The Antihyperglicemic Activity of ethanol Extract of Fenugreek seed (Trigonella foenum graecum L) and its effect on the GLUT-2 expression of Streptozotocin Nicotinamide Induced Rats

Jena Hayu Widyasti, Gunawan Pamudji Widodo, Rina Herowati


Insulin is an important factor in glucose transport. Glucose transporter (GLUT-2) in pancreatic β cell membranes is responsible for glucose transport that will stimulate insulin secretion. The purpose of this research was to investigate the antihyperglycemic activities of extract of Trigonella foenum-graecum L. (fenugreek) seed and the effects on GLUT-2 protein expression in pancreatic β cells on Streptozotocin-Nicotinamide-induced (STZ-NA) rats. This research utilized five groups of male Wistar rats. Group I was normal control group, while Groups II-V were induced with STZ-NA. Group II was negative control group (CMC Na 1%); group III was positive control group (Glibenclamide); group IV and V was examined group (fenugreek seed ethanol extract of 100mg/kg bw and 200 mg/kg bw)). The test compounds were orally administered for 14 days. Measurement of blood glucose levels and observation of the expression of GLUT-2 protein in pancreatic β cells in rats were conducted by immunohistochemistrical method. The research results revealed that the examined group dose of 200 mg/kg bw of fenugreek seed extract appeared to show blood glucose level decline that was proportionate to that of the positive control group. The increased density of GLUT-2 protein in pancreatic β cells significantly occurred in the group injected with 200 mg/kg bw compared to the group injected with 100 mg/kg bw. Hence, 200 mg/kg bw of fenugreek seed ethanol extract was able to decrease blood glucose levels and increase the GLUT-2 protein expression in pancreatic β cells in STZ-NA-induced rats with the effectiveness that was comparable to the positive control group


Trigonella foenum graecum L, streptozotocin nicotinamide, Glucose transporter-2

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Baquer NZ., P Kumar A., Taha RK., et al, 2011. Metabolic and Molecular Action of Trigonella Foenum Graecum (Fenugreek) and Tree Metals in Experimental Diabetes Tissues. J Biosci. 36: 383-396.

Basch E., Ulbricht C., Kuo G., et al, 2003. Therapeutic Applications of Fenugreek. Altern Med Rev. 8(1): 20-27.

Broca C., Breil V., Cruciani-Guglielmacci, C., et al, 2004. Insulinotropic Agent ID-1101 (4- Hydroxyisoleucine) Activates Insulin Signaling In Rat. Am J Physiol Endocrinol Metab. 287(3):. 463-471.

Cavallerano JOD Ph.D., 2009. Care of The Patient with Diabetes Mellitus.

Ferrer J., Benito C., Gomis R., 1995. Pancreatic Islet GLUT2 Glucose Transporter Mrna and Protein Exspression in Humans With and Without NIDDM. Diabetes. 44: 1369-1374.

Gupta A., Gupta R., Lal B., 2001. Effect of Trigonella Foenum-Graecum (Fenugreek) Seeds on Glycaemic Control and Insulin Resistance in Type 2 Diabetes Mellitus: A Double Blind Placebo Controlled Study. J Assoc Physicians India. 49: 1057-1061.

Henquin JCMA., Ravier M., Nenquin JC., et al, 2003. Hierarchy of the Beta-Cell Signals Controling Insulin Secretion. European Journal of Clinical Investigation. 33(9):742-750.

Kulkarni CP., Bodhankar SR., Gule, AE., et al. 2012. Antidiabetic Activity Of Trigonella foenum graecum L. Seeds Extract (Ind01) in Neonatal Streptozotocin-Induced (N-Stz) Rats. Diabetologia Croatica. 41-1: 29-40.

Qi LW., Liu EH., Chu C., et al. 2010. Antidiabetic Agents from Natural Products. Curr Top Med Chem. 10(4): 434-457.

Raju J., Gupta D., Rao AR., et al, 2001. Trigonella foenum graecum (Fenugreek) Seed Powder Improves Glucose Homeostasis in Alloxan Diabetic Rat Tissues By Reversing the Altered Glycolytic, Gluconeogenic And Lipogenic Enzymes. Mol Cell Biochem. 224(1-2):45-51.

Xiong X., Wang X., Li B., et al, 2011. Pancreatic Islet-Specific Overexpression of Reg3_ Protein Induced the Expression of Pro-Islet Genes and Protected the Mice Against Streptozotocin Induced Diabetes Mellitus. Am J Physiol Endocrinol Metab 300: E669–E680.



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