2024-03-29T09:45:52Z
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/oai
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/414
2017-09-20T03:28:09Z
3:ART
Synthesis of Phenethyl-aza-ß-Lactam (1,2-Dimethyl4(R,S)-Phenethyl-[1,2]Diazetidin-3-One)
Ritmaleni, Ritmaleni; Faculty of Pharmacy, Gadjah Mada University, Yogyakarta Indonesia
Anggarwal, Varinder K; University of Bristol
Racemic spiro-epoxide, (7RS, 1RS, 3RS)-phenethyl-10-oxa-1,3-dithiaspiro[6,7]octane 1,3-dioxide 12, can be synthesised in four step reactions, sequences starting from the commercially available 1,3-dithiane 8. The synthesis of aza ß-lactam, 1,2-dimethyl-4(R,S)-phenethyl-[1,2]diazetidin-3one 13, was successfully carried out by opening the epoxide by 1,2-dimethyl hydrazine salt in moderate yield. Keywords : epoxide, aza-ß-lactam
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/414
10.14499/indonesianjpharm0iss0pp162-166
Indonesian Journal of Pharmacy; Vol 16 No 3, 2005; 162-166
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/414/294
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1235
2018-10-23T02:19:33Z
3:ART
Antimicrobial activity and Identification of fungus associated Stylissa flabelliformis sponge collected from Menjangan Island West Bali National Park, Indonesia
Setyowati, Erna Prawita; Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada
Pratiwi, Silvia Utami Tunjung; Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada
Purwantiningsih, Purwantiningsih; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Universitas Gadjah Mada
Samara, Oka; Faculty of Pharmacy, Udayana University
Sponge-associated fungi, Stylissa flabelliformis, Staphylococcus aureus, Escherichia coli, Candida albicans
The Fungus is a very important microorganism as a producer of bioactive secondary metabolites. Active substances of microbial origin have been sought through the process of screening methods to obtain antimicrobial compounds. The purpose of this study was to isolate fungi associated with sponge taken from Menjangan Island National Park West Bali (Indonesia) and identify fungi that have antimicrobial activity. Isolation of fungus from sponge was carried out by spread plate method using Saboroud Saline Agar medium. Each fungi will be tested to Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922 and Candida albicans ATCC 10231. Identification of fungi is based on the observation of macroscopic, microscopic and also using 16rRNA/ITS phylogeny tree. The results showed that S. flabelliformis sponge had 10 fungal isolates. Most of them have antimicrobial activity. The name associated with a sponge fungus is These 10 fungus are Aspergillus flavus strain UPMZ02, Aspergillus fumigatus strain CD1621, Trichoderma reesei strain JCM 2267, Aspergillus nomius strain KUB105, Aspergillus sp. strain TLWK-09, Aspergillus flavus strain MC-10-L, Penicillium sp. strain RMA-2, Aspergillus sp. strain TLWK-09, Aspergillus fumigatus and Trichoderma reesei strain TV221
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-11-27
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1235
10.14499/indonesianjpharm29iss2pp66
Indonesian Journal of Pharmacy; Vol 29 No 2, 2018; 66
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1235/847
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1529
2021-01-06T13:13:53Z
3:ART
Inhibition of Cell Cycle and Induction of Apoptosis y Ethanol Leaves Extract of Chrysanthemum cinerariifolium (Trev.) In T47D Breast Cancer Cells
Mutiah, Roihatul; Department of Pharmacy, Faculty of Medicine and Health Science, Maulana Malik Ibrahim Islamic State University of Malang
Inayatin, Alfiyah Laily; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
Annisa, Rahmi; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
Indrawijaya, Yen Yen Ari; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
Listiyana, Anik; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
C. cinerariifolium, apoptosis, cell cycle, T47D cells
Chrysanthemum cinerariifolium (C.cinerariifolium) is a plant of the Asteraceae family, which has been applied by the community as an ornamental plant and traditional medicine. In this study, the effect of C. cinerariifolium leaves extract on inhibition of cell cycle and induction of apoptosis in T47D breast cancer cells was tested and compared to the standard chemotherapy agent. The citotoxic activity of C. cinerariifolium leaves extract against T47D cancer cells and Vero normal cells was tested by MTT method. Profile of apoptosis and cell cycle were observed by flow cytometry method. Based on chemical compounds profil which is tested used TLC showed that C.cinerariifolium leaves extracts contained flavonoid and terpenoid chemical compounds. The result of cytotoxic test showed that leaves extract of C. cinerariifolium was able to inhibit the growth of T47D cancer cell at IC50 418.8μg/mL. Doxorubicin, extracted from Streptomyces peucetius used as treatment in several cancers including breast cancer. Doxorubicin could inhibit the growth of T47D cancer cells in 115.1μg/mL. The results of cell cycle analysis showed that the C. cinerariifolium leaves extract inhibited cell cycle in G0-G1 and S phase, whereas doxorubicin was able to inhibit cell cycle in G0-G1 phase but experienced cell accumulation in G2-M phase. The percentage of apoptosis in cycle was showed in M1 (sub G1) and M5 (multinuclear) phase which treatment of C. cinerariifolium leaves extract was higher than doxorubicin. Therefore, C. cinerariifolium leaves extract has potential activity as anticancer agent causes inhibition of cell cycle and induction apoptosis.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
This work supported by The Directorate General of Islamic Higher Education (DIKTIS) of Interdisciplinary Basic Research Grant numbers 3209/Un.3/HK.00.5/05/2018.
2020-04-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1529
10.14499/indonesianjpharm31iss1pp1
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 1
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1529/937
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/691
2020-01-27T02:53:31Z
3:ART
SYNTHESIS AND ANALGESIC ACTIVITY OF 6-(MNITROPHENYL)-4-SUSTITUTED BENZYLIDENE-4,5-DIHYDROPYRIDAZIN-3(2H)-ONE DERIVATIVES
Asif, Mohammad; Department of Pharmacy, GRD (P.G) Institute of Management and Technology, Dehradun,
248009, India.
Many research groups have been interested in 3(2H)-pyridazinones for the development of potential analgesic and antiinflammatory agents. Stimulated by these findings, three 6-(mNitrophenyl)-4-sustituted benzylidene-4,5-dihydropyridazin-3(2H)-ones (IVa-IVc) have been synthesized and evaluated for analgesic activity against hot plate method. Compounds IVa-IVc were prepared from 6-m-nitrophenyl-4,5-pyridazin-3(2H)-one by condensation with respective aldehydes. All compounds (IVa-IVc) having nitro phenyl and benzylidene groups at position 6 and 4 of the pyridazinone ring receptively. All compounds (IVa-IVc) showed significant analgesic activity when compare to control group and were found less potent than reference drug aspirin.Key words:Pyridazinones, analgesic, benzylidene, nitrophenyl.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/691
10.14499/indonesianjpharm23iss4pp254-258
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 254-258
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/691/557
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1349
2019-10-10T01:29:33Z
3:ART
The Properties of Brown Marine Algae Sargassum turbinarioides and Sargassum ilicifolium Collected From Yogyakarta, Indonesia
Artemisia, Rahma; Stikes Madani Yogyakarta
Jl. Wonosari KM 10, Karanggayam, Sitimulyo, Piyungan, Bantul, DIY
Nugroho, Akhmad Kharis; Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
Setyowati, Erna Prawita; Department of Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
Martien, Ronny; Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
Sargassum turbinarioides, Sargassum ilicifolium, Fucoidan, Thin Layer Chromatography (TLC), Fourier transform infrared (FTIR) spectroscopy
Brown marine algae are the prominent source of marine natural products that have bioactive metabolites. Sargassum turbinarioides and Sargassum ilicifolium were dominated in Indonesia as brown marine algae that well known as a source of fucoidan. The samples were collected from Gunungkidul, Yogyakarta, Indonesia. In this study, we investigated and identified the yield of aqueous crude and purified extracts using different extraction temperatures (60°C, 70°C, 80°C, 90 °C). The highest yield of S. turbinarioides crude extract (7.36%) was obtained at temperatures 90 and 80 °C while the highest yield of S. ilicifolium was 3.49 % at 80 °C. The presence of sulfate polysaccharide in Sargassum turbinarioides is 3,78 % and Sargassum ilicifolium is 2,93 %. Each of the extract was screened using phytochemical detection, Thin Layer Chromatography (TLC) and Fourier transform infrared spectroscopy (FT-IR) analysis. The phytochemical detection indicated that Sargassum ilicifolium has bioactive metabolites such as carbohydrates, proteins and amino acids, terpenoid, phenolic compounds, and flavonoids. The FTIR spectrum of the S. turbinarioides and Sargassum ilicifolium extract refer to the presence of ester sulfate groups through showing peaks at 1300 to 1200 cm-1 and 980 to 950 cm-1. The result indicated that Sargassum turbinarioides and Sargassum ilicifolium contain of sulfate polysaccharide were prospect a biological activities to use for the development of marine nutraceutical drugs especially as antioxidant.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Ministry of Research, Technology, and Higher Education, Republic of Indonesia and also Beasiswa Unggulan Dosen Indonesia - Dalam Negeri (BUDI-DN) from Lembaga Pengelola Dana Pendidikan (LPDP) for sponsorship and financial support
2019-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1349
10.14499/indonesianjpharm30iss1pp43
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 43
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1349/892
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/515
2018-09-05T01:49:55Z
3:ART
FORMULATION AND EVALUATION OF MUCOADHESIVE BUCCAL TABLET OF DOMPERIDONE MALEATE
Dixit, Yuwaraj Dilipsingh; Sharad Pawar College of Pharmacy, Wanadongri, Hingna Road, Nagpur - 441 110, (MS), India.
Suruse, Pravin Babarao; Sharad Pawar College of Pharmacy, Wanadongri, Hingna Road, Nagpur - 441 110, (MS), India.
Shivhare, Umesh Dhaniram; Sharad Pawar College of Pharmacy, Wanadongri, Hingna Road, Nagpur - 441 110, (MS), India.
The objective of the present study was to develop and evaluate mucoadhesive dosage form of Domperidone maleate. It is an antiemetic synthetic benzimidazole compound that acts as a dopamine D2 receptor antagonist. Mucoadhesive Domperidone maleate tablet formulation was prepared by direct compression method. The formulation F3 containing (Carbopol 940 + sodium alginate) was found to be best among all the formulation batches because of its consistent release rate for 7 h and extent of drug release was 94.44%. Graphical treatment of the formulation F3 to Higuchi’s equation showed that the drug release was diffusion mediated. In-vitro permeability study for formulation F3 for 7 h had shown 76.69% drug release. FTIR studies showed no evidence on interaction between drug and polymers. Key words: Domperidone maleate, Mucoadhesive tablet, antiemetic
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2013-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/515
10.14499/indonesianjpharm24iss1pp47-55
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 47-55
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/515/393
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1274
2018-10-23T02:18:57Z
3:ART
The Influence of Medication Days’ Supply on Adherence and Cost of Hypertensive Patients at Primary Health Care Centre
Mufarrihah, Mufarrihah; Faculty of Pharmacy, Universitas Airlangga
Setiawan, Catur Dian
Wathaniah, Nurul
Rahmaniah, Atikah
Maharani, Primanda Ayu
Mahmudah, Azzahroh Sifa’ Lailiyah
Achmad, Gusti Noorrizka Veronika
adherence, cost, hypertension, medication days’ supply, Primary Health Care Centre
Primary Health Care Centre is known have policy discrepancy with the supply of medicines. This can influence the adherence and costs incurred by patients. The study aimed to identify the influence for hypertensive patients at Primary Health Care Centre in Surabaya. This study was designed cross sectional and data collection was done by using ARMS questionnaires for the patient's adherence and interview for the cost. Cost was calculated based on the frequency of real visits and monthly patient expenditures. Direct medical cost, direct non medical cost, and indirect cost were included based on patients’ perspective. Direct observations were done to calculate the days of drug supplies. A total of 406 patients from 50 Health Care Centre were included in the study. Days of drug supplies ranged from 3 to 30 days; total ARMS scores ranged from 12 to 33; and the total cost obtained ranged from IDR 34,965 to IDR 239,528. The Spearman’s correlation test sought to obtain the significant influence of medication days’ supply on adherence with p value 0.0001 and r = -0.347. While medication days’ supply didn't show any significant influence on the cost. It will be significant if the patient met the visit schedule, that was p value 0.0001 and r = -0.247. It is recommended to prolong antihypertensive days’ supply in order to improve patients' adherence, as well as to reduce cost expensed by patients.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-12-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1274
10.14499/indonesianjpharm28iss4pp213
Indonesian Journal of Pharmacy; Vol 28 No 4, 2017; 213
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1274/854
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1257
2018-06-22T02:08:58Z
3:ART
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/660
2020-01-27T02:53:58Z
3:ART
ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF RED Psidium guajavaL. LEAVES GROWN IN MANOKO, LEMBANG - INDONESIA
Fidrianny, Irda; Departement of Biology Pharmacy, School of Pharmacy, Intitut Teknologi Bandung
Jl. Ganesha 10 Bandung-40132 Indonesia
Hartanti, Rika; Departement of Biology Pharmacy, School of Pharmacy, Intitut Teknologi Bandung
Jl. Ganesha 10 Bandung-40132 Indonesia
Raveendaran, Narmmatha; Departement of Biology Pharmacy, School of Pharmacy, Intitut Teknologi Bandung
Jl. Ganesha 10 Bandung-40132 Indonesia
Psidium guajava L. (Myrtaceae) is a well known plant in Malaysia and Indonesia. Its leaves extract was found to possess antidiarrhea, antimicrobial, hepatoprotective and antioxidant activities. Objective of this research is to isolate an antioxidant subtance from Red Psidium guajava L. leaves. The crude leaves was extracted using Soxhlet apparatus by gradual polarity of three different solvents, n-hexane, ethyl acetate and methanol. Antioxidant activity of each extract was tested by using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method. Total phenol, total flavonoid and total tannin content of the extracts were also measured. Ethyl acetate extract was fractionated using vacuum liquid chromato-graphy for fractionation. Purification was performed using TLC preparative. Isolate then characterized using specific spray reagent, UV-Vis spectrophotometry and infrared spectrophotometry. Crude drug of Psidiumguajava contained flavonoid, tannin, quinone, saponin and steroid/ triterpenoid. Antioxidant activity of ethyl acetate extract is 65.63% with total phenol 4.25%, total flavonoid 0.53% and total tannin 1.16%. Antioxidant compound N was isolated from ethyl acetate extract.Antioxidant compound N was supposed to be aglycone flavone that has OH at C -4’, C-5 and C-7.Key words: Psidium guajava L. , antioxidant activity, ethyl acetate extract
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/660
10.14499/indonesianjpharm23iss1pp36-40
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 36-40
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/660/526
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1062
2017-09-11T06:05:22Z
3:ART
AN LC- MS/MS METHOD FOR THE DETERMINATION OF OMEPRAZOLE ON PROTON PUMP INHIBITOR IN HUMAN PLASMA
Sudha, T.; Dept Pharma. Analysis, The Erode College of Pharmacy, Erode TamilNadu, India. 638112
Reddy, Kalan Kumar
Hemalatha, P. V.
Ravikumar, V. R.
A sensitive and selective liquid chromatographic method coupled with tandem mass spectroscopy (LC-MS/MS) was developed for the quantification of omeprazole in human plasma. Lansoprazole was used as internal standard with plasma samples, extracted using 10mM ammonium acetate. A centrifuged upper layer was then evaporated and reconstituted with Acetonitrile: mobile phase buffer 70:30%v/v. The reconstructed samples were injected into a C18 column purospher star 5µ. The mobile phase was composed of ACN: mobile phase buffer (5mm ammonium bicarbonate buffer) in the ratio of 70:30%v/v with flow rate 1.0mL/min. The mass spectrometer was operated using positive ion mode and turbo electro spray ionisation. Nitrogen was used as the nebulizer, curtain, collision and auxiliary gases. Using MS/MS with multiple reactions monitoring (MRM) mode, omeprazole was detected without severe interferences from plasma matrix. Detection of omeprazole in human plasma was accurate and precision. This method has been successfully applied to the study of omeprazole in human specimensKeywords: Proton pump inhibitor, omeprazole, lansoprazole, LC-MS/MS, liquid liquid extraction
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-03-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1062
10.14499/indonesianjpharm27iss2pp80
Indonesian Journal of Pharmacy; Vol 27 No 2, 2016; 80
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1062/773
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1545
2019-10-28T01:20:02Z
3:ART
Effect of 7-Hydroxy-2-(4- Hydroxy -3-Methoxyphenyl)-Chroman-4-one On Level of Mangan-Superoxide Dismutase (Mn-Sod) and Superoxide Dismutase 2 (Sod2) Gene Expression in Hyperlipidemia Rats
Ayunda, Rahmah Dara; Magister of Biomedical Science Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada
Prasetyastuti, Prasetyastuti; Departement of Biochemistry Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada
Hastuti, Pramudji; Department of Biochemistry Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada, Skip Utara 55281 Yogyakarta
Hyperlipidemia, flavonoids, Mn-SOD, SOD2 gene
Hyperlipidemia is a lipid metabolism disorder characterized by an increase in serum lipid levels. Hyperlipidemia is a major risk factor for many metabolic syndrome diseases because it triggers oxidative stress. Oxidative stress can be reduced by endogenous antioxidant enzymes triggered by exogenous antioxidant compounds, such as 7-OH-2- (4-OH-3-methoxyphenyl)-chroman-4-one isolated from the seeds of Swietenia macrophylla King. The aims of this study were to investigate the effects of 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one compounds on cholesterol level, LDL level, Mn-SOD levels and SOD2 gene expression of hyperlipidemic rats. Thirty rats (Rattus norvegicus ) were divided into 6 groups, normal group (N), hyperlipidemia group (HL), hyperlipidemia group with simvastatin (P), hyperlipidemic group with 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one with dose 10 (F10), 30 (F30) and 90 (F90) mg/200g body weight (BW). Cholesterol and LDL were analyzed with CHOD-PAP method, Mn-SOD level was analyzed by ELISA method and SOD2 gene expression was analyzed by qPCR method. The decrease in cholesterol and LDL levels were most prevalent in group F90 with dose 90 mg/200g BW of 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one, with average difference each of them was 172.43 mg/dL and 36.12 mg/dL. The rats fed on high-cholesterol diet exhibited a significant elevation in Mn-SOD levels (p<0.05) compared to normal group. The treated animals with 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one has the level of Mn-SOD is significantly lower (p<0.05) compared with hyperlipidemic group. Expression of SOD2 in group F90 has value close to normal group (p> 0.05). 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one with dose of 90 mg/200g BW improved cholesterol levels, LDL levels, Mn-SOD levels and SOD2 gene expression in hyperlipidemic rats.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-08-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1545
10.14499/indonesianjpharm30iss3pp180
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 180
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1545/915
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/674
2020-01-27T02:51:49Z
3:ART
IDENTIFICATION OF SAFETY ALERT BY MONITORING ANALYTICAL PARAMETERS AND HIGH-RISK DRUGS
Vilaplana, Vicente Escudero-; Pharmacy Department, Gregorio Marañón
University General Hospital Doctor Esquerdo 46, 28007 Madrid, Spain
Antúnez, María Gómez-; Internal Medicine Department, Gregorio
Marañón University General Hospital, Madrid, Spain
García, Esther Durán-; Pharmacy Department, Gregorio Marañón
University General Hospital Doctor Esquerdo 46, 28007 Madrid, Spain
Míguez, Antonio Muiño-; Internal Medicine Department, Gregorio
Marañón University General Hospital, Madrid, Spain
Sáez, María Sanjurjo-; Pharmacy Department, Gregorio Marañón
University General Hospital Doctor Esquerdo 46, 28007 Madrid, Spain
Early detection of adverse drug reactions (ADR) increases patient safety. Our objective was to identify ADR by monitoring laboratory parameters and high-risk drugs. We carried out a two-month prospective observational study in a Internal Medicine Department, with daily recording of drugs prescribed and the following parameters: Na, K, Ca, serum creatinine, glomerular filtration rate (GFR), INR, glucose, haemoglobin, platelets, ALT, AST, bilirubin, GGT, alkaline phosphatase, TSH, T4, and blood digoxin. High-risk drugs were closely monitored. 52 patients included, of whom 46.2% experienced an ADR. We observed an association with drugs in 25.5%, as follows: reduction in GFR, 26.9% (associated with loop diuretics [41.7%], angiotensin-converting enzyme [ACE] inhibitors [33.3%], angiotensin II receptor blockers [ARB] [16.6%], andanti-diabetic drugs [8.3%]); hypokalemia, 22.3% (associated with loop diuretics [50.0%], potassium-free fluid [37.5%], and salbutamol [12.5%]); hyperkalemia, 14.4% (associated with ACE inhibitors [60.0%] and ARB [40.0%]); INR out of range, 10.8% (associated with drug interactions [66.7%]); hyperglycemia, 8.1% (associated with corticosteroids [66.7%] and anti-diabetic drugs [33.3%]); and other conditions, 18.8%. We conclued that patient safety could be improved by implementing warnings in electronic prescriptions in cases of a decrease in GFR or modification of potassium levels in patients who are prescribed loop diuretics, ACE inhibitors, or ARBs.Key words: Adverse drug reaction, clinical decision support, high-risk drug, safety
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/674
10.14499/indonesianjpharm23iss2pp130-134
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 130-134
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/674/540
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1325
2018-11-02T06:46:53Z
3:ART
Free radical scavenging potential of Drosera indica L in presence of Dalton Ascites lymphoma (DAL) tumor bearing mice
Asirvatam, Raju
Christina, AJM
: Drosera indica L, Antioxidant, Dalton Ascites lymphoma, Malondialdehyde, Protein carbonyl content
The aim of the present study was to evaluate the antioxidant role of Drosera indica L. in Dalton Ascites lymphoma (DAL) bearing Swiss albino mice. The effect of ethanol and aqueous extracts of D.indica (EEDI and AEDI) were administered at a 250 and 500mg/kg once a day for 14 days, 24h after the inoculation of tumor cell line. After the treatment period, blood was collected from the animals and subsequently the animal were sacrificed for isolation of liver, brain, kidney and lungs for the observation of antioxidant status level. The parameters analyzed were catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), peroxidase (P), total protein (TP) and protein carbonyl content (PCC). Treatment with EEDI and AEDI significantly reduced the levels of MDA and increased the levels of GSH, SOD, CAT, P and TP in cancer induced animal and are similar as that of normal mice. The results suggest that the ethanol and aqueous extract of Drosera indica L. possess significant antioxidant effects in DAL bearing mice
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-07-27
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1325
10.14499/indonesianjpharm29iss3pp127
Indonesian Journal of Pharmacy; Vol 29 No 3, 2018; 127
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1325/874
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1155
2017-09-11T06:08:24Z
3:ART
SECONDARY BIOACTIVE METABOLITE GENE CLUSTERS IDENTIFICATION OF ANTICANDIDA-PRODUCING Streptomyces Sp. GMR22 ISOLATED FROM WANAGAMA FOREST AS REVEALED BY GENOME MINING APPROACH
Herdini, Camelia; Graduate School of Biotechnology, Universitas Gadjah Mada, Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine, Universitas Gadjah Mada
Mubarika, Sofia; Graduate School of Biotechnology, Universitas Gadjah Mada
Hariwiyanto, Bambang; Graduate School of Biotechnology, Universitas Gadjah Mada
Wijayanti, Nastiti; Graduate School of Biotechnology, Universitas Gadjah Mada
Hosoyama, Akira; Biological Resource Center, National Institute of Technology and Evaluation
Yamazoe, Atsushi; Biological Resource Center, National Institute of Technology and Evaluation
Nojiri, Hideaki; Biotechnology Research Center, The University of Tokyo
Widada, Jaka; Department of Agricultural Microbiology, Universitas Gadjah Mada
Streptomyces sp. GMR22; antifungal; genome sequence; antiSMASH
Streptomyces are a group of Gram-positive bacteria belonging to the Actinobacteria class, which are among the most important bacteria for producing secondary bioactive metabolites such as antibiotics, chemotherapeutics, insecticides and other high-value chemicals. Genome mining of gene clusters that encode the biosynthetic pathways for these metabolites has become a key methodology for novel compound discovery. Recently, we have isolated the Streptomyces sp. GMR22 from Cajuput rhizospheric soil at Wanagama Forest, Indonesia. GMR22 produced secondary metabolite that inhibited Candida albicans with IC50 of 62,5 μg/mL. The objective of this work was to reveal the novel secondary metabolites from GMR22 by genome mining approach. The antiSMASH 3.0 was used to predict gene clusters that encode the biosynthetic pathways of secondary metabolites in the genome of GMR22, and their core chemical structures. The pylogenomic analysis showed that GMR22 was closely related to Streptomyces bingchenggensis BCW1, as well as to the large genome size (9.5-12.7Mbp) groups of Streptomyces. AntiSMASH 3.0 analysis revealed that the genome of Streptomyces sp. GMR22 harbored at least 63 gene clusters that encode the biosynthetic pathways of secondary metabolites. It was the highest number of gene clusters had been observed among the members of Streptomyces groups, with PKS was predicted as the major groups of the identified gene cluster products. The results suggested that GMR22 could be a strong potential candidate of secondary bioactive metabolites source.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-03-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1155
