2024-03-29T13:56:17Z
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/oai
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1810
2021-01-07T01:00:39Z
3:ART
QuEChERS and C18 as solid phase extraction sorbent - ultra-high performance liquid chromatography -ultraviolet-visible method for determination of parabens in cosmetics products
Nguyen, Ngoc-Van Thi; Can Tho University of Medicine and Pharmacy
paraben, cosmetic, QuEChERS, solid phase extraction
Concerns are growing about human exposure to endocrine-disrupting chemicals (EDCs), especially during the preadolescent development stage. Parabens are prevalent EDCs widely used as additives in cosmetics. So, the determination of parabens in such products is important. In this study, we developed a reliable and sensitive method to determine simultaneously nine common parabens (methylparaben, ethylparaben, phenylparaben, benzylparaben, penthylparaben, and two groups of isomeric compounds include propylparaben, isopropyl paraben, and butylparaben, isobutylparaben) in cosmetics products. The QuEChERS and solid-phase extraction techniques are used for extraction parabens from non-surfactant cosmetics (perfume, mouth wash solution) and surfactant cosmetics (shampoo, cream, gel), respectively and quantified by using ultra-performance liquid chromatography coupled with the ultraviolet-visible detector. All nine compounds showed good linearity with regression coefficients predominantly above 0.990. The LOD and LOQ of parabens were 0.07 µg/mL; 0.2 µg/mL, respectively. The recoveries ranged from 80 to 110% with the relative standard deviations below 8%. The developed method was successfully applied to determine parabens in various commercial cosmetic products from a local supermarket and the total parabens concentrations are in a wide-ranged from 2.0 to 1270 mg/kg.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-08-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1810
10.14499/indonesianjpharm31iss2pp69
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 69
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1810/950
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/671
2020-01-27T02:51:49Z
3:ART
INFLUENCE OF Morinda citrifolia, L. FRUIT EXTRACT AS ADJUVANT ON IG-Y PRODUCTION RAISED IN LAYING CHICKENS AGAINST AVIAN INFLUENZA VACCINE
Sasmito, Ediati; Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. Skip Utara Yogyakarta, 55281
Harimurti, Sri; Faculty of Animal Sciences, Universitas Gadjah Mada, Yogyakarta, Indonesia
Nurhidayat, Hendy; Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. Skip Utara Yogyakarta, 55281
Anggoro, Wahyu; Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. Skip Utara Yogyakarta, 55281
Hikmawati, Elika Nur; Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. Skip Utara Yogyakarta, 55281
Wibowo, Joko Tri; Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. Skip Utara Yogyakarta, 55281
Avi, Atut Sulma; Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. Skip Utara Yogyakarta, 55281
Hilmi, Nurul; Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia. Skip Utara Yogyakarta, 55281
Immunized avian with vaccine, produces specific antibody in the serum as well as in the egg yolk. An adjuvant is used to augment the effects of a vaccine by stimulating the immune response. Morinda citrifolia, L., especially the fruit contains of some chemical compounds such as polysaccharide, scopoletin, damnacanthal, proxeronine and proxeroninase. All those of active compounds, are soluble in different solvents and have different activities. Some of those compounds have adjuvant activity. The aim of our study was to observe the capability of M.citrifolia fruit extracts as adjuvant to enhance the specific antibody (IgY) production in immunized laying chickens Lohmann strain (28 weeks old) with a commercially available avian influenza (H5N2) vaccine. To the groups (@6) of laying chickens were orally administered once a day, through out of the study, with n-hexane (group I; divided ino 3 sub groups), ethanolic (group II; divided into 3 subgroups) and aqueous extract (group III; divided into 3 subgroups) of M.citrifolia (prepared in capsules) on dose of 29 mg; 58 mg; and 116 mg/chicken/day, respectively, and group IV as plasebo control and group V as normal control. All of the groups were induced with avian influenza vaccine, except the normal control. Eggs of 3, 6, and 10 weeks after immunized, were collected. Isolation of IgY was performed by repeated polyethylene glycol 6000 precipitation steps. IgY obtained was analyzed by indirect ELISA method. The serum was tested for antibody specificity against the influenza H5N2 virus by hemagglutination-inhibition (HI) method. The result of this study showed that the dose of 58 mg/chicken/day of aqueous extract of M.citrifolia fruit had an optimal capability to enhance specific antibody (IgY) as well as IgG serum production against avian influenza (H5N2) vaccine .Key words: Adjuvant, extract, Morinda citrifolia, L., H5N2 vaccine, immunoglobulin yolk (IgY)
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/671
10.14499/indonesianjpharm23iss2pp115-120
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 115-120
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/671/881
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1061
2017-09-11T06:04:30Z
3:ART
ANTI-OXIDANT AND ANTI-DIABETIC ACTIVITIES OF ETHANOLIC EXTRACT OF Primula denticulata FLOWERS
Bhatt, Hemlata; Dept Pharmaceutical Sciences, H.N.B. Garhwal University, Srinagar-,Garhwal, Uttarakhand, India. 246174
Saklani, Sarla
Upadhayay, Kumud
The present investigation was carried out to focus on the anti-oxidant and anti-diabetic effect of ethanolic extract of flowers of Primula denticulata by DPPH, ferrous chelating, reducing power assay and streptozotocin induced diabetes respectively. Dose selection was made on the basis of acute oral toxicity study (100mg/kg, 200mg/kg, 400mg/kg bodyweight) as per OECD guidelines 423. The blood glucose levels were measured by using blood glucose measuring strips based on glucose-oxidase method. The extract showed significant anti-oxidant and anti-diabetic activity when compared with standard drug.Keywords: Primula denticulata, anti-oxidant and anti-diabetic activity.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-03-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1061
10.14499/indonesianjpharm27iss2pp74
Indonesian Journal of Pharmacy; Vol 27 No 2, 2016; 74
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1061/772
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1464
2019-10-10T01:30:23Z
3:ART
Citalopram and Tianeptine: Pharmacological Interventions in alcohol withdrawal-induced negative mood states and serotonin insufficiency in Albino Wistar male rats
Amjad, Iffat Ara; Minhaj University Lahore
Abdul Rub, Samina Bano; University of Karachi
Serotonin, Alcohol withdrawal syndrome, Serotonergic antidepressants. Tryptophan metabolism
ABSTRACT The present study aims to investigate the neurochemical and behavioural effects of tianeptine and citalopram to block alcohol withdrawal-induced audiogenic seizures in alcohol withdrawal (AW) rats. Citalopram and tianeptine were administered sub-chronically to block AW-induced audiogenic seizures. Brain regional tryptophan (TRP), 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA) concentrations were determined using high-performance liquid chromatography connected to the fluorimetric detector. Citalopram increased brain TRP concentration in all the regions, however, tianeptine increased brain TRP concentration only in amygdala and hippocampus. Increased brain serotonin concentration was seen by citalopram but not by tianeptine in all the regions. Further, tianeptine was shown to increase 5-HT turnover in all the regions, however, citalopram appeared to increase 5-HT turnover only in the hippocampus. It is concluded that the citalopram and tianeptine behave differently on the intrinsic pathway of serotonin metabolism that appeared to be compensated by the release pattern of serotonin. Further, chronic exposure of serotonergic agents causes restoration and structural reforming of serotonin reuptake mechanism that is desensitized following chronic alcohol exposure. Thus the inclusive approach of the serotonergic system plays an undoubted role in the pharmacological management of AW syndrome.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Higher Education Commission,Pakistan
2019-06-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1464
10.14499/indonesianjpharm30iss2pp75-83
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 75-83
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1464/906
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/656
2020-01-27T02:53:58Z
3:ART
APPLICATION OF FOURIER TRANSFORM INFRARED SPECTROSCOPY FOR QUALITY CONTROL OF PHARMACEUTICAL PRODUCTS: A REVIEW
Rahman, Abdul; Laboratory of Analytical Chemistry, Faculty of
Pharmacy, Universitas Gadjah Mada, 55281,
Yogyakarta, Indonesia. +62274-543120
Analysis of pharmaceutical products covers all aspects of quality control of active pharmaceutical ingredients (API) and finished products. Today, Fourier transform infrared (FTIR)spectroscopy, especially in combination with chemometrics software, has emerged as one of the romising analytical techniques to be used in pharmaceutical industry, for quality control of desired pharmaceutical products. Compared with other instrumental techniques, FTIR spectroscopy offers some advantages, namely it is rapid, simple in sample preparation, and not destructive. In this review, the application of FTIR spectroscopy for qualitative and quantitative determinations of API and monitoring drug release are described.Key words: FTIR spectroscopy, quality control, pharmaceutical products.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/656
10.14499/indonesianjpharm23iss1pp1-8
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 1-8
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/656/522
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1274
2018-10-23T02:18:57Z
3:ART
The Influence of Medication Days’ Supply on Adherence and Cost of Hypertensive Patients at Primary Health Care Centre
Mufarrihah, Mufarrihah; Faculty of Pharmacy, Universitas Airlangga
Setiawan, Catur Dian
Wathaniah, Nurul
Rahmaniah, Atikah
Maharani, Primanda Ayu
Mahmudah, Azzahroh Sifa’ Lailiyah
Achmad, Gusti Noorrizka Veronika
adherence, cost, hypertension, medication days’ supply, Primary Health Care Centre
Primary Health Care Centre is known have policy discrepancy with the supply of medicines. This can influence the adherence and costs incurred by patients. The study aimed to identify the influence for hypertensive patients at Primary Health Care Centre in Surabaya. This study was designed cross sectional and data collection was done by using ARMS questionnaires for the patient's adherence and interview for the cost. Cost was calculated based on the frequency of real visits and monthly patient expenditures. Direct medical cost, direct non medical cost, and indirect cost were included based on patients’ perspective. Direct observations were done to calculate the days of drug supplies. A total of 406 patients from 50 Health Care Centre were included in the study. Days of drug supplies ranged from 3 to 30 days; total ARMS scores ranged from 12 to 33; and the total cost obtained ranged from IDR 34,965 to IDR 239,528. The Spearman’s correlation test sought to obtain the significant influence of medication days’ supply on adherence with p value 0.0001 and r = -0.347. While medication days’ supply didn't show any significant influence on the cost. It will be significant if the patient met the visit schedule, that was p value 0.0001 and r = -0.247. It is recommended to prolong antihypertensive days’ supply in order to improve patients' adherence, as well as to reduce cost expensed by patients.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-12-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1274
10.14499/indonesianjpharm28iss4pp213
Indonesian Journal of Pharmacy; Vol 28 No 4, 2017; 213
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1274/854
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/688
2020-01-27T02:53:31Z
3:ART
PREPARATION AND CHARACTERIZATION OF STAVUDINE ENTRAPPED LIPOSPHERES
Shegokar, Ranjita; C.U. Shah College of Pharmacy, S.N.D.T.
Women’s University, Santacruz (W), Mumbai-400049 India
Singh, Kamalinder; C.U. Shah College of Pharmacy, S.N.D.T.
