Isolation and Identification of α-Glucosidase Inhibitor From Aspergillus Terreus F38

Siti Munasaroh; Swasono R Tamat; Rizna Triana Dewi

doi:10.14499/indonesianjpharm29iss2pp74

Isolation and Identification of α-Glucosidase Inhibitor From Aspergillus Terreus F38

Siti Munasaroh, Swasono R Tamat, Rizna Triana Dewi

Abstract

Controlled postprandial glucose level is an important strategy in preventing DM type 2. Inhibitors of α-glucosidase have been postulated to be useful agents in managements of DM type 2. This research aims to isolate and identify of α-glucosidase inhibitor fromAspergillus terreus F38 by liquid fermentation. The mycelium extract of A. terreus F38showed strong activity against α-glucosidase with IC₅₀value of 9.65 μg/mL. Separation and purification of mycelium ectract yielded compound I (Butyrolactone III). The structure was establish on the basis of spectral analysis, according to the data obtained by NMR and LCMS-MS experiments. Compound I showed potential activity against α-glucosidase with IC₅₀value of 13.87 μg/mL. Therefore, the metabolites from A. terreus F38 can be used as lead compound to design potent α-glucosidase inhibitory agents.

Keywords

α-glukosidase inhibition, Aspergillus terreus F38, butyrolactone III.

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DOI: http://dx.doi.org/10.14499/indonesianjpharm29iss2pp74