2024-03-29T06:12:46Z
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/oai
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1691
2021-01-06T13:13:53Z
3:ART
An Assessment of Cough Medicine Dispensing Practice to Children Under Two Years Old in Pharmacies in Ho Chi Minh City Using Simulated-Patient Method
Phan, Quyen Thi Ngoc; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Truong, Van Thi; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Pham, Thanh Ngoc; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Nguyen, Ho Nhu; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Department of Pharmacy, Nguyen Trai Hospital, Vietnam
Bui, Quynh Thi Huong; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Department of Pharmacy, Thong Nhat Hospital, Vietnam
Cough medications, Children, Pharmacy
Over-the-counter (OTC) cough and cold medications (CCMs) have been used to treat the symptoms of upper respiratory infection in children for decades. The safety of CCMs in children has been questioned. The data on knowledge of pharmacists in supplying cough medicines for children under two years have been limited. This study aimed to evaluate the pharmacists’ dispensing decisions to manage the cough in children under two years old. A descriptive cross-sectional was carried out in 300 pharmacies in 15 districts in Ho Chi Minh City, Vietnam. The pharmacists were interviewed by a simulated patient. The results showed that, information that pharmacists actively asked the client about the patient and disease symptoms was limited. Most pharmacists did not provide adequate instructions and counsel about using drugs for clients. Only 22/300 (7.33%) of pharmacists appropriately provided cough medicines for children under 2 years old. The main reason of inappropriateness was the deficiency of knowledge about updated contraindication of N-acetylcysteine (93.17%). Pharmacists in pharmacies located in districts 3, 11 and Binh Thanh had higher rate of rational provision than those in other districts. A good and full understanding of the patient symptom helped the pharmacists supply cough medicines more reasonably. The limited caution of pharmacists and the low proportion of pharmacists updating contraindication of N-acetylcysteine should be considered as a warning sign in pharmacy practice in Ho Chi Minh City, Vietnam.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-04-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1691
10.14499/indonesianjpharm31iss1pp42
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 42
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1691/943
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1691/265
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/517
2018-09-05T01:49:55Z
3:ART
ANTIHEPATOTOXIC ACTIVITY OF TWO NEW QUERCETIN DERIVATIVES IN CARBON TETRACHLORIDE INDUCED HEPATOXICITY IN RATS
Khan, Shah Alam; Department of Pharmacy, Oman Medical College, Muscat, Sultanate of Oman PO Box 82, Ruwi, PC 112 Oman
Ahmed, Bahar; Antihepatotoxic Research Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy Jamia Hamdard, New Delhi110062, India
Quercetin, a bioflavonol is widely found in nature and possesses diverse pharmacological properties. A heterocyclic 1,4 dioxane nucleus was incorporated in Quercetin structure to obtain two structural analogues of silybin. The aim of the study was to antihepatotoxic potential of Quercetin derivatives containing 1,4 dioxane heterocyclic ring in Carbon tetrachloride (CCl4) induced hepatotoxicity in female Wistar Albino rats. Two Quercetin derivatives (QD) were synthesized by reported method. QD were administered orally at dose of 10 mg/kg, once daily for 7 days to Wistar Albino rats. A single dose of CCl4(1mL/kg) was used for inducing liver damage. Antihepatotoxic activity was evaluated by measuring levels of total proteins (TP), total albumin (TA), alkaline phosphatase (ALKP) and liver enzymes such as serum glutamate oxaloacetate (SGOT) and serum glutamate pyruvate transaminase (SGPT).QD exhibited potent antihepatotoxic activity with respect to standard drug silybon-70. However, it was observed that the Quercetin derivative having –CH2OH group in the dioxane ring exhibited better activity in comparision to unsubstituted 1,4 dixoane ring derivative.The exact mechanism by which QD protects the liver is unknown however the observed effects could be attributed to presence of 1,4 dioxane ring and due to the significant antioxidant activity of Quercetin flavone. Key words: Antihepatotoxic activity, Quercetin; Silymarin, 1,4 dioxanering, CCl
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2013-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/517
10.14499/indonesianjpharm24iss1pp56-60
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 56-60
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/517/395
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1308
2018-10-23T02:19:33Z
3:ART
Protective Effect of Ethanolic Extract Tempuyung Leaf (Sonchus arvensis L.) in Gentamicin-induced Acute Tubular Necrosis on Wistar Rats
Imelda, Imelda -; Biomedical Science Master Program, Graduate School of Medical Sciences, Universitas Padjadjaran, Bandung
Achadiyani, Achadiyani -; Department of Anatomy, Physiology and Cell Biology,Faculty of Medicine, Universitas Padjadjaran, Bandung
Sekarwana, Nanan -; Department of Pediatrics, Faculty of Medicine, UNPAD-RSHS, Bandung
acute tubular necrosis, creatinine, gentamicin, tempuyung, Sonchus arvensis L.
Acute tubular necrosis (ATN) is the most common histopathologic findings of acute kidney injury (AKI). AKI is marked by the decrement of glomerular filtration rate, causing waste metabolism retention (creatinine). Gentamicin is used as it is the most common nephrotoxic agent in inducing ATN. Tempuyung (Sonchus arvensis L.) has been used in folklore medicine to ameliorate kidney problems as it contains antioxidants, two of which are flavonoid and phenolic acid. Yet, these active substances’ benefit on gentamicin induced ATN has not been investigated in Indonesia. This research aims to analyze protective effect of ethanolic extract tempuyung leaf (EET) on gentamicin-induced ATN based on histopathological study and creatinine serum level. True experimental laboratoric study was done with simple random design on male wistar rats, randomly divided into 4 groups (n=4). Control group (CMC 0.5% aquadest); Induction group (Gentamicin 80 mg/kgBW); 1st treatment group (EET 100 mg/kgBW + Gentamicin 80 mg/kgBW) and 2nd treatment group (EET 200 mg/kgBW + Gentamicin 80 mg/kgBW) for ten days. On the 11th day, blood was taken for creatinine measurement and kidneys collection for histopathological study. Histopathological examination on gentamicin-treated rats revealed degenerative changes in kidney tubules. Aside from that, gentamicin-treated rats also showed increment in creatinine serum level. Conversely, simultaneous administration of EET with Gentamicin ameliorated the nephrotoxic effects of gentamicin as confirmed from the significant improvement on histopathological changes and normalization of creatinine serum level. Co-administration of EET and gentamicin provides protection on gentamicin-induced ATN, based on histopathological feature and creatinine serum level.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
-
2018-05-11
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1308
10.14499/indonesianjpharm29iss2pp86
Indonesian Journal of Pharmacy; Vol 29 No 2, 2018; 86
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1308/870
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1308/142
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1308/143
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1308/144
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1439
2019-10-10T01:30:23Z
3:ART
Immunomodulatory Activity of Yogurt Fortified with Honey and Rosella (Hibiscus sabdariffa L.) on Reactive Oxygen Intermediate (ROI) and Nitric Oxide (NO) Secretion
Mahfudh, Nurkhasanah; Faculty of Pharmacy, Universitas Ahmad Dahlan
Novitasari, Rina; Faculty of Pharmacy, Universitas Ahmad Dahlan
Immunomodulator, Yoghurt, Rosella (Hibiscus sabdariffa L), ROI, NO
Raise the body's immune system. Rosella (Hibiscus sabdariffa Linn.) is known to have anthocyanin compounds that have antioxidant and immunomodulatory effects. The aim of this research is to know the effect of immunomodulator yogurt which fortified by rosella (Hibiscus sabdariffa L.) extract to increase the secretion of Reactive Oxygen Intermediate (ROI) and Nitric Oxide (NO). This research was conducted in vivo using 25 male mices with Balb/C strain divided into 5 groups: normal group, plain yogurt treated group and 2%, 4%, 8% rosella yogurt treated group. Treatment was given for 21 days orally. On the 22nd day the mice were sacrificed by taking peritoneum macrophage cells and then tested the secretion of Reactive Oxygen Intermediate (ROI) and Nitrite Oxide (NO). The results showed that there was an increase of ROI and NO secretion in 2%, 4%, and 8% rosella yoghurt treated groups compare to plain yoghurt. The fortification of yoghurt with rosella extract and honey increase the potency of yoghurt in increasing the immunomodulatory activity
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-05-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1439
10.14499/indonesianjpharm30iss2pp141-146
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 141-146
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1439/901
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/677
2020-01-27T02:53:16Z
3:ART
ELECTROCHEMICAL ANALYSIS OF ZINECARD IN PHARMACEUTICAL AND BIOLOGICAL SAMPLES
Reddy, Chennupalle Nageswara; Electroanalytical lab, Department of Chemistry,
Tirupati – 517 502, A.P., India
Prasad, Puthalapattu Reddy; Department of Chemistry, Govt. Degree College, Koduru, A.P.India
Sreedhar, Neelam Yugandhar; Electroanalytical lab, Department of Chemistry,
Tirupati – 517 502, A.P., India
Zinecard is used to prevent a toxic effect to heart caused by certain medicines that are used to treat cancer. Zinecard is also used to treat tissue damage caused by the leakage of certain medicines that are used to treat cancer. Differential pulse polarographic method developed for the quantitative determination of zinecard gives a peak at -0.32 V at DME. From the structural point of view zinecard contains a >C=Omoiety which can be electrochemically reduced at universal buffer (pH 4.0). Millicoulometric experiment is performed successfully in estimating the number of electrons and proton to understand reduction mechanism. The differential pulse polarographic peak was adequately well-resolved, reproducible and linear dependent with the zinecard concentration. For quantification the calibration plot for zinecard concentrations ranging between 1.0×10-5mol dm-3 to 1.0×10-8mol dm-3 at pH 4.0 was selected. The proposed differential pulse polarographic method was successfully applied to the determination of zinecard in pharmaceutical formulations and urine samples.Key words: Zinecard, DPP, pharmaceutical formulations and urine samples.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/677
10.14499/indonesianjpharm23iss3pp147-155
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 147-155
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/677/543
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1492
2022-10-26T01:05:53Z
3:ART
Quantitative Analysis of Multi-components in Curcuma xanthorrhiza by Single Marker
Badrunanto, Badrunanto
Wahyuni, Wulan Tri
Nirwati, Hera
Rafi, Mohamad
C. xanthorrhiza; curcuminoids; QAMS; relative coefficient factor
A new simple and effective routine analytical method for quantification of curcuminoids in Curcuma xanthorrhiza was developed by high-performance liquid chromatography. This method based on chromatographic fingerprint combined with a quantitative analysis of multi-components by single marker (QAMS). Curcumin was selected as an internal marker for the determination of two other similar compounds, i.e. bisdemethoxycurcumin and demethoxycurcumin, by using the relative coefficient factor (RCF). Excellent linearity was obtained for each component (r2 > 0.9998), and the recovery of extraction methods were within 100.23-103.95%. The precision of the method was good at inter-day and intra-day analysis (RSD < 4.0%). The stability of RCFs was good under various chromatographic conditions with RSD < 1%, and the ratio of retention time was used to locate each compound. The quantification of curcuminoids between QAMS and external standard method (ESM) proved the consistency and similarity of the two method (RSD < 2%). This study demonstrated that QAMS could be used as a routine method for quality control of curcuminoids in C. xanthorrhiza. This method successfully proved accurate, stable, more effective and simple than external standard method.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-10-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1492
10.14499/indonesianjpharm30iss4pp301
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 301
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1492/921
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/656
2020-01-27T02:53:58Z
3:ART
APPLICATION OF FOURIER TRANSFORM INFRARED SPECTROSCOPY FOR QUALITY CONTROL OF PHARMACEUTICAL PRODUCTS: A REVIEW
Rahman, Abdul; Laboratory of Analytical Chemistry, Faculty of
Pharmacy, Universitas Gadjah Mada, 55281,
Yogyakarta, Indonesia. +62274-543120
Analysis of pharmaceutical products covers all aspects of quality control of active pharmaceutical ingredients (API) and finished products. Today, Fourier transform infrared (FTIR)spectroscopy, especially in combination with chemometrics software, has emerged as one of the romising analytical techniques to be used in pharmaceutical industry, for quality control of desired pharmaceutical products. Compared with other instrumental techniques, FTIR spectroscopy offers some advantages, namely it is rapid, simple in sample preparation, and not destructive. In this review, the application of FTIR spectroscopy for qualitative and quantitative determinations of API and monitoring drug release are described.Key words: FTIR spectroscopy, quality control, pharmaceutical products.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/656
10.14499/indonesianjpharm23iss1pp1-8
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 1-8
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/656/522
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1235
2018-10-23T02:19:33Z
3:ART
Antimicrobial activity and Identification of fungus associated Stylissa flabelliformis sponge collected from Menjangan Island West Bali National Park, Indonesia
Setyowati, Erna Prawita; Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada
Pratiwi, Silvia Utami Tunjung; Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada
Purwantiningsih, Purwantiningsih; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Universitas Gadjah Mada
Samara, Oka; Faculty of Pharmacy, Udayana University
Sponge-associated fungi, Stylissa flabelliformis, Staphylococcus aureus, Escherichia coli, Candida albicans
The Fungus is a very important microorganism as a producer of bioactive secondary metabolites. Active substances of microbial origin have been sought through the process of screening methods to obtain antimicrobial compounds. The purpose of this study was to isolate fungi associated with sponge taken from Menjangan Island National Park West Bali (Indonesia) and identify fungi that have antimicrobial activity. Isolation of fungus from sponge was carried out by spread plate method using Saboroud Saline Agar medium. Each fungi will be tested to Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922 and Candida albicans ATCC 10231. Identification of fungi is based on the observation of macroscopic, microscopic and also using 16rRNA/ITS phylogeny tree. The results showed that S. flabelliformis sponge had 10 fungal isolates. Most of them have antimicrobial activity. The name associated with a sponge fungus is These 10 fungus are Aspergillus flavus strain UPMZ02, Aspergillus fumigatus strain CD1621, Trichoderma reesei strain JCM 2267, Aspergillus nomius strain KUB105, Aspergillus sp. strain TLWK-09, Aspergillus flavus strain MC-10-L, Penicillium sp. strain RMA-2, Aspergillus sp. strain TLWK-09, Aspergillus fumigatus and Trichoderma reesei strain TV221
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-11-27
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1235
10.14499/indonesianjpharm29iss2pp66
Indonesian Journal of Pharmacy; Vol 29 No 2, 2018; 66
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1235/847
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1425
2019-10-03T03:16:50Z
3:ART
Efficacy of Thymol and Eugenol Against Polymicrobial Biofilm
Hamzah, Hasyrul; Faculty of Pharmacy, Universitas Gadjah Mada
Tunjung Pratiwi, Sylvia Utami
Hertiani, Triana; Faculty of Pharmacy, Universitas Gadjah Mada
Thymol, Eugenol, polymicrobial biofilm, MBIC50, MBEC50
Biofilms associated with human infection have high levels of pathogenicity due to their resistance to antibiotics. The discovery of an active antibiofilm agent against polymicrobial biofilms is a necessary consequence for coping with biofilm-related infections. Thymol and Eugenol are essential oils that have potential as antibacterial and antifungal. This study aimed to determine the effectiveness of thymol and eugenol inhibits C. albicans, P. Aeruginosa, E. coli S. aureus and polymicrobial biofilm. Biofilm formation inhibition assay and biofilm degradation assay of thymol and eugenol were determined using microtiter broth method.The antibiofilm efficacy of thymol and eugenol towards polymicrobial biofilms were analyzed by calculating minimum biofilm inhibitor concentration (MBIC50) and minimum biofilm eradication concentration (MBEC50) values. The data were analyzed using Statistical Package for the Social Sciences (SPSS) with 95% confidence level. Thymol and eugenol showed inhibitory activity against the formation of mono and polymicrobial biofilms of the microbial tested.The result also demonstrated an evidence of activity of thymol and eugenol in breaking down mono and polymicrobial biofilm. Therefore, thymol and eugenol serves as a potential source for new antibiofilm drugs towards polymicrobial biofilm.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-12-17
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1425
10.14499/indonesianjpharm29iss4pp214
Indonesian Journal of Pharmacy; Vol 29 No 4, 2018; 214
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1425/886
Copyright (c) 2018 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1529
2021-01-06T13:13:53Z
3:ART
Inhibition of Cell Cycle and Induction of Apoptosis y Ethanol Leaves Extract of Chrysanthemum cinerariifolium (Trev.) In T47D Breast Cancer Cells
Mutiah, Roihatul; Department of Pharmacy, Faculty of Medicine and Health Science, Maulana Malik Ibrahim Islamic State University of Malang
Inayatin, Alfiyah Laily; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
Annisa, Rahmi; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
Indrawijaya, Yen Yen Ari; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
Listiyana, Anik; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
C. cinerariifolium, apoptosis, cell cycle, T47D cells
Chrysanthemum cinerariifolium (C.cinerariifolium) is a plant of the Asteraceae family, which has been applied by the community as an ornamental plant and traditional medicine. In this study, the effect of C. cinerariifolium leaves extract on inhibition of cell cycle and induction of apoptosis in T47D breast cancer cells was tested and compared to the standard chemotherapy agent. The citotoxic activity of C. cinerariifolium leaves extract against T47D cancer cells and Vero normal cells was tested by MTT method. Profile of apoptosis and cell cycle were observed by flow cytometry method. Based on chemical compounds profil which is tested used TLC showed that C.cinerariifolium leaves extracts contained flavonoid and terpenoid chemical compounds. The result of cytotoxic test showed that leaves extract of C. cinerariifolium was able to inhibit the growth of T47D cancer cell at IC50 418.8μg/mL. Doxorubicin, extracted from Streptomyces peucetius used as treatment in several cancers including breast cancer. Doxorubicin could inhibit the growth of T47D cancer cells in 115.1μg/mL. The results of cell cycle analysis showed that the C. cinerariifolium leaves extract inhibited cell cycle in G0-G1 and S phase, whereas doxorubicin was able to inhibit cell cycle in G0-G1 phase but experienced cell accumulation in G2-M phase. The percentage of apoptosis in cycle was showed in M1 (sub G1) and M5 (multinuclear) phase which treatment of C. cinerariifolium leaves extract was higher than doxorubicin. Therefore, C. cinerariifolium leaves extract has potential activity as anticancer agent causes inhibition of cell cycle and induction apoptosis.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
This work supported by The Directorate General of Islamic Higher Education (DIKTIS) of Interdisciplinary Basic Research Grant numbers 3209/Un.3/HK.00.5/05/2018.