Women’s University, Santacruz (W), Mumbai-400049 India
Lipospheres of stavudine were prepared by melt dispersion technique using trimyristin, tripalmitin and triastearin, stearic acid, Compritol® 888 ATO and Precirol® ATO 5 as lipid matrix in the various drug-lipid ratios. Drug entrapped free flowing solid lipospheres of triglycerides and glyceryl behenate were characterized for surface morphology, particle size distribution, encapsulation efficiency, and in vitrorelease behavior. The effect of drug lipid ratio, the surfactant used, concentration of stabilizer, and stirring speed were identified as the key variables affecting the formation of discrete spherical lipospheres and sustained drug release rate. The lipospheres production conditions were optimized by using 2% w/w sodium cholate and 1% Plural oleic as a stabilizer. The concentration of lipid used had pronounced effect on particle size of the lipospheres. The incorporation efficiency was found to be in range of 30 to 50%. Increase in concentration of surfactant and stirring speed produced fine spherical, smooth, and round lipospheres. All the prepared lipospheres exhibited slow release profiles dictating the Higuchi mode of release.Key words: Lipospheres, stavudine, lipid matrix, hydrophilic surfactant, drug release
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/688
10.14499/indonesianjpharm23iss4pp231-237
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 231-237
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/688/554
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1156
2017-09-11T06:07:50Z
3:ART
THE IN VITRO ANTIOXIDANT PROPERTIES OF 2-ALKOXYPHENYLCARBAMIC ACID DERIVATIVES CONTAINING A 4´-(SUBSTITUTED PHENYL)PIPERAZIN-1´-YL MOIETY DETERMINED BY THE 2,2´-AZINOBIS(3-ETHYLBENZOTHIAZOLINE-6-SULFONIC ACID) DERIVED RADICAL CATION (ABTS•+) AND FERRIC RE
Malík, Ivan; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Comenius University
Stanzel, Lukáš; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Comenius University
Csöllei, Jozef; Department of Chemical Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences
Čurillová, Jana; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Comenius University
Substituted phenylcarbamates; N-arylpiperazines; antioxidant properties; ABTS•+; FRAP; electronic features; lipophilicity
In an effort to comprehensively characterize an antioxidant profile of 2-alkoxyphenylcarbamic acid-based compounds containing a 4´-(substituted phenyl)piperazin-1´-yl fragment, they were in vitro screened in the 2,2´-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) derived radical cation (ABTS•+) and ferric reducing antioxidant power (FRAP) assay using the UV/VIS spectrophotometry. The ABTS•+ scavenging (reducing) potential of 1-[3-(2-methoxyphenylcarbamoyl)oxy-2-hydroxypropyl]-4-(4-fluorophenyl)piperazin-1-ium chloride was found to be the most promising and it was comparable to the efficiency of the carvedilol reference drug. Moreover, that 4´-fluoro group-containing compound was regarded as more active than the atenolol standard. When testing the molecules´ power to reduce the ferric 2,4,6-tris (2-pyridyl)-s-triazine complex [Fe(III)(TPTZ)2]3+, the most prospective was 1-[3-(2-ethoxyphenylcarbamoyl)oxy-2-hydroxypropyl]-4-(4-fluorophenyl)piperazin-1-ium chloride. On the other hand, its Fe3+ reducing power was lower compared to both standards carvedilol and atenolol. The study discussed structure–antioxidant properties relationships considering electronic, steric and lipophilic features.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-03-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1156
10.14499/indonesianjpharm28iss1pp1
Indonesian Journal of Pharmacy; Vol 28 No 1, 2017; 1
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1156/805
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1698
2022-10-26T01:05:53Z
3:ART
Pharmacoeconomic Rationale of Zinc Supplementation In The Management of Acute Diarrhea In Children With Rotavirus Infection In Indonesia
Hakim, Mohamad Saifudin; Department of Microbiology, Faculty of Medicine, Public Health and Nursing
Soloviov, Serhii
Soenarto, Yati; Department of Chemistry IPB University, Bogor
Dzyublyk, Iryna
Leleka, Maryia
Trokhymchuk, Viktor
iarrhea, modelling, rotavirus, treatment, zinc supplementation
Rotavirus is one of the most important causative agents of gastroenteritis in both infants and children worldwide, resulting in a high mortality and burden of disease, mainly in low-income, developing countries. Oral rehydration therapy with zinc supplementation is currently the recommended way to prevent death from diarrheal dehydration. In this study, we aimed to estimate the effectiveness of zinc supplementation in combination therapy of patients with suspected rotavirus infection in Indonesia. The perspective of this pharmacoeconomic study refer to the economic vantage point of cost-of-illness analysis. Achievement of this goal was possible with the construction of a decision tree model and determination of decision rules for inclusion of zinc supplementation into the combination therapy. The input parameters of the model were hospitalization days of patients stratified by the presence or absence of rotavirus infection as well as the additional inclusion of zinc supplementation. The criterion for prediction and decision making was the global rotavirus prevalence. The feature of the simulation was that the costs were expressed as relative to each other, which allowed to unify the proposed methodology. Retrospective analysis of clinical database of Indonesian patients with acute diarrhea has shown that zinc supplementation would be rational in case of rotavirus prevalence among these patients is higher than 81.5%. It was shown that additional zinc supplementation would be costeffective with probability of 0.62 and 0.53 – in the positive range of cost ratio variation or if costs for less than 43% of baseline therapy per day costs on an average.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Directorate of Research and Community Service, Ministry of Research Technology and Higher Education of the Republic of Indonesia for financial support of this research by Penelitian Fundamental Grant 2019 (No: 4167/IT3.L1/PN/2019).
2020-01-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1698
10.14499/indonesianjpharm30iss4pp285
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 285
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1698/926
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1355
2018-11-02T06:46:53Z
3:ART
The Effect of Ethanol Extract of Piper nigrum L. Fruit on Reproductive System in Adult Male Wistar Rats: A Study of FSH, LH, Testosterone Level and Spermatogenic Cells
Ekaputri, Tia Wida; Master Programme, Basic Medical and Biomedical Science, Faculty of Medicine, Universitas Gadjah Mada, Indonesia
Sari, Ika Puspita; Department of Pharmacology & Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada
Rizal, Dicky Moch.; Department of Physiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Indonesia
black pepper (Piper nigrum L.), FSH, LH, male rat, testosterone, spermatogenic cells
Exploration to find out new natural contraceptive agent for male is still being developed. Black pepper (Piper nigrum L.) and its main alkaloid piperine have potential antifertility because of sitotoxic and hormonal effects. The aim of this study is to find out the effect of ethanolic extract of black pepper (Piper nigrum L.) fruit of on reproductive hormone serum level, sperm quality, and spermatogenic cell populations in adult male Wistar rat. Twenty five male rats were divided into five groups consisting of two control group, i.e. K(-) (Na-CMC 0.5%), K(+) (finasteride 0.45mg/kg BW), and three groups received different doses of black pepper fruit ethanolic extract, i.e. D(1) (3.33mg/kg BW), D(2) (6.66mg/kg BW) and D(3) (13.32mg/kg BW) respectively. The treatment were given to each group for 55 days. Reproductive parameters were measured, including serum level of reproductive hormones (FSH, LH, dan testosteron), quality of cauda epididymal sperm (spermatozoa concentration, motility and morphological abnormality), spermatogenic cell populations (primary spermato-cyte and spermatid count) and seminiferous tubules diameter. Ethanol extract of black pepper fruit at doses of 3.33mg/kg BW, 6.66mg/kg BW, and 13.32mg/kg BW increased serum FSH level. Extract at dose of 13.32mg/kg BW decreased serum LH level, while extract at doses of 6.66mg/kg BW and 13.32mg/kg BW decreased serum testosterone level. The number of primary spermatocytes, spermatozoa concentration, and spermatozoa motility were decreased by administration of ethanol extract of black pepper fruit with dose of 6.66mg/kg BW and 13.32mg/kg BW. Ethanol extract of black pepper fruit at dose of 6.66mg/kg BW and 13.32mg/kg BW had a negative impact on the male reproductive system and showing potential antifertility in male rat.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-09-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1355
10.14499/indonesianjpharm29iss3pp136
Indonesian Journal of Pharmacy; Vol 29 No 3, 2018; 136
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1355/875
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1325
2018-11-02T06:46:53Z
3:ART
Free radical scavenging potential of Drosera indica L in presence of Dalton Ascites lymphoma (DAL) tumor bearing mice
Asirvatam, Raju
Christina, AJM
: Drosera indica L, Antioxidant, Dalton Ascites lymphoma, Malondialdehyde, Protein carbonyl content
The aim of the present study was to evaluate the antioxidant role of Drosera indica L. in Dalton Ascites lymphoma (DAL) bearing Swiss albino mice. The effect of ethanol and aqueous extracts of D.indica (EEDI and AEDI) were administered at a 250 and 500mg/kg once a day for 14 days, 24h after the inoculation of tumor cell line. After the treatment period, blood was collected from the animals and subsequently the animal were sacrificed for isolation of liver, brain, kidney and lungs for the observation of antioxidant status level. The parameters analyzed were catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), peroxidase (P), total protein (TP) and protein carbonyl content (PCC). Treatment with EEDI and AEDI significantly reduced the levels of MDA and increased the levels of GSH, SOD, CAT, P and TP in cancer induced animal and are similar as that of normal mice. The results suggest that the ethanol and aqueous extract of Drosera indica L. possess significant antioxidant effects in DAL bearing mice
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-07-27
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1325
10.14499/indonesianjpharm29iss3pp127
Indonesian Journal of Pharmacy; Vol 29 No 3, 2018; 127
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1325/874
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1023
2017-09-11T04:59:09Z
3:ART
STRUCTURE–ANTIMICROBIAL PROPERTIES STUDY OF SOME DIBASIC PHENYLCARBAMIC ACID ESTERS
Malík, Ivan; Dept. of Pharmaceutical Chemistry, Faculty of Pharmacy, Comenius University in Bratislava, Slovak Republic
Csöllei, Jozef; Dept of Chemical Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences in Brno, Czech Republic
Bukovský, Marián; Dept. of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, Slovak Republic
Due to worldwide phenomenon of microbial resistance to commonly used therapeutic agents, antibiotics and antifungals, dibasic di‑/trimethylphenylcarbamic acid esters 1–3, a non-traditional series of potential antimicrobials, has been in vitro evaluated against chosen Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacterial strains as well as against yeast (C. albicans) by the minimum inhibitory concentration (MIC) assay. Taking into consideration chemical structure of tested derivatives, the incorporation of more than one protonated atom of nitrogen into salt forming fragment positively influenced the activity against E. coli. On the contrary, the presence of one or more methyl groups instead of 3-alkoxy side chain attached to lipophilic aromatic moiety has not found to be favorable structural feature. In entire set of inspected compounds, the most promising results have been found for the compound 3, chemically1-[3-piperidinium-1-yl-2-({[(2,4,6-trimethylphenyl)amino]carbonyl}oxy)propyl]piperidinium dichloride, against E. coli with the MIC=1.56 mg/mL. Key words: Dibasic phenylcarbamic acid esters, Escherichia coli, hydrogen bonding, lipophilicity
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1023
10.14499/indonesianjpharm27iss1pp53
Indonesian Journal of Pharmacy; Vol 27 No 1, 2016; 53
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1023/768
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1417
2019-10-10T01:29:33Z
3:ART
Nanocurcumin Preparation for Reducing Vcam-1 and IL-6 in High Fat Diet-Induced Hyperlipidemic Rats
Pradana, Dimas Adhi; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia Jl. Kaliurang, km. 14,5 Yogyakarta 55584 Indonesia
Ardhi, Maulana; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia Jl. Kaliurang, km. 14,5 Yogyakarta 55584 Indonesia
Hasyono, Arifa Caryn Dea Utami; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia Jl. Kaliurang, km. 14,5 Yogyakarta 55584 Indonesia
Meytasari, Dzihni; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia Jl. Kaliurang, km. 14,5 Yogyakarta 55584 Indonesia
Nabilah, Farah Deastasa
Istikharah, Rochmy
Chabib, Lutfi
Atherosclerosis, curcumin, SNEDDS, nanosuspension, nanoemulsion, solid lipid nano, VCAM-1, IL-6, preventive measure