10.14499/indonesianjpharm28iss1pp26
Indonesian Journal of Pharmacy; Vol 28 No 1, 2017; 26
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1155/801
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1470
2022-10-26T01:05:53Z
3:ART
Quality of Life of Patient with Hypertension in Primary Health Care in Bandar Lampung
Khoirunnisa, Sudewi Mukaromah; Institut Teknologi Sumatera
Akhmad, Atika Dalili; Institut Teknologi Sumatera
Hypertension; Quality of Life; SF-36
The high prevalence of hypertension in Indonesia, encourages studies related to how the quality of life of patients with hypertension. The purpose of this study was to measure quality of life, identify and explain factors related to the quality of life of patients with hypertension.The study design was descriptive correlation using a cross sectional study approach. The study subjects were all outpatient hypertensive patients several health centers in Bandar Lampung. The instrument used in this study was the Indonesian SF-36. The data collected included the patient's demographic characteristics including gender, age, education, occupation, and marital status, and the fields related to the health history of the study subjects included the duration of hypertension, complications, and the number of antihypertensive drugs consumed. Data were analyzed using bivariate analysis to see the relationship between two variables with the level of significance used was 5% (α = 0.05) with the value of the confidence interval set was 95%. Multivariate analysis was conducted to study the relationship of several independent variables with one or several dependent variables.The results of univariate analysis showed that age, marital status, duration of illness, complications, and the number of drugs consumed had an effect on the quality of life of hypertensive patients (p <0.05). The results of multivariate analysis showed that the factors of age, marital status, and duration of hypertension are factors that influence the physical domain, while the factors of gender, marital status, duration of hypertension, complications, and the number of drugs are influential factors in the mental domain mental.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
The Ministries of Research, Technology and Higher Education of Indonesia
2020-01-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1470
10.14499/indonesianjpharm30iss4pp309
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 309
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1470/927
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1470/200
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/686
2020-01-27T02:53:31Z
3:ART
CHOCOLATE FORMULATION AS DRUG DELIVERY SYSTEM FOR PEDIATRICS
Mayank, Sharma; College of Pharmacy, IPS Academy, Rajendra Nagar, A.B. Road, Indore - 452012 (M.P.), India
Kumar, Jain Dinesh; College of Pharmacy, IPS Academy, Rajendra Nagar, A.B. Road, Indore - 452012 (M.P.), India
Chocolate is highly sophisticated and infinitely a versatile food that can be combined to create completely different taste and texture sensations. The objective of the present study is to develop a palatable chocolate formulation of Domperidone and Cetirizine for pediatric administration and to increase patient’s desire to consume the medication. In present investigation chocolate base is prepared by use of cocoa powder, coco butter, lecithin, and pharmaceutical grade sugar. Thereafter drug is incorporated to prepared chocolate base. The medicated chocolate prepared is evaluated for appearance, moisture content determination using digital Karl Fischer titrator, blooming test, drug content determination & drug excipient interaction by differential scanning calorimetry.Key words: Medicated Chocolate, Pediatrics, Chocolate formulation
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/686
10.14499/indonesianjpharm23iss4pp216-224
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 216-224
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/686/552
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1367
2019-10-03T03:16:50Z
3:ART
α-Glucosidase Inhibition Activity of Stem Bark Ceiba pentandra Linn. In Vitro and In Silico
Syihabudin, Vicri; Pakuan University
α-glucosidase; AutoDock Vina; LCMS/MS; vavain derivative
Ceiba pentandra (L.) stem bark plant contains tannin, flavonoid, alkaloid, terpenoid, and saponin compounds. It is known that flavonoid compounds have a potential for inhibition activity of an α-glucosidase enzyme. The purpose of this research is to know the α-glucosidase inhibition activity of the fraction of n-hexane, ethyl acetate, and water. These three fractions measured α-glucosidase inhibition activity by spectrophotometric UV-vis method. The fraction extract with IC50 values can best be done an analysis of active compounds using LCMS/MS methods, and results of the suspected compound do the binding molecule with a receptor model isomaltase α-glucosidase uses AutoDock Vina so that bond can predict drug active compounds. The results of this research activity are inhibition of α-glucosidase stem bark in the fraction of n-hexane 4,60 μg/mL, ethyl acetate fraction 8,55 μg/mL and fractions of the water 5,61 μg/mL. Analysis of the compound fraction of water by LCMS/MS eluted at the retention time 3,61 seconds allegedly derivative compounds (+) catechin and on retention time 8,70 minutes allegedly derivative compounds vavain. Vavain derivative compounds, (+) catechin, acarbose and quercetin each has its own docking score of -8,1; -8,8; -6,2 and -7,6 kcal/mol and has a semblance of bonding on amino acid Glu276 which is a residue catalytic role in hydrolysis reactions. Equality on the sides of active chemical bonds is expected to have the same activity as drug inhibition of the enzyme α-glucosidase.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Bina Lohita Sari, Novi Fajar Utami, Purnama Alam and Utari
2018-12-17
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1367
10.14499/indonesianjpharm29iss4pp206
Indonesian Journal of Pharmacy; Vol 29 No 4, 2018; 206
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1367/885
Copyright (c) 2018 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/510
2018-09-05T01:49:54Z
3:ART
IDENTIFICATION OF BAWANG SABRANG (Eleutherine americana Merr. ex K. Heyne) IN INDONESIA BASED ON CHROMOSOME CHARACTERS
Daryono, Budi Setiadi; Genetic Laboratory Biology Faculty, Universitas Gadjah Mada Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
Rahmadani, Wenny Deisshinta; Genetic Laboratory Biology Faculty, Universitas Gadjah Mada Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
., Sudarsono; Department of Pharmaceutical Biology, Faculty of Pharmacy Universitas Gadjah Mada 55281,Indonesia
Bawang Sabrang (Eleutherine americana Merr. ex K. Heyne) is a plant belongs to Iris family (Iridaceae). Genetic study of the Eleutherine species should be investigated to yield valuable information for breeding program. The aim of this research was to determine chromosome characters as a preliminary research on the genetic characterization of Bawang Sabrang. Squash method on the root tips was used for chromosome preparation of this plant. The results showed that the time of cell division and prometaphase stages of Bawang Sabrang were occurred at about 08.00-08.30 a.m. and 08.20 a.m., respectively. Chromosome number of Bawang Sabrang was 2n=12 and the karyotype consisted of 8 (4 pairs) of metacentric chromosomes, 2 (1 pair) submetacentric chromosomes and 2 (1 pair) subtelocentric chromosomes which have the longest of total length chromosomes. Therefore, the karyotype formula of Bawang Sabrang was 2n=12=8m+2sm+2stSAT. Besides that, on the pair of subtelocentric chromosomes there was a satellite at each of the chromosome. Analysis of chromosome characters exhibited that the long of total length chromosomes was about 1.687 ± 0.111 µm to 5.320 ± 0.716 µm. Based on the R value ( 3,65 ± 0,41), it revealed that there was variation of chromosome size on this Eleutherine species in Indonesia. Moreover, data of the chromosome characters is important to complete the database of Bawang Sabrang as a potential medicinal herb in Indonesia Keywords: Bawang Sabrang, Eleutherine americana, chromosome, karyotype
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2013-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/510
10.14499/indonesianjpharm24iss1pp22-29
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 22-29
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/510/388
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1084
2017-09-11T06:09:26Z
3:ART
FORMULATION OF SELF-NANOEMULSIFYING DRUG DELIVERY SYSTEM OF BOVINE SERUM ALBUMIN USING HLB (HYDROPHILIC-LYPOPHILIC BALANCE) APPROACH
Winarti, Lina; Postgraduate Programme, Faculty of Pharmacy, Universitas Gadjah Mada
SNEDDS; BSA; hydrophllic surfactant; lypophilic surfactant; protein delivery
Self-Nanoemulsifying Drug Delivery System (SNEDDS) has potential to be developed for oral protein delivery because it is free from water, hence preserving the stability of protein, protecting protein from enzymatic degradation, and enhancing the protein permeability in the gastrointestinal tract (GIT). However, protein-based SNEDDS formulation is challenging due to low solubility property of protein in oil, which is towards zero. This present study aimed to obtain the most compatible SNEDDS system for protein using HLB approach. Bovine serum albumin (BSA) was used as a protein model in this study. A number of 78 formulae with HLB ranging between 11 and 15 were screened to acquire stable SNEDDS composition without the presence of phase separation. Of 13 stable formulae, two were selected (F30, F45) with HLB 15, and then loaded with BSA. Physical characteristics of both formulae were then evaluated and the results suggested that SNEDDS with single hydrophilic surfactant (F45) and HLB 15 was the best formula for protein template as the stability testing showed that phase separation and precipitation did not appear. It was robust to pH and dilution with percentage of transmittance of 96.40±1.05% and the droplet size of 180.9nm. F45 also had uniform distribution of droplets size since the polydispersity index was less than 0.1. The zeta potential of F45 was -0.12mv with loading efficiency 83.57±1.77%. The emulsifying time of F45 was > 2min due to the formation of crystalline gel that was difficult to disperse.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1084
10.14499/indonesianjpharm27iss3pp117
Indonesian Journal of Pharmacy; Vol 27 No 3, 2016; 117
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1084/789
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1988
2021-01-07T01:00:39Z
3:ART
In vitro Comparative Study for Anti-proliferative Activity of Some Plant Extracts, Fam. Apiaceae, on HeLa Cell Line
Gomaa, Sara E.; Medicinal and Aromatic Plants Research Dept., Horticulture Research Institute (HRI), Egypt. 9 ALgamaa Street, Giza, Egypt
Friedersdorf, Matthew; Molecular Genetics and Microbiology Dept., Duke University Medical Center, NC, USA
El Enshasy, Hesham Ali; Director,
Institute of Bioproduct Development (IBD), Universit Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Abou-Donia, MB; Pharmacology and Cancer Biology Dept., Duke University School of Medicine, NC, USA.
Apiaceae - Anti-proliferative - Extracts - HeLa Cell Line
In this research, the biological activities of five plant extracts from family Apiaceae; Italian Parsley (Petroselinum neapolitanum), Fennel (Foeniculum vulgare), Celery (Apium graveolens), Cilantro (Coriandrum sativum) and Dill (Anethum graveolens), were studied. Antiproliferative effect of eleven ethanol crude extracts was tested in Human Cervical (Hela) cancer cells. Results clearly demonstrated that all plant extracts showed high significant difference when compared to the negative control (DMSO).Parsley leaves extract, cilantro leaves extract and cilantro stems extract showed no significant difference with the positive control (Actinomycin D). As for, fennel bulb extracts, fennel stalks extracts, celery stems gave better results than the positive control with no significant difference through the 24, 48 and 72 h treatment. There were no significant difference between Fennel extracts and the positive control, which showed high effect on the cancer cells survival. There were no significant difference between both extracts of Cilantro leaves and stems through each time but the best result was after 72 h of treatments. Regarding Dill leaves and stems, cell numbers recorded no significant difference between the both on time dependent manner. Further investigation for ethanolic extracts of parsley leaves, fennel bulb, fennel stalks, celery stems, cilantro leaves and cilantro stems which showed better results than using the commercial drug Actinomycin D (25ml/ml) for 24 h treatment or less depending on concentrations manner. Also, further investigation on different types of cancer cell lines to avoid the toxic effect of chemotherapy.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Jack Kenee, Duke University, USA
2020-08-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1988
10.14499/indonesianjpharm31iss2pp108
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 108
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1988/946
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1032
2017-09-11T05:02:39Z
3:ART
DETERMINATION OF SIBUTRAMINE ADULTERATED IN HERBAL SLIMMING PRODUCTS USING TLC DENSITOMETRIC METHOD
Hayun, Hayun; Faculty of Pharmacy, Univ. Indonesia, Depok, West Java, Indonesia 16415.
Maggadani, Baitha P; Faculty of Pharmacy, Univ. Indonesia, Depok, West Java, Indonesia 16415.
Amalina, Nurul; Faculty of Pharmacy, Univ. Indonesia, Depok, West Java, Indonesia 16415.
Sibutramine HCl; Herbal Slimming Products; TLC, Densitometry; Validation Method
Determination of sibutramine adulterated in herbal slimming product using thin layer chromatography (TLC) densitometric method with TLC silica gel 60 F254 aluminium plate as stationary phase and mixture of toluen-diethylamine (10:0.3) as mobile phase has been developed. The calibration curve in the concentration range of 0.50 to 5.00 µg/spot showed good linier relationship (r2 = 0.9986). The limit of detection and quantitation (LOD and LOQ) were 217.5 ng and 724.9 ng/spot, respectively. The method gave satisfactory specificity, linierity, precision and accuracy validation criteria and was applied for determination of sibutramine in herbal slimming products obtained from several drugstrores in Depok City, West Java, Indonesia. Results of the determination showed that six of seven samples analyzed were detected containing sibutramine HCl with the concentration of 2.45 - 26.24 mg in a single dosage of slimming herbal products
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1032
10.14499/indonesianjpharm27iss1pp15
Indonesian Journal of Pharmacy; Vol 27 No 1, 2016; 15
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1032/763
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1462
2019-10-10T01:30:23Z
3:ART
Effect of Platelet-rich Plasma on Caspase-3 and IGF-1 mRNA expression in the diabetic rat testis
Istiqamah, Evi; Postgraduate Student of Master Biomedical Science, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Department of Midwifery, Faculty of Public Health, Universitas Muslim Indonesia Makassar, Indonesia
Rizal, Dicky Moch.; Department of Physiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Puspitasari, Ika; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
PRP, diabetic rat testis, caspase-3 mRNA expression, IGF-1, mRNA in expression
Testicular damage is a serious complication of diabetes mellitus resulting in male infertility, which is associated with caspase-3 and IGF-1 mRNA expression. Platelet-rich plasma (PRP), with its rich growth factor composition, has proven beneficial in regenerative therapy. It is believed that PRP has not been studied in testes for diabetes mellitus and there are no studies in the literature concerning the influence of PRP on expressions of growth factors in testes.The aim of this study was to investigate the efficacy of adjunctive PRP in insulin treatment for repair of testicular damage in a diabetic rat model. Diabetes was induced by administering single dose 60 mg/kg streptozotocin. Twenty Wistar male rats were divided into four groups: group 1, control group; group 2, diabetes without treatment; group 3, diabetes with treated insulin; and group 4, diabetes with treated insulin and PRP. Rats were euthanized after two weeks of treatment, and testes were taken for caspase-3 and IGF-1 mRNA expression measurements.Diabetes mellitus induction caused a significant increase in caspase-3 mRNA expression with p=0.049 and significant decrease in IGF-1 mRNA expression with p=0.004. There was no difference in caspase-3 and IGF-1 mRNA expression of the diabetic rat testis given insulin and PRP compared to without PRP.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-05-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1462
10.14499/indonesianjpharm30iss2pp98-104
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 98-104
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1462/902
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1059
2017-09-11T05:04:51Z
3:ART
PHYTOCHEMICAL CONSTITUENTS AND HYPOGLYCEMIC EFFECT OF GYMNEMIC ACID EXTRACTS FROM BIG AND SMALL LEAF VARIETIES OF Gymnema Sylvestre R.Br
Krishnamurthy, Ramar; C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Bardoli, Surat, Gujarat, India
Animasaun, D. A.
Patel, R. T.
Ingalhalli, R. S.
Phytochemical and hypoglycemic effects of two varieties of Gymnema sylvestre were studied. The leaves of Big-leaf (MCL) and the small-leaf (ZMGL) varieties were grown in Uka Tarsadia University, Bardoli, India. Water, ethanol, methanol, petroleum ether, haxane and chloroform were used for phytochemical extraction from the dried leaves. Gymnemic acid was extracted and purified from the two varieties by Thin Layer Chromatography (TLC). Four groups of matured whisker rats (six per group) were induced with high-glucose level. Three groups were treated with standard drug Glibenclamide (5mg/kg), gymnemic acid extracts from MCL and ZMDL respectively while the fourth group served as experimental control. Hypoglycemic activities of extracts were evaluated using animals’ blood sample. Result showed water and methanol are best solvents for phytochemical extraction from the plant and the yield is higher in ZMGL than MCL. Also, gymnemic acid yield varied. From the results, hypoglycemic activities from the animals’ blood revealed that treatments from both MCL and ZMGL are not significantly different from standard drug Glibenclamide. The study concluded that water or methanol is appropriate solvent for phytochemical extraction from G. sylvestre leaves and extract from both lines of the plant could be utilized to reduce blood glucose levelKeywords: phytochemical extraction, extraction solvents, gymnemic acid, hypoglycemic effects
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-03-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1059
10.14499/indonesianjpharm27iss2pp59
Indonesian Journal of Pharmacy; Vol 27 No 2, 2016; 59
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1059/770
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1467
2019-10-28T01:20:02Z
3:ART
Anticancer Molecules from Catharanthus roseus
Taher, Zarani M; Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Agouillal, Farid; 1. Research Unit on Analysis and Technological Development in Environment, URADTE, Centre de Recherche Scientifique et Technique en Analyses Physico-Chimiques, CRAPC, B.P. 384, RP 42004 Zone Industrielle Bou-Ismail, Tipaza, Algeria.
2. Laboratory of Reaction Engineering, LGR, Faculty of Mechanical Engineering and Process Engineering, Houari Boumediene University of Science and Technology, USTHB, PB 32 El Alia, 16075, Bab Ezzouar, Algiers, Algeria.
R, Lim J.; School of Chemical Engineering and Energy, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Marof, Aina Q; Department of Chemistry, Mathematics and Science Faculty, Universitas Negeri Jakarta, Jakarta, Indonesia
Dailin, Daniel Joe; 1. Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
2. School of Chemical Engineering and Energy, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Nurjayadi, Muktiningsih; Department of Chemistry, Mathematics and Science Faculty, Universitas Negeri Jakarta, Jakarta, Indonesia
Razif, Ezzaty NM; Faculty of Applied Sciences, Universiti Teknologi Mara Negeri Sembilan, Kampus Kuala Pilah, Pekan Parit Tinggi 72000, Kuala Pilah Negeri Sembilan, Malaysia
Gomaa, Sara E; Horticulture Research Institute, Agriculture Research Center, Egypt.
El Enshasy, Hesham Ali; Director
Institute of Bioproduct Development (IBD)
Universiti Teknologi Malaysia (UTM)
Skudai, Johor Bahru, Malaysia
C. roseus; Anticancer; Extraction; Phytochemical; Tissue culture
Catharanthus roseus is an important medicinal plant found in various parts of the world and the bioactive compound has been extracted and used as anti-cancer agent to treat the cancer over decades. However, the extraction of bioactive compound also results in the generation of large quantities of pollution with wasted solvents. Toxic pollution occurs when synthetic chemicals are discharged or natural chemicals accumulate to toxic levels in the environment, causing reductions in wildlife numbers, degrading ecosystem functions and threatening human health. This review covers the extraction and phytochemical obtained leading to chemical compounds related to anti-cancer property of C. roseus. Additionally, recent advances of using biological cell cultures were also addressed. Thus, this work can be used for further investigation of C. roseus to be undertaken in future for its anti-cancer property further development and efficient production in drug industry
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
MOE and UTM-RMC (Malaysia) through HICOE grant no. R.J130000.7846.4J262.
2019-07-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1467
10.14499/indonesianjpharm30iss3pp147
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 147
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1467/910
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/669
2020-01-27T02:51:49Z
3:ART
DIFFERENTIATION BETWEEN PORCINE AND BOVINE GELATIN IN CAPSULE SHELLS BASED ON AMINO ACID PROFILES AND PRINCIPAL COMPONENT ANALYSIS
Widyaninggar, Amalia; Research Center of Halal Products, Universitas Gadjah Mada, Yogyakarta 55281,
Indonesia
., Triwahyudi; Research Center of Halal Products, Universitas Gadjah Mada, Yogyakarta 55281,
Indonesia
Triyana, Kuwat; Research Center of Halal Products, Universitas Gadjah Mada, Yogyakarta 55281,
Indonesia
Rohman, Abdul; Research Center of Halal Products, Universitas Gadjah Mada
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
Gelatin used for capsule shells can be derived from porcine origin. Some religion like Islam prohibited their followers to consume any products containing porcine derivatives including porcine gelatin in the products. Consequently, some rapid and reliable techniques are continuously developed for detection of porcine gelatin in some pharmaceutical products like capsule shells. The aim of this study was to evaluate the possibility to use amino acid profile in combination with chemometrics of principal component analysis for detection and classification between gelatin in capsule derived from porcine and bovine. Based on score plot of first principal components (PC1) and second principal components (PC2), porcine and bovine gelatins in capsule could be apparently distinguished.Key words: bovine gelatin; porcine gelatin; amino acid profile; principal component analysis
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/669
10.14499/indonesianjpharm23iss2pp104-109
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 104-109
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/669/535
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1308
2018-10-23T02:19:33Z
3:ART
Protective Effect of Ethanolic Extract Tempuyung Leaf (Sonchus arvensis L.) in Gentamicin-induced Acute Tubular Necrosis on Wistar Rats
Imelda, Imelda -; Biomedical Science Master Program, Graduate School of Medical Sciences, Universitas Padjadjaran, Bandung
Achadiyani, Achadiyani -; Department of Anatomy, Physiology and Cell Biology,Faculty of Medicine, Universitas Padjadjaran, Bandung
Sekarwana, Nanan -; Department of Pediatrics, Faculty of Medicine, UNPAD-RSHS, Bandung
acute tubular necrosis, creatinine, gentamicin, tempuyung, Sonchus arvensis L.
Acute tubular necrosis (ATN) is the most common histopathologic findings of acute kidney injury (AKI). AKI is marked by the decrement of glomerular filtration rate, causing waste metabolism retention (creatinine). Gentamicin is used as it is the most common nephrotoxic agent in inducing ATN. Tempuyung (Sonchus arvensis L.) has been used in folklore medicine to ameliorate kidney problems as it contains antioxidants, two of which are flavonoid and phenolic acid. Yet, these active substances’ benefit on gentamicin induced ATN has not been investigated in Indonesia. This research aims to analyze protective effect of ethanolic extract tempuyung leaf (EET) on gentamicin-induced ATN based on histopathological study and creatinine serum level. True experimental laboratoric study was done with simple random design on male wistar rats, randomly divided into 4 groups (n=4). Control group (CMC 0.5% aquadest); Induction group (Gentamicin 80 mg/kgBW); 1st treatment group (EET 100 mg/kgBW + Gentamicin 80 mg/kgBW) and 2nd treatment group (EET 200 mg/kgBW + Gentamicin 80 mg/kgBW) for ten days. On the 11th day, blood was taken for creatinine measurement and kidneys collection for histopathological study. Histopathological examination on gentamicin-treated rats revealed degenerative changes in kidney tubules. Aside from that, gentamicin-treated rats also showed increment in creatinine serum level. Conversely, simultaneous administration of EET with Gentamicin ameliorated the nephrotoxic effects of gentamicin as confirmed from the significant improvement on histopathological changes and normalization of creatinine serum level. Co-administration of EET and gentamicin provides protection on gentamicin-induced ATN, based on histopathological feature and creatinine serum level.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
-
2018-05-11
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1308
10.14499/indonesianjpharm29iss2pp86
Indonesian Journal of Pharmacy; Vol 29 No 2, 2018; 86
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1308/870
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1308/142
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1308/143
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1308/144
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/812
2017-09-12T07:12:36Z
3:ART
PURIFICATION AND CHARACTERIZATION OF POLYSACCHARIDE FROM MICROALGAE BTM 11 AS INHIBITOR OF HEPATITIS C VIRUS RNA HELICASE
Mustopa, Apon Zaenal; Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km. 46 Cibinong 16911 Bogor.