2020-04-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1529
10.14499/indonesianjpharm31iss1pp1
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 1
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1529/937
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/511
2018-09-05T01:49:54Z
3:ART
DESIGN, DEVELOPMENT AND IN VITRO EVALUATION OF CAFFEINE LOADED NATURAL GUM MATRIX TABLET
Jain, Nidhi; Dept. of PharmaceuticsSchool of Pharmacy, Bharat Institute of Technology., By-Pass Road, Partapur, Meerut250103, Uttar Pradesh, India
Kumar, Divya; Dept. of PharmaceuticsSchool of Pharmacy, Bharat Institute of Technology., By-Pass Road, Partapur, Meerut250103, Uttar Pradesh, India
Gulati, Neha; Dept. of PharmaceuticsSchool of Pharmacy, Bharat Institute of Technology., By-Pass Road, Partapur, Meerut250103, Uttar Pradesh, India
Nagaich, Upendra; Dept. of PharmaceuticsSchool of Pharmacy, Bharat Institute of Technology., By-Pass Road, Partapur, Meerut250103, Uttar Pradesh, India
The present research work was carried out to develop sustained release tablets of caffeine using natural matrix former (tragacanth) and different filling polymers like hydroxyl propyl methyl cellulose (HPMC K15M) and ethyl cellulose (EC). Caffeine was used as model drug. The polymers and tragacanth gum were incorporated in varying ratios into a matrix system using wet granulation technique. All the lubricated formulations were compressed into tablets and evaluated for various physicochemical properties such as thickness, hardness, friability, weight variation, drug content and in vitro drug release studies. From the investigation it was observed that increase in the amount of gum tragacanth (from F1 to F5) led to reduced friability, increased hardness and retarded drug release. Different filling polymers also sustained the drug release. The in vitro drug release data were tted in various release kinetics models to understand th mechanism of drug release. All solid matrix formulations were found to follow Higuchi kinetics, indicating the diffusional release of drug from the matrix type system. The Formulation F5 containing highest amount of gum tragacanth have shown promising results. The findings of the current investigation clearly indicate the potential of tragacanth gum to be used as release retardant and natural matrix material in sustained release formulations. Key words: Sustained release, Matrix tablets, Tragacanth, Caffeine.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2013-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/511
10.14499/indonesianjpharm24iss1pp30-34
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 30-34
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/511/389
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1101
2018-10-23T02:19:20Z
3:ART
Formulation and Evaluation of Glipizide Tablets Utilizing Hibiscus Rosasinensis Leaves Mucilage
Bahadur, Sanjib; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Roy, Amit; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Baghel, Pragya; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Choudhury, Ananta; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Saha, Suman; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Chanda, Ranabir; Bengal School of Technology, Sugandha, Delhi Road, Hooghly, West Bengal, 712102
This research work aims to develop glipizide tablets using Hibiscus rosasinensis leaves mucilage. The mucilage was extracted by using double distilled water and precipitated with ethanol. The precipitated mucilage was dried and grounded into powder. The tablet utilizing the mucilage as excipient was prepared by wet granulation method. The tablets were subjected to various tests. The evaluatory parameters of tablets were found to be within the limits as per United States Pharmacopoeia NF 24/19. FTIR spectrum reveals that there is no incompatibility between the ingredients used. Diabetes was induced in wistar albino rats using streptozotocin and effect of formulation on blood glucose level was determined. It was observed that the formulation could not sustain the release of glipizide. However, the glipizide release was retarded as the amount of mucilage was increased. It was observed that on the completion of antidiabetic study, the formulation could bring the blood glucose level to normal in group rats. However, the blood glucose level was still elevated in group administered with pure gliplizide. It can be concluded that HRM can be used to formulate glipizide tablets. The formulations with HRM shows better hypoglycemic activity and this may be attributed to antidiabetic activity of Hibiscus rosasinensis.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-03-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1101
10.14499/indonesianjpharm29iss1pp23
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 23
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1101/860
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1512
2019-10-10T01:29:33Z
3:ART
Conjugation of Anti-EpCAM Antibody on Alginate–RIP MJ-30 Nanoparticle through Carbodiimide Reaction as a Model of Targeted Protein Therapy
Ismail, Hilda; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia – 55284
Ciptasari, Ummi H.; Undergraduate Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia - 55284
Ikhsan, M Arief Nur; Undergraduate Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia - 55284
Suryani, Fidya; Undergraduate Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia - 55284
Sismindari, Sismindari; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia – 55284
Martien, Ronny; Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia – 55284
Yuswanto, Ag; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia – 55284
Ribosome inactivating proteins from Mirabilis jalapa L. (RIP MJ) has shown higher cytotoxic activity when being formulated as a nanoparticle. However, the selectivity of the delivery system is also an important aspect when it comes to cytotoxic cell therapy. Epithelial cell adhesion molecule (EpCAM) is a monomeric glycoprotein which is overexpressed in epithelial cancer cells. This study aim was to develop a model of targeted protein delivery system by formulating the base fraction of RIP MJ (RIP MJ-30) into alginate nanoparticles and conjugating it with anti-EpCAM antibody. RIP MJ-30 was formulated in to nanoparticle using alginate and CaCl2 as cross-linker. Optimization of conjugation reaction condition was done in the pH variation of 4.5, 5.5, and 6.5. The success of conjugation was analyzed qualitatively using native polyacrylamide gel electrophoresis (native-PAGE) method and BCA assay. The optimum formula of RIP MJ-30 nanoparticles was produced using 0.3% alginate and 0.2% CaCl2. Results indicated that optimum conjugation reaction was carried out at pH level of 5.5. The optimum native-PAGE condition was by using 8% polyacrylamide gel in duration of 6h. Characterization of nanoparticle resulted in particle size of 205.0nm, zeta potential of -6.9mV, entrapment efficiency of 71.11±4.84%, and conjugation efficiency of 89.55±6.18%. It was concluded that RIP MJ-30 was successfully formulated into alginate nanoparticle and conjugated to anti-EpCAM antibody through carbodiimide reaction using 1-ethyl-(dimethylprophilamine) carbodiimide (EDAC).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-03-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1512
10.14499/indonesianjpharm30iss1pp52
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 52
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1512/893
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1257
2018-06-22T02:08:58Z
3:ART
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/671
2020-01-27T02:51:49Z
3:ART
INFLUENCE OF Morinda citrifolia, L. FRUIT EXTRACT AS ADJUVANT ON IG-Y PRODUCTION RAISED IN LAYING CHICKENS AGAINST AVIAN INFLUENZA VACCINE
Sasmito, Ediati; Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. Skip Utara Yogyakarta, 55281
Harimurti, Sri; Faculty of Animal Sciences, Universitas Gadjah Mada, Yogyakarta, Indonesia
Nurhidayat, Hendy; Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. Skip Utara Yogyakarta, 55281
Anggoro, Wahyu; Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. Skip Utara Yogyakarta, 55281
Hikmawati, Elika Nur; Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. Skip Utara Yogyakarta, 55281
Wibowo, Joko Tri; Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. Skip Utara Yogyakarta, 55281
Avi, Atut Sulma; Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. Skip Utara Yogyakarta, 55281
Hilmi, Nurul; Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. Skip Utara Yogyakarta, 55281
Immunized avian with vaccine, produces specific antibody in the serum as well as in the egg yolk. An adjuvant is used to augment the effects of a vaccine by stimulating the immune response. Morinda citrifolia, L., especially the fruit contains of some chemical compounds such as polysaccharide, scopoletin, damnacanthal, proxeronine and proxeroninase. All those of active compounds, are soluble in different solvents and have different activities. Some of those compounds have adjuvant activity. The aim of our study was to observe the capability of M.citrifolia fruit extracts as adjuvant to enhance the specific antibody (IgY) production in immunized laying chickens Lohmann strain (28 weeks old) with a commercially available avian influenza (H5N2) vaccine. To the groups (@6) of laying chickens were orally administered once a day, through out of the study, with n-hexane (group I; divided ino 3 sub groups), ethanolic (group II; divided into 3 subgroups) and aqueous extract (group III; divided into 3 subgroups) of M.citrifolia (prepared in capsules) on dose of 29 mg; 58 mg; and 116 mg/chicken/day, respectively, and group IV as plasebo control and group V as normal control. All of the groups were induced with avian influenza vaccine, except the normal control. Eggs of 3, 6, and 10 weeks after immunized, were collected. Isolation of IgY was performed by repeated polyethylene glycol 6000 precipitation steps. IgY obtained was analyzed by indirect ELISA method. The serum was tested for antibody specificity against the influenza H5N2 virus by hemagglutination-inhibition (HI) method. The result of this study showed that the dose of 58 mg/chicken/day of aqueous extract of M.citrifolia fruit had an optimal capability to enhance specific antibody (IgY) as well as IgG serum production against avian influenza (H5N2) vaccine .Key words: Adjuvant, extract, Morinda citrifolia, L., H5N2 vaccine, immunoglobulin yolk (IgY)
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/671
10.14499/indonesianjpharm23iss2pp115-120
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 115-120
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/671/881
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1545
2019-10-28T01:20:02Z
3:ART
Effect of 7-Hydroxy-2-(4- Hydroxy -3-Methoxyphenyl)-Chroman-4-one On Level of Mangan-Superoxide Dismutase (Mn-Sod) and Superoxide Dismutase 2 (Sod2) Gene Expression in Hyperlipidemia Rats
Ayunda, Rahmah Dara; Magister of Biomedical Science Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada
Prasetyastuti, Prasetyastuti; Departement of Biochemistry Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada
Hastuti, Pramudji; Department of Biochemistry Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada, Skip Utara 55281 Yogyakarta
Hyperlipidemia, flavonoids, Mn-SOD, SOD2 gene
Hyperlipidemia is a lipid metabolism disorder characterized by an increase in serum lipid levels. Hyperlipidemia is a major risk factor for many metabolic syndrome diseases because it triggers oxidative stress. Oxidative stress can be reduced by endogenous antioxidant enzymes triggered by exogenous antioxidant compounds, such as 7-OH-2- (4-OH-3-methoxyphenyl)-chroman-4-one isolated from the seeds of Swietenia macrophylla King. The aims of this study were to investigate the effects of 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one compounds on cholesterol level, LDL level, Mn-SOD levels and SOD2 gene expression of hyperlipidemic rats. Thirty rats (Rattus norvegicus ) were divided into 6 groups, normal group (N), hyperlipidemia group (HL), hyperlipidemia group with simvastatin (P), hyperlipidemic group with 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one with dose 10 (F10), 30 (F30) and 90 (F90) mg/200g body weight (BW). Cholesterol and LDL were analyzed with CHOD-PAP method, Mn-SOD level was analyzed by ELISA method and SOD2 gene expression was analyzed by qPCR method. The decrease in cholesterol and LDL levels were most prevalent in group F90 with dose 90 mg/200g BW of 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one, with average difference each of them was 172.43 mg/dL and 36.12 mg/dL. The rats fed on high-cholesterol diet exhibited a significant elevation in Mn-SOD levels (p<0.05) compared to normal group. The treated animals with 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one has the level of Mn-SOD is significantly lower (p<0.05) compared with hyperlipidemic group. Expression of SOD2 in group F90 has value close to normal group (p> 0.05). 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one with dose of 90 mg/200g BW improved cholesterol levels, LDL levels, Mn-SOD levels and SOD2 gene expression in hyperlipidemic rats.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-08-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1545
10.14499/indonesianjpharm30iss3pp180
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 180
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1545/915
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/660
2020-01-27T02:53:58Z
3:ART
ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF RED Psidium guajavaL. LEAVES GROWN IN MANOKO, LEMBANG - INDONESIA
Fidrianny, Irda; Departement of Biology Pharmacy, School of Pharmacy, Intitut Teknologi Bandung
Jl. Ganesha 10 Bandung-40132 Indonesia
Hartanti, Rika; Departement of Biology Pharmacy, School of Pharmacy, Intitut Teknologi Bandung
Jl. Ganesha 10 Bandung-40132 Indonesia
Raveendaran, Narmmatha; Departement of Biology Pharmacy, School of Pharmacy, Intitut Teknologi Bandung
Jl. Ganesha 10 Bandung-40132 Indonesia
Psidium guajava L. (Myrtaceae) is a well known plant in Malaysia and Indonesia. Its leaves extract was found to possess antidiarrhea, antimicrobial, hepatoprotective and antioxidant activities. Objective of this research is to isolate an antioxidant subtance from Red Psidium guajava L. leaves. The crude leaves was extracted using Soxhlet apparatus by gradual polarity of three different solvents, n-hexane, ethyl acetate and methanol. Antioxidant activity of each extract was tested by using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method. Total phenol, total flavonoid and total tannin content of the extracts were also measured. Ethyl acetate extract was fractionated using vacuum liquid chromato-graphy for fractionation. Purification was performed using TLC preparative. Isolate then characterized using specific spray reagent, UV-Vis spectrophotometry and infrared spectrophotometry. Crude drug of Psidiumguajava contained flavonoid, tannin, quinone, saponin and steroid/ triterpenoid. Antioxidant activity of ethyl acetate extract is 65.63% with total phenol 4.25%, total flavonoid 0.53% and total tannin 1.16%. Antioxidant compound N was isolated from ethyl acetate extract.Antioxidant compound N was supposed to be aglycone flavone that has OH at C -4’, C-5 and C-7.Key words: Psidium guajava L. , antioxidant activity, ethyl acetate extract
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/660
10.14499/indonesianjpharm23iss1pp36-40
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 36-40
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/660/526
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/688
2020-01-27T02:53:31Z
3:ART
PREPARATION AND CHARACTERIZATION OF STAVUDINE ENTRAPPED LIPOSPHERES
Shegokar, Ranjita; C.U. Shah College of Pharmacy, S.N.D.T.
Women’s University, Santacruz (W), Mumbai-400049 India
Singh, Kamalinder; C.U. Shah College of Pharmacy, S.N.D.T.
Women’s University, Santacruz (W), Mumbai-400049 India
Lipospheres of stavudine were prepared by melt dispersion technique using trimyristin, tripalmitin and triastearin, stearic acid, Compritol® 888 ATO and Precirol® ATO 5 as lipid matrix in the various drug-lipid ratios. Drug entrapped free flowing solid lipospheres of triglycerides and glyceryl behenate were characterized for surface morphology, particle size distribution, encapsulation efficiency, and in vitrorelease behavior. The effect of drug lipid ratio, the surfactant used, concentration of stabilizer, and stirring speed were identified as the key variables affecting the formation of discrete spherical lipospheres and sustained drug release rate. The lipospheres production conditions were optimized by using 2% w/w sodium cholate and 1% Plural oleic as a stabilizer. The concentration of lipid used had pronounced effect on particle size of the lipospheres. The incorporation efficiency was found to be in range of 30 to 50%. Increase in concentration of surfactant and stirring speed produced fine spherical, smooth, and round lipospheres. All the prepared lipospheres exhibited slow release profiles dictating the Higuchi mode of release.Key words: Lipospheres, stavudine, lipid matrix, hydrophilic surfactant, drug release
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/688
10.14499/indonesianjpharm23iss4pp231-237
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 231-237
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/688/554
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1104
2018-10-23T02:18:57Z
3:ART
Population-Based Approach to Analyze Sparse Sampling Data in Biopharmaceutics and Pharmacokinetics using Monolix and NONMEM
Nugroho, Akhmad Kharis; Department of Pharmaceutics Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta Indonesia
Hakim, Arief Rahman; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada
Hakim, Lukman; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada
model, population, sparse sampling data, Monolix, NONMEM
Although it has been developed since 1972, the implementation of a population-based modeling approach in Indonesia, particularly to analyze biopharmaceutics and pharmacokinetics data is still very limited. This study was aimed to evaluate the performance of Monolix and NONMEM, two of the popular software packages in a population-based modeling approach, to analyze the limited data (sparse sampling data) of the time profiles of the simulated plasma drug concentration of a theoretical compound. and NONMEM were used to model the limited data (40 data points) as a results of the random selection from the 180 point data of simulated plasma drug concentration (Cp) on 20 subjects at 0.25; 0.5; 0.75; 1; 1.5; 3; 6; 12 and 18 hours after per-oral administration of a 100mg of a theoretical compound. Population values of the absorption rate constant (Ka), the elimination rate constant (Kel) and volume of distribution (Vd) were compared to the average Ka, Kel and Vd obtained by the conventional method (two stage approach) using PKSolver on the Cp data of all subjects. The calculation system of a nonlinear mixed effect model in Monolix and NONMEM, successfully describes the sparse data, based on the visual evaluation of the goodness of fit. Comparison of parameter estimates of population values in Monolix and NONMEM are in the range of 94 to 108% of the real values of the rich data analysed by PKSolver. A population-based modeling can adequately analyze limited or sparse data, demonstrating its capability as an important tool in clinical studies, involving patients.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-12-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1104
10.14499/indonesianjpharm28iss4pp205
Indonesian Journal of Pharmacy; Vol 28 No 4, 2017; 205
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1104/853
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1349
2019-10-10T01:29:33Z
3:ART
The Properties of Brown Marine Algae Sargassum turbinarioides and Sargassum ilicifolium Collected From Yogyakarta, Indonesia
Artemisia, Rahma; Stikes Madani Yogyakarta
Jl. Wonosari KM 10, Karanggayam, Sitimulyo, Piyungan, Bantul, DIY
Nugroho, Akhmad Kharis; Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
Setyowati, Erna Prawita; Department of Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
Martien, Ronny; Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
Sargassum turbinarioides, Sargassum ilicifolium, Fucoidan, Thin Layer Chromatography (TLC), Fourier transform infrared (FTIR) spectroscopy
Brown marine algae are the prominent source of marine natural products that have bioactive metabolites. Sargassum turbinarioides and Sargassum ilicifolium were dominated in Indonesia as brown marine algae that well known as a source of fucoidan. The samples were collected from Gunungkidul, Yogyakarta, Indonesia. In this study, we investigated and identified the yield of aqueous crude and purified extracts using different extraction temperatures (60°C, 70°C, 80°C, 90 °C). The highest yield of S. turbinarioides crude extract (7.36%) was obtained at temperatures 90 and 80 °C while the highest yield of S. ilicifolium was 3.49 % at 80 °C. The presence of sulfate polysaccharide in Sargassum turbinarioides is 3,78 % and Sargassum ilicifolium is 2,93 %. Each of the extract was screened using phytochemical detection, Thin Layer Chromatography (TLC) and Fourier transform infrared spectroscopy (FT-IR) analysis. The phytochemical detection indicated that Sargassum ilicifolium has bioactive metabolites such as carbohydrates, proteins and amino acids, terpenoid, phenolic compounds, and flavonoids. The FTIR spectrum of the S. turbinarioides and Sargassum ilicifolium extract refer to the presence of ester sulfate groups through showing peaks at 1300 to 1200 cm-1 and 980 to 950 cm-1. The result indicated that Sargassum turbinarioides and Sargassum ilicifolium contain of sulfate polysaccharide were prospect a biological activities to use for the development of marine nutraceutical drugs especially as antioxidant.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Ministry of Research, Technology, and Higher Education, Republic of Indonesia and also Beasiswa Unggulan Dosen Indonesia - Dalam Negeri (BUDI-DN) from Lembaga Pengelola Dana Pendidikan (LPDP) for sponsorship and financial support
2019-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1349
10.14499/indonesianjpharm30iss1pp43
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 43
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1349/892
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1988
2021-01-07T01:00:39Z
3:ART
In vitro Comparative Study for Anti-proliferative Activity of Some Plant Extracts, Fam. Apiaceae, on HeLa Cell Line
Gomaa, Sara E.; Medicinal and Aromatic Plants Research Dept., Horticulture Research Institute (HRI), Egypt. 9 ALgamaa Street, Giza, Egypt
Friedersdorf, Matthew; Molecular Genetics and Microbiology Dept., Duke University Medical Center, NC, USA
El Enshasy, Hesham Ali; Director,
Institute of Bioproduct Development (IBD), Universit Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Abou-Donia, MB; Pharmacology and Cancer Biology Dept., Duke University School of Medicine, NC, USA.
Apiaceae - Anti-proliferative - Extracts - HeLa Cell Line
In this research, the biological activities of five plant extracts from family Apiaceae; Italian Parsley (Petroselinum neapolitanum), Fennel (Foeniculum vulgare), Celery (Apium graveolens), Cilantro (Coriandrum sativum) and Dill (Anethum graveolens), were studied. Antiproliferative effect of eleven ethanol crude extracts was tested in Human Cervical (Hela) cancer cells. Results clearly demonstrated that all plant extracts showed high significant difference when compared to the negative control (DMSO).Parsley leaves extract, cilantro leaves extract and cilantro stems extract showed no significant difference with the positive control (Actinomycin D). As for, fennel bulb extracts, fennel stalks extracts, celery stems gave better results than the positive control with no significant difference through the 24, 48 and 72 h treatment. There were no significant difference between Fennel extracts and the positive control, which showed high effect on the cancer cells survival. There were no significant difference between both extracts of Cilantro leaves and stems through each time but the best result was after 72 h of treatments. Regarding Dill leaves and stems, cell numbers recorded no significant difference between the both on time dependent manner. Further investigation for ethanolic extracts of parsley leaves, fennel bulb, fennel stalks, celery stems, cilantro leaves and cilantro stems which showed better results than using the commercial drug Actinomycin D (25ml/ml) for 24 h treatment or less depending on concentrations manner. Also, further investigation on different types of cancer cell lines to avoid the toxic effect of chemotherapy.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Jack Kenee, Duke University, USA
2020-08-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1988
10.14499/indonesianjpharm31iss2pp108
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 108
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1988/946
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/666
2020-01-27T02:51:49Z
3:ART
BIOLOGICAL IMPORTANCE AND HEALTH EFFECT OF Perilla frutescence PLANT
Asif, Mohammad; Department of pharmacy, GRD (PG) IMT, Dehradun, Uttarakhand, India
The study was designed to study the Biological importance and nutritional benefits of Perilla frutescence plant. This plant has very high biological activities. It contains various phytoconstituents such as different phenolic compounds (like rosemarinic acids, luteolin chrysoeriol, quercetin, catcehin, apigenin, etc), vitamins, minerals etc. Perilla seeds contained 30-40% fixed oil, contained both saturated (palmitic acid 5-7%, stearic acid 1-3%), unsaturated (UFAs) or polyunsaturated (PUFAs) (oleic acid 12-22%, linoleic acid 13-20%, γ-linolenic acid 0-1%, α-linolenic acid 52-64%, icosanoic acid 0-1%) fatty acids. Perilla oil is highly unsaturated oil among the all current discovered natural vegetable oils. Whole perilla plant has many important functions on improving and accelerating human health and is effective in prevention of various diseases.Key words: Perilla frutescence, biological activities, phyto-constituents, polyphenols, polyunsatured fatty acids.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/666
10.14499/indonesianjpharm23iss2pp84-92
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 84-92
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/666/532
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1310
2018-11-02T06:46:53Z
3:ART
Derivatives of 3-(alkylthio)-5-(thiophen-2-ylmethyl)-4H-1,2,4-triazol-4-amines as increasing efficiency substances
Safonov, Andrey; Zaporizhzhia State Medical Univercity
1,2,4-triazole, pharmacological correction of fatigue, method of forced swimming
In today's society, especially in eastern countries such as China, Japan etc., the problem of fatigue and even death at work is acute. One of the solution to this problem is complex therapy with increasing efficiency drugs. Derivatives of 1,2,4-triazole have already proven themselves as potential compounds for pharmacological correction of fatigue. Compounds were synthesized at the Department of toxicological and inorganic chemistry ZSMU. Using method of forced swimming on a group of white nonlinear rats activity of anti-fatigue of the compounds were analyzed. Having analyzed the data of pharmacological correction of fatigue for 3-(alkylthio)-5-(thiophen-2-ylmethyl)-4H-1,2,4-triazol-4-amines, it should be noted that this series of compound do not show anti-fatigue effect. Introduction in molecule 3- (nonylthio)-5-(thiophen-2-ylmethyl)-4H- 1, 2, 4-triazol-4-amine 4-fluorbenzylidene, 4-(dimethylamino) benzylidene, 4- methoxybenzylidene, 2-chloro-6-fluorobenzylidene radicals lead to increase actoprotective effect. The most active compound is N-(2-chloro-6-fluorobenzylidene)-3-(nonylthio)-5-(thiophen- 2- ylmethyl)- 4H- 1, 2, 4- triazol- 4-amine which exceeds the standard (riboxin).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Zaporizhzhia State Medical University
2018-09-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1310
10.14499/indonesianjpharm29iss3pp167
Indonesian Journal of Pharmacy; Vol 29 No 3, 2018; 167
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1310/878
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1467
2019-10-28T01:20:02Z
3:ART
Anticancer Molecules from Catharanthus roseus
Taher, Zarani M; Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Agouillal, Farid; 1. Research Unit on Analysis and Technological Development in Environment, URADTE, Centre de Recherche Scientifique et Technique en Analyses Physico-Chimiques, CRAPC, B.P. 384, RP 42004 Zone Industrielle Bou-Ismail, Tipaza, Algeria.
2. Laboratory of Reaction Engineering, LGR, Faculty of Mechanical Engineering and Process Engineering, Houari Boumediene University of Science and Technology, USTHB, PB 32 El Alia, 16075, Bab Ezzouar, Algiers, Algeria.
R, Lim J.; School of Chemical Engineering and Energy, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Marof, Aina Q; Department of Chemistry, Mathematics and Science Faculty, Universitas Negeri Jakarta, Jakarta, Indonesia
Dailin, Daniel Joe; 1. Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
2. School of Chemical Engineering and Energy, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Nurjayadi, Muktiningsih; Department of Chemistry, Mathematics and Science Faculty, Universitas Negeri Jakarta, Jakarta, Indonesia
Razif, Ezzaty NM; Faculty of Applied Sciences, Universiti Teknologi Mara Negeri Sembilan, Kampus Kuala Pilah, Pekan Parit Tinggi 72000, Kuala Pilah Negeri Sembilan, Malaysia
Gomaa, Sara E; Horticulture Research Institute, Agriculture Research Center, Egypt.
El Enshasy, Hesham Ali; Director
Institute of Bioproduct Development (IBD)
Universiti Teknologi Malaysia (UTM)
Skudai, Johor Bahru, Malaysia
C. roseus; Anticancer; Extraction; Phytochemical; Tissue culture
Catharanthus roseus is an important medicinal plant found in various parts of the world and the bioactive compound has been extracted and used as anti-cancer agent to treat the cancer over decades. However, the extraction of bioactive compound also results in the generation of large quantities of pollution with wasted solvents. Toxic pollution occurs when synthetic chemicals are discharged or natural chemicals accumulate to toxic levels in the environment, causing reductions in wildlife numbers, degrading ecosystem functions and threatening human health. This review covers the extraction and phytochemical obtained leading to chemical compounds related to anti-cancer property of C. roseus. Additionally, recent advances of using biological cell cultures were also addressed. Thus, this work can be used for further investigation of C. roseus to be undertaken in future for its anti-cancer property further development and efficient production in drug industry
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
MOE and UTM-RMC (Malaysia) through HICOE grant no. R.J130000.7846.4J262.
2019-07-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1467
10.14499/indonesianjpharm30iss3pp147
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 147
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1467/910
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/683
2020-01-27T02:53:31Z
3:ART
REVIEW OF BIOLOGICAL ACTIVITIES OF HYDRAZONES
Ali, Md. Rahmat; Department of Pharmaceutical Chemistry,
Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
Marella, Akranth; Department of Pharmaceutical Chemistry,
Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
Alam, Md. Tauquir; Department of Pharmaceutical Chemistry,
Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
Naz, Ruksar; Department of Pharmaceutical Chemistry,
Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
Akhter, Mymoona; Department of Pharmaceutical Chemistry,
Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
Shaquiquzzaman, Md.; Department of Pharmaceutical Chemistry,
Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
Saha, Rikta; Department of Pharmaceutical Chemistry,
Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
Tanwar, Omprakash; Department of Pharmaceutical Chemistry,
Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
Alam, Md. Mumtaz; Department of Pharmaceutical Chemistry,
Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
Hooda, Jyoti; CHRD- Competitive and Scientific Intelligence
(CSI), WNS Global Services (P) Ltd, Gurgaon
122002, India.