Hyperlipidemia is a pathological condition due to lipid metabolism abnormalities. Increased and oxidized LDL in hyperlipidemia will trigger an inflammatory response and produce proinflammatory cytokines, such as vascular cell adhesion molecule-1 (VCAM-1) and Interleukin-6 (IL-6). Curcumin can be used as antioxidants, anti-inflammatory and antihyperlipidemia, but as it is practically insoluble in water, formulation of curcumin nanosuspension is made to improve the effects of curcumin therapy. This study was conducted to determine the activities of nanocurcumin preparation as preventive measures for rats induced with hyperlipidemia. The test animals used were 49 male Wistar rats divided into 7 test groups: normal control group, negative control group, 80mg/kg BW/day curcumin control group, and nanosuspension curcumin dose of 80mg/kg BW/day, nanoemulsion curcumin dose of 80mg/kg BW/day, SNEDDS curcumin dose of 80mg/kg BW/day and solid lipid nanoparticles dose of 80mg/kg BW/day. Provision of preventive measure was performed on days 1-67. On day 11-67, the induction of lard and egg yolk (1:1) was administered with a volume of 2mL/200g of rat BW. On the 68th day, blood samples were taken for the determination of VCAM-1 and IL-6 parameters using Biotin-Streptavidin-Amplified Enzyme-Linked Immuno-sorbent Assay method. Data analysis of VCAM-1 and IL-6 levels between test groups were conducted by means of normality test and One-way ANOVA (p<0.05). Based on the data of VCAM-1 and IL-6 levels, nanocurcumin dose of 80mg/kg BW preparation was able to significantly improve the preventive activity of curcumin compared to curcumin suspension dose of 80mg/kg BW by reducing VCAM-1 levels in SNEDDS (53.260%), nanoemulsion (52.737%), nanosuspension (52.325%) and solid lipid nanoparticles (51.444%) and decreasing IL-6 levels in SNEDDS (33.030%), nanoemulsion (31.568%), nanosuspension (29.898%), and solid lipid nanoparticles (28.875%).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Directorate of Research and Community Service of the Universitas Islam Indonesia, Laboratory of Pharmacology and Laboratory of Pharmaceutical Technology Department of Pharmacy Universitas Islam Indonesia
2019-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1417
10.14499/indonesianjpharm30iss1pp58
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 58
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1417/894
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1183
2017-08-30T06:55:29Z
3:ART
SYNTHESIS AND CYTOTOXIC ACTIVITY OF 2,5-BIS(4-BORONIC ACID)BENZYLIDINE CYCLOPENTANONE ON HER2 OVEREXPRESSED-CANCER CELLS
Utomo, Rohmad Yudi
Putri, Herwandhani
Pudjono, Pudjono
Susidarti, Ratna Asmah
Jenie, Riris Istighfari
Meiyanto, Edy
Synthesis of PGB-0; MCF7/HER-2; cytotoxic
Development of chemotherapeutic agent and boron carrying pharmaceutical based on HER2 specific targeted is important due to its role in enhancing cancer progression. The purpose of this study is to synthesize curcumin analogue, namely Pentagamaboron-0 (PGB-0) or 2,5-bis(4-boronic acid)benzylidine cyclopentanone, and to explore the cytotoxic activity on HER2 overexpressed-cancer cells. MCF-7/HER2 was used as a model of HER2 overexpressed-cancer cells and NIH3T3 as normal cells. PGB-0 bound to ATP binding site of HER2 and EGFR based on molecular docking study. PGB-0 was synthesized resulting in 33% yield and was confirmed by IR, 1HNMR, 13CNMR and Mass spectroscopy. Based on MTT assay PGB-0 decreased cells viability on MCF-7/HER2 cells with IC50 value of 270 µM but performed no effect on NIH3T3 cells. Cell cycle analysis revealed that PGB-0 increased sub-G1 accumulation. PGB-0 decreased HER2 expression in a dose-dependent manner. We conclude that the new compound PGB-0 inhibits cell growth through cell death induction and decreased HER2 expression. Thus, PGB-0 is potential to be developed as a chemotherapeutic agent and boron carrying pharmaceutical targeted on the HER2 receptor.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-08-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1183
10.14499/indonesianjpharm28iss2pp74
Indonesian Journal of Pharmacy; Vol 28 No 2, 2017; 74
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1183/813
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1367
2019-10-03T03:16:50Z
3:ART
α-Glucosidase Inhibition Activity of Stem Bark Ceiba pentandra Linn. In Vitro and In Silico
Syihabudin, Vicri; Pakuan University
α-glucosidase; AutoDock Vina; LCMS/MS; vavain derivative
Ceiba pentandra (L.) stem bark plant contains tannin, flavonoid, alkaloid, terpenoid, and saponin compounds. It is known that flavonoid compounds have a potential for inhibition activity of an α-glucosidase enzyme. The purpose of this research is to know the α-glucosidase inhibition activity of the fraction of n-hexane, ethyl acetate, and water. These three fractions measured α-glucosidase inhibition activity by spectrophotometric UV-vis method. The fraction extract with IC50 values can best be done an analysis of active compounds using LCMS/MS methods, and results of the suspected compound do the binding molecule with a receptor model isomaltase α-glucosidase uses AutoDock Vina so that bond can predict drug active compounds. The results of this research activity are inhibition of α-glucosidase stem bark in the fraction of n-hexane 4,60 μg/mL, ethyl acetate fraction 8,55 μg/mL and fractions of the water 5,61 μg/mL. Analysis of the compound fraction of water by LCMS/MS eluted at the retention time 3,61 seconds allegedly derivative compounds (+) catechin and on retention time 8,70 minutes allegedly derivative compounds vavain. Vavain derivative compounds, (+) catechin, acarbose and quercetin each has its own docking score of -8,1; -8,8; -6,2 and -7,6 kcal/mol and has a semblance of bonding on amino acid Glu276 which is a residue catalytic role in hydrolysis reactions. Equality on the sides of active chemical bonds is expected to have the same activity as drug inhibition of the enzyme α-glucosidase.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Bina Lohita Sari, Novi Fajar Utami, Purnama Alam and Utari
2018-12-17
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1367
10.14499/indonesianjpharm29iss4pp206
Indonesian Journal of Pharmacy; Vol 29 No 4, 2018; 206
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1367/885
Copyright (c) 2018 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1439
2019-10-10T01:30:23Z
3:ART
Immunomodulatory Activity of Yogurt Fortified with Honey and Rosella (Hibiscus sabdariffa L.) on Reactive Oxygen Intermediate (ROI) and Nitric Oxide (NO) Secretion
Mahfudh, Nurkhasanah; Faculty of Pharmacy, Universitas Ahmad Dahlan
Novitasari, Rina; Faculty of Pharmacy, Universitas Ahmad Dahlan
Immunomodulator, Yoghurt, Rosella (Hibiscus sabdariffa L), ROI, NO
Raise the body's immune system. Rosella (Hibiscus sabdariffa Linn.) is known to have anthocyanin compounds that have antioxidant and immunomodulatory effects. The aim of this research is to know the effect of immunomodulator yogurt which fortified by rosella (Hibiscus sabdariffa L.) extract to increase the secretion of Reactive Oxygen Intermediate (ROI) and Nitric Oxide (NO). This research was conducted in vivo using 25 male mices with Balb/C strain divided into 5 groups: normal group, plain yogurt treated group and 2%, 4%, 8% rosella yogurt treated group. Treatment was given for 21 days orally. On the 22nd day the mice were sacrificed by taking peritoneum macrophage cells and then tested the secretion of Reactive Oxygen Intermediate (ROI) and Nitrite Oxide (NO). The results showed that there was an increase of ROI and NO secretion in 2%, 4%, and 8% rosella yoghurt treated groups compare to plain yoghurt. The fortification of yoghurt with rosella extract and honey increase the potency of yoghurt in increasing the immunomodulatory activity
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-05-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1439
10.14499/indonesianjpharm30iss2pp141-146
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 141-146
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1439/901
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/815
2017-09-12T06:56:09Z
3:ART
MUTATION INDUCTION FOR IMPROVING OF ARTEMISININ CONTENT IN EACH PART OF ARTEMISIA CINA MUTAN LINES
Aryanti, Aryanti; Center for Isotopes and Radiation Application ( CIRA/PAIR ), NEA/BATAN
Jl. Lebak Bulus Raya No. 49
Jakarta Selatan, Indonesia
Yuniawati, Marina; Center for Isotopes and Radiation Application ( CIRA/PAIR ), NEA/BATAN
Jl. Lebak Bulus Raya No. 49
Jakarta Selatan, Indonesia
Mutation induction on Artemisia cina herbal medicine by gamma rays with the doses of 10, 20 and 40 Gy have been conducted at PAIR – BATAN. The purpose of mutation for improving of plant traits which were mutant lines more artemisinin content than control plant. The mutant lines interest were collected based on morphological characters (flowers, leaves, roots and stems). Artemisinin contents so far are only found in the leaves, the improvement are expected not only to increase artemisinin content in leaves but also in the roots, stems and flowers of A.cina mutant lines by mutation induction using gamma rays by the doses of 10, 20 and 40 Gy, every dose was selected 8 mutant lines for analysis artemisinin content.To obtain artemisinin from all selected mutant lines, flowers, leaves, roots and stems were extracted by using n-hexan, and then fractionation by ethyl acetate. Artemisinin content were analyzed by High Pressure Liquid Chromatography (HPLC), and pure artemisinin used as standard. Mutation induction by the dose of 40 Gy have been improving dry weight of roots from 38.85 to 76.19g, dry weight of leaves of mutant lines from 10 Gy irradiation was two times higher than leaves of parent plant, and three times by the dose of 40 Gy compared to dry weight of leaves parent plant, and no different by the dose of 20 Gy. The highest artemisinin content is 73.13mg/g in leaves of A26a3 mutant line and 36.68mg/g in flowers of A17.1 A.cina mutant lines, both of mutant lines from mutation induction by the dose of 10 Gy. Mutation induction by the dose of 20 Gy could improve artemisinin content in stems part of B12.1 mutant line from non detection in parent plant to 0.83mg/g. Mutation induction by the dose of 40 Gy improved artemisinin content to 1.90mg/g in roots and 1.50mg/g in stems of C27b1 and C8.3 mutant lines respectively. Artemisia cina un-irradiated as parent plant only contain artemisinin in flowers, leaves and roots were 0.13; 0.45 and 0.05mg/g respectively. Dose of 40 Gy is the best dose for enhancement of artemisinin content in each part of A.cina mutant lines.Key word : mutation, production of artemisinin, roots, stems, flowers
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2015-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/815
10.14499/indonesianjpharm25iss3pp158
Indonesian Journal of Pharmacy; Vol 26 No 3, 2015; 158
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/815/746
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1087
2017-11-07T00:57:21Z
3:ART
MONOSODIUM GLUTAMATE EXPOSURE AT EARLY DEVELOPMENTAL STAGE INCREASES APOPTOSIS AND STEREOTYPIC BEHAVIOR RISKS ON ZEBRAFISH (DANIO RERIO) LARVAE
Kurnianingsih, Nia; Departement of Physiology, Faculty of Medicine, Brawijaya University
Utami, Juliyatin Putri; Biomedical Science, Faculty of Medicine, Universitas Brawijaya
Nurdiana, Nurdiana; Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya
Lyrawati, Diana; Department of Pharmacy, Faculty of Medicine, Universitas Brawijaya
Monosodium Glutamate; Early Embryo; Duration; Apoptosis; Behavior
Excessive glutamate may give neurotoxic effects and contribute to Autism spectrum disorder(ASD). In this study, we investigated prolonged exposure effects of 10 µg/mL Monosodium Glutamate (MSG) on intracellular calcium level, bax, bcl-2, ratio of bax/bcl-2 genes expression, caspase-3, apoptosis of brain cells and stereotypic behavior of Zebrafish (Danio rerio) larvae at early developmental stages. Genes expression were determined by real time PCR, caspase-3 using ELISA, intracellular Ca2+ and apoptotic cells of brain using confocal microscopy, locomotor activity by using crossing lines assay whereas stereotypic behavior by circle swimming. The results indicated that MSG exposure increased brain bax and bcl-2; and caspase-3; intracellular Ca2+; and apoptosis; stereotypic behavior; and decreased locomotor activity. Termination of MSG treatments resulted in recovery of bax, bcl-2, caspase-3 basal levels and stereotypic behavior. In conclusion, MSG exposure at early embryonic stage increased brain cell damage and risk of behavior changes.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1087
10.14499/indonesianjpharm27iss3pp128
Indonesian Journal of Pharmacy; Vol 27 No 3, 2016; 128
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1087/787
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1020
2017-08-25T03:51:50Z
3:ART
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/683
2020-01-27T02:53:31Z
3:ART
REVIEW OF BIOLOGICAL ACTIVITIES OF HYDRAZONES
Ali, Md. Rahmat; Department of Pharmaceutical Chemistry,
Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
Marella, Akranth; Department of Pharmaceutical Chemistry,
Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
Alam, Md. Tauquir; Department of Pharmaceutical Chemistry,
Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
Naz, Ruksar; Department of Pharmaceutical Chemistry,
Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
Akhter, Mymoona; Department of Pharmaceutical Chemistry,
Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
Shaquiquzzaman, Md.; Department of Pharmaceutical Chemistry,
Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
Saha, Rikta; Department of Pharmaceutical Chemistry,
Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
Tanwar, Omprakash; Department of Pharmaceutical Chemistry,
Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
Alam, Md. Mumtaz; Department of Pharmaceutical Chemistry,
Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
Hooda, Jyoti; CHRD- Competitive and Scientific Intelligence
(CSI), WNS Global Services (P) Ltd, Gurgaon
122002, India.