Lages, Aksar Chair; Department of Aquatic Products Technology, Bogor Agricultural University, Kampus IPB Darmaga Bogor 16680, Indonesia.
Ridwan, Muhammad; Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km. 46 Cibinong 16911 Bogor.
Sukmarini, Linda; Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km. 46 Cibinong 16911 Bogor.
Susilaningsih, Dwi; Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km. 46 Cibinong 16911 Bogor.
Hasim, Hasim; Dept. of Biochemistry, Bogor Agricultural University, Jalan Agatis Kampus IPB Darmaga Bogor 16680, Indonesia.
Delicia, Delicia; Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km. 46 Cibinong 16911 Bogor.
Hepatitis C virus is one of the causative agents for HCV-related liver disease development with high virulence. Antiviral drugs can be discovered through molecular target-based therapy by finding the inhibitors for RNA helicase that play crucial role in viral replication. An inhibitor can be derived from polysaccharides produced by microalgae. In this study, polysaccharide from microalgae BTM11 which had inhibitory activity against RNA helicase have been purified and characterized.On the other hand, the RNA helicase was produced by E. coli BL21(DE3)pLyss harboring NS3 RNA helicase HCV gene in pET-21b plasmid. This enzyme then was purified by affinity chromatography and this purified enzyme was used for HCV RNA helicase inhibitory assay. Polysaccharide fractions were separated from the extract of BTM 11 using Sepharose 4B column chromatography. Inhibitor activity was measured using colorimetry ATPase assay based on releasing of phosphate inorganic. The results of SDS-PAGE and Western blot showed that the purified RNA helicase had a molecular weight of 54kDa. The highest inhibition activity of HCV RNA helicase (88 ± 2,4726%) was achieved at fraction 10 of purified polysaccharide. The HPLC result showed that compounds of polysaccharide active fraction were maltopentose (Rt 4.183) and glucose (Rt 5.673). Both of 1H-NMR and IR spectra showed hydroxyl and carbonyl groups that present in the polysaccharide structure. Key words: Hepatitis C Virus, RNA Helicase, Microalgae BTM11, chromatography, polysaccharide
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2015-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/812
10.14499/indonesianjpharm25iss3pp134
Indonesian Journal of Pharmacy; Vol 26 No 3, 2015; 134
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/812/749
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/681
2020-01-27T02:53:16Z
3:ART
A BIOAVAILABILITY STUDY OF INDONESIAN GENERIC TABLET OF CAPTOPRIL IN HEALTHY VOLUNTEERS
Nugroho, Agung Endro; Laboratory of Pharmacology and Toxicology, Department of Pharmacology and Clinical
Pharmacy, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Jogjakarta, Indonesia, 55281
Hakim, Arief Rahman; Laboratory of Pharmacology and Toxicology, Department of Pharmacology and Clinical
Pharmacy, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Jogjakarta, Indonesia, 55281
., Purwantiningsih; Laboratory of Pharmacology and Toxicology, Department of Pharmacology and Clinical
Pharmacy, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Jogjakarta, Indonesia, 55281
Hakim, Lukman; Laboratory of Pharmacology and Toxicology, Department of Pharmacology and Clinical
Pharmacy, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Jogjakarta, Indonesia, 55281
Captopril is a selective inhibitor of angiotensin-converting enzyme (ACE) and is formulated by several pharmaceutical companies in Indonesia. This study was conducted to compare the bioavailability of a captopril tablet with reference products in healthy volunteers. The relative bioavailability of captopril was determined in single dose, randomized, crossover, and two-phase studies. The relative bioavailability of the test product (a generic captopril 50 mg tablet) with respect to the reference product was determined. Twelve healthy volunteers in two groups took part in these studies and took either the test or reference tablets in the first phase and received the other tablet in the second phase of each study. The bioavailability parameters include the peak concentration of captopril in serum (Cmax); the time to achieve the peak concentration (Tmax); and the area under the curve of captopril in serum versus time. Non-compartmental analysis on observed concentration versus time data has resulted in the mean value of Cmax of 545.26 ± 22.90 ng/mL (test product) and 548.91 ± 25.07 ng/mL (reference product) and mean Tmax of 1.13 ± 0.08 hours (test product) and 1.08 ± 0.08 hours (reference product), mean of AUC0-7 value of 1820.51 ± 75.31 ng. hour/mL (test product) and 1822.09 ± 99.29 ng. hour/mL (reference product), and mean of AUC0-inf value of 1967.83 ± 95.65 ng. hour/mL (test product) and 1996.94 ± 124.52 ng. hour/mL (reference product). Based on the data, it can be concluded that there is no significant difference (p>0.05) in bioavailability between both captopril Tablet (test and reference product).Key words: Bioequivalence, Captopril, HPLC, Human serum, Generic
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/681
10.14499/indonesianjpharm23iss3pp183-187
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 183-187
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/681/547
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1388
2019-10-03T03:16:50Z
3:ART
Screening of Antibacterial and Anticancer Activity of Soft Corals from Togean Islands, Indonesia
Zubair, Muhammad Sulaiman; Department of Pharmacy, Faculty of Sciences, Tadulako University, Kampus Bumi Tadulako, 94118, Palu, Indonesia
Lallo, Subehan; Faculty of Pharmacy, Hasanuddin University, 90245, Makassar, Indonesia
Rusmiyanti, Rusmiyanti; Department of Pharmacy, Faculty of Sciences, Tadulako University, Kampus Bumi Tadulako, 94118, Palu, Indonesia
Nugrahani, Arsa Wahyu; Department of Pharmacy, Faculty of Sciences, Tadulako University, Kampus Bumi Tadulako, 94118, Palu, Indonesia;
Jantan, Ibrahim; School of Pharmacy, Taylor’s University, Lakeside Campus, 1 Jalan Taylors, 47500 Subang Jaya, Selangor, Malaysia
Softcorals, Togean Islands, anti-cancer, antibacterial
Soft corals (Octocorallia, Alcyonaceae) have been reported to possess diverse biological activities and unique structural chemistry. This study aims to screen the potential antibacterial and anticancer activity of some soft corals collected from Togean Islands, Central Sulawesi, Indonesia. They were Lobophytum sp, Sarcophyton sp, Sinularia sp 1, and Sinularia sp 2. All dried coral materials were extracted for 3 x 24 h by maceration method using methanol and then evaporated by rotary evaporator to obtain viscous extracts. The determination of antibacterial activity had been performed by well agar diffusion method against Staphylococcus aureus and Escherichia coli. Meanwhile, the cytotoxic activity was performed by MTT method, followed by apoptosis annexin V-FTIC assay agains. Identification for the presence of terpenoids was performed by vacuum p-anisaldehyde-sulphuric acid spraying reagent on thin layer chromatography (TLC). Sinularia sp2 extract have strongly inhibited S. aureus and E.coli with the diameter of inhibition range from 12.76mm and 17.86mm, respectively. Moreover, Sinularia sp2 extract possessed also cytotoxic activity against human breast adenocarcinoma (MCF-7) and colorectal carcinoma (HCT-116) with the IC50 of 46.807 and 47.186 μg/mL, respectively. Extract Sinularia sp 1 was found to have strongest cytotoxicity on human colon colorectal carcinoma (HCT-116) with the IC50 of < 1.505 μg/mL. Annexin V-FTIC assay clearly exhibited that the apoptosis mechanism is proposed by the extracts of Sinularia sp1 and Sinularia sp 2. Terpenoids were identified on both extracts suggesting for further purification and isolation for the bioactive terpenoid compounds.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-11-21
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1388
10.14499/indonesianjpharm29iss4pp173
Indonesian Journal of Pharmacy; Vol 29 No 4, 2018; 173
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1388/880
Copyright (c) 2018 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/474
2017-09-20T03:28:28Z
3:ART
Inhibitory effect of THPGV-0 on the histamine release from antigen-induced RBL-2H3 cells
Nugroho, Agung Endro; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy,
Gadjah Mada University, Sekip Utara Yogyakarta
Ritmaleni, Ritmaleni; Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Jogjakarta, Indonesia
Sahid, Novrizal Abdi; Pharmacology, Informational Biomedicine, Ehime University Graduate School of Medicine, Shitsugawa, Japan
Maeyama, Kazutaka; Pharmacology, Informational Biomedicine, Ehime University Graduate School of Medicine, Shitsugawa, Japan
Tetrahydropentagamavunon-0 (THPGV-0) is assumed to be main metabolite product of biotransformation process of PGV-0. THPGV-0 was synthesized by converting PGV-0 to the compound by hydrogenation with Pd/C as a catalyst. PGV-0 potently inhibited the histamine release from rat mast cells in vitro and in vivo, however, ironically only traces amount of compound was found in the blood. THPGV-0 is assumed to have important roles in the biological effects of PGV-0 in vivo. In present study, we investigated the antiallergy effect of THPGV-0 in compare to this of PGV-0 in vitro. The study was performed by using rat basophilic leukemia (RBL-2H3) cell line, a tumor analog of mucosal mast cells. DNP-BSA, an antigen, was used as an inducer for stimulating the histamine release from mast cells. In present study, THPGV-0 at low concentration did not succeed to inhibit the histamine release, but at higher concentration (30 and 100 M) showed strong effects. THPGV-0 at concentration of 100 M depleted the histamine release by 96.10 0.51%. In compare to PGV-0, THPGV-0 has higher efficacy but less potent. In the study, the possibilities of the spontaneous release from RBL-2H3 cells by the compounds were also observed. All concentrations of THPGV-0 as well as PGV-0 showed low spontaneous histamine release, less than 10 % of the total histamine contained in RBL-2H3 cells.Key words: tetrahydropentagamavunon-0, allergy, histamine, RBL-2H3 cells
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2010-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/474
10.14499/indonesianjpharm0iss0pp242-249
Indonesian Journal of Pharmacy; Vol 21 No 4, 2010; 242-249
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/474/353
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1236
2018-01-09T08:39:08Z
3:ART
DEVELOPMENT AND EVALUATION OF CONTROLLED RELEASE FORMULATION OF LAMIVUDINE BASED ON MICROPOROUS OSMOTIC TABLET TECHNOLOGY USING FRUCTOSE AS OSMOGEN
Sahoo, Chinmaya Keshari; Pharmaceutics Department, Faculty of Pharmacy, University College of Technology, Osmania University, Hyderabad, Telangana-500007
Rao, Surepalli Ram Mohan; Mekelle Institute of Technology, Mekelle University, Mekelle, Ethiopia
Sudhakar, Muvvala; Department of Pharmaceutics, Malla Reddy College of Pharmacy, Maisammaguda, Secunderabad, Telangana-500014
AIDS; wet granulation; in vitro drug release; stability study
The present study was undertaken to develop controlled release osmotic pump tablets of lamivudine a nucleoside reverse transcriptase inhibitor for the treatment of acquired immune deficiency syndrome (AIDS).The tablets were prepared by wet granulation method using controlled release polymer hydroxyl propyl methyl cellulose (HPMCE5 LV), MCC as diluent, starch as binder and fructose as osmogen. The coating solution of core tablets were prepared by using cellulose acetate,poly ethylene glycol 400,600,4000,6000 and acetone to quantity sufficient with sorbitol for different batches. The prepared tablets were evaluated for pre compression parameters, post compression parameters, in vitro drug release study and scanning electron microscopy study. Among the prepared formulations LF4 batch show 97.78% drug release in 12hrs.The in vitro release kinetics were analyzed for different batches by different pharmacokinetic models such as zero order, first order,Higuchi,Korsmeyer Peppas and Hixon Crowell model. Short term stability study at 40±2ºC/75±5% RH for three months on the best formulation was performed showing no significant changes in thickness, hardness, friability, drug content and in vitro drug release.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-08-22
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1236
10.14499/indonesianjpharm28iss3pp167
Indonesian Journal of Pharmacy; Vol 28 No 3, 2017; 167
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1236/834
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1903
2021-01-06T13:13:53Z
3:ART
In Vitro Antiplasmodial Activity and Cytotoxicity of Active Subfractions of Harmsiopanax aculeatus Leaves
Turalely, Rachel; Biotechnology Study Program, Graduate School, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Chemistry Education Study Program, Faculty of Teacher Training and Education Science, Pattimura University, Ambon, Maluku, Indonesia
Wijayanti, Mahardika Agus; Department of Parasitology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada,
Hertiani, Triana; Faculty of Pharmacy, Universitas Gadjah Mada
Mustofa, Musthofa; Department of Pharmacology and Therapy, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada Yogyakarta, Indonesia.
H. aculeatus, antiplasmodial activity, cytotoxicity, malaria, subfractions
Harmsiopanax aculeatus leaves, a medicinal plant with locally named kapur, have been used traditionally to treat malaria in Maluku, Indonesia. However, the scientific information of this plant is still limited. In our previous study, the methanol extract of this plant leaves have been proven to possess in vitro antiplasmodial activity. This study was conducted to evaluate in vitro antiplasmodial activity and cytotoxicity of subfractions of the plant leaves. Fractionation was performed using a column chromatography with Sephadex LH-20 as the stationary phase and methanol as the mobile phase. The subfractions obtained were then tested for in vitro antiplasmodial activity on a chloroquine-resistant FCR3 strain of Plasmodium falciparum using a visual method. Cytotoxicity was evaluated by using MTT assay. The in vitro antiplasmodial activity and cytotoxicity were expressed as IC50, calculated using probit analysis with SPSS 16 for windows. The results showed that the four subfractions tested have a high antiplasmodial activity with IC50 values of 0.09; 0.18; 0.01; and 0.77 µg.mL-1, respectively. In addition, these subfractions had IC50 values of >400 µg.mL-1 against Vero cells indicating that they were non-toxic. In conclusion, the subfractions of H. aculeatus leaves are very active and selective against P. falciparum. Further study will be conducted to isolate the active compounds.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-04-09
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1903
10.14499/indonesianjpharm31iss1pp51
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 51
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1903/944
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/951
2017-09-11T04:55:50Z
3:ART
PROTEIN PROFILE AND MDA SPERMATOZOA LEVELS OF HYPERGLYCEMIC MICE FED BY BEAN SPROUTS EXTRACT
Hidayat, Erwin; Biology Department of Mathematics and Science Faculty Semarang State University
Susanti, R.; Biology Department of Mathematics and Science Faculty Semarang State University
Marianti, Aditya; Biology Department of Mathematics and Science Faculty Semarang State University
Hyperglycemic conditions lead increased lipid peroxide and MDA which would damage structure and fluidity membrane so that impaired membrane function. Vitamin E on sprouts is an antioxidant that is capable of maintaining the integrity of cell membranes. Twenty eight mice which were grouped into 4 groups: control , P1, P2 and P3. Hyperglycemic treatment is by injecting of monohidrate alloxan dose of 150 mg/kgbw in group (P1, P2 and P3). Preparation and injecting of bean sprouts extract dose of 50 mg/20gbw to P2 and 100 mg/20gbw to P3 on 30 days.Taking of sperm to sperm protein isolation, and then analysis of protein molecular weight, testing MDA TBARS method. Based on the research results of the analysis protein profiles found in the control and P3 to be 8 protein bands is 72, 60, 49, 42, 35, 30, 28, and 23 kDa. While the P1 and P2 protein band 42 kDa are not found. Results of the analysis showed MDA significant different, control group is 2.43 nmol/ml, P1 7.89 nmol/ml, P2 5.19 nmol/ml and P3 3.29 nmol/ml. Concluded that the bean sprouts extract dois 100 mg/20ggw shows a significance influence on recovery protein profiles and MDA levels spermatozoa of hyperglycemic mice.Keywords: Protein profile, MDA, Hyperglycemic, Bean sprouts
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/951
10.14499/indonesianjpharm26iss4pp192
Indonesian Journal of Pharmacy; Vol 26 No 4, 2015; 192
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/951/757
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1521
2019-10-10T01:30:23Z
3:ART
The Effect of Thionamide to TRH, TSH, IL-4, T-REG, and Anti-TPO in Graves’ Disease
Decroli, Eva; Endocrinology and Metabolic Subdivision, Division of Internal Medicine, Dr. M. Djamil General Hospital/ Medical Faculty of Andalas University, Padang, West Sumatera, Indonesia
Elvira, Dwitya; Allergy and Immunology Subdivision, Division of Internal Medicine, Dr. M. Djamil General Hospital/ Medical Faculty of Andalas University, Padang, West Sumatera, Indonesia
Aprilia, Dinda; Endocrinology and Metabolic Subdivision, Division of Internal Medicine, Dr. M. Djamil General Hospital/ Medical Faculty of Andalas University, Padang, West Sumatera, Indonesia
TRH, TSH, T-reg, IL-4, anti-TPO
The most common cause of hyperthyroidism is Graves' disease. TRH and TSH are hormonal factors that modulate and control thyroid function in Graves' disease. In the immunological aspect, Graves' disease is played by the role of T-reg, IL-4, and anti-TPO. Graves' disease treatment goal is to inhibit thyroid hormone secretion by administering thionamide. The evaluation of this treatment is its hormonal and immunological aspects. To describe the effect of thionamide on serum TRH, TSH, IL-4, T-reg, and anti-TPO levels in Graves' disease. This study is a clinical trial study in 25 study participants. All study participants were given thionamide, namely PTU 300mg for three months and blood samples were taken for laboratory tests. Serum TRH, TSH, IL-4, T-reg FOXP3, and anti-TPO levels were examined by ELISA. The mean levels at the beginning and after three months of therapy are: serum TRH 92.589pg/mL and 115.944pg/mL; serum TSH 0.041mU/L and 0.223mU/L; serum IL-4 19.759pg/mL and 23.040pg/mL; T-reg FOXP3 gene polymorphism 0.621ng/mL and 0.518 ng/mL; serum anti-TPO 2697.539pg/mL and 2604.710pg/mL. Increased levels of serum TRH and TSH levels were statistically significant. The change in serum IL-4, T-reg FOXP3 gene polymorphism, and anti-TPO levels were not statistically significant. The administration of thionamide in Graves' disease for three months will significantly decrease Wayne index and serum FT4 levels, increase serum TRH and TSH levels.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-05-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1521
10.14499/indonesianjpharm30iss2pp122-127
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 122-127
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1521/897
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/664
2020-01-27T02:53:58Z
3:ART
PHYTOCHEMICAL SCREENING AND ANALYSIS POLYPHENOLIC ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF WHITE DRAGON FRUIT (Hylocereus undatus)
VH, Elfi Susanti; Department of Chemistry, PMIPA FKIP, Universitas Sebelas Maret. Jl. Ir Sutami
36 A Surakarta Indonesia, 57126
Utomo, Suryadi Budi; Department of Chemistry, PMIPA FKIP, Universitas Sebelas Maret. Jl. Ir Sutami
36 A Surakarta Indonesia, 57126
Syukri, Yandi; Departement of Pharmacy, FMIPA, Universitas Islam Indonesia Jl. Kaliurang KM 14,5 Sleman Yogyakarta 55584
Redjeki, Tri; Departement of Pharmacy, FMIPA, Universitas Islam Indonesia Jl. Kaliurang KM 14,5 Sleman Yogyakarta 55584
White dragon fruit is a well known and widely used herbal medicine, especially in Asia, which contains several interesting bioactive constituents and possesses health promoting properties. The aim of this study was to analyze for the bioactive compounds, evaluate total phenolic contents and antioxidant capacities of methanolic extract of white dragon fruit. The antioxidant activity was determined by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay. Total phenolic content were determined by Folin-Ciocalteu method. Phytochemical screening of the white dragon fruit showed the presence of triterpenoid, alkaloid, flavonoid and saponin. The extract exhibited strong antioxidant activity with IC50 of 193 μg/mL, and total phenolic content of 246 μg/mL in 1 Kg dry extract.Key words: antioxidant activity, total phenolic, DPPH, white dragon fruit
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/664
10.14499/indonesianjpharm23iss1pp60-64
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 60-64
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/664/530
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1101
2018-10-23T02:19:20Z
3:ART
Formulation and Evaluation of Glipizide Tablets Utilizing Hibiscus Rosasinensis Leaves Mucilage
Bahadur, Sanjib; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Roy, Amit; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Baghel, Pragya; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Choudhury, Ananta; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Saha, Suman; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Chanda, Ranabir; Bengal School of Technology, Sugandha, Delhi Road, Hooghly, West Bengal, 712102
This research work aims to develop glipizide tablets using Hibiscus rosasinensis leaves mucilage. The mucilage was extracted by using double distilled water and precipitated with ethanol. The precipitated mucilage was dried and grounded into powder. The tablet utilizing the mucilage as excipient was prepared by wet granulation method. The tablets were subjected to various tests. The evaluatory parameters of tablets were found to be within the limits as per United States Pharmacopoeia NF 24/19. FTIR spectrum reveals that there is no incompatibility between the ingredients used. Diabetes was induced in wistar albino rats using streptozotocin and effect of formulation on blood glucose level was determined. It was observed that the formulation could not sustain the release of glipizide. However, the glipizide release was retarded as the amount of mucilage was increased. It was observed that on the completion of antidiabetic study, the formulation could bring the blood glucose level to normal in group rats. However, the blood glucose level was still elevated in group administered with pure gliplizide. It can be concluded that HRM can be used to formulate glipizide tablets. The formulations with HRM shows better hypoglycemic activity and this may be attributed to antidiabetic activity of Hibiscus rosasinensis.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-03-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1101
10.14499/indonesianjpharm29iss1pp23
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 23
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1101/860
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/817
2017-09-12T06:51:16Z
3:ART
DETERMINATION OF ELEMENTAL IMPURITIES IN SOME COMMERCIAL PAEDIATRIC AND ADULT FORMULATIONS OF ARTEMETHER – LUMEFANTRINE IN THE NIGERIAN MARKET BY ATOMIC ABSORPTION SPECTROSCOPY
Awofisayo, Sunday O; Department of Clinical Pharmacy and Biopharmacy, Faculty of Pharmacy, University of Uyo, P. O. Box 4274, Uyo Akwa Ibom State, Nigeria
Okhamafe, Augustine O; Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Benin, Benin City, 300001, Nigeria
Arhewoh, Mathew I; Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, University of Benin, Benin City, 300001, Nigeria
The composition of elemental impurities in paediatric powder for suspension (PPS) and double strength tablet (DST) commercial anti-malarial formulations of artemether-lumefantrine (AL) were determined. Six randomly selected PPS and DST products from a list of marketed products were purchased from a registered Pharmacy outlet and analyzed using flame atomic absorption spectrophotometer. The heavy metal levels were compared with the official limits for permitted daily exposure. The paediatric and adult products of AL presented the same types and number of elements with a total of 10 metals and levels ranging from 0.001-0.016 and 0.001-0.017ppm for the PPS and DST formulation, respectively. One of the DST and PPS products had significantly higher levels of cadmium, copper, chromium, nickel and cobalt compared with their respective formulation types (p<0.05). There was significant difference in the levels of chromium, nickel and cobalt but no difference in the levels of cadmium, lead, zinc and arsenic when DST and PPS products were compared (p<0.05). The drug products presented elemental oral exposure significantly lower than the permitted daily exposure. The levels of elemental impurities are satisfactory with respect to safety requirements. These impurities may adversely affect drug stability, shelf-life and bioequivalence of the products.Key words: Elemental impurities, Permitted Daily Exposure, Artemether-lumefantrine, Paediatric Powder for Suspension, Double Strength Tablets
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2015-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/817
10.14499/indonesianjpharm25iss3pp171
Indonesian Journal of Pharmacy; Vol 26 No 3, 2015; 171
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/817/744
