2024-03-28T18:12:46Z
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/oai
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1355
2018-11-02T06:46:53Z
3:ART
The Effect of Ethanol Extract of Piper nigrum L. Fruit on Reproductive System in Adult Male Wistar Rats: A Study of FSH, LH, Testosterone Level and Spermatogenic Cells
Ekaputri, Tia Wida; Master Programme, Basic Medical and Biomedical Science, Faculty of Medicine, Universitas Gadjah Mada, Indonesia
Sari, Ika Puspita; Department of Pharmacology & Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada
Rizal, Dicky Moch.; Department of Physiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Indonesia
black pepper (Piper nigrum L.), FSH, LH, male rat, testosterone, spermatogenic cells
Exploration to find out new natural contraceptive agent for male is still being developed. Black pepper (Piper nigrum L.) and its main alkaloid piperine have potential antifertility because of sitotoxic and hormonal effects. The aim of this study is to find out the effect of ethanolic extract of black pepper (Piper nigrum L.) fruit of on reproductive hormone serum level, sperm quality, and spermatogenic cell populations in adult male Wistar rat. Twenty five male rats were divided into five groups consisting of two control group, i.e. K(-) (Na-CMC 0.5%), K(+) (finasteride 0.45mg/kg BW), and three groups received different doses of black pepper fruit ethanolic extract, i.e. D(1) (3.33mg/kg BW), D(2) (6.66mg/kg BW) and D(3) (13.32mg/kg BW) respectively. The treatment were given to each group for 55 days. Reproductive parameters were measured, including serum level of reproductive hormones (FSH, LH, dan testosteron), quality of cauda epididymal sperm (spermatozoa concentration, motility and morphological abnormality), spermatogenic cell populations (primary spermato-cyte and spermatid count) and seminiferous tubules diameter. Ethanol extract of black pepper fruit at doses of 3.33mg/kg BW, 6.66mg/kg BW, and 13.32mg/kg BW increased serum FSH level. Extract at dose of 13.32mg/kg BW decreased serum LH level, while extract at doses of 6.66mg/kg BW and 13.32mg/kg BW decreased serum testosterone level. The number of primary spermatocytes, spermatozoa concentration, and spermatozoa motility were decreased by administration of ethanol extract of black pepper fruit with dose of 6.66mg/kg BW and 13.32mg/kg BW. Ethanol extract of black pepper fruit at dose of 6.66mg/kg BW and 13.32mg/kg BW had a negative impact on the male reproductive system and showing potential antifertility in male rat.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-09-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1355
10.14499/indonesianjpharm29iss3pp136
Indonesian Journal of Pharmacy; Vol 29 No 3, 2018; 136
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1355/875
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1442
2019-10-10T01:29:33Z
3:ART
Determination of Classification Model and Total Phenolic Content of Local Variety Soursop (Annona muricata L.) Leaf Powder in Different Altitudes Using NIR and FTIR Spectroscopy with Chemometrics
Wulandari, Lestyo; Department of Pharmaceutical chemistry, Faculty of Pharmacy University of Jember, 68121, Jember, Indonesia
Nugraha, Ari Satia; Department of Pharmaceutical chemistry, Faculty of Pharmacy University of Jember, 68121, Jember, Indonesia
Siswanti, Yashinta Nirmala; Division of Clinical and Community Pharmacy, Faculty of Pharmacy, The University of Jember, 68121, Jember, Indonesia
Annona muricata L., total phenolic Content, NIR, FTIR, chemometric
Soursop (Annona muricata L.) leaves have been widely used traditionally to overcome health problems, this is related to its total phenolic content. This study was aimed to determine the classification model and total phenolic content of soursop leaf powder of local varieties, in different altitudes using NIR and FTIR spectroscopy with chemometrics. Local variety soursop leaf samples that have been collected from low land (0-200 meter above sea level (masl)), medium land (201-700 masl) and high land (>700 masl) are prepared, then scanned using NIR and FTIR spectroscopy. Furthermore, the NIR spectra data from the samples were used as predictors on the LDA classification model of local and queen varieties soursop leaves to identify sample varieties. Samples identified as local varieties, whose total phenolic content was determined using a comparative method (UV-Vis spectrophotometry). The highest mean of total phenolic content is owned by samples from medium land (Jember) of 5.72% w/w GAE, followed by low land (Bangkalan) 2.95% w/w GAE and high land (Batu) 1, 78% w/w GAE. NIR and FTIR spectra data belonging to the samples were analyzed by chemometrics qualitatively using LDA, SVM and SIMCA, and quantitatively using PLS, PCR and SVR. The best classification and calibration model are formed from the NIR spectra data, that are the LDA model with an accuracy of 100% and the PLS model with an R-square calibration value of 0.998071 and RMSEC of 1.2735631. The LDA and PLS models are applied to the real samples. The results of the sample's total phenolic content determination obtained from the NIR spectroscopy method and UV-Vis spectrophotometry method were then tested with Paired-Samples T Test and it can be concluded that the content obtained from the two methods did not have a significant difference.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1442
10.14499/indonesianjpharm30iss1pp7
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 7
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1442/888
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1250
2019-04-29T06:33:14Z
3:ART
The employment of UV-spectroscopy combined with multivariate calibration for analysis of paracetamol, Propyphenazone and caffeine
Rohman, Abdul; Universitas Gadjah Mada, Faculty of Pharmacy, Department of Pharmaceutical Chemistry
Dzulfianto, Arief; Faculty of Pharmacy, Sanata Dharma University
Riswanto, Florentinus Dika Octa; Faculty of Pharmacy, Sanata Dharma University
UV-spectrophotometry, partial least square, paracetamol, caffeine, and Propyphenazone
The reference method for simultaneous analysis of drugs is chromatography, however, this technique is expensive, complex, and needs excessive sample preparation; therefore, some simple methods like UV spectroscopy is proposed. Assisted with multivariate calibration, it is possible to analyze drugs using UV spectroscopy without prior separation. This study is intended to use UV spectroscopy coupled with multivariate calibration of partial least square (PLS) for simultaneous analysis of paracetamol (PCT), propyphenazone (PROPI), and caffeine (CAFF) in tablet dosage form. The calibration model is prepared by developing a series 20 mixture of PCT, PROPI and CAFF with certain composition randomly and its absorbance is measured at wavelength of 220-313 nm with an interval of 3 nm. The performance of calibration model was assessed by coefficient of determination (R2), root mean square error of calibration (RMSEC) and root mean square error of cross validation (RMSECV). The R2 values for the correlation between actual values of PCT, PROPI and CAFF and predicted values using UV-spectroscopy combined with PLS are 0.9994; 0.9878; and 0.9919, respectively. The calibration errors expressed with RMSEC are 0.027%, 0.082% and 0.043% for PCT, PROPI and CAFF, respectively. While, during cross validation using “leave one out” technique, RMSECV values obtained are 0.062%, 0.095% and 0.982%, respectively for PCT, PROPI and CAFF. The level of drugs obtained are 226.76 ± 14.49 mg/tablet (equivalent to 90.70% from labeled claim) for PCT, 135.74 ± 11.23 mg/tablet (equivalent to 90.49% from labeled claim) for PROPI and 51.69 ± 2.35 mg/tablet (equivalent to 103.38% from labeled claim) for CAFF.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-12-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1250
10.14499/indonesianjpharm28iss4pp191
Indonesian Journal of Pharmacy; Vol 28 No 4, 2017; 191
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1250/851
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1899
2021-01-07T01:00:39Z
3:ART
ANTI-STRESS ACTIVITY OF SOME PLANTS EXTRACTS OF THE NORTH CAUCASUS FLORA
Pozdnyakov, Dmitry Igorevich; Pyatigorsk Medical and Pharmaceutical Institute
Adzhiahmetova, Similla
Chervonnaya, Nadezhda
Mamleev, Andrey
Oganesyan, Eduard
stress; plant extracts; mitochondria
To date, stress is a common medical and socially significant disease that requires rational pharmacotherapeutic correction.The anti-stress properties of ethnolic and aqueous extracts obtained from leaves of Ribes nigrum L., inflorescences of Gaillardia pulchella Foug., stems of Lysimachia punctata L were studied in this work. Acute stress was modeled by immobilization of rats for 2 hours. The test-extracts were administered per os prophylactically in a dose of 1/20 of LD50 (2000 mg/kg). The following parameters were evaluated: organs mass coefficient (adrenal glands, thymus, spleen), the number of stomach erosion; the biochemical changes in the blood serum (adrenaline, cortisol, total protein and glucose concentration); the mitochondrial function parameters in brain and myocardium (evaluation of mitochondrial pore transitional permeability opening and mitochondrial membrane potential). 70% ethanol extract from Gaillardia pulchella Foug. inflorescences has the highest anti-stress activity, the course application of which contributed to the normalization of the weight index of organs, a decrease in glucose concentration by 64.5% (p <0.05), and cortisol by 73.7% (p <0.05) and adrenaline - 78.9% (p <0.05) in the blood, an increase in total protein level by 62.5% (p <0.05) and stabilization of mitochondrial function. The study showed a high anti-stress activity of 70 % ethanol extract from Gaillardia pulchella Foug. inflorescences.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-08-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1899
10.14499/indonesianjpharm31iss2pp131
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 131
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1899/953
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/675
2020-01-27T02:53:16Z
3:ART
ISOLATION AND IDENTIFICATION OF FLAVONOIDS FROM Sesamum indicum
Sharma, Priyanka; Laboratory of Bioactive Compounds and
Algal Biotechnology Department of Botany,University of Rajasthan, Jaipur- India.
302004
Sarin, Renu; Laboratory of Bioactive Compounds and
Algal Biotechnology Department of Botany,University of Rajasthan, Jaipur- India.
302004
Natural substances have long served as sources of therapeutic drugs. Many substances have been derived from traditional medicines. The plants are rich in secondary metabolites. The medicinal properties of these plants have been attributed to the biochemicals present in the plant materials. In addition to their role in human and animal nutrition, knowledge of micronutrients and phytochemical composition is fundamental to the understanding of modes and mechanisms of action of medicinal plants in general. In the present investigation, quercetin and kaempferol have been isolated and identified from stem, leaves and unorganized cultures of Sesamum indicum and maintained by frequent subculturings on Murashige and Skoog’s medium (1962) supplemented with NAA+BAP(5.0+0.5mg/L). The study showed that maximum content of quercetin and kaempferol was observed in 6 weeks old calli and minimum in stem of S. indicum. The structure of the isolated compound was established on the basis of physical, chemical test and spectroscopic evidences.Key words: Flavonoids, quercetin, kaempferol, Sesamum indicum
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/675
10.14499/indonesianjpharm23iss3pp135-140
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 135-140
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/675/541
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1465
2019-10-28T01:20:02Z
3:ART
The Application of Multiplate Resazurin Reduction Assay in The Screening for Anti-Mycobacterial Activity from Indonesian Medicinal Plants
Sari, Martha; Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI)
Syahputra, Gita; Laboratory for Applied Genetic Engineering and Protein Design, Biotechnology Research Center
Kusharyoto, Wien; Laboratory for Applied Genetic Engineering and Protein Design, Biotechnology Research Center
Anti-mycobacterial, phytochemical extracts, Indonesian medicinal plants, MRRA
Tuberculosis (TB) is an airborne illness generated by Mycobacterium tuberculosis (Mtb), also one of the prominent infectious killers of adults worldwide. There is a pressing need to expand novel anti-mycobacterial drugs because of the increasing resistance of pathogenic mycobacteria to existing antibiotics. Native compounds acquired from microbial resources and medicinal cultivars have played an essential part as the origin of TB medications. The microplate resazurin reduction assay (MRRA) is generally utilized to assess natural and synthetic compounds for anti-mycobacterial activity. In our work, the MRRA method was employed to evaluate the anti-mycobacterial activity of extracts from curative plants using Mycobacterium smegmatis and Mycobacterium bovis BCG and to compare them to rifampicin as an anti-mycobacterial drug. The optimized MRRA utilized 2% aqueous DMSO and 62.5 μg/mL resazurin as an indicator compound in 5% aqueous Tween 80. The optimal incubation time for M. smegmatis was 24 hours, and for M. bovis BCG was 48 hours. The methanolic plant extracts were acquired from various Indonesian medicinal plants known to have anti-mycobacterial activity. The MRRA method using M. smegmatis or M. bovis BCG as anti-mycobacterial targets offers a distinct advantage such as low-cost, rapid, and safe screening for anti-mycobacterial activity in a middle to high-through-put-format.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ)
Indonesia Institute of Sciences (LIPI)
2019-08-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1465
10.14499/indonesianjpharm30iss3pp199
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 199
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1465/911
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/659
2020-01-27T02:53:58Z
3:ART
FORMULATION OF NANOCURCUMIN USING LOW VISCOSITY CHITOSAN POLYMER AND ITS CELLULAR UPTAKE STUDY INTO T47D CELLS
Chabib, Lutfi; Departement of Pharmacy, Faculty of Mathematics and science Universitas Islam
Indonesia 55584, Yogyakarta
Martien, Ronny; Departement of Pharmaceutics, Faculty of
Pharmacy, Universitas Gadjah Mada,
Yogyakarta, Sekip Utara Yogyakarta 55281, Indonesia
Ismail, Hilda; Departement of pharmaceutical Chemistry,
Faculty of Pharmacy, Universitas Gadjah Mada,
Sekip Utara Yogyakarta 55281, Indonesia
Using of curcumin as anti cancer agent is restricted by its low solubility, therefore it has low bioavability. This obstacle can be solved by the development of curcumin nanoparticle. Nanoparticle technology has been started to be developed as an alternative solution to improve drug delivery pofile, especially for the less bio-available chemical. This study was aimed to develope nanocurcumin formulation with low viscosity chitosan as the matrix and to study its ability to be taken into the cells in vitro. Method used in the formulation of nanocurcumin in this study is by ionic gelation followed by freeze drying. Entrapment Efficiency then assayed, and its stability was tested by incubating the formula into artificial intestinal fluid (AIF). Furthermore, its toxicity was evaluated, also its cellular uptake ability into T47D cell line. It was found that the Entrapment Efficiency in acetate buffer at pH 4 is higher than at pH 5. This formula also has a good stability in AIF. For the cellular uptake study through fluorescence microscope, it was found that the complex has an ability to penetrate cellular membrane into the cytosol. The cytotoxicity study tell us that the nanocurcumin is non-toxic to normal cell line. For the characterization of the nanoparticles, the average size of this particle is 269.8 nm, its zeta-potential is +18.63 mV, with spherical particle morphology. From the result ofthis study, it is concluded that formulation of nanocurcumin using low viscosity chitosan polymer as the matrix has a great potential as an alternative for anticancer therapy.Key words: nanoparticle, curcumin, low viscosity chitosan, T47D cell line.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/659
10.14499/indonesianjpharm23iss1pp27-35
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 27-35
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/659/525
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1362
2018-11-02T06:46:53Z
3:ART
Formulation of Insulin Self Nanoemulsifying Drug Delivery System and Its In Vitro-In Vivo Study
Winarti, Lina; Department of Pharmaceutics, Jember University, Indonesia
Suwaldi, Suwaldi; Dept of Pharmaceutical Technology Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
Martien, Ronny; Dept of Pharmaceutical Technology Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
Hakim, Lukman; Dept of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
SNEDDS, insulin, D-optimal mixture design, in vitro diffusion study, in vivo study
Particulate delivery system can be used for improving the efficacy of protein and peptide drug. In addition to a polymer-based particulate delivery system, self-nanoemulsifying drug delivery system (SNEDDS), a lipid-based delivery system, is currently developed for either less water-soluble or soluble drugs. This study aims to design SNEDDS for oral insulin administration and its in vitro-in vivo study. The SNEDDS template was designed using D-optimal mixture design and was analyzed using software Design Expert 7.1.5. The obtained optimum template was loaded with insulin and evaluated for its transmittance percentage, emulsification time, particle size, zeta potential, stability, the amount of insulin in vitro diffused across rat intestine, and insulin serum concentration after oral administration. The study results revealed that the optimum template of SNEDDS formula consisted of 10% (w/w) Miglyol 812N, 65% (w/w) Tween 80, and 25% (w/w) propylene glycol. These optimum template then was loaded with insulin and characterized. SNEDDS insulin has particle size of 12.0±1.7 nm, zeta potential of +0.16mV, transmittance of >90%, and emulsification time of < 60 seconds. The stability study showed that SNEDDS insulin was stable from both precipitation and phase separation. The amount of insulin transported from SNEDDS formula in vitro was 32.45±2.03% and non-SNEDDS formula was 10.44±5.04%. In vivo study of SNEDDS insulin produced a significantly increased Cmax, AUC, and F value than insulin non SNEDDS (p < 0.05). In brief, SNEDDS formulation in this study is a promising approach to increase the effectiveness of oral insulin. Insulin is better given orally in SNEDDS formulation than in non SNEDDS formulation.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Ministry of Research, Technology and Directorate of Higher Education of The Republic Indonesia, Faculty of Pharmacy Universitas Gadjah Mada
2018-10-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1362
10.14499/indonesianjpharm29iss3pp157
Indonesian Journal of Pharmacy; Vol 29 No 3, 2018; 157
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1362/877
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/689
2020-01-27T02:53:31Z
3:ART
PHYTOCHEMICAL STUDIES OF Acacia catechu
Baluja, Shipra; Department of Chemistry, Saurashtra University, Rajkot-360005 (Gujarat), INDIA
Chanda, Sumitra; Department of Biosciences, Saurashtra University, Rajkot-360005 (Gujarat), INDIA.
Solanki, Asif; Department of Biosciences, Saurashtra University, Rajkot-360005 (Gujarat), INDIA.
Kachhadia, Nikunj; Department of Biosciences, Saurashtra University, Rajkot-360005 (Gujarat), INDIA.
Acacia catechu Willd. belongs to the family Mimosaceae. It is well known for the powder of wood known as Kathha” in India, which has wide applications. In the present work, the dry wood powder is extracted in various solvents such as 1,4-dioxane, methanol, dimethyl formamide (DMF), acetone, ethanol and distilled water. The phytochemical and physicochemical properties of these various solvent extracts were studied. The phyto constituents present in the different extracts were saponins, tannins, sugars, terpenes, catechu-tannic acid. Alkaloids were present in organic solvents only. The antibacterial activity of all the extracts in DMSO was studied against five bacterial strains but none of the extracts inhibit any of the studied bacterial strains.Key words: Acacia catechu, 1,4-dioxane, methanol, dimethyl formamide (DMF), acetone, ethanol, distilled water, phytochemical analysis, physicochemical analysis.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/689
10.14499/indonesianjpharm23iss4pp238-247
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 238-247
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/689/555
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1863
2022-10-26T01:05:53Z
3:ART
Effects of Peel Extract from Citrus reticulata and Hesperidin, A Citrus Flavonoid, on Macrophage Cell Line
Ikawati, Muthi’
Armandari, Inna
Khumaira, Annisa
Ertanto, Yogi
Citrus reticulata, hesperidin, macrophage RAW 264.7 cell, proliferation, interleukin-10 (IL-10)
The extract of Citrus reticulata has been studied for its biological activities, due to its citrus flavonoid content. The extract and its flavonoid compounds exhibit growth inhibition properties in several cancer cell lines and in vivo models. Conversely, the extract can also induce cell proliferation and angiogenesis, and shows estrogenic effects, in vitro and in vivo. Because of the contrasting effects that depend on the concentration or dosage, the precise action of the extract and its flavonoids need to be elucidated in various cell types. The objective of this study is to evaluate the effect of Citrus reticulata peel extract (Citrus extract) and hesperidin, a citrus flavonoid, on the modulation of cell proliferation in the RAW 264.7 macrophage cell line. Cell viability under Citrus extract or hesperidin treatment was assessed by using the MTT assay. The expression of interleukin-10 (IL-10), an anti-inflammatory cytokine, modulated by Citrus extract was also examined by immunostaining. Low concentrations of Citrus extract at 1 and 100 μg/mL were able to induce cell proliferation, though not significantly, as shown by cell viability of 138 and 114%, respectively. At higher concentrations of 500, 750, and 1000 μg/mL, Citrus extract decreased cell viability significantly by up to 64, 46, and 36%, respectively. Accordingly, hesperidin at low (3.1 μg/mL−61.1 μg/mL) or high (152.6 μg/mL−305.3 μg/mL) concentrations increased or reduced cell viability significantly by up to 116−136% or 10−61%, respectively. The value of the 50% inhibitory concentration (IC50) of Citrus extract was more than three times higher (756 μg/mL) than that of hesperidin (203 μg/mL = 332 μM). Additionally, 250 μg/mL of Citrus extract was able to induce IL-10 expression compared with the control. These results demonstrate that Citrus extract and hesperidin exert a biphasic effect on macrophage cells. The future development of Citrus extract as a co-chemotherapeutic, anticancer, or immunomodulatory agent should include careful consideration of its biphasic effect on each cell type.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-02-19
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1863
10.14499/indonesianjpharm30iss4pp260
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 260
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1863/935
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1417
2019-10-10T01:29:33Z
3:ART
Nanocurcumin Preparation for Reducing Vcam-1 and IL-6 in High Fat Diet-Induced Hyperlipidemic Rats
Pradana, Dimas Adhi; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia Jl. Kaliurang, km. 14,5 Yogyakarta 55584 Indonesia
Ardhi, Maulana; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia Jl. Kaliurang, km. 14,5 Yogyakarta 55584 Indonesia
Hasyono, Arifa Caryn Dea Utami; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia Jl. Kaliurang, km. 14,5 Yogyakarta 55584 Indonesia
Meytasari, Dzihni; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia Jl. Kaliurang, km. 14,5 Yogyakarta 55584 Indonesia
Nabilah, Farah Deastasa
Istikharah, Rochmy
Chabib, Lutfi
Atherosclerosis, curcumin, SNEDDS, nanosuspension, nanoemulsion, solid lipid nano, VCAM-1, IL-6, preventive measure
Hyperlipidemia is a pathological condition due to lipid metabolism abnormalities. Increased and oxidized LDL in hyperlipidemia will trigger an inflammatory response and produce proinflammatory cytokines, such as vascular cell adhesion molecule-1 (VCAM-1) and Interleukin-6 (IL-6). Curcumin can be used as antioxidants, anti-inflammatory and antihyperlipidemia, but as it is practically insoluble in water, formulation of curcumin nanosuspension is made to improve the effects of curcumin therapy. This study was conducted to determine the activities of nanocurcumin preparation as preventive measures for rats induced with hyperlipidemia. The test animals used were 49 male Wistar rats divided into 7 test groups: normal control group, negative control group, 80mg/kg BW/day curcumin control group, and nanosuspension curcumin dose of 80mg/kg BW/day, nanoemulsion curcumin dose of 80mg/kg BW/day, SNEDDS curcumin dose of 80mg/kg BW/day and solid lipid nanoparticles dose of 80mg/kg BW/day. Provision of preventive measure was performed on days 1-67. On day 11-67, the induction of lard and egg yolk (1:1) was administered with a volume of 2mL/200g of rat BW. On the 68th day, blood samples were taken for the determination of VCAM-1 and IL-6 parameters using Biotin-Streptavidin-Amplified Enzyme-Linked Immuno-sorbent Assay method. Data analysis of VCAM-1 and IL-6 levels between test groups were conducted by means of normality test and One-way ANOVA (p<0.05). Based on the data of VCAM-1 and IL-6 levels, nanocurcumin dose of 80mg/kg BW preparation was able to significantly improve the preventive activity of curcumin compared to curcumin suspension dose of 80mg/kg BW by reducing VCAM-1 levels in SNEDDS (53.260%), nanoemulsion (52.737%), nanosuspension (52.325%) and solid lipid nanoparticles (51.444%) and decreasing IL-6 levels in SNEDDS (33.030%), nanoemulsion (31.568%), nanosuspension (29.898%), and solid lipid nanoparticles (28.875%).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Directorate of Research and Community Service of the Universitas Islam Indonesia, Laboratory of Pharmacology and Laboratory of Pharmaceutical Technology Department of Pharmacy Universitas Islam Indonesia
2019-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1417
10.14499/indonesianjpharm30iss1pp58
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 58
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1417/894
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1236
2018-01-09T08:39:08Z
3:ART
DEVELOPMENT AND EVALUATION OF CONTROLLED RELEASE FORMULATION OF LAMIVUDINE BASED ON MICROPOROUS OSMOTIC TABLET TECHNOLOGY USING FRUCTOSE AS OSMOGEN
Sahoo, Chinmaya Keshari; Pharmaceutics Department, Faculty of Pharmacy, University College of Technology, Osmania University, Hyderabad, Telangana-500007
Rao, Surepalli Ram Mohan; Mekelle Institute of Technology, Mekelle University, Mekelle, Ethiopia
Sudhakar, Muvvala; Department of Pharmaceutics, Malla Reddy College of Pharmacy, Maisammaguda, Secunderabad, Telangana-500014
AIDS; wet granulation; in vitro drug release; stability study
The present study was undertaken to develop controlled release osmotic pump tablets of lamivudine a nucleoside reverse transcriptase inhibitor for the treatment of acquired immune deficiency syndrome (AIDS).The tablets were prepared by wet granulation method using controlled release polymer hydroxyl propyl methyl cellulose (HPMCE5 LV), MCC as diluent, starch as binder and fructose as osmogen. The coating solution of core tablets were prepared by using cellulose acetate,poly ethylene glycol 400,600,4000,6000 and acetone to quantity sufficient with sorbitol for different batches. The prepared tablets were evaluated for pre compression parameters, post compression parameters, in vitro drug release study and scanning electron microscopy study. Among the prepared formulations LF4 batch show 97.78% drug release in 12hrs.The in vitro release kinetics were analyzed for different batches by different pharmacokinetic models such as zero order, first order,Higuchi,Korsmeyer Peppas and Hixon Crowell model. Short term stability study at 40±2ºC/75±5% RH for three months on the best formulation was performed showing no significant changes in thickness, hardness, friability, drug content and in vitro drug release.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-08-22
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1236
10.14499/indonesianjpharm28iss3pp167
Indonesian Journal of Pharmacy; Vol 28 No 3, 2017; 167
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1236/834
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1810
2021-01-07T01:00:39Z
3:ART
QuEChERS and C18 as solid phase extraction sorbent - ultra-high performance liquid chromatography -ultraviolet-visible method for determination of parabens in cosmetics products
Nguyen, Ngoc-Van Thi; Can Tho University of Medicine and Pharmacy
paraben, cosmetic, QuEChERS, solid phase extraction
Concerns are growing about human exposure to endocrine-disrupting chemicals (EDCs), especially during the preadolescent development stage. Parabens are prevalent EDCs widely used as additives in cosmetics. So, the determination of parabens in such products is important. In this study, we developed a reliable and sensitive method to determine simultaneously nine common parabens (methylparaben, ethylparaben, phenylparaben, benzylparaben, penthylparaben, and two groups of isomeric compounds include propylparaben, isopropyl paraben, and butylparaben, isobutylparaben) in cosmetics products. The QuEChERS and solid-phase extraction techniques are used for extraction parabens from non-surfactant cosmetics (perfume, mouth wash solution) and surfactant cosmetics (shampoo, cream, gel), respectively and quantified by using ultra-performance liquid chromatography coupled with the ultraviolet-visible detector. All nine compounds showed good linearity with regression coefficients predominantly above 0.990. The LOD and LOQ of parabens were 0.07 µg/mL; 0.2 µg/mL, respectively. The recoveries ranged from 80 to 110% with the relative standard deviations below 8%. The developed method was successfully applied to determine parabens in various commercial cosmetic products from a local supermarket and the total parabens concentrations are in a wide-ranged from 2.0 to 1270 mg/kg.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-08-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1810
10.14499/indonesianjpharm31iss2pp69
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 69
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1810/950
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/667
2020-01-27T02:51:49Z
3:ART
THE OPTIMISED CONDITIONS OF INDUCTION OF RECOMBINANT RIP rMJC15310 ACTIVITY ISOLATED FROM Mirabilis jalapa L. LEAVES
Astuti, Puji; Department of Pharmaceutical Biology,
Faculty of Pharmacy Universitas Gadjah Mada,
Sekip Utara, Yogyakarta, 55281.