Hydrazones possess an azomethine –NHN=CH group and are considered as derivatives of aldehydes and ketones in which the oxygen atom has been replaced by the NNH2 functional group. These are widely studied molecules owing to their ease of preparation and diverse pharmacological potential. This has led researchers to synthesize different heterocyclic compounds bearing hydrazones. Medicinal chemists across the world have done immense work on hydrazones and developed agents with better activity and low toxicity profiles. Following different synthetic protocols and through proper SAR studies differently substituted hydrazones have been developed and found to be active against different pharmcological targets. They are known to possess different biological activities viz. antimicrobial, antiinflammatory, anticancer, antimalarial etc. These observations have been guiding for the development of new hydrazones that possess varied biological activities. The review aims at highlighting the diverse biological activities of hydrazones.Key words: Azomethine, Hydrazones, Pharmacological Potential
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/683
10.14499/indonesianjpharm23iss4pp193-202
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 193-202
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/683/549
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1470
2022-10-26T01:05:53Z
3:ART
Quality of Life of Patient with Hypertension in Primary Health Care in Bandar Lampung
Khoirunnisa, Sudewi Mukaromah; Institut Teknologi Sumatera
Akhmad, Atika Dalili; Institut Teknologi Sumatera
Hypertension; Quality of Life; SF-36
The high prevalence of hypertension in Indonesia, encourages studies related to how the quality of life of patients with hypertension. The purpose of this study was to measure quality of life, identify and explain factors related to the quality of life of patients with hypertension.The study design was descriptive correlation using a cross sectional study approach. The study subjects were all outpatient hypertensive patients several health centers in Bandar Lampung. The instrument used in this study was the Indonesian SF-36. The data collected included the patient's demographic characteristics including gender, age, education, occupation, and marital status, and the fields related to the health history of the study subjects included the duration of hypertension, complications, and the number of antihypertensive drugs consumed. Data were analyzed using bivariate analysis to see the relationship between two variables with the level of significance used was 5% (α = 0.05) with the value of the confidence interval set was 95%. Multivariate analysis was conducted to study the relationship of several independent variables with one or several dependent variables.The results of univariate analysis showed that age, marital status, duration of illness, complications, and the number of drugs consumed had an effect on the quality of life of hypertensive patients (p <0.05). The results of multivariate analysis showed that the factors of age, marital status, and duration of hypertension are factors that influence the physical domain, while the factors of gender, marital status, duration of hypertension, complications, and the number of drugs are influential factors in the mental domain mental.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
The Ministries of Research, Technology and Higher Education of Indonesia
2020-01-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1470
10.14499/indonesianjpharm30iss4pp309
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 309
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1470/927
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1470/200
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/506
2018-09-05T01:49:54Z
3:ART
SUPER POROUS HYDROGELS: A RECENT ADVANCEMENT IN GASTRORETENTIVE DRUG DELIVERY SYSTEM
Mayur, Chordiya; Dept of Pharmaceutics, C.L. Baid Metha College of Pharmacy, Thorapakkam, Chennai, 600096., India. Dept of Pharmaceutics,K.K.
K, Senthilkumaran; Dept of Pharmaceutics,K.K. College of Pharmacy, Mangadu, Chennai, 600096, India.
Hemant, Gangurde; Dept of Pharmaceutics, Nandha Collegeof Pharmacy, Erode, 638052 India.
Super porous hydrogels (SPHs) were originally developed as a controlled drug delivery system to retain drugs in gastic medium. Super porous hydrogels (SPHs) are recent advancement in gastro retentive drug delivery system (GRDDS) which also includes intragastric floating system (low density system), mucoadhesive system, high density system and swellable system. Super porous hydrogels should instantly swell in the stomach and maintain their integrity in the harsh environment and release the pharmaceutical active ingredient. SPH swell fast, within minutes, the fast swelling property is based on water absorption through open porous structure by capillary force. This review discusses about the GRDDS, difference between gels and hydrogels and comparison between SAP vs. SPH. It also includes types of SPH, different generations, general synthesis, methods of preparations, gastric emptying, advantages, characterization, applications and salient features of SPH. Key words: Superabsorbent polymers (SAP), Super porous hydrogel (SPH), Gastro retentive drug delivery system
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2013-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/506
10.14499/indonesianjpharm24iss1pp1-13
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 1-13
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/506/384
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1367
2019-10-03T03:16:50Z
3:ART
α-Glucosidase Inhibition Activity of Stem Bark Ceiba pentandra Linn. In Vitro and In Silico
Syihabudin, Vicri; Pakuan University
α-glucosidase; AutoDock Vina; LCMS/MS; vavain derivative
Ceiba pentandra (L.) stem bark plant contains tannin, flavonoid, alkaloid, terpenoid, and saponin compounds. It is known that flavonoid compounds have a potential for inhibition activity of an α-glucosidase enzyme. The purpose of this research is to know the α-glucosidase inhibition activity of the fraction of n-hexane, ethyl acetate, and water. These three fractions measured α-glucosidase inhibition activity by spectrophotometric UV-vis method. The fraction extract with IC50 values can best be done an analysis of active compounds using LCMS/MS methods, and results of the suspected compound do the binding molecule with a receptor model isomaltase α-glucosidase uses AutoDock Vina so that bond can predict drug active compounds. The results of this research activity are inhibition of α-glucosidase stem bark in the fraction of n-hexane 4,60 μg/mL, ethyl acetate fraction 8,55 μg/mL and fractions of the water 5,61 μg/mL. Analysis of the compound fraction of water by LCMS/MS eluted at the retention time 3,61 seconds allegedly derivative compounds (+) catechin and on retention time 8,70 minutes allegedly derivative compounds vavain. Vavain derivative compounds, (+) catechin, acarbose and quercetin each has its own docking score of -8,1; -8,8; -6,2 and -7,6 kcal/mol and has a semblance of bonding on amino acid Glu276 which is a residue catalytic role in hydrolysis reactions. Equality on the sides of active chemical bonds is expected to have the same activity as drug inhibition of the enzyme α-glucosidase.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Bina Lohita Sari, Novi Fajar Utami, Purnama Alam and Utari
2018-12-17
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1367
10.14499/indonesianjpharm29iss4pp206
Indonesian Journal of Pharmacy; Vol 29 No 4, 2018; 206
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1367/885
Copyright (c) 2018 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1903
2021-01-06T13:13:53Z
3:ART
In Vitro Antiplasmodial Activity and Cytotoxicity of Active Subfractions of Harmsiopanax aculeatus Leaves
Turalely, Rachel; Biotechnology Study Program, Graduate School, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Chemistry Education Study Program, Faculty of Teacher Training and Education Science, Pattimura University, Ambon, Maluku, Indonesia
Wijayanti, Mahardika Agus; Department of Parasitology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada,
Hertiani, Triana; Faculty of Pharmacy, Universitas Gadjah Mada
Mustofa, Musthofa; Department of Pharmacology and Therapy, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada Yogyakarta, Indonesia.
H. aculeatus, antiplasmodial activity, cytotoxicity, malaria, subfractions
Harmsiopanax aculeatus leaves, a medicinal plant with locally named kapur, have been used traditionally to treat malaria in Maluku, Indonesia. However, the scientific information of this plant is still limited. In our previous study, the methanol extract of this plant leaves have been proven to possess in vitro antiplasmodial activity. This study was conducted to evaluate in vitro antiplasmodial activity and cytotoxicity of subfractions of the plant leaves. Fractionation was performed using a column chromatography with Sephadex LH-20 as the stationary phase and methanol as the mobile phase. The subfractions obtained were then tested for in vitro antiplasmodial activity on a chloroquine-resistant FCR3 strain of Plasmodium falciparum using a visual method. Cytotoxicity was evaluated by using MTT assay. The in vitro antiplasmodial activity and cytotoxicity were expressed as IC50, calculated using probit analysis with SPSS 16 for windows. The results showed that the four subfractions tested have a high antiplasmodial activity with IC50 values of 0.09; 0.18; 0.01; and 0.77 µg.mL-1, respectively. In addition, these subfractions had IC50 values of >400 µg.mL-1 against Vero cells indicating that they were non-toxic. In conclusion, the subfractions of H. aculeatus leaves are very active and selective against P. falciparum. Further study will be conducted to isolate the active compounds.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-04-09
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1903
10.14499/indonesianjpharm31iss1pp51
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 51
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1903/944
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/521
2018-09-05T01:49:55Z
3:ART
EVALUATION OF ANTIMICROBIAL POTENTIALS OF Cardiospermum halicacabum Linn.
Veerappan, Loganathan; School of Pharmacy, Taylor’s University, 47500 Subang Jaya, Selangor, Malaysia.
T, Thirunala Sundari; Dept. of Biotechnology, Bharathidasan University, Tiruchirapalli, Tamil Nadu, 620 024, India.
N, Pradheepa; Dept. of Biotechnology, Mother Theresa Women University, Kodaikanal, 624101 India.
Antimicrobial activity of Cardiospermum halicacabum shoot extracts were studied on gram-positive bacteria such as Staphylococcus aureus, Bacillus substilis, and gram-negative bacteria such as E.coli, Proteus vulgaris, and fungus Candida albicans. Disc diffusion method was used to study the antimicrobial activity of aqueous, ethanol, chloroform and ether extracts of C. halicacabum. Ampicillin was used as reference standard at 10 mg/disc concentration. Extracts of C. halicacabum exhibited a significant antibacterial activity except the aqueous extract. Ethanolic extract was found to be very effective with maximum activity index (0.84). The ethanolic extract exhibited minimum inhibitory concentration (MIC of 0.25 mg/mL against Staphylococcus aureus, Bacillus substilis, E. coli and Proteus vulgaris and 0.125 mg/mL against Candida albicans. The MIC of chloroform and ether extracts ranged between 0.25 and 1.0 mg/ml against the test organisms. All the extracts showed antifungal activity against Candida albicans. Key words: antimicrobial activity, Cardiospermum halicacabum shoot system, solvent extraction,.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2013-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/521
10.14499/indonesianjpharm24iss1pp61-64
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 61-64
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/521/399
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1329
2018-10-23T02:19:33Z
3:ART
Effects of Probiotics and Vitamin B Supplementation on IFN-γ and IL-12 Levels During Intensive Phase Treatment of Tuberculosis
Suprapti, Budi; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Suharjono, Suharjono; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Raising, Rahmawati; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Yulistiani, Yulistiani; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Izzah, Zamrotul; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Nilamsari, Wenny Putri; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Wulaningrum, Prastuti Asta; Department of Pulmonology and Respiratory Medicine, Universitas Airlangga Teaching Hospital, Surabaya
Bachtiar, Arief; Department of Pulmonology and Respiratory Medicine, Universitas Airlangga Teaching Hospital, Surabaya
Interleukin-12, Interferon-γ, Probiotics, Tuberculosis, Vitamin B
Tuberculosis is an acute infectious disease that primarily affects the lungs. Probiotics supplementation can increase the number and activity of NK cell in peripheral blood by modulation of IL-12, thus increasing IFN-γ production by Th1 response. Vitamin B1 acts on macrophages and affects neutrophil motility. Vitamin B6 is associated with the release of cytokines and the responsiveness of NK cells, while vitamin B12 affects to lymphocytes, Tcell proliferation, CD4+ ratios, and NK cell activity. To analyze the effects of probiotics and vitamin B1, B6, B12 supplementation on IFN-γ and IL-12 levels during intensive phase of antituberculosis treatment. The study was pre-post test randomised control by time series. The control group was TB patients with standard therapy of antituberculosis and vitamin B6, while the intervention group was TB patients receiving therapy plus once daily probiotics and vitamin B1, B6, B12supplementation during the intensive phase. Blood samples were withdrawn at baseline, one month, and two months after therapy to measure plasma IFN-γ and IL-12 levels using the ELISA method. Twenty two patients were divided equally into two groups. There was a tendency to greater increase of IFN-γ in the first month of the intervention group, followed by a significant decline after two-month therapy (p < 0.05). In both groups there was a rise in IL-12 levels after one month followed by a decrease in the second month (p > 0.05). However, the percentage was higher in the supplementation group. Adding probiotics and vitamins B1, B6, B12 could improve immune response through IL-12 and IFN-γ modulation during intensive phase therapy.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-06-22
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1329
10.14499/indonesianjpharm29iss2pp80
Indonesian Journal of Pharmacy; Vol 29 No 2, 2018; 80
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1329/868
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1462
2019-10-10T01:30:23Z
3:ART
Effect of Platelet-rich Plasma on Caspase-3 and IGF-1 mRNA expression in the diabetic rat testis
Istiqamah, Evi; Postgraduate Student of Master Biomedical Science, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Department of Midwifery, Faculty of Public Health, Universitas Muslim Indonesia Makassar, Indonesia
Rizal, Dicky Moch.; Department of Physiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Puspitasari, Ika; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
PRP, diabetic rat testis, caspase-3 mRNA expression, IGF-1, mRNA in expression
Testicular damage is a serious complication of diabetes mellitus resulting in male infertility, which is associated with caspase-3 and IGF-1 mRNA expression. Platelet-rich plasma (PRP), with its rich growth factor composition, has proven beneficial in regenerative therapy. It is believed that PRP has not been studied in testes for diabetes mellitus and there are no studies in the literature concerning the influence of PRP on expressions of growth factors in testes.The aim of this study was to investigate the efficacy of adjunctive PRP in insulin treatment for repair of testicular damage in a diabetic rat model. Diabetes was induced by administering single dose 60 mg/kg streptozotocin. Twenty Wistar male rats were divided into four groups: group 1, control group; group 2, diabetes without treatment; group 3, diabetes with treated insulin; and group 4, diabetes with treated insulin and PRP. Rats were euthanized after two weeks of treatment, and testes were taken for caspase-3 and IGF-1 mRNA expression measurements.Diabetes mellitus induction caused a significant increase in caspase-3 mRNA expression with p=0.049 and significant decrease in IGF-1 mRNA expression with p=0.004. There was no difference in caspase-3 and IGF-1 mRNA expression of the diabetic rat testis given insulin and PRP compared to without PRP.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-05-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1462
10.14499/indonesianjpharm30iss2pp98-104
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 98-104
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1462/902
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/678
2020-01-27T02:53:16Z
3:ART
Satyrium nepalense: A RARE MEDICINAL ORCHID OF WESTERN HIMALAYA (INDIA); PHYTOCHEMICAL SCREENING, ANTIMICROBIAL EVALUATION AND CONSERVATION STUDIES
Mishra, Abhay Prakash; Department of Pharmaceutical Chemistry, H.N.B. Garhwal (A Central) University Srinagar, Uttarakhand, INDIA. 246174
Saklani, Sarla; Department of Pharmaceutical Chemistry, H.N.B. Garhwal (A Central) University Srinagar, Uttarakhand, INDIA. 246174
Satyrium nepalense is an endangered medicinal herb found at the higher altitude of 2400-5000m. Local inhabitant used tubers of Satyrium nepalense as an energetic tonic and as an important medicine to cure different type of fever in traditional health care system of Uttarakhand (INDIA). Present study was carried out to evaluate the phytochemical, antibacterial screening against four (Streptococcus mutans, Pseudomonas aeruginosa, Staphylococcus aureus and Klebsiella pneumoniae) microorganisms in methanolic extracts and habitat studies for conservation of S. nepalense. Tubers of Satyrium nepalense were extracted separately with methanol by hot extraction process using soxhlet apparatus. The extracts were subjected to Lyophilization to get dry extract and preserved in aseptic condition. The different group reagents used for phytochemical screening as dragendorffs’s for alkaloid, molisch’s test for carbohydrates, shinoda test for flavonoids etc. Antibacterial study was carried out by disc diffusion method. The highest zone of inhibition was recorded as 15.0±0.00 mm against Klebsiella pneumonia and 15.0±0.82 mm against Staphylococcus aureus. Phytochemical screening shows the presence of alkaloids, carbohydrates/glycosides, flavonoids and unsaturated sterols/triterpenes in Satyrium nepalense. The result indicates that methanolic extract of Satyrium nepalense shows potent antibacterial activity against all four bacterial strains.Key words: Uttarakhand, Satyrium nepalense, medicinal orchid, antibacterial, phytochemicals
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/678
10.14499/indonesianjpharm23iss3pp162-170
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 162-170
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/678/544
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1564
2022-10-26T01:05:53Z
3:ART
Response Surface Methodology used in the Optimization of RP-HPLC Condition for Quantitative Analysis of Carmine and Rhodamine B
Nuvitasari, Reyna
Rohman, Abdul; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Martono, Sudibyo
The objective of this study was to optimize reversed-phase high-performance liquid chromatography (RP-HPLC) using an experimental design approach based on the response surface methodology of Central Composite Design (CCD) for separation and analysis of carmine (CAR) and Rhodamine B (RHO) in lipstick products. Some factors (independent variables) responsible for RP-HPLC separation including pH of buffer phosphate (X1), the acetonitrile ratio (X2), flow rate of mobile phase (X3), and column temperature (X4) were investigated. While, the responses (dependent variables) evaluated were resolution between CAR and RHO (Y1), tailing factor of CAR (Y2), tailing factor of RHO (Y3), retention time of CAR (Y4), retention time of RHO (Y5), peak area of CAR (Y6) and peak area of RHO (Y7). CCD showed that separation of CAR and RHO was influenced by these independent variables (factors). The optimum predicted conditions for the separation of CAR and RHO based on statistical results was pH buffer of 3.4, ACN 55%, the flow rate of 1.1 mL/min and column temperature of 35oC with the desirability of 1. Both CAR and RHO were clearly separated using optimum conditions, as suggested by CCD. The developed techniques were effective for optimizing chromatographic separation, therefore, the time consumption and a large number of running could be hindered.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-10-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1564
10.14499/indonesianjpharm30iss4pp276
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 276
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1564/922
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/430
2020-02-19T02:37:07Z
3:ART
NEW 6-(3-INDOLYL)BENZO[B]CARBAZOLES
Santoso, Mardi; Chemistry Department, Faculty of Science,
Sepuluh Nopember Institute of Technology Surabaya
Some attempts have been carried out to synthesize polycyclic heterocyclic compounds derived from new benzocarbazoles (1). New 6-(3-indolyl)benzo[b]-carbazoles (2a) have been synthesised in good yields from the reaction of simple indoles and o-phthaldialdehid in methanol catalysed by p-toulenesulfonic acid monohydrate. Their precise structure were established by microanalysis, spectroscopy, and X-ray crystallography of the benzo[b]carbazole (2a).Keywords: indoles, polycyclic heterocyclic compounds, benzo[b]carbazoles
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/430
10.14499/indonesianjpharm0iss0pp1-9
Indonesian Journal of Pharmacy; Vol 12 No 1, 2001; 1-9
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/430/309
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1273
2018-10-23T02:18:57Z
3:ART
Review of Insulin Therapy In Type 2 Diabetes Mellitus Ambulatory Patients
Suprapti, Budi; Faculty of Pharmacy Universitas Airlangga, Jalan Dharmawangsa Dalam, Surabaya, Indonesia
Widyasari, Nia; Faculty of Pharmacy Universitas Airlangga, Jalan Dharmawangsa Dalam, Surabaya, Indonesia
Rahmadi, Mahardian; Faculty of Pharmacy Universitas Airlangga, Jalan Dharmawangsa Dalam, Surabaya, Indonesia
Wibisono, Cahyo; Faculty of Medicine Universitas Airlangga, Jalan Dr. Moestopo, Surabaya, Indoensia
Diabetes mellitus, insulin, ambulatory patient, DUR, DRP
The purposes of this study were to review utilization of insulin therapy in type 2 diabetes mellitus out patients and identify its drug related problems. The data were collected cross-sectionally with purposive sampling method in the period March 2016 until May 2016 in Outpatient Clinic Universitas Airlangga Teaching Hospital Surabaya. The results of 240 patients showed that insulin was used as monotherapy insulin in 2,9% patients; combination 1 insulin & 1-4 OAD in 31,3%; basal bolus therapy 27,9%; combination basal−bolus therapy & 1-3 OAD 43,9%. Based on blood glucose target achievement, only 20,8% of patients achieve the target, 75,1% failed to achieve the target and 4,1% suffered from hypoglycemia. Drug related problems identified adverse drug reaction of antidiabetic therapy such as hypoglycemia (6.7%), nausea (3.8%), bloating (1.3%), increase of flatulency (2.9%) and inappropriate combination (0,4%). In conclusion insulin therapy was complicated and individually, most of the patients still did not reach the target and there was potential drug related problem in this patients group. So that caring from solid inter-professional health collaboration is needed
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Faculty of Pharmacy Universitas Airlangga, Jalan Dharmawangsa Dalam, Surabaya, Indonesia
2017-12-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1273
10.14499/indonesianjpharm28iss4pp221
Indonesian Journal of Pharmacy; Vol 28 No 4, 2017; 221
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1273/855
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1601
2021-01-07T01:00:39Z
3:ART
FTIR Method for Peptide Content Estimation and Degradation Kinetic Study of Canarium Nut Protein
Oktaviary, Rozana
Nugrahani, Ilma
Surantaatmadja, Slamet Ibrahim
Kartawinata, Tutus Gusdinar
Apsari, Cintya Nurul
Canarium nut; peptide; degradation; FTIR; kinetic
Quantitative analysis of bioactive peptide mostly conducted by measuring the activity. While the determination of peptide content in natural sources has been conducted using various instruments, vibrational spectroscopy remains underutilized. Here, we attempted new developed method in peptide quantification and degradation kinetic analysis using Fourier-Transform Infrared Spectroscopy. Bovine Serum Albumin was used as standard protein in method development and validation. Peptide content was estimated by converting peak area to concentration. The method was used to estimate peptide content in Canarium nut protein and its hydrolysates, which potentially hold biological activity. Kinetic study was conducted with microwave as an accelerator for hydrolysis, an apparatus rarely used in peptide study. Amide I band on wavenumber range of 1724.05-1619.91 cm-1 was selected for analysis, considering its selectivity and linearity. The method also met other validation requirement, including accuracy and precision. When applied in quantitative analysis, the method was able to calculate peptide content decrease in Canarium nut protein after hydrolysis using papain (38.24%), pepsin (33.67%) and alkaline reagent (28.53%). In kinetic study, microwave-assisted peptide degradation exhibited logarithmic profile with the equation of y=-0.148ln(x)+0.9591 and R² value of 0.963. Based on these results, FTIR is useful in estimating peptide content and in analyzing degradation kinetic profile.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-07-10
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1601
10.14499/indonesianjpharm31iss2pp78
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 78
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1601/949
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1355
2018-11-02T06:46:53Z
3:ART
The Effect of Ethanol Extract of Piper nigrum L. Fruit on Reproductive System in Adult Male Wistar Rats: A Study of FSH, LH, Testosterone Level and Spermatogenic Cells
Ekaputri, Tia Wida; Master Programme, Basic Medical and Biomedical Science, Faculty of Medicine, Universitas Gadjah Mada, Indonesia
Sari, Ika Puspita; Department of Pharmacology & Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada
Rizal, Dicky Moch.; Department of Physiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Indonesia
black pepper (Piper nigrum L.), FSH, LH, male rat, testosterone, spermatogenic cells
Exploration to find out new natural contraceptive agent for male is still being developed. Black pepper (Piper nigrum L.) and its main alkaloid piperine have potential antifertility because of sitotoxic and hormonal effects. The aim of this study is to find out the effect of ethanolic extract of black pepper (Piper nigrum L.) fruit of on reproductive hormone serum level, sperm quality, and spermatogenic cell populations in adult male Wistar rat. Twenty five male rats were divided into five groups consisting of two control group, i.e. K(-) (Na-CMC 0.5%), K(+) (finasteride 0.45mg/kg BW), and three groups received different doses of black pepper fruit ethanolic extract, i.e. D(1) (3.33mg/kg BW), D(2) (6.66mg/kg BW) and D(3) (13.32mg/kg BW) respectively. The treatment were given to each group for 55 days. Reproductive parameters were measured, including serum level of reproductive hormones (FSH, LH, dan testosteron), quality of cauda epididymal sperm (spermatozoa concentration, motility and morphological abnormality), spermatogenic cell populations (primary spermato-cyte and spermatid count) and seminiferous tubules diameter. Ethanol extract of black pepper fruit at doses of 3.33mg/kg BW, 6.66mg/kg BW, and 13.32mg/kg BW increased serum FSH level. Extract at dose of 13.32mg/kg BW decreased serum LH level, while extract at doses of 6.66mg/kg BW and 13.32mg/kg BW decreased serum testosterone level. The number of primary spermatocytes, spermatozoa concentration, and spermatozoa motility were decreased by administration of ethanol extract of black pepper fruit with dose of 6.66mg/kg BW and 13.32mg/kg BW. Ethanol extract of black pepper fruit at dose of 6.66mg/kg BW and 13.32mg/kg BW had a negative impact on the male reproductive system and showing potential antifertility in male rat.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-09-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1355
10.14499/indonesianjpharm29iss3pp136
Indonesian Journal of Pharmacy; Vol 29 No 3, 2018; 136
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1355/875
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1289
2018-10-23T02:19:20Z
3:ART
In Vitro Evaluation of Anti-Inflammatory and Anti-Diabetic Potential Effects of Euphorbia Tithymaloides Ethanol Extract
Wandita, Theresia Galuh; Departement of Animal Life and Environmental Science, HANKYONG NATIONAL UNIVERSITY, SOUTH KOREA
Joshi, Najuma; Departement of Animal Life and Environmental Science, HANKYONG NATIONAL UNIVERSITY, SOUTH KOREA
dela Cruz, Joseph Flores; Department of Basic Veterinary Sciences
College of Veterinary Medicine
University of the Philippines Los Banos, PHILIPPINES
Hwang, Seong Gu; Departement of Animal Life and Environmental Science, HANKYONG NATIONAL UNIVERSITY, SOUTH KOREA
Hibiscus rosasinensis, glipizide, mucilage, antidiabetic activity, excipients
This research work aims to develop glipizide tablets using Hibiscus rosasinensis leaves mucilage. The mucilage was extracted by using double distilled water and precipitated with ethanol. The precipitated mucilage was dried and grounded into powder. The tablet utilizing the mucilage as excipient was prepared by wet granulation method. The tablets were subjected to various tests. The evaluatory parameters of tablets were found to be within the limits as per United States Pharmacopoeia NF 24/19. FTIR spectrum reveals that there is no incompatibility between the ingredients used. Diabetes was induced in wistar albino rats using streptozotocin and effect of formulation on blood glucose level was determined. It was observed that the formulation could not sustain the release of glipizide. However, the glipizide release was retarded as the amount of mucilage was increased. It was observed that on the completion of antidiabetic study, the formulation could bring the blood glucose level to normal in group rats. However, the blood glucose level was still elevated in group administered with pure gliplizide. It can be concluded that HRM can be used to formulate glipizide tablets. The formulations with HRM shows better hypoglycemic activity and this may be attributed to antidiabetic activity of Hibiscus rosasinensis.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-03-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1289
10.14499/indonesianjpharm29iss1pp1
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 1
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1289/858
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1521
2019-10-10T01:30:23Z
3:ART
The Effect of Thionamide to TRH, TSH, IL-4, T-REG, and Anti-TPO in Graves’ Disease
Decroli, Eva; Endocrinology and Metabolic Subdivision, Division of Internal Medicine, Dr. M. Djamil General Hospital/ Medical Faculty of Andalas University, Padang, West Sumatera, Indonesia
Elvira, Dwitya; Allergy and Immunology Subdivision, Division of Internal Medicine, Dr. M. Djamil General Hospital/ Medical Faculty of Andalas University, Padang, West Sumatera, Indonesia
Aprilia, Dinda; Endocrinology and Metabolic Subdivision, Division of Internal Medicine, Dr. M. Djamil General Hospital/ Medical Faculty of Andalas University, Padang, West Sumatera, Indonesia
TRH, TSH, T-reg, IL-4, anti-TPO
The most common cause of hyperthyroidism is Graves' disease. TRH and TSH are hormonal factors that modulate and control thyroid function in Graves' disease. In the immunological aspect, Graves' disease is played by the role of T-reg, IL-4, and anti-TPO. Graves' disease treatment goal is to inhibit thyroid hormone secretion by administering thionamide. The evaluation of this treatment is its hormonal and immunological aspects. To describe the effect of thionamide on serum TRH, TSH, IL-4, T-reg, and anti-TPO levels in Graves' disease. This study is a clinical trial study in 25 study participants. All study participants were given thionamide, namely PTU 300mg for three months and blood samples were taken for laboratory tests. Serum TRH, TSH, IL-4, T-reg FOXP3, and anti-TPO levels were examined by ELISA. The mean levels at the beginning and after three months of therapy are: serum TRH 92.589pg/mL and 115.944pg/mL; serum TSH 0.041mU/L and 0.223mU/L; serum IL-4 19.759pg/mL and 23.040pg/mL; T-reg FOXP3 gene polymorphism 0.621ng/mL and 0.518 ng/mL; serum anti-TPO 2697.539pg/mL and 2604.710pg/mL. Increased levels of serum TRH and TSH levels were statistically significant. The change in serum IL-4, T-reg FOXP3 gene polymorphism, and anti-TPO levels were not statistically significant. The administration of thionamide in Graves' disease for three months will significantly decrease Wayne index and serum FT4 levels, increase serum TRH and TSH levels.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-05-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1521
10.14499/indonesianjpharm30iss2pp122-127
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 122-127
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1521/897
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/512
2018-09-05T01:49:54Z
3:ART
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/675
2020-01-27T02:53:16Z
3:ART
ISOLATION AND IDENTIFICATION OF FLAVONOIDS FROM Sesamum indicum
Sharma, Priyanka; Laboratory of Bioactive Compounds and
Algal Biotechnology Department of Botany,University of Rajasthan, Jaipur- India.