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1118
2017-09-11T06:09:06Z
3:ART
SYNTHESIS AND ANTIMICROBIAL EVALUATION OF 2-[(SUBSTITUEDPHENYL)-5-(1-PHENYL-3-(PIPERAZINYL)PYRIDO[3,2-f]QUINAZOLIN-4(1H)-YL]-1,3,4-THIADIAZOLES
Ram, Tilak; Department of Chemistry, Govt. P.G. College, Uttarkashi-249193, U.K., India
Dubey, Ranjana; Department of Chemistry, S.R.M. University, Modinagar-201204, Meerut, U.P., India
Chaudhary, Nidhi; Department of Chemistry, M.I.E.T., Meerut-250001, U.P., India
Several novel 2-[(substitutedphenyl)-5-(1-phenyl-3-(piperazinyl)pyrido[3,2-f] quinazolin-4(1H)-yl]-1,3,4 thiadiazole derivatives have been designed and synthesised by incorporating four known moieties such as 2-methylquinolin-5-ol, acetophenone, urea and 3-substitutedphenyl-4-amino-5-mercapto triazoles by using multi-step conventional reaction strategy. The synthesized derivatives were characterized by IR, 1H-NMR, Mass and elemental analysis (C, H, N). Furthermore the synthesized 2-[(substitutedphenyl)-5-(1-phenyl-3-(piperazinyl)pyrido[3,2-f]quinazolin-4(1H)-yl]-1,3,4-thiadiazoles 4a-g were screened for antibacterial and antifungal activities. The bacterial panel consisted of Staphylococcus aureus, Escherichia coli, Klabsiella pneumoniae, Proteus vulgaris while fungal panel included of Aspergillus fumigatus (plant isolate), Candida glabrata, Candida albacans, Candida krusei. Ampicillin trihydrate and fluconazole were used as reference drugs for antibacterial and antifungal activity respectively. Bacterial and fungal inhibition determined by disk diffusion and serial plate dilution method respectively. Pathogenic inhibitions were determined by measuring the diameter of the inhibition zone in mm. Compound 4c demonstrated significant antibacterial and antifungal spectrum against all the tested microbes.Keywords- antibacterial, antifungal and substituted 1,3,4-thiadiazoles.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-09-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1118
10.14499/indonesianjpharm27iss4pp183
Indonesian Journal of Pharmacy; Vol 27 No 4, 2016; 183
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1118/791
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1564
2022-10-26T01:05:53Z
3:ART
Response Surface Methodology used in the Optimization of RP-HPLC Condition for Quantitative Analysis of Carmine and Rhodamine B
Nuvitasari, Reyna
Rohman, Abdul; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Martono, Sudibyo
The objective of this study was to optimize reversed-phase high-performance liquid chromatography (RP-HPLC) using an experimental design approach based on the response surface methodology of Central Composite Design (CCD) for separation and analysis of carmine (CAR) and Rhodamine B (RHO) in lipstick products. Some factors (independent variables) responsible for RP-HPLC separation including pH of buffer phosphate (X1), the acetonitrile ratio (X2), flow rate of mobile phase (X3), and column temperature (X4) were investigated. While, the responses (dependent variables) evaluated were resolution between CAR and RHO (Y1), tailing factor of CAR (Y2), tailing factor of RHO (Y3), retention time of CAR (Y4), retention time of RHO (Y5), peak area of CAR (Y6) and peak area of RHO (Y7). CCD showed that separation of CAR and RHO was influenced by these independent variables (factors). The optimum predicted conditions for the separation of CAR and RHO based on statistical results was pH buffer of 3.4, ACN 55%, the flow rate of 1.1 mL/min and column temperature of 35oC with the desirability of 1. Both CAR and RHO were clearly separated using optimum conditions, as suggested by CCD. The developed techniques were effective for optimizing chromatographic separation, therefore, the time consumption and a large number of running could be hindered.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-10-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1564
10.14499/indonesianjpharm30iss4pp276
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 276
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1564/922
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/430
2020-02-19T02:37:07Z
3:ART
NEW 6-(3-INDOLYL)BENZO[B]CARBAZOLES
Santoso, Mardi; Chemistry Department, Faculty of Science,
Sepuluh Nopember Institute of Technology Surabaya
Some attempts have been carried out to synthesize polycyclic heterocyclic compounds derived from new benzocarbazoles (1). New 6-(3-indolyl)benzo[b]-carbazoles (2a) have been synthesised in good yields from the reaction of simple indoles and o-phthaldialdehid in methanol catalysed by p-toulenesulfonic acid monohydrate. Their precise structure were established by microanalysis, spectroscopy, and X-ray crystallography of the benzo[b]carbazole (2a).Keywords: indoles, polycyclic heterocyclic compounds, benzo[b]carbazoles
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/430
10.14499/indonesianjpharm0iss0pp1-9
Indonesian Journal of Pharmacy; Vol 12 No 1, 2001; 1-9
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/430/309
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1273
2018-10-23T02:18:57Z
3:ART
Review of Insulin Therapy In Type 2 Diabetes Mellitus Ambulatory Patients
Suprapti, Budi; Faculty of Pharmacy Universitas Airlangga, Jalan Dharmawangsa Dalam, Surabaya, Indonesia
Widyasari, Nia; Faculty of Pharmacy Universitas Airlangga, Jalan Dharmawangsa Dalam, Surabaya, Indonesia
Rahmadi, Mahardian; Faculty of Pharmacy Universitas Airlangga, Jalan Dharmawangsa Dalam, Surabaya, Indonesia
Wibisono, Cahyo; Faculty of Medicine Universitas Airlangga, Jalan Dr. Moestopo, Surabaya, Indoensia
Diabetes mellitus, insulin, ambulatory patient, DUR, DRP
The purposes of this study were to review utilization of insulin therapy in type 2 diabetes mellitus out patients and identify its drug related problems. The data were collected cross-sectionally with purposive sampling method in the period March 2016 until May 2016 in Outpatient Clinic Universitas Airlangga Teaching Hospital Surabaya. The results of 240 patients showed that insulin was used as monotherapy insulin in 2,9% patients; combination 1 insulin & 1-4 OAD in 31,3%; basal bolus therapy 27,9%; combination basal−bolus therapy & 1-3 OAD 43,9%. Based on blood glucose target achievement, only 20,8% of patients achieve the target, 75,1% failed to achieve the target and 4,1% suffered from hypoglycemia. Drug related problems identified adverse drug reaction of antidiabetic therapy such as hypoglycemia (6.7%), nausea (3.8%), bloating (1.3%), increase of flatulency (2.9%) and inappropriate combination (0,4%). In conclusion insulin therapy was complicated and individually, most of the patients still did not reach the target and there was potential drug related problem in this patients group. So that caring from solid inter-professional health collaboration is needed
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Faculty of Pharmacy Universitas Airlangga, Jalan Dharmawangsa Dalam, Surabaya, Indonesia
2017-12-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1273
10.14499/indonesianjpharm28iss4pp221
Indonesian Journal of Pharmacy; Vol 28 No 4, 2017; 221
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1273/855
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1601
2021-01-07T01:00:39Z
3:ART
FTIR Method for Peptide Content Estimation and Degradation Kinetic Study of Canarium Nut Protein
Oktaviary, Rozana
Nugrahani, Ilma
Surantaatmadja, Slamet Ibrahim
Kartawinata, Tutus Gusdinar
Apsari, Cintya Nurul
Canarium nut; peptide; degradation; FTIR; kinetic
Quantitative analysis of bioactive peptide mostly conducted by measuring the activity. While the determination of peptide content in natural sources has been conducted using various instruments, vibrational spectroscopy remains underutilized. Here, we attempted new developed method in peptide quantification and degradation kinetic analysis using Fourier-Transform Infrared Spectroscopy. Bovine Serum Albumin was used as standard protein in method development and validation. Peptide content was estimated by converting peak area to concentration. The method was used to estimate peptide content in Canarium nut protein and its hydrolysates, which potentially hold biological activity. Kinetic study was conducted with microwave as an accelerator for hydrolysis, an apparatus rarely used in peptide study. Amide I band on wavenumber range of 1724.05-1619.91 cm-1 was selected for analysis, considering its selectivity and linearity. The method also met other validation requirement, including accuracy and precision. When applied in quantitative analysis, the method was able to calculate peptide content decrease in Canarium nut protein after hydrolysis using papain (38.24%), pepsin (33.67%) and alkaline reagent (28.53%). In kinetic study, microwave-assisted peptide degradation exhibited logarithmic profile with the equation of y=-0.148ln(x)+0.9591 and R² value of 0.963. Based on these results, FTIR is useful in estimating peptide content and in analyzing degradation kinetic profile.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-07-10
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1601
10.14499/indonesianjpharm31iss2pp78
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 78
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1601/949
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/768
2018-11-15T06:52:02Z
3:ART
Investigation on drug dosage form : analysis of prescriptions available in pharmacy in kotamadya Yogyakarta
Wiedyaningsih, Chairun; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
., Oetari; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
The analysis of dispensing prescriptions available in Pharmacy in Yogyakarta was performed. The analysis included observation problems of dosage form preparations, and examination compounds of medicines which were mentioned in dispensing prescriptions. The study was conducted by sampling dispensing prescriptions from the pharmacy located in Yogyakarta. Questionnaires and interviews to pharmacists were also conducted in order to support the prescription data. The result showed that the oral solid dosage forms were the majority (71%) of dispensing prescriptions written. tablet crushing was the major problems of. The majority objective of therapy with this type of dispensing were for asthma, infection and allergy. It was also found that brand names (74%) medicine were more written than generic names. The most generic name written/added was CTM (chlorpheniramin maleat). Results of questionnaire evaluation showed that semi solid (unguentum, cream) was the major problem (69%) of pharmaceutical dispensing in the Pharmacy. Dispensing Salicylic acid with other semi solids were the most problems. Prescription analysis showed that about 14,2% dispensing prescriptions contain salycilic acid mixed with cream, and 26,5% dispense prescriptions contains salicylic acid mixed with any kinds of drugs or other semi solid forms. Liquid were the minor (7,2%) dispensing prescriptions written by physicians. However problems were still remain caused bycrushing coated tablets and dispensing to the liquid.Keywords : prescription, pharmaceutical stores, dosage forms, Yogyakarta
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/768
10.14499/indonesianjpharm0iss0pp201-207
Indonesian Journal of Pharmacy; Vol 14 No 4, 2003; 201-207
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/768/879
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1505
2019-10-10T01:29:33Z
3:ART
In Vitro Study of the Combination of Doxorubicin, Curcuma xanthorrhiza, Brucea javanica, and Ficus septica as a Potential Novel Therapy for Metastatic Breast Cancer
Sutejo, Ika Rahmawati; Biochemistry Department, Faculty of Medicine, University of Jember, Jalan Kalimantan 37 Jember, Indonesia
Cancer Chemoprevention Research Center, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Putri, Herwandhani; Cancer Chemoprevention Research Center, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Handayani, Sri; Cancer Chemoprevention Research Center, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Research Center for Chemistry, Indonesian Institute of Sciences (LIPI), Gedung 452 Kawasan PUSPIPTEK Serpong, Tangerang Selatan, Banten 15314, Indonesia
Jenie, Riris Istighfari; Department of Pharmaceutical Chemistry Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Cancer Chemoprevention Research Center, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Meiyanto, Edy; Professor of Pharmaceutical chemistry, Faculty of Pharmacy. Universitas Gadjah Mada
C xanthorrhiza, Bjavanica, F septica, antimetastasis, 4T1 cells
Less optimized therapeutic effects constrain the use of doxorubicin as the main agent of chemotherapy for metastatic breast cancer, resistance and side effects. Therefore we need a combination of more than one chemopreventive agent which has different molecular targets to solve that problem. The aims of this study is to prove the inhibitory effect of ethanolic extract of rhizome of Curcuma xanthorrhiza (ECx), fruit of Brucea javanica (EBj), leave of Ficus septica (EFs) and doxorubicin (Dox) alone and its combination on migration and invasion of a highly metastatic 4T1 breast cancer cell line. Cytotoxic activity of single and combination treatment was evaluated by MTT assay, followed by an experiment of apoptosis induction by using flow cytometry. The inhibitory effect on migration was observed by the scratch wound-healing assay. Furthermore, the observation of the activity of matrix metalloproteinase-9 (MMP-9) was analyzed by gelatin zymography. The results showed that ECx, EBj, EFs, and Dox has cytotoxic activity on 4T1 cells with the value of IC50 respectively 49.7±1.53mg/mL, 59.9±1.79mg/mL, 15.2±2.12mg/mL and 1.2±0.23mM. Furthermore, combination of ECx-EBj-Dox and ECx-EBj-EFs revealed synergistic effect on 4T1 cells and decrease cell viability through the induction of apoptosis and necrosis. Based on wound healing assay, 24 hours incubation of this combination inhibited 4T1 cells migration compared to single treatment. Gelatin zymography analysis showed that this combination also inhibited the activity of MMP-9 greater than a single use. Curcuma xanthorrhiza, Brucea javanica, and Ficus septica may have potential to be developed as a combination with or without doxorubicin for metastatic breast cancer treatment.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1505
10.14499/indonesianjpharm30iss1pp15
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 15
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1505/889
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/517
2018-09-05T01:49:55Z
3:ART
ANTIHEPATOTOXIC ACTIVITY OF TWO NEW QUERCETIN DERIVATIVES IN CARBON TETRACHLORIDE INDUCED HEPATOXICITY IN RATS
Khan, Shah Alam; Department of Pharmacy, Oman Medical College, Muscat, Sultanate of Oman PO Box 82, Ruwi, PC 112 Oman
Ahmed, Bahar; Antihepatotoxic Research Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy Jamia Hamdard, New Delhi110062, India
Quercetin, a bioflavonol is widely found in nature and possesses diverse pharmacological properties. A heterocyclic 1,4 dioxane nucleus was incorporated in Quercetin structure to obtain two structural analogues of silybin. The aim of the study was to antihepatotoxic potential of Quercetin derivatives containing 1,4 dioxane heterocyclic ring in Carbon tetrachloride (CCl4) induced hepatotoxicity in female Wistar Albino rats. Two Quercetin derivatives (QD) were synthesized by reported method. QD were administered orally at dose of 10 mg/kg, once daily for 7 days to Wistar Albino rats. A single dose of CCl4(1mL/kg) was used for inducing liver damage. Antihepatotoxic activity was evaluated by measuring levels of total proteins (TP), total albumin (TA), alkaline phosphatase (ALKP) and liver enzymes such as serum glutamate oxaloacetate (SGOT) and serum glutamate pyruvate transaminase (SGPT).QD exhibited potent antihepatotoxic activity with respect to standard drug silybon-70. However, it was observed that the Quercetin derivative having –CH2OH group in the dioxane ring exhibited better activity in comparision to unsubstituted 1,4 dixoane ring derivative.The exact mechanism by which QD protects the liver is unknown however the observed effects could be attributed to presence of 1,4 dioxane ring and due to the significant antioxidant activity of Quercetin flavone. Key words: Antihepatotoxic activity, Quercetin; Silymarin, 1,4 dioxanering, CCl
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2013-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/517
10.14499/indonesianjpharm24iss1pp56-60
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 56-60
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/517/395
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1104
2018-10-23T02:18:57Z
3:ART
Population-Based Approach to Analyze Sparse Sampling Data in Biopharmaceutics and Pharmacokinetics using Monolix and NONMEM
Nugroho, Akhmad Kharis; Department of Pharmaceutics Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta Indonesia
Hakim, Arief Rahman; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada
Hakim, Lukman; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada
model, population, sparse sampling data, Monolix, NONMEM
Although it has been developed since 1972, the implementation of a population-based modeling approach in Indonesia, particularly to analyze biopharmaceutics and pharmacokinetics data is still very limited. This study was aimed to evaluate the performance of Monolix and NONMEM, two of the popular software packages in a population-based modeling approach, to analyze the limited data (sparse sampling data) of the time profiles of the simulated plasma drug concentration of a theoretical compound. and NONMEM were used to model the limited data (40 data points) as a results of the random selection from the 180 point data of simulated plasma drug concentration (Cp) on 20 subjects at 0.25; 0.5; 0.75; 1; 1.5; 3; 6; 12 and 18 hours after per-oral administration of a 100mg of a theoretical compound. Population values of the absorption rate constant (Ka), the elimination rate constant (Kel) and volume of distribution (Vd) were compared to the average Ka, Kel and Vd obtained by the conventional method (two stage approach) using PKSolver on the Cp data of all subjects. The calculation system of a nonlinear mixed effect model in Monolix and NONMEM, successfully describes the sparse data, based on the visual evaluation of the goodness of fit. Comparison of parameter estimates of population values in Monolix and NONMEM are in the range of 94 to 108% of the real values of the rich data analysed by PKSolver. A population-based modeling can adequately analyze limited or sparse data, demonstrating its capability as an important tool in clinical studies, involving patients.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-12-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1104
10.14499/indonesianjpharm28iss4pp205
Indonesian Journal of Pharmacy; Vol 28 No 4, 2017; 205
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1104/853
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1176
2018-01-10T06:56:14Z
3:ART
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/659
2020-01-27T02:53:58Z
3:ART
FORMULATION OF NANOCURCUMIN USING LOW VISCOSITY CHITOSAN POLYMER AND ITS CELLULAR UPTAKE STUDY INTO T47D CELLS
Chabib, Lutfi; Departement of Pharmacy, Faculty of Mathematics and science Universitas Islam
Indonesia 55584, Yogyakarta
Martien, Ronny; Departement of Pharmaceutics, Faculty of
Pharmacy, Universitas Gadjah Mada,
Yogyakarta, Sekip Utara Yogyakarta 55281, Indonesia
Ismail, Hilda; Departement of pharmaceutical Chemistry,
Faculty of Pharmacy, Universitas Gadjah Mada,
Sekip Utara Yogyakarta 55281, Indonesia
Using of curcumin as anti cancer agent is restricted by its low solubility, therefore it has low bioavability. This obstacle can be solved by the development of curcumin nanoparticle. Nanoparticle technology has been started to be developed as an alternative solution to improve drug delivery pofile, especially for the less bio-available chemical. This study was aimed to develope nanocurcumin formulation with low viscosity chitosan as the matrix and to study its ability to be taken into the cells in vitro. Method used in the formulation of nanocurcumin in this study is by ionic gelation followed by freeze drying. Entrapment Efficiency then assayed, and its stability was tested by incubating the formula into artificial intestinal fluid (AIF). Furthermore, its toxicity was evaluated, also its cellular uptake ability into T47D cell line. It was found that the Entrapment Efficiency in acetate buffer at pH 4 is higher than at pH 5. This formula also has a good stability in AIF. For the cellular uptake study through fluorescence microscope, it was found that the complex has an ability to penetrate cellular membrane into the cytosol. The cytotoxicity study tell us that the nanocurcumin is non-toxic to normal cell line. For the characterization of the nanoparticles, the average size of this particle is 269.8 nm, its zeta-potential is +18.63 mV, with spherical particle morphology. From the result ofthis study, it is concluded that formulation of nanocurcumin using low viscosity chitosan polymer as the matrix has a great potential as an alternative for anticancer therapy.Key words: nanoparticle, curcumin, low viscosity chitosan, T47D cell line.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/659
10.14499/indonesianjpharm23iss1pp27-35
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 27-35
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/659/525
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1063
2017-09-11T06:07:08Z
3:ART
SYNTHESIS AND SCREENING OF SOME AZOMETHINES BEARING METHYL SALICYLATE MOIETY FOR ANTIBACTERIAL ACTIVITIES
Baluja, Shipra; Department of Chemistry, Saurashtra University, Rajkot-360005, India
Chanda, Sumitra
In this study, the series of novel azomethines were synthesized from methyl salicylate and their structural determination was done by IR, NMR and mass spectral data. The screening of these synthesized compounds were done against some Gram positive and Gram negative bacteria in DMF and DMSO solvents by agar disc method. It is observed that solvent and compound structure both affect the inhibition. DMF is found to be good solvent in the present study. Amongst all studied compounds, JB-4 showed maximum zone of inhibition against most of the studied bacterial strains while compound JB-2 is least effective. Thus, substitution affects inhibition and methyl salicylate moiety with cinnamaldehyde side chain is most effective. Further, virtual screening of these compounds was done to evaluate molecular properties and bioactive scores of these compounds.Keywords: azomethines, methyl salicylate, antibacterial activity, agar disc method, DMF, DMSO
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-03-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1063
10.14499/indonesianjpharm27iss2pp92
Indonesian Journal of Pharmacy; Vol 27 No 2, 2016; 92
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1063/774
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1468
2019-10-28T01:20:02Z
3:ART
Antibacterial Compound from Aspergillus elegans SweF9 an Endophytic Fungus from Macroalgae Euchema sp.
Mulyani, Hani; 1. Pharmacy Faculty, Pancasila University,Jl. Raya Lenteng Agung Timur No.56-80, Srengseng Sawah, Jagakarsa, Jakarta
2. Indonesian Institute of Sciences (LIPI)
Dewi, Rizna Triana; Indonesian Institute of Sciences (LIPI)
Chaidir, Chaidir; Pharmacy Faculty, Pancasila University,Jl. Raya Lenteng Agung Timur No.56-80, Srengseng Sawah, Jagakarsa, Jakarta
Aspergillus elegans SweF9, antibacteria, (+)-epi-Epoformin.
The antibiotic resistance of bacterial pathogens has become a serious health concern and encouragement to search for novel and efficient antimicrobial metabolites. On the other hand, endophytic fungi have great potential as a natural source for antimicrobial agents. The objective of this study was to isolation antibacterial compound from endophytic fungi of A.elegans SweF9. The fungus was stationarily cultured at 30°C for 12 days in potato malt peptone (PMP) medium, then extracted with ethyl acetate. The antibacterial activities of the extract were evaluated by agar well diffusion method against Gram-poitive (Bacillus subtilis and Staphylococcus aureus) and Gram-negative (Escherichia coli) bacterial strains. The broth extract was able to inhibit the growth of E. coli, S. aureus and B. subtilis with antibacterial activity index compared to streptomycin sulfate were 84.6%, 91.6%, and 90% respectively. The active compound (1) was purified to yield amorphous white and identified using FTIR, NMR, and EI-MS analyses, revealed identified as (+) - epi-Epoformin. The compound showed an antibacterial activity index of E. coli, S. aureus and B. subtilis bacterial were 38%, 45%, and 47%, respectively. Based on these results endophytic fungi A. elegans SweF9 can be used as a new source of potential antibacterial compounds
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Indonesian Institute of Sciences
2019-09-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1468
10.14499/indonesianjpharm30iss3pp217
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 217
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1468/916
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/676
2020-01-27T02:53:16Z
3:ART
DEVELOPMENT AND EVALUATION OF IN SITU GELS OF MOXIFLOXACIN FOR THE TREATMENT OF PERIODONTITIS
Kunche, Hareesh Babu; Department of Pharmaceutics, Sri Adichunchanagiri College of Pharmacy, B.G. Nagara, India
Ahmed, Mohammed Gulzar; Department of Pharmaceutics, Sri Adichunchanagiri College of Pharmacy, B.G. Nagara, India
Rompicharla, Narayana Charyulu; Department of Pharmaceutics, NGSM Institute of Pharmaceutical Sciences, Mangalore , India.