., Sudjadi; Department of Pharmaceutical Chemistry, Faculty of Pharmacy Universitas Gadjah Mada, Sekip Utara, Yogyakarta, 55281
., Sismindari; Department of Pharmaceutical Chemistry, Faculty of Pharmacy Universitas Gadjah Mada, Sekip Utara, Yogyakarta, 55281
Ribosome Inactivating Proteins (RIPs) are compounds isolated from plants with ability to inhibit protein synthesis. The inhibition of protein synthesis is due to inactivation of ribosomal RNA through a site-specific deadenylation mediated by RNA N-glycosidase. Reportedly, RIPs mainly possess wide range of bioactivity including antiviral activity against plant infections. Other activities of RIP were as abortifacien, antivirus and anticancer. This study was aimed to isolate and characterize the optimum conditions for inducing the expression of recombinant RIPs isolated from the leaves of Mirabilis Jalapa L. We have been successfully isolated several RIPs and engineered these proteins to be expressed in E. coli. These recombinant proteins were obtained by screening cDNA library originated from the mRNA of Mirabilis jalapa L leaves, and inserted into pUC19 carrying lacZ gene. The presence of recombinant plasmid was tested by using α-complementation assay. Many RIPs have been isolated from plants and these proteins express enzymatic activity by cutting supercoiled double stranded DNA. One RIP namely rMJC15310 was obtained from this study and the proteins having ~ 8kb in size, cut the supercoiled DNA into linear form at the concentration as low as 5 µg. The ability to cut supercoiled DNA increased on inducing its expression with 0.4% IPTG.Key words: Ribosome Inactivating Proteins (RIP), IPTG, Mirabilis jalapa L., recombinant protein
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/667
10.14499/indonesianjpharm23iss2pp93-98
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 93-98
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/667/533
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1464
2019-10-10T01:30:23Z
3:ART
Citalopram and Tianeptine: Pharmacological Interventions in alcohol withdrawal-induced negative mood states and serotonin insufficiency in Albino Wistar male rats
Amjad, Iffat Ara; Minhaj University Lahore
Abdul Rub, Samina Bano; University of Karachi
Serotonin, Alcohol withdrawal syndrome, Serotonergic antidepressants. Tryptophan metabolism
ABSTRACT The present study aims to investigate the neurochemical and behavioural effects of tianeptine and citalopram to block alcohol withdrawal-induced audiogenic seizures in alcohol withdrawal (AW) rats. Citalopram and tianeptine were administered sub-chronically to block AW-induced audiogenic seizures. Brain regional tryptophan (TRP), 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA) concentrations were determined using high-performance liquid chromatography connected to the fluorimetric detector. Citalopram increased brain TRP concentration in all the regions, however, tianeptine increased brain TRP concentration only in amygdala and hippocampus. Increased brain serotonin concentration was seen by citalopram but not by tianeptine in all the regions. Further, tianeptine was shown to increase 5-HT turnover in all the regions, however, citalopram appeared to increase 5-HT turnover only in the hippocampus. It is concluded that the citalopram and tianeptine behave differently on the intrinsic pathway of serotonin metabolism that appeared to be compensated by the release pattern of serotonin. Further, chronic exposure of serotonergic agents causes restoration and structural reforming of serotonin reuptake mechanism that is desensitized following chronic alcohol exposure. Thus the inclusive approach of the serotonergic system plays an undoubted role in the pharmacological management of AW syndrome.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Higher Education Commission,Pakistan
2019-06-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1464
10.14499/indonesianjpharm30iss2pp75-83
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 75-83
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1464/906
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1318
2018-10-23T02:19:20Z
3:ART
The Formulation of Pacing (Costus speciosus) Extract Tablet by Using Avicel®Ph 200 As Filler-Binder and Amylum As Disintegration Agent
Damayanti, Damayanti; Faculty of Pharmacy UGM, Yogyakarta
Sari, Ika Puspita; Department Pharmacology and Clinical Pharmacy. Faculty of Pharmacy UGM, Yogyakarta
Sulaiman, T.N. Saifullah; Department Pharmaceutical, Faculty of Pharmacy UGM, Yogyakarta
Bestari, Angi Nadya; Department Pharmaceutical, Faculty of Pharmacy UGM, Yogyakarta
Setiawan, Irfan Muris; Department Pharmacology and Clinical Pharmacy. Faculty of Pharmacy UGM, Yogyakarta
Keywords: tablet, Avicel® PH 200, amylum, Costus speciosus
Pacing (Costus speciosus) is an herbaceous plant that is native to Indonesia and it can be used as a male contraceptive due to spermatogenesis inhibition. The purpose of this study is to find out the composition of optimum Avicel PH 200® as the filler-binder and amylum as the disintegration agent and to find out the variations on physical properties of the powder and tablet. The tablets were made by directly compression in 8 runs based on Simplex Lattice Design (SLD) from Design Expert 7.1.5, in which the test on physical properties of powder includes tapping index, water absorption, and moisture content, as well as on the physical properties of tablet, including hardness, friability, and disintegration time. The results showed that the variation in the composition between Avicel® PH 200 as the filler-binder and amylum as the disintegration agent had a significant effect on the friability of Costus speciosus (CS) extract tablet, in which the combination of both materials can increase the friability of the tablet. The optimum formula of CS tablet had a composition of Avicel® PH 200 by 462,5 mg and amylum by 37,5 mg contained in each tablet.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-01-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1318
10.14499/indonesianjpharm29iss1pp29
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 29
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1318/849
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1340
2018-10-23T02:19:33Z
3:ART
The Effect Of Temperature On Recombinant Human Granulocyte Colony Stimulating Factor Production By Pichia pastoris Expression System
Adiredja, Yuliawati
Fuad, Asrul Muhamad
granulocyte colony stimulating factor , complex and minimal medium, temperature, Pichia pastoris.
Granulocyte colony stimulating factor (G-CSF) is a haematopoetic growth factor that functions as specific stimulator of the survival, proliferation, and differentiation of precursor cells of the neutrophilic granulocyte cell lineage as well as an activator of mature neutrophil function. The main objective of this work is to compare the effect of different temperature on the production of extracellular recombinant G-CSF in Pichia pastoris. Cells were cultured for 72h in baffled shake-flasks at 20°C, 25°C, and 30°C in two different medium; buffered glycerol/methanol-complex medium (BMGY/BMMY) and buffered minimal glycerol/methanol (BMGH/BMMH) after methanol induction every 12h. Expressed recombinant hG-CSF in the methylotrophic yeast P. pastoris was analyzed with SDS-PAGE. The 23 kDa protein was secreted into the culture supernatant when induced with methanol. Production of recombinant G-CSF protein in P. pastoris at 30°C at 48h incubation after methanol induction every 12h is the highest in both complex and minimum medium.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-07-10
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1340
10.14499/indonesianjpharm29iss2pp94
Indonesian Journal of Pharmacy; Vol 29 No 2, 2018; 94
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1340/865
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/684
2020-01-27T02:53:31Z
3:ART
PHYSICAL PROPERTIES OF WOUND HEALING GEL OF ETHANOLIC EXTRACT OF BINAHONG (Anredera cordifolia (Ten) Steenis) DURING STORAGE.
Yuliani, Sri Hartati; Faculty of Pharmacy, Universitas Gadjah Mada,
Skip Utara, Yogyakarta 55281 Indonesia
Faculty of Pharmacy, Universitas Sanata Dharma, Paingan, Maguwoharjo, Depok, Yogyakarta 55284 Indonesia
Fudholi, Achmad; Faculty of Pharmacy, Universitas Gadjah Mada,
Skip Utara, Yogyakarta 55281 Indonesia
Pramono, Suwijiyo; Faculty of Pharmacy, Universitas Gadjah Mada,
Skip Utara, Yogyakarta 55281 Indonesia
., Marchaban; Faculty of Pharmacy, Universitas Gadjah Mada,
Skip Utara, Yogyakarta 55281 Indonesia
Binahong (Anredera cordifolia (Ten) Steenis) has been used as wound healing in traditional Indonesian medicine. The developing of the dosage forms using the formulation technology approach has been done. The aim of this study was to examine the physical properties of the wound healing gel of ethanolic extract of binahong during storage. The factorial design method 3 factors and 2 levels was employed to achieve this study. The three factors used in this study were Carbopol, CMC-Na and Caalginate with low and high level for each factor. The physical properties of the wound healing gel of ethanolic extract of binahong was evaluated on day 1, 30, 60 and 90 for viscosity and bioadhesion study. The result showed that CMC-Na and Caalginate increased the alteration of physical properties of wound healing gel of ethanolic extract of binahong during storage. The carbopol maintained the physical properties of the gel during storage.Key words: bioadhesion, binahong, viscosity, wound healing gel.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/684
10.14499/indonesianjpharm23iss4pp203-208
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 203-208
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/684/550
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1698
2022-10-26T01:05:53Z
3:ART
Pharmacoeconomic Rationale of Zinc Supplementation In The Management of Acute Diarrhea In Children With Rotavirus Infection In Indonesia
Hakim, Mohamad Saifudin; Department of Microbiology, Faculty of Medicine, Public Health and Nursing
Soloviov, Serhii
Soenarto, Yati; Department of Chemistry IPB University, Bogor
Dzyublyk, Iryna
Leleka, Maryia
Trokhymchuk, Viktor
iarrhea, modelling, rotavirus, treatment, zinc supplementation
Rotavirus is one of the most important causative agents of gastroenteritis in both infants and children worldwide, resulting in a high mortality and burden of disease, mainly in low-income, developing countries. Oral rehydration therapy with zinc supplementation is currently the recommended way to prevent death from diarrheal dehydration. In this study, we aimed to estimate the effectiveness of zinc supplementation in combination therapy of patients with suspected rotavirus infection in Indonesia. The perspective of this pharmacoeconomic study refer to the economic vantage point of cost-of-illness analysis. Achievement of this goal was possible with the construction of a decision tree model and determination of decision rules for inclusion of zinc supplementation into the combination therapy. The input parameters of the model were hospitalization days of patients stratified by the presence or absence of rotavirus infection as well as the additional inclusion of zinc supplementation. The criterion for prediction and decision making was the global rotavirus prevalence. The feature of the simulation was that the costs were expressed as relative to each other, which allowed to unify the proposed methodology. Retrospective analysis of clinical database of Indonesian patients with acute diarrhea has shown that zinc supplementation would be rational in case of rotavirus prevalence among these patients is higher than 81.5%. It was shown that additional zinc supplementation would be costeffective with probability of 0.62 and 0.53 – in the positive range of cost ratio variation or if costs for less than 43% of baseline therapy per day costs on an average.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Directorate of Research and Community Service, Ministry of Research Technology and Higher Education of the Republic of Indonesia for financial support of this research by Penelitian Fundamental Grant 2019 (No: 4167/IT3.L1/PN/2019).
2020-01-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1698
10.14499/indonesianjpharm30iss4pp285
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 285
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1698/926
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/507
2020-02-19T02:37:07Z
3:ART
SEDATIVE EFFECT OF CLARY SAGE (SALVIA SCLALAREA, L) ON MICE USING AN OLFACTORY AROMATHERAPY
Hadinoto, Idajani; Faculty of Pharmacy
Unika Widyamandala Surabaya
Kuswono, Engkun; Faculty of Pharmacy
Unika Widyamandala Surabaya
Marlina, Ani; Faculty of Pharmacy
Unika Widyamandala Surabaya
Setiawati, Anna; Faculty of Pharmacy
Unika Widyamandala Surabaya
A research has been conducted to study the sedative effect of the Clary sage oil (Salvia sclaria, L), using an olfactory aromatherapy method, on male white mice, which have been tested using rotarod, platform and chimney tests. Clary sage oil was given to the male white mice in three different exposed times : 0.5, 1, and 1.5 hours. As a reference standard was used diazepam 170 ug/kg body weight (intraperitoneal). The research indicates that the administration of Clary sage oil can indeed cause the sedative effect. There is also a correlation between the exposure time of Clary sage oil and the sedative effect observed. Key-words : sedative effect, clary sage oil, olfactory aromatherapy
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/507
10.14499/indonesianjpharm0iss0pp44-51
Indonesian Journal of Pharmacy; Vol 12 No 1, 2001; 44-51
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/507/385
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1105
2018-01-09T08:38:31Z
3:ART
Antiinflamatory and antidepressive activities of Extract Curcuma xanthorrhiza Roxb in Systemic Lupus Erythematosus
Setiawati, Maria Caecilia; STIFAR Yayasan pharmasi Semarang
Ikawati, Zulies
Kertia, I Nyoman
Systemic Lupus Erythemathosus is an autoimmune, inflammatory, chronic disorder characterized by multiorgan system involvement. Depression and anxiety are frequent complaints among patients with lupus erythematosus, so antidepressive treatment should be an important element of the therapy of patients with lupus erythematosus. Research into curcumin’s potential as a treatment for depression is still in its infancy, although several potential antidepressant mechanisms of action have been identified. 4-weeks double-blind, placebo-controlled clinical trial was conducted in 14 SLE patients (10 as treatment group and 4 as control group) The BDI (Beck Depression Inventory) score was calculated and TNF α concentration was measured in the serum subjects before and after treatment. Correlation between TNF α concentration and BDI was assessed. After 4 weeks treatment, the TNF α concentration from subjects in treatment group were significantly lower than before ( p <0.001) but the BDI score were not significantly lower than before (p = 0.059). The TNF α concentration and BDI score showed positive correlation, p = 0,024. This study demonstrate that Curcuma xanthorrhiza Roxb extract can decrease serum TNF α concentration and reduce clinically symptoms of depression in SLE patients
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-08-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1105
10.14499/indonesianjpharm28iss3pp185
Indonesian Journal of Pharmacy; Vol 28 No 3, 2017; 185
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1105/836
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1691
2021-01-06T13:13:53Z
3:ART
An Assessment of Cough Medicine Dispensing Practice to Children Under Two Years Old in Pharmacies in Ho Chi Minh City Using Simulated-Patient Method
Phan, Quyen Thi Ngoc; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Truong, Van Thi; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Pham, Thanh Ngoc; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Nguyen, Ho Nhu; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Department of Pharmacy, Nguyen Trai Hospital, Vietnam
Bui, Quynh Thi Huong; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Department of Pharmacy, Thong Nhat Hospital, Vietnam
Cough medications, Children, Pharmacy
Over-the-counter (OTC) cough and cold medications (CCMs) have been used to treat the symptoms of upper respiratory infection in children for decades. The safety of CCMs in children has been questioned. The data on knowledge of pharmacists in supplying cough medicines for children under two years have been limited. This study aimed to evaluate the pharmacists’ dispensing decisions to manage the cough in children under two years old. A descriptive cross-sectional was carried out in 300 pharmacies in 15 districts in Ho Chi Minh City, Vietnam. The pharmacists were interviewed by a simulated patient. The results showed that, information that pharmacists actively asked the client about the patient and disease symptoms was limited. Most pharmacists did not provide adequate instructions and counsel about using drugs for clients. Only 22/300 (7.33%) of pharmacists appropriately provided cough medicines for children under 2 years old. The main reason of inappropriateness was the deficiency of knowledge about updated contraindication of N-acetylcysteine (93.17%). Pharmacists in pharmacies located in districts 3, 11 and Binh Thanh had higher rate of rational provision than those in other districts. A good and full understanding of the patient symptom helped the pharmacists supply cough medicines more reasonably. The limited caution of pharmacists and the low proportion of pharmacists updating contraindication of N-acetylcysteine should be considered as a warning sign in pharmacy practice in Ho Chi Minh City, Vietnam.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-04-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1691
10.14499/indonesianjpharm31iss1pp42
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 42
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1691/943
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1691/265
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/662
2020-01-27T02:53:58Z
3:ART
SELAGINELLA ACTIVE FRACTIONS INDUCE APOPTOSIS ON T47D BREAST CANCER CELL
Handayani, Sri; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Risdian, Chandra; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Meiyanto, Edy; Faculty of Pharmacy, Universitas Gadjah
Mada, Indonesia. Skip Utara 55281
Yogyakarta
Udin, Zalinar; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Andriyani, Rina; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Angelina, Marissa; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Apoptosis is an important target on anticancer mechanism. The purpose of this research is to investigate apoptosis induction of Selaginella plana Hieron active fractions on T47D cells. Absolute ethanol was used to extract Selaginella plana powders. Ethanolic extract was dilluted by methanol:water (4:1) and then fractionated by hexane (S_Hex), methylene chloride (S_MTC), ethyl acetate (S_EA), and buthanol (S_BuOH). The proliferation of T47D cell line was detected by SRB (Sulforhodamine B) assay which was measured at a wavelength of 515nm. Flowcytometry analysis to determine apoptosis was examined by Propidium Iodide (PI) and Annexin V assay using T47D breast cancer cell line. The result showed that the IC50 value of S_Hex, S_MTC, S_EA, and S_BuOH on T47D cells were 107 µg/mL, 4 µg/mL, 6 µg/mL, and 17 µg/mL respectively. The active fractions (S_MTC and S_EA) at its IC50 concentration significantly (P<0.05) increased the total number of early apoptotic cells in the T47D cells 3.39% and 4.1% respectively compared to that of control (1.95%). Based on the result, methylene chloride and ethyl acetate fraction of Selaginella plana induced apoptosis on T47D cell.Keywords: apoptosis, breast cancer, Selaginella
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/662
10.14499/indonesianjpharm23iss1pp48-53
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 48-53
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/662/528
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1439
2019-10-10T01:30:23Z
3:ART
Immunomodulatory Activity of Yogurt Fortified with Honey and Rosella (Hibiscus sabdariffa L.) on Reactive Oxygen Intermediate (ROI) and Nitric Oxide (NO) Secretion
Mahfudh, Nurkhasanah; Faculty of Pharmacy, Universitas Ahmad Dahlan
Novitasari, Rina; Faculty of Pharmacy, Universitas Ahmad Dahlan
Immunomodulator, Yoghurt, Rosella (Hibiscus sabdariffa L), ROI, NO
Raise the body's immune system. Rosella (Hibiscus sabdariffa Linn.) is known to have anthocyanin compounds that have antioxidant and immunomodulatory effects. The aim of this research is to know the effect of immunomodulator yogurt which fortified by rosella (Hibiscus sabdariffa L.) extract to increase the secretion of Reactive Oxygen Intermediate (ROI) and Nitric Oxide (NO). This research was conducted in vivo using 25 male mices with Balb/C strain divided into 5 groups: normal group, plain yogurt treated group and 2%, 4%, 8% rosella yogurt treated group. Treatment was given for 21 days orally. On the 22nd day the mice were sacrificed by taking peritoneum macrophage cells and then tested the secretion of Reactive Oxygen Intermediate (ROI) and Nitrite Oxide (NO). The results showed that there was an increase of ROI and NO secretion in 2%, 4%, and 8% rosella yoghurt treated groups compare to plain yoghurt. The fortification of yoghurt with rosella extract and honey increase the potency of yoghurt in increasing the immunomodulatory activity
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-05-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1439
10.14499/indonesianjpharm30iss2pp141-146
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 141-146
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1439/901
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1309
2018-10-23T02:18:57Z
3:ART
Effect of Lipid Ratio of Stearic Acid and Oleic Acid on Characteristics of Nanostructure Lipid Carrier (NLC) System of Diethylammonium Diclofenac
Hendradi, Esti; Department of Pharmaceutics, Faculty of Pharmacy Airlangga University.
Jl. Dharmawangsa No.4-6, Airlangga, Gubeng, Kota SBY, Jawa Timur 60286
Rosita, Noorma; Department of Pharmaceutics, Faculty of Pharmacy Airlangga University.
Jl. Dharmawangsa No.4-6, Airlangga, Gubeng, Kota SBY, Jawa Timur 60286
Rahmadhanniar, Erwinda; Department of Pharmaceutics, Faculty of Pharmacy Airlangga University.
