Free radical scavenging potential of Drosera indica L in presence of Dalton Ascites lymphoma (DAL) tumor bearing mice

Raju Asirvatam, AJM Christina


The aim of the present study was to evaluate the antioxidant role of Drosera indica L. in Dalton Ascites lymphoma (DAL) bearing Swiss albino mice. The effect of ethanol and aqueous extracts of D.indica (EEDI and AEDI) were administered at a 250 and 500mg/kg once a day for 14 days, 24h after the inoculation of tumor cell line. After the treatment period, blood was collected from the animals and subsequently the animal were sacrificed for isolation of liver, brain, kidney and lungs for the observation of antioxidant status level. The parameters analyzed were catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), peroxidase (P), total protein (TP) and protein carbonyl content (PCC). Treatment with EEDI and AEDI significantly reduced the levels of MDA and increased the levels of GSH, SOD, CAT, P and TP in cancer induced animal and are similar as that of normal mice. The results suggest that the ethanol and aqueous extract of Drosera indica L. possess significant antioxidant effects in DAL bearing mice


: Drosera indica L, Antioxidant, Dalton Ascites lymphoma, Malondialdehyde, Protein carbonyl content

Full Text:



Aebi H., 1974. Catalase. In: Bergmeyer, editor. Mehtods in enzymatic analysis, Vol.2, New York, Academic Press, pp 674-684.

Alexander NM., 1962. A spectrophotometric assay for Iodide Oxidation by thyroid peroxidase. Anal Biochem. 4: 341-345.

Angel L., Ortega., Salvador Mena., Jose M Estrela., 2011. Glutathione in Cancer Cell Death. Cancers. 3: 1285-1310.

Anil U Tatiya., Pallavi M Puranik., Sanjay J Surana., Yogesh S Patil., Rakesh E Mutha., 2013. Evaluation of hypolipidemic, antidiabetic and antioxidant activity of Eulophia herbacea tubers. Bangladesh J Pharmacol. 8: 269-275.

Anita Mukthapura., Avinash Sidhuraju Shimogga., Vinodchandran Sudha K., Beena V Shetty., Gayathri M Rao., 2010. Oxidative products of proteins and antioxidant potential of thiols in gastric carcinoma patients. Journal of Medical Biochemistry. 29: 102–106.

Balendiran GK., Dabur R., Fraser D., 2004. The role of glutathione in cancer. Cell Biochem Funct. 22(6):343-52.

Battisti V., Maders LDK., Bagatini MD., Santos KF., Spanevello RM., Maldonado PA., Brulé AO., Araújo M., Schetinger MRC., Morsch VM., 2008. Measurement of oxidative stress and antioxidant status in acute lymphoblastic leukaemia patients. J Clin Biochem. 41:511-518.

Beutler E., Kelly BM., 1963. The effect of sodium nitrate on red cell glutathione. Experientia. 18: 96-97.

Catherine W., Hosni MH., 1987. Introduction and inactivation of catalase and superoxide dismutase of Escherichia coli by ozone. Arch Biochem Biophy. 259(2):464-471.

Dalle-Donne I., Giustarini D., Colombo R., Rossi R., Milzani A., 2003. Protein carbonylation in human diseases. Tren Mol Med. 9:169-75.

Fatima M Nathan., Vivek A Singh., Amreeta Dhanoa., Uma D Palanisamy., 2011. Oxidative stress and antioxidant status in primary bone and soft tissue sarcoma. BMC Cancer. 11: 382-389.

Jones GM., Sanford KK., Parshad R., Gantt R., Price FM., Tarone RE., 1985. Influence of added catalase on chromosomal stability and neoplastic transformation of mouse cells in culture. Br J Cancer. 52: 583-90.

Kakkar P., Dos B., Viswanathan PN., Maehly AC., Chance B., 1954. In: Methods of Biochemical Analysis Vol. I, Glick D,editors. New York, Interscience, pp 357.

Kumaraguruparan R., Subapriya R., Viswanathan P., Nagini S., 2002. Tissue lipid peroxidation and antioxidant status in patients with adenocarcinoma of the breast. Clin Chim Acta. 325:165-70.