Hydrazones possess an azomethine –NHN=CH group and are considered as derivatives of aldehydes and ketones in which the oxygen atom has been replaced by the NNH2 functional group. These are widely studied molecules owing to their ease of preparation and diverse pharmacological potential. This has led researchers to synthesize different heterocyclic compounds bearing hydrazones. Medicinal chemists across the world have done immense work on hydrazones and developed agents with better activity and low toxicity profiles. Following different synthetic protocols and through proper SAR studies differently substituted hydrazones have been developed and found to be active against different pharmcological targets. They are known to possess different biological activities viz. antimicrobial, antiinflammatory, anticancer, antimalarial etc. These observations have been guiding for the development of new hydrazones that possess varied biological activities. The review aims at highlighting the diverse biological activities of hydrazones.Key words: Azomethine, Hydrazones, Pharmacological Potential
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/683
10.14499/indonesianjpharm23iss4pp193-202
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 193-202
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/683/549
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/988
2017-09-11T06:11:43Z
3:ART
COMPARATIVE STANDARDIZATION OF MARKETED FORMULATIONS OF FERMENTED BIOMEDICINE – ARJUNARISTHA
Randive, Dheeraj Suhas; assitant prof. dept of pharmaceutics RCP KASEGAON, TAL- WALAWA, DIST- SANGLI, MAHARASHTRA, (INDIA) 415404
Ayurveda; Arjunarishta; Marketed formulation; Thin layer chromatography
Ayurvedic formulations have proved to be effective in the prevention and treatment of many life-threatening diseases. Asavas and Arishtas have been used as medicine for over 3000 years as appetizer and stimulant. In the present study 6 different marketed brands (Two having different batches) of Arjunarishta were thoroughly evaluated for their organoleptic characteristics and physicochemical parameters, to establish a routine procedure for standardization of these Ayurvedic formulations. The organoleptic tests performed include colour, odour and taste whereas the physicochemical parameters evaluated were pH, Refractive index, Specific gravity, Viscosity, density, surface tension, Water-soluble extractive, Alcohol-soluble extractive Acid value, Alcohol content, by distillation and dichromate oxidation method, Total solid content, Total phenol content, In present communication, a TLC method was developed for the evaluation of Arjunarishta by quantitative estimation of major compound gallic acid and ellagic acid.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-12-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/988
10.14499/indonesianjpharm27iss4pp220
Indonesian Journal of Pharmacy; Vol 27 No 4, 2016; 220
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/988/796
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/988/78
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1492
2022-10-26T01:05:53Z
3:ART
Quantitative Analysis of Multi-components in Curcuma xanthorrhiza by Single Marker
Badrunanto, Badrunanto
Wahyuni, Wulan Tri
Nirwati, Hera
Rafi, Mohamad
C. xanthorrhiza; curcuminoids; QAMS; relative coefficient factor
A new simple and effective routine analytical method for quantification of curcuminoids in Curcuma xanthorrhiza was developed by high-performance liquid chromatography. This method based on chromatographic fingerprint combined with a quantitative analysis of multi-components by single marker (QAMS). Curcumin was selected as an internal marker for the determination of two other similar compounds, i.e. bisdemethoxycurcumin and demethoxycurcumin, by using the relative coefficient factor (RCF). Excellent linearity was obtained for each component (r2 > 0.9998), and the recovery of extraction methods were within 100.23-103.95%. The precision of the method was good at inter-day and intra-day analysis (RSD < 4.0%). The stability of RCFs was good under various chromatographic conditions with RSD < 1%, and the ratio of retention time was used to locate each compound. The quantification of curcuminoids between QAMS and external standard method (ESM) proved the consistency and similarity of the two method (RSD < 2%). This study demonstrated that QAMS could be used as a routine method for quality control of curcuminoids in C. xanthorrhiza. This method successfully proved accurate, stable, more effective and simple than external standard method.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-10-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1492
10.14499/indonesianjpharm30iss4pp301
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 301
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1492/921
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/507
2020-02-19T02:37:07Z
3:ART
SEDATIVE EFFECT OF CLARY SAGE (SALVIA SCLALAREA, L) ON MICE USING AN OLFACTORY AROMATHERAPY
Hadinoto, Idajani; Faculty of Pharmacy
Unika Widyamandala Surabaya
Kuswono, Engkun; Faculty of Pharmacy
Unika Widyamandala Surabaya
Marlina, Ani; Faculty of Pharmacy
Unika Widyamandala Surabaya
Setiawati, Anna; Faculty of Pharmacy
Unika Widyamandala Surabaya
A research has been conducted to study the sedative effect of the Clary sage oil (Salvia sclaria, L), using an olfactory aromatherapy method, on male white mice, which have been tested using rotarod, platform and chimney tests. Clary sage oil was given to the male white mice in three different exposed times : 0.5, 1, and 1.5 hours. As a reference standard was used diazepam 170 ug/kg body weight (intraperitoneal). The research indicates that the administration of Clary sage oil can indeed cause the sedative effect. There is also a correlation between the exposure time of Clary sage oil and the sedative effect observed. Key-words : sedative effect, clary sage oil, olfactory aromatherapy
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/507
10.14499/indonesianjpharm0iss0pp44-51
Indonesian Journal of Pharmacy; Vol 12 No 1, 2001; 44-51
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/507/385
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1308
2018-10-23T02:19:33Z
3:ART
Protective Effect of Ethanolic Extract Tempuyung Leaf (Sonchus arvensis L.) in Gentamicin-induced Acute Tubular Necrosis on Wistar Rats
Imelda, Imelda -; Biomedical Science Master Program, Graduate School of Medical Sciences, Universitas Padjadjaran, Bandung
Achadiyani, Achadiyani -; Department of Anatomy, Physiology and Cell Biology,Faculty of Medicine, Universitas Padjadjaran, Bandung
Sekarwana, Nanan -; Department of Pediatrics, Faculty of Medicine, UNPAD-RSHS, Bandung
acute tubular necrosis, creatinine, gentamicin, tempuyung, Sonchus arvensis L.
Acute tubular necrosis (ATN) is the most common histopathologic findings of acute kidney injury (AKI). AKI is marked by the decrement of glomerular filtration rate, causing waste metabolism retention (creatinine). Gentamicin is used as it is the most common nephrotoxic agent in inducing ATN. Tempuyung (Sonchus arvensis L.) has been used in folklore medicine to ameliorate kidney problems as it contains antioxidants, two of which are flavonoid and phenolic acid. Yet, these active substances’ benefit on gentamicin induced ATN has not been investigated in Indonesia. This research aims to analyze protective effect of ethanolic extract tempuyung leaf (EET) on gentamicin-induced ATN based on histopathological study and creatinine serum level. True experimental laboratoric study was done with simple random design on male wistar rats, randomly divided into 4 groups (n=4). Control group (CMC 0.5% aquadest); Induction group (Gentamicin 80 mg/kgBW); 1st treatment group (EET 100 mg/kgBW + Gentamicin 80 mg/kgBW) and 2nd treatment group (EET 200 mg/kgBW + Gentamicin 80 mg/kgBW) for ten days. On the 11th day, blood was taken for creatinine measurement and kidneys collection for histopathological study. Histopathological examination on gentamicin-treated rats revealed degenerative changes in kidney tubules. Aside from that, gentamicin-treated rats also showed increment in creatinine serum level. Conversely, simultaneous administration of EET with Gentamicin ameliorated the nephrotoxic effects of gentamicin as confirmed from the significant improvement on histopathological changes and normalization of creatinine serum level. Co-administration of EET and gentamicin provides protection on gentamicin-induced ATN, based on histopathological feature and creatinine serum level.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
-
2018-05-11
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1308
10.14499/indonesianjpharm29iss2pp86
Indonesian Journal of Pharmacy; Vol 29 No 2, 2018; 86
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1308/870
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1308/142
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1308/143
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1308/144
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/954
2017-09-11T04:57:50Z
3:ART
ANALYSIS OF ACTIVE COMPOUNDS IN BLOOD PLASMA OF MICE AFTER INHALATION OF CAJUPUT ESSENTIAL OIL (MELALEUCA LEUCADENDRON L.)
Muchtaridi, Muchtaridi; Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Jatinangor KM 21-Sumedang.
Tjiraresmi, Ami; Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Jatinangor KM 21-Sumedang.
Febriyanti, Riska; Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Jatinangor KM 21-Sumedang.