Jl. Dharmawangsa No.4-6, Airlangga, Gubeng, Kota SBY, Jawa Timur 60286
DETA, NLC, physicochemical characterization, entrapment efficiency
The aim of this study was to determine the effect of lipid ratio of stearic acid and oleic acid on the physical characteristics as well as the entrapment efficiency of diethylammonium diclofenac with Nanostructure Lipid Carrier (NLC) system. Diethylammonium diclofenac (DETA) is Non-Steroid Anti-Inflamatory Drugs (NSAIDs) that has been widely used in the treatment of osteoarthritis and rheumatoid arthritis. In the formulation of NLC-DETA, three different lipid ratios were used, which the ratio of stearic acid:oleic acid were 60:40, 70:30, and 80:20, respectively. In this NLC system, DETA served as the active drug, stearic acid as solid lipid, oleic acid as liquid lipid, and Tween 80 as surfactant components. NLC were characterized for organoleptic characteristics, pH, viscosity, particle morphology, particle size and polydispersity index (PI), profiles of Fourier Transform Infra-Red (FTIR) and Differential Thermal Analysis (DTA), and drug entrapment efficiency. The particle shape and morphology were determined by Transmission Electron Microscopy (TEM). The results showed that the different ratios of oleic acid lipids and stearic acid had no significant effects on the viscosity and entrapment efficiency of NLC-DETA. On the other hand, it affected the pH of all formulas, which were significantly different. Increasing the amount of liquid lipid in the formulations reduced the size of NLC-DETA particles.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-10-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1309
10.14499/indonesianjpharm28iss4pp198
Indonesian Journal of Pharmacy; Vol 28 No 4, 2017; 198
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1309/852
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1731
2020-05-30T04:59:18Z
3:ART
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/679
2020-01-27T02:53:16Z
3:ART
QSAR MODELING OF 2-[CH(OH)X]-5,8-(OY)2 -1,4-NAPHTHOQUININES AGAINST L1210 CELLS USING MULTIPLE LINEAR REGRESSION
., Ajeet; Department of Pharmaceutical Chemistry,
S. D. College of Pharmacy and Vocational Studies, Muzaffarnagar, U.P. 251001
India
Singh, Brajpal; Department of Pharmaceutical Chemistry,
S. D. College of Pharmacy and Vocational Studies, Muzaffarnagar, U.P. 251001
India
Kumar, Vipul; Department of Pharmaceutical Technology, Meerut Institute of Engineering and Technology, Meerut, U.P., India
Quinones are present in many drugs such as anthracyclines, daunorubicin, doxorubicin, mitomycin, mitoxantrones and saintopin, which are used clinically in the therapy of solid cancers. The cytotoxic effects of these quinone are mainly due to the inhibition of DNA topoisomerase-II. It is the necessity to develop the 1,4-Naphthoquinone analogues with Cytotoxic effect. Here 2-[CH(OH)X]-5,8-(OY)2-1,4-Naphthoquinines analogues have been used to correlate the cytotoxic activity with the Eccentric Connectivity index (ECI), Fragment Complexity (FC) and McGowan Volumes (MG) for studying the Quantitative Structure Activity Relationship (QSAR). Correlation may be an adequate predictive model which can help to provide guidance in designing and subsequently yielding greatly specific compounds that may have reduced side effects and improved pharmacological activities. We have used Multiple Linear Regression (MLR), one of the best methods for developing the QSAR model. Results from this QSAR study have suggested that ECI, FC and MG are the important descriptors for cytotoxic activities of 1,4-Naphthoquinones against L1210 cells. For the validation of the developed QSAR model, statistical analysis such as data point-descriptor ratio, fraction of variance, cross validation test, standard deviation, quality factor, Fischer’s test; and internal validation such as Y-randomization test have been performed and all the tests validated this QSAR model.Key words: 1,4-Naphthoquinones, QSAR, Eccentric connectivity index, Fragment complexity, McGowan Volume, Multiple Linear Regression
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/679
10.14499/indonesianjpharm23iss3pp171-176
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 171-176
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/679/545
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1492
2022-10-26T01:05:53Z
3:ART
Quantitative Analysis of Multi-components in Curcuma xanthorrhiza by Single Marker
Badrunanto, Badrunanto
Wahyuni, Wulan Tri
Nirwati, Hera
Rafi, Mohamad
C. xanthorrhiza; curcuminoids; QAMS; relative coefficient factor
A new simple and effective routine analytical method for quantification of curcuminoids in Curcuma xanthorrhiza was developed by high-performance liquid chromatography. This method based on chromatographic fingerprint combined with a quantitative analysis of multi-components by single marker (QAMS). Curcumin was selected as an internal marker for the determination of two other similar compounds, i.e. bisdemethoxycurcumin and demethoxycurcumin, by using the relative coefficient factor (RCF). Excellent linearity was obtained for each component (r2 > 0.9998), and the recovery of extraction methods were within 100.23-103.95%. The precision of the method was good at inter-day and intra-day analysis (RSD < 4.0%). The stability of RCFs was good under various chromatographic conditions with RSD < 1%, and the ratio of retention time was used to locate each compound. The quantification of curcuminoids between QAMS and external standard method (ESM) proved the consistency and similarity of the two method (RSD < 2%). This study demonstrated that QAMS could be used as a routine method for quality control of curcuminoids in C. xanthorrhiza. This method successfully proved accurate, stable, more effective and simple than external standard method.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-10-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1492
10.14499/indonesianjpharm30iss4pp301
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 301
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1492/921
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/440
2020-02-19T02:37:07Z
3:ART
PROTOPLAST SUSPENSION CULTURE OF LEAVES MESOPHYLOF Centella asiatica (L.) Urban AND QUALITATIVE ANALYSIS ASIATICOSIDE
Prihastanti, Erma; Faculty of Science and Mathematic, Diponegoro University
Semarang
Soegihardjo, C. J.; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
Purbaningsih, Susiani; Faculty of Biology
Universitas Gadjah Mada
Yogyakarta
Centella asiatica leaves has been used as diuretic, antihypertensi, anti leprae, skin infection, burning skin and celloid. Cells suspenson culture of mesophyll of Centella asiatica leaves were carried out in three steps: isolation, purification, and cell culture. The aim of this research is to investigate the influences of leaves position (age of leaves) and concentration of macerozyme R-10 for cells number and viability, and the influence of sucrose concentration for cells growth and biosynthesis of asiaticoside. Isolation and purification of mesophyll cell has been performed using factorial completely randomized design. The first factor was leaf position (age of leaves), and the second factor was concentration of macerozyme. Collected data were analysed using Anova and Duncan’s test at 0.0 – 2.5 – 5.0 and 7.5 per cent respectively. Asiaticoside produced by cell biomass were analyzed qualitatively using thin layer chro- matography. The result revealed that mesophyll cells could be used as explant for suspension culture. The highest biomass produced with highest viability were found in cells isolated from second leaves treated with 0.1% macerozyme (1.32 . 107 cells/ml). Addition of sucrose to suspension culture medium produced higher packed cells volume (pcv) percentage than no sucrose was added, (0% sucrose gave pcv 8.5%, 2.5% sucrose pcv 22%, sucrose 5% pcv 21.5%, and 7.5% sucrose pcv 15.75%). The asiaticoside production not only depended on sucrose, because without any sucrose was added, the asiaticoside was also available..Key Words: Cell suspension culture, mesophyll, Centella asiatica (L.) Urban, asiaticoside
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/440
10.14499/indonesianjpharm0iss0pp10-19
Indonesian Journal of Pharmacy; Vol 12 No 1, 2001; 10-19
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/440/319
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1425
2019-10-03T03:16:50Z
3:ART
Efficacy of Thymol and Eugenol Against Polymicrobial Biofilm
Hamzah, Hasyrul; Faculty of Pharmacy, Universitas Gadjah Mada
Tunjung Pratiwi, Sylvia Utami
Hertiani, Triana; Faculty of Pharmacy, Universitas Gadjah Mada
Thymol, Eugenol, polymicrobial biofilm, MBIC50, MBEC50
Biofilms associated with human infection have high levels of pathogenicity due to their resistance to antibiotics. The discovery of an active antibiofilm agent against polymicrobial biofilms is a necessary consequence for coping with biofilm-related infections. Thymol and Eugenol are essential oils that have potential as antibacterial and antifungal. This study aimed to determine the effectiveness of thymol and eugenol inhibits C. albicans, P. Aeruginosa, E. coli S. aureus and polymicrobial biofilm. Biofilm formation inhibition assay and biofilm degradation assay of thymol and eugenol were determined using microtiter broth method.The antibiofilm efficacy of thymol and eugenol towards polymicrobial biofilms were analyzed by calculating minimum biofilm inhibitor concentration (MBIC50) and minimum biofilm eradication concentration (MBEC50) values. The data were analyzed using Statistical Package for the Social Sciences (SPSS) with 95% confidence level. Thymol and eugenol showed inhibitory activity against the formation of mono and polymicrobial biofilms of the microbial tested.The result also demonstrated an evidence of activity of thymol and eugenol in breaking down mono and polymicrobial biofilm. Therefore, thymol and eugenol serves as a potential source for new antibiofilm drugs towards polymicrobial biofilm.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-12-17
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1425
10.14499/indonesianjpharm29iss4pp214
Indonesian Journal of Pharmacy; Vol 29 No 4, 2018; 214
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1425/886
Copyright (c) 2018 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/513
2018-09-05T01:49:55Z
3:ART
SYNTHESIS AND ANTIMICROBIAL ACTIVITY EVALUATION OF SOME SCHIFF BASES DERIVED FROM 2-AMINOTHIAZOLE DERIVATIVES
Gupta, Rajul; Dept. of Pharmacy, CMJ University, Modrina Mansion, Laitumkhrah, Shillong, Meghalaya-793003, India.
Fuloria, Neeraj Kumar; Dept. of Pharmaceutical Chemistry, Anuradha College of Pharmacy, Chikhli, Dist. Buldana, Maharashtra, 443201, India.
Fuloria, Shivkanya; Dept. of Pharmaceutical Chemistry, Anuradha College of Pharmacy, Chikhli, Dist. Buldana, Maharashtra, 443201, India.
Various substituted acetophenones (1-5) on treatment with iodine and thiourea yielded 2-amino-4-(substituted-phenyl)thiazole (1a-5a), which on further treatment with various substituted aldehydes to get N-(substitutedbenzylidene)-4(substitutedphenyl)thiazol-2-amine (1b-5b). All the synthesized compounds were characterized by their respective FTIR, H NMR and Mass data. Synthesized compounds (1b-5b) were subjected to investigation for their antibacterial and antifungal studies against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Asperigillus flavus and Asperigillus fumigatus by disk diffusion method. Compound 5b was found to be most effective with largest zone of inhibition. Key words: Thiazole, Acetophenones Antibacterial, Antifungal, Substituted Aldehydes.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-09-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
text/html
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/513
10.14499/indonesianjpharm24iss1pp35-39
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 35-39
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/513/391
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/513/912
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1512
2019-10-10T01:29:33Z
3:ART
Conjugation of Anti-EpCAM Antibody on Alginate–RIP MJ-30 Nanoparticle through Carbodiimide Reaction as a Model of Targeted Protein Therapy
Ismail, Hilda; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia – 55284
Ciptasari, Ummi H.; Undergraduate Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia - 55284
Ikhsan, M Arief Nur; Undergraduate Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia - 55284
Suryani, Fidya; Undergraduate Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia - 55284
Sismindari, Sismindari; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia – 55284
Martien, Ronny; Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia – 55284
Yuswanto, Ag; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia – 55284
Ribosome inactivating proteins from Mirabilis jalapa L. (RIP MJ) has shown higher cytotoxic activity when being formulated as a nanoparticle. However, the selectivity of the delivery system is also an important aspect when it comes to cytotoxic cell therapy. Epithelial cell adhesion molecule (EpCAM) is a monomeric glycoprotein which is overexpressed in epithelial cancer cells. This study aim was to develop a model of targeted protein delivery system by formulating the base fraction of RIP MJ (RIP MJ-30) into alginate nanoparticles and conjugating it with anti-EpCAM antibody. RIP MJ-30 was formulated in to nanoparticle using alginate and CaCl2 as cross-linker. Optimization of conjugation reaction condition was done in the pH variation of 4.5, 5.5, and 6.5. The success of conjugation was analyzed qualitatively using native polyacrylamide gel electrophoresis (native-PAGE) method and BCA assay. The optimum formula of RIP MJ-30 nanoparticles was produced using 0.3% alginate and 0.2% CaCl2. Results indicated that optimum conjugation reaction was carried out at pH level of 5.5. The optimum native-PAGE condition was by using 8% polyacrylamide gel in duration of 6h. Characterization of nanoparticle resulted in particle size of 205.0nm, zeta potential of -6.9mV, entrapment efficiency of 71.11±4.84%, and conjugation efficiency of 89.55±6.18%. It was concluded that RIP MJ-30 was successfully formulated into alginate nanoparticle and conjugated to anti-EpCAM antibody through carbodiimide reaction using 1-ethyl-(dimethylprophilamine) carbodiimide (EDAC).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-03-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1512
10.14499/indonesianjpharm30iss1pp52
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 52
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1512/893
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1274
2018-10-23T02:18:57Z
3:ART
The Influence of Medication Days’ Supply on Adherence and Cost of Hypertensive Patients at Primary Health Care Centre
Mufarrihah, Mufarrihah; Faculty of Pharmacy, Universitas Airlangga
Setiawan, Catur Dian
Wathaniah, Nurul
Rahmaniah, Atikah
Maharani, Primanda Ayu
Mahmudah, Azzahroh Sifa’ Lailiyah
Achmad, Gusti Noorrizka Veronika
adherence, cost, hypertension, medication days’ supply, Primary Health Care Centre
Primary Health Care Centre is known have policy discrepancy with the supply of medicines. This can influence the adherence and costs incurred by patients. The study aimed to identify the influence for hypertensive patients at Primary Health Care Centre in Surabaya. This study was designed cross sectional and data collection was done by using ARMS questionnaires for the patient's adherence and interview for the cost. Cost was calculated based on the frequency of real visits and monthly patient expenditures. Direct medical cost, direct non medical cost, and indirect cost were included based on patients’ perspective. Direct observations were done to calculate the days of drug supplies. A total of 406 patients from 50 Health Care Centre were included in the study. Days of drug supplies ranged from 3 to 30 days; total ARMS scores ranged from 12 to 33; and the total cost obtained ranged from IDR 34,965 to IDR 239,528. The Spearman’s correlation test sought to obtain the significant influence of medication days’ supply on adherence with p value 0.0001 and r = -0.347. While medication days’ supply didn't show any significant influence on the cost. It will be significant if the patient met the visit schedule, that was p value 0.0001 and r = -0.247. It is recommended to prolong antihypertensive days’ supply in order to improve patients' adherence, as well as to reduce cost expensed by patients.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-12-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1274
10.14499/indonesianjpharm28iss4pp213
Indonesian Journal of Pharmacy; Vol 28 No 4, 2017; 213
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1274/854
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1257
2018-06-22T02:08:58Z
3:ART
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/673
2020-01-27T02:51:49Z
3:ART
PASSIVE AND IONTOPHORETIC PERMEATION OF CAPTOPRIL GEL: AN IN VITRO AND IN VIVO STUDY
Jain, Ashish; Bansal College of Pharmacy, Kokta, Anand Nagar, Bhopal-462021, India.
Nayak, Satish; Bansal College of Pharmacy, Kokta, Anand Nagar, Bhopal-462021, India.
Soni, Vandana; Department of Pharmaceutical Sciences, Dr H.S.Gour Vishwavidyalaya, Sagar 470003, M.P. India
The Objective of this work was to formulate and evaluate captopril gel to assess its suitability for transdermal delivery by passive and iontophoresis. A polymer gel was prepared using hydroxypropyl methyl cellulose and in vitro skin permeability was assessed in full thickness skin of rabbits and pigs. For in vivo studies New Zealand rabbits were used. In vitro passive permeation was carried out in Franz diffusion cell but for iontophoresis, diffusion cell was modified according to Glikfield design. Iontophoresis was performed at a current density of 0.5 mA/cm2via silver /silver chloride electrodes with passive controls but for in vivo study current density wasreduced to 0.1 mA/cm2. Blood samples were analyzed for drug content by HPLC. Results of the in vitro study indicated that iontophoresis considerably increased the permeation rate of captopril compared to passive controls in both the skin types (P<0.01). The plasma concentration of captopril was significantly higher (P<0.001) than that obtained in the passive controls. Results showed that the target permeation rates for captopril could be achieved with the aid of iontophoresis by increasing the area in an appreciable range.Key words: Captopril, iontophoresis, transdermal, Rabbit, Pigskin, in vitro, in vivo.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/673
10.14499/indonesianjpharm23iss2pp121-129
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 121-129
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/673/539
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1545
2019-10-28T01:20:02Z
3:ART
Effect of 7-Hydroxy-2-(4- Hydroxy -3-Methoxyphenyl)-Chroman-4-one On Level of Mangan-Superoxide Dismutase (Mn-Sod) and Superoxide Dismutase 2 (Sod2) Gene Expression in Hyperlipidemia Rats
Ayunda, Rahmah Dara; Magister of Biomedical Science Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada
Prasetyastuti, Prasetyastuti; Departement of Biochemistry Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada
Hastuti, Pramudji; Department of Biochemistry Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada, Skip Utara 55281 Yogyakarta
Hyperlipidemia, flavonoids, Mn-SOD, SOD2 gene
Hyperlipidemia is a lipid metabolism disorder characterized by an increase in serum lipid levels. Hyperlipidemia is a major risk factor for many metabolic syndrome diseases because it triggers oxidative stress. Oxidative stress can be reduced by endogenous antioxidant enzymes triggered by exogenous antioxidant compounds, such as 7-OH-2- (4-OH-3-methoxyphenyl)-chroman-4-one isolated from the seeds of Swietenia macrophylla King. The aims of this study were to investigate the effects of 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one compounds on cholesterol level, LDL level, Mn-SOD levels and SOD2 gene expression of hyperlipidemic rats. Thirty rats (Rattus norvegicus ) were divided into 6 groups, normal group (N), hyperlipidemia group (HL), hyperlipidemia group with simvastatin (P), hyperlipidemic group with 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one with dose 10 (F10), 30 (F30) and 90 (F90) mg/200g body weight (BW). Cholesterol and LDL were analyzed with CHOD-PAP method, Mn-SOD level was analyzed by ELISA method and SOD2 gene expression was analyzed by qPCR method. The decrease in cholesterol and LDL levels were most prevalent in group F90 with dose 90 mg/200g BW of 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one, with average difference each of them was 172.43 mg/dL and 36.12 mg/dL. The rats fed on high-cholesterol diet exhibited a significant elevation in Mn-SOD levels (p<0.05) compared to normal group. The treated animals with 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one has the level of Mn-SOD is significantly lower (p<0.05) compared with hyperlipidemic group. Expression of SOD2 in group F90 has value close to normal group (p> 0.05). 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one with dose of 90 mg/200g BW improved cholesterol levels, LDL levels, Mn-SOD levels and SOD2 gene expression in hyperlipidemic rats.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-08-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1545
10.14499/indonesianjpharm30iss3pp180
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 180
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1545/915
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/658
2020-01-27T02:53:58Z
3:ART
HEPATOPROTECTIVE EFFECT OF GAMAVUTON-0 AGAINST D˗GALACTOSAMINE/LIPOPOLYSACCHARIDE-INDUCED FULMINANT HEPATIC FAILURE
Nurrochmad, Arief; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta 55281,
Indonesia
Sari, Ika Puspita; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta 55281,
Indonesia
Murwanti, Retno; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta 55281,
Indonesia
., Sardjiman; Curcumin Research Center, Faculty of Pharmacy, Universitas Gadjah Mada
Yogyakarta 55281, Indonesia.
Candraningrum, Triana; Curcumin Research Center, Faculty of Pharmacy, Universitas Gadjah Mada
Yogyakarta 55281, Indonesia.
Afritasari, Dyah; Curcumin Research Center, Faculty of Pharmacy, Universitas Gadjah Mada
Yogyakarta 55281, Indonesia.
Martina, Devina; Curcumin Research Center, Faculty of Pharmacy, Universitas Gadjah Mada
Yogyakarta 55281, Indonesia.
Siahaan, Iren Wati; Curcumin Research Center, Faculty of Pharmacy, Universitas Gadjah Mada
Yogyakarta 55281, Indonesia.
The objective of this study is to determine the hepatoprotective effect of GVT-0 (one of curcumin analogues) against liver damage in rat-induced D-galactosamine (D-GalN)/lipopolysaccharide (LPS) as a model of fulminant hepatitis. In the study D˗GalN/LPS elevated serum GPT activity that indicate a particular occurrence of liver damage due to depletion of UTP and UDP-glucuronic acid. Administration of GVT-0 (10 mg/kg) showed decreased enzyme activity of SGPT/SGOT but had no effect on serum ALP and total bilirubin levels, whereas at doses of 20 and 40 mg/kg, the protective effect of GVT-0 was decrease. The glutathione content in the D-GalN/LPS (0.76 ± 0.07) mol/g liver content was found lower than controls (0.90 ± 0.03) mol/g liver. Administration of GVT-0 dose of 10, 20 and 40 mg/kg restored glutathione content returned to normal levels. The results showed that treatment of GVT-0 showed no effect on TBARS and catalase activity. Treatment of D-GalN/LPS, indicating the trend of increased TNF-α, although statistically not significant, while the administration of GVT-0 showed a tendency to decrease the concentration of TNF-α. All findings of the results indicated that the GVT-0 mainly lower dose (10 mg/kg) showed hepatoprotective action in rat model of fulminant hepatitis induced by D-GalN/LPS. The results indicated that the mechanism of hepatoprotective effect of GVT-0 is not via antioxidant properties of GVT-0. However, further studies are necessary to explain the molecular mechanism of hepatoprotective effect of GVT-0.Key words: Gamavuton-0, hepatoprotective, fulminan hepatitis, D˗galactosamine/LPS
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/658
10.14499/indonesianjpharm23iss1pp18-26
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 18-26
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/658/524
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1383
2018-11-02T06:46:53Z
3:ART
Determination of Sitagliptin Levels in Rats Serum by HPLC and its Pharmacokinetic Investigation in Existence of Sucralose
Dayyih, Wael Abu; Department of Pharmaceutical Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy and Medical Sciences, University of Petra, Queen Alia International Road, Amman - Jordan 11196, Jordan
Hamad, Mohammed; Department of Basic Sciences, Collage of Sciences and Health Professions, King Saud Bin Abdulaziz University for Health Sciences, Jeddah 21423, Saudi Arabia
sitagliptin, sucralose, tadalafil, pharmacokinetic, interaction
It is an interest of the current work to develop a simple, valid and rapid chromatographic method for the quantification of sitagliptin in rats' serum. Accordingly, the pharmacokinetic parameters of sitagliptin was investigated for any possible interaction between sitagliptin and sucralose. The developed HPLC method was validated for sitagliptin determination in rat’s serum. Results indicated an accomplished overall intra-day precision and accuracy values of 0.139-4.028 (CV%) and 99.48-107.12% respectively. On the other hand, inter-day precision and accuracy results recorded values of 0.150-2.810 (CV%) and 99.90-116.00% respectively. Moreover, the coefficient of correlation was determined at a value of 0.99950 with reasonable sensitivity and selectivity. Effect of sucralose showed strong significant correlation on sitagliptin serum profile. The precise, accurate, linear, sensitive and selective HPLC method will be elaborated in the context of the current work with emphasis on sucralose negative effect on sitagliptin level in rat’s serum. Such finding is a guidance for physicians to reconsider the use of such sweeteners for diabetic patients
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-09-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1383
10.14499/indonesianjpharm29iss3pp117
Indonesian Journal of Pharmacy; Vol 29 No 3, 2018; 117
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1383/873
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/690
2020-01-27T02:53:31Z
3:ART
DEVELOPMENT THE VALIDATION OF INDONESIAN VERSION OF SF-36 QUESTIONNAIRE IN CANCER DISEASE
Perwitasari, Dyah Ariani; Faculty of Pharmacy, Ahmad Dahlan University
Jl. Kapas No.9, Semaki, Yogyakarta, Indonesia
55166.