Gel dosage forms are successfully used as drug delivery systems to control drug release and protect the medicaments from a hostile environment. The main objective of the present investigation is to formulate and evaluate in situ gels of moxifloxacin for the treatment of periodontitis using gellan gum and sodium alginate based on the concept of ion activated systems. The system utilizes polymers that exhibit sol-to-gel phase transition due to change in specific physico-chemical parameters. Sol-to-gel transformation occurred in the presence of monovalent/divalent cations. It was found that increase in the concentration of calcium ions produced stronger gels. Formulations were evaluated for gelling capacity, drug content, clarity, viscosity, gel strength, spread ability, microbiological studies and in vitro release. The results shown by the above characterization studies were found to be satisfactory. Experimental part showed that viscosity of sols and gel strength was increased with increase in the concentration of polymers and the sustained release of drug was observed. The formulations were therapeutically efficacious, sterile and provided sustained release of the drug over a period of time. These results demonstrated that the developed system is an alternative to conventional drug delivery systems, provides patient compliance and economical.Key words: in situ gels, moxifloxacin, periodontitis, polymers, Ion activation.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/676
10.14499/indonesianjpharm23iss3pp141-146
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 141-146
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/676/542
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1310
2018-11-02T06:46:53Z
3:ART
Derivatives of 3-(alkylthio)-5-(thiophen-2-ylmethyl)-4H-1,2,4-triazol-4-amines as increasing efficiency substances
Safonov, Andrey; Zaporizhzhia State Medical Univercity
1,2,4-triazole, pharmacological correction of fatigue, method of forced swimming
In today's society, especially in eastern countries such as China, Japan etc., the problem of fatigue and even death at work is acute. One of the solution to this problem is complex therapy with increasing efficiency drugs. Derivatives of 1,2,4-triazole have already proven themselves as potential compounds for pharmacological correction of fatigue. Compounds were synthesized at the Department of toxicological and inorganic chemistry ZSMU. Using method of forced swimming on a group of white nonlinear rats activity of anti-fatigue of the compounds were analyzed. Having analyzed the data of pharmacological correction of fatigue for 3-(alkylthio)-5-(thiophen-2-ylmethyl)-4H-1,2,4-triazol-4-amines, it should be noted that this series of compound do not show anti-fatigue effect. Introduction in molecule 3- (nonylthio)-5-(thiophen-2-ylmethyl)-4H- 1, 2, 4-triazol-4-amine 4-fluorbenzylidene, 4-(dimethylamino) benzylidene, 4- methoxybenzylidene, 2-chloro-6-fluorobenzylidene radicals lead to increase actoprotective effect. The most active compound is N-(2-chloro-6-fluorobenzylidene)-3-(nonylthio)-5-(thiophen- 2- ylmethyl)- 4H- 1, 2, 4- triazol- 4-amine which exceeds the standard (riboxin).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Zaporizhzhia State Medical University
2018-09-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1310
10.14499/indonesianjpharm29iss3pp167
Indonesian Journal of Pharmacy; Vol 29 No 3, 2018; 167
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1310/878
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1156
2017-09-11T06:07:50Z
3:ART
THE IN VITRO ANTIOXIDANT PROPERTIES OF 2-ALKOXYPHENYLCARBAMIC ACID DERIVATIVES CONTAINING A 4´-(SUBSTITUTED PHENYL)PIPERAZIN-1´-YL MOIETY DETERMINED BY THE 2,2´-AZINOBIS(3-ETHYLBENZOTHIAZOLINE-6-SULFONIC ACID) DERIVED RADICAL CATION (ABTS•+) AND FERRIC RE
Malík, Ivan; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Comenius University
Stanzel, Lukáš; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Comenius University
Csöllei, Jozef; Department of Chemical Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences
Čurillová, Jana; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Comenius University
Substituted phenylcarbamates; N-arylpiperazines; antioxidant properties; ABTS•+; FRAP; electronic features; lipophilicity
In an effort to comprehensively characterize an antioxidant profile of 2-alkoxyphenylcarbamic acid-based compounds containing a 4´-(substituted phenyl)piperazin-1´-yl fragment, they were in vitro screened in the 2,2´-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) derived radical cation (ABTS•+) and ferric reducing antioxidant power (FRAP) assay using the UV/VIS spectrophotometry. The ABTS•+ scavenging (reducing) potential of 1-[3-(2-methoxyphenylcarbamoyl)oxy-2-hydroxypropyl]-4-(4-fluorophenyl)piperazin-1-ium chloride was found to be the most promising and it was comparable to the efficiency of the carvedilol reference drug. Moreover, that 4´-fluoro group-containing compound was regarded as more active than the atenolol standard. When testing the molecules´ power to reduce the ferric 2,4,6-tris (2-pyridyl)-s-triazine complex [Fe(III)(TPTZ)2]3+, the most prospective was 1-[3-(2-ethoxyphenylcarbamoyl)oxy-2-hydroxypropyl]-4-(4-fluorophenyl)piperazin-1-ium chloride. On the other hand, its Fe3+ reducing power was lower compared to both standards carvedilol and atenolol. The study discussed structure–antioxidant properties relationships considering electronic, steric and lipophilic features.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-03-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1156
10.14499/indonesianjpharm28iss1pp1
Indonesian Journal of Pharmacy; Vol 28 No 1, 2017; 1
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1156/805
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1410
2022-10-26T01:05:53Z
3:ART
In Vitro Study: Effect of Cobalt(II) Chloride Against Dengue Virus Type 1 in Vero Cells
Sucipto, Teguh Hari; Institute of Tropical Disease, Universitas Airlangga
Untoro, Yovilianda Maulitiva; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga
Setyawati, Harsasi; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga
Maharani, Anisa; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga
Sholihah, Novia Faridatus; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga
Churrotin, Siti; Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga
Amarullah, Ilham Harlan; Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga
Soegijanto, Soegeng; Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga
Cobalt(II) chloride, DENV-1, and Antiviral activity.
Dengue virus (DENV) serotypes DENV-1 to DENV-4 are enveloped viruses that belong to the genus Flavivirus of the Flaviviridae. Dengue vaccine or antiviral has not yet been clinically approved for humans, even though there have been great efforts toward this end. Antiviral activity against DENV is needed to develop to be an alternative drug for DENV virus. Cobalt(II) chloride have been used in the treatment and prevention of diseases of humans since ancient times. The aim of this study is to investigate the antiviral effects and Cytotoxicity of Cobalt(II) chloride. This compound was further investigated for its inhibitory effect on the replication of DENV-1 in Vero cells. Antiviral activity and Cytotoxicity measured by WST-1 assay. The IC50 value of the Cobalt(II) chloride for DENV-1 was 0.38 μg/ml. The cytotoxicity of Cobalt(II) chloride to Vero cell suggest that the CC50 value was 2.91 µg/ml The results of this study demonstrate the anti-dengue serotype 1 inhibitory activity of Cobalt(II) chloride was a high toxic compound.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-01-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1410
10.14499/indonesianjpharm30iss4pp316
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 316
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1410/931
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/687
2020-01-27T02:53:31Z
3:ART
ANTHELMINTIC ACTIVITY OF Cuminum cyminum, FOENICULUM VULGARE AND Eugenia caryophyllusAGAINST Pheritima posthuma
Kumar, Satish; Department of Pharmacognosy, Gautham
college of Pharmacy, Bangalore, Karnataka,
560032 India
Goyal, Kamal Kumar; Department of Pharmacognosy, Sree Balaji college of Pharmacy,Jaipur, Rajasthan, 302 004India.
Bilal, Syed; Department of Pharmacognosy, Gautham
college of Pharmacy, Bangalore, Karnataka,
560032 India
Ethanol extract of fruit of Cuminum cyminum (EECC), aqueous extract of fruit of Cuminum cyminum (AECC), Ethanol extract of fruit of Foeniculum vulgare (EEFV), aqueous extract of fruit of Foeniculum vulgare (AEFV) and Ethanol extract of flowering bud of Eugenia caryophyllus( EEEC), aqueous extract of flowering bud of Eugenia caryophyllus( AEEC) were taken up for in vitro studies on the Anthelmintic activity against Pheritima posthuma. Different concentrations (25, 50, 100, 200mg/mL) were used for the activity is compared with the reference standard Albendazole at concentration 20mg/mL and normal saline( 0.9% NaCl) was used in control treatment. The results were expressed in terms of time in minutes to report the time of paralysis and time of death of the earth worms. The results obtained from study indicate towards anthelmintic activity, supporting folk used of all three plants compared with reference standard. The result established that EEEC and AEEC is more potent as compared with EECC, AECC and EEFV, AEFV.Key words: Anthelmintic activity, Cuminum cyminum, Eugenia caryophyllus, Foeniculum vulgare, Pheritima posthuma.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/687
10.14499/indonesianjpharm23iss4pp225-230
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 225-230
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/687/553
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1334
2019-10-10T01:29:33Z
3:ART
Profile of Biofilm-Producing Staphylococcus epidermidis from Intravenous Catheter Colonisation at Prof. Dr. Margono Soekarjo Hospital Purwokerto
Anjarwati, Dwi Utami; Jenderal Soedirman University
Siswandari, Wahyu; Dept of Pathology Clinic, Medical Faculty of Jenderal Soedirman University-Margono Soekarjo Hospital, Dr Gumbreg Nomor I, Medical Street, Mersi, Purwokerto, 53112
Peramiarti, IDSAP; Dept of Microbiology, Medical Faculty of Jenderal Soedirman University, Dr Gumbreg Nomor I, Medical Street, Mersi, Purwokerto, 53112
Biofilm, Intravenous catheter, colonization, Staphylococcus epidermidis
Biofilm- producing Staphylococcus epidermidis has evolved to be a significant human pathogen, particularly in the use of medical devices such as an intravenous catheter. Furthermore, biofilm-producing bacteria 10-1000 fold less susceptible to several antimicrobial agents than free-bacteria. This simple survey aimed to describe the profile of biofilm-producing S. epidermidis from intravenous catheter colonization of some patients in surgical and internal medicine wards at the hospital Margono Soekarjo, Purwokerto, and the antibiotics resistance pattern. A vitek® 2 compact (Enseval Medika Prima) was performed to identify the bacterial species and to examine the 73 antibiotics for understanding the resistance pattern automatically. Microtiter plate biofilm assay with crystal violet staining was performed to measure biofilm optical density (OD) for analyzing the biofilm production capabilities. A scanning electron microscopy (SEM) was done to compare the thickness of ultrastructure of biofilm-producing S. epidermidis visually. The present study found that 2 of 8 Gram-positive bacteria (25%) were biofilm-producing S. epidermidis. One of S. epidermidis was moderate whereas the other was high biofilm-producing bacteria. Images of SEM showed that a high biofilm-producing S. epidermidis has a thicker ultrastructure of biofilm than the moderate biofilm-producing, whereas a control, the weak biofilm-producing S. epidermidis ATCC 12228 has the least biofilm. Both of S. epidermidis strains were sensitive to Gentamicin, Moxifloxacin, Quinupristin/Dalfopristin, Linezolid, Vancomycin, Doxycycline, Minocycline, Tetracycline, Tigecycline, and Nitrofurantoin. Furthermore, both S. epidermidis strains were resistant to the other (63) antibiotics. In conclusion, two strains of S. epidermidis in this study have different capabilities to form the biofilm which were showed that high biofilm-producing strain was thicker than moderate biofilm-producing strain by scanning electron microscopy. However, both of them were resistant to the same number of antibiotics.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Prof. dr. Margono Soekarjo Hospital, Purwokerto
Research and higher education ministry, Indonesia
2019-03-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1334
10.14499/indonesianjpharm30iss1pp1
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 1
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1334/887
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/511
2018-09-05T01:49:54Z
3:ART
DESIGN, DEVELOPMENT AND IN VITRO EVALUATION OF CAFFEINE LOADED NATURAL GUM MATRIX TABLET
Jain, Nidhi; Dept. of PharmaceuticsSchool of Pharmacy, Bharat Institute of Technology., By-Pass Road, Partapur, Meerut250103, Uttar Pradesh, India
Kumar, Divya; Dept. of PharmaceuticsSchool of Pharmacy, Bharat Institute of Technology., By-Pass Road, Partapur, Meerut250103, Uttar Pradesh, India
Gulati, Neha; Dept. of PharmaceuticsSchool of Pharmacy, Bharat Institute of Technology., By-Pass Road, Partapur, Meerut250103, Uttar Pradesh, India
Nagaich, Upendra; Dept. of PharmaceuticsSchool of Pharmacy, Bharat Institute of Technology., By-Pass Road, Partapur, Meerut250103, Uttar Pradesh, India
The present research work was carried out to develop sustained release tablets of caffeine using natural matrix former (tragacanth) and different filling polymers like hydroxyl propyl methyl cellulose (HPMC K15M) and ethyl cellulose (EC). Caffeine was used as model drug. The polymers and tragacanth gum were incorporated in varying ratios into a matrix system using wet granulation technique. All the lubricated formulations were compressed into tablets and evaluated for various physicochemical properties such as thickness, hardness, friability, weight variation, drug content and in vitro drug release studies. From the investigation it was observed that increase in the amount of gum tragacanth (from F1 to F5) led to reduced friability, increased hardness and retarded drug release. Different filling polymers also sustained the drug release. The in vitro drug release data were tted in various release kinetics models to understand th mechanism of drug release. All solid matrix formulations were found to follow Higuchi kinetics, indicating the diffusional release of drug from the matrix type system. The Formulation F5 containing highest amount of gum tragacanth have shown promising results. The findings of the current investigation clearly indicate the potential of tragacanth gum to be used as release retardant and natural matrix material in sustained release formulations. Key words: Sustained release, Matrix tablets, Tragacanth, Caffeine.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2013-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/511
10.14499/indonesianjpharm24iss1pp30-34
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 30-34
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/511/389
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1085
2017-09-11T06:09:19Z
3:ART
OPTIMIZING STRUCTURE-BASED VIRTUAL SCREENING PROTOCOL TO IDENTIFY PHYTOCHEMICALS AS CYCLOOXYGENASE-2 INHIBITORS
Istyastono, Enade Perdana; Division of Drug Design and Discovery, Faculty of Pharmacy, Sanata Dharma University
Center for Environmental Studies Sanata Dharma University (CESSDU)
Structure-based virtual screening (SBVS); phytochemical; cyclooxygenase-2 (COX-2)
By employing Databases of Useful Decoys (DUD) and its enhanced version (DUD-E), several attempts to construct validated Structure-based Virtual Screening (SBVS) protocols to identify cyclooxygenase-2 (COX-2) inhibitors have been performed. Both databases tagged active COX-2 inhibitors for compounds with IC50 values < 1mM. In the search for phytochemicals as natural COX-2 inhibitors, however, most of their IC50 values are in the micromolar range, which will likely be identified as non-inhibitors for COX-2 by the available SBVS protocols. In this article, validation of an SBVS protocol by adding marginal active COX-2 inhibitors from DUD-E as active compounds is presented. Binary quantitative-structure activity relationship analysis by using recursive partition and regression tree method was performed subsequently to optimize the predictive ability of the protocol. The enrichment factor and the F-measure values of the optimized protocol could reach 44.78 and 0.47, respectively. The optimized protocol could identify 1 out of 9 phytochemicals as COX-2 inhibitors.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1085
10.14499/indonesianjpharm27iss3pp163
Indonesian Journal of Pharmacy; Vol 27 No 3, 2016; 163
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1085/780
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1493
2021-01-07T01:00:39Z
3:ART
THE EFFECT OF GIVING DELTAMETHRIN AND CYPERMETHRIN INSECTICIDES ON THE LEVEL OF INTRATESTICULAR TESTOSTERONE IN MALE WISTAR STRAIN RATS
Triutomo, Devyanto Hadi; Faculty of Pharmacy UGM
Puspitasari, Ika; Faculty of Pharmacy, Universitas Gadjah Mada
Susidarti, Ratna Asmah; Faculty of Pharmacy, UGM
cypermethrin, deltamethrin, intratesticular testosterone
The use of deltamethrin and cypermethrin insecticides to control pests has been implemented in Indonesia. Irregular and excessive use of insecticides can have toxic effects on the male reproduction system. Deltamethrin and cypermethrin insecticides can inhibit androgen biosynthesis and disrupt the growth of sperm. Therefore, this research is conducted to find out the effect of deltamethrin and cypermethrin insecticides toward the level of intratesticular testosterone in male Wistar strain rats. This research is conducted in vivo using male Wistar strain rats. Nine rats are divided into three groups of treatment, namely control group, deltamethrin group with 0.26 mg/kg of weight, and cypermethrin group with 0.26 mg/kg of weight. Treatment is conducted every day for 15 days orally, and then the rats are dissected to take their testicles. Testicles are chopped and their intratesticular homogenates are taken by adding the medium of DMEM and collagenase (0.25 mg/mL). The measurement of the level of testosterone is conducted by using Electrochemiluminescence immunoassay (ECLIA) method. Data on the change of the increase of body weight, organ weight, and the level of testosterone is analyzed statistically using the one-way ANOVA test. The research result shows that giving 0.26 mg/kg of weight of deltamethrin and 0.26 mg/kg of weight of cypermethrin does not affect the increase in body weight, relative weight of the reproductive organ, and the level of intratesticular testosterone of male Wistar strain rats.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-08-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1493
10.14499/indonesianjpharm31iss2pp116
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 116
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1493/947
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1022
2017-09-11T04:58:23Z
3:ART
FORMULATION AND EVALUATION OF ORODISPERSIBLE TABLETS OF GRANISETRON HYDROCHLORIDE USING PLANTAGO OVATE AS NATURAL SUPERDISINTEGRANTS
Sahoo, Chinmaya Keshari; Osmania University College of Technology, Osmania University, Hyderabad,Telangana-500007,India
Sahoo, Nalini Kanta; MNR College of Pharmacy, Fasalwadi, Sangareddy, Medak, Telangana, 502294,India
Sahu, Madushima; MNR College of Pharmacy, Fasalwadi, Sangareddy, Medak, Telangana, 502294,India
., V.Alagarsamy; MNR College of Pharmacy, Fasalwadi, Sangareddy, Medak, Telangana, 502294,India
Moharana, Alok Kumar; Omega College of Pharmacy, edulabad, ghatkesar, Ranga Reddy Dist -500388,India
Sarangi, Deepak Kumar; MNR College of Pharmacy, Fasalwadi, Sangareddy, Medak, Telangana, 502294,India
Satyanarayana, Kokkula; Princeton College of Pharmacy, Princeton Group of Educational Institutions,Ghatkesar,R.R Dist. Telengana-500088,India
The main objective of the study was to develop orodispersible tablets of Granisetron hydrochloride, a selective 5-HT3 receptor antagonist (an antivomiting agent) for improving patient compliance, especially those of paediatric & geriatric categories with difficulties in swallowing. In the wet granulation method orodispersible (ORD) tablets were prepared using natural super disintegrants plantago ovate.The prepared batches of tablets were evaluated for weight variation, hardness, friability, wetting time, in vitro dispersion time, drug content and in vitro dissolution studies. The tablet formulation batch F4 was considered as the overall best formulation (with an in vitro drug release study of 99.11%).Short term stability studies (at 40±2ºC/75±5% RH) on the best formulation indicated that there no significant changes in drug content. From the Fourier Transform Infrared (FTIR) spectroscopy study indicated that there are no drug excipient interactions. Key words: Granisetron hydrochloride, Orodispersible tablets, FTIR spectroscopy, in vitro drug release study.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1022
10.14499/indonesianjpharm27iss1pp35
Indonesian Journal of Pharmacy; Vol 27 No 1, 2016; 35
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1022/766
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1031
2017-09-11T05:00:54Z
3:ART
PHYSICAL CHARACTERIZATION OF IBUPROFEN-STEARIC ACID BINARY MIXTURE DUE TO COMPRESSION FORCE
Setyawan, Dwi; Dept of Pharmaceutics, Faculty of Pharmacy, Airlangga University, Darmawangsa Dalam Surabaya-Indonesia
Isadiartuti, Dewi; Dept of Pharmaceutics, Faculty of Pharmacy, Airlangga University, Darmawangsa Dalam Surabaya-Indonesia
Betari, Sita Desti; Dept of Pharmaceutics, Faculty of Pharmacy, Airlangga University, Darmawangsa Dalam Surabaya-Indonesia
Paramita, Diajeng Putri; Dept of Pharmaceutics, Faculty of Pharmacy, Airlangga University, Darmawangsa Dalam Surabaya-Indonesia
Aim of this research is to determine physical characteristic of ibuprofen-stearic acid due to compression force. Binary mixture of ibuprofen-stearic acid with weight ratio of 4:6, 5:5, and 6:4 was compressed under various compression force using hydraulic press with 13mm diameter flat punch.Identification of solid state interaction between these two components was performed by Hot Stage Microscopy (HSM). Physical characterization has been studied byDifferential Thermal Analysis (DTA), X-ray Powder Diffraction (XRPD), Scanning Electron Microscopy (SEM), and (Fourier Transform Infrared (FT-IR) spectroscopy. According to hardness measurement, 4:6 weight ratio has the highest tensile strength on 170.6N/cm2 under 19.93kN compression force. Interaction identification by HSM showed single blank line that indicates eutectic formation. Thermal analysis of DTA also revealed eutectic formation upon compression whichthe endotermic peak of ratio of 4:6has the lowest melting temperature of 53.2°C. X-ray diffraction of three peaksgenerally showed that peak intensity decreases as compression force increases, but at particular point it begins to increase again. Sintering phenomenon at the surface of compressed tablet was observed from SEM analysis. FT-IR study confirms the formation of simple eutectic. Key words: ibuprofen, stearic acid, binary mixture, compression force, physical characterization
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1031
10.14499/indonesianjpharm27iss1pp28
Indonesian Journal of Pharmacy; Vol 27 No 1, 2016; 28
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1031/765
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1519
2019-10-28T01:20:02Z
3:ART
A Study of Psychoactive Medicines and Risk of Falls Among Indonesian Elderly Patients
Rahmawati, Fita; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia 55281
Mustafidah, Nasikhatul; Postgraduate Magister of Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia 55281
Department of Pharmacy RSUD. Dr. Soedono Madiun
Pramantara, I Dewa Putu; Department of Geriatric, RSUP Dr. Sardjito Hospital, Yogyakarta, Indonesia 55281
Wahab, Izyan Abdul; Faculty of Pharmacy, Cyberjaya University College of Medical Sciences, Persiaran Bestari, Cyber 11, 63000 Cyberjaya, Selangor, Malaysia
Psychoactive medicine, Fall risk, Elderly
One of the causes of injury to the elderly is due to falls. Falling can be prevented by identifying and controlling risk factors. One risk factor that can be controlled is the use of fall risk medicines including psychoactive. This study aims to identify the association between the use of psychoactive medicine and its characteristic with the risk of falls among the elderly in Indonesia. The study utilized a case-control study design for a total number of 414 elderly patients, during October until December 2018. Cases were elderly aged 60 years or above with a high risk of falling assessed using the Morse Fall Scale (MFS≥45). Each case was matched with up to two randomly selected controls of the same age who are classified as low to moderate risk of falling (MFS<45). The use of psychoactive medicines was screened from a history of drug use for the past six months. Psychoactive medicine-fall risk associations were estimated via logistic regression. There were 138 cases and 276 controls. The median age of subjects was 66 years old and 54.83% was a woman. Elderly with a high risk of falling had higher psychoactive medicines use when compare with controls (31.16 % vs 21.38 %, p< 0.05). After adjusting for potential confounders, the use of psychoactive medicines was significantly associated with higher fall risk in elderly patients (OR 1.79 95% CI 1.10-2.90). Only the duration of psychoactive medication use over 90 days was significantly associated with a high risk of falling (AOR 3.65 95% CI 1.46-9.14). In elderly patients, the continued use of psychoactive medicines increased the risk of fall. Prescribers need to weigh risk and benefit from the use of psychoactive medicines in the elderly to prevent future fall.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Faculty of Pharmacy Universitas Gadjah Mada
2019-07-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1519
10.14499/indonesianjpharm30iss3pp233
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 233
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1519/907
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/670
2020-01-27T02:51:49Z
3:ART
BIOCHEMICAL CHANGES OF CERTAIN PLANT SPECIES DUE TO VARIOUS DRYING TREATMENTS
Iqbal, Pandith Javid; Department of Botany, Govt. College, Baramulla 193101,
Kashmir, India
Phytochemical analysis of the Indian medicinal plants to identify the therapeutically active chemical compounds in them and clinically testing for their efficacy and risks is an inescapable procedure, to provide new sources of natural products to the pharmaceutical industry. Therefore, the phytochemical screening studies should be concentrated on the hitherto unexplored medicinal plants for their medicinal values. In the current study the leaves of different medicinal plants like Achyranthus aspera, Acalypha indica, Euphorbia hirta, Lindenbergia indica, Parthenium hysterophorus and Pesistrophe bicalyculata were taken for the chlorophyll and carotenoid estimation by the method of Jayaraman (1981). The leaves of the following plants were allowed to dry in an oven at different temperatures, time period and freeze drying. Oven drying was done at 50±10C for 9 hours and at 70±1ºC for 5 hours. Results obtained revealed that both the drying treatments affect/decrease the chlorophyll content of all the six plants. The maximum effect was seen in Euphorbia hirta (93.97%) for oven drying at 50±1ºC for 9 hours. The minimum effect was observed in Parthenium hysterophorus (21.68%) for oven drying at 70±1ºC for 5 hours and Achyranthus aspera (11.56%) for freeze drying. The leaf preparations treated by oven drying was found to have lower chlorophyll and carotenoid content as compared to freeze drying which in turn has lower chlorophyll content as compared to fresh extracts.Key words: Chlorophyll content, Drying treatments, and leaf extract.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/670
10.14499/indonesianjpharm23iss2pp110-114
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 110-114
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/670/536
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1329
2018-10-23T02:19:33Z
3:ART
Effects of Probiotics and Vitamin B Supplementation on IFN-γ and IL-12 Levels During Intensive Phase Treatment of Tuberculosis
Suprapti, Budi; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Suharjono, Suharjono; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Raising, Rahmawati; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Yulistiani, Yulistiani; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Izzah, Zamrotul; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Nilamsari, Wenny Putri; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Wulaningrum, Prastuti Asta; Department of Pulmonology and Respiratory Medicine, Universitas Airlangga Teaching Hospital, Surabaya
Bachtiar, Arief; Department of Pulmonology and Respiratory Medicine, Universitas Airlangga Teaching Hospital, Surabaya
Interleukin-12, Interferon-γ, Probiotics, Tuberculosis, Vitamin B
Tuberculosis is an acute infectious disease that primarily affects the lungs. Probiotics supplementation can increase the number and activity of NK cell in peripheral blood by modulation of IL-12, thus increasing IFN-γ production by Th1 response. Vitamin B1 acts on macrophages and affects neutrophil motility. Vitamin B6 is associated with the release of cytokines and the responsiveness of NK cells, while vitamin B12 affects to lymphocytes, Tcell proliferation, CD4+ ratios, and NK cell activity. To analyze the effects of probiotics and vitamin B1, B6, B12 supplementation on IFN-γ and IL-12 levels during intensive phase of antituberculosis treatment. The study was pre-post test randomised control by time series. The control group was TB patients with standard therapy of antituberculosis and vitamin B6, while the intervention group was TB patients receiving therapy plus once daily probiotics and vitamin B1, B6, B12supplementation during the intensive phase. Blood samples were withdrawn at baseline, one month, and two months after therapy to measure plasma IFN-γ and IL-12 levels using the ELISA method. Twenty two patients were divided equally into two groups. There was a tendency to greater increase of IFN-γ in the first month of the intervention group, followed by a significant decline after two-month therapy (p < 0.05). In both groups there was a rise in IL-12 levels after one month followed by a decrease in the second month (p > 0.05). However, the percentage was higher in the supplementation group. Adding probiotics and vitamins B1, B6, B12 could improve immune response through IL-12 and IFN-γ modulation during intensive phase therapy.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-06-22
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1329
10.14499/indonesianjpharm29iss2pp80
Indonesian Journal of Pharmacy; Vol 29 No 2, 2018; 80
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1329/868
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/258
2019-10-09T07:05:35Z
3:ART
IN VITRO EFFECT OF Chloroprocta SP. MAGGOTS SECRETION ON Staphylococcus epidermidis BIOFILM AND THE EXPRESSION LEVEL icaA OF GENE
Anjarwati, Dwi Utami; Lab. of Microbiology ,
Faculty Medicine and
Health Science. Jendral
Soedirman University.