The research is intended for the determination of volatile compounds of cajuput oil (Melaleuca leucadendron L.) in a group of mice (3 mice) after inhaling 1 mL cajuput oil for 1/2, 1 and 2 hour(s). The blood plasma of three mice was collected in heparin tube, and the volatile compounds were isolated and concentrated using C-18 Solid Phase Extraction (SPE) column with methanol and bidistilled water mixture (60:40) as the solvent. The analysis was carried out by GC/MS. The results showed that there were 52 compounds in the cajuput oil, especially 1,8-cineole,a-terpineol, b-pinene, a-pinene, b-myrcene, 4-terpineol, linalool, g-terpinene, and terpinolene. The major compound identified in blood plasma of mice was 1,8-cineole. Half an hour after 1 mL inhalation of cajuput oil per mouse, the concentration of 1,8-cineole identified was 41.5 mg/mL, while 1 and 2 hours after 1 mL inhalation of cajuput oil, the level of 1,8-cineole in blood plasma of 3 mice was 646.9 mg/mL and 138.0 mg/mL in blood plasma of each mouse. Other compounds identified were a-terpineol, 4-terpineol linalool, terpenyl acetate and hydrocarbon terpenes. Concentrations of target compounds in the blood plasma were relatively in low level (mg/mL). In conclusion, the volatile compounds of 4-terpineol, linalool, a-terpineol and 1,8-cineol that identified in blood plasma of mice after inhalation of the cajuput oils might correlate with depressive characteristics of essential oil given by inhalation.Keywords: Cajuput, Essential Oils, Inhalation, SPE, GC-MS
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/954
10.14499/indonesianjpharm26iss4pp219
Indonesian Journal of Pharmacy; Vol 26 No 4, 2015; 219
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/954/758
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1203
2018-01-09T08:39:22Z
3:ART
Chemical composition of rhizome oleoresin and anti-inflammatory, antinociceptive and antipyretic activity of oleoresins of Alpinia allughas Roscoe. from tarai region of Uttarakhand
Kumar, Ravendra; Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar, U.S. Nagar, Uttarakhand, India
Sethi, Sonali; Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar, U.S. Nagar, Uttarakhand, India
Prakash, Om; Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar, U.S. Nagar, Uttarakhand, India
Pant, Anil Kumar; Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar, U.S. Nagar, Uttarakhand, India
Kumar, Mahesh; Department of Vet. Epidemiology and Preventive Medicine, College of Veterinary & Animal Sciences. G.B. Pant University of Agriculture & Technology, Pantnagar, U.S. Nagar, Uttarakhand, India
Isidorov, Valery A.; Institute of Chemistry, Białystok University, Division of Environmental Chemistry, UL. Hurtowa 1,15-399, Białystok, Poland
Szczepaniak, Lech; Institute of Chemistry, Białystok University, Division of Environmental Chemistry, UL. Hurtowa 1,15-399, Białystok, Poland
ABSTRACT The investigation of volatile constituents of the rhizome oleoresin of Alpinia allughas Roscoe. growing in tarai region of Kumaun hills, Uttarakhand, revealed the presence of 55 terpenoids. The major constituents identified in the rhizome oleoresin were α-eudesmol (21.3%), β-selinene (13.9%), valencene (9.6%), borneol (7.1%), α-humulene (5.3%) and 7-epi-α-selinene (5.2%). Other minor constituents identified were (E)-β-caryophyllene (3.8%), (6 E)-nerolidol (3.5%), (E,E)-farnesol (3.2%), caryophyllene oxide (2.5%), humulene oxide (2.7%), bornyl acetate (1.9%), coranarin- E (1.8%), linalool (1.8%) and α-terpineol (1.6%). The total identified constituents contribute 97.0% of the oleoresin. The rhizomes oleoresins exhibited significant antinociceptive activity with 34.79% inhibition at 50 mg/kg body weight and 43.24% at 100 mg/kg body wt. compare to standard drug ibuprofen (40 mg/kg body wt.), it also showed antipyretic activity in dose dependent manner with temperature reduction 77.57±5.88% at 50 mg/kg body wt. and 98.95±3.95% at 100 mg/kg body wt. after 3 hours. Oleoresin also showed 29.23% inhibition in carrageenin-induced paw edema at 50 mg/kg body wt. and 39.92% inhibition at 100mg/kg body wt. in compare to ibuprofen 40.06% at 40 mg/kg body weight.Keywords Alpinia allughas Roscoe.; Zingiberaceae; α-eudesmol; β-selinene; anti-inflammatory; antinociceptive; antipyretic activity; oleoresins
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
UGC, NEW DELHI, INDIA
2017-08-16
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1203
10.14499/indonesianjpharm28iss3pp136
Indonesian Journal of Pharmacy; Vol 28 No 3, 2017; 136
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1203/831
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1203/119
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1691
2021-01-06T13:13:53Z
3:ART
An Assessment of Cough Medicine Dispensing Practice to Children Under Two Years Old in Pharmacies in Ho Chi Minh City Using Simulated-Patient Method
Phan, Quyen Thi Ngoc; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Truong, Van Thi; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Pham, Thanh Ngoc; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Nguyen, Ho Nhu; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Department of Pharmacy, Nguyen Trai Hospital, Vietnam
Bui, Quynh Thi Huong; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Department of Pharmacy, Thong Nhat Hospital, Vietnam
Cough medications, Children, Pharmacy
Over-the-counter (OTC) cough and cold medications (CCMs) have been used to treat the symptoms of upper respiratory infection in children for decades. The safety of CCMs in children has been questioned. The data on knowledge of pharmacists in supplying cough medicines for children under two years have been limited. This study aimed to evaluate the pharmacists’ dispensing decisions to manage the cough in children under two years old. A descriptive cross-sectional was carried out in 300 pharmacies in 15 districts in Ho Chi Minh City, Vietnam. The pharmacists were interviewed by a simulated patient. The results showed that, information that pharmacists actively asked the client about the patient and disease symptoms was limited. Most pharmacists did not provide adequate instructions and counsel about using drugs for clients. Only 22/300 (7.33%) of pharmacists appropriately provided cough medicines for children under 2 years old. The main reason of inappropriateness was the deficiency of knowledge about updated contraindication of N-acetylcysteine (93.17%). Pharmacists in pharmacies located in districts 3, 11 and Binh Thanh had higher rate of rational provision than those in other districts. A good and full understanding of the patient symptom helped the pharmacists supply cough medicines more reasonably. The limited caution of pharmacists and the low proportion of pharmacists updating contraindication of N-acetylcysteine should be considered as a warning sign in pharmacy practice in Ho Chi Minh City, Vietnam.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-04-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1691
10.14499/indonesianjpharm31iss1pp42
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 42
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1691/943
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1691/265
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/666
2020-01-27T02:51:49Z
3:ART
BIOLOGICAL IMPORTANCE AND HEALTH EFFECT OF Perilla frutescence PLANT
Asif, Mohammad; Department of pharmacy, GRD (PG) IMT, Dehradun, Uttarakhand, India
The study was designed to study the Biological importance and nutritional benefits of Perilla frutescence plant. This plant has very high biological activities. It contains various phytoconstituents such as different phenolic compounds (like rosemarinic acids, luteolin chrysoeriol, quercetin, catcehin, apigenin, etc), vitamins, minerals etc. Perilla seeds contained 30-40% fixed oil, contained both saturated (palmitic acid 5-7%, stearic acid 1-3%), unsaturated (UFAs) or polyunsaturated (PUFAs) (oleic acid 12-22%, linoleic acid 13-20%, γ-linolenic acid 0-1%, α-linolenic acid 52-64%, icosanoic acid 0-1%) fatty acids. Perilla oil is highly unsaturated oil among the all current discovered natural vegetable oils. Whole perilla plant has many important functions on improving and accelerating human health and is effective in prevention of various diseases.Key words: Perilla frutescence, biological activities, phyto-constituents, polyphenols, polyunsatured fatty acids.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/666
10.14499/indonesianjpharm23iss2pp84-92
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 84-92
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/666/532
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1512
2019-10-10T01:29:33Z
3:ART
Conjugation of Anti-EpCAM Antibody on Alginate–RIP MJ-30 Nanoparticle through Carbodiimide Reaction as a Model of Targeted Protein Therapy
Ismail, Hilda; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia – 55284
Ciptasari, Ummi H.; Undergraduate Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia - 55284
Ikhsan, M Arief Nur; Undergraduate Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia - 55284
Suryani, Fidya; Undergraduate Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia - 55284
Sismindari, Sismindari; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia – 55284
Martien, Ronny; Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia – 55284
Yuswanto, Ag; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia – 55284
Ribosome inactivating proteins from Mirabilis jalapa L. (RIP MJ) has shown higher cytotoxic activity when being formulated as a nanoparticle. However, the selectivity of the delivery system is also an important aspect when it comes to cytotoxic cell therapy. Epithelial cell adhesion molecule (EpCAM) is a monomeric glycoprotein which is overexpressed in epithelial cancer cells. This study aim was to develop a model of targeted protein delivery system by formulating the base fraction of RIP MJ (RIP MJ-30) into alginate nanoparticles and conjugating it with anti-EpCAM antibody. RIP MJ-30 was formulated in to nanoparticle using alginate and CaCl2 as cross-linker. Optimization of conjugation reaction condition was done in the pH variation of 4.5, 5.5, and 6.5. The success of conjugation was analyzed qualitatively using native polyacrylamide gel electrophoresis (native-PAGE) method and BCA assay. The optimum formula of RIP MJ-30 nanoparticles was produced using 0.3% alginate and 0.2% CaCl2. Results indicated that optimum conjugation reaction was carried out at pH level of 5.5. The optimum native-PAGE condition was by using 8% polyacrylamide gel in duration of 6h. Characterization of nanoparticle resulted in particle size of 205.0nm, zeta potential of -6.9mV, entrapment efficiency of 71.11±4.84%, and conjugation efficiency of 89.55±6.18%. It was concluded that RIP MJ-30 was successfully formulated into alginate nanoparticle and conjugated to anti-EpCAM antibody through carbodiimide reaction using 1-ethyl-(dimethylprophilamine) carbodiimide (EDAC).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-03-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1512
10.14499/indonesianjpharm30iss1pp52
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 52
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1512/893
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/657
2020-01-27T02:53:58Z
3:ART
EFFECT OF BENZALDEHYDE EXCESS IN THE SYNTHESIS OF LR-2 AND CYTOTOXIC ACTIVITY OF LR-2 AGAINTS HeLa CELL
Ritmaleni, Ritmaleni; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip, Utara Yogyakarta 55281, Indonesia
Arifin, Muhammad Fajar; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip, Utara Yogyakarta 55281, Indonesia
Laksmiani, Ni Putu Linda; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Udayana, Bali 80221, Indonesia.
., Rumiyati; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip, Utara Yogyakarta 55281, Indonesia
., Sismindari; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip, Utara Yogyakarta 55281, Indonesia
LR-2(4-phenyl-3,4-dihydro-indeno[2’,1’]pyramidine-2(1H)- thione; Leni Ritmaleni 2), which designed and assumed to have biologically activity as anticancer, has been successfully synthesized by using the Biginelli reaction. This research was aimed to investigate the effect of benzaldehyde excess in the synthesis of LR-2 and to evaluate the cytotoxic activity of LR-2against HeLa cancer cell lines. The synthesis was done by reacting benzaldehyde, 2-indanone and together with thiourea at one time as said as one pot reaction synthetic methodology and the reaction was acid catalysed. The mole equivalent of benzaldehyde was in excess compare to others. The effect of benzaldehyde in excess is the higher the mole of benzaldehyde, the lower the yield of LR-2. The cytotoxicity of LR-2 was done by using MTT method and the LC50 was 268.15 μM.Key words : LR-2, benzaldehyde, cytotoxic, HeLa
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/657
10.14499/indonesianjpharm23iss1pp9-17
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 9-17
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/657/523
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1115
2017-08-30T06:55:29Z
3:ART
Resorcinol Compounds Isolated form the Bark of Myristica fatua Houtt.