A Cancer patients need long term therapy in the treatment of their disease. One of the outcome of cancer treatment in patients of quality of life. Because of the limited instrument for measuring cancer patients’ quality of life in Indonesian version, thus this study is aimed to develop the validation of Indonesian version of Short Form-36 questionnaire in cancer patients. The observational study was carried out in this study. Data were collected from cancer patients in the Oncology Department of Sardjito Hospital, Yogyakarta, Indonesia, who were treated with cisplatin at the dosage of ≥ 50mg/m2 as monotherapy or in combinations. The validation process involved known-group validity, discriminant validity, convergent validity and factor analysis. About 203 subjects who were diagnosed with cancer were recruited in this study. Theknown-group validity test showed that there were no significant differences of SF-36 domains among different diagnosa. All of the questions met the criteria of convergent validity except for the questions number 26, 28, 30 and 32. Furthermore, the questions number 2 and 28 did not meet the criteria of discriminant validity. This study presents that many questions are needed to be reconstructed, due to the result of factor analysis which showed that those questions are loaded significantly with other domain’s questions. The development of Indonesian version of SF-36 scales in cancer disease is still less unstatisfactory. According to the result of this study, future study with new construction of questions in this version is still necessary.Key words: validation, SF-36, Indonesia, cancer
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/690
10.14499/indonesianjpharm23iss4pp248-253
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 248-253
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/690/556
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1529
2021-01-06T13:13:53Z
3:ART
Inhibition of Cell Cycle and Induction of Apoptosis y Ethanol Leaves Extract of Chrysanthemum cinerariifolium (Trev.) In T47D Breast Cancer Cells
Mutiah, Roihatul; Department of Pharmacy, Faculty of Medicine and Health Science, Maulana Malik Ibrahim Islamic State University of Malang
Inayatin, Alfiyah Laily; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
Annisa, Rahmi; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
Indrawijaya, Yen Yen Ari; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
Listiyana, Anik; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
C. cinerariifolium, apoptosis, cell cycle, T47D cells
Chrysanthemum cinerariifolium (C.cinerariifolium) is a plant of the Asteraceae family, which has been applied by the community as an ornamental plant and traditional medicine. In this study, the effect of C. cinerariifolium leaves extract on inhibition of cell cycle and induction of apoptosis in T47D breast cancer cells was tested and compared to the standard chemotherapy agent. The citotoxic activity of C. cinerariifolium leaves extract against T47D cancer cells and Vero normal cells was tested by MTT method. Profile of apoptosis and cell cycle were observed by flow cytometry method. Based on chemical compounds profil which is tested used TLC showed that C.cinerariifolium leaves extracts contained flavonoid and terpenoid chemical compounds. The result of cytotoxic test showed that leaves extract of C. cinerariifolium was able to inhibit the growth of T47D cancer cell at IC50 418.8μg/mL. Doxorubicin, extracted from Streptomyces peucetius used as treatment in several cancers including breast cancer. Doxorubicin could inhibit the growth of T47D cancer cells in 115.1μg/mL. The results of cell cycle analysis showed that the C. cinerariifolium leaves extract inhibited cell cycle in G0-G1 and S phase, whereas doxorubicin was able to inhibit cell cycle in G0-G1 phase but experienced cell accumulation in G2-M phase. The percentage of apoptosis in cycle was showed in M1 (sub G1) and M5 (multinuclear) phase which treatment of C. cinerariifolium leaves extract was higher than doxorubicin. Therefore, C. cinerariifolium leaves extract has potential activity as anticancer agent causes inhibition of cell cycle and induction apoptosis.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
This work supported by The Directorate General of Islamic Higher Education (DIKTIS) of Interdisciplinary Basic Research Grant numbers 3209/Un.3/HK.00.5/05/2018.
2020-04-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1529
10.14499/indonesianjpharm31iss1pp1
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 1
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1529/937
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1349
2019-10-10T01:29:33Z
3:ART
The Properties of Brown Marine Algae Sargassum turbinarioides and Sargassum ilicifolium Collected From Yogyakarta, Indonesia
Artemisia, Rahma; Stikes Madani Yogyakarta
Jl. Wonosari KM 10, Karanggayam, Sitimulyo, Piyungan, Bantul, DIY
Nugroho, Akhmad Kharis; Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
Setyowati, Erna Prawita; Department of Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
Martien, Ronny; Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
Sargassum turbinarioides, Sargassum ilicifolium, Fucoidan, Thin Layer Chromatography (TLC), Fourier transform infrared (FTIR) spectroscopy
Brown marine algae are the prominent source of marine natural products that have bioactive metabolites. Sargassum turbinarioides and Sargassum ilicifolium were dominated in Indonesia as brown marine algae that well known as a source of fucoidan. The samples were collected from Gunungkidul, Yogyakarta, Indonesia. In this study, we investigated and identified the yield of aqueous crude and purified extracts using different extraction temperatures (60°C, 70°C, 80°C, 90 °C). The highest yield of S. turbinarioides crude extract (7.36%) was obtained at temperatures 90 and 80 °C while the highest yield of S. ilicifolium was 3.49 % at 80 °C. The presence of sulfate polysaccharide in Sargassum turbinarioides is 3,78 % and Sargassum ilicifolium is 2,93 %. Each of the extract was screened using phytochemical detection, Thin Layer Chromatography (TLC) and Fourier transform infrared spectroscopy (FT-IR) analysis. The phytochemical detection indicated that Sargassum ilicifolium has bioactive metabolites such as carbohydrates, proteins and amino acids, terpenoid, phenolic compounds, and flavonoids. The FTIR spectrum of the S. turbinarioides and Sargassum ilicifolium extract refer to the presence of ester sulfate groups through showing peaks at 1300 to 1200 cm-1 and 980 to 950 cm-1. The result indicated that Sargassum turbinarioides and Sargassum ilicifolium contain of sulfate polysaccharide were prospect a biological activities to use for the development of marine nutraceutical drugs especially as antioxidant.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Ministry of Research, Technology, and Higher Education, Republic of Indonesia and also Beasiswa Unggulan Dosen Indonesia - Dalam Negeri (BUDI-DN) from Lembaga Pengelola Dana Pendidikan (LPDP) for sponsorship and financial support
2019-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1349
10.14499/indonesianjpharm30iss1pp43
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 43
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1349/892
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1203
2018-01-09T08:39:22Z
3:ART
Chemical composition of rhizome oleoresin and anti-inflammatory, antinociceptive and antipyretic activity of oleoresins of Alpinia allughas Roscoe. from tarai region of Uttarakhand
Kumar, Ravendra; Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar, U.S. Nagar, Uttarakhand, India
Sethi, Sonali; Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar, U.S. Nagar, Uttarakhand, India
Prakash, Om; Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar, U.S. Nagar, Uttarakhand, India
Pant, Anil Kumar; Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar, U.S. Nagar, Uttarakhand, India
Kumar, Mahesh; Department of Vet. Epidemiology and Preventive Medicine, College of Veterinary & Animal Sciences. G.B. Pant University of Agriculture & Technology, Pantnagar, U.S. Nagar, Uttarakhand, India
Isidorov, Valery A.; Institute of Chemistry, Białystok University, Division of Environmental Chemistry, UL. Hurtowa 1,15-399, Białystok, Poland
Szczepaniak, Lech; Institute of Chemistry, Białystok University, Division of Environmental Chemistry, UL. Hurtowa 1,15-399, Białystok, Poland
ABSTRACT The investigation of volatile constituents of the rhizome oleoresin of Alpinia allughas Roscoe. growing in tarai region of Kumaun hills, Uttarakhand, revealed the presence of 55 terpenoids. The major constituents identified in the rhizome oleoresin were α-eudesmol (21.3%), β-selinene (13.9%), valencene (9.6%), borneol (7.1%), α-humulene (5.3%) and 7-epi-α-selinene (5.2%). Other minor constituents identified were (E)-β-caryophyllene (3.8%), (6 E)-nerolidol (3.5%), (E,E)-farnesol (3.2%), caryophyllene oxide (2.5%), humulene oxide (2.7%), bornyl acetate (1.9%), coranarin- E (1.8%), linalool (1.8%) and α-terpineol (1.6%). The total identified constituents contribute 97.0% of the oleoresin. The rhizomes oleoresins exhibited significant antinociceptive activity with 34.79% inhibition at 50 mg/kg body weight and 43.24% at 100 mg/kg body wt. compare to standard drug ibuprofen (40 mg/kg body wt.), it also showed antipyretic activity in dose dependent manner with temperature reduction 77.57±5.88% at 50 mg/kg body wt. and 98.95±3.95% at 100 mg/kg body wt. after 3 hours. Oleoresin also showed 29.23% inhibition in carrageenin-induced paw edema at 50 mg/kg body wt. and 39.92% inhibition at 100mg/kg body wt. in compare to ibuprofen 40.06% at 40 mg/kg body weight.Keywords Alpinia allughas Roscoe.; Zingiberaceae; α-eudesmol; β-selinene; anti-inflammatory; antinociceptive; antipyretic activity; oleoresins
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
UGC, NEW DELHI, INDIA
2017-08-16
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1203
10.14499/indonesianjpharm28iss3pp136
Indonesian Journal of Pharmacy; Vol 28 No 3, 2017; 136
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1203/831
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1203/119
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1988
2021-01-07T01:00:39Z
3:ART
In vitro Comparative Study for Anti-proliferative Activity of Some Plant Extracts, Fam. Apiaceae, on HeLa Cell Line
Gomaa, Sara E.; Medicinal and Aromatic Plants Research Dept., Horticulture Research Institute (HRI), Egypt. 9 ALgamaa Street, Giza, Egypt
Friedersdorf, Matthew; Molecular Genetics and Microbiology Dept., Duke University Medical Center, NC, USA
El Enshasy, Hesham Ali; Director,
Institute of Bioproduct Development (IBD), Universit Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Abou-Donia, MB; Pharmacology and Cancer Biology Dept., Duke University School of Medicine, NC, USA.
Apiaceae - Anti-proliferative - Extracts - HeLa Cell Line
In this research, the biological activities of five plant extracts from family Apiaceae; Italian Parsley (Petroselinum neapolitanum), Fennel (Foeniculum vulgare), Celery (Apium graveolens), Cilantro (Coriandrum sativum) and Dill (Anethum graveolens), were studied. Antiproliferative effect of eleven ethanol crude extracts was tested in Human Cervical (Hela) cancer cells. Results clearly demonstrated that all plant extracts showed high significant difference when compared to the negative control (DMSO).Parsley leaves extract, cilantro leaves extract and cilantro stems extract showed no significant difference with the positive control (Actinomycin D). As for, fennel bulb extracts, fennel stalks extracts, celery stems gave better results than the positive control with no significant difference through the 24, 48 and 72 h treatment. There were no significant difference between Fennel extracts and the positive control, which showed high effect on the cancer cells survival. There were no significant difference between both extracts of Cilantro leaves and stems through each time but the best result was after 72 h of treatments. Regarding Dill leaves and stems, cell numbers recorded no significant difference between the both on time dependent manner. Further investigation for ethanolic extracts of parsley leaves, fennel bulb, fennel stalks, celery stems, cilantro leaves and cilantro stems which showed better results than using the commercial drug Actinomycin D (25ml/ml) for 24 h treatment or less depending on concentrations manner. Also, further investigation on different types of cancer cell lines to avoid the toxic effect of chemotherapy.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Jack Kenee, Duke University, USA
2020-08-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1988
10.14499/indonesianjpharm31iss2pp108
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 108
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1988/946
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/668
2020-01-27T02:51:49Z
3:ART
IN VIVO EVALUATION OF MISOPROSTOL FLOATING MICROSPHERES
Vanitha, Kondi; Vishnu institute of pharmaceutical
education and research, Medak, A.P., India
Varma, Mohan; Shri Vishnu college of pharmacy,
Bhimavaram, A.P.,India
Ramesh, Allure; Vishnu institute of pharmaceutical
education and research, Medak, A.P., India
Prostaglandin (PG) has been reported to be an important protective and acid suppressive factor in the gastric mucosa. The objective of the study was to develop and evaluate a stomach specific drug delivery system for controlled release of Misoprostal a PE analogue for gastric ulcer induced NSAIDs. Floating microspheres were prepared by emulsification-solvent evaporation method using ethyl cellulose as a polymer, carbopol as mucoadhesive polymer. Ulcers were induced by the oral administration of absolute ethanol (5 mL/kg) to 24 h fasted Wistar male rats (n=8), weighing 200 g. Sodium bicarbonate solution, misoprostal solution and drug loaded microspheres were tested. Formulations were administered orally 1h before the administration of ethanol. Prior to the oral administration, rats were anesthetized with ethylic ether. After 2 h of ethanol administration, animals were sacrificed; the stomachs were removed, opened along he greater curvature and examined for lesion measurements. Ulcer indexes (UI) were calculated. The in vivo evaluation showed that ulcer index values were 0.61 ± 0.14 for the sodium bicarbonate solution, 0.58 ±0.18 for the misoprostal and 0.11 ± 0.06 for the misoprostal-loaded microspheres. The KruskalWallis test detected statistical differences (p =0.002) between the ulcer indexes. The multiple analyses (Student-Newman-Keuls) showed that the misoprostal loaded microspheres presented a gastric ulcer index statistically lower than that of the sodium bicarbonate solution (p = 0.001) and the misoprostal solution (p = 0.021). The percentages of ulceration inhibition were 31 and 75% after the administration of misoprostal solution and microspheres, respectively. In conclusion, the in vivo evaluation showed that the microspheres presented ulcer index lower than the solutions, showing that misoprostal-loaded microspheres were efficient in protecting the stomach against ulcer formation.Key words: Misoprostal, microspheres, ulcer index,
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/668
10.14499/indonesianjpharm23iss2pp99-103
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 99-103
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/668/534
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1467
2019-10-28T01:20:02Z
3:ART
Anticancer Molecules from Catharanthus roseus
Taher, Zarani M; Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Agouillal, Farid; 1. Research Unit on Analysis and Technological Development in Environment, URADTE, Centre de Recherche Scientifique et Technique en Analyses Physico-Chimiques, CRAPC, B.P. 384, RP 42004 Zone Industrielle Bou-Ismail, Tipaza, Algeria.
2. Laboratory of Reaction Engineering, LGR, Faculty of Mechanical Engineering and Process Engineering, Houari Boumediene University of Science and Technology, USTHB, PB 32 El Alia, 16075, Bab Ezzouar, Algiers, Algeria.
R, Lim J.; School of Chemical Engineering and Energy, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Marof, Aina Q; Department of Chemistry, Mathematics and Science Faculty, Universitas Negeri Jakarta, Jakarta, Indonesia
Dailin, Daniel Joe; 1. Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
2. School of Chemical Engineering and Energy, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Nurjayadi, Muktiningsih; Department of Chemistry, Mathematics and Science Faculty, Universitas Negeri Jakarta, Jakarta, Indonesia
Razif, Ezzaty NM; Faculty of Applied Sciences, Universiti Teknologi Mara Negeri Sembilan, Kampus Kuala Pilah, Pekan Parit Tinggi 72000, Kuala Pilah Negeri Sembilan, Malaysia
Gomaa, Sara E; Horticulture Research Institute, Agriculture Research Center, Egypt.
El Enshasy, Hesham Ali; Director
Institute of Bioproduct Development (IBD)
Universiti Teknologi Malaysia (UTM)
Skudai, Johor Bahru, Malaysia
C. roseus; Anticancer; Extraction; Phytochemical; Tissue culture
Catharanthus roseus is an important medicinal plant found in various parts of the world and the bioactive compound has been extracted and used as anti-cancer agent to treat the cancer over decades. However, the extraction of bioactive compound also results in the generation of large quantities of pollution with wasted solvents. Toxic pollution occurs when synthetic chemicals are discharged or natural chemicals accumulate to toxic levels in the environment, causing reductions in wildlife numbers, degrading ecosystem functions and threatening human health. This review covers the extraction and phytochemical obtained leading to chemical compounds related to anti-cancer property of C. roseus. Additionally, recent advances of using biological cell cultures were also addressed. Thus, this work can be used for further investigation of C. roseus to be undertaken in future for its anti-cancer property further development and efficient production in drug industry
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
MOE and UTM-RMC (Malaysia) through HICOE grant no. R.J130000.7846.4J262.
2019-07-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1467
10.14499/indonesianjpharm30iss3pp147
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 147
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1467/910
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1308
2018-10-23T02:19:33Z
3:ART
Protective Effect of Ethanolic Extract Tempuyung Leaf (Sonchus arvensis L.) in Gentamicin-induced Acute Tubular Necrosis on Wistar Rats
Imelda, Imelda -; Biomedical Science Master Program, Graduate School of Medical Sciences, Universitas Padjadjaran, Bandung
Achadiyani, Achadiyani -; Department of Anatomy, Physiology and Cell Biology,Faculty of Medicine, Universitas Padjadjaran, Bandung
Sekarwana, Nanan -; Department of Pediatrics, Faculty of Medicine, UNPAD-RSHS, Bandung
acute tubular necrosis, creatinine, gentamicin, tempuyung, Sonchus arvensis L.
Acute tubular necrosis (ATN) is the most common histopathologic findings of acute kidney injury (AKI). AKI is marked by the decrement of glomerular filtration rate, causing waste metabolism retention (creatinine). Gentamicin is used as it is the most common nephrotoxic agent in inducing ATN. Tempuyung (Sonchus arvensis L.) has been used in folklore medicine to ameliorate kidney problems as it contains antioxidants, two of which are flavonoid and phenolic acid. Yet, these active substances’ benefit on gentamicin induced ATN has not been investigated in Indonesia. This research aims to analyze protective effect of ethanolic extract tempuyung leaf (EET) on gentamicin-induced ATN based on histopathological study and creatinine serum level. True experimental laboratoric study was done with simple random design on male wistar rats, randomly divided into 4 groups (n=4). Control group (CMC 0.5% aquadest); Induction group (Gentamicin 80 mg/kgBW); 1st treatment group (EET 100 mg/kgBW + Gentamicin 80 mg/kgBW) and 2nd treatment group (EET 200 mg/kgBW + Gentamicin 80 mg/kgBW) for ten days. On the 11th day, blood was taken for creatinine measurement and kidneys collection for histopathological study. Histopathological examination on gentamicin-treated rats revealed degenerative changes in kidney tubules. Aside from that, gentamicin-treated rats also showed increment in creatinine serum level. Conversely, simultaneous administration of EET with Gentamicin ameliorated the nephrotoxic effects of gentamicin as confirmed from the significant improvement on histopathological changes and normalization of creatinine serum level. Co-administration of EET and gentamicin provides protection on gentamicin-induced ATN, based on histopathological feature and creatinine serum level.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
-
2018-05-11
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1308
10.14499/indonesianjpharm29iss2pp86
Indonesian Journal of Pharmacy; Vol 29 No 2, 2018; 86
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1308/870
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1308/142
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1308/143
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1308/144
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1325
2018-11-02T06:46:53Z
3:ART
Free radical scavenging potential of Drosera indica L in presence of Dalton Ascites lymphoma (DAL) tumor bearing mice
Asirvatam, Raju
Christina, AJM
: Drosera indica L, Antioxidant, Dalton Ascites lymphoma, Malondialdehyde, Protein carbonyl content
The aim of the present study was to evaluate the antioxidant role of Drosera indica L. in Dalton Ascites lymphoma (DAL) bearing Swiss albino mice. The effect of ethanol and aqueous extracts of D.indica (EEDI and AEDI) were administered at a 250 and 500mg/kg once a day for 14 days, 24h after the inoculation of tumor cell line. After the treatment period, blood was collected from the animals and subsequently the animal were sacrificed for isolation of liver, brain, kidney and lungs for the observation of antioxidant status level. The parameters analyzed were catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), peroxidase (P), total protein (TP) and protein carbonyl content (PCC). Treatment with EEDI and AEDI significantly reduced the levels of MDA and increased the levels of GSH, SOD, CAT, P and TP in cancer induced animal and are similar as that of normal mice. The results suggest that the ethanol and aqueous extract of Drosera indica L. possess significant antioxidant effects in DAL bearing mice
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-07-27
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1325
10.14499/indonesianjpharm29iss3pp127
Indonesian Journal of Pharmacy; Vol 29 No 3, 2018; 127
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1325/874
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/685
2020-01-27T02:53:31Z
3:ART
ENHANCEMENT OF DISSOLUTION RATE OF MODAFINIL USING SOLID DISPERSIONS WITH POLYETHYLENEGLYCOLS
Jigar, Vyas; Department of Pharmacy, Bhagwant University, Sikar Road, Ajmer, Rajasthan, India. 305004
Jayvadan, Patel; Nootan Pharmacy College, Visnagar, Gujarat, India. 384315
A., Jain D.; Department of Pharmacy, Bhagwant University, Sikar Road, Ajmer, Rajasthan, India. 305004
Solid dispersions (SDs) of modafinil (MDF) were prepared using polyethyleneglycols (PEGs), in 1;1, 1;2 and 1;4 proportions by fusion, solvent evaporation and physical mixing method. Differential scanning calorimetry (DSC) and X-ray powder diffractometry (XRD) were used to examine the physical state of the drug. The data from the XRD showed that the drug was converted to amorphous form as the number and intensity of peaks were decreased in solid dispersion as compared to pure drug and physical mixture of drug and carrier. DSC thermograms also confirmed the change in physical state of the drug as the peaks were altered or disappeared. With the highest ratio of the carriers (1:4), the drug solubility was enhanced by 38.68, 34.78 and 9.29 folds in solvent evaporation, fusion and physical mixing methods respectively. Solid dispersion batch S6 containing drug:PEG6000 in 1:4, was selected to be formulated as tablet (batch TS6) and evaluated for in vitro drug dissolution & six month stability. An increased dissolution rate of modafinil was observed from SDs and PMs, as compared to pure crystalline drug. The dissolution rate of modafinil from its PMs or SDs increased with an increasing amount of polymer.Key words: Fusion, solvent evaporation, physical mixture, in vitro dissolution, characterization.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/685
10.14499/indonesianjpharm23iss4pp209-215
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 209-215
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/685/551
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1470
2022-10-26T01:05:53Z
3:ART
Quality of Life of Patient with Hypertension in Primary Health Care in Bandar Lampung
Khoirunnisa, Sudewi Mukaromah; Institut Teknologi Sumatera
Akhmad, Atika Dalili; Institut Teknologi Sumatera
Hypertension; Quality of Life; SF-36
The high prevalence of hypertension in Indonesia, encourages studies related to how the quality of life of patients with hypertension. The purpose of this study was to measure quality of life, identify and explain factors related to the quality of life of patients with hypertension.The study design was descriptive correlation using a cross sectional study approach. The study subjects were all outpatient hypertensive patients several health centers in Bandar Lampung. The instrument used in this study was the Indonesian SF-36. The data collected included the patient's demographic characteristics including gender, age, education, occupation, and marital status, and the fields related to the health history of the study subjects included the duration of hypertension, complications, and the number of antihypertensive drugs consumed. Data were analyzed using bivariate analysis to see the relationship between two variables with the level of significance used was 5% (α = 0.05) with the value of the confidence interval set was 95%. Multivariate analysis was conducted to study the relationship of several independent variables with one or several dependent variables.The results of univariate analysis showed that age, marital status, duration of illness, complications, and the number of drugs consumed had an effect on the quality of life of hypertensive patients (p <0.05). The results of multivariate analysis showed that the factors of age, marital status, and duration of hypertension are factors that influence the physical domain, while the factors of gender, marital status, duration of hypertension, complications, and the number of drugs are influential factors in the mental domain mental.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
The Ministries of Research, Technology and Higher Education of Indonesia
2020-01-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1470
10.14499/indonesianjpharm30iss4pp309
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 309
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1470/927
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1470/200
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/508
2020-02-19T02:37:07Z
3:ART
AKTIVITAS PENGANGKAPAN RADIKAL POLIFENOL DALAM DAUN TEH
Rohdiana, Dadan; Tea and Quinine Research Center
Gambung PO BOX 1013 Bandung 40010
Ability of free radical scavengers of various tea extracts (black tea, fermented tea; green tea , unfermented tea; oolong dan pouchong tea semifermented tea) was studied. The scavenging effects on active oxygen decreased in order semifemented tea > unferfmnted tea > fennented lea. Antioxidant activity on tea often might related wthi catechin presence. Catechin activity as antioxidant estabilished. Several epidemilogical and phartnacokgical research shown that catechin had a strongly antioxidan activity. (-)-epigallocatechin gallate (EGCG) and (-)-epicatechin gallate (ECG) are the major catechin of ~ tea This compounds had a strongly scavenging activity on free radical such as superoxide, hydrogen superoxide, DPPH and peroxynitdte. This review aim to exploited~ tea Oyphenols ability as free radicals sw vengers Key word: antioxidant activity, active oxygen, scavenging effect
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/508
10.14499/indonesianjpharm0iss0pp52-58
Indonesian Journal of Pharmacy; Vol 12 No 1, 2001; 52-58
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/508/386
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1235
2018-10-23T02:19:33Z
3:ART
Antimicrobial activity and Identification of fungus associated Stylissa flabelliformis sponge collected from Menjangan Island West Bali National Park, Indonesia
Setyowati, Erna Prawita; Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada
Pratiwi, Silvia Utami Tunjung; Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada
Purwantiningsih, Purwantiningsih; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Universitas Gadjah Mada
Samara, Oka; Faculty of Pharmacy, Udayana University
Sponge-associated fungi, Stylissa flabelliformis, Staphylococcus aureus, Escherichia coli, Candida albicans
The Fungus is a very important microorganism as a producer of bioactive secondary metabolites. Active substances of microbial origin have been sought through the process of screening methods to obtain antimicrobial compounds. The purpose of this study was to isolate fungi associated with sponge taken from Menjangan Island National Park West Bali (Indonesia) and identify fungi that have antimicrobial activity. Isolation of fungus from sponge was carried out by spread plate method using Saboroud Saline Agar medium. Each fungi will be tested to Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922 and Candida albicans ATCC 10231. Identification of fungi is based on the observation of macroscopic, microscopic and also using 16rRNA/ITS phylogeny tree. The results showed that S. flabelliformis sponge had 10 fungal isolates. Most of them have antimicrobial activity. The name associated with a sponge fungus is These 10 fungus are Aspergillus flavus strain UPMZ02, Aspergillus fumigatus strain CD1621, Trichoderma reesei strain JCM 2267, Aspergillus nomius strain KUB105, Aspergillus sp. strain TLWK-09, Aspergillus flavus strain MC-10-L, Penicillium sp. strain RMA-2, Aspergillus sp. strain TLWK-09, Aspergillus fumigatus and Trichoderma reesei strain TV221
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-11-27
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1235
10.14499/indonesianjpharm29iss2pp66
Indonesian Journal of Pharmacy; Vol 29 No 2, 2018; 66
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1235/847
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1903
2021-01-06T13:13:53Z
3:ART
In Vitro Antiplasmodial Activity and Cytotoxicity of Active Subfractions of Harmsiopanax aculeatus Leaves
Turalely, Rachel; Biotechnology Study Program, Graduate School, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Chemistry Education Study Program, Faculty of Teacher Training and Education Science, Pattimura University, Ambon, Maluku, Indonesia
Wijayanti, Mahardika Agus; Department of Parasitology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada,
Hertiani, Triana; Faculty of Pharmacy, Universitas Gadjah Mada
Mustofa, Musthofa; Department of Pharmacology and Therapy, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada Yogyakarta, Indonesia.