302004
Sarin, Renu; Laboratory of Bioactive Compounds and
Algal Biotechnology Department of Botany,University of Rajasthan, Jaipur- India.
302004
Natural substances have long served as sources of therapeutic drugs. Many substances have been derived from traditional medicines. The plants are rich in secondary metabolites. The medicinal properties of these plants have been attributed to the biochemicals present in the plant materials. In addition to their role in human and animal nutrition, knowledge of micronutrients and phytochemical composition is fundamental to the understanding of modes and mechanisms of action of medicinal plants in general. In the present investigation, quercetin and kaempferol have been isolated and identified from stem, leaves and unorganized cultures of Sesamum indicum and maintained by frequent subculturings on Murashige and Skoog’s medium (1962) supplemented with NAA+BAP(5.0+0.5mg/L). The study showed that maximum content of quercetin and kaempferol was observed in 6 weeks old calli and minimum in stem of S. indicum. The structure of the isolated compound was established on the basis of physical, chemical test and spectroscopic evidences.Key words: Flavonoids, quercetin, kaempferol, Sesamum indicum
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/675
10.14499/indonesianjpharm23iss3pp135-140
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 135-140
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/675/541
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1468
2019-10-28T01:20:02Z
3:ART
Antibacterial Compound from Aspergillus elegans SweF9 an Endophytic Fungus from Macroalgae Euchema sp.
Mulyani, Hani; 1. Pharmacy Faculty, Pancasila University,Jl. Raya Lenteng Agung Timur No.56-80, Srengseng Sawah, Jagakarsa, Jakarta
2. Indonesian Institute of Sciences (LIPI)
Dewi, Rizna Triana; Indonesian Institute of Sciences (LIPI)
Chaidir, Chaidir; Pharmacy Faculty, Pancasila University,Jl. Raya Lenteng Agung Timur No.56-80, Srengseng Sawah, Jagakarsa, Jakarta
Aspergillus elegans SweF9, antibacteria, (+)-epi-Epoformin.
The antibiotic resistance of bacterial pathogens has become a serious health concern and encouragement to search for novel and efficient antimicrobial metabolites. On the other hand, endophytic fungi have great potential as a natural source for antimicrobial agents. The objective of this study was to isolation antibacterial compound from endophytic fungi of A.elegans SweF9. The fungus was stationarily cultured at 30°C for 12 days in potato malt peptone (PMP) medium, then extracted with ethyl acetate. The antibacterial activities of the extract were evaluated by agar well diffusion method against Gram-poitive (Bacillus subtilis and Staphylococcus aureus) and Gram-negative (Escherichia coli) bacterial strains. The broth extract was able to inhibit the growth of E. coli, S. aureus and B. subtilis with antibacterial activity index compared to streptomycin sulfate were 84.6%, 91.6%, and 90% respectively. The active compound (1) was purified to yield amorphous white and identified using FTIR, NMR, and EI-MS analyses, revealed identified as (+) - epi-Epoformin. The compound showed an antibacterial activity index of E. coli, S. aureus and B. subtilis bacterial were 38%, 45%, and 47%, respectively. Based on these results endophytic fungi A. elegans SweF9 can be used as a new source of potential antibacterial compounds
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Indonesian Institute of Sciences
2019-09-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1468
10.14499/indonesianjpharm30iss3pp217
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 217
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1468/916
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/659
2020-01-27T02:53:58Z
3:ART
FORMULATION OF NANOCURCUMIN USING LOW VISCOSITY CHITOSAN POLYMER AND ITS CELLULAR UPTAKE STUDY INTO T47D CELLS
Chabib, Lutfi; Departement of Pharmacy, Faculty of Mathematics and science Universitas Islam
Indonesia 55584, Yogyakarta
Martien, Ronny; Departement of Pharmaceutics, Faculty of
Pharmacy, Universitas Gadjah Mada,
Yogyakarta, Sekip Utara Yogyakarta 55281, Indonesia
Ismail, Hilda; Departement of pharmaceutical Chemistry,
Faculty of Pharmacy, Universitas Gadjah Mada,
Sekip Utara Yogyakarta 55281, Indonesia
Using of curcumin as anti cancer agent is restricted by its low solubility, therefore it has low bioavability. This obstacle can be solved by the development of curcumin nanoparticle. Nanoparticle technology has been started to be developed as an alternative solution to improve drug delivery pofile, especially for the less bio-available chemical. This study was aimed to develope nanocurcumin formulation with low viscosity chitosan as the matrix and to study its ability to be taken into the cells in vitro. Method used in the formulation of nanocurcumin in this study is by ionic gelation followed by freeze drying. Entrapment Efficiency then assayed, and its stability was tested by incubating the formula into artificial intestinal fluid (AIF). Furthermore, its toxicity was evaluated, also its cellular uptake ability into T47D cell line. It was found that the Entrapment Efficiency in acetate buffer at pH 4 is higher than at pH 5. This formula also has a good stability in AIF. For the cellular uptake study through fluorescence microscope, it was found that the complex has an ability to penetrate cellular membrane into the cytosol. The cytotoxicity study tell us that the nanocurcumin is non-toxic to normal cell line. For the characterization of the nanoparticles, the average size of this particle is 269.8 nm, its zeta-potential is +18.63 mV, with spherical particle morphology. From the result ofthis study, it is concluded that formulation of nanocurcumin using low viscosity chitosan polymer as the matrix has a great potential as an alternative for anticancer therapy.Key words: nanoparticle, curcumin, low viscosity chitosan, T47D cell line.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/659
10.14499/indonesianjpharm23iss1pp27-35
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 27-35
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/659/525
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/689
2020-01-27T02:53:31Z
3:ART
PHYTOCHEMICAL STUDIES OF Acacia catechu
Baluja, Shipra; Department of Chemistry, Saurashtra University, Rajkot-360005 (Gujarat), INDIA
Chanda, Sumitra; Department of Biosciences, Saurashtra University, Rajkot-360005 (Gujarat), INDIA.
Solanki, Asif; Department of Biosciences, Saurashtra University, Rajkot-360005 (Gujarat), INDIA.
Kachhadia, Nikunj; Department of Biosciences, Saurashtra University, Rajkot-360005 (Gujarat), INDIA.
Acacia catechu Willd. belongs to the family Mimosaceae. It is well known for the powder of wood known as Kathha” in India, which has wide applications. In the present work, the dry wood powder is extracted in various solvents such as 1,4-dioxane, methanol, dimethyl formamide (DMF), acetone, ethanol and distilled water. The phytochemical and physicochemical properties of these various solvent extracts were studied. The phyto constituents present in the different extracts were saponins, tannins, sugars, terpenes, catechu-tannic acid. Alkaloids were present in organic solvents only. The antibacterial activity of all the extracts in DMSO was studied against five bacterial strains but none of the extracts inhibit any of the studied bacterial strains.Key words: Acacia catechu, 1,4-dioxane, methanol, dimethyl formamide (DMF), acetone, ethanol, distilled water, phytochemical analysis, physicochemical analysis.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/689
10.14499/indonesianjpharm23iss4pp238-247
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 238-247
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/689/555
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1388
2019-10-03T03:16:50Z
3:ART
Screening of Antibacterial and Anticancer Activity of Soft Corals from Togean Islands, Indonesia
Zubair, Muhammad Sulaiman; Department of Pharmacy, Faculty of Sciences, Tadulako University, Kampus Bumi Tadulako, 94118, Palu, Indonesia
Lallo, Subehan; Faculty of Pharmacy, Hasanuddin University, 90245, Makassar, Indonesia
Rusmiyanti, Rusmiyanti; Department of Pharmacy, Faculty of Sciences, Tadulako University, Kampus Bumi Tadulako, 94118, Palu, Indonesia
Nugrahani, Arsa Wahyu; Department of Pharmacy, Faculty of Sciences, Tadulako University, Kampus Bumi Tadulako, 94118, Palu, Indonesia;
Jantan, Ibrahim; School of Pharmacy, Taylor’s University, Lakeside Campus, 1 Jalan Taylors, 47500 Subang Jaya, Selangor, Malaysia
Softcorals, Togean Islands, anti-cancer, antibacterial
Soft corals (Octocorallia, Alcyonaceae) have been reported to possess diverse biological activities and unique structural chemistry. This study aims to screen the potential antibacterial and anticancer activity of some soft corals collected from Togean Islands, Central Sulawesi, Indonesia. They were Lobophytum sp, Sarcophyton sp, Sinularia sp 1, and Sinularia sp 2. All dried coral materials were extracted for 3 x 24 h by maceration method using methanol and then evaporated by rotary evaporator to obtain viscous extracts. The determination of antibacterial activity had been performed by well agar diffusion method against Staphylococcus aureus and Escherichia coli. Meanwhile, the cytotoxic activity was performed by MTT method, followed by apoptosis annexin V-FTIC assay agains. Identification for the presence of terpenoids was performed by vacuum p-anisaldehyde-sulphuric acid spraying reagent on thin layer chromatography (TLC). Sinularia sp2 extract have strongly inhibited S. aureus and E.coli with the diameter of inhibition range from 12.76mm and 17.86mm, respectively. Moreover, Sinularia sp2 extract possessed also cytotoxic activity against human breast adenocarcinoma (MCF-7) and colorectal carcinoma (HCT-116) with the IC50 of 46.807 and 47.186 μg/mL, respectively. Extract Sinularia sp 1 was found to have strongest cytotoxicity on human colon colorectal carcinoma (HCT-116) with the IC50 of < 1.505 μg/mL. Annexin V-FTIC assay clearly exhibited that the apoptosis mechanism is proposed by the extracts of Sinularia sp1 and Sinularia sp 2. Terpenoids were identified on both extracts suggesting for further purification and isolation for the bioactive terpenoid compounds.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-11-21
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1388
10.14499/indonesianjpharm29iss4pp173
Indonesian Journal of Pharmacy; Vol 29 No 4, 2018; 173
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1388/880
Copyright (c) 2018 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1237
2020-03-18T10:32:19Z
3:ART
Analysis of Factors Influencing the Availability of Medicine During JKN Era
Satibi, Satibi; Faculty of Pharmacy, Universitas Gadjah Mada
Ranowijaya, Ranowijaya; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
Aswandi, Aswandi; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
Bermalam, Junagsti; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
Widodo, Gunawan Pamudji; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
JKN; availability of drugs factor; doctors factor; pharmacists factor and patients factor
The availability of medicine is important during National Social Healthy Insurance (JKN) that put into effect in the hospital, one of health service facility. The purpose of this study was to look of the availability of medicines was during JKN era and what factors influence the availability in three Region General Hospitals in Sulawesi. This study was conducted using descriptive method of analytic to get a correlation among doctors, pharmacists and patients as independent variables toward the availability of medicine as dependent variable. The study has been performed in 3 hospitals in Sulawesi. This data obtained were analyzed using statistical model of Linier Regression Analysis with F test or ANOVA test and t test. R was the value of regression which showed the influence of one to another variable. The results showed the level of medicine availability in hospital A was 12.49 months. Factors that influence the availability of medicines were doctors (R=0.778, p= 0.000), pharmacists (R=0.619, p= 0.000) and patients (R=0.653, p= 0.000). All there factor significantly affect the availability of medicines in Hospital A (R=0.675, p=0.000). Hospital B had an excess supply of medicines, with the average of 23.31 month. Factors that influenced the availability of medicines in Hospital B was doctor (R=0.877), pharmacist (R=0.861) and patient (R=0.984) (p=0.000 all). From multiple regression there factors showed a significant correlation to the availability of medicines (R=0.901, p=0.000). Similarity on Hospital A and B, data of the availability of medicine in hospital C was categorized excess as the average of stock was 56.01 month. Factors that influenced the availability of medicines in Hospital C was doctor (R=0.753, p= 0.000), pharmacist (R=0.869, p= 0.000) and patient (R=0.545, p=0.001).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-03-12
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1237
10.14499/indonesianjpharm29iss1pp37
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 37
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1237/837
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1505
2019-10-10T01:29:33Z
3:ART
In Vitro Study of the Combination of Doxorubicin, Curcuma xanthorrhiza, Brucea javanica, and Ficus septica as a Potential Novel Therapy for Metastatic Breast Cancer
Sutejo, Ika Rahmawati; Biochemistry Department, Faculty of Medicine, University of Jember, Jalan Kalimantan 37 Jember, Indonesia
Cancer Chemoprevention Research Center, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Putri, Herwandhani; Cancer Chemoprevention Research Center, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Handayani, Sri; Cancer Chemoprevention Research Center, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Research Center for Chemistry, Indonesian Institute of Sciences (LIPI), Gedung 452 Kawasan PUSPIPTEK Serpong, Tangerang Selatan, Banten 15314, Indonesia
Jenie, Riris Istighfari; Department of Pharmaceutical Chemistry Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Cancer Chemoprevention Research Center, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Meiyanto, Edy; Professor of Pharmaceutical chemistry, Faculty of Pharmacy. Universitas Gadjah Mada
C xanthorrhiza, Bjavanica, F septica, antimetastasis, 4T1 cells
Less optimized therapeutic effects constrain the use of doxorubicin as the main agent of chemotherapy for metastatic breast cancer, resistance and side effects. Therefore we need a combination of more than one chemopreventive agent which has different molecular targets to solve that problem. The aims of this study is to prove the inhibitory effect of ethanolic extract of rhizome of Curcuma xanthorrhiza (ECx), fruit of Brucea javanica (EBj), leave of Ficus septica (EFs) and doxorubicin (Dox) alone and its combination on migration and invasion of a highly metastatic 4T1 breast cancer cell line. Cytotoxic activity of single and combination treatment was evaluated by MTT assay, followed by an experiment of apoptosis induction by using flow cytometry. The inhibitory effect on migration was observed by the scratch wound-healing assay. Furthermore, the observation of the activity of matrix metalloproteinase-9 (MMP-9) was analyzed by gelatin zymography. The results showed that ECx, EBj, EFs, and Dox has cytotoxic activity on 4T1 cells with the value of IC50 respectively 49.7±1.53mg/mL, 59.9±1.79mg/mL, 15.2±2.12mg/mL and 1.2±0.23mM. Furthermore, combination of ECx-EBj-Dox and ECx-EBj-EFs revealed synergistic effect on 4T1 cells and decrease cell viability through the induction of apoptosis and necrosis. Based on wound healing assay, 24 hours incubation of this combination inhibited 4T1 cells migration compared to single treatment. Gelatin zymography analysis showed that this combination also inhibited the activity of MMP-9 greater than a single use. Curcuma xanthorrhiza, Brucea javanica, and Ficus septica may have potential to be developed as a combination with or without doxorubicin for metastatic breast cancer treatment.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1505
10.14499/indonesianjpharm30iss1pp15
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 15
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1505/889
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1493
2021-01-07T01:00:39Z
3:ART
THE EFFECT OF GIVING DELTAMETHRIN AND CYPERMETHRIN INSECTICIDES ON THE LEVEL OF INTRATESTICULAR TESTOSTERONE IN MALE WISTAR STRAIN RATS
Triutomo, Devyanto Hadi; Faculty of Pharmacy UGM
Puspitasari, Ika; Faculty of Pharmacy, Universitas Gadjah Mada
Susidarti, Ratna Asmah; Faculty of Pharmacy, UGM
cypermethrin, deltamethrin, intratesticular testosterone
The use of deltamethrin and cypermethrin insecticides to control pests has been implemented in Indonesia. Irregular and excessive use of insecticides can have toxic effects on the male reproduction system. Deltamethrin and cypermethrin insecticides can inhibit androgen biosynthesis and disrupt the growth of sperm. Therefore, this research is conducted to find out the effect of deltamethrin and cypermethrin insecticides toward the level of intratesticular testosterone in male Wistar strain rats. This research is conducted in vivo using male Wistar strain rats. Nine rats are divided into three groups of treatment, namely control group, deltamethrin group with 0.26 mg/kg of weight, and cypermethrin group with 0.26 mg/kg of weight. Treatment is conducted every day for 15 days orally, and then the rats are dissected to take their testicles. Testicles are chopped and their intratesticular homogenates are taken by adding the medium of DMEM and collagenase (0.25 mg/mL). The measurement of the level of testosterone is conducted by using Electrochemiluminescence immunoassay (ECLIA) method. Data on the change of the increase of body weight, organ weight, and the level of testosterone is analyzed statistically using the one-way ANOVA test. The research result shows that giving 0.26 mg/kg of weight of deltamethrin and 0.26 mg/kg of weight of cypermethrin does not affect the increase in body weight, relative weight of the reproductive organ, and the level of intratesticular testosterone of male Wistar strain rats.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-08-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1493
10.14499/indonesianjpharm31iss2pp116
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 116
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1493/947
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/667
2020-01-27T02:51:49Z
3:ART
THE OPTIMISED CONDITIONS OF INDUCTION OF RECOMBINANT RIP rMJC15310 ACTIVITY ISOLATED FROM Mirabilis jalapa L. LEAVES
Astuti, Puji; Department of Pharmaceutical Biology,
Faculty of Pharmacy Universitas Gadjah Mada,
Sekip Utara, Yogyakarta, 55281.