Megawati, Megawati; Research center of chemistry-LIPI
Darmawan, ahmad; Research center of chemistry-LIPI
Myristica fatua Houtt., resorcinol, malabaricone B, malabaricone C, cytotoxic
Myristica fatua Houtt. is one of the endemic plants growth in Wallace region. Isolation of the secondary metabolite compounds from the ethyl acetate fraction of the bark of Myristica fatua Houtt. has been done using some chromatography techniques afforded two resorcinol compounds, malabaricone C (1), and malabaricone B (2). The structures of 1-2 were determined using spectroscopic techniques, including mass spectrometry, 1D-NMR and 2D-NMR. Both compounds showed in vitro cytotoxic activity against the breast carcinoma cancer cell lines MCF-7 using alamar blue assay method, with IC50 of 2.38 and 0.71 μg/mL, respectively.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-06-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1115
10.14499/indonesianjpharm28iss2pp82
Indonesian Journal of Pharmacy; Vol 28 No 2, 2017; 82
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1115/815
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/946
2017-08-03T01:55:59Z
3:ART
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/678
2020-01-27T02:53:16Z
3:ART
Satyrium nepalense: A RARE MEDICINAL ORCHID OF WESTERN HIMALAYA (INDIA); PHYTOCHEMICAL SCREENING, ANTIMICROBIAL EVALUATION AND CONSERVATION STUDIES
Mishra, Abhay Prakash; Department of Pharmaceutical Chemistry, H.N.B. Garhwal (A Central) University Srinagar, Uttarakhand, INDIA. 246174
Saklani, Sarla; Department of Pharmaceutical Chemistry, H.N.B. Garhwal (A Central) University Srinagar, Uttarakhand, INDIA. 246174
Satyrium nepalense is an endangered medicinal herb found at the higher altitude of 2400-5000m. Local inhabitant used tubers of Satyrium nepalense as an energetic tonic and as an important medicine to cure different type of fever in traditional health care system of Uttarakhand (INDIA). Present study was carried out to evaluate the phytochemical, antibacterial screening against four (Streptococcus mutans, Pseudomonas aeruginosa, Staphylococcus aureus and Klebsiella pneumoniae) microorganisms in methanolic extracts and habitat studies for conservation of S. nepalense. Tubers of Satyrium nepalense were extracted separately with methanol by hot extraction process using soxhlet apparatus. The extracts were subjected to Lyophilization to get dry extract and preserved in aseptic condition. The different group reagents used for phytochemical screening as dragendorffs’s for alkaloid, molisch’s test for carbohydrates, shinoda test for flavonoids etc. Antibacterial study was carried out by disc diffusion method. The highest zone of inhibition was recorded as 15.0±0.00 mm against Klebsiella pneumonia and 15.0±0.82 mm against Staphylococcus aureus. Phytochemical screening shows the presence of alkaloids, carbohydrates/glycosides, flavonoids and unsaturated sterols/triterpenes in Satyrium nepalense. The result indicates that methanolic extract of Satyrium nepalense shows potent antibacterial activity against all four bacterial strains.Key words: Uttarakhand, Satyrium nepalense, medicinal orchid, antibacterial, phytochemicals
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/678
10.14499/indonesianjpharm23iss3pp162-170
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 162-170
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/678/544
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1153
2017-08-30T06:51:26Z
3:ART
INHIBITORY ACTIVITY OF ENDOPHYTIC FUNGI ISOLATED FROM SUKABUMI TURMERIC PLANT (Curcuma longa L.) AGAINST MCF-7 CELL LINE
Ningrum, Ratih Asmana; Research Center for Biotechnology
Indonesian Institute of Sciences
Bustanussalam, Bustanussalam; Research Center for Biotechnology
Indonesian Institute of Sciences
Wisnuwardhani, Popi Hadi; Research Center for Biotechnology
Indonesian Institute of Sciences
Herawati, Neng; Research Center for Biotechnology
Indonesian Institute of Sciences
Santoso, Adi; Research Center for Biotechnology
Indonesian Institute of Sciences
Simanjuntak, Partomuan; Research Center for Biotechnology
Indonesian Institute of Sciences
MCF-7; inhibitory activity; endophytic fungi; turmeric plant
Kunyit or turmeric plant (Curcuma longa L.) is a native Southeast Asia plant that has been widely used as herbal medicine. In our previous research we isolated and screened 44 of endophytic fungi of turmeric plant from Sukabumi and Cibinong to determine their antioxidant activity. There were 4 samples isolated from Sukabumi with antioxidant activity for more than 70% at concentration of 100ppm, K.Cl.Sb.R9 (93%), K.Cl.Sb.A11 (81%), K.Cl.Sb.B1 (79%) and K.Cl.Sb.R11 (71%). This research aimed to determine inhibitory activity of the endophytic fungi against estrogen positive MCF-7 breast cancer cell line. To obtain the filtrate, the broth cultures were filtered and the extracellular fraction was extracted with ethyl acetate. The inhibitory activity was determined by using MTT assay. The result showed that the ability of endophytic fungi to inhibit MCF-7 growth was dose dependently. IC50 of endophytic fungi K.Cl.Sb.R9 was 579±38 ppm, K.Cl.Sb.R11 was 542±21ppm, K.Cl.Sb.B1 was 446±15ppm and K.Cl.Sb.A11 was 520±28ppm. Fluorescent double staining based method using calcein AM and ethidium bromide was performed to confirm the inhibitory activity. At 500ppm of filtrate concentration with 24h of cell treatment, treated cell lines showed fewer viable cells compared to untreated cell lines. The four isolates of endophytic fungi were able to inhibit proliferation of human breast cancer MCF-7 cell line.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-03-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1153
10.14499/indonesianjpharm28iss1pp19
Indonesian Journal of Pharmacy; Vol 28 No 1, 2017; 19
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1153/800
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1545
2019-10-28T01:20:02Z
3:ART
Effect of 7-Hydroxy-2-(4- Hydroxy -3-Methoxyphenyl)-Chroman-4-one On Level of Mangan-Superoxide Dismutase (Mn-Sod) and Superoxide Dismutase 2 (Sod2) Gene Expression in Hyperlipidemia Rats
Ayunda, Rahmah Dara; Magister of Biomedical Science Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada
Prasetyastuti, Prasetyastuti; Departement of Biochemistry Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada
Hastuti, Pramudji; Department of Biochemistry Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada, Skip Utara 55281 Yogyakarta
Hyperlipidemia, flavonoids, Mn-SOD, SOD2 gene
Hyperlipidemia is a lipid metabolism disorder characterized by an increase in serum lipid levels. Hyperlipidemia is a major risk factor for many metabolic syndrome diseases because it triggers oxidative stress. Oxidative stress can be reduced by endogenous antioxidant enzymes triggered by exogenous antioxidant compounds, such as 7-OH-2- (4-OH-3-methoxyphenyl)-chroman-4-one isolated from the seeds of Swietenia macrophylla King. The aims of this study were to investigate the effects of 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one compounds on cholesterol level, LDL level, Mn-SOD levels and SOD2 gene expression of hyperlipidemic rats. Thirty rats (Rattus norvegicus ) were divided into 6 groups, normal group (N), hyperlipidemia group (HL), hyperlipidemia group with simvastatin (P), hyperlipidemic group with 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one with dose 10 (F10), 30 (F30) and 90 (F90) mg/200g body weight (BW). Cholesterol and LDL were analyzed with CHOD-PAP method, Mn-SOD level was analyzed by ELISA method and SOD2 gene expression was analyzed by qPCR method. The decrease in cholesterol and LDL levels were most prevalent in group F90 with dose 90 mg/200g BW of 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one, with average difference each of them was 172.43 mg/dL and 36.12 mg/dL. The rats fed on high-cholesterol diet exhibited a significant elevation in Mn-SOD levels (p<0.05) compared to normal group. The treated animals with 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one has the level of Mn-SOD is significantly lower (p<0.05) compared with hyperlipidemic group. Expression of SOD2 in group F90 has value close to normal group (p> 0.05). 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one with dose of 90 mg/200g BW improved cholesterol levels, LDL levels, Mn-SOD levels and SOD2 gene expression in hyperlipidemic rats.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-08-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1545
10.14499/indonesianjpharm30iss3pp180
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 180
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1545/915
Copyright (c) 2019 Indonesian Journal of Pharmacy
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/441
2020-02-19T02:37:07Z
3:ART
PREFORMULATION OF DICLOFENAC SODIUM SUSTAINED RELEASE BY ION EXCHANGER RESINE
Zulkarnain, A. Karim; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
Yuwono, Tedjo; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
., Sumarno; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
The aim of the study was to find out a formula of granules giving a constant release of drug and also to search for the influence of pH on the drug release from the formula. One type of drug-resin complexes was used in this study ion exchanger the complex of diclofenac sodium and dowex. After 12 hours mixing the drug-resin complex was dried at 40 oC in an oven for 3 days. Afterwards, they were granulated and shieved. The particle size used were 20-40 mesh. The granules were tested for the dissolution of diclofenac sodium from the granules. The dissolution experiments were performed in a modified model of USP XX and the dissolution media used were phosphate buffer with the pHs of 5,8; 6,8 and 7.6 and the temperature was maintained at 37 oC. The amount of diug released in to the medium was assayed spectrophotometrically. The extent of dissolution was expressed as dissolution efficiency in 8 hours (DE8, % ). The results showed that the extent of dissolution of dtclofenac sodium from the granules complexed with the resin were low. The correlation between the amount of drug release from the complex and time was linier with the highest coefficient correlation having the ratio of l:5. The higher the pH values of the media, the higher the drug release from granules was. The drug release from drug-resin complex was well controlled which may be used for making a sustained release dosage form.Key Word : sustained release, diclofenac sodium, ion exchanger resin, granules
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/441
10.14499/indonesianjpharm0iss0pp20-27
Indonesian Journal of Pharmacy; Vol 12 No 1, 2001; 20-27
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/441/320
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1101
2018-10-23T02:19:20Z
3:ART
Formulation and Evaluation of Glipizide Tablets Utilizing Hibiscus Rosasinensis Leaves Mucilage
Bahadur, Sanjib; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Roy, Amit; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Baghel, Pragya; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Choudhury, Ananta; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Saha, Suman; Columbia Institute of Pharmacy, Village Tekari, Near Vidhan Sabha, Raipur, Chhattisgarh, 493111
Chanda, Ranabir; Bengal School of Technology, Sugandha, Delhi Road, Hooghly, West Bengal, 712102
This research work aims to develop glipizide tablets using Hibiscus rosasinensis leaves mucilage. The mucilage was extracted by using double distilled water and precipitated with ethanol. The precipitated mucilage was dried and grounded into powder. The tablet utilizing the mucilage as excipient was prepared by wet granulation method. The tablets were subjected to various tests. The evaluatory parameters of tablets were found to be within the limits as per United States Pharmacopoeia NF 24/19. FTIR spectrum reveals that there is no incompatibility between the ingredients used. Diabetes was induced in wistar albino rats using streptozotocin and effect of formulation on blood glucose level was determined. It was observed that the formulation could not sustain the release of glipizide. However, the glipizide release was retarded as the amount of mucilage was increased. It was observed that on the completion of antidiabetic study, the formulation could bring the blood glucose level to normal in group rats. However, the blood glucose level was still elevated in group administered with pure gliplizide. It can be concluded that HRM can be used to formulate glipizide tablets. The formulations with HRM shows better hypoglycemic activity and this may be attributed to antidiabetic activity of Hibiscus rosasinensis.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-03-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1101
10.14499/indonesianjpharm29iss1pp23
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 23
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1101/860
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/948
2017-09-11T04:55:14Z
3:ART
ANALYSIS OF FACTORS AFFECTING PATIENT SATISFACTION IN JKN SYSTEM: PATIENT CHARACTERISTICS, JKN SERVICES, AND PHARMACY SERVICES
Satibi, Satibi; Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara 55281
National Health Insurance (JKN) is a health insurance system by using mechanisms of social health insurance held by BPJS. Satisfaction of participants will affect the sustainability of the system JKN. Factors affecting patient satisfaction are patient characteristics, JKN services, and pharmacy services. This study aimed to examine the influence of patient characteristics, JKN services, and pharmacy services with patient satisfaction in the primary health facilities. This research is analytic with cross sectional survey design. Data taken quantitatively by using questionnaires and equipped with qualitative data from interviews to deepen the findings in the field. The sampling technique used is random sampling for primary health facilities with a total sample of 55 health facilities and purposive sampling for a sample of patients with a total sample of 300 respondents. Data were analyzed using Chi-Square to examine relationship between patient characteristics with patient satisfaction and linear regression to examine the relationship between services in JKN system with patient satisfaction. Characteristics of patients who have a significant relationship to the patient satisfaction is education (p=0.020), employment (p = 0.001), income (p = 0.000), and membership status (p = 0.005).There is a significant relationship between services in JKN system includes JKN sevices with patient satisfaction (p = 0.000) and pharmacy service in the era of JKN with patient satisfaction (p = 0.000). The conclusion of this study is the patient characteristics include education, employment, income and membership status, and service in JKN system includes JKN services and pharmacy services in the era of JKN has a significant relationship with the satisfaction of patients at primary health facilities.Keywords: patient characteristics, JKN services, pharmacy services, patient satisfaction
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2015-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/948
10.14499/indonesianjpharm26iss4pp233
Indonesian Journal of Pharmacy; Vol 26 No 4, 2015; 233
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/948/759
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/948/123
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1184
2018-10-23T02:19:20Z
3:ART
The Effect of Dual Antiplatelet Post Percutaneous Coronary Intervention On Aggregation of Platelet In Myocardial Infarction Patients With Diabetes Mellitus and Non Diabetes Mellitus
Rahmawati, Yessi Asli; Airlangga University
Yogiarto, Mohammad; Dr.Soetomo Teaching Hospital Surabaya
Zulkarnaen, Bambang Subakti; Airlangga University
To analyze the differences in the effect of dual antiplatelet post PCI on the percentage of aggregation in myocardial infarction patients with DM and non DM. Percentage of aggregation were analyzed using light transmission aggregometry (LTA) before loading dose, after PCI, and after maintenance dose of dual antiplatelet (aspirin 100mg and clopidogrel 75mg). Total 22 patients were participated in this study divided into 10 and 12 patients in diabetic and non diabetic group. Percentage of aggregation after taking dual antiplatelet maintenace dose decrease significantly in both group (p=0.006 in diabetic group and p=0.002 in non diabetic group). Mean reduction of percentage of aggregation in diabetic group (3.30±2.91%) is less than non diabetic group (6.83±5.97%). Statistical analysis shows that the mean reduction of percentage of aggregation between two groups were not significantly different (p>0.05). Mean percentage of aggregation after dual antiplatelet maintenance dose was higher in diabetic group and mean reduction of percentage of aggregation was higher in non diabetic group, although statistically in both group it is not significantly different.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-01-17
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1184
10.14499/indonesianjpharm29iss1pp44
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 44
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1184/862
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1529
2021-01-06T13:13:53Z
3:ART
Inhibition of Cell Cycle and Induction of Apoptosis y Ethanol Leaves Extract of Chrysanthemum cinerariifolium (Trev.) In T47D Breast Cancer Cells
Mutiah, Roihatul; Department of Pharmacy, Faculty of Medicine and Health Science, Maulana Malik Ibrahim Islamic State University of Malang
Inayatin, Alfiyah Laily; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
Annisa, Rahmi; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
Indrawijaya, Yen Yen Ari; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
Listiyana, Anik; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
C. cinerariifolium, apoptosis, cell cycle, T47D cells
Chrysanthemum cinerariifolium (C.cinerariifolium) is a plant of the Asteraceae family, which has been applied by the community as an ornamental plant and traditional medicine. In this study, the effect of C. cinerariifolium leaves extract on inhibition of cell cycle and induction of apoptosis in T47D breast cancer cells was tested and compared to the standard chemotherapy agent. The citotoxic activity of C. cinerariifolium leaves extract against T47D cancer cells and Vero normal cells was tested by MTT method. Profile of apoptosis and cell cycle were observed by flow cytometry method. Based on chemical compounds profil which is tested used TLC showed that C.cinerariifolium leaves extracts contained flavonoid and terpenoid chemical compounds. The result of cytotoxic test showed that leaves extract of C. cinerariifolium was able to inhibit the growth of T47D cancer cell at IC50 418.8μg/mL. Doxorubicin, extracted from Streptomyces peucetius used as treatment in several cancers including breast cancer. Doxorubicin could inhibit the growth of T47D cancer cells in 115.1μg/mL. The results of cell cycle analysis showed that the C. cinerariifolium leaves extract inhibited cell cycle in G0-G1 and S phase, whereas doxorubicin was able to inhibit cell cycle in G0-G1 phase but experienced cell accumulation in G2-M phase. The percentage of apoptosis in cycle was showed in M1 (sub G1) and M5 (multinuclear) phase which treatment of C. cinerariifolium leaves extract was higher than doxorubicin. Therefore, C. cinerariifolium leaves extract has potential activity as anticancer agent causes inhibition of cell cycle and induction apoptosis.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
This work supported by The Directorate General of Islamic Higher Education (DIKTIS) of Interdisciplinary Basic Research Grant numbers 3209/Un.3/HK.00.5/05/2018.