H. aculeatus, antiplasmodial activity, cytotoxicity, malaria, subfractions
Harmsiopanax aculeatus leaves, a medicinal plant with locally named kapur, have been used traditionally to treat malaria in Maluku, Indonesia. However, the scientific information of this plant is still limited. In our previous study, the methanol extract of this plant leaves have been proven to possess in vitro antiplasmodial activity. This study was conducted to evaluate in vitro antiplasmodial activity and cytotoxicity of subfractions of the plant leaves. Fractionation was performed using a column chromatography with Sephadex LH-20 as the stationary phase and methanol as the mobile phase. The subfractions obtained were then tested for in vitro antiplasmodial activity on a chloroquine-resistant FCR3 strain of Plasmodium falciparum using a visual method. Cytotoxicity was evaluated by using MTT assay. The in vitro antiplasmodial activity and cytotoxicity were expressed as IC50, calculated using probit analysis with SPSS 16 for windows. The results showed that the four subfractions tested have a high antiplasmodial activity with IC50 values of 0.09; 0.18; 0.01; and 0.77 µg.mL-1, respectively. In addition, these subfractions had IC50 values of >400 µg.mL-1 against Vero cells indicating that they were non-toxic. In conclusion, the subfractions of H. aculeatus leaves are very active and selective against P. falciparum. Further study will be conducted to isolate the active compounds.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-04-09
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1903
10.14499/indonesianjpharm31iss1pp51
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 51
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1903/944
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/663
2020-01-27T02:53:58Z
3:ART
CHROMOSOME CHARACTERIZATION OF THREE VARIETIES OF GINGER (Zingiber officinaleRosc.)
Daryono, Budi Setiadi; Genetics Laboratory, Faculty of Biology
Universitas Gadjah Mada Jl. Teknika Selatan-Sekip Utara Yogyakarta 55281
Rahma, Siti Nur Azizah Fauziati; Genetics Laboratory, Faculty of Biology
Universitas Gadjah Mada Jl. Teknika Selatan-Sekip Utara Yogyakarta 55281
., Purnomo; Laboratory of Plant Systematics, Faculty of
Biology, Universitas Gadjah Mada. Jl. Teknika
Selatan-Sekip Utara Yogyakarta 55281
., Sudarsono; Departement of Pharmaceutical Biology,
Faculty of Pharmacy, Universitas Gadjah Mada,
Sekip, Utara Yogyakarta 55281, Indonesia
Giant ginger (Zingiber officinale Rosc. var. officinale), red ginger (Zingiber officinale Rosc. var. rubra) and small ginger (Zingiber officinale Rosc. var. amarum) are three varieties of Zingiber officinale Rosc. They have a lot of benefit and often used by Indonesian as a traditional drug. Moreover, they have a big chance to be use as a flavor in world wide. Therefore, research for their quality, quantity and continuity of supplyare needed. Characterization of their chromosomes is one effort for improving ginger cultivation. The objective of this research was to study mitotic time and chromosome characters of three varieties of ginger. Squashing method was used for chromosome preparation. The results showed that mitotic time of giant ginger is 09.00-10.05 am, red ginger is 09.00-10.30 am, while small ginger is 08.45-11.00 am. Chromosome number of giant ginger and small ginger are 2n=2x=30, while red ginger is 2n=2x=22. Giant ginger has R= 3,109, Red ginger has R = 3,206 and small ginger has R = 4,065. Based on chromosome characters it is revealed that relationship between giant ginger and red ginger is closer that of compare to small ginger. This result is important as basic information for improving the gingers production through breeding program.Key words: Zingiber officinale Rosc., mitotic time, chromosome characterization, squashmethod
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/663
10.14499/indonesianjpharm23iss1pp54-59
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 54-59
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/663/529
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1462
2019-10-10T01:30:23Z
3:ART
Effect of Platelet-rich Plasma on Caspase-3 and IGF-1 mRNA expression in the diabetic rat testis
Istiqamah, Evi; Postgraduate Student of Master Biomedical Science, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Department of Midwifery, Faculty of Public Health, Universitas Muslim Indonesia Makassar, Indonesia
Rizal, Dicky Moch.; Department of Physiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Puspitasari, Ika; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
PRP, diabetic rat testis, caspase-3 mRNA expression, IGF-1, mRNA in expression
Testicular damage is a serious complication of diabetes mellitus resulting in male infertility, which is associated with caspase-3 and IGF-1 mRNA expression. Platelet-rich plasma (PRP), with its rich growth factor composition, has proven beneficial in regenerative therapy. It is believed that PRP has not been studied in testes for diabetes mellitus and there are no studies in the literature concerning the influence of PRP on expressions of growth factors in testes.The aim of this study was to investigate the efficacy of adjunctive PRP in insulin treatment for repair of testicular damage in a diabetic rat model. Diabetes was induced by administering single dose 60 mg/kg streptozotocin. Twenty Wistar male rats were divided into four groups: group 1, control group; group 2, diabetes without treatment; group 3, diabetes with treated insulin; and group 4, diabetes with treated insulin and PRP. Rats were euthanized after two weeks of treatment, and testes were taken for caspase-3 and IGF-1 mRNA expression measurements.Diabetes mellitus induction caused a significant increase in caspase-3 mRNA expression with p=0.049 and significant decrease in IGF-1 mRNA expression with p=0.004. There was no difference in caspase-3 and IGF-1 mRNA expression of the diabetic rat testis given insulin and PRP compared to without PRP.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-05-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1462
10.14499/indonesianjpharm30iss2pp98-104
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 98-104
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1462/902
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1101
2018-10-23T02:19:20Z
3:ART
Formulation and Evaluation of Glipizide Tablets Utilizing Hibiscus Rosasinensis Leaves Mucilage
Bahadur, Sanjib; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Roy, Amit; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Baghel, Pragya; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Choudhury, Ananta; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Saha, Suman; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Chanda, Ranabir; Bengal School of Technology, Sugandha, Delhi Road, Hooghly, West Bengal, 712102
This research work aims to develop glipizide tablets using Hibiscus rosasinensis leaves mucilage. The mucilage was extracted by using double distilled water and precipitated with ethanol. The precipitated mucilage was dried and grounded into powder. The tablet utilizing the mucilage as excipient was prepared by wet granulation method. The tablets were subjected to various tests. The evaluatory parameters of tablets were found to be within the limits as per United States Pharmacopoeia NF 24/19. FTIR spectrum reveals that there is no incompatibility between the ingredients used. Diabetes was induced in wistar albino rats using streptozotocin and effect of formulation on blood glucose level was determined. It was observed that the formulation could not sustain the release of glipizide. However, the glipizide release was retarded as the amount of mucilage was increased. It was observed that on the completion of antidiabetic study, the formulation could bring the blood glucose level to normal in group rats. However, the blood glucose level was still elevated in group administered with pure gliplizide. It can be concluded that HRM can be used to formulate glipizide tablets. The formulations with HRM shows better hypoglycemic activity and this may be attributed to antidiabetic activity of Hibiscus rosasinensis.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-03-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1101
10.14499/indonesianjpharm29iss1pp23
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 23
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1101/860
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/680
2020-01-27T02:53:16Z
3:ART
EXPRESSION OF RECOMBINANT HUMAN ERYTHROPOIETIN WITH GLYCOSYLATION MODIFICATION IN HEK293T CELLS
Septisetyani, Endah Puji; Research Center for Biotechnology, Indonesian Institute of Sciences Jl. Raya Bogor KM 46,
Cibinong, Bogor, 16911, Indonesia
Rubiyana, Yana; Research Center for Biotechnology, Indonesian Institute of Sciences Jl. Raya Bogor KM 46,
Cibinong, Bogor, 16911, Indonesia
Wisnuwardhani, Popi Hadi; Research Center for Biotechnology, Indonesian Institute of Sciences Jl. Raya Bogor KM 46,
Cibinong, Bogor, 16911, Indonesia
Wardiana, Andri; Research Center for Biotechnology, Indonesian Institute of Sciences Jl. Raya Bogor KM 46,
Cibinong, Bogor, 16911, Indonesia
Santoso, Adi; Research Center for Biotechnology, Indonesian Institute of Sciences Jl. Raya Bogor KM 46,
Cibinong, Bogor, 16911, Indonesia
Stability of erythropoietin (EPO) depends on its glycosylation states. With more glycosylation sites, the EPO protein will be more stable and also increase its half-life. A construct of recombinant human erythropoietin (rhEPO) which contains 2 additional N-link for glycosylation were designed. Based on translation analysis using ORF (open reading frame)-finder and protein alignment analysis using blast-p of NCBI home page, expected recombinant hEPO with additional 6-histidin tag in carboxyl terminus was expressed. HEK293T cells were transfected with recombinant plasmid containing rhEPO by using calcium phosphate method. Expression of rhEPO was detected by dot blot and Western blot analysis using hEPO antibody as the primary antibody and antirabbit antibody with alkaline phospatase linked as the secondary antibody. The bands were detected by BCIP/NBT color development substrate. The data indicated detection of EPO in culture medium of transfected HEK293T cells.Key words: HEK293T cell, calcium phosphate transfection, N-linked glycosylation, recombinant human erythropoietin
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/680
10.14499/indonesianjpharm23iss3pp177-182
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 177-182
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/680/546
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1564
2022-10-26T01:05:53Z
3:ART
Response Surface Methodology used in the Optimization of RP-HPLC Condition for Quantitative Analysis of Carmine and Rhodamine B
Nuvitasari, Reyna
Rohman, Abdul; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Martono, Sudibyo
The objective of this study was to optimize reversed-phase high-performance liquid chromatography (RP-HPLC) using an experimental design approach based on the response surface methodology of Central Composite Design (CCD) for separation and analysis of carmine (CAR) and Rhodamine B (RHO) in lipstick products. Some factors (independent variables) responsible for RP-HPLC separation including pH of buffer phosphate (X1), the acetonitrile ratio (X2), flow rate of mobile phase (X3), and column temperature (X4) were investigated. While, the responses (dependent variables) evaluated were resolution between CAR and RHO (Y1), tailing factor of CAR (Y2), tailing factor of RHO (Y3), retention time of CAR (Y4), retention time of RHO (Y5), peak area of CAR (Y6) and peak area of RHO (Y7). CCD showed that separation of CAR and RHO was influenced by these independent variables (factors). The optimum predicted conditions for the separation of CAR and RHO based on statistical results was pH buffer of 3.4, ACN 55%, the flow rate of 1.1 mL/min and column temperature of 35oC with the desirability of 1. Both CAR and RHO were clearly separated using optimum conditions, as suggested by CCD. The developed techniques were effective for optimizing chromatographic separation, therefore, the time consumption and a large number of running could be hindered.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-10-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1564
10.14499/indonesianjpharm30iss4pp276
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 276
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1564/922
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/441
2020-02-19T02:37:07Z
3:ART
PREFORMULATION OF DICLOFENAC SODIUM SUSTAINED RELEASE BY ION EXCHANGER RESINE
Zulkarnain, A. Karim; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
Yuwono, Tedjo; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
., Sumarno; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
The aim of the study was to find out a formula of granules giving a constant release of drug and also to search for the influence of pH on the drug release from the formula. One type of drug-resin complexes was used in this study ion exchanger the complex of diclofenac sodium and dowex. After 12 hours mixing the drug-resin complex was dried at 40 oC in an oven for 3 days. Afterwards, they were granulated and shieved. The particle size used were 20-40 mesh. The granules were tested for the dissolution of diclofenac sodium from the granules. The dissolution experiments were performed in a modified model of USP XX and the dissolution media used were phosphate buffer with the pHs of 5,8; 6,8 and 7.6 and the temperature was maintained at 37 oC. The amount of diug released in to the medium was assayed spectrophotometrically. The extent of dissolution was expressed as dissolution efficiency in 8 hours (DE8, % ). The results showed that the extent of dissolution of dtclofenac sodium from the granules complexed with the resin were low. The correlation between the amount of drug release from the complex and time was linier with the highest coefficient correlation having the ratio of l:5. The higher the pH values of the media, the higher the drug release from granules was. The drug release from drug-resin complex was well controlled which may be used for making a sustained release dosage form.Key Word : sustained release, diclofenac sodium, ion exchanger resin, granules
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/441
10.14499/indonesianjpharm0iss0pp20-27
Indonesian Journal of Pharmacy; Vol 12 No 1, 2001; 20-27
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/441/320
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1367
2019-10-03T03:16:50Z
3:ART
α-Glucosidase Inhibition Activity of Stem Bark Ceiba pentandra Linn. In Vitro and In Silico
Syihabudin, Vicri; Pakuan University
α-glucosidase; AutoDock Vina; LCMS/MS; vavain derivative
Ceiba pentandra (L.) stem bark plant contains tannin, flavonoid, alkaloid, terpenoid, and saponin compounds. It is known that flavonoid compounds have a potential for inhibition activity of an α-glucosidase enzyme. The purpose of this research is to know the α-glucosidase inhibition activity of the fraction of n-hexane, ethyl acetate, and water. These three fractions measured α-glucosidase inhibition activity by spectrophotometric UV-vis method. The fraction extract with IC50 values can best be done an analysis of active compounds using LCMS/MS methods, and results of the suspected compound do the binding molecule with a receptor model isomaltase α-glucosidase uses AutoDock Vina so that bond can predict drug active compounds. The results of this research activity are inhibition of α-glucosidase stem bark in the fraction of n-hexane 4,60 μg/mL, ethyl acetate fraction 8,55 μg/mL and fractions of the water 5,61 μg/mL. Analysis of the compound fraction of water by LCMS/MS eluted at the retention time 3,61 seconds allegedly derivative compounds (+) catechin and on retention time 8,70 minutes allegedly derivative compounds vavain. Vavain derivative compounds, (+) catechin, acarbose and quercetin each has its own docking score of -8,1; -8,8; -6,2 and -7,6 kcal/mol and has a semblance of bonding on amino acid Glu276 which is a residue catalytic role in hydrolysis reactions. Equality on the sides of active chemical bonds is expected to have the same activity as drug inhibition of the enzyme α-glucosidase.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Bina Lohita Sari, Novi Fajar Utami, Purnama Alam and Utari
2018-12-17
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1367
10.14499/indonesianjpharm29iss4pp206
Indonesian Journal of Pharmacy; Vol 29 No 4, 2018; 206
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1367/885
Copyright (c) 2018 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/514
2018-09-05T01:49:55Z
3:ART
EFFECTS OF AVURVEDIC SHODHANA (PROCESSING) ON DRIED TUBEROUS ACONITE (Aconitum napellus Linn.) ROOT
Paul, Arindam; Department of Pharmacology and Pharmacognosy, Babaria Institute of Pharmacy, BITS edu campus, Vadodara-Mumbai NH#8, Varnama, Vadodara - 391240, Gujarat, INDIA
Gajjar, Umang; Department of Pharmacology and Pharmacognosy, Babaria Institute of Pharmacy, BITS edu campus, Vadodara-Mumbai NH#8, Varnama, Vadodara - 391240, Gujarat, INDIA
Donga, Jignasa; Department of Pharmacology and Pharmacognosy, Babaria Institute of Pharmacy, BITS edu campus, Vadodara-Mumbai NH#8, Varnama, Vadodara - 391240, Gujarat, INDIA
Aconite (Aconitum napellus Linn.) commonly known as atis is a poisonous plant used extensively as antihypertensive, antipyretic, analgesic and antirheumatic. Ayurveda recommended the administration of aconite roots only after purification, i.e., boiling roots in cow’s urine (Gomutra). In the present study an attempt was made to compare the pro-arrhythmic and antihypertensive effects of powdered aconite root purified by shodhana process with that of unpurified form of aconite roots in order to provide scientific support of the claim in ayurvedic texts that purification of aconite root by shodhana process retains its antihypertensive activity and is devoid of pro-arrhythmic activity. Aconite root treatment in both forms purified and unpurified) caused significant reduction in BP when compared with diseased control group (P<0.05). The unpurified aconite root group showed significant increase in heart rate, increase in QRS complex time and increase in QT interval, however these parameters were statistically insignificant in purified aconite root treated group. The PRA, SC and BUN levels was significantly decreased in aconite root treatment groups. The probable mechanism of antihypertensive activity of aconite root can be attributed to decrease in plasma renin activity, decrease in oxidative stress and increase in NO levels. Key words: Aconite, shodhana process, antihypertensive, proarrhythmic
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2013-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/514
10.14499/indonesianjpharm24iss1pp40-46
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 40-46
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/514/392
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1521
2019-10-10T01:30:23Z
3:ART
The Effect of Thionamide to TRH, TSH, IL-4, T-REG, and Anti-TPO in Graves’ Disease
Decroli, Eva; Endocrinology and Metabolic Subdivision, Division of Internal Medicine, Dr. M. Djamil General Hospital/ Medical Faculty of Andalas University, Padang, West Sumatera, Indonesia
Elvira, Dwitya; Allergy and Immunology Subdivision, Division of Internal Medicine, Dr. M. Djamil General Hospital/ Medical Faculty of Andalas University, Padang, West Sumatera, Indonesia
Aprilia, Dinda; Endocrinology and Metabolic Subdivision, Division of Internal Medicine, Dr. M. Djamil General Hospital/ Medical Faculty of Andalas University, Padang, West Sumatera, Indonesia
TRH, TSH, T-reg, IL-4, anti-TPO
The most common cause of hyperthyroidism is Graves' disease. TRH and TSH are hormonal factors that modulate and control thyroid function in Graves' disease. In the immunological aspect, Graves' disease is played by the role of T-reg, IL-4, and anti-TPO. Graves' disease treatment goal is to inhibit thyroid hormone secretion by administering thionamide. The evaluation of this treatment is its hormonal and immunological aspects. To describe the effect of thionamide on serum TRH, TSH, IL-4, T-reg, and anti-TPO levels in Graves' disease. This study is a clinical trial study in 25 study participants. All study participants were given thionamide, namely PTU 300mg for three months and blood samples were taken for laboratory tests. Serum TRH, TSH, IL-4, T-reg FOXP3, and anti-TPO levels were examined by ELISA. The mean levels at the beginning and after three months of therapy are: serum TRH 92.589pg/mL and 115.944pg/mL; serum TSH 0.041mU/L and 0.223mU/L; serum IL-4 19.759pg/mL and 23.040pg/mL; T-reg FOXP3 gene polymorphism 0.621ng/mL and 0.518 ng/mL; serum anti-TPO 2697.539pg/mL and 2604.710pg/mL. Increased levels of serum TRH and TSH levels were statistically significant. The change in serum IL-4, T-reg FOXP3 gene polymorphism, and anti-TPO levels were not statistically significant. The administration of thionamide in Graves' disease for three months will significantly decrease Wayne index and serum FT4 levels, increase serum TRH and TSH levels.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-05-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1521
10.14499/indonesianjpharm30iss2pp122-127
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 122-127
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1521/897
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1104
2018-10-23T02:18:57Z
3:ART
Population-Based Approach to Analyze Sparse Sampling Data in Biopharmaceutics and Pharmacokinetics using Monolix and NONMEM
Nugroho, Akhmad Kharis; Department of Pharmaceutics Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta Indonesia
Hakim, Arief Rahman; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada
Hakim, Lukman; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada
model, population, sparse sampling data, Monolix, NONMEM
Although it has been developed since 1972, the implementation of a population-based modeling approach in Indonesia, particularly to analyze biopharmaceutics and pharmacokinetics data is still very limited. This study was aimed to evaluate the performance of Monolix and NONMEM, two of the popular software packages in a population-based modeling approach, to analyze the limited data (sparse sampling data) of the time profiles of the simulated plasma drug concentration of a theoretical compound. and NONMEM were used to model the limited data (40 data points) as a results of the random selection from the 180 point data of simulated plasma drug concentration (Cp) on 20 subjects at 0.25; 0.5; 0.75; 1; 1.5; 3; 6; 12 and 18 hours after per-oral administration of a 100mg of a theoretical compound. Population values of the absorption rate constant (Ka), the elimination rate constant (Kel) and volume of distribution (Vd) were compared to the average Ka, Kel and Vd obtained by the conventional method (two stage approach) using PKSolver on the Cp data of all subjects. The calculation system of a nonlinear mixed effect model in Monolix and NONMEM, successfully describes the sparse data, based on the visual evaluation of the goodness of fit. Comparison of parameter estimates of population values in Monolix and NONMEM are in the range of 94 to 108% of the real values of the rich data analysed by PKSolver. A population-based modeling can adequately analyze limited or sparse data, demonstrating its capability as an important tool in clinical studies, involving patients.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-12-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1104
10.14499/indonesianjpharm28iss4pp205
Indonesian Journal of Pharmacy; Vol 28 No 4, 2017; 205
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1104/853
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1176
2018-01-10T06:56:14Z
3:ART
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/674
2020-01-27T02:51:49Z
3:ART
IDENTIFICATION OF SAFETY ALERT BY MONITORING ANALYTICAL PARAMETERS AND HIGH-RISK DRUGS
Vilaplana, Vicente Escudero-; Pharmacy Department, Gregorio Marañón
University General Hospital Doctor Esquerdo 46, 28007 Madrid, Spain
Antúnez, María Gómez-; Internal Medicine Department, Gregorio
Marañón University General Hospital, Madrid, Spain
García, Esther Durán-; Pharmacy Department, Gregorio Marañón
University General Hospital Doctor Esquerdo 46, 28007 Madrid, Spain
Míguez, Antonio Muiño-; Internal Medicine Department, Gregorio
Marañón University General Hospital, Madrid, Spain
Sáez, María Sanjurjo-; Pharmacy Department, Gregorio Marañón
University General Hospital Doctor Esquerdo 46, 28007 Madrid, Spain
Early detection of adverse drug reactions (ADR) increases patient safety. Our objective was to identify ADR by monitoring laboratory parameters and high-risk drugs. We carried out a two-month prospective observational study in a Internal Medicine Department, with daily recording of drugs prescribed and the following parameters: Na, K, Ca, serum creatinine, glomerular filtration rate (GFR), INR, glucose, haemoglobin, platelets, ALT, AST, bilirubin, GGT, alkaline phosphatase, TSH, T4, and blood digoxin. High-risk drugs were closely monitored. 52 patients included, of whom 46.2% experienced an ADR. We observed an association with drugs in 25.5%, as follows: reduction in GFR, 26.9% (associated with loop diuretics [41.7%], angiotensin-converting enzyme [ACE] inhibitors [33.3%], angiotensin II receptor blockers [ARB] [16.6%], andanti-diabetic drugs [8.3%]); hypokalemia, 22.3% (associated with loop diuretics [50.0%], potassium-free fluid [37.5%], and salbutamol [12.5%]); hyperkalemia, 14.4% (associated with ACE inhibitors [60.0%] and ARB [40.0%]); INR out of range, 10.8% (associated with drug interactions [66.7%]); hyperglycemia, 8.1% (associated with corticosteroids [66.7%] and anti-diabetic drugs [33.3%]); and other conditions, 18.8%. We conclued that patient safety could be improved by implementing warnings in electronic prescriptions in cases of a decrease in GFR or modification of potassium levels in patients who are prescribed loop diuretics, ACE inhibitors, or ARBs.Key words: Adverse drug reaction, clinical decision support, high-risk drug, safety
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/674
10.14499/indonesianjpharm23iss2pp130-134
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 130-134
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/674/540
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1468
2019-10-28T01:20:02Z
3:ART
Antibacterial Compound from Aspergillus elegans SweF9 an Endophytic Fungus from Macroalgae Euchema sp.