Jl. Dr. Suparno-Purwokerto,
53122
Setiawati, Setiawati; Lab. of Farmacology Faculty
Medicine and Health Science
of Jendral Soedirman
University. Jl. Dr. SuparnoPurwokerto, 53122
Mujahidah, Mujahidah; Dept. of Microbiology Faculty of Medicine of
Diponegoro University – Kariadi Hospital. Jl. Doktor Soetomo No.14, 50244, Indonesia
Hapsari, Rebriarina; Dept. of Microbiology Faculty of Medicine of
Diponegoro University – Kariadi Hospital. Jl. Doktor Soetomo No.14, 50244, Indonesia
Nuryastuti, Titik; Dept. of Microbiology Faculty Medicine. Gadjah
Mada University. Yogyakarta, Sekip Utara
55281, Indonesia
Biofilm formation and the expression of icaA gene can be induced by environment conditions that are potentially toxic for bacterial cells. The effect of green flies maggots secretion to biofilm was studied some years ago to investigate in vitro effect of secretion of Chloroprocta sp. maggots on the formation of Staphylococcus epidermidis biofilm (phenotype) and the expression level of icaA gene (genotype) for indicating its mechanism on bacterial biofilm eradication. Microtiter plate biofilm assay was used to measure the effect of Chloroprocta sp. maggots secretion at various concentration on S. epidermidis biofilm. The expression level of icaA gene was performed by Real TimePCR using lightcycler method. The biofilm susceptibility test was done against maggots excretion/secretion using MTT assay. Whereas planktonic susceptibility testing was carried out using Kirby Bauer method. In the presence of maggots secretion at low concentration (5%), biofilm formation of S. epidermidis 734 was induced. In contrast, the expression level of icaA gene in production of maggots excretion/ secretion at concentration of 5% was lower than that of without maggots secretion (1/2 Fold). Eradication of bacterial biofilm was demonstrated after 48h incubation (MD=-0,011;P<0,05), but planktonic cell. In vitro difference effect of the Chloroprocta sp. maggots secretion at low concentration to phenotype and genotype of S. epidermidis biofilm showed that the possibility of maggots secretion ability to eradicate bacterial biofilm was not mainly due to the expression level of icaA gene.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2014-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/258
10.14499/indonesianjpharm25iss2pp76
Indonesian Journal of Pharmacy; Vol 25 No 2, 2014; 76
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/258/142
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1403
2022-10-26T01:05:53Z
3:ART
The capability of Several Population-based Approach Software to Analyze Sparse Drug Plasma Concentration Data after Intra-Venous Bolus Injection
Nugroho, Akhmad Kharis; Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta Indonesia
Hakim, Lukman; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, INDONESIA
Monolix, NONMEM, WinBUGS-PKBUGS, sparse data, therapeutic drug monitoring
Monolix, NONMEM, and WinBUGS-PKBUGS are among available software package for population-based modeling. The sparse condition of drug plasma concentration versus time (Cp-time) data is prevalent in clinically based studies involving patients. It is not ethical in this case, to collect a many and large volumes of blood samples. This study was aimed to simulate the capability of Monolix, NONMEM, and WinBUGS-PKBUGS to analyze very sparse Cp-time data after an intravenous bolus drug administration and to estimate the minimum number of Cp-time data required for an adequate analysis. Data of Cp-time were obtained based on simulation using the pharmacokinetic one-compartment open model following an intravenous bolus administration of 50 mg of a hypothetical drug. In this respect, six random values of k (rate constant of elimination) and Vd (volume of distribution) with mean and standard deviation values of 0.3 ±0.1 per hour and 30 ± 10 L, respectively, were used to create simulated Cp-time data of 6 subjects. Simulated Cp-time data in each subject were randomly ranked to choose data based on the intended number of samples in each subject. Several sparse Cp-time data scenarios, starting from a very limited state, i.e., with a total of 6 Cp-time data (1 datum per subject) to a rich situation with 48 Cp data (8 data per subject), were examined.The goodness of fit evaluations, as well as the similarity of individual values of k and Vd to the respective real values (p>0.05), indicate that nonlinear-mixed-effect-model using Monolix, NONMEM and WinBUGS-PKBUGS can appropriately describe sparse Cp-time data even with only 2 data per subject. This fact is an important finding to support the demand of analytical tool for a limited number of Cp-time data such as obtained in therapeutic drug monitoring event.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Hibah Penelitian Dasar Unggulan Perguruan Tinggi, a research grant scheme provided by the Ministry of Research, Technology and Higher Education, the Republic of Indonesia, year 2018.
2019-10-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1403
10.14499/indonesianjpharm30iss4pp293
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 293
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1403/923
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/682
2020-01-27T02:53:16Z
3:ART
ATTEMPTED SYNTHESIS OF BIS-SPIROEPOXIDE DITHIANEDIOXIDE
Ritmaleni, Ritmaleni; Dep. Pharmacy Chemistry Faculty of Pharmacy , Universitas Gadjah Mada, Sekip Utara, Yogjakarta, 55281, Indonesia
Aggarwal, Varinder K.; School of Chemistry, University of Bristol, Cantocks Close, Bristol, BS8 1TS, UK
The bis-spiroepoxide dithiane dioxide has been attempted to be synthesised as its role as an intermediate in the synthesis of diaminopimelic acid (DAP). The first method was carried out by reacting the 3-dithiane-2-diethylphosphonate 4 with an aqueous solution of the commercially available glutaraldehide resulting the bis-ketene dithiane dioxide 5. The second alternative method was involving the ozonolysis of cyclopentene 7 in the synthesis of bis-ketene dithiane dioxide 5 in four step reactions which gave moderate to good yield. Unfortunately, epoxidation process for the bis-ketene dithiane dioxide 5 was still unsuccess yet.Key words: synthesis, diaminopimelic acid, bis-spiroepoxide dithiane dioxide
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/682
10.14499/indonesianjpharm23iss3pp188-192
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 188-192
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/682/548
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1378
2019-10-03T03:16:50Z
3:ART
Synthesis and Molecular Docking Studies of N’-benzoylsalicylhydrazide derivatives as antituberculosis through InHA enzym inhibition
Santosa, Harry; University of Surabaya
Putra, Galih Satrio; University of Surabaya
Yuniarta, Tegar Achsendo; University of Surabaya
Budiati, Tutuk; Airlangga University
Antituberculosis, Benzoylsalicylhydrazide, Methyl Salicylate, InHA, Microwave synthesis, Molecular docking
The specific aims of this study is to synthesize and to study the possible mechanism of N’-benzoylsalicylhydrazide derivatives as an antituberculosis agent through InhA (Enoyl acyl carrier protein reductase) inhibition using in silico method. Five analogues of N’-benzoylsalicylhydrazide were synthesized using microwave irradiation from methyl salicylate as starting material, which yielded 80-90% product on average. This indicates a considerable improvement in terms of effectivity and efficiency, compared to the more conventional method using reflux condition. Character-ization of the compounds were subsequently carried out by UV, FTIR, 1H-NMR, 13C-NMR spectroscopy, which confirmed that the compounds had been successfully synthesized. Ultimately, molecular docking was performed using Molegro Virtual Docker (MVD) on the active site of InhA enzyme to predict the activity of the compounds. The results showed that all compounds performed comparatively well against N-(4-Methylbenzoyl)-4-benzylpiperidine as the native ligand and also yielded lower docking score than isoniazide (INH). From this study it can be concluded that N’-benzoylsalicylhydrazide derivatives could be synthesized using microwave irradiation with good product yield and all of the synthesized analogues are suggested to possess antituberculosis activity via InhA enzyme inhibition. In vitro activity will have to be determined in the future to validate whether N’-benzoylsalicylhydrazide derivatives perform well as a potential antituberculosis agent.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-11-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1378
10.14499/indonesianjpharm29iss4pp198
Indonesian Journal of Pharmacy; Vol 29 No 4, 2018; 198
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1378/884
Copyright (c) 2018 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/506
2018-09-05T01:49:54Z
3:ART
SUPER POROUS HYDROGELS: A RECENT ADVANCEMENT IN GASTRORETENTIVE DRUG DELIVERY SYSTEM
Mayur, Chordiya; Dept of Pharmaceutics, C.L. Baid Metha College of Pharmacy, Thorapakkam, Chennai, 600096., India. Dept of Pharmaceutics,K.K.
K, Senthilkumaran; Dept of Pharmaceutics,K.K. College of Pharmacy, Mangadu, Chennai, 600096, India.
Hemant, Gangurde; Dept of Pharmaceutics, Nandha Collegeof Pharmacy, Erode, 638052 India.
Super porous hydrogels (SPHs) were originally developed as a controlled drug delivery system to retain drugs in gastic medium. Super porous hydrogels (SPHs) are recent advancement in gastro retentive drug delivery system (GRDDS) which also includes intragastric floating system (low density system), mucoadhesive system, high density system and swellable system. Super porous hydrogels should instantly swell in the stomach and maintain their integrity in the harsh environment and release the pharmaceutical active ingredient. SPH swell fast, within minutes, the fast swelling property is based on water absorption through open porous structure by capillary force. This review discusses about the GRDDS, difference between gels and hydrogels and comparison between SAP vs. SPH. It also includes types of SPH, different generations, general synthesis, methods of preparations, gastric emptying, advantages, characterization, applications and salient features of SPH. Key words: Superabsorbent polymers (SAP), Super porous hydrogel (SPH), Gastro retentive drug delivery system
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2013-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/506
10.14499/indonesianjpharm24iss1pp1-13
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 1-13
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/506/384
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1203
2018-01-09T08:39:22Z
3:ART
Chemical composition of rhizome oleoresin and anti-inflammatory, antinociceptive and antipyretic activity of oleoresins of Alpinia allughas Roscoe. from tarai region of Uttarakhand
Kumar, Ravendra; Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar, U.S. Nagar, Uttarakhand, India
Sethi, Sonali; Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar, U.S. Nagar, Uttarakhand, India
Prakash, Om; Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar, U.S. Nagar, Uttarakhand, India
Pant, Anil Kumar; Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar, U.S. Nagar, Uttarakhand, India
Kumar, Mahesh; Department of Vet. Epidemiology and Preventive Medicine, College of Veterinary & Animal Sciences. G.B. Pant University of Agriculture & Technology, Pantnagar, U.S. Nagar, Uttarakhand, India
Isidorov, Valery A.; Institute of Chemistry, Białystok University, Division of Environmental Chemistry, UL. Hurtowa 1,15-399, Białystok, Poland
Szczepaniak, Lech; Institute of Chemistry, Białystok University, Division of Environmental Chemistry, UL. Hurtowa 1,15-399, Białystok, Poland
ABSTRACT The investigation of volatile constituents of the rhizome oleoresin of Alpinia allughas Roscoe. growing in tarai region of Kumaun hills, Uttarakhand, revealed the presence of 55 terpenoids. The major constituents identified in the rhizome oleoresin were α-eudesmol (21.3%), β-selinene (13.9%), valencene (9.6%), borneol (7.1%), α-humulene (5.3%) and 7-epi-α-selinene (5.2%). Other minor constituents identified were (E)-β-caryophyllene (3.8%), (6 E)-nerolidol (3.5%), (E,E)-farnesol (3.2%), caryophyllene oxide (2.5%), humulene oxide (2.7%), bornyl acetate (1.9%), coranarin- E (1.8%), linalool (1.8%) and α-terpineol (1.6%). The total identified constituents contribute 97.0% of the oleoresin. The rhizomes oleoresins exhibited significant antinociceptive activity with 34.79% inhibition at 50 mg/kg body weight and 43.24% at 100 mg/kg body wt. compare to standard drug ibuprofen (40 mg/kg body wt.), it also showed antipyretic activity in dose dependent manner with temperature reduction 77.57±5.88% at 50 mg/kg body wt. and 98.95±3.95% at 100 mg/kg body wt. after 3 hours. Oleoresin also showed 29.23% inhibition in carrageenin-induced paw edema at 50 mg/kg body wt. and 39.92% inhibition at 100mg/kg body wt. in compare to ibuprofen 40.06% at 40 mg/kg body weight.Keywords Alpinia allughas Roscoe.; Zingiberaceae; α-eudesmol; β-selinene; anti-inflammatory; antinociceptive; antipyretic activity; oleoresins
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
UGC, NEW DELHI, INDIA
2017-08-16
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1203
10.14499/indonesianjpharm28iss3pp136
Indonesian Journal of Pharmacy; Vol 28 No 3, 2017; 136
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1203/831
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1203/119
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1820
2021-01-06T13:13:53Z
3:ART
The effect of combination of active fraction Andrographis paniculata (Burm.f) Ness and Centella asiatica (i) Urban on the alpha glucocidase inhibitor and antioxidant activities
Rusman, Arman; Master program, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
Nugroho, Agung Endro; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281
Pramono, Suwidjiyo; Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281
The Andrographis panicullata and Centella asistica extract have been reported that had a anti-diabetic effect. However, the specific mechanism and the effect combination of both were not yet reported. This study was purposed to determine the potency of extract, fractions and the combination of Andrographis panicullata (AP) and Centella asistica (CA) active fraction to inhibit alpha glucosidase enzymeand its ability to reduce DPPH radical.AP and CA were extracted using 50% ethanol then fractionated with solvents under different polarity levels. The inhibiting activity to alpha glucosidase enzyme and antioxidant activity of each fractions was tested. The most active fractions from AP and CA were then combined and re-tested for activity. The results result reported that both of AP and CA had inhibition of alpha glucosidase activity and antioxidant activity. Based on calculation combination index (CI) of of active fraction of AP and CA showing in alpha glucocidase activity had a antagonist action and antioxidant had a sinergic action. Therefore, combination of AP and CA not has not recomended for alpha glucocidase inhibitor but the combination has ability to reduce DPPH radical.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-04-09
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1820
10.14499/indonesianjpharm31iss1pp56
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 56
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1820/945
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1820/297
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/953
2017-09-11T04:56:22Z
3:ART
DESCRIPTION OF PATIENTS' COMPLIANCE IN ASEER REGION, KINGDOM OF SAUDI ARABIA
Alakhali, Khaled
Medication non-adherence can cause impairments that interfere with self-care activities in individuals who suffer from multiple comorbid diseases that require careful management. The objective of this study is to assess and describe medication adherence in patients of Aseer Region of Kingdom of Saudi Arabia (KSA). Samples of 416 patients in the outpatient department at Abha General Hospital and Aseer Central Hospital are selected randomly. The patients were interviewed using seven a self-administered close-ended anonymous questionnaire. Only descriptive statistics is reported in this study for analyze the compliance category. The participants in the study were 71% (295 out of 416 patients) of participants were females, while 29% (121 out of 416) were males. The participants’ age ranges between 18-60 years. The adherence level was 85%. The major reasons for non-adherence were forgetfulness and feeling good. The study revealed the appropriate level of adherence by participants. The study also revealed that pharmacists and other healthcare professional intervention will help to improve further the medication adherence with possible fruitful therapeutic outcomes of the patients.Key words: Non-adherence patient, Kingdom of Saudi ArabiaMedication non-adherence can cause impairments that interfere with self-care activities in individuals who suffer from multiple comorbid diseases that require careful management. The objective of this study is to assess and describe medication adherence in patients of Aseer Region of Kingdom of Saudi Arabia (KSA). Samples of 416 patients in the outpatient department at Abha General Hospital and Aseer Central Hospital are selected randomly. The patients were interviewed using seven a self-administered close-ended anonymous questionnaire. Only descriptive statistics is reported in this study for analyze the compliance category. The participants in the study were 71% (295 out of 416 patients) of participants were females, while 29% (121 out of 416) were males. The participants’ age ranges between 18-60 years. The adherence level was 85%. The major reasons for non-adherence were forgetfulness and feeling good. The study revealed the appropriate level of adherence by participants. The study also revealed that pharmacists and other healthcare professional intervention will help to improve further the medication adherence with possible fruitful therapeutic outcomes of the patients.Key words: Non-adherence patient, Kingdom of Saudi Arabia
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2015-11-13
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/953
10.14499/indonesianjpharm26iss4pp228
Indonesian Journal of Pharmacy; Vol 26 No 4, 2015; 228
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/953/743
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1221
2019-10-10T01:30:23Z
3:ART
Effect of Atorvastatin Treatment on Vascular Aterogenic Factors (Lipid Profiles and VCAM-1) in Patient Diabetes with Dyslipidemia
Mukti, Asri Wido; Airlangga University
Atorvastatin, Adhesion molecules, Diabetes mellitus, , dyslipidemia, VCAM-1.