2020-04-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1529
10.14499/indonesianjpharm31iss1pp1
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 1
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1529/937
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/590
2017-09-20T03:28:39Z
3:ART
Synthesis and cytotoxicity test of LR-2 on breast cancer cell line T47D
Ritmaleni, Ritmaleni; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada. Yogyakarta, Indonesia
Anitasari, Dina; Faculty of Pharmacy, Universitas Gadjah Mada. Yogyakarta, Indonesia
Susanti, Sinta; Faculty of Pharmacy, Universitas Gadjah Mada. Yogyakarta, Indonesia
., Sismindari; Faculty of Pharmacy, Universitas Gadjah Mada. Yogyakarta, Indonesia
LR-2 is a compound analog of monastrol, an anticancer agent, which has been succesfully synthesised through the application of Biginelli reaction and one of LR compound series that synthesised in Faculty of Farmacy, Universitas Gadjah Mada. This reserach was aimed to synthesized the LR-2 compound and to investigate the cytotoxicity of LR-2 on breast cancer cell line. The reaction was carried out by using the one pot reaction method. In this reaction benzaldehyde, 2-indanone and thiourea were reacted together for 6hours in acid catalyst. The cytotoxicity test was carried by using breast cancer cell line the MTT assay method and the LC50 was ditermined by using the probit analysis with Miller and Tainter method. The product was isolated by using preparative TLC in 54 % yield. The structure was elucidated by spectroscopymethods (UV-Vis, H-NMR, IR and GC-MS).The LC50 is 159 µM.Keywords : LR-2, benzaldehyde, 2-indanone, thiourea, T47D
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2011-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/590
10.14499/indonesianjpharm0iss0pp21-32
Indonesian Journal of Pharmacy; Vol 22 No 1, 2011; 21-32
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/590/465
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1388
2019-10-03T03:16:50Z
3:ART
Screening of Antibacterial and Anticancer Activity of Soft Corals from Togean Islands, Indonesia
Zubair, Muhammad Sulaiman; Department of Pharmacy, Faculty of Sciences, Tadulako University, Kampus Bumi Tadulako, 94118, Palu, Indonesia
Lallo, Subehan; Faculty of Pharmacy, Hasanuddin University, 90245, Makassar, Indonesia
Rusmiyanti, Rusmiyanti; Department of Pharmacy, Faculty of Sciences, Tadulako University, Kampus Bumi Tadulako, 94118, Palu, Indonesia
Nugrahani, Arsa Wahyu; Department of Pharmacy, Faculty of Sciences, Tadulako University, Kampus Bumi Tadulako, 94118, Palu, Indonesia;
Jantan, Ibrahim; School of Pharmacy, Taylor’s University, Lakeside Campus, 1 Jalan Taylors, 47500 Subang Jaya, Selangor, Malaysia
Softcorals, Togean Islands, anti-cancer, antibacterial
Soft corals (Octocorallia, Alcyonaceae) have been reported to possess diverse biological activities and unique structural chemistry. This study aims to screen the potential antibacterial and anticancer activity of some soft corals collected from Togean Islands, Central Sulawesi, Indonesia. They were Lobophytum sp, Sarcophyton sp, Sinularia sp 1, and Sinularia sp 2. All dried coral materials were extracted for 3 x 24 h by maceration method using methanol and then evaporated by rotary evaporator to obtain viscous extracts. The determination of antibacterial activity had been performed by well agar diffusion method against Staphylococcus aureus and Escherichia coli. Meanwhile, the cytotoxic activity was performed by MTT method, followed by apoptosis annexin V-FTIC assay agains. Identification for the presence of terpenoids was performed by vacuum p-anisaldehyde-sulphuric acid spraying reagent on thin layer chromatography (TLC). Sinularia sp2 extract have strongly inhibited S. aureus and E.coli with the diameter of inhibition range from 12.76mm and 17.86mm, respectively. Moreover, Sinularia sp2 extract possessed also cytotoxic activity against human breast adenocarcinoma (MCF-7) and colorectal carcinoma (HCT-116) with the IC50 of 46.807 and 47.186 μg/mL, respectively. Extract Sinularia sp 1 was found to have strongest cytotoxicity on human colon colorectal carcinoma (HCT-116) with the IC50 of < 1.505 μg/mL. Annexin V-FTIC assay clearly exhibited that the apoptosis mechanism is proposed by the extracts of Sinularia sp1 and Sinularia sp 2. Terpenoids were identified on both extracts suggesting for further purification and isolation for the bioactive terpenoid compounds.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-11-21
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1388
10.14499/indonesianjpharm29iss4pp173
Indonesian Journal of Pharmacy; Vol 29 No 4, 2018; 173
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1388/880
Copyright (c) 2018 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1988
2021-01-07T01:00:39Z
3:ART
In vitro Comparative Study for Anti-proliferative Activity of Some Plant Extracts, Fam. Apiaceae, on HeLa Cell Line
Gomaa, Sara E.; Medicinal and Aromatic Plants Research Dept., Horticulture Research Institute (HRI), Egypt. 9 ALgamaa Street, Giza, Egypt
Friedersdorf, Matthew; Molecular Genetics and Microbiology Dept., Duke University Medical Center, NC, USA
El Enshasy, Hesham Ali; Director,
Institute of Bioproduct Development (IBD), Universit Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Abou-Donia, MB; Pharmacology and Cancer Biology Dept., Duke University School of Medicine, NC, USA.
Apiaceae - Anti-proliferative - Extracts - HeLa Cell Line
In this research, the biological activities of five plant extracts from family Apiaceae; Italian Parsley (Petroselinum neapolitanum), Fennel (Foeniculum vulgare), Celery (Apium graveolens), Cilantro (Coriandrum sativum) and Dill (Anethum graveolens), were studied. Antiproliferative effect of eleven ethanol crude extracts was tested in Human Cervical (Hela) cancer cells. Results clearly demonstrated that all plant extracts showed high significant difference when compared to the negative control (DMSO).Parsley leaves extract, cilantro leaves extract and cilantro stems extract showed no significant difference with the positive control (Actinomycin D). As for, fennel bulb extracts, fennel stalks extracts, celery stems gave better results than the positive control with no significant difference through the 24, 48 and 72 h treatment. There were no significant difference between Fennel extracts and the positive control, which showed high effect on the cancer cells survival. There were no significant difference between both extracts of Cilantro leaves and stems through each time but the best result was after 72 h of treatments. Regarding Dill leaves and stems, cell numbers recorded no significant difference between the both on time dependent manner. Further investigation for ethanolic extracts of parsley leaves, fennel bulb, fennel stalks, celery stems, cilantro leaves and cilantro stems which showed better results than using the commercial drug Actinomycin D (25ml/ml) for 24 h treatment or less depending on concentrations manner. Also, further investigation on different types of cancer cell lines to avoid the toxic effect of chemotherapy.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Jack Kenee, Duke University, USA
2020-08-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1988
10.14499/indonesianjpharm31iss2pp108
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 108
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1988/946
Copyright (c) 2020 Indonesian Journal of Pharmacy
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/675
2020-01-27T02:53:16Z
3:ART
ISOLATION AND IDENTIFICATION OF FLAVONOIDS FROM Sesamum indicum
Sharma, Priyanka; Laboratory of Bioactive Compounds and
Algal Biotechnology Department of Botany,University of Rajasthan, Jaipur- India.
302004
Sarin, Renu; Laboratory of Bioactive Compounds and
Algal Biotechnology Department of Botany,University of Rajasthan, Jaipur- India.
302004
Natural substances have long served as sources of therapeutic drugs. Many substances have been derived from traditional medicines. The plants are rich in secondary metabolites. The medicinal properties of these plants have been attributed to the biochemicals present in the plant materials. In addition to their role in human and animal nutrition, knowledge of micronutrients and phytochemical composition is fundamental to the understanding of modes and mechanisms of action of medicinal plants in general. In the present investigation, quercetin and kaempferol have been isolated and identified from stem, leaves and unorganized cultures of Sesamum indicum and maintained by frequent subculturings on Murashige and Skoog’s medium (1962) supplemented with NAA+BAP(5.0+0.5mg/L). The study showed that maximum content of quercetin and kaempferol was observed in 6 weeks old calli and minimum in stem of S. indicum. The structure of the isolated compound was established on the basis of physical, chemical test and spectroscopic evidences.Key words: Flavonoids, quercetin, kaempferol, Sesamum indicum
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/675
10.14499/indonesianjpharm23iss3pp135-140
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 135-140
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/675/541
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1062
2017-09-11T06:05:22Z
3:ART
AN LC- MS/MS METHOD FOR THE DETERMINATION OF OMEPRAZOLE ON PROTON PUMP INHIBITOR IN HUMAN PLASMA
Sudha, T.; Dept Pharma. Analysis, The Erode College of Pharmacy, Erode TamilNadu, India. 638112
Reddy, Kalan Kumar
Hemalatha, P. V.
Ravikumar, V. R.
A sensitive and selective liquid chromatographic method coupled with tandem mass spectroscopy (LC-MS/MS) was developed for the quantification of omeprazole in human plasma. Lansoprazole was used as internal standard with plasma samples, extracted using 10mM ammonium acetate. A centrifuged upper layer was then evaporated and reconstituted with Acetonitrile: mobile phase buffer 70:30%v/v. The reconstructed samples were injected into a C18 column purospher star 5µ. The mobile phase was composed of ACN: mobile phase buffer (5mm ammonium bicarbonate buffer) in the ratio of 70:30%v/v with flow rate 1.0mL/min. The mass spectrometer was operated using positive ion mode and turbo electro spray ionisation. Nitrogen was used as the nebulizer, curtain, collision and auxiliary gases. Using MS/MS with multiple reactions monitoring (MRM) mode, omeprazole was detected without severe interferences from plasma matrix. Detection of omeprazole in human plasma was accurate and precision. This method has been successfully applied to the study of omeprazole in human specimensKeywords: Proton pump inhibitor, omeprazole, lansoprazole, LC-MS/MS, liquid liquid extraction
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-03-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1062
10.14499/indonesianjpharm27iss2pp80
Indonesian Journal of Pharmacy; Vol 27 No 2, 2016; 80
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1062/773
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1467
2019-10-28T01:20:02Z
3:ART
Anticancer Molecules from Catharanthus roseus
Taher, Zarani M; Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Agouillal, Farid; 1. Research Unit on Analysis and Technological Development in Environment, URADTE, Centre de Recherche Scientifique et Technique en Analyses Physico-Chimiques, CRAPC, B.P. 384, RP 42004 Zone Industrielle Bou-Ismail, Tipaza, Algeria.