., Sudjadi; Department of Pharmaceutical Chemistry, Faculty of Pharmacy Universitas Gadjah Mada, Sekip Utara, Yogyakarta, 55281
., Sismindari; Department of Pharmaceutical Chemistry, Faculty of Pharmacy Universitas Gadjah Mada, Sekip Utara, Yogyakarta, 55281
Ribosome Inactivating Proteins (RIPs) are compounds isolated from plants with ability to inhibit protein synthesis. The inhibition of protein synthesis is due to inactivation of ribosomal RNA through a site-specific deadenylation mediated by RNA N-glycosidase. Reportedly, RIPs mainly possess wide range of bioactivity including antiviral activity against plant infections. Other activities of RIP were as abortifacien, antivirus and anticancer. This study was aimed to isolate and characterize the optimum conditions for inducing the expression of recombinant RIPs isolated from the leaves of Mirabilis Jalapa L. We have been successfully isolated several RIPs and engineered these proteins to be expressed in E. coli. These recombinant proteins were obtained by screening cDNA library originated from the mRNA of Mirabilis jalapa L leaves, and inserted into pUC19 carrying lacZ gene. The presence of recombinant plasmid was tested by using α-complementation assay. Many RIPs have been isolated from plants and these proteins express enzymatic activity by cutting supercoiled double stranded DNA. One RIP namely rMJC15310 was obtained from this study and the proteins having ~ 8kb in size, cut the supercoiled DNA into linear form at the concentration as low as 5 µg. The ability to cut supercoiled DNA increased on inducing its expression with 0.4% IPTG.Key words: Ribosome Inactivating Proteins (RIP), IPTG, Mirabilis jalapa L., recombinant protein
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/667
10.14499/indonesianjpharm23iss2pp93-98
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 93-98
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/667/533
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1357
2018-11-02T06:46:53Z
3:ART
Pharmaceutical applications of Aloe vera
Heng, Huay Chin; Faculty of Pharmacy, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia
Zulfakar, Mohd Hanif; Centre for Drug Delivery Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia
Ng, Pei Yuen; Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia,
50300 Kuala Lumpur, Malaysia
A. vera; anti-diabetic; anti-inflammatory; wound healing; antibacterial
Aloe vera has been used as folk medicine for a host of therapeutic indications of which the inner gel is the component extensively used and studied. Proponents of the use of this plant suggest that it is easily available, economical, and have fewer side effects compared to commercial drug compounds. However, the active constituents and their exact mechanisms have yet to be fully elucidated. This review focuses on the identification of the active constituents and their functional mechanism in the areas of anti-diabetic, anti-inflammatory, wound healing, and antibacterial. Preliminary evidence was found to support the antidiabetic effect which extended to the early stages of the disease with no adverse effects at the dosages used. Although topical application for its anti-inflammatory effect may be delayed and minute, oral administration has shown a significant response. However, too high a dose has been linked with an initial inflammatory reaction. Favorable response to the gel has also been demonstrated to support the use of A. vera in wound-healing and as an antibacterial agent, although limited to simple, uncomplicated wounds. Different active constituents such as acemannan and aloin have been suggested for the effects of the plant and this may be interpreted as synergism among different compounds rather than the action of a single compound. Until robust evidence is available, the plant should only be used as an adjunct to other well-established evidence-based treatment modalities.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Ministry of Education (FRGS/1/2016/SKK09/UKM/02/4)
2018-09-24
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1357
10.14499/indonesianjpharm29iss3pp101
Indonesian Journal of Pharmacy; Vol 29 No 3, 2018; 101
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1357/872
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1519
2019-10-28T01:20:02Z
3:ART
A Study of Psychoactive Medicines and Risk of Falls Among Indonesian Elderly Patients
Rahmawati, Fita; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia 55281
Mustafidah, Nasikhatul; Postgraduate Magister of Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia 55281
Department of Pharmacy RSUD. Dr. Soedono Madiun
Pramantara, I Dewa Putu; Department of Geriatric, RSUP Dr. Sardjito Hospital, Yogyakarta, Indonesia 55281
Wahab, Izyan Abdul; Faculty of Pharmacy, Cyberjaya University College of Medical Sciences, Persiaran Bestari, Cyber 11, 63000 Cyberjaya, Selangor, Malaysia
Psychoactive medicine, Fall risk, Elderly
One of the causes of injury to the elderly is due to falls. Falling can be prevented by identifying and controlling risk factors. One risk factor that can be controlled is the use of fall risk medicines including psychoactive. This study aims to identify the association between the use of psychoactive medicine and its characteristic with the risk of falls among the elderly in Indonesia. The study utilized a case-control study design for a total number of 414 elderly patients, during October until December 2018. Cases were elderly aged 60 years or above with a high risk of falling assessed using the Morse Fall Scale (MFS≥45). Each case was matched with up to two randomly selected controls of the same age who are classified as low to moderate risk of falling (MFS<45). The use of psychoactive medicines was screened from a history of drug use for the past six months. Psychoactive medicine-fall risk associations were estimated via logistic regression. There were 138 cases and 276 controls. The median age of subjects was 66 years old and 54.83% was a woman. Elderly with a high risk of falling had higher psychoactive medicines use when compare with controls (31.16 % vs 21.38 %, p< 0.05). After adjusting for potential confounders, the use of psychoactive medicines was significantly associated with higher fall risk in elderly patients (OR 1.79 95% CI 1.10-2.90). Only the duration of psychoactive medication use over 90 days was significantly associated with a high risk of falling (AOR 3.65 95% CI 1.46-9.14). In elderly patients, the continued use of psychoactive medicines increased the risk of fall. Prescribers need to weigh risk and benefit from the use of psychoactive medicines in the elderly to prevent future fall.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Faculty of Pharmacy Universitas Gadjah Mada
2019-07-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1519
10.14499/indonesianjpharm30iss3pp233
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 233
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1519/907
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/684
2020-01-27T02:53:31Z
3:ART
PHYSICAL PROPERTIES OF WOUND HEALING GEL OF ETHANOLIC EXTRACT OF BINAHONG (Anredera cordifolia (Ten) Steenis) DURING STORAGE.
Yuliani, Sri Hartati; Faculty of Pharmacy, Universitas Gadjah Mada,
Skip Utara, Yogyakarta 55281 Indonesia
Faculty of Pharmacy, Universitas Sanata Dharma, Paingan, Maguwoharjo, Depok, Yogyakarta 55284 Indonesia
Fudholi, Achmad; Faculty of Pharmacy, Universitas Gadjah Mada,
Skip Utara, Yogyakarta 55281 Indonesia
Pramono, Suwijiyo; Faculty of Pharmacy, Universitas Gadjah Mada,
Skip Utara, Yogyakarta 55281 Indonesia
., Marchaban; Faculty of Pharmacy, Universitas Gadjah Mada,
Skip Utara, Yogyakarta 55281 Indonesia
Binahong (Anredera cordifolia (Ten) Steenis) has been used as wound healing in traditional Indonesian medicine. The developing of the dosage forms using the formulation technology approach has been done. The aim of this study was to examine the physical properties of the wound healing gel of ethanolic extract of binahong during storage. The factorial design method 3 factors and 2 levels was employed to achieve this study. The three factors used in this study were Carbopol, CMC-Na and Caalginate with low and high level for each factor. The physical properties of the wound healing gel of ethanolic extract of binahong was evaluated on day 1, 30, 60 and 90 for viscosity and bioadhesion study. The result showed that CMC-Na and Caalginate increased the alteration of physical properties of wound healing gel of ethanolic extract of binahong during storage. The carbopol maintained the physical properties of the gel during storage.Key words: bioadhesion, binahong, viscosity, wound healing gel.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/684
10.14499/indonesianjpharm23iss4pp203-208
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 203-208
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/684/550
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1410
2022-10-26T01:05:53Z
3:ART
In Vitro Study: Effect of Cobalt(II) Chloride Against Dengue Virus Type 1 in Vero Cells
Sucipto, Teguh Hari; Institute of Tropical Disease, Universitas Airlangga
Untoro, Yovilianda Maulitiva; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga
Setyawati, Harsasi; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga
Maharani, Anisa; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga
Sholihah, Novia Faridatus; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga
Churrotin, Siti; Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga
Amarullah, Ilham Harlan; Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga
Soegijanto, Soegeng; Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga
Cobalt(II) chloride, DENV-1, and Antiviral activity.
Dengue virus (DENV) serotypes DENV-1 to DENV-4 are enveloped viruses that belong to the genus Flavivirus of the Flaviviridae. Dengue vaccine or antiviral has not yet been clinically approved for humans, even though there have been great efforts toward this end. Antiviral activity against DENV is needed to develop to be an alternative drug for DENV virus. Cobalt(II) chloride have been used in the treatment and prevention of diseases of humans since ancient times. The aim of this study is to investigate the antiviral effects and Cytotoxicity of Cobalt(II) chloride. This compound was further investigated for its inhibitory effect on the replication of DENV-1 in Vero cells. Antiviral activity and Cytotoxicity measured by WST-1 assay. The IC50 value of the Cobalt(II) chloride for DENV-1 was 0.38 μg/ml. The cytotoxicity of Cobalt(II) chloride to Vero cell suggest that the CC50 value was 2.91 µg/ml The results of this study demonstrate the anti-dengue serotype 1 inhibitory activity of Cobalt(II) chloride was a high toxic compound.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-01-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1410
10.14499/indonesianjpharm30iss4pp316
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 316
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1410/931
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/507
2020-02-19T02:37:07Z
3:ART
SEDATIVE EFFECT OF CLARY SAGE (SALVIA SCLALAREA, L) ON MICE USING AN OLFACTORY AROMATHERAPY
Hadinoto, Idajani; Faculty of Pharmacy
Unika Widyamandala Surabaya
Kuswono, Engkun; Faculty of Pharmacy
Unika Widyamandala Surabaya
Marlina, Ani; Faculty of Pharmacy
Unika Widyamandala Surabaya
Setiawati, Anna; Faculty of Pharmacy
Unika Widyamandala Surabaya
A research has been conducted to study the sedative effect of the Clary sage oil (Salvia sclaria, L), using an olfactory aromatherapy method, on male white mice, which have been tested using rotarod, platform and chimney tests. Clary sage oil was given to the male white mice in three different exposed times : 0.5, 1, and 1.5 hours. As a reference standard was used diazepam 170 ug/kg body weight (intraperitoneal). The research indicates that the administration of Clary sage oil can indeed cause the sedative effect. There is also a correlation between the exposure time of Clary sage oil and the sedative effect observed. Key-words : sedative effect, clary sage oil, olfactory aromatherapy
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/507
10.14499/indonesianjpharm0iss0pp44-51
Indonesian Journal of Pharmacy; Vol 12 No 1, 2001; 44-51
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/507/385
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1334
2019-10-10T01:29:33Z
3:ART
Profile of Biofilm-Producing Staphylococcus epidermidis from Intravenous Catheter Colonisation at Prof. Dr. Margono Soekarjo Hospital Purwokerto
Anjarwati, Dwi Utami; Jenderal Soedirman University
Siswandari, Wahyu; Dept of Pathology Clinic, Medical Faculty of Jenderal Soedirman University-Margono Soekarjo Hospital, Dr Gumbreg Nomor I, Medical Street, Mersi, Purwokerto, 53112
Peramiarti, IDSAP; Dept of Microbiology, Medical Faculty of Jenderal Soedirman University, Dr Gumbreg Nomor I, Medical Street, Mersi, Purwokerto, 53112
Biofilm, Intravenous catheter, colonization, Staphylococcus epidermidis
Biofilm- producing Staphylococcus epidermidis has evolved to be a significant human pathogen, particularly in the use of medical devices such as an intravenous catheter. Furthermore, biofilm-producing bacteria 10-1000 fold less susceptible to several antimicrobial agents than free-bacteria. This simple survey aimed to describe the profile of biofilm-producing S. epidermidis from intravenous catheter colonization of some patients in surgical and internal medicine wards at the hospital Margono Soekarjo, Purwokerto, and the antibiotics resistance pattern. A vitek® 2 compact (Enseval Medika Prima) was performed to identify the bacterial species and to examine the 73 antibiotics for understanding the resistance pattern automatically. Microtiter plate biofilm assay with crystal violet staining was performed to measure biofilm optical density (OD) for analyzing the biofilm production capabilities. A scanning electron microscopy (SEM) was done to compare the thickness of ultrastructure of biofilm-producing S. epidermidis visually. The present study found that 2 of 8 Gram-positive bacteria (25%) were biofilm-producing S. epidermidis. One of S. epidermidis was moderate whereas the other was high biofilm-producing bacteria. Images of SEM showed that a high biofilm-producing S. epidermidis has a thicker ultrastructure of biofilm than the moderate biofilm-producing, whereas a control, the weak biofilm-producing S. epidermidis ATCC 12228 has the least biofilm. Both of S. epidermidis strains were sensitive to Gentamicin, Moxifloxacin, Quinupristin/Dalfopristin, Linezolid, Vancomycin, Doxycycline, Minocycline, Tetracycline, Tigecycline, and Nitrofurantoin. Furthermore, both S. epidermidis strains were resistant to the other (63) antibiotics. In conclusion, two strains of S. epidermidis in this study have different capabilities to form the biofilm which were showed that high biofilm-producing strain was thicker than moderate biofilm-producing strain by scanning electron microscopy. However, both of them were resistant to the same number of antibiotics.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Prof. dr. Margono Soekarjo Hospital, Purwokerto
Research and higher education ministry, Indonesia
2019-03-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1334
10.14499/indonesianjpharm30iss1pp1
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 1
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1334/887
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1820
2021-01-06T13:13:53Z
3:ART
The effect of combination of active fraction Andrographis paniculata (Burm.f) Ness and Centella asiatica (i) Urban on the alpha glucocidase inhibitor and antioxidant activities
Rusman, Arman; Master program, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
Nugroho, Agung Endro; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281
Pramono, Suwidjiyo; Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281
The Andrographis panicullata and Centella asistica extract have been reported that had a anti-diabetic effect. However, the specific mechanism and the effect combination of both were not yet reported. This study was purposed to determine the potency of extract, fractions and the combination of Andrographis panicullata (AP) and Centella asistica (CA) active fraction to inhibit alpha glucosidase enzymeand its ability to reduce DPPH radical.AP and CA were extracted using 50% ethanol then fractionated with solvents under different polarity levels. The inhibiting activity to alpha glucosidase enzyme and antioxidant activity of each fractions was tested. The most active fractions from AP and CA were then combined and re-tested for activity. The results result reported that both of AP and CA had inhibition of alpha glucosidase activity and antioxidant activity. Based on calculation combination index (CI) of of active fraction of AP and CA showing in alpha glucocidase activity had a antagonist action and antioxidant had a sinergic action. Therefore, combination of AP and CA not has not recomended for alpha glucocidase inhibitor but the combination has ability to reduce DPPH radical.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-04-09
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1820
10.14499/indonesianjpharm31iss1pp56
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 56
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1820/945
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1820/297
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/662
2020-01-27T02:53:58Z
3:ART
SELAGINELLA ACTIVE FRACTIONS INDUCE APOPTOSIS ON T47D BREAST CANCER CELL
Handayani, Sri; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Risdian, Chandra; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Meiyanto, Edy; Faculty of Pharmacy, Universitas Gadjah
Mada, Indonesia. Skip Utara 55281
Yogyakarta
Udin, Zalinar; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Andriyani, Rina; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Angelina, Marissa; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Apoptosis is an important target on anticancer mechanism. The purpose of this research is to investigate apoptosis induction of Selaginella plana Hieron active fractions on T47D cells. Absolute ethanol was used to extract Selaginella plana powders. Ethanolic extract was dilluted by methanol:water (4:1) and then fractionated by hexane (S_Hex), methylene chloride (S_MTC), ethyl acetate (S_EA), and buthanol (S_BuOH). The proliferation of T47D cell line was detected by SRB (Sulforhodamine B) assay which was measured at a wavelength of 515nm. Flowcytometry analysis to determine apoptosis was examined by Propidium Iodide (PI) and Annexin V assay using T47D breast cancer cell line. The result showed that the IC50 value of S_Hex, S_MTC, S_EA, and S_BuOH on T47D cells were 107 µg/mL, 4 µg/mL, 6 µg/mL, and 17 µg/mL respectively. The active fractions (S_MTC and S_EA) at its IC50 concentration significantly (P<0.05) increased the total number of early apoptotic cells in the T47D cells 3.39% and 4.1% respectively compared to that of control (1.95%). Based on the result, methylene chloride and ethyl acetate fraction of Selaginella plana induced apoptosis on T47D cell.Keywords: apoptosis, breast cancer, Selaginella
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/662
10.14499/indonesianjpharm23iss1pp48-53
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 48-53
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/662/528
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1323
2018-10-23T02:19:33Z
3:ART
Isolation and Identification of α-Glucosidase Inhibitor From Aspergillus Terreus F38
Munasaroh, Siti; Universitas Pancasila
Tamat, Swasono R; Pancasila University
Dewi, Rizna Triana; Chemistry Research Center – LIPI
α-glukosidase inhibition, Aspergillus terreus F38, butyrolactone III.
Controlled postprandial glucose level is an important strategy in preventing DM type 2. Inhibitors of α-glucosidase have been postulated to be useful agents in managements of DM type 2. This research aims to isolate and identify of α-glucosidase inhibitor fromAspergillus terreus F38 by liquid fermentation. The mycelium extract of A. terreus F38showed strong activity against α-glucosidase with IC50 value of 9.65 μg/mL. Separation and purification of mycelium ectract yielded compound I (Butyrolactone III). The structure was establish on the basis of spectral analysis, according to the data obtained by NMR and LCMS-MS experiments. Compound I showed potential activity against α-glucosidase with IC50 value of 13.87 μg/mL. Therefore, the metabolites from A. terreus F38 can be used as lead compound to design potent α-glucosidase inhibitory agents.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-07-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1323
10.14499/indonesianjpharm29iss2pp74
Indonesian Journal of Pharmacy; Vol 29 No 2, 2018; 74
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1323/869
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1495
2019-10-10T01:30:23Z
3:ART
Evaluation of Pain Scale Decrease and Adverse Effects of Ketorolac Injections: An Observational Study in Patients with Postoperative Pain
Ihsan, Mawardi; Department of Pharmacology and Clinical Pharmacy, Universitas Gadjah Mada
Kurniawati, Fivy; Department of Pharmacology and Clinical Pharmacy, Universitas Gadjah Mada
Khoirunnisa, Husna; Undergraduate Program of Pharmacy, Universitas Gadjah Mada
Chairini, Belladonna; Undergraduate Program of Pharmacy, Universitas Gadjah Mada
The use of ketorolac injections in Indonesia is restricted with the provision of 2-3 ampoules per day with a maximum of two days even though the literature states that ketorolac could be used for no more than five days. This study aimed to determine the decrease in pain scale as well as gastrointestinal and renal adverse effects of ketorolac injections in two days of use. This study was an observational study with one-group pre-test post-test design conducted prospectively. The group was a group of patients with postoperative pain who received ketorolac injections and were treated during January till April 2018 in an academic hospital in Yogyakarta. The results showed that ketorolac injections did not provide a statistically significant decrease in pain scale in two days of use compared to before surgery (median [range] = 2.0[0.0-9.33] vs 1.33[0.0-8.33]; p=0.32). Ketorolac injections decreased the kidney function of subjects in two days of use compared to before surgery based on creatinine values (0.76mg/dL vs 0.80mg/dL; p=0.024) and GFR (96.13mL/min/m2 vs 87.52mL/min/m2; p=0.023), and as many as 31 subjects (43.06%) experienced complaints that were suspected to be the gastrointestinal adverse effects of ketorolac injections with the three most complaints were bloating (18.06%), nausea (16.67%), and heartburn (15.28%). Those three results support the use of ketorolac injections following what has been regulated in the Indonesian National Formulary.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-05-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1495
10.14499/indonesianjpharm30iss2pp133-140
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 133-140
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1495/903
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/679
2020-01-27T02:53:16Z
3:ART
QSAR MODELING OF 2-[CH(OH)X]-5,8-(OY)2 -1,4-NAPHTHOQUININES AGAINST L1210 CELLS USING MULTIPLE LINEAR REGRESSION
., Ajeet; Department of Pharmaceutical Chemistry,
S. D. College of Pharmacy and Vocational Studies, Muzaffarnagar, U.P. 251001
India
Singh, Brajpal; Department of Pharmaceutical Chemistry,
S. D. College of Pharmacy and Vocational Studies, Muzaffarnagar, U.P. 251001
India
Kumar, Vipul; Department of Pharmaceutical Technology, Meerut Institute of Engineering and Technology, Meerut, U.P., India
Quinones are present in many drugs such as anthracyclines, daunorubicin, doxorubicin, mitomycin, mitoxantrones and saintopin, which are used clinically in the therapy of solid cancers. The cytotoxic effects of these quinone are mainly due to the inhibition of DNA topoisomerase-II. It is the necessity to develop the 1,4-Naphthoquinone analogues with Cytotoxic effect. Here 2-[CH(OH)X]-5,8-(OY)2-1,4-Naphthoquinines analogues have been used to correlate the cytotoxic activity with the Eccentric Connectivity index (ECI), Fragment Complexity (FC) and McGowan Volumes (MG) for studying the Quantitative Structure Activity Relationship (QSAR). Correlation may be an adequate predictive model which can help to provide guidance in designing and subsequently yielding greatly specific compounds that may have reduced side effects and improved pharmacological activities. We have used Multiple Linear Regression (MLR), one of the best methods for developing the QSAR model. Results from this QSAR study have suggested that ECI, FC and MG are the important descriptors for cytotoxic activities of 1,4-Naphthoquinones against L1210 cells. For the validation of the developed QSAR model, statistical analysis such as data point-descriptor ratio, fraction of variance, cross validation test, standard deviation, quality factor, Fischer’s test; and internal validation such as Y-randomization test have been performed and all the tests validated this QSAR model.Key words: 1,4-Naphthoquinones, QSAR, Eccentric connectivity index, Fragment complexity, McGowan Volume, Multiple Linear Regression
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/679
10.14499/indonesianjpharm23iss3pp171-176
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 171-176
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/679/545
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1403
2022-10-26T01:05:53Z
3:ART
The capability of Several Population-based Approach Software to Analyze Sparse Drug Plasma Concentration Data after Intra-Venous Bolus Injection
Nugroho, Akhmad Kharis; Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta Indonesia
Hakim, Lukman; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, INDONESIA
Monolix, NONMEM, WinBUGS-PKBUGS, sparse data, therapeutic drug monitoring
Monolix, NONMEM, and WinBUGS-PKBUGS are among available software package for population-based modeling. The sparse condition of drug plasma concentration versus time (Cp-time) data is prevalent in clinically based studies involving patients. It is not ethical in this case, to collect a many and large volumes of blood samples. This study was aimed to simulate the capability of Monolix, NONMEM, and WinBUGS-PKBUGS to analyze very sparse Cp-time data after an intravenous bolus drug administration and to estimate the minimum number of Cp-time data required for an adequate analysis. Data of Cp-time were obtained based on simulation using the pharmacokinetic one-compartment open model following an intravenous bolus administration of 50 mg of a hypothetical drug. In this respect, six random values of k (rate constant of elimination) and Vd (volume of distribution) with mean and standard deviation values of 0.3 ±0.1 per hour and 30 ± 10 L, respectively, were used to create simulated Cp-time data of 6 subjects. Simulated Cp-time data in each subject were randomly ranked to choose data based on the intended number of samples in each subject. Several sparse Cp-time data scenarios, starting from a very limited state, i.e., with a total of 6 Cp-time data (1 datum per subject) to a rich situation with 48 Cp data (8 data per subject), were examined.The goodness of fit evaluations, as well as the similarity of individual values of k and Vd to the respective real values (p>0.05), indicate that nonlinear-mixed-effect-model using Monolix, NONMEM and WinBUGS-PKBUGS can appropriately describe sparse Cp-time data even with only 2 data per subject. This fact is an important finding to support the demand of analytical tool for a limited number of Cp-time data such as obtained in therapeutic drug monitoring event.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Hibah Penelitian Dasar Unggulan Perguruan Tinggi, a research grant scheme provided by the Ministry of Research, Technology and Higher Education, the Republic of Indonesia, year 2018.