Mulyani, Hani; 1. Pharmacy Faculty, Pancasila University,Jl. Raya Lenteng Agung Timur No.56-80, Srengseng Sawah, Jagakarsa, Jakarta
2. Indonesian Institute of Sciences (LIPI)
Dewi, Rizna Triana; Indonesian Institute of Sciences (LIPI)
Chaidir, Chaidir; Pharmacy Faculty, Pancasila University,Jl. Raya Lenteng Agung Timur No.56-80, Srengseng Sawah, Jagakarsa, Jakarta
Aspergillus elegans SweF9, antibacteria, (+)-epi-Epoformin.
The antibiotic resistance of bacterial pathogens has become a serious health concern and encouragement to search for novel and efficient antimicrobial metabolites. On the other hand, endophytic fungi have great potential as a natural source for antimicrobial agents. The objective of this study was to isolation antibacterial compound from endophytic fungi of A.elegans SweF9. The fungus was stationarily cultured at 30°C for 12 days in potato malt peptone (PMP) medium, then extracted with ethyl acetate. The antibacterial activities of the extract were evaluated by agar well diffusion method against Gram-poitive (Bacillus subtilis and Staphylococcus aureus) and Gram-negative (Escherichia coli) bacterial strains. The broth extract was able to inhibit the growth of E. coli, S. aureus and B. subtilis with antibacterial activity index compared to streptomycin sulfate were 84.6%, 91.6%, and 90% respectively. The active compound (1) was purified to yield amorphous white and identified using FTIR, NMR, and EI-MS analyses, revealed identified as (+) - epi-Epoformin. The compound showed an antibacterial activity index of E. coli, S. aureus and B. subtilis bacterial were 38%, 45%, and 47%, respectively. Based on these results endophytic fungi A. elegans SweF9 can be used as a new source of potential antibacterial compounds
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Indonesian Institute of Sciences
2019-09-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1468
10.14499/indonesianjpharm30iss3pp217
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 217
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1468/916
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/657
2020-01-27T02:53:58Z
3:ART
EFFECT OF BENZALDEHYDE EXCESS IN THE SYNTHESIS OF LR-2 AND CYTOTOXIC ACTIVITY OF LR-2 AGAINTS HeLa CELL
Ritmaleni, Ritmaleni; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip, Utara Yogyakarta 55281, Indonesia
Arifin, Muhammad Fajar; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip, Utara Yogyakarta 55281, Indonesia
Laksmiani, Ni Putu Linda; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Udayana, Bali 80221, Indonesia.
., Rumiyati; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip, Utara Yogyakarta 55281, Indonesia
., Sismindari; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip, Utara Yogyakarta 55281, Indonesia
LR-2(4-phenyl-3,4-dihydro-indeno[2’,1’]pyramidine-2(1H)- thione; Leni Ritmaleni 2), which designed and assumed to have biologically activity as anticancer, has been successfully synthesized by using the Biginelli reaction. This research was aimed to investigate the effect of benzaldehyde excess in the synthesis of LR-2 and to evaluate the cytotoxic activity of LR-2against HeLa cancer cell lines. The synthesis was done by reacting benzaldehyde, 2-indanone and together with thiourea at one time as said as one pot reaction synthetic methodology and the reaction was acid catalysed. The mole equivalent of benzaldehyde was in excess compare to others. The effect of benzaldehyde in excess is the higher the mole of benzaldehyde, the lower the yield of LR-2. The cytotoxicity of LR-2 was done by using MTT method and the LC50 was 268.15 μM.Key words : LR-2, benzaldehyde, cytotoxic, HeLa
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/657
10.14499/indonesianjpharm23iss1pp9-17
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 9-17
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/657/523
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1388
2019-10-03T03:16:50Z
3:ART
Screening of Antibacterial and Anticancer Activity of Soft Corals from Togean Islands, Indonesia
Zubair, Muhammad Sulaiman; Department of Pharmacy, Faculty of Sciences, Tadulako University, Kampus Bumi Tadulako, 94118, Palu, Indonesia
Lallo, Subehan; Faculty of Pharmacy, Hasanuddin University, 90245, Makassar, Indonesia
Rusmiyanti, Rusmiyanti; Department of Pharmacy, Faculty of Sciences, Tadulako University, Kampus Bumi Tadulako, 94118, Palu, Indonesia
Nugrahani, Arsa Wahyu; Department of Pharmacy, Faculty of Sciences, Tadulako University, Kampus Bumi Tadulako, 94118, Palu, Indonesia;
Jantan, Ibrahim; School of Pharmacy, Taylor’s University, Lakeside Campus, 1 Jalan Taylors, 47500 Subang Jaya, Selangor, Malaysia
Softcorals, Togean Islands, anti-cancer, antibacterial
Soft corals (Octocorallia, Alcyonaceae) have been reported to possess diverse biological activities and unique structural chemistry. This study aims to screen the potential antibacterial and anticancer activity of some soft corals collected from Togean Islands, Central Sulawesi, Indonesia. They were Lobophytum sp, Sarcophyton sp, Sinularia sp 1, and Sinularia sp 2. All dried coral materials were extracted for 3 x 24 h by maceration method using methanol and then evaporated by rotary evaporator to obtain viscous extracts. The determination of antibacterial activity had been performed by well agar diffusion method against Staphylococcus aureus and Escherichia coli. Meanwhile, the cytotoxic activity was performed by MTT method, followed by apoptosis annexin V-FTIC assay agains. Identification for the presence of terpenoids was performed by vacuum p-anisaldehyde-sulphuric acid spraying reagent on thin layer chromatography (TLC). Sinularia sp2 extract have strongly inhibited S. aureus and E.coli with the diameter of inhibition range from 12.76mm and 17.86mm, respectively. Moreover, Sinularia sp2 extract possessed also cytotoxic activity against human breast adenocarcinoma (MCF-7) and colorectal carcinoma (HCT-116) with the IC50 of 46.807 and 47.186 μg/mL, respectively. Extract Sinularia sp 1 was found to have strongest cytotoxicity on human colon colorectal carcinoma (HCT-116) with the IC50 of < 1.505 μg/mL. Annexin V-FTIC assay clearly exhibited that the apoptosis mechanism is proposed by the extracts of Sinularia sp1 and Sinularia sp 2. Terpenoids were identified on both extracts suggesting for further purification and isolation for the bioactive terpenoid compounds.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-11-21
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1388
10.14499/indonesianjpharm29iss4pp173
Indonesian Journal of Pharmacy; Vol 29 No 4, 2018; 173
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1388/880
Copyright (c) 2018 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/691
2020-01-27T02:53:31Z
3:ART
SYNTHESIS AND ANALGESIC ACTIVITY OF 6-(MNITROPHENYL)-4-SUSTITUTED BENZYLIDENE-4,5-DIHYDROPYRIDAZIN-3(2H)-ONE DERIVATIVES
Asif, Mohammad; Department of Pharmacy, GRD (P.G) Institute of Management and Technology, Dehradun,
248009, India.
Many research groups have been interested in 3(2H)-pyridazinones for the development of potential analgesic and antiinflammatory agents. Stimulated by these findings, three 6-(mNitrophenyl)-4-sustituted benzylidene-4,5-dihydropyridazin-3(2H)-ones (IVa-IVc) have been synthesized and evaluated for analgesic activity against hot plate method. Compounds IVa-IVc were prepared from 6-m-nitrophenyl-4,5-pyridazin-3(2H)-one by condensation with respective aldehydes. All compounds (IVa-IVc) having nitro phenyl and benzylidene groups at position 6 and 4 of the pyridazinone ring receptively. All compounds (IVa-IVc) showed significant analgesic activity when compare to control group and were found less potent than reference drug aspirin.Key words:Pyridazinones, analgesic, benzylidene, nitrophenyl.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/691
10.14499/indonesianjpharm23iss4pp254-258
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 254-258
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/691/557
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1601
2021-01-07T01:00:39Z
3:ART
FTIR Method for Peptide Content Estimation and Degradation Kinetic Study of Canarium Nut Protein
Oktaviary, Rozana
Nugrahani, Ilma
Surantaatmadja, Slamet Ibrahim
Kartawinata, Tutus Gusdinar
Apsari, Cintya Nurul
Canarium nut; peptide; degradation; FTIR; kinetic
Quantitative analysis of bioactive peptide mostly conducted by measuring the activity. While the determination of peptide content in natural sources has been conducted using various instruments, vibrational spectroscopy remains underutilized. Here, we attempted new developed method in peptide quantification and degradation kinetic analysis using Fourier-Transform Infrared Spectroscopy. Bovine Serum Albumin was used as standard protein in method development and validation. Peptide content was estimated by converting peak area to concentration. The method was used to estimate peptide content in Canarium nut protein and its hydrolysates, which potentially hold biological activity. Kinetic study was conducted with microwave as an accelerator for hydrolysis, an apparatus rarely used in peptide study. Amide I band on wavenumber range of 1724.05-1619.91 cm-1 was selected for analysis, considering its selectivity and linearity. The method also met other validation requirement, including accuracy and precision. When applied in quantitative analysis, the method was able to calculate peptide content decrease in Canarium nut protein after hydrolysis using papain (38.24%), pepsin (33.67%) and alkaline reagent (28.53%). In kinetic study, microwave-assisted peptide degradation exhibited logarithmic profile with the equation of y=-0.148ln(x)+0.9591 and R² value of 0.963. Based on these results, FTIR is useful in estimating peptide content and in analyzing degradation kinetic profile.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-07-10
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1601
10.14499/indonesianjpharm31iss2pp78
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 78
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1601/949
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/509
2018-09-05T01:49:54Z
3:ART
DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHY AND SPECTROSCOPIC METHODS FOR THE ANALYSIS OF DOXOFYLLINE IN PHARMACEUTICAL DOSAGE FORMS
Thiruvengadam, Ethiraj; Dept of Pharmaceutics &Pharmaceutical Analysis. The Erode College of Pharmacy, Erode, Tamilnadu, 638112, India
Ramadoss, Revathi; Dept of Pharmaceutics &Pharmaceutical Analysis. The Erode College of Pharmacy, Erode, Tamilnadu, 638112, India
Vellaichamy, Ganesan; Dept of Pharmaceutics. The Erode College of Pharmacy, Erode, Tamilnadu, 638112, India
A high performance liquid chromatography (HPLC) and ultraviolet spectroscopic (UV) methods were developed and validated for the quantitative estimation of doxofylline (DF) in pharmaceutical dosage forms. HPLC was carried out using reversephase technique on RP-8 column with a mobile phase composed of 0.05M phosphate buffer pH 6 and acetonitrile (60:40, v/v). The mobile phase was pumped at a flow rate of 1mL/min, and detection was made at 230nm with PDA detector. UV method was performed with λ max at 270nm with apparent molar absorptive of 0.878x103 L mol-1 cm-1. Both the methods showed good linearity, recovery and precision. No spectral or chromatographic interferences from the tablet excipients were found in UV and HPLC methods. The various parameters such as linearity, precision, accuracy, specificity, and robustness, limit of detection and limit of quantization were studied according to ICH guidelines. Statistical analysis was done by student’s t-test and F-test, which showed no ignificant difference between the results of both methods. So the proposed methods could be applicable for routine analysis of DF and monitoring of the quality of marketed drugs. Key words: Doxofylline, Validation, HPLC, UV spectroscopy, Comparisonstudies, Student’s t-test, F-test.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2013-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/509
10.14499/indonesianjpharm24iss1pp14-21
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 14-21
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/509/387
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1505
2019-10-10T01:29:33Z
3:ART
In Vitro Study of the Combination of Doxorubicin, Curcuma xanthorrhiza, Brucea javanica, and Ficus septica as a Potential Novel Therapy for Metastatic Breast Cancer
Sutejo, Ika Rahmawati; Biochemistry Department, Faculty of Medicine, University of Jember, Jalan Kalimantan 37 Jember, Indonesia
Cancer Chemoprevention Research Center, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Putri, Herwandhani; Cancer Chemoprevention Research Center, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Handayani, Sri; Cancer Chemoprevention Research Center, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Research Center for Chemistry, Indonesian Institute of Sciences (LIPI), Gedung 452 Kawasan PUSPIPTEK Serpong, Tangerang Selatan, Banten 15314, Indonesia
Jenie, Riris Istighfari; Department of Pharmaceutical Chemistry Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Cancer Chemoprevention Research Center, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Meiyanto, Edy; Professor of Pharmaceutical chemistry, Faculty of Pharmacy. Universitas Gadjah Mada
C xanthorrhiza, Bjavanica, F septica, antimetastasis, 4T1 cells
Less optimized therapeutic effects constrain the use of doxorubicin as the main agent of chemotherapy for metastatic breast cancer, resistance and side effects. Therefore we need a combination of more than one chemopreventive agent which has different molecular targets to solve that problem. The aims of this study is to prove the inhibitory effect of ethanolic extract of rhizome of Curcuma xanthorrhiza (ECx), fruit of Brucea javanica (EBj), leave of Ficus septica (EFs) and doxorubicin (Dox) alone and its combination on migration and invasion of a highly metastatic 4T1 breast cancer cell line. Cytotoxic activity of single and combination treatment was evaluated by MTT assay, followed by an experiment of apoptosis induction by using flow cytometry. The inhibitory effect on migration was observed by the scratch wound-healing assay. Furthermore, the observation of the activity of matrix metalloproteinase-9 (MMP-9) was analyzed by gelatin zymography. The results showed that ECx, EBj, EFs, and Dox has cytotoxic activity on 4T1 cells with the value of IC50 respectively 49.7±1.53mg/mL, 59.9±1.79mg/mL, 15.2±2.12mg/mL and 1.2±0.23mM. Furthermore, combination of ECx-EBj-Dox and ECx-EBj-EFs revealed synergistic effect on 4T1 cells and decrease cell viability through the induction of apoptosis and necrosis. Based on wound healing assay, 24 hours incubation of this combination inhibited 4T1 cells migration compared to single treatment. Gelatin zymography analysis showed that this combination also inhibited the activity of MMP-9 greater than a single use. Curcuma xanthorrhiza, Brucea javanica, and Ficus septica may have potential to be developed as a combination with or without doxorubicin for metastatic breast cancer treatment.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1505
10.14499/indonesianjpharm30iss1pp15
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 15
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1505/889
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1130
2018-01-09T08:39:19Z
3:ART
Evaluation of In vitro and In vivo Antioxidant potential of Morinda reticulata Gamble Tubers in Wistar Albino Rats Subjected to CCl4 and Paracetamol induced Hepatotoxicity
Asirvatham, Raju
Usha, Joshila Jose
M.reticulata; in vitro and in vivo antioxidant; DPPH; Sylimarin; Melondyaldehyde (MDA); Reduced Glutathione (GSH); Super oxide dismutase (SOD)
The aim of present study is to explore the antioxidant potential of ethanol and aqueous extracts of Morinda reticulata Gamble by in vitro and in vivo methods. In vitro antioxidant activity of Benzene, Chloroform, Ethanol and aqueous extracts of M. reticulata was studied by DPPH, Metal chelating, Super oxide free radical scavenging assay, Hydroxyl radical scavenging assay and Reducing power assay where ascorbic acid was used as a standard antioxidant. Oxidative stress in Wistar rats was induced by two different models using administration of CCl4 (1.5 ml/kg, p.o) and paracetamol (2g/kg) on seventh day of study. Ethanol and aqueous extracts were given twice daily for one week. Silymarin (25 mg/kg, p.o) was given as a standard drug. In in vitro, IC50 values were least with ethanol and aqueous extracts when compared with other extracts. Ethanol extracts exhibited similar free radical scavenging effect as that of standard ascorbic acid. In vivo antioxidant activity was assessed by the measurement of Melondyaldehyde (MDA), Reduced Glutathione (GSH), Super oxide dismutase (SOD) and Total protein levels were estimated from the liver tissue homogenate. The level of MDA (3.27±2.18) was significantly (p˂0.001) increased whereas decreased level of total protein, GSH and SOD were found in CCl4 and paracetamol control group. Seven days extracts treatments restored these altered parameters in to normal where ethanol extract treatment group showed significant (p˂0.001) result than aqueous extract. HPTLC study showed that presence of six phytoconstituents with corresponding Rf value. The study report concluded that M.reticulata has very good antioxidant effect against CCl4 and paracetamol induced liver damage with oxidative stress. Probable mechanism behind this protection against oxidative damage produced by CCl4 and paracetamol is its phytoconstiuents.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-08-08
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1130
10.14499/indonesianjpharm28iss3pp147
Indonesian Journal of Pharmacy; Vol 28 No 3, 2017; 147
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1130/832
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1130/108
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1493
2021-01-07T01:00:39Z
3:ART
THE EFFECT OF GIVING DELTAMETHRIN AND CYPERMETHRIN INSECTICIDES ON THE LEVEL OF INTRATESTICULAR TESTOSTERONE IN MALE WISTAR STRAIN RATS
Triutomo, Devyanto Hadi; Faculty of Pharmacy UGM
Puspitasari, Ika; Faculty of Pharmacy, Universitas Gadjah Mada
Susidarti, Ratna Asmah; Faculty of Pharmacy, UGM
cypermethrin, deltamethrin, intratesticular testosterone
The use of deltamethrin and cypermethrin insecticides to control pests has been implemented in Indonesia. Irregular and excessive use of insecticides can have toxic effects on the male reproduction system. Deltamethrin and cypermethrin insecticides can inhibit androgen biosynthesis and disrupt the growth of sperm. Therefore, this research is conducted to find out the effect of deltamethrin and cypermethrin insecticides toward the level of intratesticular testosterone in male Wistar strain rats. This research is conducted in vivo using male Wistar strain rats. Nine rats are divided into three groups of treatment, namely control group, deltamethrin group with 0.26 mg/kg of weight, and cypermethrin group with 0.26 mg/kg of weight. Treatment is conducted every day for 15 days orally, and then the rats are dissected to take their testicles. Testicles are chopped and their intratesticular homogenates are taken by adding the medium of DMEM and collagenase (0.25 mg/mL). The measurement of the level of testosterone is conducted by using Electrochemiluminescence immunoassay (ECLIA) method. Data on the change of the increase of body weight, organ weight, and the level of testosterone is analyzed statistically using the one-way ANOVA test. The research result shows that giving 0.26 mg/kg of weight of deltamethrin and 0.26 mg/kg of weight of cypermethrin does not affect the increase in body weight, relative weight of the reproductive organ, and the level of intratesticular testosterone of male Wistar strain rats.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-08-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1493
10.14499/indonesianjpharm31iss2pp116
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 116
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1493/947
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/669
2020-01-27T02:51:49Z
3:ART
DIFFERENTIATION BETWEEN PORCINE AND BOVINE GELATIN IN CAPSULE SHELLS BASED ON AMINO ACID PROFILES AND PRINCIPAL COMPONENT ANALYSIS
Widyaninggar, Amalia; Research Center of Halal Products, Universitas Gadjah Mada, Yogyakarta 55281,
Indonesia
., Triwahyudi; Research Center of Halal Products, Universitas Gadjah Mada, Yogyakarta 55281,
Indonesia
Triyana, Kuwat; Research Center of Halal Products, Universitas Gadjah Mada, Yogyakarta 55281,
Indonesia
Rohman, Abdul; Research Center of Halal Products, Universitas Gadjah Mada
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
Gelatin used for capsule shells can be derived from porcine origin. Some religion like Islam prohibited their followers to consume any products containing porcine derivatives including porcine gelatin in the products. Consequently, some rapid and reliable techniques are continuously developed for detection of porcine gelatin in some pharmaceutical products like capsule shells. The aim of this study was to evaluate the possibility to use amino acid profile in combination with chemometrics of principal component analysis for detection and classification between gelatin in capsule derived from porcine and bovine. Based on score plot of first principal components (PC1) and second principal components (PC2), porcine and bovine gelatins in capsule could be apparently distinguished.Key words: bovine gelatin; porcine gelatin; amino acid profile; principal component analysis
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/669
10.14499/indonesianjpharm23iss2pp104-109
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 104-109
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/669/535
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1519
2019-10-28T01:20:02Z
3:ART
A Study of Psychoactive Medicines and Risk of Falls Among Indonesian Elderly Patients
Rahmawati, Fita; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia 55281
Mustafidah, Nasikhatul; Postgraduate Magister of Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia 55281
Department of Pharmacy RSUD. Dr. Soedono Madiun
Pramantara, I Dewa Putu; Department of Geriatric, RSUP Dr. Sardjito Hospital, Yogyakarta, Indonesia 55281
Wahab, Izyan Abdul; Faculty of Pharmacy, Cyberjaya University College of Medical Sciences, Persiaran Bestari, Cyber 11, 63000 Cyberjaya, Selangor, Malaysia
Psychoactive medicine, Fall risk, Elderly
One of the causes of injury to the elderly is due to falls. Falling can be prevented by identifying and controlling risk factors. One risk factor that can be controlled is the use of fall risk medicines including psychoactive. This study aims to identify the association between the use of psychoactive medicine and its characteristic with the risk of falls among the elderly in Indonesia. The study utilized a case-control study design for a total number of 414 elderly patients, during October until December 2018. Cases were elderly aged 60 years or above with a high risk of falling assessed using the Morse Fall Scale (MFS≥45). Each case was matched with up to two randomly selected controls of the same age who are classified as low to moderate risk of falling (MFS<45). The use of psychoactive medicines was screened from a history of drug use for the past six months. Psychoactive medicine-fall risk associations were estimated via logistic regression. There were 138 cases and 276 controls. The median age of subjects was 66 years old and 54.83% was a woman. Elderly with a high risk of falling had higher psychoactive medicines use when compare with controls (31.16 % vs 21.38 %, p< 0.05). After adjusting for potential confounders, the use of psychoactive medicines was significantly associated with higher fall risk in elderly patients (OR 1.79 95% CI 1.10-2.90). Only the duration of psychoactive medication use over 90 days was significantly associated with a high risk of falling (AOR 3.65 95% CI 1.46-9.14). In elderly patients, the continued use of psychoactive medicines increased the risk of fall. Prescribers need to weigh risk and benefit from the use of psychoactive medicines in the elderly to prevent future fall.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Faculty of Pharmacy Universitas Gadjah Mada
2019-07-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1519
10.14499/indonesianjpharm30iss3pp233
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 233
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1519/907
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1329
2018-10-23T02:19:33Z
3:ART
Effects of Probiotics and Vitamin B Supplementation on IFN-γ and IL-12 Levels During Intensive Phase Treatment of Tuberculosis
Suprapti, Budi; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Suharjono, Suharjono; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Raising, Rahmawati; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Yulistiani, Yulistiani; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Izzah, Zamrotul; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Nilamsari, Wenny Putri; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Wulaningrum, Prastuti Asta; Department of Pulmonology and Respiratory Medicine, Universitas Airlangga Teaching Hospital, Surabaya
Bachtiar, Arief; Department of Pulmonology and Respiratory Medicine, Universitas Airlangga Teaching Hospital, Surabaya
Interleukin-12, Interferon-γ, Probiotics, Tuberculosis, Vitamin B
Tuberculosis is an acute infectious disease that primarily affects the lungs. Probiotics supplementation can increase the number and activity of NK cell in peripheral blood by modulation of IL-12, thus increasing IFN-γ production by Th1 response. Vitamin B1 acts on macrophages and affects neutrophil motility. Vitamin B6 is associated with the release of cytokines and the responsiveness of NK cells, while vitamin B12 affects to lymphocytes, Tcell proliferation, CD4+ ratios, and NK cell activity. To analyze the effects of probiotics and vitamin B1, B6, B12 supplementation on IFN-γ and IL-12 levels during intensive phase of antituberculosis treatment. The study was pre-post test randomised control by time series. The control group was TB patients with standard therapy of antituberculosis and vitamin B6, while the intervention group was TB patients receiving therapy plus once daily probiotics and vitamin B1, B6, B12supplementation during the intensive phase. Blood samples were withdrawn at baseline, one month, and two months after therapy to measure plasma IFN-γ and IL-12 levels using the ELISA method. Twenty two patients were divided equally into two groups. There was a tendency to greater increase of IFN-γ in the first month of the intervention group, followed by a significant decline after two-month therapy (p < 0.05). In both groups there was a rise in IL-12 levels after one month followed by a decrease in the second month (p > 0.05). However, the percentage was higher in the supplementation group. Adding probiotics and vitamins B1, B6, B12 could improve immune response through IL-12 and IFN-γ modulation during intensive phase therapy.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-06-22
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1329
10.14499/indonesianjpharm29iss2pp80
Indonesian Journal of Pharmacy; Vol 29 No 2, 2018; 80
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1329/868
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1310
2018-11-02T06:46:53Z
3:ART
Derivatives of 3-(alkylthio)-5-(thiophen-2-ylmethyl)-4H-1,2,4-triazol-4-amines as increasing efficiency substances
Safonov, Andrey; Zaporizhzhia State Medical Univercity
1,2,4-triazole, pharmacological correction of fatigue, method of forced swimming
In today's society, especially in eastern countries such as China, Japan etc., the problem of fatigue and even death at work is acute. One of the solution to this problem is complex therapy with increasing efficiency drugs. Derivatives of 1,2,4-triazole have already proven themselves as potential compounds for pharmacological correction of fatigue. Compounds were synthesized at the Department of toxicological and inorganic chemistry ZSMU. Using method of forced swimming on a group of white nonlinear rats activity of anti-fatigue of the compounds were analyzed. Having analyzed the data of pharmacological correction of fatigue for 3-(alkylthio)-5-(thiophen-2-ylmethyl)-4H-1,2,4-triazol-4-amines, it should be noted that this series of compound do not show anti-fatigue effect. Introduction in molecule 3- (nonylthio)-5-(thiophen-2-ylmethyl)-4H- 1, 2, 4-triazol-4-amine 4-fluorbenzylidene, 4-(dimethylamino) benzylidene, 4- methoxybenzylidene, 2-chloro-6-fluorobenzylidene radicals lead to increase actoprotective effect. The most active compound is N-(2-chloro-6-fluorobenzylidene)-3-(nonylthio)-5-(thiophen- 2- ylmethyl)- 4H- 1, 2, 4- triazol- 4-amine which exceeds the standard (riboxin).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Zaporizhzhia State Medical University
2018-09-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1310
10.14499/indonesianjpharm29iss3pp167
Indonesian Journal of Pharmacy; Vol 29 No 3, 2018; 167
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1310/878
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/686
2020-01-27T02:53:31Z
3:ART
CHOCOLATE FORMULATION AS DRUG DELIVERY SYSTEM FOR PEDIATRICS
Mayank, Sharma; College of Pharmacy, IPS Academy, Rajendra Nagar, A.B. Road, Indore - 452012 (M.P.), India
Kumar, Jain Dinesh; College of Pharmacy, IPS Academy, Rajendra Nagar, A.B. Road, Indore - 452012 (M.P.), India
Chocolate is highly sophisticated and infinitely a versatile food that can be combined to create completely different taste and texture sensations. The objective of the present study is to develop a palatable chocolate formulation of Domperidone and Cetirizine for pediatric administration and to increase patient’s desire to consume the medication. In present investigation chocolate base is prepared by use of cocoa powder, coco butter, lecithin, and pharmaceutical grade sugar. Thereafter drug is incorporated to prepared chocolate base. The medicated chocolate prepared is evaluated for appearance, moisture content determination using digital Karl Fischer titrator, blooming test, drug content determination & drug excipient interaction by differential scanning calorimetry.Key words: Medicated Chocolate, Pediatrics, Chocolate formulation
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/686
10.14499/indonesianjpharm23iss4pp216-224
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 216-224
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/686/552
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1410
2022-10-26T01:05:53Z
3:ART
In Vitro Study: Effect of Cobalt(II) Chloride Against Dengue Virus Type 1 in Vero Cells
Sucipto, Teguh Hari; Institute of Tropical Disease, Universitas Airlangga
Untoro, Yovilianda Maulitiva; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga
Setyawati, Harsasi; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga
Maharani, Anisa; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga
Sholihah, Novia Faridatus; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga
Churrotin, Siti; Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga
Amarullah, Ilham Harlan; Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga
Soegijanto, Soegeng; Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga
Cobalt(II) chloride, DENV-1, and Antiviral activity.