To analyze effectiveness of atorvastatin 20mg on lipid profiles and adhesion molecule VCAM-1 in patient with diabetes dyslipidemia. An observational prospective cohort study was conducted from November 2016 to March 2017. Patients who fulfilled the inclusion criteria were taken twice for their lipid profiles and VCAM-1 measurements (before initiation of study and after 6 weeks treatment of atorvastatin 20mg). There were 13 patients who met the inclusion criteria. The results of 13 patients showed that after 6 weeks of atorvastatin therapy, there was a 28% decrease in total cholesterol (t0=223.77±49.69, t1=160.92±24.69), 39% LDL decrease (t0=152.59±44.25, t1 =93±21.44), a decrease in TG 38.6% (t0=200.85±101.53, t1=123.30±62.77) and a statistically significant decrease in VCAM-1 7.47% (t0=729.59±208.06, t1=675.06±182.88). The results of the correlation test between total cholesterol and VCAM-1 (p=0.185, r=0.268), LDL and VCAM-1 (p=0.127, r=0.307), TG and VCAM-1 (p=0.198, r=0.261) showed no correlation. Based on the results of the study, it can be concluded that atorvastatin therapy can provide improvements in atherogenic factors such as decreased lipid profile and VCAM-1, and there was no correlation between lipid profile and VCAM-1 in type 2 DM patients with dyslipidemia.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-04-11
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1221
10.14499/indonesianjpharm30iss2pp128-132
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 128-132
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1221/899
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1495
2019-10-10T01:30:23Z
3:ART
Evaluation of Pain Scale Decrease and Adverse Effects of Ketorolac Injections: An Observational Study in Patients with Postoperative Pain
Ihsan, Mawardi; Department of Pharmacology and Clinical Pharmacy, Universitas Gadjah Mada
Kurniawati, Fivy; Department of Pharmacology and Clinical Pharmacy, Universitas Gadjah Mada
Khoirunnisa, Husna; Undergraduate Program of Pharmacy, Universitas Gadjah Mada
Chairini, Belladonna; Undergraduate Program of Pharmacy, Universitas Gadjah Mada
The use of ketorolac injections in Indonesia is restricted with the provision of 2-3 ampoules per day with a maximum of two days even though the literature states that ketorolac could be used for no more than five days. This study aimed to determine the decrease in pain scale as well as gastrointestinal and renal adverse effects of ketorolac injections in two days of use. This study was an observational study with one-group pre-test post-test design conducted prospectively. The group was a group of patients with postoperative pain who received ketorolac injections and were treated during January till April 2018 in an academic hospital in Yogyakarta. The results showed that ketorolac injections did not provide a statistically significant decrease in pain scale in two days of use compared to before surgery (median [range] = 2.0[0.0-9.33] vs 1.33[0.0-8.33]; p=0.32). Ketorolac injections decreased the kidney function of subjects in two days of use compared to before surgery based on creatinine values (0.76mg/dL vs 0.80mg/dL; p=0.024) and GFR (96.13mL/min/m2 vs 87.52mL/min/m2; p=0.023), and as many as 31 subjects (43.06%) experienced complaints that were suspected to be the gastrointestinal adverse effects of ketorolac injections with the three most complaints were bloating (18.06%), nausea (16.67%), and heartburn (15.28%). Those three results support the use of ketorolac injections following what has been regulated in the Indonesian National Formulary.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-05-31
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1495
10.14499/indonesianjpharm30iss2pp133-140
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 133-140
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1495/903
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/665
2020-01-27T02:51:49Z
3:ART
CURRENT CHALLENGES IN THE DEVELOPMENT AND FORMULATION OF AMORPHOUS SOLIDS
Kumar, Gannu Praveen; Department of Industrial Pharmacy
St. Peter’s Institute of Pharmaceutical Sciences 2-4-1211, Vidyanagar Hanamkonda,
Warangal, India
Kumar, Bakka Sravan; Talla Pamavathi college of Pharmacy, Orus,
Kareemabad, Warangal (AP) India – 506012
Interest in amorphous pharmaceutical systems is steadily growing over the last 10 years. The amorphous state is critical in determining the solid state, physical and chemical properties of many pharmaceutical dosage forms. The main reason of the growing interest toward amorphous materials is the need to improve the bioavailability of drugs with poor aqueous solubility. Many drugs exist in crystalline solid form due to reasons of stability and ease of handling during the various stages of drug development. Conversion of the crystalline to amorphous form may occur during various pharmaceutical processes. This could change the dissolution rate and transport characteristics of the drug. The current focus of research in this area is to understand the origins of amorphous forms. The various thermodynamic phenomena such as glass transition, fragility, molecular mobility, devitrification kinetics, molecular level chemical interactions, solubility and stability are critically discussed. The aim of this review is to understand the recent development in the area of amorphous state and to address the current challenges faced by pharmaceutical formulation and process development scientists and thus is mandatory to anticipate future developments.Key words: Crystalline solids, amorphous forms, characterization, stabilization.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/665
10.14499/indonesianjpharm23iss2pp65-83
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 65-83
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/665/531
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1289
2018-10-23T02:19:20Z
3:ART
In Vitro Evaluation of Anti-Inflammatory and Anti-Diabetic Potential Effects of Euphorbia Tithymaloides Ethanol Extract
Wandita, Theresia Galuh; Departement of Animal Life and Environmental Science, HANKYONG NATIONAL UNIVERSITY, SOUTH KOREA
Joshi, Najuma; Departement of Animal Life and Environmental Science, HANKYONG NATIONAL UNIVERSITY, SOUTH KOREA
dela Cruz, Joseph Flores; Department of Basic Veterinary Sciences
College of Veterinary Medicine
University of the Philippines Los Banos, PHILIPPINES
Hwang, Seong Gu; Departement of Animal Life and Environmental Science, HANKYONG NATIONAL UNIVERSITY, SOUTH KOREA
Hibiscus rosasinensis, glipizide, mucilage, antidiabetic activity, excipients
This research work aims to develop glipizide tablets using Hibiscus rosasinensis leaves mucilage. The mucilage was extracted by using double distilled water and precipitated with ethanol. The precipitated mucilage was dried and grounded into powder. The tablet utilizing the mucilage as excipient was prepared by wet granulation method. The tablets were subjected to various tests. The evaluatory parameters of tablets were found to be within the limits as per United States Pharmacopoeia NF 24/19. FTIR spectrum reveals that there is no incompatibility between the ingredients used. Diabetes was induced in wistar albino rats using streptozotocin and effect of formulation on blood glucose level was determined. It was observed that the formulation could not sustain the release of glipizide. However, the glipizide release was retarded as the amount of mucilage was increased. It was observed that on the completion of antidiabetic study, the formulation could bring the blood glucose level to normal in group rats. However, the blood glucose level was still elevated in group administered with pure gliplizide. It can be concluded that HRM can be used to formulate glipizide tablets. The formulations with HRM shows better hypoglycemic activity and this may be attributed to antidiabetic activity of Hibiscus rosasinensis.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-03-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1289
10.14499/indonesianjpharm29iss1pp1
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 1
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1289/858
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/816
2017-09-11T06:31:39Z
3:ART
EFFECT OF DIALYZER REUSE UPON UREA REDUCTION RATIO (URR), KT/V UREA AND SERUM ALBUMIN IN REGULAR HEMODIALYSIS PATIENT
R. Dewi, Ni Made Amelia; Post Graduate Program of Clinical Pharmacy, Airlangga University, 60268
Suprapti, Budi; Department of Clinical Pharmacy, Airlangga University, 60268
Widiana, I Gde Raka; Nephrology Unit, Internal Medicine Department of RSUP Sanglah Denpasar
Jalan Diponegoro, Denpasar, Bali 80114
Reuse of dialyzers for hemodialysis can help in bringing down the cost of hemodialysis. On the other hand reuse of dialyzer may change dialyzer integrity. This study was undertaken to determine dialyzer reuse effect on Urea Reduction Ratio (URR), Kt/V urea and serum albumin. This was prospective study in Sanglah Public General Hospital Denpasar. Inclusion criteria for this study were patients who receive hemodialysis more than 3 months on twice weekly hemodialysis. In the study we used hollow fiber or dialyzer Elisio type H-130H reprocessed with Renaline automatically by machine renatron. After each session blood urea, post dialysis weight and serum albumin were measured. Measurements was performed on the use new dialyzer, 1st reuse, 4th reuse and 7th reuse. The dialyzer was discarded, if TCV fell below 80% of baseline value. Kt/V and urea reduction ratio (URR) were calculated as measure of dialysis adequacy. A total of 23 people completed the study. There was a lack of uniformity duration of hemodialysis, so that we also performed an analysis using a uniform length hemodialysis duration (4.5 hours) with 15 samples. There were no significant difference between URR and Kt/V urea of new dialyzer and dialyzer reprocessed by renaline respectfully with p=0.131 and p=0.373. If we analyzed only using uniform time of dialysis (4.5 h) the value of URR and Kt/V urea between new and reused dialyzer not significantly different with p=0.520 and p=0.784. There was also not found significant differences between serum albumin of the use new dialyzer and reused dialyzer by the time of uniform or non-uniform, respectfully with p=0.271 and 0.073. Reuse dialyzer does not alter efficacy of hemodialysis.Key words : Dialyzer, Urea Reduction Ratio (URR), Kt/V Urea, Albumin, Total Cell Volume (TCV)
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2015-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/816
10.14499/indonesianjpharm25iss3pp166
Indonesian Journal of Pharmacy; Vol 26 No 3, 2015; 166
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/816/745
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1102
2017-09-11T06:09:12Z
3:ART
EVALUATION OF THE EFFECTIVENESS OF FREE NICOTINE PATCH THERAPY IN A CHARITY CLINIC FOR SMOKING CESSATION
ALI, SYED WAJID; KING SAUD UNIVERSITY
Alghadeer, Sultan Alghadeer; ksu
Alarifi, Mohammed N; ksu
Babelghaith, Salmeen D; ksu
The purpose of this study was to evaluate the efficacy of a free nicotine- patch therapy for smoking cessation in Saudi smokers. A single centered prospective study was carried out in a charity clinic for smoking cessation in Riyadh, Saudi Arabia. A total of 31 subjects who attended the smoking cessation clinic from June 2014 to August 2014 were studied. All participants were male and their mean age was 31.1±6.4 years. The duration of history of smoking was 12.9±6.8 years. The nicotine- patch therapy outcomes were measured at baseline and at 6 weeks after using nicotine- patch therapy. At base line the number of cigarettes per day was 27±10 and carbon monoxide (CO) level was 20.2±8.3. The analyzed statistics revealed that there were significant decreased in the number of cigarette per day (p=0.001) and Carbon Monoxide (CO) level (p=0.001) over 6 weeks of nicotine- patch therapy. After 6 weeks of therapy, abstinence rate was 58 % (verified by CO level) and no serious adverse reactions were documented. The most common side effects were nausea, headache and local irritation sings. In addition, our finding revealed that smokers were likely to suffer from withdrawal symptoms following trying to quit. These withdrawal symptoms include sleep disturbance, loss of concentration and weight gain as well as irritability. Results of this study show that free nicotine-patch therapy is an effective measure for smoking cessation in Saudi population.Keywords: nicotine patch therapy, smoking cessation clinic, side effects
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-12-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1102
10.14499/indonesianjpharm27iss4pp226
Indonesian Journal of Pharmacy; Vol 27 No 4, 2016; 226
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1102/797
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1102/97
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/440
2020-02-19T02:37:07Z
3:ART
PROTOPLAST SUSPENSION CULTURE OF LEAVES MESOPHYLOF Centella asiatica (L.) Urban AND QUALITATIVE ANALYSIS ASIATICOSIDE
Prihastanti, Erma; Faculty of Science and Mathematic, Diponegoro University
Semarang
Soegihardjo, C. J.; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
Purbaningsih, Susiani; Faculty of Biology
Universitas Gadjah Mada
Yogyakarta
Centella asiatica leaves has been used as diuretic, antihypertensi, anti leprae, skin infection, burning skin and celloid. Cells suspenson culture of mesophyll of Centella asiatica leaves were carried out in three steps: isolation, purification, and cell culture. The aim of this research is to investigate the influences of leaves position (age of leaves) and concentration of macerozyme R-10 for cells number and viability, and the influence of sucrose concentration for cells growth and biosynthesis of asiaticoside. Isolation and purification of mesophyll cell has been performed using factorial completely randomized design. The first factor was leaf position (age of leaves), and the second factor was concentration of macerozyme. Collected data were analysed using Anova and Duncan’s test at 0.0 – 2.5 – 5.0 and 7.5 per cent respectively. Asiaticoside produced by cell biomass were analyzed qualitatively using thin layer chro- matography. The result revealed that mesophyll cells could be used as explant for suspension culture. The highest biomass produced with highest viability were found in cells isolated from second leaves treated with 0.1% macerozyme (1.32 . 107 cells/ml). Addition of sucrose to suspension culture medium produced higher packed cells volume (pcv) percentage than no sucrose was added, (0% sucrose gave pcv 8.5%, 2.5% sucrose pcv 22%, sucrose 5% pcv 21.5%, and 7.5% sucrose pcv 15.75%). The asiaticoside production not only depended on sucrose, because without any sucrose was added, the asiaticoside was also available..Key Words: Cell suspension culture, mesophyll, Centella asiatica (L.) Urban, asiaticoside
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/440
10.14499/indonesianjpharm0iss0pp10-19
Indonesian Journal of Pharmacy; Vol 12 No 1, 2001; 10-19
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/440/319
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1184
2018-10-23T02:19:20Z
3:ART
The Effect of Dual Antiplatelet Post Percutaneous Coronary Intervention On Aggregation of Platelet In Myocardial Infarction Patients With Diabetes Mellitus and Non Diabetes Mellitus
Rahmawati, Yessi Asli; Airlangga University
Yogiarto, Mohammad; Dr.Soetomo Teaching Hospital Surabaya
Zulkarnaen, Bambang Subakti; Airlangga University
To analyze the differences in the effect of dual antiplatelet post PCI on the percentage of aggregation in myocardial infarction patients with DM and non DM. Percentage of aggregation were analyzed using light transmission aggregometry (LTA) before loading dose, after PCI, and after maintenance dose of dual antiplatelet (aspirin 100mg and clopidogrel 75mg). Total 22 patients were participated in this study divided into 10 and 12 patients in diabetic and non diabetic group. Percentage of aggregation after taking dual antiplatelet maintenace dose decrease significantly in both group (p=0.006 in diabetic group and p=0.002 in non diabetic group). Mean reduction of percentage of aggregation in diabetic group (3.30±2.91%) is less than non diabetic group (6.83±5.97%). Statistical analysis shows that the mean reduction of percentage of aggregation between two groups were not significantly different (p>0.05). Mean percentage of aggregation after dual antiplatelet maintenance dose was higher in diabetic group and mean reduction of percentage of aggregation was higher in non diabetic group, although statistically in both group it is not significantly different.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-01-17
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1184
10.14499/indonesianjpharm29iss1pp44
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 44
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1184/862
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1740
2021-01-06T13:13:53Z
3:ART
Cytotoxic Potential of Arthrospira platensis Extract on Cervical Cancer Cells Line Hela: Study on Antiproliferative, Cell Cycle, Apoptosis Induction and Anti Metastasis
Nurani, Laela Hayu; Faculty of Pharmacy, Universitas Ahmad Dahlan
Mahfudh, Nurkhasanah; Faculty of Pharmacy, Universitas Ahmad Dahlan
Gandjar, Ibnu Gholib
Rahayu, Intan; Faculty of Pharmacy, Universitas Ahmad Dahlan
A. platensis; cervical cancer; HeLa cells; HFDa cells
Cervical cancer can be treated conventionally with chemotherapy agents, but its use has side effects and complications in the form of damage to normal cells. This study aims to determine the potential of A. platensis as an alternative anticancer agent that is selective towards normal cells. Based on TLC analysis, A. platensis contains antioxidant compounds such as β-carotene, flavonoids, and terpenoids which are able to inhibit proliferation and trigger apoptosis of cancer cells. The study was conducted using cervical cancer cells HeLa and normal cells HDFa. A. platensis macerated with 96% ethanol at a ratio of 1:4. Based on probit analysis, it is known that ethanol extract of A. platensis has a cytotoxic effect on HeLa cells with IC50 values of 260.444μg/mL and index selectivity towards HDFa cells of 7.931. The mechanism of cytotoxic activity of ethanol extract of A. platensis is related to its ability to extend the doubling time, increase the induction of apoptosis, and reduce the rate of cells migration. Ethanol extract of A. platensis can also increase cells accumulation in the S phase to prevent cells from entering the G2/M phase.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-04-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1740
10.14499/indonesianjpharm31iss1pp19
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 19
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1740/939
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1740/281
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1232
2018-01-09T08:39:26Z
3:ART
PHARMACODYNAMICS STUDY OF ETHANOL EXTRACT OF CYCLEA BARBATA (MIERS.) LEAVES ON SRF AND COX-2 GASTRIC MICE WITH NSAID GASTROPATHY
Pribadi, Florence; Universitas Airlangga, Jl Prof. Dr. Moestopo no 47, Surabaya, Indonesia
Suhartati, Suhartati
Basori, Achmad
Aspirin-induced gastropathy, COX-2, SRF, NSAIDs
Epidemiology of NSAIDs gastropathy is increasing as increase number of usage. From arthritis to cardiovascular events and cancer prevention, the versatility of NSAIDs is not questioned. However, no dose of NSAIDs is safe. No matter low dose, or single use, NSAIDs will cause gastric damage upto 3-7 days after use. In inflammation and healing process of gastropathy there are various proteins involved, but treatment with COX-2 and SRF are associated with an immediate healing and better quality of gastric mucosa. Cyclea barbata (miers.) has been declared as functional food for preventing and treatment gastropathy yet, its mechanism of actions have not yet clearly discovered. Hence the aim of this study is to analyze the effects of Cyclea barbata (miers.) ethanol extract to COX-2 and SRF in gastric tissue, in time series basis. Laboratory mice was induced with aspirin to produce gastropathy and then treated with Cyclea barbata (miers.) extract for 1, 3, 7, 10 or 14 days. Gastric tissue then harvested and analysed with elisa procedure to determine tissue SRF and COX-2 level. Treatment was proven to increase COX-2 and SRF higher than control group. This concludes one of Cyclea barbata (miers.) mechanism for NSAIDs gastropathy is by increasing tissue COX-2 and SRF.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-08-08
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1232
10.14499/indonesianjpharm28iss3pp131
Indonesian Journal of Pharmacy; Vol 28 No 3, 2017; 131
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1232/830
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1506
2019-10-10T01:29:33Z
3:ART
Inhibitory Activity of Sargassum hystrix Extract and Its Methanolic Fractions on Inhibiting α-Glucosidase Activity
Azizi, Wirdatul Auliya; Department of Fisheries Faculty of Agriculture Universitas Gadjah Mada Jalan Flora Gedung Perikanan A4 Bulaksumur Yogyakarta 55281
Ekantari, Nurfitri; Department of Fisheries Faculty of Agriculture Universitas Gadjah Mada Jalan Flora Gedung Perikanan A4 Bulaksumur Yogyakarta 55281
Husni, Amir; Department of Fisheries Faculty of Agriculture Universitas Gadjah Mada Jalan Flora Gedung Perikanan A4 Bulaksumur Yogyakarta 55281
α-Glucosidase, Fraction, Methanol, Sargassum hystrix
Seaweed has a great potential in the pharmaceutical field, one of them as antidiabetic. The purposed of this study was to know the inhibitory activity of Sargassum hystrix extract and its methanol fraction in inhibiting α-glucosidase activity. S. hystrix was extracted using methanol, then partitioned using chloroform, ethyl acetate, and methanol. Methanol fraction then separated by column chromatography to obtain the compound. The crude extract, the partitioned methanol fraction, and the column chromatography fraction were tested for its activity on inhibiting the α-glucosidase. The compounds of active fraction were analyzed using gas chromatography-mass spectrometry (GC-MS). The inhibitory activity (IC50) of the crude extracts and the partitioned methanol fraction were 0.35±0.05 and 0.02±0.00 (mg/mL), respectively. The column chromategraphy fractions that had an inhibitory activity to α-glucosidase were M2 (23.46±1.63%), M3 (30.88±4.53%), M4 (73.64±3.47%), and M7 (53.48±1.56%). GC-MS showed that the suspected compound which had inhibiting α-glucosidase in methanol fraction were 9-Octadecenoic acid, 1-Heptadecanecarboxylic acid,9,12-Octadecadienoic acid (Z, Z), and Octadecanoic acid methyl ester.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-03-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1506
10.14499/indonesianjpharm30iss1pp36
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 36
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1506/891
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/521
2018-09-05T01:49:55Z
3:ART
EVALUATION OF ANTIMICROBIAL POTENTIALS OF Cardiospermum halicacabum Linn.
Veerappan, Loganathan; School of Pharmacy, Taylor’s University, 47500 Subang Jaya, Selangor, Malaysia.
T, Thirunala Sundari; Dept. of Biotechnology, Bharathidasan University, Tiruchirapalli, Tamil Nadu, 620 024, India.
N, Pradheepa; Dept. of Biotechnology, Mother Theresa Women University, Kodaikanal, 624101 India.
Antimicrobial activity of Cardiospermum halicacabum shoot extracts were studied on gram-positive bacteria such as Staphylococcus aureus, Bacillus substilis, and gram-negative bacteria such as E.coli, Proteus vulgaris, and fungus Candida albicans. Disc diffusion method was used to study the antimicrobial activity of aqueous, ethanol, chloroform and ether extracts of C. halicacabum. Ampicillin was used as reference standard at 10 mg/disc concentration. Extracts of C. halicacabum exhibited a significant antibacterial activity except the aqueous extract. Ethanolic extract was found to be very effective with maximum activity index (0.84). The ethanolic extract exhibited minimum inhibitory concentration (MIC of 0.25 mg/mL against Staphylococcus aureus, Bacillus substilis, E. coli and Proteus vulgaris and 0.125 mg/mL against Candida albicans. The MIC of chloroform and ether extracts ranged between 0.25 and 1.0 mg/ml against the test organisms. All the extracts showed antifungal activity against Candida albicans. Key words: antimicrobial activity, Cardiospermum halicacabum shoot system, solvent extraction,.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2013-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/521
10.14499/indonesianjpharm24iss1pp61-64
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 61-64
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/521/399
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1237
2020-03-18T10:32:19Z
3:ART
Analysis of Factors Influencing the Availability of Medicine During JKN Era
Satibi, Satibi; Faculty of Pharmacy, Universitas Gadjah Mada
Ranowijaya, Ranowijaya; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
Aswandi, Aswandi; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
Bermalam, Junagsti; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
Widodo, Gunawan Pamudji; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
JKN; availability of drugs factor; doctors factor; pharmacists factor and patients factor
The availability of medicine is important during National Social Healthy Insurance (JKN) that put into effect in the hospital, one of health service facility. The purpose of this study was to look of the availability of medicines was during JKN era and what factors influence the availability in three Region General Hospitals in Sulawesi. This study was conducted using descriptive method of analytic to get a correlation among doctors, pharmacists and patients as independent variables toward the availability of medicine as dependent variable. The study has been performed in 3 hospitals in Sulawesi. This data obtained were analyzed using statistical model of Linier Regression Analysis with F test or ANOVA test and t test. R was the value of regression which showed the influence of one to another variable. The results showed the level of medicine availability in hospital A was 12.49 months. Factors that influence the availability of medicines were doctors (R=0.778, p= 0.000), pharmacists (R=0.619, p= 0.000) and patients (R=0.653, p= 0.000). All there factor significantly affect the availability of medicines in Hospital A (R=0.675, p=0.000). Hospital B had an excess supply of medicines, with the average of 23.31 month. Factors that influenced the availability of medicines in Hospital B was doctor (R=0.877), pharmacist (R=0.861) and patient (R=0.984) (p=0.000 all). From multiple regression there factors showed a significant correlation to the availability of medicines (R=0.901, p=0.000). Similarity on Hospital A and B, data of the availability of medicine in hospital C was categorized excess as the average of stock was 56.01 month. Factors that influenced the availability of medicines in Hospital C was doctor (R=0.753, p= 0.000), pharmacist (R=0.869, p= 0.000) and patient (R=0.545, p=0.001).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-03-12
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1237
10.14499/indonesianjpharm29iss1pp37
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 37
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1237/837
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/512
2018-09-05T01:49:54Z
3:ART
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/658
2020-01-27T02:53:58Z
3:ART
HEPATOPROTECTIVE EFFECT OF GAMAVUTON-0 AGAINST D˗GALACTOSAMINE/LIPOPOLYSACCHARIDE-INDUCED FULMINANT HEPATIC FAILURE
Nurrochmad, Arief; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta 55281,
Indonesia
Sari, Ika Puspita; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta 55281,
Indonesia
Murwanti, Retno; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta 55281,
Indonesia
., Sardjiman; Curcumin Research Center, Faculty of Pharmacy, Universitas Gadjah Mada
Yogyakarta 55281, Indonesia.
Candraningrum, Triana; Curcumin Research Center, Faculty of Pharmacy, Universitas Gadjah Mada
Yogyakarta 55281, Indonesia.
Afritasari, Dyah; Curcumin Research Center, Faculty of Pharmacy, Universitas Gadjah Mada
Yogyakarta 55281, Indonesia.
Martina, Devina; Curcumin Research Center, Faculty of Pharmacy, Universitas Gadjah Mada
Yogyakarta 55281, Indonesia.
Siahaan, Iren Wati; Curcumin Research Center, Faculty of Pharmacy, Universitas Gadjah Mada
Yogyakarta 55281, Indonesia.
The objective of this study is to determine the hepatoprotective effect of GVT-0 (one of curcumin analogues) against liver damage in rat-induced D-galactosamine (D-GalN)/lipopolysaccharide (LPS) as a model of fulminant hepatitis. In the study D˗GalN/LPS elevated serum GPT activity that indicate a particular occurrence of liver damage due to depletion of UTP and UDP-glucuronic acid. Administration of GVT-0 (10 mg/kg) showed decreased enzyme activity of SGPT/SGOT but had no effect on serum ALP and total bilirubin levels, whereas at doses of 20 and 40 mg/kg, the protective effect of GVT-0 was decrease. The glutathione content in the D-GalN/LPS (0.76 ± 0.07) mol/g liver content was found lower than controls (0.90 ± 0.03) mol/g liver. Administration of GVT-0 dose of 10, 20 and 40 mg/kg restored glutathione content returned to normal levels. The results showed that treatment of GVT-0 showed no effect on TBARS and catalase activity. Treatment of D-GalN/LPS, indicating the trend of increased TNF-α, although statistically not significant, while the administration of GVT-0 showed a tendency to decrease the concentration of TNF-α. All findings of the results indicated that the GVT-0 mainly lower dose (10 mg/kg) showed hepatoprotective action in rat model of fulminant hepatitis induced by D-GalN/LPS. The results indicated that the mechanism of hepatoprotective effect of GVT-0 is not via antioxidant properties of GVT-0. However, further studies are necessary to explain the molecular mechanism of hepatoprotective effect of GVT-0.Key words: Gamavuton-0, hepatoprotective, fulminan hepatitis, D˗galactosamine/LPS
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/658
10.14499/indonesianjpharm23iss1pp18-26
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 18-26
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/658/524
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1064
2017-09-11T06:10:22Z
3:ART
SYNTHESIS AND ANALGETIC ACTIVITY EVALUATION OF 4-[N-(4-HYDROXYPHENYL)CARBOXYMIDOYL]-2-METHOXYPHENOL
Pudjono, Pudjono; Department of Pharmaceutical Chemistry. Faculty of Pharmacy, Universitas Gadjah Mada
Yogyakarta 55281
Anindita, Jessica
Hakim, Arief Rahman
Purnomo, Hari
Paracetamol is an analgesic-antipyretic compound derived from p-aminophenol. Though paracetamol has good efficacy and safety on consumption, parasetamol has hepatotoxic effect as its adverse drug reaction. 4-[N-(4-hydroxyphenyl)carboxymidoyl]-2-methoxyphenol is one of p-aminophenol derivative that was already been determined in silico using molecular docking PLANTS method, and it was known that 4-[N-(4-hydroxyphenyl) carboxymidoyl]-2-methoxyphenol has analgesic effects more potent and has hepatotoxic adverse effect lower than paracetamol. 4-[N-(4-hydroxyphenyl)carboxymidoyl]-2-methoxy-phenol can be synthesized through reaction of p-aminophenol with vanillin under acid condition. The synthesized products were recrytalized, dried, and the purity was determined with melting point determination and Thin Layer Chromatography. The structure of pure crystals were elucidated using IR, 1H-NMR, C-NMR, and Mass Spectroscopy. The analgesic evaluation was carried in vivo using writhing test method. The synthesized compound were divided into three dosage variations, 0,5; 1; and 2 mol equivalent to 100 mg/kgBB of paracetamol (reference drug). 4-[N-(4-hydroxyphenyl)carboxymidoyl]-2-methoxyphenol with 1 mol dosage has analgesic activity better than paracetamol but the difference was not significant.Keywords: 4-[N-(4-hydroxyphenyl)carboxymidoyl]-2-methoxyphenol, p-aminophenol, analgesic, writhing test
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-03-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1064
10.14499/indonesianjpharm27iss2pp99
Indonesian Journal of Pharmacy; Vol 27 No 2, 2016; 99
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1064/775
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1487
2019-10-28T01:20:02Z
3:ART
Phytochemical analysis, antioxidant, antibacterial and antidiarrheal activities of Limnophila repens
Akter, Afroza; Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
Basher, Mohammad Anwarul; Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
Roy, Roni; Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
Sultana, Sharmin; Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
Daula, A F M Shahid Ud; Department of Pharmacy, Noakhali Science and Technology University, Sonapur, Noakhali-3814, Bangladesh.