2019-10-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1403
10.14499/indonesianjpharm30iss4pp293
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 293
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1403/923
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/440
2020-02-19T02:37:07Z
3:ART
PROTOPLAST SUSPENSION CULTURE OF LEAVES MESOPHYLOF Centella asiatica (L.) Urban AND QUALITATIVE ANALYSIS ASIATICOSIDE
Prihastanti, Erma; Faculty of Science and Mathematic, Diponegoro University
Semarang
Soegihardjo, C. J.; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
Purbaningsih, Susiani; Faculty of Biology
Universitas Gadjah Mada
Yogyakarta
Centella asiatica leaves has been used as diuretic, antihypertensi, anti leprae, skin infection, burning skin and celloid. Cells suspenson culture of mesophyll of Centella asiatica leaves were carried out in three steps: isolation, purification, and cell culture. The aim of this research is to investigate the influences of leaves position (age of leaves) and concentration of macerozyme R-10 for cells number and viability, and the influence of sucrose concentration for cells growth and biosynthesis of asiaticoside. Isolation and purification of mesophyll cell has been performed using factorial completely randomized design. The first factor was leaf position (age of leaves), and the second factor was concentration of macerozyme. Collected data were analysed using Anova and Duncan’s test at 0.0 – 2.5 – 5.0 and 7.5 per cent respectively. Asiaticoside produced by cell biomass were analyzed qualitatively using thin layer chro- matography. The result revealed that mesophyll cells could be used as explant for suspension culture. The highest biomass produced with highest viability were found in cells isolated from second leaves treated with 0.1% macerozyme (1.32 . 107 cells/ml). Addition of sucrose to suspension culture medium produced higher packed cells volume (pcv) percentage than no sucrose was added, (0% sucrose gave pcv 8.5%, 2.5% sucrose pcv 22%, sucrose 5% pcv 21.5%, and 7.5% sucrose pcv 15.75%). The asiaticoside production not only depended on sucrose, because without any sucrose was added, the asiaticoside was also available..Key Words: Cell suspension culture, mesophyll, Centella asiatica (L.) Urban, asiaticoside
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/440
10.14499/indonesianjpharm0iss0pp10-19
Indonesian Journal of Pharmacy; Vol 12 No 1, 2001; 10-19
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/440/319
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1184
2018-10-23T02:19:20Z
3:ART
The Effect of Dual Antiplatelet Post Percutaneous Coronary Intervention On Aggregation of Platelet In Myocardial Infarction Patients With Diabetes Mellitus and Non Diabetes Mellitus
Rahmawati, Yessi Asli; Airlangga University
Yogiarto, Mohammad; Dr.Soetomo Teaching Hospital Surabaya
Zulkarnaen, Bambang Subakti; Airlangga University
To analyze the differences in the effect of dual antiplatelet post PCI on the percentage of aggregation in myocardial infarction patients with DM and non DM. Percentage of aggregation were analyzed using light transmission aggregometry (LTA) before loading dose, after PCI, and after maintenance dose of dual antiplatelet (aspirin 100mg and clopidogrel 75mg). Total 22 patients were participated in this study divided into 10 and 12 patients in diabetic and non diabetic group. Percentage of aggregation after taking dual antiplatelet maintenace dose decrease significantly in both group (p=0.006 in diabetic group and p=0.002 in non diabetic group). Mean reduction of percentage of aggregation in diabetic group (3.30±2.91%) is less than non diabetic group (6.83±5.97%). Statistical analysis shows that the mean reduction of percentage of aggregation between two groups were not significantly different (p>0.05). Mean percentage of aggregation after dual antiplatelet maintenance dose was higher in diabetic group and mean reduction of percentage of aggregation was higher in non diabetic group, although statistically in both group it is not significantly different.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-01-17
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1184
10.14499/indonesianjpharm29iss1pp44
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 44
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1184/862
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1740
2021-01-06T13:13:53Z
3:ART
Cytotoxic Potential of Arthrospira platensis Extract on Cervical Cancer Cells Line Hela: Study on Antiproliferative, Cell Cycle, Apoptosis Induction and Anti Metastasis
Nurani, Laela Hayu; Faculty of Pharmacy, Universitas Ahmad Dahlan
Mahfudh, Nurkhasanah; Faculty of Pharmacy, Universitas Ahmad Dahlan
Gandjar, Ibnu Gholib
Rahayu, Intan; Faculty of Pharmacy, Universitas Ahmad Dahlan
A. platensis; cervical cancer; HeLa cells; HFDa cells
Cervical cancer can be treated conventionally with chemotherapy agents, but its use has side effects and complications in the form of damage to normal cells. This study aims to determine the potential of A. platensis as an alternative anticancer agent that is selective towards normal cells. Based on TLC analysis, A. platensis contains antioxidant compounds such as β-carotene, flavonoids, and terpenoids which are able to inhibit proliferation and trigger apoptosis of cancer cells. The study was conducted using cervical cancer cells HeLa and normal cells HDFa. A. platensis macerated with 96% ethanol at a ratio of 1:4. Based on probit analysis, it is known that ethanol extract of A. platensis has a cytotoxic effect on HeLa cells with IC50 values of 260.444μg/mL and index selectivity towards HDFa cells of 7.931. The mechanism of cytotoxic activity of ethanol extract of A. platensis is related to its ability to extend the doubling time, increase the induction of apoptosis, and reduce the rate of cells migration. Ethanol extract of A. platensis can also increase cells accumulation in the S phase to prevent cells from entering the G2/M phase.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-04-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1740
10.14499/indonesianjpharm31iss1pp19
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 19
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1740/939
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1740/281
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/513
2018-09-05T01:49:55Z
3:ART
SYNTHESIS AND ANTIMICROBIAL ACTIVITY EVALUATION OF SOME SCHIFF BASES DERIVED FROM 2-AMINOTHIAZOLE DERIVATIVES
Gupta, Rajul; Dept. of Pharmacy, CMJ University, Modrina Mansion, Laitumkhrah, Shillong, Meghalaya-793003, India.
Fuloria, Neeraj Kumar; Dept. of Pharmaceutical Chemistry, Anuradha College of Pharmacy, Chikhli, Dist. Buldana, Maharashtra, 443201, India.
Fuloria, Shivkanya; Dept. of Pharmaceutical Chemistry, Anuradha College of Pharmacy, Chikhli, Dist. Buldana, Maharashtra, 443201, India.
Various substituted acetophenones (1-5) on treatment with iodine and thiourea yielded 2-amino-4-(substituted-phenyl)thiazole (1a-5a), which on further treatment with various substituted aldehydes to get N-(substitutedbenzylidene)-4(substitutedphenyl)thiazol-2-amine (1b-5b). All the synthesized compounds were characterized by their respective FTIR, H NMR and Mass data. Synthesized compounds (1b-5b) were subjected to investigation for their antibacterial and antifungal studies against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Asperigillus flavus and Asperigillus fumigatus by disk diffusion method. Compound 5b was found to be most effective with largest zone of inhibition. Key words: Thiazole, Acetophenones Antibacterial, Antifungal, Substituted Aldehydes.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-09-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
text/html
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/513
10.14499/indonesianjpharm24iss1pp35-39
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 35-39
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/513/391
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/513/912
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1285
2018-10-23T02:19:33Z
3:ART
Physical Characterization and Dissolution Study of Pentagamavunon-0 Loaded Self Nano-Emulsifying Drug Delivery System
Astuti, Ika Yuni; Universitas Muhammadiyah Purwokerto
Marchaban, Marchaban; Universitas Gadjah Mada
Martien, Ronny; Universitas Gadjah Mada
Nugroho, Agung Endro; Universitas Gadjah Mada
PGV-0 SNEDDS; characterization; dissolution
The recent work focuses on the physical characterization and dissolution study of PGV-0 loaded self-nanoemulsifying drug delivery system (SNEDDS). The PGV-0 SNEDDS was prepared by spontaneous emulsification method using oleic acid, tween 20:labrasol (1:1) and polyethylene glycol 400. The zeta potential of the PGV-0 SNEDDS was -34.4 ± 7.66 mv. The nanoemulsion of PGV-0 SNEDDS was thermodynamically stable with forming droplet oil containing PGV-0 in it and spherical. The dissolution result showed that the PGV-0 SNEDDS was able to increase the dissolution significantly (p <0.05) compared with PGV-0 powder of 44.13% in AGF medium and 30.37% in AIF medium. It is concluded that SNEDDS PGV-0 was able to increase the PGV-0 aqueous solubility.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-06-22
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1285
10.14499/indonesianjpharm29iss2pp60
Indonesian Journal of Pharmacy; Vol 29 No 2, 2018; 60
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1285/871
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1221
2019-10-10T01:30:23Z
3:ART
Effect of Atorvastatin Treatment on Vascular Aterogenic Factors (Lipid Profiles and VCAM-1) in Patient Diabetes with Dyslipidemia
Mukti, Asri Wido; Airlangga University
Atorvastatin, Adhesion molecules, Diabetes mellitus, , dyslipidemia, VCAM-1.
To analyze effectiveness of atorvastatin 20mg on lipid profiles and adhesion molecule VCAM-1 in patient with diabetes dyslipidemia. An observational prospective cohort study was conducted from November 2016 to March 2017. Patients who fulfilled the inclusion criteria were taken twice for their lipid profiles and VCAM-1 measurements (before initiation of study and after 6 weeks treatment of atorvastatin 20mg). There were 13 patients who met the inclusion criteria. The results of 13 patients showed that after 6 weeks of atorvastatin therapy, there was a 28% decrease in total cholesterol (t0=223.77±49.69, t1=160.92±24.69), 39% LDL decrease (t0=152.59±44.25, t1 =93±21.44), a decrease in TG 38.6% (t0=200.85±101.53, t1=123.30±62.77) and a statistically significant decrease in VCAM-1 7.47% (t0=729.59±208.06, t1=675.06±182.88). The results of the correlation test between total cholesterol and VCAM-1 (p=0.185, r=0.268), LDL and VCAM-1 (p=0.127, r=0.307), TG and VCAM-1 (p=0.198, r=0.261) showed no correlation. Based on the results of the study, it can be concluded that atorvastatin therapy can provide improvements in atherogenic factors such as decreased lipid profile and VCAM-1, and there was no correlation between lipid profile and VCAM-1 in type 2 DM patients with dyslipidemia.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-04-11
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1221
10.14499/indonesianjpharm30iss2pp128-132
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 128-132
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1221/899
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1813
2020-02-19T02:45:03Z
3:ART
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/673
2020-01-27T02:51:49Z
3:ART
PASSIVE AND IONTOPHORETIC PERMEATION OF CAPTOPRIL GEL: AN IN VITRO AND IN VIVO STUDY
Jain, Ashish; Bansal College of Pharmacy, Kokta, Anand Nagar, Bhopal-462021, India.
Nayak, Satish; Bansal College of Pharmacy, Kokta, Anand Nagar, Bhopal-462021, India.
Soni, Vandana; Department of Pharmaceutical Sciences, Dr H.S.Gour Vishwavidyalaya, Sagar 470003, M.P. India
The Objective of this work was to formulate and evaluate captopril gel to assess its suitability for transdermal delivery by passive and iontophoresis. A polymer gel was prepared using hydroxypropyl methyl cellulose and in vitro skin permeability was assessed in full thickness skin of rabbits and pigs. For in vivo studies New Zealand rabbits were used. In vitro passive permeation was carried out in Franz diffusion cell but for iontophoresis, diffusion cell was modified according to Glikfield design. Iontophoresis was performed at a current density of 0.5 mA/cm2via silver /silver chloride electrodes with passive controls but for in vivo study current density wasreduced to 0.1 mA/cm2. Blood samples were analyzed for drug content by HPLC. Results of the in vitro study indicated that iontophoresis considerably increased the permeation rate of captopril compared to passive controls in both the skin types (P<0.01). The plasma concentration of captopril was significantly higher (P<0.001) than that obtained in the passive controls. Results showed that the target permeation rates for captopril could be achieved with the aid of iontophoresis by increasing the area in an appreciable range.Key words: Captopril, iontophoresis, transdermal, Rabbit, Pigskin, in vitro, in vivo.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/673
10.14499/indonesianjpharm23iss2pp121-129
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 121-129
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/673/539
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1487
2019-10-28T01:20:02Z
3:ART
Phytochemical analysis, antioxidant, antibacterial and antidiarrheal activities of Limnophila repens
Akter, Afroza; Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
Basher, Mohammad Anwarul; Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
Roy, Roni; Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
Sultana, Sharmin; Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
Daula, A F M Shahid Ud; Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
Limnophila repens; phytochemical content; antioxidant; antibacterial; antidiarrheal.
The study was carried out to determine the phytochemical content, antioxidant, antibacterial and antidiarrheal activity of methanolic extract of whole plant of Limnophila repens. The total phenolic, flavonoids, flavonols, tannins, β-carotene, lycopene, chlorophyll-α, and chlorophyll-β contents were found to be 65.21 ± 0.004 mg GAE/g , 44.83 ± 0.003 mg QE/g , 17.21 ± 0.002 mg QE/g, 56.27 ± 0.002 mg GAE/g , 1.32 ± 0.01 µg/g, 0.93 ± 0.012 µg/g, 11.85 ± 0.04 mg/g and 9.69 ± 0.037 mg/g, respectively. In vitro antioxidant potential of L. repens was assessed using various methods like by 2,2-diphenyl-1 picrylhydrazyl (DPPH), hydroxyl radical scavenging assay, reducing power assay, FRAP assay and thiobarbituric acid assay. IC50 values of the aforesaid tests were found to be 2.33 mg/ml, 442.31 µg/ml, 374 µg/ml, 55.09 µg/ml, and 4.30 mg/ml, respectively. The total antioxidant capacity was observed 140.7 ± 0.004 mg/g AAE. Aantibacterial activity of the methanolic extracts of whole plant of L. repens was evaluated using the disk diffusion method. The plant extract didn’t show any activity against Gram-positive (except Staphylococcus aureus) and Gram-negative bacteria. Finally, castor oil induced method was conducted to investigate the antidiarrheal activity of L. repens. At the dose of 400 mg/kg, the plant extract (35.62% inhibition of diarrhea) exhibited stronger effect compared to standard drug, loperamide (27.4% inhibition of diarrhea).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-09-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1487
10.14499/indonesianjpharm30iss3pp187
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 187
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1487/917
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/658
2020-01-27T02:53:58Z
3:ART
HEPATOPROTECTIVE EFFECT OF GAMAVUTON-0 AGAINST D˗GALACTOSAMINE/LIPOPOLYSACCHARIDE-INDUCED FULMINANT HEPATIC FAILURE
Nurrochmad, Arief; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta 55281,
Indonesia
Sari, Ika Puspita; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta 55281,
Indonesia
Murwanti, Retno; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta 55281,
Indonesia
., Sardjiman; Curcumin Research Center, Faculty of Pharmacy, Universitas Gadjah Mada
Yogyakarta 55281, Indonesia.
Candraningrum, Triana; Curcumin Research Center, Faculty of Pharmacy, Universitas Gadjah Mada
Yogyakarta 55281, Indonesia.
Afritasari, Dyah; Curcumin Research Center, Faculty of Pharmacy, Universitas Gadjah Mada
Yogyakarta 55281, Indonesia.
Martina, Devina; Curcumin Research Center, Faculty of Pharmacy, Universitas Gadjah Mada
Yogyakarta 55281, Indonesia.
Siahaan, Iren Wati; Curcumin Research Center, Faculty of Pharmacy, Universitas Gadjah Mada
Yogyakarta 55281, Indonesia.
The objective of this study is to determine the hepatoprotective effect of GVT-0 (one of curcumin analogues) against liver damage in rat-induced D-galactosamine (D-GalN)/lipopolysaccharide (LPS) as a model of fulminant hepatitis. In the study D˗GalN/LPS elevated serum GPT activity that indicate a particular occurrence of liver damage due to depletion of UTP and UDP-glucuronic acid. Administration of GVT-0 (10 mg/kg) showed decreased enzyme activity of SGPT/SGOT but had no effect on serum ALP and total bilirubin levels, whereas at doses of 20 and 40 mg/kg, the protective effect of GVT-0 was decrease. The glutathione content in the D-GalN/LPS (0.76 ± 0.07) mol/g liver content was found lower than controls (0.90 ± 0.03) mol/g liver. Administration of GVT-0 dose of 10, 20 and 40 mg/kg restored glutathione content returned to normal levels. The results showed that treatment of GVT-0 showed no effect on TBARS and catalase activity. Treatment of D-GalN/LPS, indicating the trend of increased TNF-α, although statistically not significant, while the administration of GVT-0 showed a tendency to decrease the concentration of TNF-α. All findings of the results indicated that the GVT-0 mainly lower dose (10 mg/kg) showed hepatoprotective action in rat model of fulminant hepatitis induced by D-GalN/LPS. The results indicated that the mechanism of hepatoprotective effect of GVT-0 is not via antioxidant properties of GVT-0. However, further studies are necessary to explain the molecular mechanism of hepatoprotective effect of GVT-0.Key words: Gamavuton-0, hepatoprotective, fulminan hepatitis, D˗galactosamine/LPS
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/658
10.14499/indonesianjpharm23iss1pp18-26
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 18-26
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/658/524
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/690
2020-01-27T02:53:31Z
3:ART
DEVELOPMENT THE VALIDATION OF INDONESIAN VERSION OF SF-36 QUESTIONNAIRE IN CANCER DISEASE
Perwitasari, Dyah Ariani; Faculty of Pharmacy, Ahmad Dahlan University
Jl. Kapas No.9, Semaki, Yogyakarta, Indonesia
55166.
A Cancer patients need long term therapy in the treatment of their disease. One of the outcome of cancer treatment in patients of quality of life. Because of the limited instrument for measuring cancer patients’ quality of life in Indonesian version, thus this study is aimed to develop the validation of Indonesian version of Short Form-36 questionnaire in cancer patients. The observational study was carried out in this study. Data were collected from cancer patients in the Oncology Department of Sardjito Hospital, Yogyakarta, Indonesia, who were treated with cisplatin at the dosage of ≥ 50mg/m2 as monotherapy or in combinations. The validation process involved known-group validity, discriminant validity, convergent validity and factor analysis. About 203 subjects who were diagnosed with cancer were recruited in this study. Theknown-group validity test showed that there were no significant differences of SF-36 domains among different diagnosa. All of the questions met the criteria of convergent validity except for the questions number 26, 28, 30 and 32. Furthermore, the questions number 2 and 28 did not meet the criteria of discriminant validity. This study presents that many questions are needed to be reconstructed, due to the result of factor analysis which showed that those questions are loaded significantly with other domain’s questions. The development of Indonesian version of SF-36 scales in cancer disease is still less unstatisfactory. According to the result of this study, future study with new construction of questions in this version is still necessary.Key words: validation, SF-36, Indonesia, cancer
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/690
10.14499/indonesianjpharm23iss4pp248-253
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 248-253
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/690/556
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1378
2019-10-03T03:16:50Z
3:ART
Synthesis and Molecular Docking Studies of N’-benzoylsalicylhydrazide derivatives as antituberculosis through InHA enzym inhibition
Santosa, Harry; University of Surabaya
Putra, Galih Satrio; University of Surabaya
Yuniarta, Tegar Achsendo; University of Surabaya
Budiati, Tutuk; Airlangga University
Antituberculosis, Benzoylsalicylhydrazide, Methyl Salicylate, InHA, Microwave synthesis, Molecular docking
The specific aims of this study is to synthesize and to study the possible mechanism of N’-benzoylsalicylhydrazide derivatives as an antituberculosis agent through InhA (Enoyl acyl carrier protein reductase) inhibition using in silico method. Five analogues of N’-benzoylsalicylhydrazide were synthesized using microwave irradiation from methyl salicylate as starting material, which yielded 80-90% product on average. This indicates a considerable improvement in terms of effectivity and efficiency, compared to the more conventional method using reflux condition. Character-ization of the compounds were subsequently carried out by UV, FTIR, 1H-NMR, 13C-NMR spectroscopy, which confirmed that the compounds had been successfully synthesized. Ultimately, molecular docking was performed using Molegro Virtual Docker (MVD) on the active site of InhA enzyme to predict the activity of the compounds. The results showed that all compounds performed comparatively well against N-(4-Methylbenzoyl)-4-benzylpiperidine as the native ligand and also yielded lower docking score than isoniazide (INH). From this study it can be concluded that N’-benzoylsalicylhydrazide derivatives could be synthesized using microwave irradiation with good product yield and all of the synthesized analogues are suggested to possess antituberculosis activity via InhA enzyme inhibition. In vitro activity will have to be determined in the future to validate whether N’-benzoylsalicylhydrazide derivatives perform well as a potential antituberculosis agent.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-11-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1378
10.14499/indonesianjpharm29iss4pp198
Indonesian Journal of Pharmacy; Vol 29 No 4, 2018; 198
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1378/884
Copyright (c) 2018 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1259
2018-10-23T02:18:57Z
3:ART
Development And Validation Of Stability Indicating RP-HPLC Method For Determination Of Ceritinib
Adhao, Vaibhav Suresh; Dr. Rajendra Gode College of Pharmacy, Malkapur.
Sharma, J; Dr. B.R. Ambedkar University, Uttar Pradesh, India
Thakre, M; Dr. B.R. Ambedkar University, Uttar Pradesh, India
Stability-indicating, HPLC, Ceritinib, Validation, Stress Testing
A new, simple, specific, accurate and precise RP-HPLC method was developed for determination of Ceritinib. In the present study, stress testing of Ceritinib was carried out according to ICH guidelines Q1A (R2). Ceritinib was subjected to stress conditions of hydrolysis, oxidation, photolysis and neutral decomposition. Extensive degradation was found to occur in acidic, condition. Mild degradation was observed in basic and at thermal conditions. Successful separation of drug from degradation products formed under stress conditions was achieved on a Hypersil BDS C18 column (250 mm × 4.6 mm, 5.0 μ particle size) using acetonitrile: acetate buffer (pH 3.7 ± 0.05) (50:50 v/v), at a flow rate of 1.0 mL/min and column was maintained at 40˚C. Quantification and linearity was achieved at 272 nm over the concentration range of 5 - 100 μg/mL for Ceritinib. The method was validated for specificity, linearity, accuracy, precision, LOD, LOQ and robustness.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-01-09
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1259
10.14499/indonesianjpharm28iss4pp241
Indonesian Journal of Pharmacy; Vol 28 No 4, 2017; 241
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1259/857
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1506
2019-10-10T01:29:33Z
3:ART
Inhibitory Activity of Sargassum hystrix Extract and Its Methanolic Fractions on Inhibiting α-Glucosidase Activity
Azizi, Wirdatul Auliya; Department of Fisheries Faculty of Agriculture Universitas Gadjah Mada Jalan Flora Gedung Perikanan A4 Bulaksumur Yogyakarta 55281
Ekantari, Nurfitri; Department of Fisheries Faculty of Agriculture Universitas Gadjah Mada Jalan Flora Gedung Perikanan A4 Bulaksumur Yogyakarta 55281
Husni, Amir; Department of Fisheries Faculty of Agriculture Universitas Gadjah Mada Jalan Flora Gedung Perikanan A4 Bulaksumur Yogyakarta 55281
α-Glucosidase, Fraction, Methanol, Sargassum hystrix
Seaweed has a great potential in the pharmaceutical field, one of them as antidiabetic. The purposed of this study was to know the inhibitory activity of Sargassum hystrix extract and its methanol fraction in inhibiting α-glucosidase activity. S. hystrix was extracted using methanol, then partitioned using chloroform, ethyl acetate, and methanol. Methanol fraction then separated by column chromatography to obtain the compound. The crude extract, the partitioned methanol fraction, and the column chromatography fraction were tested for its activity on inhibiting the α-glucosidase. The compounds of active fraction were analyzed using gas chromatography-mass spectrometry (GC-MS). The inhibitory activity (IC50) of the crude extracts and the partitioned methanol fraction were 0.35±0.05 and 0.02±0.00 (mg/mL), respectively. The column chromategraphy fractions that had an inhibitory activity to α-glucosidase were M2 (23.46±1.63%), M3 (30.88±4.53%), M4 (73.64±3.47%), and M7 (53.48±1.56%). GC-MS showed that the suspected compound which had inhibiting α-glucosidase in methanol fraction were 9-Octadecenoic acid, 1-Heptadecanecarboxylic acid,9,12-Octadecadienoic acid (Z, Z), and Octadecanoic acid methyl ester.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-03-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1506
10.14499/indonesianjpharm30iss1pp36
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 36
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1506/891
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1986
2021-01-07T01:00:39Z
3:ART
Design and Synthesis of Novel Derivatives of 4-(6-(4-Substituted Phenyl)-7H-[1,2,4] Triazolo[3,4-b][1,3,4]Thiadiazin-3- yl) Phenol as a Potent Inhibitor of Tubulin with Antitumor Activity
Kubba, Ammar A. Razzak Mahmood; Department of Pharmaceutical Chemistry, College of Pharmacy-University of Baghdad, Bab-Al-Mouadam-10001, Baghdad
Shihab, Wurood Ahmed; Department of Pharmaceutical Chemistry-College of Pharmacy/University of Ashur, Al-waziriya, Baghdad/Iraq
Al-Shawi, Nada Naji; Department of Pharmacology and Toxicology, College of Pharmacy-University of Baghdad, Bab-Al-Mouadam-10001, Baghdad/Iraq
Jabir, Majid Shamki; Division of Biotechnology/ Department of Applied Science/University of Technology, Baghdad/Iraq
Synthesis; novel series; anti-proliferative; tubulin-targeting.