Dengue virus (DENV) serotypes DENV-1 to DENV-4 are enveloped viruses that belong to the genus Flavivirus of the Flaviviridae. Dengue vaccine or antiviral has not yet been clinically approved for humans, even though there have been great efforts toward this end. Antiviral activity against DENV is needed to develop to be an alternative drug for DENV virus. Cobalt(II) chloride have been used in the treatment and prevention of diseases of humans since ancient times. The aim of this study is to investigate the antiviral effects and Cytotoxicity of Cobalt(II) chloride. This compound was further investigated for its inhibitory effect on the replication of DENV-1 in Vero cells. Antiviral activity and Cytotoxicity measured by WST-1 assay. The IC50 value of the Cobalt(II) chloride for DENV-1 was 0.38 μg/ml. The cytotoxicity of Cobalt(II) chloride to Vero cell suggest that the CC50 value was 2.91 µg/ml The results of this study demonstrate the anti-dengue serotype 1 inhibitory activity of Cobalt(II) chloride was a high toxic compound.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-01-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1410
10.14499/indonesianjpharm30iss4pp316
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 316
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1410/931
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1273
2018-10-23T02:18:57Z
3:ART
Review of Insulin Therapy In Type 2 Diabetes Mellitus Ambulatory Patients
Suprapti, Budi; Faculty of Pharmacy Universitas Airlangga, Jalan Dharmawangsa Dalam, Surabaya, Indonesia
Widyasari, Nia; Faculty of Pharmacy Universitas Airlangga, Jalan Dharmawangsa Dalam, Surabaya, Indonesia
Rahmadi, Mahardian; Faculty of Pharmacy Universitas Airlangga, Jalan Dharmawangsa Dalam, Surabaya, Indonesia
Wibisono, Cahyo; Faculty of Medicine Universitas Airlangga, Jalan Dr. Moestopo, Surabaya, Indoensia
Diabetes mellitus, insulin, ambulatory patient, DUR, DRP
The purposes of this study were to review utilization of insulin therapy in type 2 diabetes mellitus out patients and identify its drug related problems. The data were collected cross-sectionally with purposive sampling method in the period March 2016 until May 2016 in Outpatient Clinic Universitas Airlangga Teaching Hospital Surabaya. The results of 240 patients showed that insulin was used as monotherapy insulin in 2,9% patients; combination 1 insulin & 1-4 OAD in 31,3%; basal bolus therapy 27,9%; combination basal−bolus therapy & 1-3 OAD 43,9%. Based on blood glucose target achievement, only 20,8% of patients achieve the target, 75,1% failed to achieve the target and 4,1% suffered from hypoglycemia. Drug related problems identified adverse drug reaction of antidiabetic therapy such as hypoglycemia (6.7%), nausea (3.8%), bloating (1.3%), increase of flatulency (2.9%) and inappropriate combination (0,4%). In conclusion insulin therapy was complicated and individually, most of the patients still did not reach the target and there was potential drug related problem in this patients group. So that caring from solid inter-professional health collaboration is needed
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Faculty of Pharmacy Universitas Airlangga, Jalan Dharmawangsa Dalam, Surabaya, Indonesia
2017-12-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1273
10.14499/indonesianjpharm28iss4pp221
Indonesian Journal of Pharmacy; Vol 28 No 4, 2017; 221
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1273/855
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1820
2021-01-06T13:13:53Z
3:ART
The effect of combination of active fraction Andrographis paniculata (Burm.f) Ness and Centella asiatica (i) Urban on the alpha glucocidase inhibitor and antioxidant activities
Rusman, Arman; Master program, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
Nugroho, Agung Endro; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281
Pramono, Suwidjiyo; Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281
The Andrographis panicullata and Centella asistica extract have been reported that had a anti-diabetic effect. However, the specific mechanism and the effect combination of both were not yet reported. This study was purposed to determine the potency of extract, fractions and the combination of Andrographis panicullata (AP) and Centella asistica (CA) active fraction to inhibit alpha glucosidase enzymeand its ability to reduce DPPH radical.AP and CA were extracted using 50% ethanol then fractionated with solvents under different polarity levels. The inhibiting activity to alpha glucosidase enzyme and antioxidant activity of each fractions was tested. The most active fractions from AP and CA were then combined and re-tested for activity. The results result reported that both of AP and CA had inhibition of alpha glucosidase activity and antioxidant activity. Based on calculation combination index (CI) of of active fraction of AP and CA showing in alpha glucocidase activity had a antagonist action and antioxidant had a sinergic action. Therefore, combination of AP and CA not has not recomended for alpha glucocidase inhibitor but the combination has ability to reduce DPPH radical.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-04-09
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1820
10.14499/indonesianjpharm31iss1pp56
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 56
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1820/945
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1820/297
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/664
2020-01-27T02:53:58Z
3:ART
PHYTOCHEMICAL SCREENING AND ANALYSIS POLYPHENOLIC ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF WHITE DRAGON FRUIT (Hylocereus undatus)
VH, Elfi Susanti; Department of Chemistry, PMIPA FKIP, Universitas Sebelas Maret. Jl. Ir Sutami
36 A Surakarta Indonesia, 57126
Utomo, Suryadi Budi; Department of Chemistry, PMIPA FKIP, Universitas Sebelas Maret. Jl. Ir Sutami
36 A Surakarta Indonesia, 57126
Syukri, Yandi; Departement of Pharmacy, FMIPA, Universitas Islam Indonesia Jl. Kaliurang KM 14,5 Sleman Yogyakarta 55584
Redjeki, Tri; Departement of Pharmacy, FMIPA, Universitas Islam Indonesia Jl. Kaliurang KM 14,5 Sleman Yogyakarta 55584
White dragon fruit is a well known and widely used herbal medicine, especially in Asia, which contains several interesting bioactive constituents and possesses health promoting properties. The aim of this study was to analyze for the bioactive compounds, evaluate total phenolic contents and antioxidant capacities of methanolic extract of white dragon fruit. The antioxidant activity was determined by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay. Total phenolic content were determined by Folin-Ciocalteu method. Phytochemical screening of the white dragon fruit showed the presence of triterpenoid, alkaloid, flavonoid and saponin. The extract exhibited strong antioxidant activity with IC50 of 193 μg/mL, and total phenolic content of 246 μg/mL in 1 Kg dry extract.Key words: antioxidant activity, total phenolic, DPPH, white dragon fruit
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/664
10.14499/indonesianjpharm23iss1pp60-64
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 60-64
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/664/530
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1495
2019-10-10T01:30:23Z
3:ART
Evaluation of Pain Scale Decrease and Adverse Effects of Ketorolac Injections: An Observational Study in Patients with Postoperative Pain
Ihsan, Mawardi; Department of Pharmacology and Clinical Pharmacy, Universitas Gadjah Mada
Kurniawati, Fivy; Department of Pharmacology and Clinical Pharmacy, Universitas Gadjah Mada
Khoirunnisa, Husna; Undergraduate Program of Pharmacy, Universitas Gadjah Mada
Chairini, Belladonna; Undergraduate Program of Pharmacy, Universitas Gadjah Mada
The use of ketorolac injections in Indonesia is restricted with the provision of 2-3 ampoules per day with a maximum of two days even though the literature states that ketorolac could be used for no more than five days. This study aimed to determine the decrease in pain scale as well as gastrointestinal and renal adverse effects of ketorolac injections in two days of use. This study was an observational study with one-group pre-test post-test design conducted prospectively. The group was a group of patients with postoperative pain who received ketorolac injections and were treated during January till April 2018 in an academic hospital in Yogyakarta. The results showed that ketorolac injections did not provide a statistically significant decrease in pain scale in two days of use compared to before surgery (median [range] = 2.0[0.0-9.33] vs 1.33[0.0-8.33]; p=0.32). Ketorolac injections decreased the kidney function of subjects in two days of use compared to before surgery based on creatinine values (0.76mg/dL vs 0.80mg/dL; p=0.024) and GFR (96.13mL/min/m2 vs 87.52mL/min/m2; p=0.023), and as many as 31 subjects (43.06%) experienced complaints that were suspected to be the gastrointestinal adverse effects of ketorolac injections with the three most complaints were bloating (18.06%), nausea (16.67%), and heartburn (15.28%). Those three results support the use of ketorolac injections following what has been regulated in the Indonesian National Formulary.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-05-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1495
10.14499/indonesianjpharm30iss2pp133-140
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 133-140
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1495/903
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1289
2018-10-23T02:19:20Z
3:ART
In Vitro Evaluation of Anti-Inflammatory and Anti-Diabetic Potential Effects of Euphorbia Tithymaloides Ethanol Extract
Wandita, Theresia Galuh; Departement of Animal Life and Environmental Science, HANKYONG NATIONAL UNIVERSITY, SOUTH KOREA
Joshi, Najuma; Departement of Animal Life and Environmental Science, HANKYONG NATIONAL UNIVERSITY, SOUTH KOREA
dela Cruz, Joseph Flores; Department of Basic Veterinary Sciences
College of Veterinary Medicine
University of the Philippines Los Banos, PHILIPPINES
Hwang, Seong Gu; Departement of Animal Life and Environmental Science, HANKYONG NATIONAL UNIVERSITY, SOUTH KOREA
Hibiscus rosasinensis, glipizide, mucilage, antidiabetic activity, excipients
This research work aims to develop glipizide tablets using Hibiscus rosasinensis leaves mucilage. The mucilage was extracted by using double distilled water and precipitated with ethanol. The precipitated mucilage was dried and grounded into powder. The tablet utilizing the mucilage as excipient was prepared by wet granulation method. The tablets were subjected to various tests. The evaluatory parameters of tablets were found to be within the limits as per United States Pharmacopoeia NF 24/19. FTIR spectrum reveals that there is no incompatibility between the ingredients used. Diabetes was induced in wistar albino rats using streptozotocin and effect of formulation on blood glucose level was determined. It was observed that the formulation could not sustain the release of glipizide. However, the glipizide release was retarded as the amount of mucilage was increased. It was observed that on the completion of antidiabetic study, the formulation could bring the blood glucose level to normal in group rats. However, the blood glucose level was still elevated in group administered with pure gliplizide. It can be concluded that HRM can be used to formulate glipizide tablets. The formulations with HRM shows better hypoglycemic activity and this may be attributed to antidiabetic activity of Hibiscus rosasinensis.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-03-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1289
10.14499/indonesianjpharm29iss1pp1
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 1
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1289/858
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/681
2020-01-27T02:53:16Z
3:ART
A BIOAVAILABILITY STUDY OF INDONESIAN GENERIC TABLET OF CAPTOPRIL IN HEALTHY VOLUNTEERS
Nugroho, Agung Endro; Laboratory of Pharmacology and Toxicology, Department of Pharmacology and Clinical
Pharmacy, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Jogjakarta, Indonesia, 55281
Hakim, Arief Rahman; Laboratory of Pharmacology and Toxicology, Department of Pharmacology and Clinical
Pharmacy, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Jogjakarta, Indonesia, 55281
., Purwantiningsih; Laboratory of Pharmacology and Toxicology, Department of Pharmacology and Clinical
Pharmacy, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Jogjakarta, Indonesia, 55281
Hakim, Lukman; Laboratory of Pharmacology and Toxicology, Department of Pharmacology and Clinical
Pharmacy, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Jogjakarta, Indonesia, 55281
Captopril is a selective inhibitor of angiotensin-converting enzyme (ACE) and is formulated by several pharmaceutical companies in Indonesia. This study was conducted to compare the bioavailability of a captopril tablet with reference products in healthy volunteers. The relative bioavailability of captopril was determined in single dose, randomized, crossover, and two-phase studies. The relative bioavailability of the test product (a generic captopril 50 mg tablet) with respect to the reference product was determined. Twelve healthy volunteers in two groups took part in these studies and took either the test or reference tablets in the first phase and received the other tablet in the second phase of each study. The bioavailability parameters include the peak concentration of captopril in serum (Cmax); the time to achieve the peak concentration (Tmax); and the area under the curve of captopril in serum versus time. Non-compartmental analysis on observed concentration versus time data has resulted in the mean value of Cmax of 545.26 ± 22.90 ng/mL (test product) and 548.91 ± 25.07 ng/mL (reference product) and mean Tmax of 1.13 ± 0.08 hours (test product) and 1.08 ± 0.08 hours (reference product), mean of AUC0-7 value of 1820.51 ± 75.31 ng. hour/mL (test product) and 1822.09 ± 99.29 ng. hour/mL (reference product), and mean of AUC0-inf value of 1967.83 ± 95.65 ng. hour/mL (test product) and 1996.94 ± 124.52 ng. hour/mL (reference product). Based on the data, it can be concluded that there is no significant difference (p>0.05) in bioavailability between both captopril Tablet (test and reference product).Key words: Bioequivalence, Captopril, HPLC, Human serum, Generic
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/681
10.14499/indonesianjpharm23iss3pp183-187
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 183-187
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/681/547
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1403
2022-10-26T01:05:53Z
3:ART
The capability of Several Population-based Approach Software to Analyze Sparse Drug Plasma Concentration Data after Intra-Venous Bolus Injection
Nugroho, Akhmad Kharis; Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta Indonesia
Hakim, Lukman; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, INDONESIA
Monolix, NONMEM, WinBUGS-PKBUGS, sparse data, therapeutic drug monitoring
Monolix, NONMEM, and WinBUGS-PKBUGS are among available software package for population-based modeling. The sparse condition of drug plasma concentration versus time (Cp-time) data is prevalent in clinically based studies involving patients. It is not ethical in this case, to collect a many and large volumes of blood samples. This study was aimed to simulate the capability of Monolix, NONMEM, and WinBUGS-PKBUGS to analyze very sparse Cp-time data after an intravenous bolus drug administration and to estimate the minimum number of Cp-time data required for an adequate analysis. Data of Cp-time were obtained based on simulation using the pharmacokinetic one-compartment open model following an intravenous bolus administration of 50 mg of a hypothetical drug. In this respect, six random values of k (rate constant of elimination) and Vd (volume of distribution) with mean and standard deviation values of 0.3 ±0.1 per hour and 30 ± 10 L, respectively, were used to create simulated Cp-time data of 6 subjects. Simulated Cp-time data in each subject were randomly ranked to choose data based on the intended number of samples in each subject. Several sparse Cp-time data scenarios, starting from a very limited state, i.e., with a total of 6 Cp-time data (1 datum per subject) to a rich situation with 48 Cp data (8 data per subject), were examined.The goodness of fit evaluations, as well as the similarity of individual values of k and Vd to the respective real values (p>0.05), indicate that nonlinear-mixed-effect-model using Monolix, NONMEM and WinBUGS-PKBUGS can appropriately describe sparse Cp-time data even with only 2 data per subject. This fact is an important finding to support the demand of analytical tool for a limited number of Cp-time data such as obtained in therapeutic drug monitoring event.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Hibah Penelitian Dasar Unggulan Perguruan Tinggi, a research grant scheme provided by the Ministry of Research, Technology and Higher Education, the Republic of Indonesia, year 2018.