Limnophila repens; phytochemical content; antioxidant; antibacterial; antidiarrheal.
The study was carried out to determine the phytochemical content, antioxidant, antibacterial and antidiarrheal activity of methanolic extract of whole plant of Limnophila repens. The total phenolic, flavonoids, flavonols, tannins, β-carotene, lycopene, chlorophyll-α, and chlorophyll-β contents were found to be 65.21 ± 0.004 mg GAE/g , 44.83 ± 0.003 mg QE/g , 17.21 ± 0.002 mg QE/g, 56.27 ± 0.002 mg GAE/g , 1.32 ± 0.01 µg/g, 0.93 ± 0.012 µg/g, 11.85 ± 0.04 mg/g and 9.69 ± 0.037 mg/g, respectively. In vitro antioxidant potential of L. repens was assessed using various methods like by 2,2-diphenyl-1 picrylhydrazyl (DPPH), hydroxyl radical scavenging assay, reducing power assay, FRAP assay and thiobarbituric acid assay. IC50 values of the aforesaid tests were found to be 2.33 mg/ml, 442.31 µg/ml, 374 µg/ml, 55.09 µg/ml, and 4.30 mg/ml, respectively. The total antioxidant capacity was observed 140.7 ± 0.004 mg/g AAE. Aantibacterial activity of the methanolic extracts of whole plant of L. repens was evaluated using the disk diffusion method. The plant extract didn’t show any activity against Gram-positive (except Staphylococcus aureus) and Gram-negative bacteria. Finally, castor oil induced method was conducted to investigate the antidiarrheal activity of L. repens. At the dose of 400 mg/kg, the plant extract (35.62% inhibition of diarrhea) exhibited stronger effect compared to standard drug, loperamide (27.4% inhibition of diarrhea).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-09-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1487
10.14499/indonesianjpharm30iss3pp187
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 187
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1487/917
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/677
2020-01-27T02:53:16Z
3:ART
ELECTROCHEMICAL ANALYSIS OF ZINECARD IN PHARMACEUTICAL AND BIOLOGICAL SAMPLES
Reddy, Chennupalle Nageswara; Electroanalytical lab, Department of Chemistry,
Tirupati – 517 502, A.P., India
Prasad, Puthalapattu Reddy; Department of Chemistry, Govt. Degree College, Koduru, A.P.India
Sreedhar, Neelam Yugandhar; Electroanalytical lab, Department of Chemistry,
Tirupati – 517 502, A.P., India
Zinecard is used to prevent a toxic effect to heart caused by certain medicines that are used to treat cancer. Zinecard is also used to treat tissue damage caused by the leakage of certain medicines that are used to treat cancer. Differential pulse polarographic method developed for the quantitative determination of zinecard gives a peak at -0.32 V at DME. From the structural point of view zinecard contains a >C=Omoiety which can be electrochemically reduced at universal buffer (pH 4.0). Millicoulometric experiment is performed successfully in estimating the number of electrons and proton to understand reduction mechanism. The differential pulse polarographic peak was adequately well-resolved, reproducible and linear dependent with the zinecard concentration. For quantification the calibration plot for zinecard concentrations ranging between 1.0×10-5mol dm-3 to 1.0×10-8mol dm-3 at pH 4.0 was selected. The proposed differential pulse polarographic method was successfully applied to the determination of zinecard in pharmaceutical formulations and urine samples.Key words: Zinecard, DPP, pharmaceutical formulations and urine samples.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/677
10.14499/indonesianjpharm23iss3pp147-155
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 147-155
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/677/543
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1357
2018-11-02T06:46:53Z
3:ART
Pharmaceutical applications of Aloe vera
Heng, Huay Chin; Faculty of Pharmacy, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia
Zulfakar, Mohd Hanif; Centre for Drug Delivery Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia
Ng, Pei Yuen; Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia,
50300 Kuala Lumpur, Malaysia
A. vera; anti-diabetic; anti-inflammatory; wound healing; antibacterial
Aloe vera has been used as folk medicine for a host of therapeutic indications of which the inner gel is the component extensively used and studied. Proponents of the use of this plant suggest that it is easily available, economical, and have fewer side effects compared to commercial drug compounds. However, the active constituents and their exact mechanisms have yet to be fully elucidated. This review focuses on the identification of the active constituents and their functional mechanism in the areas of anti-diabetic, anti-inflammatory, wound healing, and antibacterial. Preliminary evidence was found to support the antidiabetic effect which extended to the early stages of the disease with no adverse effects at the dosages used. Although topical application for its anti-inflammatory effect may be delayed and minute, oral administration has shown a significant response. However, too high a dose has been linked with an initial inflammatory reaction. Favorable response to the gel has also been demonstrated to support the use of A. vera in wound-healing and as an antibacterial agent, although limited to simple, uncomplicated wounds. Different active constituents such as acemannan and aloin have been suggested for the effects of the plant and this may be interpreted as synergism among different compounds rather than the action of a single compound. Until robust evidence is available, the plant should only be used as an adjunct to other well-established evidence-based treatment modalities.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Ministry of Education (FRGS/1/2016/SKK09/UKM/02/4)
2018-09-24
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1357
10.14499/indonesianjpharm29iss3pp101
Indonesian Journal of Pharmacy; Vol 29 No 3, 2018; 101
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1357/872
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/656
2020-01-27T02:53:58Z
3:ART
APPLICATION OF FOURIER TRANSFORM INFRARED SPECTROSCOPY FOR QUALITY CONTROL OF PHARMACEUTICAL PRODUCTS: A REVIEW
Rahman, Abdul; Laboratory of Analytical Chemistry, Faculty of
Pharmacy, Universitas Gadjah Mada, 55281,
Yogyakarta, Indonesia. +62274-543120
Analysis of pharmaceutical products covers all aspects of quality control of active pharmaceutical ingredients (API) and finished products. Today, Fourier transform infrared (FTIR)spectroscopy, especially in combination with chemometrics software, has emerged as one of the romising analytical techniques to be used in pharmaceutical industry, for quality control of desired pharmaceutical products. Compared with other instrumental techniques, FTIR spectroscopy offers some advantages, namely it is rapid, simple in sample preparation, and not destructive. In this review, the application of FTIR spectroscopy for qualitative and quantitative determinations of API and monitoring drug release are described.Key words: FTIR spectroscopy, quality control, pharmaceutical products.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/656
10.14499/indonesianjpharm23iss1pp1-8
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 1-8
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/656/522
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1157
2017-09-11T06:07:57Z
3:ART
PREPARATION AND CHARACTERIZATION OF CO-CRYSTALS OF DIACEREIN
Thenge, Raju Rameshrao; Department of Industrial Pharmacy, IBSS College of Pharmacy
Patond, V B; Department of Industrial Pharmacy, IBSS College of Pharmacy
Ajmire, P V; Department of Industrial Pharmacy, IBSS College of Pharmacy
Barde, L N; Department of Industrial Pharmacy, IBSS College of Pharmacy
Mahajan, N M; Department of Pharmaceutics, Dadasaheb Balpande College of Pharmacy
Tekade, N P; SGSPS Institute of Pharmacy
Co-crystals; Diacerein; physicochemical properties; FT-IR
Diacerein, anti-inflammatory drug used in the treatment of osteoarthritis. Being a BCS class II drug, it has poor solubility, dissolution rate and other physicochemical properties. Thus the aim of present study was to prepare co-crystals of diacerein to improve solubility, dissolution rate. The diacerein co-crystals were prepared using urea and tartaric acid as conformer by Solvent drop grinding method. The diacerein co-crystals were characterized by scanning electron microscopy (SEM), FT-IR spectroscopy (FT-IR), Differential scanning calorimetry (DSC) and X-ray diffractometry (XRD). The co-crystals were evaluated for solubility, dissolution rate and other physicochemical properties and compared with commercial diacern sample. The co-crystals exhibit the difference in the size and shape of crystals. The FT-IR spectra of diacerein co-crystals showed slightly different in the characteristic peaks compared to commercial diacerein sample. DSC data indicate the decrease in the melting endotherm of co-crystals compare to diacerein. The co-crystals with urea showed increase and intense peak and co-crystals with tartaric acid showed decreased number of peaks compared to commercial diacerein. The co-crystals of diacerein formulated in to the Tablet and evaluated for tablet properties. The tablet formulation showed improved tablet characteristics as well as dissolution rate compared to commercial diacerein.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-03-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1157
10.14499/indonesianjpharm28iss1pp34
Indonesian Journal of Pharmacy; Vol 28 No 1, 2017; 34
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1157/802
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1646
2021-01-06T13:13:53Z
3:ART
Antidiabetic Activity of Okra Fruit (Abelmoschus esculentus (L) Moench) Extract and Fractions in Two Conditions of Diabetic Rats
Herowati, Rina; Faculty of Pharmacy
Setia Budi University
Indonesia
Abelmoschus esculentus (L) Moench, STZ-NA induced diabetes, insulin resistance diabetes, pancreatic cell regeneration, glucose transporter-4.
Okra (Abelmoschus esculentus (L) Moench) fruit is empirically used in type 2 diabetes mellitus treatment. This research aims to know the antihyperglycemic activity of okra fruit extract and fractions in streptozotocin-nicotinamide (STZ-NA) induced as well as in insulin resistance diabetic rats, the effect on pancreatic cells regeneration, and the effect on immunohistochemical expression of glucose transporter-4. This study used a group of 35 male Wistar rats for STZ-NA induced diabetic model and another group of 35 rats for insulin resistance diabetic model. Gliclazide (0.72mg/kg BW) and metformin (45mg/kg BW) were used as drug control in STZ-NA induced and insulin resistance diabetes, respectively. Okra fruit ethanol extract, n-hexane, ethyl acetate, and water fraction were orally administered with dose of 200; 107; 6 and 86mg/kg BW, respectively, for 28 days after diabetic condition was obtained. Blood glucose level was measured every week. Hematoxylin-eosin staining was used to evaluate the pancreatic cells regeneration, while immunohistochemistry was used to evaluate the expression of glucose transporter-4 in muscle membrane cells, at the end of the treatment. The results revealed that ethyl acetate fraction was the most effective in lowering blood glucose level in both condition of diabetes. Ethyl acetate fraction decreased the necrosis of pancreatic cells in STZ-NA induced diabetic rats and increased the expression of glucose transporter-4 in muscle cell of insulin resistance diabetic rats.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-04-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1646
10.14499/indonesianjpharm31iss1pp27
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 27
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1646/940
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/688
2020-01-27T02:53:31Z
3:ART
PREPARATION AND CHARACTERIZATION OF STAVUDINE ENTRAPPED LIPOSPHERES
Shegokar, Ranjita; C.U. Shah College of Pharmacy, S.N.D.T.
Women’s University, Santacruz (W), Mumbai-400049 India
Singh, Kamalinder; C.U. Shah College of Pharmacy, S.N.D.T.
Women’s University, Santacruz (W), Mumbai-400049 India
Lipospheres of stavudine were prepared by melt dispersion technique using trimyristin, tripalmitin and triastearin, stearic acid, Compritol® 888 ATO and Precirol® ATO 5 as lipid matrix in the various drug-lipid ratios. Drug entrapped free flowing solid lipospheres of triglycerides and glyceryl behenate were characterized for surface morphology, particle size distribution, encapsulation efficiency, and in vitrorelease behavior. The effect of drug lipid ratio, the surfactant used, concentration of stabilizer, and stirring speed were identified as the key variables affecting the formation of discrete spherical lipospheres and sustained drug release rate. The lipospheres production conditions were optimized by using 2% w/w sodium cholate and 1% Plural oleic as a stabilizer. The concentration of lipid used had pronounced effect on particle size of the lipospheres. The incorporation efficiency was found to be in range of 30 to 50%. Increase in concentration of surfactant and stirring speed produced fine spherical, smooth, and round lipospheres. All the prepared lipospheres exhibited slow release profiles dictating the Higuchi mode of release.Key words: Lipospheres, stavudine, lipid matrix, hydrophilic surfactant, drug release
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/688
10.14499/indonesianjpharm23iss4pp231-237
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 231-237
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/688/554
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1446
2019-10-10T01:29:33Z
3:ART
The Force Degradation Study of The Morpholinium 2-((4-(2-Methoxyphenyl)-5-(Pyridin-4-YL)-4H-1,2,4-Triazol-3-YL) Thio) Acetate
Varynskyi, Borys; Zaporozhye State Medical University
Kaplaushenko, Andriy; Zaporozhye State Medical University
1,2,4-triazoles, LC MS, forced degradation
The aim of the current research was to make force degradation study of the morpholinium 2-((4-(2-methoxyphenyl)-5-(pyridin-4-yl)-4H-1,2,4-triazol-3-yl)thio)acetate (API) for additional confirmation of the selectivity of API and impurity determination method, propose possible structures of degradation products. Agilent 1260 Infinity (degasser, binary pump, autosampler, column thermostat, diode array detector) was used. Mass selective detector was single-quadrupole LC/MS 6120 with electrospray ion source (ESI). The chromatography study was carried out by isocratic elution with a water-acetonitrile mixture (84:16) with the addition of 0.1% methanoic acid. A column Zorbax SB-C18, 30 mm x 4.6 mm, 1.8 um. A column temperature was 40oC. The flow rate was 0.400 ml/min. The period of exposition of stress factors was four days. The period of exposition of stress factors was four days. Influence of the such factors as acid, alkali, H2O2, temperature, UV radiation on the API in bulk drug, 0.1% solution and 1% “solution for injection” were studied. Dependence of content of API from a number of days of factors exposition was studied. Most destructive action was during the influence of the H2O2 and UV radiation. API was stable during the storage at laboratory conditions, acid, alkali and temperature (60oC) influence. Some degradation products structure was proposed.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Zaporizhzhia State Medical University
2019-03-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1446
10.14499/indonesianjpharm30iss1pp25
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 25
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1446/890
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1355
2018-11-02T06:46:53Z
3:ART
The Effect of Ethanol Extract of Piper nigrum L. Fruit on Reproductive System in Adult Male Wistar Rats: A Study of FSH, LH, Testosterone Level and Spermatogenic Cells
Ekaputri, Tia Wida; Master Programme, Basic Medical and Biomedical Science, Faculty of Medicine, Universitas Gadjah Mada, Indonesia
Sari, Ika Puspita; Department of Pharmacology & Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada
Rizal, Dicky Moch.; Department of Physiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Indonesia
black pepper (Piper nigrum L.), FSH, LH, male rat, testosterone, spermatogenic cells
Exploration to find out new natural contraceptive agent for male is still being developed. Black pepper (Piper nigrum L.) and its main alkaloid piperine have potential antifertility because of sitotoxic and hormonal effects. The aim of this study is to find out the effect of ethanolic extract of black pepper (Piper nigrum L.) fruit of on reproductive hormone serum level, sperm quality, and spermatogenic cell populations in adult male Wistar rat. Twenty five male rats were divided into five groups consisting of two control group, i.e. K(-) (Na-CMC 0.5%), K(+) (finasteride 0.45mg/kg BW), and three groups received different doses of black pepper fruit ethanolic extract, i.e. D(1) (3.33mg/kg BW), D(2) (6.66mg/kg BW) and D(3) (13.32mg/kg BW) respectively. The treatment were given to each group for 55 days. Reproductive parameters were measured, including serum level of reproductive hormones (FSH, LH, dan testosteron), quality of cauda epididymal sperm (spermatozoa concentration, motility and morphological abnormality), spermatogenic cell populations (primary spermato-cyte and spermatid count) and seminiferous tubules diameter. Ethanol extract of black pepper fruit at doses of 3.33mg/kg BW, 6.66mg/kg BW, and 13.32mg/kg BW increased serum FSH level. Extract at dose of 13.32mg/kg BW decreased serum LH level, while extract at doses of 6.66mg/kg BW and 13.32mg/kg BW decreased serum testosterone level. The number of primary spermatocytes, spermatozoa concentration, and spermatozoa motility were decreased by administration of ethanol extract of black pepper fruit with dose of 6.66mg/kg BW and 13.32mg/kg BW. Ethanol extract of black pepper fruit at dose of 6.66mg/kg BW and 13.32mg/kg BW had a negative impact on the male reproductive system and showing potential antifertility in male rat.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-09-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1355
10.14499/indonesianjpharm29iss3pp136
Indonesian Journal of Pharmacy; Vol 29 No 3, 2018; 136
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1355/875
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1087
2017-11-07T00:57:21Z
3:ART
MONOSODIUM GLUTAMATE EXPOSURE AT EARLY DEVELOPMENTAL STAGE INCREASES APOPTOSIS AND STEREOTYPIC BEHAVIOR RISKS ON ZEBRAFISH (DANIO RERIO) LARVAE
Kurnianingsih, Nia; Departement of Physiology, Faculty of Medicine, Brawijaya University
Utami, Juliyatin Putri; Biomedical Science, Faculty of Medicine, Universitas Brawijaya
Nurdiana, Nurdiana; Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya
Lyrawati, Diana; Department of Pharmacy, Faculty of Medicine, Universitas Brawijaya
Monosodium Glutamate; Early Embryo; Duration; Apoptosis; Behavior
Excessive glutamate may give neurotoxic effects and contribute to Autism spectrum disorder(ASD). In this study, we investigated prolonged exposure effects of 10 µg/mL Monosodium Glutamate (MSG) on intracellular calcium level, bax, bcl-2, ratio of bax/bcl-2 genes expression, caspase-3, apoptosis of brain cells and stereotypic behavior of Zebrafish (Danio rerio) larvae at early developmental stages. Genes expression were determined by real time PCR, caspase-3 using ELISA, intracellular Ca2+ and apoptotic cells of brain using confocal microscopy, locomotor activity by using crossing lines assay whereas stereotypic behavior by circle swimming. The results indicated that MSG exposure increased brain bax and bcl-2; and caspase-3; intracellular Ca2+; and apoptosis; stereotypic behavior; and decreased locomotor activity. Termination of MSG treatments resulted in recovery of bax, bcl-2, caspase-3 basal levels and stereotypic behavior. In conclusion, MSG exposure at early embryonic stage increased brain cell damage and risk of behavior changes.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1087
10.14499/indonesianjpharm27iss3pp128
Indonesian Journal of Pharmacy; Vol 27 No 3, 2016; 128
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1087/787
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1986
2021-01-07T01:00:39Z
3:ART
Design and Synthesis of Novel Derivatives of 4-(6-(4-Substituted Phenyl)-7H-[1,2,4] Triazolo[3,4-b][1,3,4]Thiadiazin-3- yl) Phenol as a Potent Inhibitor of Tubulin with Antitumor Activity
Kubba, Ammar A. Razzak Mahmood; Department of Pharmaceutical Chemistry, College of Pharmacy-University of Baghdad, Bab-Al-Mouadam-10001, Baghdad
Shihab, Wurood Ahmed; Department of Pharmaceutical Chemistry-College of Pharmacy/University of Ashur, Al-waziriya, Baghdad/Iraq
Al-Shawi, Nada Naji; Department of Pharmacology and Toxicology, College of Pharmacy-University of Baghdad, Bab-Al-Mouadam-10001, Baghdad/Iraq
Jabir, Majid Shamki; Division of Biotechnology/ Department of Applied Science/University of Technology, Baghdad/Iraq
Synthesis; novel series; anti-proliferative; tubulin-targeting.
A new series of 4-(6-(4-substituted phenyl)-7H[1,2,4] triazolo[3,4b][1,3,4]thiadiazin-3-yl) phenol was synthesized and explored for anti-tubulin activity using in silico and in vitro assay models. The starting nucleus, ethyl-4-hydroxybenzoate was treated with hydrazine hydrated, and converted to 4-hydroxybenzohydrazide (1). Then, (1) was stirred with CS2 and KOH in dry EtOH to afford 2-(4-. hydroxyl benzoyl)hydrazine-1-carbodithioate, as a potassium salt (2). Compound (2) was used directly and refluxed with hydrazine hydrate to yield a parent nucleus, 4-(4-amino-5-mercapto-4H-1,2,4-triazol-3-yl)phenol (3). The target compounds (4-7), were synthesized by refluxing compound (3) with various substituted phenacyl bromides using sodium acetate as a abase, The new compounds (3-7) were characterized using different spectroscopic analysis techniques including IR, and 1HNMR. The anticipated modes of binding to α- tubulin were also investigated by means of a molecular docking experiment. The binding mode revealed good agreement with the in vitro work with binding energies of (-38.77 and -37.50 kcal/ mol) for the most potent compounds 7 and 4, respectively. The effect of synthetic novel compounds on cancer cell lines proliferation was screened by MTT assay. The ability of the synthetic compounds to induce apoptosis process was tested using acridine orange/ ethidium bromide staining. The synthetic novel compounds exhibited potent inhibitory effects on the growth and viability of cancer cell lines SKOV-3 and AMJ-13 cells. They inhibited the proliferation and growth of cancer cell lines at low concentrations, with IC50 values ranging from10.44 to 19.67 µg/mL against SKOV-3 cells, and for AMJ-13 cells, the IC50 values were11.35 to 20.52 µg/mL. The effect of the synthetic compounds on the cell growth, and proliferation of cancer cell lines was associated with increased apoptosis.Our results demonstrated that the target compounds inhibited cancer cell lines proliferation, with a mechanism of action parallel to that of other tubulin inhibitors
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
University of Baghdad-College of Pharmacy
2020-08-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1986
10.14499/indonesianjpharm31iss2pp92
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 92
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1986/955
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1023
2017-09-11T04:59:09Z
3:ART
STRUCTURE–ANTIMICROBIAL PROPERTIES STUDY OF SOME DIBASIC PHENYLCARBAMIC ACID ESTERS
Malík, Ivan; Dept. of Pharmaceutical Chemistry, Faculty of Pharmacy, Comenius University in Bratislava, Slovak Republic
Csöllei, Jozef; Dept of Chemical Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences in Brno, Czech Republic
Bukovský, Marián; Dept. of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, Slovak Republic
Due to worldwide phenomenon of microbial resistance to commonly used therapeutic agents, antibiotics and antifungals, dibasic di‑/trimethylphenylcarbamic acid esters 1–3, a non-traditional series of potential antimicrobials, has been in vitro evaluated against chosen Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacterial strains as well as against yeast (C. albicans) by the minimum inhibitory concentration (MIC) assay. Taking into consideration chemical structure of tested derivatives, the incorporation of more than one protonated atom of nitrogen into salt forming fragment positively influenced the activity against E. coli. On the contrary, the presence of one or more methyl groups instead of 3-alkoxy side chain attached to lipophilic aromatic moiety has not found to be favorable structural feature. In entire set of inspected compounds, the most promising results have been found for the compound 3, chemically1-[3-piperidinium-1-yl-2-({[(2,4,6-trimethylphenyl)amino]carbonyl}oxy)propyl]piperidinium dichloride, against E. coli with the MIC=1.56 mg/mL. Key words: Dibasic phenylcarbamic acid esters, Escherichia coli, hydrogen bonding, lipophilicity
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1023
10.14499/indonesianjpharm27iss1pp53
Indonesian Journal of Pharmacy; Vol 27 No 1, 2016; 53
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1023/768
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
e671a3204c86b27c18942e47fd5842c7