A new series of 4-(6-(4-substituted phenyl)-7H[1,2,4] triazolo[3,4b][1,3,4]thiadiazin-3-yl) phenol was synthesized and explored for anti-tubulin activity using in silico and in vitro assay models. The starting nucleus, ethyl-4-hydroxybenzoate was treated with hydrazine hydrated, and converted to 4-hydroxybenzohydrazide (1). Then, (1) was stirred with CS2 and KOH in dry EtOH to afford 2-(4-. hydroxyl benzoyl)hydrazine-1-carbodithioate, as a potassium salt (2). Compound (2) was used directly and refluxed with hydrazine hydrate to yield a parent nucleus, 4-(4-amino-5-mercapto-4H-1,2,4-triazol-3-yl)phenol (3). The target compounds (4-7), were synthesized by refluxing compound (3) with various substituted phenacyl bromides using sodium acetate as a abase, The new compounds (3-7) were characterized using different spectroscopic analysis techniques including IR, and 1HNMR. The anticipated modes of binding to α- tubulin were also investigated by means of a molecular docking experiment. The binding mode revealed good agreement with the in vitro work with binding energies of (-38.77 and -37.50 kcal/ mol) for the most potent compounds 7 and 4, respectively. The effect of synthetic novel compounds on cancer cell lines proliferation was screened by MTT assay. The ability of the synthetic compounds to induce apoptosis process was tested using acridine orange/ ethidium bromide staining. The synthetic novel compounds exhibited potent inhibitory effects on the growth and viability of cancer cell lines SKOV-3 and AMJ-13 cells. They inhibited the proliferation and growth of cancer cell lines at low concentrations, with IC50 values ranging from10.44 to 19.67 µg/mL against SKOV-3 cells, and for AMJ-13 cells, the IC50 values were11.35 to 20.52 µg/mL. The effect of the synthetic compounds on the cell growth, and proliferation of cancer cell lines was associated with increased apoptosis.Our results demonstrated that the target compounds inhibited cancer cell lines proliferation, with a mechanism of action parallel to that of other tubulin inhibitors
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
University of Baghdad-College of Pharmacy
2020-08-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1986
10.14499/indonesianjpharm31iss2pp92
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 92
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1986/955
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/668
2020-01-27T02:51:49Z
3:ART
IN VIVO EVALUATION OF MISOPROSTOL FLOATING MICROSPHERES
Vanitha, Kondi; Vishnu institute of pharmaceutical
education and research, Medak, A.P., India
Varma, Mohan; Shri Vishnu college of pharmacy,
Bhimavaram, A.P.,India
Ramesh, Allure; Vishnu institute of pharmaceutical
education and research, Medak, A.P., India
Prostaglandin (PG) has been reported to be an important protective and acid suppressive factor in the gastric mucosa. The objective of the study was to develop and evaluate a stomach specific drug delivery system for controlled release of Misoprostal a PE analogue for gastric ulcer induced NSAIDs. Floating microspheres were prepared by emulsification-solvent evaporation method using ethyl cellulose as a polymer, carbopol as mucoadhesive polymer. Ulcers were induced by the oral administration of absolute ethanol (5 mL/kg) to 24 h fasted Wistar male rats (n=8), weighing 200 g. Sodium bicarbonate solution, misoprostal solution and drug loaded microspheres were tested. Formulations were administered orally 1h before the administration of ethanol. Prior to the oral administration, rats were anesthetized with ethylic ether. After 2 h of ethanol administration, animals were sacrificed; the stomachs were removed, opened along he greater curvature and examined for lesion measurements. Ulcer indexes (UI) were calculated. The in vivo evaluation showed that ulcer index values were 0.61 ± 0.14 for the sodium bicarbonate solution, 0.58 ±0.18 for the misoprostal and 0.11 ± 0.06 for the misoprostal-loaded microspheres. The KruskalWallis test detected statistical differences (p =0.002) between the ulcer indexes. The multiple analyses (Student-Newman-Keuls) showed that the misoprostal loaded microspheres presented a gastric ulcer index statistically lower than that of the sodium bicarbonate solution (p = 0.001) and the misoprostal solution (p = 0.021). The percentages of ulceration inhibition were 31 and 75% after the administration of misoprostal solution and microspheres, respectively. In conclusion, the in vivo evaluation showed that the microspheres presented ulcer index lower than the solutions, showing that misoprostal-loaded microspheres were efficient in protecting the stomach against ulcer formation.Key words: Misoprostal, microspheres, ulcer index,
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/668
10.14499/indonesianjpharm23iss2pp99-103
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 99-103
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/668/534
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1339
2018-11-02T06:46:53Z
3:ART
Physical and Chemical Properties of Native and Fully Pregelatinized Cassava Starch (Manihot esculenta Crantz)
Dewantara Putra, I Gusti Ngurah Agung; Udayana University
Murwanti, Retno; Universitas Gadjah Mada
Rohman, Abdul; Universitas Gadjah Mada
Sulaiman, T.N Saifullah; Universitas Gadjah Mada
cassava starch fully pregelatinized, native cassava starch, physical properties, chemical properties, amylose, amylopectin
Starch is widely used as an excipient in pharmaceutical formulations because it is inert and it can be mixed with drugs without any chemical reactions. This study was aimed to develop and to characterize the physical and chemical properties of cassava starch fully pregelatinized (CSFP) and native cassava starch (Manihot esculenta Crantz) (NCS). Organoleptic properties, pH, ash content, shrink drying, macroscopic and microscopic analyses, amylose and amylopectin content, bulk and tapped density, the angle of repose and flow rate were physically evaluated for both type of cassava starch. Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), energy-dispersive x-ray spectroscopy (EDS), and differential scanning calorimetry (DSC) were used to characterize and evaluate the chemical properties of the CSFP and NCS. The results of this study indicate that CSFP exhibited different values of those determined parameters compared to that of NCS organoleptic properties i.e, pH, viscosity, ash content, shrink drying, macroscopic and microscopic analyses, amylose and amylopectin content, bulk and tapped density, angle of repose and flow rate. The measurement results with DSC obtained Tg at NCS of 68.18oC while in CSFP there is no Tg because cassava starch (CS) is fully gelatinized. In conclusion, CSFP as a good profile starch contained a higher amount of amylose with larger particle size and good particle density and viscosity than the natural starch and improve its flow properties and compactibility. CSFP had a noticeable effect on fragility, hardness, disintegration time and percentage of drug release from the tablets produced, that can be developed as a pharmaceutical excipient in development of solid dosage forms (sustain release).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
LPDP BUDI
UPT Forensik Udayana University
2018-09-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1339
10.14499/indonesianjpharm29iss3pp145
Indonesian Journal of Pharmacy; Vol 29 No 3, 2018; 145
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1339/876
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1435
2019-10-28T01:20:02Z
3:ART
Metabolite Profiling of Eleutherine palmifolia (L.) Merr. By HPTLC-Densitometry and its Correlation with Anticancer Activities and In Vitro Toxicity
Mutiah, Roihatul; Department of Pharmacy, Faculty of Medicine and Health Science, Maulana Malik Ibrahim Islamic State University of Malang
Hadya, Chorida Muhjatul; Department of Pharmacy, Faculty of Medicine and Health Science, Maulana Malik Ibrahim Islamic State University of Malang
Ma'arif Z.A, Burhan; Department of Pharmacy, Faculty of Medicine and Health Science, Maulana Malik Ibrahim Islamic State University of Malang
Bhagawan, Weka Sidha; Department of Pharmacy, Faculty of Medicine and Health Science, Maulana Malik Ibrahim Islamic State University of Malang
Annisa, Rahmi; Department of Pharmacy, Faculty of Medicine and Health Science, Maulana Malik Ibrahim Islamic State University of Malang
Indrawijaya, Yen Yen Ari; Department of Pharmacy, Faculty of Medicine and Health Science, Maulana Malik Ibrahim Islamic State University of Malang
Huwaida, Fadhila Isma; Department of Pharmacy, Faculty of Medicine and Health Science, Maulana Malik Ibrahim Islamic State University of Malang
Ramadhani D.A., Ria; Department of Pharmacy, Faculty of Medicine and Health Science, Maulana Malik Ibrahim Islamic State University of Malang
Susilowati, Retno; Department of Biology, Faculty of Science and Technology, Maulana Malik Ibrahim Islamic State University of Malang
Taufik, Imam; National Agency of Drug and Food Control, Ambon
Eleutherine palmifolia (L.) Merr., Metabolite profile, the profile of anticancer activity, profile toxicity
Eleutherine palmifolia (L.) Merr. (E. palmifolia) is a plant that used as raw material for herbal medicines. The difference of growing location the medicinal plants affects the difference of metabolite content, It also affects the pharmacological activity and toxicity of the plant. This study aims to determine the profile of metabolites (metabolite fingerprinting), anticancer profiles, toxicity/safety profiles from several different regions in Indonesia for the purpose of authentication, efficacy, safety and quality control of these plants. Samples were sampled from 6 different locations in Indonesia, they were West Java, Central Java, East Java, East Borneo, Central Borneo, and South Borneo. Metabolite fingerprinting was determined by HPTLC-densitometry method, the profile of anticancer activity and toxicity was known by the MTT-ELISA method. The difference between metabolite fingerprinting, anticancer profile, toxicity/safety profile was analyzed by Principal Component Analysis (PCA) and Hierarchical Component Analysis (HCA). Whereas the relation between metabolite fingerprinting, anticancer profile, toxicity/safety profile was analyzed by Partial Least Square (PLS). The results of the metabolite profile analysis with PCA showed that E. palmifolia from 6 different locations gave different metabolite profiles and there were 3 metabolites that had a significant effect on cluster formation. samples are Rf 055, 059, and 044. The results of the HCA analysis showed that the 6 regions are in one cluster the same one. The analysis of PLS-DA showed that the metabolites for anticancer activity are found in Rf 059, 076, 093 and Rf 034. While the metabolites for toxicity are Rf 002, Rf 044 and Rf 059.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Ministry of Religion Research Grant funds of the International Applied Global Research
2019-07-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1435
10.14499/indonesianjpharm30iss3pp157
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 157
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1435/908
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/685
2020-01-27T02:53:31Z
3:ART
ENHANCEMENT OF DISSOLUTION RATE OF MODAFINIL USING SOLID DISPERSIONS WITH POLYETHYLENEGLYCOLS
Jigar, Vyas; Department of Pharmacy, Bhagwant University, Sikar Road, Ajmer, Rajasthan, India. 305004
Jayvadan, Patel; Nootan Pharmacy College, Visnagar, Gujarat, India. 384315
A., Jain D.; Department of Pharmacy, Bhagwant University, Sikar Road, Ajmer, Rajasthan, India. 305004
Solid dispersions (SDs) of modafinil (MDF) were prepared using polyethyleneglycols (PEGs), in 1;1, 1;2 and 1;4 proportions by fusion, solvent evaporation and physical mixing method. Differential scanning calorimetry (DSC) and X-ray powder diffractometry (XRD) were used to examine the physical state of the drug. The data from the XRD showed that the drug was converted to amorphous form as the number and intensity of peaks were decreased in solid dispersion as compared to pure drug and physical mixture of drug and carrier. DSC thermograms also confirmed the change in physical state of the drug as the peaks were altered or disappeared. With the highest ratio of the carriers (1:4), the drug solubility was enhanced by 38.68, 34.78 and 9.29 folds in solvent evaporation, fusion and physical mixing methods respectively. Solid dispersion batch S6 containing drug:PEG6000 in 1:4, was selected to be formulated as tablet (batch TS6) and evaluated for in vitro drug dissolution & six month stability. An increased dissolution rate of modafinil was observed from SDs and PMs, as compared to pure crystalline drug. The dissolution rate of modafinil from its PMs or SDs increased with an increasing amount of polymer.Key words: Fusion, solvent evaporation, physical mixture, in vitro dissolution, characterization.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/685
10.14499/indonesianjpharm23iss4pp209-215
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 209-215
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/685/551
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1646
2021-01-06T13:13:53Z
3:ART
Antidiabetic Activity of Okra Fruit (Abelmoschus esculentus (L) Moench) Extract and Fractions in Two Conditions of Diabetic Rats
Herowati, Rina; Faculty of Pharmacy
Setia Budi University
Indonesia
Abelmoschus esculentus (L) Moench, STZ-NA induced diabetes, insulin resistance diabetes, pancreatic cell regeneration, glucose transporter-4.
Okra (Abelmoschus esculentus (L) Moench) fruit is empirically used in type 2 diabetes mellitus treatment. This research aims to know the antihyperglycemic activity of okra fruit extract and fractions in streptozotocin-nicotinamide (STZ-NA) induced as well as in insulin resistance diabetic rats, the effect on pancreatic cells regeneration, and the effect on immunohistochemical expression of glucose transporter-4. This study used a group of 35 male Wistar rats for STZ-NA induced diabetic model and another group of 35 rats for insulin resistance diabetic model. Gliclazide (0.72mg/kg BW) and metformin (45mg/kg BW) were used as drug control in STZ-NA induced and insulin resistance diabetes, respectively. Okra fruit ethanol extract, n-hexane, ethyl acetate, and water fraction were orally administered with dose of 200; 107; 6 and 86mg/kg BW, respectively, for 28 days after diabetic condition was obtained. Blood glucose level was measured every week. Hematoxylin-eosin staining was used to evaluate the pancreatic cells regeneration, while immunohistochemistry was used to evaluate the expression of glucose transporter-4 in muscle membrane cells, at the end of the treatment. The results revealed that ethyl acetate fraction was the most effective in lowering blood glucose level in both condition of diabetes. Ethyl acetate fraction decreased the necrosis of pancreatic cells in STZ-NA induced diabetic rats and increased the expression of glucose transporter-4 in muscle cell of insulin resistance diabetic rats.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-04-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1646
10.14499/indonesianjpharm31iss1pp27
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 27
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1646/940
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/508
2020-02-19T02:37:07Z
3:ART
AKTIVITAS PENGANGKAPAN RADIKAL POLIFENOL DALAM DAUN TEH
Rohdiana, Dadan; Tea and Quinine Research Center
Gambung PO BOX 1013 Bandung 40010
Ability of free radical scavengers of various tea extracts (black tea, fermented tea; green tea , unfermented tea; oolong dan pouchong tea semifermented tea) was studied. The scavenging effects on active oxygen decreased in order semifemented tea > unferfmnted tea > fennented lea. Antioxidant activity on tea often might related wthi catechin presence. Catechin activity as antioxidant estabilished. Several epidemilogical and phartnacokgical research shown that catechin had a strongly antioxidan activity. (-)-epigallocatechin gallate (EGCG) and (-)-epicatechin gallate (ECG) are the major catechin of ~ tea This compounds had a strongly scavenging activity on free radical such as superoxide, hydrogen superoxide, DPPH and peroxynitdte. This review aim to exploited~ tea Oyphenols ability as free radicals sw vengers Key word: antioxidant activity, active oxygen, scavenging effect
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/508
10.14499/indonesianjpharm0iss0pp52-58
Indonesian Journal of Pharmacy; Vol 12 No 1, 2001; 52-58
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/508/386
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1226
2018-10-23T02:18:57Z
3:ART
Provision of Patient Medication Counseling Service In A Tertiary-Care Eye Hospital In Bandung: A Gap Analysis of Patient’s Need, Standard and Service Provider Resources
Hendrayana, Tomi; Department of Pharmacology-Clinical Pharmacy, School of Pharmacy, Bandung Institute of Technology
Zazuli, Zulfan; Department of Pharmacology-Clinical Pharmacy, School of Pharmacy, Bandung Institute of Technology
Fadhila, Genialita; Department of Pharmacology-Clinical Pharmacy, School of Pharmacy, Bandung Institute of Technology
Cahyono, Nur Sidik; Department of Pharmacology-Clinical Pharmacy, School of Pharmacy, Bandung Institute of Technology
Provision of new service; patient medication counseling service; gap analysis; tertiary-care eye hospital; pharmaceutical care
Many studies showed that counseling can improve patient adherence and reduce medication-related problems leading to reach an optimal outcome therapy. The aims of this study were to identify patient’s need, healthcare professional’s support, pharmacist’s readiness and step to be taken to meet standard for implementing patient medication counseling service. Questionnaires has been given to hospitalized-patient to identify the patient’s need and to healthcare professionals in recognizing their supports, followed by survey of pharmacist’s readiness. Gap analysis was then carried out to identify the additional resources to be provided for conducting the service. About 103 patients were enrolled and eager for drug counseling to be implemented with pharmacist as healthcare counselor. Approximately 46 nurses and 25 active-medical-doctors were participated and giving their support for pharmacist to provide the service; and pharmacists are ready to conduct patient medication counseling with minor adjustment. Gap analysis concluded that additional resources should be furnished, e.g. information module consist of standard information needed by the patient, standard operational procedure, and updated literatures. Due to lack of pharmacist, counseling service only be delivered to patient who has a complex antibiotic regimen therapy. Our data showed that patient medication counseling service in this center could be quickly implemented.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-12-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1226
10.14499/indonesianjpharm28iss4pp232
Indonesian Journal of Pharmacy; Vol 28 No 4, 2017; 232
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1226/856
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1446
2019-10-10T01:29:33Z
3:ART
The Force Degradation Study of The Morpholinium 2-((4-(2-Methoxyphenyl)-5-(Pyridin-4-YL)-4H-1,2,4-Triazol-3-YL) Thio) Acetate
Varynskyi, Borys; Zaporozhye State Medical University
Kaplaushenko, Andriy; Zaporozhye State Medical University
1,2,4-triazoles, LC MS, forced degradation
The aim of the current research was to make force degradation study of the morpholinium 2-((4-(2-methoxyphenyl)-5-(pyridin-4-yl)-4H-1,2,4-triazol-3-yl)thio)acetate (API) for additional confirmation of the selectivity of API and impurity determination method, propose possible structures of degradation products. Agilent 1260 Infinity (degasser, binary pump, autosampler, column thermostat, diode array detector) was used. Mass selective detector was single-quadrupole LC/MS 6120 with electrospray ion source (ESI). The chromatography study was carried out by isocratic elution with a water-acetonitrile mixture (84:16) with the addition of 0.1% methanoic acid. A column Zorbax SB-C18, 30 mm x 4.6 mm, 1.8 um. A column temperature was 40oC. The flow rate was 0.400 ml/min. The period of exposition of stress factors was four days. The period of exposition of stress factors was four days. Influence of the such factors as acid, alkali, H2O2, temperature, UV radiation on the API in bulk drug, 0.1% solution and 1% “solution for injection” were studied. Dependence of content of API from a number of days of factors exposition was studied. Most destructive action was during the influence of the H2O2 and UV radiation. API was stable during the storage at laboratory conditions, acid, alkali and temperature (60oC) influence. Some degradation products structure was proposed.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Zaporizhzhia State Medical University
2019-03-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1446
10.14499/indonesianjpharm30iss1pp25
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 25
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1446/890
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1654
2021-01-07T01:00:39Z
3:ART
Application of Simplex Lattice Design on the Optimization of Andrographolide Self Nanoemulsifying Drug Delivery System (SNEDDS)
Indrati, Oktavia; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia;
Doctoral Programme in Pharmaceutical Science, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia
Martien, Ronny; Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada
Sekip Utara, Yogyakarta Indonesia
Rohman, Abdul; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada Sekip Utara, Yogyakarta Indonesia
Nugroho, Akhmad Kharis; Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada
Sekip Utara, Yogyakarta Indonesia
andrographolide; snedds; simplex lattice design
Background: Optimization of self-nanoemulsifying drug delivery system (SNEDDS) formulation is an important step to obtain optimal formulation with desired characteristics.Objective: This present study was aimed to utilize simplex lattice design in optimizing andrographolide SNEDDS.Method: Simplex lattice design was employed to optimize andrographolide SNEDDS in which component of SNEDDS was selected as the independent factor while the charactheristics of SNEDDS was used as the responses. Capryol-90, Kolliphor RH 40, and propylene glycol were selected as the oil, surfactant, and co-surfactant, respectively. Optimization of andrographolide SNEDDS formulation was based on their characteristics including emulsification time, droplet size, and drug content. The optimized SNEDDS formulation was evaluated for emulsification time, droplet size, drug content, and zeta potensial.Results: The emulsification time, droplet size, drug content, and zeta potensial of the optimized andrographolide SNEDDS was found to be 1.21±0.03 min, 44.02±0.67 nm, 6.69±0.08 mg/g, and -40.63±0.76 mV, respectively.Conclusion: This result suggested that simplex lattice design is a suitable for efficiently optimizing the formulation of andrographolide SNEDDS.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
the Indonesia Endowment Fund for Education (BUDI DN-LPDP)
2020-07-10
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1654
10.14499/indonesianjpharm31iss2pp124
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 124
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1654/952
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/663
2020-01-27T02:53:58Z
3:ART
CHROMOSOME CHARACTERIZATION OF THREE VARIETIES OF GINGER (Zingiber officinaleRosc.)
Daryono, Budi Setiadi; Genetics Laboratory, Faculty of Biology
Universitas Gadjah Mada Jl. Teknika Selatan-Sekip Utara Yogyakarta 55281
Rahma, Siti Nur Azizah Fauziati; Genetics Laboratory, Faculty of Biology
Universitas Gadjah Mada Jl. Teknika Selatan-Sekip Utara Yogyakarta 55281
., Purnomo; Laboratory of Plant Systematics, Faculty of
Biology, Universitas Gadjah Mada. Jl. Teknika
Selatan-Sekip Utara Yogyakarta 55281
., Sudarsono; Departement of Pharmaceutical Biology,
Faculty of Pharmacy, Universitas Gadjah Mada,
Sekip, Utara Yogyakarta 55281, Indonesia
Giant ginger (Zingiber officinale Rosc. var. officinale), red ginger (Zingiber officinale Rosc. var. rubra) and small ginger (Zingiber officinale Rosc. var. amarum) are three varieties of Zingiber officinale Rosc. They have a lot of benefit and often used by Indonesian as a traditional drug. Moreover, they have a big chance to be use as a flavor in world wide. Therefore, research for their quality, quantity and continuity of supplyare needed. Characterization of their chromosomes is one effort for improving ginger cultivation. The objective of this research was to study mitotic time and chromosome characters of three varieties of ginger. Squashing method was used for chromosome preparation. The results showed that mitotic time of giant ginger is 09.00-10.05 am, red ginger is 09.00-10.30 am, while small ginger is 08.45-11.00 am. Chromosome number of giant ginger and small ginger are 2n=2x=30, while red ginger is 2n=2x=22. Giant ginger has R= 3,109, Red ginger has R = 3,206 and small ginger has R = 4,065. Based on chromosome characters it is revealed that relationship between giant ginger and red ginger is closer that of compare to small ginger. This result is important as basic information for improving the gingers production through breeding program.Key words: Zingiber officinale Rosc., mitotic time, chromosome characterization, squashmethod
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/663
10.14499/indonesianjpharm23iss1pp54-59
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 54-59
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/663/529
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1330
2018-10-23T02:19:33Z
3:ART
Formulation of Ketorolac Tromethamine for Controlled Release in Gastrointestinal and Colonic Delivery System
Hossain, Md. Amzad; Department of Pharmacy, University Science and Technology Chittagong, Bangladesh.