2019-10-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1403
10.14499/indonesianjpharm30iss4pp293
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 293
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1403/923
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/442
2020-02-19T02:37:07Z
3:ART
A COMPARATIVE BIOAVAILABILITY OF FUROSEMIDE IN SOLID DISPERSIONS FORMS
Syukri, Yandi; Departement of Pharmacy
Faculty of Science and Mathematic
Islamic Indonesa University
Hakim, Lukman; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
Yuwono, Tedjo; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
Furosemide is a poorly soluble diuretic drug, the solubility of which can be enhanced by solid dispersion with polyvinylpirolidon (PVP). The solid dispersion system was prepared by a solvent method in various ratios of 1 : 5 and 1 : 7 of the drug and PVP, respectively, in order to improve furosemide bioavailability. The bioavailability of furosemide - PVP solid dispersion was compared with pure furosemide (control) and Lasix (reference). The study was done in a cross over design with a single-dose peroral that administered to the white male rabbits (n = 6). Furosemide blood levels were determined spectrofluorometrically by an extraction method. The area under the blood concentration-time curve AUC0 - , peak blood concentration Cmax and time to reach peak blood concentration Tmax were used to compare their bioavailabilities. The solid dispersion systems produced a higher extent of bioavailability than pure furosemide (P < 0,05). On the contrary, no statistically significant difference about the extent of bioavailability between solid dispersion and Lasix (P > 0,05). Finally, furosemide – PVP solid dispersion (1 : 7) was the best formulation with the highest extent of bioavailability and bioequivalence with the Lasix formula.Key Word : Solid dispersion, solubility, dissolution, bioavailability, furosemide.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/442
10.14499/indonesianjpharm0iss0pp28-32
Indonesian Journal of Pharmacy; Vol 12 No 1, 2001; 28-32
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/442/321
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1334
2019-10-10T01:29:33Z
3:ART
Profile of Biofilm-Producing Staphylococcus epidermidis from Intravenous Catheter Colonisation at Prof. Dr. Margono Soekarjo Hospital Purwokerto
Anjarwati, Dwi Utami; Jenderal Soedirman University
Siswandari, Wahyu; Dept of Pathology Clinic, Medical Faculty of Jenderal Soedirman University-Margono Soekarjo Hospital, Dr Gumbreg Nomor I, Medical Street, Mersi, Purwokerto, 53112
Peramiarti, IDSAP; Dept of Microbiology, Medical Faculty of Jenderal Soedirman University, Dr Gumbreg Nomor I, Medical Street, Mersi, Purwokerto, 53112
Biofilm, Intravenous catheter, colonization, Staphylococcus epidermidis
Biofilm- producing Staphylococcus epidermidis has evolved to be a significant human pathogen, particularly in the use of medical devices such as an intravenous catheter. Furthermore, biofilm-producing bacteria 10-1000 fold less susceptible to several antimicrobial agents than free-bacteria. This simple survey aimed to describe the profile of biofilm-producing S. epidermidis from intravenous catheter colonization of some patients in surgical and internal medicine wards at the hospital Margono Soekarjo, Purwokerto, and the antibiotics resistance pattern. A vitek® 2 compact (Enseval Medika Prima) was performed to identify the bacterial species and to examine the 73 antibiotics for understanding the resistance pattern automatically. Microtiter plate biofilm assay with crystal violet staining was performed to measure biofilm optical density (OD) for analyzing the biofilm production capabilities. A scanning electron microscopy (SEM) was done to compare the thickness of ultrastructure of biofilm-producing S. epidermidis visually. The present study found that 2 of 8 Gram-positive bacteria (25%) were biofilm-producing S. epidermidis. One of S. epidermidis was moderate whereas the other was high biofilm-producing bacteria. Images of SEM showed that a high biofilm-producing S. epidermidis has a thicker ultrastructure of biofilm than the moderate biofilm-producing, whereas a control, the weak biofilm-producing S. epidermidis ATCC 12228 has the least biofilm. Both of S. epidermidis strains were sensitive to Gentamicin, Moxifloxacin, Quinupristin/Dalfopristin, Linezolid, Vancomycin, Doxycycline, Minocycline, Tetracycline, Tigecycline, and Nitrofurantoin. Furthermore, both S. epidermidis strains were resistant to the other (63) antibiotics. In conclusion, two strains of S. epidermidis in this study have different capabilities to form the biofilm which were showed that high biofilm-producing strain was thicker than moderate biofilm-producing strain by scanning electron microscopy. However, both of them were resistant to the same number of antibiotics.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Prof. dr. Margono Soekarjo Hospital, Purwokerto
Research and higher education ministry, Indonesia
2019-03-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1334
10.14499/indonesianjpharm30iss1pp1
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 1
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1334/887
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1740
2021-01-06T13:13:53Z
3:ART
Cytotoxic Potential of Arthrospira platensis Extract on Cervical Cancer Cells Line Hela: Study on Antiproliferative, Cell Cycle, Apoptosis Induction and Anti Metastasis
Nurani, Laela Hayu; Faculty of Pharmacy, Universitas Ahmad Dahlan
Mahfudh, Nurkhasanah; Faculty of Pharmacy, Universitas Ahmad Dahlan
Gandjar, Ibnu Gholib
Rahayu, Intan; Faculty of Pharmacy, Universitas Ahmad Dahlan
A. platensis; cervical cancer; HeLa cells; HFDa cells
Cervical cancer can be treated conventionally with chemotherapy agents, but its use has side effects and complications in the form of damage to normal cells. This study aims to determine the potential of A. platensis as an alternative anticancer agent that is selective towards normal cells. Based on TLC analysis, A. platensis contains antioxidant compounds such as β-carotene, flavonoids, and terpenoids which are able to inhibit proliferation and trigger apoptosis of cancer cells. The study was conducted using cervical cancer cells HeLa and normal cells HDFa. A. platensis macerated with 96% ethanol at a ratio of 1:4. Based on probit analysis, it is known that ethanol extract of A. platensis has a cytotoxic effect on HeLa cells with IC50 values of 260.444μg/mL and index selectivity towards HDFa cells of 7.931. The mechanism of cytotoxic activity of ethanol extract of A. platensis is related to its ability to extend the doubling time, increase the induction of apoptosis, and reduce the rate of cells migration. Ethanol extract of A. platensis can also increase cells accumulation in the S phase to prevent cells from entering the G2/M phase.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-04-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1740
10.14499/indonesianjpharm31iss1pp19
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 19
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1740/939
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1740/281
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/515
2018-09-05T01:49:55Z
3:ART
FORMULATION AND EVALUATION OF MUCOADHESIVE BUCCAL TABLET OF DOMPERIDONE MALEATE
Dixit, Yuwaraj Dilipsingh; Sharad Pawar College of Pharmacy, Wanadongri, Hingna Road, Nagpur - 441 110, (MS), India.
Suruse, Pravin Babarao; Sharad Pawar College of Pharmacy, Wanadongri, Hingna Road, Nagpur - 441 110, (MS), India.
Shivhare, Umesh Dhaniram; Sharad Pawar College of Pharmacy, Wanadongri, Hingna Road, Nagpur - 441 110, (MS), India.
The objective of the present study was to develop and evaluate mucoadhesive dosage form of Domperidone maleate. It is an antiemetic synthetic benzimidazole compound that acts as a dopamine D2 receptor antagonist. Mucoadhesive Domperidone maleate tablet formulation was prepared by direct compression method. The formulation F3 containing (Carbopol 940 + sodium alginate) was found to be best among all the formulation batches because of its consistent release rate for 7 h and extent of drug release was 94.44%. Graphical treatment of the formulation F3 to Higuchi’s equation showed that the drug release was diffusion mediated. In-vitro permeability study for formulation F3 for 7 h had shown 76.69% drug release. FTIR studies showed no evidence on interaction between drug and polymers. Key words: Domperidone maleate, Mucoadhesive tablet, antiemetic
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2013-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/515
10.14499/indonesianjpharm24iss1pp47-55
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 47-55
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/515/393
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1221
2019-10-10T01:30:23Z
3:ART
Effect of Atorvastatin Treatment on Vascular Aterogenic Factors (Lipid Profiles and VCAM-1) in Patient Diabetes with Dyslipidemia
Mukti, Asri Wido; Airlangga University
Atorvastatin, Adhesion molecules, Diabetes mellitus, , dyslipidemia, VCAM-1.
To analyze effectiveness of atorvastatin 20mg on lipid profiles and adhesion molecule VCAM-1 in patient with diabetes dyslipidemia. An observational prospective cohort study was conducted from November 2016 to March 2017. Patients who fulfilled the inclusion criteria were taken twice for their lipid profiles and VCAM-1 measurements (before initiation of study and after 6 weeks treatment of atorvastatin 20mg). There were 13 patients who met the inclusion criteria. The results of 13 patients showed that after 6 weeks of atorvastatin therapy, there was a 28% decrease in total cholesterol (t0=223.77±49.69, t1=160.92±24.69), 39% LDL decrease (t0=152.59±44.25, t1 =93±21.44), a decrease in TG 38.6% (t0=200.85±101.53, t1=123.30±62.77) and a statistically significant decrease in VCAM-1 7.47% (t0=729.59±208.06, t1=675.06±182.88). The results of the correlation test between total cholesterol and VCAM-1 (p=0.185, r=0.268), LDL and VCAM-1 (p=0.127, r=0.307), TG and VCAM-1 (p=0.198, r=0.261) showed no correlation. Based on the results of the study, it can be concluded that atorvastatin therapy can provide improvements in atherogenic factors such as decreased lipid profile and VCAM-1, and there was no correlation between lipid profile and VCAM-1 in type 2 DM patients with dyslipidemia.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-04-11
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1221
10.14499/indonesianjpharm30iss2pp128-132
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 128-132
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1221/899
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1237
2020-03-18T10:32:19Z
3:ART
Analysis of Factors Influencing the Availability of Medicine During JKN Era
Satibi, Satibi; Faculty of Pharmacy, Universitas Gadjah Mada
Ranowijaya, Ranowijaya; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
Aswandi, Aswandi; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
Bermalam, Junagsti; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
Widodo, Gunawan Pamudji; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
JKN; availability of drugs factor; doctors factor; pharmacists factor and patients factor
The availability of medicine is important during National Social Healthy Insurance (JKN) that put into effect in the hospital, one of health service facility. The purpose of this study was to look of the availability of medicines was during JKN era and what factors influence the availability in three Region General Hospitals in Sulawesi. This study was conducted using descriptive method of analytic to get a correlation among doctors, pharmacists and patients as independent variables toward the availability of medicine as dependent variable. The study has been performed in 3 hospitals in Sulawesi. This data obtained were analyzed using statistical model of Linier Regression Analysis with F test or ANOVA test and t test. R was the value of regression which showed the influence of one to another variable. The results showed the level of medicine availability in hospital A was 12.49 months. Factors that influence the availability of medicines were doctors (R=0.778, p= 0.000), pharmacists (R=0.619, p= 0.000) and patients (R=0.653, p= 0.000). All there factor significantly affect the availability of medicines in Hospital A (R=0.675, p=0.000). Hospital B had an excess supply of medicines, with the average of 23.31 month. Factors that influenced the availability of medicines in Hospital B was doctor (R=0.877), pharmacist (R=0.861) and patient (R=0.984) (p=0.000 all). From multiple regression there factors showed a significant correlation to the availability of medicines (R=0.901, p=0.000). Similarity on Hospital A and B, data of the availability of medicine in hospital C was categorized excess as the average of stock was 56.01 month. Factors that influenced the availability of medicines in Hospital C was doctor (R=0.753, p= 0.000), pharmacist (R=0.869, p= 0.000) and patient (R=0.545, p=0.001).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-03-12
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1237
10.14499/indonesianjpharm29iss1pp37
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 37
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1237/837
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/512
2018-09-05T01:49:54Z
3:ART
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/676
2020-01-27T02:53:16Z
3:ART
DEVELOPMENT AND EVALUATION OF IN SITU GELS OF MOXIFLOXACIN FOR THE TREATMENT OF PERIODONTITIS
Kunche, Hareesh Babu; Department of Pharmaceutics, Sri Adichunchanagiri College of Pharmacy, B.G. Nagara, India
Ahmed, Mohammed Gulzar; Department of Pharmaceutics, Sri Adichunchanagiri College of Pharmacy, B.G. Nagara, India
Rompicharla, Narayana Charyulu; Department of Pharmaceutics, NGSM Institute of Pharmaceutical Sciences, Mangalore , India.
Gel dosage forms are successfully used as drug delivery systems to control drug release and protect the medicaments from a hostile environment. The main objective of the present investigation is to formulate and evaluate in situ gels of moxifloxacin for the treatment of periodontitis using gellan gum and sodium alginate based on the concept of ion activated systems. The system utilizes polymers that exhibit sol-to-gel phase transition due to change in specific physico-chemical parameters. Sol-to-gel transformation occurred in the presence of monovalent/divalent cations. It was found that increase in the concentration of calcium ions produced stronger gels. Formulations were evaluated for gelling capacity, drug content, clarity, viscosity, gel strength, spread ability, microbiological studies and in vitro release. The results shown by the above characterization studies were found to be satisfactory. Experimental part showed that viscosity of sols and gel strength was increased with increase in the concentration of polymers and the sustained release of drug was observed. The formulations were therapeutically efficacious, sterile and provided sustained release of the drug over a period of time. These results demonstrated that the developed system is an alternative to conventional drug delivery systems, provides patient compliance and economical.Key words: in situ gels, moxifloxacin, periodontitis, polymers, Ion activation.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/676
10.14499/indonesianjpharm23iss3pp141-146
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 141-146
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/676/542
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1487
2019-10-28T01:20:02Z
3:ART
Phytochemical analysis, antioxidant, antibacterial and antidiarrheal activities of Limnophila repens
Akter, Afroza; Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
Basher, Mohammad Anwarul; Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
Roy, Roni; Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
Sultana, Sharmin; Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
Daula, A F M Shahid Ud; Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
Limnophila repens; phytochemical content; antioxidant; antibacterial; antidiarrheal.
The study was carried out to determine the phytochemical content, antioxidant, antibacterial and antidiarrheal activity of methanolic extract of whole plant of Limnophila repens. The total phenolic, flavonoids, flavonols, tannins, β-carotene, lycopene, chlorophyll-α, and chlorophyll-β contents were found to be 65.21 ± 0.004 mg GAE/g , 44.83 ± 0.003 mg QE/g , 17.21 ± 0.002 mg QE/g, 56.27 ± 0.002 mg GAE/g , 1.32 ± 0.01 µg/g, 0.93 ± 0.012 µg/g, 11.85 ± 0.04 mg/g and 9.69 ± 0.037 mg/g, respectively. In vitro antioxidant potential of L. repens was assessed using various methods like by 2,2-diphenyl-1 picrylhydrazyl (DPPH), hydroxyl radical scavenging assay, reducing power assay, FRAP assay and thiobarbituric acid assay. IC50 values of the aforesaid tests were found to be 2.33 mg/ml, 442.31 µg/ml, 374 µg/ml, 55.09 µg/ml, and 4.30 mg/ml, respectively. The total antioxidant capacity was observed 140.7 ± 0.004 mg/g AAE. Aantibacterial activity of the methanolic extracts of whole plant of L. repens was evaluated using the disk diffusion method. The plant extract didn’t show any activity against Gram-positive (except Staphylococcus aureus) and Gram-negative bacteria. Finally, castor oil induced method was conducted to investigate the antidiarrheal activity of L. repens. At the dose of 400 mg/kg, the plant extract (35.62% inhibition of diarrhea) exhibited stronger effect compared to standard drug, loperamide (27.4% inhibition of diarrhea).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-09-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1487
10.14499/indonesianjpharm30iss3pp187
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 187
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1487/917
Copyright (c) 2019 Indonesian Journal of Pharmacy
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/258
2019-10-09T07:05:35Z
3:ART
IN VITRO EFFECT OF Chloroprocta SP. MAGGOTS SECRETION ON Staphylococcus epidermidis BIOFILM AND THE EXPRESSION LEVEL icaA OF GENE
Anjarwati, Dwi Utami; Lab. of Microbiology ,
Faculty Medicine and
Health Science. Jendral
Soedirman University.
Jl. Dr. Suparno-Purwokerto,
53122
Setiawati, Setiawati; Lab. of Farmacology Faculty
Medicine and Health Science
of Jendral Soedirman
University. Jl. Dr. SuparnoPurwokerto, 53122
Mujahidah, Mujahidah; Dept. of Microbiology Faculty of Medicine of
Diponegoro University – Kariadi Hospital. Jl. Doktor Soetomo No.14, 50244, Indonesia
Hapsari, Rebriarina; Dept. of Microbiology Faculty of Medicine of
Diponegoro University – Kariadi Hospital. Jl. Doktor Soetomo No.14, 50244, Indonesia
Nuryastuti, Titik; Dept. of Microbiology Faculty Medicine. Gadjah
Mada University. Yogyakarta, Sekip Utara
55281, Indonesia
Biofilm formation and the expression of icaA gene can be induced by environment conditions that are potentially toxic for bacterial cells. The effect of green flies maggots secretion to biofilm was studied some years ago to investigate in vitro effect of secretion of Chloroprocta sp. maggots on the formation of Staphylococcus epidermidis biofilm (phenotype) and the expression level of icaA gene (genotype) for indicating its mechanism on bacterial biofilm eradication. Microtiter plate biofilm assay was used to measure the effect of Chloroprocta sp. maggots secretion at various concentration on S. epidermidis biofilm. The expression level of icaA gene was performed by Real TimePCR using lightcycler method. The biofilm susceptibility test was done against maggots excretion/secretion using MTT assay. Whereas planktonic susceptibility testing was carried out using Kirby Bauer method. In the presence of maggots secretion at low concentration (5%), biofilm formation of S. epidermidis 734 was induced. In contrast, the expression level of icaA gene in production of maggots excretion/ secretion at concentration of 5% was lower than that of without maggots secretion (1/2 Fold). Eradication of bacterial biofilm was demonstrated after 48h incubation (MD=-0,011;P<0,05), but planktonic cell. In vitro difference effect of the Chloroprocta sp. maggots secretion at low concentration to phenotype and genotype of S. epidermidis biofilm showed that the possibility of maggots secretion ability to eradicate bacterial biofilm was not mainly due to the expression level of icaA gene.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2014-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/258
10.14499/indonesianjpharm25iss2pp76
Indonesian Journal of Pharmacy; Vol 25 No 2, 2014; 76
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/258/142
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1378
2019-10-03T03:16:50Z
3:ART
Synthesis and Molecular Docking Studies of N’-benzoylsalicylhydrazide derivatives as antituberculosis through InHA enzym inhibition
Santosa, Harry; University of Surabaya
Putra, Galih Satrio; University of Surabaya
Yuniarta, Tegar Achsendo; University of Surabaya
Budiati, Tutuk; Airlangga University
Antituberculosis, Benzoylsalicylhydrazide, Methyl Salicylate, InHA, Microwave synthesis, Molecular docking
The specific aims of this study is to synthesize and to study the possible mechanism of N’-benzoylsalicylhydrazide derivatives as an antituberculosis agent through InhA (Enoyl acyl carrier protein reductase) inhibition using in silico method. Five analogues of N’-benzoylsalicylhydrazide were synthesized using microwave irradiation from methyl salicylate as starting material, which yielded 80-90% product on average. This indicates a considerable improvement in terms of effectivity and efficiency, compared to the more conventional method using reflux condition. Character-ization of the compounds were subsequently carried out by UV, FTIR, 1H-NMR, 13C-NMR spectroscopy, which confirmed that the compounds had been successfully synthesized. Ultimately, molecular docking was performed using Molegro Virtual Docker (MVD) on the active site of InhA enzyme to predict the activity of the compounds. The results showed that all compounds performed comparatively well against N-(4-Methylbenzoyl)-4-benzylpiperidine as the native ligand and also yielded lower docking score than isoniazide (INH). From this study it can be concluded that N’-benzoylsalicylhydrazide derivatives could be synthesized using microwave irradiation with good product yield and all of the synthesized analogues are suggested to possess antituberculosis activity via InhA enzyme inhibition. In vitro activity will have to be determined in the future to validate whether N’-benzoylsalicylhydrazide derivatives perform well as a potential antituberculosis agent.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-11-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1378
10.14499/indonesianjpharm29iss4pp198
Indonesian Journal of Pharmacy; Vol 29 No 4, 2018; 198
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1378/884
Copyright (c) 2018 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/768
2018-11-15T06:52:02Z
3:ART
Investigation on drug dosage form : analysis of prescriptions available in pharmacy in kotamadya Yogyakarta
Wiedyaningsih, Chairun; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
., Oetari; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
The analysis of dispensing prescriptions available in Pharmacy in Yogyakarta was performed. The analysis included observation problems of dosage form preparations, and examination compounds of medicines which were mentioned in dispensing prescriptions. The study was conducted by sampling dispensing prescriptions from the pharmacy located in Yogyakarta. Questionnaires and interviews to pharmacists were also conducted in order to support the prescription data. The result showed that the oral solid dosage forms were the majority (71%) of dispensing prescriptions written. tablet crushing was the major problems of. The majority objective of therapy with this type of dispensing were for asthma, infection and allergy. It was also found that brand names (74%) medicine were more written than generic names. The most generic name written/added was CTM (chlorpheniramin maleat). Results of questionnaire evaluation showed that semi solid (unguentum, cream) was the major problem (69%) of pharmaceutical dispensing in the Pharmacy. Dispensing Salicylic acid with other semi solids were the most problems. Prescription analysis showed that about 14,2% dispensing prescriptions contain salycilic acid mixed with cream, and 26,5% dispense prescriptions contains salicylic acid mixed with any kinds of drugs or other semi solid forms. Liquid were the minor (7,2%) dispensing prescriptions written by physicians. However problems were still remain caused bycrushing coated tablets and dispensing to the liquid.Keywords : prescription, pharmaceutical stores, dosage forms, Yogyakarta
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/768
10.14499/indonesianjpharm0iss0pp201-207
Indonesian Journal of Pharmacy; Vol 14 No 4, 2003; 201-207
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/768/879
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/510
2018-09-05T01:49:54Z
3:ART
IDENTIFICATION OF BAWANG SABRANG (Eleutherine americana Merr. ex K. Heyne) IN INDONESIA BASED ON CHROMOSOME CHARACTERS
Daryono, Budi Setiadi; Genetic Laboratory Biology Faculty, Universitas Gadjah Mada Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
Rahmadani, Wenny Deisshinta; Genetic Laboratory Biology Faculty, Universitas Gadjah Mada Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
., Sudarsono; Department of Pharmaceutical Biology, Faculty of Pharmacy Universitas Gadjah Mada 55281,Indonesia
Bawang Sabrang (Eleutherine americana Merr. ex K. Heyne) is a plant belongs to Iris family (Iridaceae). Genetic study of the Eleutherine species should be investigated to yield valuable information for breeding program. The aim of this research was to determine chromosome characters as a preliminary research on the genetic characterization of Bawang Sabrang. Squash method on the root tips was used for chromosome preparation of this plant. The results showed that the time of cell division and prometaphase stages of Bawang Sabrang were occurred at about 08.00-08.30 a.m. and 08.20 a.m., respectively. Chromosome number of Bawang Sabrang was 2n=12 and the karyotype consisted of 8 (4 pairs) of metacentric chromosomes, 2 (1 pair) submetacentric chromosomes and 2 (1 pair) subtelocentric chromosomes which have the longest of total length chromosomes. Therefore, the karyotype formula of Bawang Sabrang was 2n=12=8m+2sm+2stSAT. Besides that, on the pair of subtelocentric chromosomes there was a satellite at each of the chromosome. Analysis of chromosome characters exhibited that the long of total length chromosomes was about 1.687 ± 0.111 µm to 5.320 ± 0.716 µm. Based on the R value ( 3,65 ± 0,41), it revealed that there was variation of chromosome size on this Eleutherine species in Indonesia. Moreover, data of the chromosome characters is important to complete the database of Bawang Sabrang as a potential medicinal herb in Indonesia Keywords: Bawang Sabrang, Eleutherine americana, chromosome, karyotype
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2013-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/510
10.14499/indonesianjpharm24iss1pp22-29
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 22-29
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/510/388
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1506
2019-10-10T01:29:33Z
3:ART
Inhibitory Activity of Sargassum hystrix Extract and Its Methanolic Fractions on Inhibiting α-Glucosidase Activity
Azizi, Wirdatul Auliya; Department of Fisheries Faculty of Agriculture Universitas Gadjah Mada Jalan Flora Gedung Perikanan A4 Bulaksumur Yogyakarta 55281
Ekantari, Nurfitri; Department of Fisheries Faculty of Agriculture Universitas Gadjah Mada Jalan Flora Gedung Perikanan A4 Bulaksumur Yogyakarta 55281
Husni, Amir; Department of Fisheries Faculty of Agriculture Universitas Gadjah Mada Jalan Flora Gedung Perikanan A4 Bulaksumur Yogyakarta 55281
α-Glucosidase, Fraction, Methanol, Sargassum hystrix
Seaweed has a great potential in the pharmaceutical field, one of them as antidiabetic. The purposed of this study was to know the inhibitory activity of Sargassum hystrix extract and its methanol fraction in inhibiting α-glucosidase activity. S. hystrix was extracted using methanol, then partitioned using chloroform, ethyl acetate, and methanol. Methanol fraction then separated by column chromatography to obtain the compound. The crude extract, the partitioned methanol fraction, and the column chromatography fraction were tested for its activity on inhibiting the α-glucosidase. The compounds of active fraction were analyzed using gas chromatography-mass spectrometry (GC-MS). The inhibitory activity (IC50) of the crude extracts and the partitioned methanol fraction were 0.35±0.05 and 0.02±0.00 (mg/mL), respectively. The column chromategraphy fractions that had an inhibitory activity to α-glucosidase were M2 (23.46±1.63%), M3 (30.88±4.53%), M4 (73.64±3.47%), and M7 (53.48±1.56%). GC-MS showed that the suspected compound which had inhibiting α-glucosidase in methanol fraction were 9-Octadecenoic acid, 1-Heptadecanecarboxylic acid,9,12-Octadecadienoic acid (Z, Z), and Octadecanoic acid methyl ester.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-03-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1506
10.14499/indonesianjpharm30iss1pp36
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 36
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1506/891
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
8b87651b32ab30b6e9194537b19906c9