Levine RL., Williams JA., Stadtman ER., Shacter E., 1994. Carbonyl assays for determination of oxidatively modified proteins. Methods Enzymol. 233: 357-63.

Lowry OH., Rosebrough NJ., Farr AL., Randall RJ., 1951. Protein measurement with Folinciocal phenol reagent. J Bio Chem. 93(1): 265-276.

Morabito F., Cristani M., Saija A., Stelitano C., Callea V., Tomaino A., Minciullo PL., Gangemi S., 2004. Lipid peroxidation and protein oxidation in patients affected by Hodgkin’s lymphoma. Mediators Inflamm. 13: 381–383.

Niedernhofer LJ., Daniels JS., Rouzer CA., Greene RE., Marnett LJ., 2003. Malondialdehyde, a product of lipid peroxidation, is mutagenic in human cells. J Biol Chem. 278: 31426–33.

Ohkawa H., Onishi., Yagi K., 1979. Assay for lipid peroxidation in animal tissue by thiobarbituric acid reaction. Anal Bio Chem. 95: 351-358.

Raju A., AJM Christina., 2013d. Drosera burmannii Vahl: Antioxidant Potential in Dalton’s Ascites Lymphoma (DAL) Bearing Mice. Journal of Medicinal Plants Studies. 1(4): 151-159.

Raju A., Christina AJM., Anita Murali., 2013c. Antioxidant potential of Drosera burmannii Vahl. (Droseraceae) in Ehrlich Ascites Carcinoma (EAC) cell line inoculated Swiss albino mice. International Journal of Chemical And Pharmaceutical Research. 2(1): 015-027.

Raju Asirvatham., Arockiasamy Josphin Maria Christina., Anita Murali., 2013b. In Vitro Antioxidant and anticancer activity studies on Drosera Indica L. (Droseraceae). Advanced Pharmaceutical Bulletin. 3(1):115-120.

Raju Asirvatham., Arockiasamy Josphin Maria Christina., 2013a. Anticancer activity of Drosera indica L., on Dalton’s lymphoma ascites (DLA) bearing mice. Journal of Intercultural Ethnopharmacology. 2(1): 9-14.

Raju Asirvatham., Joshila Akhil et al., 2017.

Evaluation of in vitro and in vivo anti-

oxidant potential of Morinda reticulata GAMBLE tubers in Wistar albino rats subjected to CCl4 and paracetamol induced hepatotoxicity. Indonesian J. Pharm. 28 (3): 147 – 157.

Ray G., Batra S., Shukla NK., Deo S., Raina V., Ashok S., Husain SA., 2000. Lipid peroxidation, free radical production and antioxidant status in breast cancer. Breast Cancer Res Treat. 59(2):163-70.

Reddy CHS., Reddy KN., Jadhav SN., 2001. Threatened (Medicinal) plants of Andhra Predesh. Medicinal Plants Conservation Center. 1-39.

Rizwan Ahmad., Anil Kumar Tripathi., Payal Tripathi., Sushma Singh., Ranjana Singh., Raj Kumar Singh., 2008. Malondialdehyde and Protein Carbonyl as Biomarkers for Oxidative Stress and Disease Progression in Patients with Chronic Myeloid Leukemia. In vivo. 22: 525-528.

Sapan CV., Lundblad RL., Price NC., 1999.Colorimetric protein assay techniques. Biotech Appl Biochem. 29: 99-108.

Sara Jelodarian., Abdolrasoul Haghir Ebrahimabadi., Ahmad Khalighi., Hossain Batooli., 2012. Evaluation of antioxidant activity of Malus domestica fruit extract from Kashan area. Avicenna Journal of Phytomedicine. 2 (3):139-145.

Senem D., Zeynep O., Handan AKC., Arif BA., Hikmet HA., 2011. The interaction between antioxidant status and cervical cancer: a case control study. Tumori. 97:290-295.

Sinha RJ., Singh R., Mehrotra S., Singh RK., 2009. Implications of free radicals and antioxidant levels in carcinoma of the breast: A never-ending battle for survival. Indian J Cancer. 46:146-50.



  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian J Pharm indexed by:

analytics View My Stats