Shill, Swapon Kumar; Professor of Chemistry, University of Chittagong, Bangladesh
Khan, Shakhawat Hasan; Professor of Chemistry, University of Chittagong, Bangladesh
Hossen, S. M. Moazzem; Department of Pharmacy, University of Chittagong, Chittagong-4331, Bangladesh.
Uddin, Mohammad Nasir; Professor of Chemistry, University of Chittagong, Bangladesh
Drug Formulation, Ketorolactromethamine, gastrointestinal and colonic delivery system, HPLC analysis
A suitable matrix system of ketorolac tromethamine (KTR) formulation has been developed with the aim of increasing the contact time, achieving controlled release, reducing the frequency of administration, improving patient compliance. In this concern an enteric-coated KTR matrix tablet intended for specific delivery of drugs to the colon by combining the use of a time dependent core with a pH-sensitive film coating. Eudragit L100, with a threshold pH 7, was selected as coating material. New formulation is proved to be noble as to KTR delivery through both gastrointestinal and colonic system. New formulation is considered to reduce gastrointestinal side effects and achieve high local drug concentration at the afflicted site in the gastro-intestine and colon.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-07-09
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1330
10.14499/indonesianjpharm29iss2pp49
Indonesian Journal of Pharmacy; Vol 29 No 2, 2018; 49
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1330/864
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1550
2019-10-10T01:30:23Z
3:ART
Hepatoprotective effects of Curcumin-Mesoporous Silica Nanoparticles on CCl 4 -induced Hepatotoxicity Wistar rats
Hadisoewignyo, Lannie; Faculty of Pharmacy, Widya Mandala Catholic University Surabaya, Jalan Raya Kalisari Selatan No. 1, Pakuwon City, Surabaya, Indonesia, 60112
Soeliono, Ivonne; Faculty of Pharmacy, Widya Mandala Catholic University Surabaya, Jalan Raya Kalisari Selatan No. 1, Pakuwon City, Surabaya, Indonesia, 60112
Hartono, Sandy Budi; Faculty of Engineering, Widya Mandala Catholic University Surabaya, Jalan Kalijudan No. 37, Surabaya, Indonesia, 60114
Hestianah, Eka Pramhyrta; Faculty of Veterinary Medicine, Airlangga University, Campus C, Mulyorejo, Surabaya, 60115
Mahanani, Sri Rahayu; Faculty of Pharmacy, Widya Mandala Catholic University Surabaya, Jalan Raya Kalisari Selatan No. 1, Pakuwon City, Surabaya, Indonesia, 60112
curcumin, curcumin nanoparticles, hepatoprotection, carbon tetrachloride.
It has been reported that curcumin has a hepatoprotective effect, but its low solubility limited its utilization. Recently there was so many emerging research of advanced curcumin formulation, such as nanoparticles curcumin. In our previous study, curcumin has been loaded into mesoporous silica nanoparticles (C-MSN). This study was performed both to evaluate of C-MSN hepatoprotective effect in CCl4-induced rats. Sixteen rats were divided into four groups, namely normal and CCl4 control, curcumin, C-MSN group. Treatment was given according to its group for fourteen days consecutively. At day 14, three hours after the last administration, CCl4 (1,25 ml/kgBB) were administered orally. Twelve hours later the rats were sacrificed, and blood samples were drawn from their hearts. Blood serum examination result revealed that C-MSN caused a significantly lower ALT and AST than CCl4 control group (851±271 U/L vs 1734±275 U/L; 295±155 U/L vs 1348±235 U/L; p<0.05). Its effect on hepatic serum level resembled curcumin group. However, the result was not supported by histology examination which showed a higher number of necrotic hepatic cells in C-MSN group than in the curcumin group (147±9 vs 80±16; p<0.05). From this study, it can be concluded that C-MSN revealed an excellent hepatoprotective property, but it was suspected that MSN itself has the toxic effect on the liver. A further study of MSN toxicity was needed to support its safety use.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-05-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1550
10.14499/indonesianjpharm30iss2pp114-121
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 114-121
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1550/904
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/680
2020-01-27T02:53:16Z
3:ART
EXPRESSION OF RECOMBINANT HUMAN ERYTHROPOIETIN WITH GLYCOSYLATION MODIFICATION IN HEK293T CELLS
Septisetyani, Endah Puji; Research Center for Biotechnology, Indonesian Institute of Sciences Jl. Raya Bogor KM 46,
Cibinong, Bogor, 16911, Indonesia
Rubiyana, Yana; Research Center for Biotechnology, Indonesian Institute of Sciences Jl. Raya Bogor KM 46,
Cibinong, Bogor, 16911, Indonesia
Wisnuwardhani, Popi Hadi; Research Center for Biotechnology, Indonesian Institute of Sciences Jl. Raya Bogor KM 46,
Cibinong, Bogor, 16911, Indonesia
Wardiana, Andri; Research Center for Biotechnology, Indonesian Institute of Sciences Jl. Raya Bogor KM 46,
Cibinong, Bogor, 16911, Indonesia
Santoso, Adi; Research Center for Biotechnology, Indonesian Institute of Sciences Jl. Raya Bogor KM 46,
Cibinong, Bogor, 16911, Indonesia
Stability of erythropoietin (EPO) depends on its glycosylation states. With more glycosylation sites, the EPO protein will be more stable and also increase its half-life. A construct of recombinant human erythropoietin (rhEPO) which contains 2 additional N-link for glycosylation were designed. Based on translation analysis using ORF (open reading frame)-finder and protein alignment analysis using blast-p of NCBI home page, expected recombinant hEPO with additional 6-histidin tag in carboxyl terminus was expressed. HEK293T cells were transfected with recombinant plasmid containing rhEPO by using calcium phosphate method. Expression of rhEPO was detected by dot blot and Western blot analysis using hEPO antibody as the primary antibody and antirabbit antibody with alkaline phospatase linked as the secondary antibody. The bands were detected by BCIP/NBT color development substrate. The data indicated detection of EPO in culture medium of transfected HEK293T cells.Key words: HEK293T cell, calcium phosphate transfection, N-linked glycosylation, recombinant human erythropoietin
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/680
10.14499/indonesianjpharm23iss3pp177-182
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 177-182
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/680/546
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1567
2022-10-26T01:05:53Z
3:ART
The Effect of Ursolic Acid from Plantago Lanceolata Leaves on Leukocytes Migration and Chemokines Level
Fakhrudin, Nanang; Faculty of Pharmacy, Universitas Gadjah Mada
Center for Natural Antiinfective Research, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
Franyoto, Yuvianti Dwi; Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia.
Sekolah Tinggi Ilmu Farmasi “Yayasan Pharmasi Semarang,” Semarang 50193, Indonesia.
Astuti, Eny Dwi; Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia.
Nurrochmad, Arief; Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia.
Wahyuono, Subagus; Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia.
Center for Natural Antiinfective Research, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281, Indonesia
plantago, anti-inflammatory agents, leukocyte, chemokine CCL2, interleukin-8
Initially considered as a normal body response to injury, inflammation is currently known as a major event contributing to the development of many human disorders. Many drugs and bioactive molecules have been discovered from medicinal plants and the number is still growing by time. Among those medicinal plants used in folk medicines, Plantago lanceolata is used to cure inflammatory-related diseases. In our previous study, we showed that the n-hexane insoluble fraction of P. lanceolata leaves (HIF) demonstrated a potent anti-inflammatory activity by inhibiting leukocytes migration in mice. This study aimed to identify the anti-inflammatory compound from the HIF and to investigate the effect on the chemokines level. P. lanceolata leaves were initially macerated with dichloromethane. The dried extract was partitioned using n-hexane to obtain n-hexane soluble fraction (HSF) and n-hexane insoluble fraction (HIF). Both fractions were evaluated for their anti-inflammatory activities in thioglycollate-induced leukocyte migration. The active fraction (HIF) was subjected to preparative thin-layer chromatography (TLC) to isolate the major compound. The structure of the compound was identified based on NMR, IR, and Mass spectra. Moreover, we investigated the effect of the compound on the level of chemokines responsible for leukocytes migration. The active compound was identified as ursolic acid, based on its spectral data. Ursolic acid at the dose of 30, 60, and 120mg/kg BW inhibited leukocyte migration and reduced chemokines level (IL-8 and MCP-1).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Directorate General of Higher Education, Ministry of Education and Culture, Republic of Indonesia
2019-11-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1567
10.14499/indonesianjpharm30iss4pp252
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 252
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1567/924
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/441
2020-02-19T02:37:07Z
3:ART
PREFORMULATION OF DICLOFENAC SODIUM SUSTAINED RELEASE BY ION EXCHANGER RESINE
Zulkarnain, A. Karim; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
Yuwono, Tedjo; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
., Sumarno; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
The aim of the study was to find out a formula of granules giving a constant release of drug and also to search for the influence of pH on the drug release from the formula. One type of drug-resin complexes was used in this study ion exchanger the complex of diclofenac sodium and dowex. After 12 hours mixing the drug-resin complex was dried at 40 oC in an oven for 3 days. Afterwards, they were granulated and shieved. The particle size used were 20-40 mesh. The granules were tested for the dissolution of diclofenac sodium from the granules. The dissolution experiments were performed in a modified model of USP XX and the dissolution media used were phosphate buffer with the pHs of 5,8; 6,8 and 7.6 and the temperature was maintained at 37 oC. The amount of diug released in to the medium was assayed spectrophotometrically. The extent of dissolution was expressed as dissolution efficiency in 8 hours (DE8, % ). The results showed that the extent of dissolution of dtclofenac sodium from the granules complexed with the resin were low. The correlation between the amount of drug release from the complex and time was linier with the highest coefficient correlation having the ratio of l:5. The higher the pH values of the media, the higher the drug release from granules was. The drug release from drug-resin complex was well controlled which may be used for making a sustained release dosage form.Key Word : sustained release, diclofenac sodium, ion exchanger resin, granules
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/441
10.14499/indonesianjpharm0iss0pp20-27
Indonesian Journal of Pharmacy; Vol 12 No 1, 2001; 20-27
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/441/320
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1767
2021-01-06T13:13:53Z
3:ART
Synthesis and Preliminary In Vitro Anti-inflammatory Evaluation of Mannich Bases Derivatives of 4’-Methoxy-substituted of Asymmetrical Cyclovalone Analogs
Rahmawati, Nur
Hariyanti, Hariyanti
Saputri, Fadlina Chany
Hayun, Hayun; Faculty of Pharmacy
Universitas Indonesia
Depok
West Java
Mannich bases derivatives, Asymmetrical, Cyclovalone, synthesis, in-vitro anti-inflammatory, protein denaturation.
Two of Mannich bases derivatives of 4’-methoxy-substituted of asymmetrical cyclovalone analog (ACA) (2a and 2b) were synthesized. The synthesized compounds and the other two Mannich bases derivatives of 4'-methoxy-substituted ACA (2c and 2d) were evaluated for their in-vitro anti-inflammatory activity preliminary by protein denaturation inhibition method using a final concentration of 1.57 μM. The study found that all the Mannich bases exhibited anti-inflammatory potential with inhibition ranging from 33.17- 42.47%. The activity of 2b (42,47%) and 2d (41.90%) was higher than that of diclofenac sodium (35.27%) and the parent compound 1 (38.16%). As a conclusion, 2b and 2d have a prospect as a potential candidate for an anti-inflammatory agent. Further study should be done using more specific methods.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Directorate of Research and Community Services, Universitas Indonesia, Depok, Indonesia, for the financial support (PITTA Grant, 2018)
Laboratory of Organic Chemistry, Institut Teknologi Bandung for recording NMR and mass spectra.
2020-04-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1767
10.14499/indonesianjpharm31iss1pp35
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 35
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1767/941
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/514
2018-09-05T01:49:55Z
3:ART
EFFECTS OF AVURVEDIC SHODHANA (PROCESSING) ON DRIED TUBEROUS ACONITE (Aconitum napellus Linn.) ROOT
Paul, Arindam; Department of Pharmacology and Pharmacognosy, Babaria Institute of Pharmacy, BITS edu campus, Vadodara-Mumbai NH#8, Varnama, Vadodara - 391240, Gujarat, INDIA
Gajjar, Umang; Department of Pharmacology and Pharmacognosy, Babaria Institute of Pharmacy, BITS edu campus, Vadodara-Mumbai NH#8, Varnama, Vadodara - 391240, Gujarat, INDIA
Donga, Jignasa; Department of Pharmacology and Pharmacognosy, Babaria Institute of Pharmacy, BITS edu campus, Vadodara-Mumbai NH#8, Varnama, Vadodara - 391240, Gujarat, INDIA
Aconite (Aconitum napellus Linn.) commonly known as atis is a poisonous plant used extensively as antihypertensive, antipyretic, analgesic and antirheumatic. Ayurveda recommended the administration of aconite roots only after purification, i.e., boiling roots in cow’s urine (Gomutra). In the present study an attempt was made to compare the pro-arrhythmic and antihypertensive effects of powdered aconite root purified by shodhana process with that of unpurified form of aconite roots in order to provide scientific support of the claim in ayurvedic texts that purification of aconite root by shodhana process retains its antihypertensive activity and is devoid of pro-arrhythmic activity. Aconite root treatment in both forms purified and unpurified) caused significant reduction in BP when compared with diseased control group (P<0.05). The unpurified aconite root group showed significant increase in heart rate, increase in QRS complex time and increase in QT interval, however these parameters were statistically insignificant in purified aconite root treated group. The PRA, SC and BUN levels was significantly decreased in aconite root treatment groups. The probable mechanism of antihypertensive activity of aconite root can be attributed to decrease in plasma renin activity, decrease in oxidative stress and increase in NO levels. Key words: Aconite, shodhana process, antihypertensive, proarrhythmic
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2013-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/514
10.14499/indonesianjpharm24iss1pp40-46
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 40-46
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/514/392
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1284
2018-10-23T02:19:20Z
3:ART
Hypoglycemic Activity and Pancreas Protection of Combination Juice of Mengkudu (Morinda citrifolia Linn.) Juice and Temulawak (Curcuma xanthorrhiza Roxb.) Juice on Streptozotocin-Induced Diabetic Rats
Santoso, Bilal Subchan Agus; Academy of Pharmacy of Putra Indonesia Malang
Sudarsono, Sudarsono; Universitas Gadjah Mada
Nugroho, Agung Endro; Universitas Gadjah Mada
Murti, Yosi Bayu; Universitas Gadjah Mada
hypoglycemic, pancreas protection, combination juice, mengkudu, temulawak
Mengkudu fruit contains scopoletin and temulawak rhizome contains curcumin have been observed because they have strong antioxidant activity and they were used traditionally as antidiabetic. This research aimed to evaluate effect of antihyperglycemic and pancreas protection of juice combination of mengkudu fruit juice (MFJ) and temulawak rhizome juice (TRJ) on diabetes rats which were inducted with streptozotocin (STZ). Rats were grouped into 7 groups, each of group consisting 5 rats. Each of group was treated accordingly for 28 days except for a normal group of rats. Rats taken blood from the plexus retroorbitalis for examination of blood glucose levels every week. On the last day, rats blood was examined for malondialdehyde (MDA) levels. After that the rats were turned off for examination of pancreatic morphological conditions. The results showed that diabetic rats given MFJ-TRJ combination juice experienced a significant decrease in blood glucose levels, a significant decrease in MDA levels and improvement pancreas morphology when compared with the negative control group. The conclusion of this research was giving combination juice of MFJ-TRJ juice can decreased blood glucose level, decreased MDA level and can improved pancreas morphology condition.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Faculty of Pharmacy of Universitas Gadjah Mada
2018-04-17
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1284
10.14499/indonesianjpharm29iss1pp16
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 16
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1284/848
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1478
2019-10-10T01:30:23Z
3:ART
Synthesis and in Silico Studies of a Benzenesulfonyl Curcumin Analogue as a New Anti Dengue Virus Type 2 (DEN2) NS2B/NS3
Ikhtiarudin, Ihsan; Department of Pharmacy, Sekolah Tinggi Ilmu Farmasi Riau, Jalan Kamboja, Pekanbaru, 28293, Indonesia
Curcumin, Dengue virus, Molecular docking, Molecular dynamic.
Curcumin has been reported can interact with multiple molecular targets involved in a large variety of diseases. Accumulated evidence indicated curcumin plays an inhibitory role against infection of numerous viruses. Some studies have been reported that curcumin can interfere the infection processes of dengue virus. In this work, a benzenesulfonyl curcumin, (3E,5E)-3,5-bis(4-methoxybenzylidene)-1-(phenylsulfonyl)piperidin-4-one (compound 2) has been synthesized by two steps of reactions. The structure of compound 2 has been established based on the interpretation of spectral data include UV, FT-IR, MS/MS, 1H and 13C NMR. Then, the in silico studies have been also performed to predict the potency of compound 2 as inhibitor for dengue virus Type 2 (DEN 2) NS2B/NS3 protease. The in silico studies showed that compound 2 has hydrogen bonding with His51 residue, and amazingly that the other catalytic triad such as Asp75 and Ser135 were also showed interactions with the ligand. It is presumably that this compound showed very good activity against DEN2 and can be developed as a new inhibitor for dengue viruses.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-04-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1478
10.14499/indonesianjpharm30iss2pp84-90
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 84-90
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1478/898
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1832
2020-01-23T01:19:36Z
3:ART
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/674
2020-01-27T02:51:49Z
3:ART
IDENTIFICATION OF SAFETY ALERT BY MONITORING ANALYTICAL PARAMETERS AND HIGH-RISK DRUGS
Vilaplana, Vicente Escudero-; Pharmacy Department, Gregorio Marañón
University General Hospital Doctor Esquerdo 46, 28007 Madrid, Spain
Antúnez, María Gómez-; Internal Medicine Department, Gregorio
Marañón University General Hospital, Madrid, Spain
García, Esther Durán-; Pharmacy Department, Gregorio Marañón
University General Hospital Doctor Esquerdo 46, 28007 Madrid, Spain
Míguez, Antonio Muiño-; Internal Medicine Department, Gregorio
Marañón University General Hospital, Madrid, Spain
Sáez, María Sanjurjo-; Pharmacy Department, Gregorio Marañón
University General Hospital Doctor Esquerdo 46, 28007 Madrid, Spain
Early detection of adverse drug reactions (ADR) increases patient safety. Our objective was to identify ADR by monitoring laboratory parameters and high-risk drugs. We carried out a two-month prospective observational study in a Internal Medicine Department, with daily recording of drugs prescribed and the following parameters: Na, K, Ca, serum creatinine, glomerular filtration rate (GFR), INR, glucose, haemoglobin, platelets, ALT, AST, bilirubin, GGT, alkaline phosphatase, TSH, T4, and blood digoxin. High-risk drugs were closely monitored. 52 patients included, of whom 46.2% experienced an ADR. We observed an association with drugs in 25.5%, as follows: reduction in GFR, 26.9% (associated with loop diuretics [41.7%], angiotensin-converting enzyme [ACE] inhibitors [33.3%], angiotensin II receptor blockers [ARB] [16.6%], andanti-diabetic drugs [8.3%]); hypokalemia, 22.3% (associated with loop diuretics [50.0%], potassium-free fluid [37.5%], and salbutamol [12.5%]); hyperkalemia, 14.4% (associated with ACE inhibitors [60.0%] and ARB [40.0%]); INR out of range, 10.8% (associated with drug interactions [66.7%]); hyperglycemia, 8.1% (associated with corticosteroids [66.7%] and anti-diabetic drugs [33.3%]); and other conditions, 18.8%. We conclued that patient safety could be improved by implementing warnings in electronic prescriptions in cases of a decrease in GFR or modification of potassium levels in patients who are prescribed loop diuretics, ACE inhibitors, or ARBs.Key words: Adverse drug reaction, clinical decision support, high-risk drug, safety
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/674
10.14499/indonesianjpharm23iss2pp130-134
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 130-134
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/674/540
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1527
2019-10-28T01:20:02Z
3:ART
Effect of Soybean-based Food Supplement on Insulin and Glucose Levels in Type 2 Diabetes Mellitus Patients
Purwoko, Akhmad Edy; Department of Pharmacology and Toxicology, Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Yogyakarta, Indonesia
Astuti, Indwiani; Department of Pharmacology and Therapy, Faculty of Medicine - Public Health and Nursing, Universitas Gadjah Mada Yogyakarta, Indonesia
Asdie, Ahmad Husain; Department of Internal Medicine, Dr. Sardjito Central General Hospital Yogyakarta, Indonesia
Sugiyanto, Sugiyanto; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
soybean supplements, insulin, blood glucose, type 2 diabetes mellitus, GLP-1 gene.
Introduction: Indonesia is one of ten countries with the largest diabetic populations in the world. Giving soy supplements to people with diabetes can reduce blood glucose levels significantly. However, no studies have shown a reduction back to normal glucose levels. This study aimed to determine the increase of insulin levels and blood glucose suppression in glucose tolerance tests after supplementation of soy in patients with type 2 diabetes. Sequencing the gene of GLP-1 (37 amino acid). This experimental research was a randomized, treatment controlled; open clinical trial study conducted by comparing the control group treated with soybean supplement products containing 18g protein and placebo. Seventy-six treatment subjects with type 2 diabetes were recruited from Muhammadiyah Gamping Hospital and PERSADIA gymnastics group according to the inclusion and exclusion criteria and then grouped randomly in the supplement treatment and placebo groups. Blood sampling was drawn at 8 hours fasting, 2 hours after supplement administration and 2 hours after administration of 75g glucose for measuring blood insulin and glucose level. Blood insulin level 2 hours after supplement administration (mean±SEM) increased 5.3±0.8µIU/ml (n=37) while placebo decreased 0.9±0.4µIU/ml (n=39) which was statistically significantly different (p<0.05). Blood glucose levels increased in the glucose tolerance tests, while the supplement group was 130.0±11.5mg/dl (n=37) lower than the placebo 146.7±8.2mg/dl (n=39) (p>0.05). Gene sequencing shows a nucleotide variation of GLP-1 (37 amino acid) in Javanese T2DM Giving Soybean supplements containing 18g protein increased blood insulin levels and suppressed blood glucose levels in glucose tolerance tests.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-09-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1527
10.14499/indonesianjpharm30iss3pp208
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 208
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1527/918
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/657
2020-01-27T02:53:58Z
3:ART
EFFECT OF BENZALDEHYDE EXCESS IN THE SYNTHESIS OF LR-2 AND CYTOTOXIC ACTIVITY OF LR-2 AGAINTS HeLa CELL
Ritmaleni, Ritmaleni; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip, Utara Yogyakarta 55281, Indonesia
Arifin, Muhammad Fajar; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip, Utara Yogyakarta 55281, Indonesia
Laksmiani, Ni Putu Linda; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Udayana, Bali 80221, Indonesia.
., Rumiyati; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip, Utara Yogyakarta 55281, Indonesia
., Sismindari; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip, Utara Yogyakarta 55281, Indonesia
LR-2(4-phenyl-3,4-dihydro-indeno[2’,1’]pyramidine-2(1H)- thione; Leni Ritmaleni 2), which designed and assumed to have biologically activity as anticancer, has been successfully synthesized by using the Biginelli reaction. This research was aimed to investigate the effect of benzaldehyde excess in the synthesis of LR-2 and to evaluate the cytotoxic activity of LR-2against HeLa cancer cell lines. The synthesis was done by reacting benzaldehyde, 2-indanone and together with thiourea at one time as said as one pot reaction synthetic methodology and the reaction was acid catalysed. The mole equivalent of benzaldehyde was in excess compare to others. The effect of benzaldehyde in excess is the higher the mole of benzaldehyde, the lower the yield of LR-2. The cytotoxicity of LR-2 was done by using MTT method and the LC50 was 268.15 μM.Key words : LR-2, benzaldehyde, cytotoxic, HeLa
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/657
10.14499/indonesianjpharm23iss1pp9-17
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 9-17
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/657/523
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
c96878201c8ed19161f3909e46438be4