavonoids: A versatile source of anticancer drugs. Pharmacogn Rev. 5: 1–12

Dewi NR., Kuncoro H., Rijai L., 2015. Potensi Sitotoksik Ekstrak Air Daun Sirih Hitam (Piper sp.) . Jurnal sains dan Kesehatan. 1 (1)

Dhalwal K., Shinde VM., Namdeo AG., Mahadik KR., 2008. Antioxidant Profile and HPTLC-Densitometric Analysis of Umbelliferone and Psoralen in Aegle Marmelos. Pharmaceutical Biology. 46: 266–72.

Dipiro JT., Talbert RI., Yee GC., Matzke GR., Wells BG., Posey LM., 2005. Pharmacotherapy: A Phatopphysiologic Approach. Sixth Edition. America: The MoGraw-Hill Companies.

Febriyanti AP., Iswarin SJ., Pariwara PW., 2016. Identifikasi dan Eksplorasi Etnomedisina pada Suku Samin di Kabupaten Bojonegoro, Jawa Timur. Journal of Pharmaceutical and Medicinal Sciences. 1: 69-74.

Fiehn O. 2002. Metabolomics-The Link between Genotypes to Phenotypes. Plant Molecular Biology. 48: 155–71.

Fitri Y., Rosidah., Suwarso E., 2014. Effects of Inhibition Cell Cycle and Apoptosis of Sabrang Onion extract (Eleutherine bulbosa (Mill.) Urb.) on Breast Cancer Cells. 6.

Florea A., Busselberg D., 2011. Cisplatin as an Anti-Tumor Drug Cellular Mechanism of Activity Drug Resistence and Induced Side Effect. Cancers. 3: 1351-1330.

Huberger AL., Corey D., Broeckling KR., Kirkpatrick JE., Penni., 2014. Application of Non Targeted Metabolite Profiling to Discover Novel Marker of Quality Traits in a Advanced Population of Marting Barley. Plant Biotechnology Journal. 12:147-160.

Id Climate Data, 8 Januari 2017. Data climate. www.id.climate-data.org

Katzung BG., 2007. Basic and Clinical Pharmacology 10th Edition. Jakarta: Penerbit Buku Kedokteran EGC.

Kim EJ., Kown J., Park SH., Park C., Seo YB., Shin HK., 2011. Metabolite Profillingof Angelica Gigas From Diferen Geographical OriginUsing H-NMR and UPLC-MS Analyses. 59: 8806-8815.

King RJ., Robins M., 2006. Cancer Biology Third Edition. England: Pearson Education Limited.

Kuntorini E., Astuti M., 2010. Penentuan Aktivitas Antioksidan Ekstrak Etanol Bulbus Bawang Dayak (Eleutherine americana Merr.). Sains dan Terapan Kimia. 4: 15-22.

Li, X, Ohtsuki T., Koyano T., Kowithayakorn T., Ishibashi M., 2008. New Wnt/β-Catenin Signaling Inhibitors Isolated from Eleutherine palmifolia. Chemistry An Asian Journal. 4: 540-547.

Maharani S., 2009. Mengenal 13 Jenis Kanker dan Pengobatannya. Jogjakarta: Katahati.

Mutiah R., Aty W., Sukardiman S., 2017. Ethyl Acetate Fraction Of Calotropis Gigantea Roots Induce Apoptosis Through Increased G2/M And Increased Expression Of Caspase-8 In Colon Cancer Widr Cell Line. Journal Of Applied Pharmaceutical Science. 7: 197-201.

Mutiah R., Aty W., Sukardiman S., 2018. Calotropis gigantea Leaf Extract Increases the Efficacy of 5-Fluorouracil and Decreases the Efficacy of Doxorubicin in Widr Colon Cancer Cell Culture. Journal of Applied Pharmaceutical Science. 8: 051-056.

Nagara FS., R Utomo A., Sandra F., 2008. Efek Mutasi K-Ras pada Kanker Kolorektal Terhadap Terapi Antibodi Monoklonal Anti EGFR. Indonesian Journal of Cancer. 4: 144-148.

Patel NG, Patel KG, Patel KV, Gandhi TR. 2015. Validated HPTLC Method for Quantification of Luteolin and Apigenin in Premna Mucronata Roxb., Verbenaceae. Hindawi. 2015: 1-7.

Prayong P., Barusrux S., Weerapreeyakul N., 2008. Cytotoxic Activity Screening of Some Indigenous Thai Plants. Fitoterapia. 79: 598-601.

Ravichandran V., S Shalini., M Sudram K., Rajak H., 2010. Validation of Analytical Methods-Strategies & Importance. International Journal of Pharmacy and Pharmaceutical Sciences. 2: 18–22.

Ren W., Qiao Z., Wang H., Zhu L., Zhan L., 2003. Flavonoids: Promising Anticancer Agents. 23: 519-534.

Sutejo IR., Putri H., Meiyanto E., 2016. The Selectivity of Ethanolic Extract of Buah Makassar (Brucea javanica) on In Vitro Study of Metastatic Breast Cancer. Journal of Agromedicine and Medical Sciences. 2.

Taufik I., 2017, Profil Metabolit Kulit Batang Artocarpus champeden Spreng Secara HPTLC Densitometri serta Hubungannya dengan Antimalaria dan Toksisitas in vito, Tesis, Program Studi Magister Ilmu Farmasi Universitas Airlangga, Surabaya.

Verma N., Shukla S., 2015. Impact of Various Factors Responsible for Fluctuation in Plant Secondary Metabolites. Journal of Applied Research on Medicinal and Aromatic Plants 2: 105–13.

Wolfender JL., Marti G., Thomas A., Bertrand S., 2015. Current Approaches and Challenges for the Metabolite Profiling of Complex Natural Extracts. Journal of Chromatography A 1382:136–64.

Yuwono M., Indriyanto G. 2005. Validation of Chromatograhic Method of Analysis. Profiles of Drug Subtances, Excipients and Related Methofology. 32: 243-259.

WHO, W.H. 2013. WHO Guidance Note: Comprehensive Colorectal Cancer Prevention and Control. Geneva: WHO Press.




DOI: http://dx.doi.org/10.14499/indonesianjpharm30iss3pp157

Refbacks

  • There are currently no refbacks.




Copyright (c) 2019 Indonesian Journal of Pharmacy

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian J Pharm indexed by:

web
analytics View My Stats