2. Laboratory of Reaction Engineering, LGR, Faculty of Mechanical Engineering and Process Engineering, Houari Boumediene University of Science and Technology, USTHB, PB 32 El Alia, 16075, Bab Ezzouar, Algiers, Algeria.
R, Lim J.; School of Chemical Engineering and Energy, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Marof, Aina Q; Department of Chemistry, Mathematics and Science Faculty, Universitas Negeri Jakarta, Jakarta, Indonesia
Dailin, Daniel Joe; 1. Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
2. School of Chemical Engineering and Energy, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Nurjayadi, Muktiningsih; Department of Chemistry, Mathematics and Science Faculty, Universitas Negeri Jakarta, Jakarta, Indonesia
Razif, Ezzaty NM; Faculty of Applied Sciences, Universiti Teknologi Mara Negeri Sembilan, Kampus Kuala Pilah, Pekan Parit Tinggi 72000, Kuala Pilah Negeri Sembilan, Malaysia
Gomaa, Sara E; Horticulture Research Institute, Agriculture Research Center, Egypt.
El Enshasy, Hesham Ali; Director
Institute of Bioproduct Development (IBD)
Universiti Teknologi Malaysia (UTM)
Skudai, Johor Bahru, Malaysia
C. roseus; Anticancer; Extraction; Phytochemical; Tissue culture
Catharanthus roseus is an important medicinal plant found in various parts of the world and the bioactive compound has been extracted and used as anti-cancer agent to treat the cancer over decades. However, the extraction of bioactive compound also results in the generation of large quantities of pollution with wasted solvents. Toxic pollution occurs when synthetic chemicals are discharged or natural chemicals accumulate to toxic levels in the environment, causing reductions in wildlife numbers, degrading ecosystem functions and threatening human health. This review covers the extraction and phytochemical obtained leading to chemical compounds related to anti-cancer property of C. roseus. Additionally, recent advances of using biological cell cultures were also addressed. Thus, this work can be used for further investigation of C. roseus to be undertaken in future for its anti-cancer property further development and efficient production in drug industry
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
MOE and UTM-RMC (Malaysia) through HICOE grant no. R.J130000.7846.4J262.
2019-07-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1467
10.14499/indonesianjpharm30iss3pp147
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 147
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1467/910
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/373
2017-09-20T03:27:45Z
3:ART
Synthesis of 4-phenyl-3,4-tetrahydro-indeno [2,1]-pyrimidin-2-one (LR-1)
Ritmaleni, Ritmaleni; Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta
Nurcahyani, Wahyu; Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta
The synthetic compound 4-phenyl-3,4-tetrahydro-indeno[2,1]- pyrimidin-2-one 20a (LR-1) was synthesised using Biginelli reaction method. The reaction involved benzaldehyde 6, 2-indanone 2 and urea 7 in acid condition. This condensation reaction yielded 15 % of the product 20, at 133,4-135,0oC of melting point and 0.15 (Et2O : CHCl3 = 1 : 3) of Rf value.Keywords : benzaldehyde, indenone-2, urea
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2006-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/373
10.14499/indonesianjpharm0iss0pp149-155
Indonesian Journal of Pharmacy; Vol 17 No 3, 2006; 149-155
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/373/254
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1104
2018-10-23T02:18:57Z
3:ART
Population-Based Approach to Analyze Sparse Sampling Data in Biopharmaceutics and Pharmacokinetics using Monolix and NONMEM
Nugroho, Akhmad Kharis; Department of Pharmaceutics Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta Indonesia
Hakim, Arief Rahman; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada
Hakim, Lukman; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada
model, population, sparse sampling data, Monolix, NONMEM
Although it has been developed since 1972, the implementation of a population-based modeling approach in Indonesia, particularly to analyze biopharmaceutics and pharmacokinetics data is still very limited. This study was aimed to evaluate the performance of Monolix and NONMEM, two of the popular software packages in a population-based modeling approach, to analyze the limited data (sparse sampling data) of the time profiles of the simulated plasma drug concentration of a theoretical compound. and NONMEM were used to model the limited data (40 data points) as a results of the random selection from the 180 point data of simulated plasma drug concentration (Cp) on 20 subjects at 0.25; 0.5; 0.75; 1; 1.5; 3; 6; 12 and 18 hours after per-oral administration of a 100mg of a theoretical compound. Population values of the absorption rate constant (Ka), the elimination rate constant (Kel) and volume of distribution (Vd) were compared to the average Ka, Kel and Vd obtained by the conventional method (two stage approach) using PKSolver on the Cp data of all subjects. The calculation system of a nonlinear mixed effect model in Monolix and NONMEM, successfully describes the sparse data, based on the visual evaluation of the goodness of fit. Comparison of parameter estimates of population values in Monolix and NONMEM are in the range of 94 to 108% of the real values of the rich data analysed by PKSolver. A population-based modeling can adequately analyze limited or sparse data, demonstrating its capability as an important tool in clinical studies, involving patients.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-12-06
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1104
10.14499/indonesianjpharm28iss4pp205
Indonesian Journal of Pharmacy; Vol 28 No 4, 2017; 205
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1104/853
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/689
2020-01-27T02:53:31Z
3:ART
PHYTOCHEMICAL STUDIES OF Acacia catechu
Baluja, Shipra; Department of Chemistry, Saurashtra University, Rajkot-360005 (Gujarat), INDIA
Chanda, Sumitra; Department of Biosciences, Saurashtra University, Rajkot-360005 (Gujarat), INDIA.
Solanki, Asif; Department of Biosciences, Saurashtra University, Rajkot-360005 (Gujarat), INDIA.
Kachhadia, Nikunj; Department of Biosciences, Saurashtra University, Rajkot-360005 (Gujarat), INDIA.
Acacia catechu Willd. belongs to the family Mimosaceae. It is well known for the powder of wood known as Kathha” in India, which has wide applications. In the present work, the dry wood powder is extracted in various solvents such as 1,4-dioxane, methanol, dimethyl formamide (DMF), acetone, ethanol and distilled water. The phytochemical and physicochemical properties of these various solvent extracts were studied. The phyto constituents present in the different extracts were saponins, tannins, sugars, terpenes, catechu-tannic acid. Alkaloids were present in organic solvents only. The antibacterial activity of all the extracts in DMSO was studied against five bacterial strains but none of the extracts inhibit any of the studied bacterial strains.Key words: Acacia catechu, 1,4-dioxane, methanol, dimethyl formamide (DMF), acetone, ethanol, distilled water, phytochemical analysis, physicochemical analysis.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/689
10.14499/indonesianjpharm23iss4pp238-247
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 238-247
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/689/555
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1157
2017-09-11T06:07:57Z
3:ART
PREPARATION AND CHARACTERIZATION OF CO-CRYSTALS OF DIACEREIN
Thenge, Raju Rameshrao; Department of Industrial Pharmacy, IBSS College of Pharmacy
Patond, V B; Department of Industrial Pharmacy, IBSS College of Pharmacy
Ajmire, P V; Department of Industrial Pharmacy, IBSS College of Pharmacy
Barde, L N; Department of Industrial Pharmacy, IBSS College of Pharmacy
Mahajan, N M; Department of Pharmaceutics, Dadasaheb Balpande College of Pharmacy
Tekade, N P; SGSPS Institute of Pharmacy
Co-crystals; Diacerein; physicochemical properties; FT-IR
Diacerein, anti-inflammatory drug used in the treatment of osteoarthritis. Being a BCS class II drug, it has poor solubility, dissolution rate and other physicochemical properties. Thus the aim of present study was to prepare co-crystals of diacerein to improve solubility, dissolution rate. The diacerein co-crystals were prepared using urea and tartaric acid as conformer by Solvent drop grinding method. The diacerein co-crystals were characterized by scanning electron microscopy (SEM), FT-IR spectroscopy (FT-IR), Differential scanning calorimetry (DSC) and X-ray diffractometry (XRD). The co-crystals were evaluated for solubility, dissolution rate and other physicochemical properties and compared with commercial diacern sample. The co-crystals exhibit the difference in the size and shape of crystals. The FT-IR spectra of diacerein co-crystals showed slightly different in the characteristic peaks compared to commercial diacerein sample. DSC data indicate the decrease in the melting endotherm of co-crystals compare to diacerein. The co-crystals with urea showed increase and intense peak and co-crystals with tartaric acid showed decreased number of peaks compared to commercial diacerein. The co-crystals of diacerein formulated in to the Tablet and evaluated for tablet properties. The tablet formulation showed improved tablet characteristics as well as dissolution rate compared to commercial diacerein.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-03-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1157
10.14499/indonesianjpharm28iss1pp34
Indonesian Journal of Pharmacy; Vol 28 No 1, 2017; 34
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1157/802
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1470
2022-10-26T01:05:53Z
3:ART
Quality of Life of Patient with Hypertension in Primary Health Care in Bandar Lampung
Khoirunnisa, Sudewi Mukaromah; Institut Teknologi Sumatera
Akhmad, Atika Dalili; Institut Teknologi Sumatera
Hypertension; Quality of Life; SF-36
The high prevalence of hypertension in Indonesia, encourages studies related to how the quality of life of patients with hypertension. The purpose of this study was to measure quality of life, identify and explain factors related to the quality of life of patients with hypertension.The study design was descriptive correlation using a cross sectional study approach. The study subjects were all outpatient hypertensive patients several health centers in Bandar Lampung. The instrument used in this study was the Indonesian SF-36. The data collected included the patient's demographic characteristics including gender, age, education, occupation, and marital status, and the fields related to the health history of the study subjects included the duration of hypertension, complications, and the number of antihypertensive drugs consumed. Data were analyzed using bivariate analysis to see the relationship between two variables with the level of significance used was 5% (α = 0.05) with the value of the confidence interval set was 95%. Multivariate analysis was conducted to study the relationship of several independent variables with one or several dependent variables.The results of univariate analysis showed that age, marital status, duration of illness, complications, and the number of drugs consumed had an effect on the quality of life of hypertensive patients (p <0.05). The results of multivariate analysis showed that the factors of age, marital status, and duration of hypertension are factors that influence the physical domain, while the factors of gender, marital status, duration of hypertension, complications, and the number of drugs are influential factors in the mental domain mental.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
The Ministries of Research, Technology and Higher Education of Indonesia
2020-01-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1470
10.14499/indonesianjpharm30iss4pp309
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 309
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1470/927
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1470/200
Copyright (c) 2019 Indonesian Journal of Pharmacy
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/513
2018-09-05T01:49:55Z
3:ART
SYNTHESIS AND ANTIMICROBIAL ACTIVITY EVALUATION OF SOME SCHIFF BASES DERIVED FROM 2-AMINOTHIAZOLE DERIVATIVES
Gupta, Rajul; Dept. of Pharmacy, CMJ University, Modrina Mansion, Laitumkhrah, Shillong, Meghalaya-793003, India.
Fuloria, Neeraj Kumar; Dept. of Pharmaceutical Chemistry, Anuradha College of Pharmacy, Chikhli, Dist. Buldana, Maharashtra, 443201, India.
Fuloria, Shivkanya; Dept. of Pharmaceutical Chemistry, Anuradha College of Pharmacy, Chikhli, Dist. Buldana, Maharashtra, 443201, India.
Various substituted acetophenones (1-5) on treatment with iodine and thiourea yielded 2-amino-4-(substituted-phenyl)thiazole (1a-5a), which on further treatment with various substituted aldehydes to get N-(substitutedbenzylidene)-4(substitutedphenyl)thiazol-2-amine (1b-5b). All the synthesized compounds were characterized by their respective FTIR, H NMR and Mass data. Synthesized compounds (1b-5b) were subjected to investigation for their antibacterial and antifungal studies against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Asperigillus flavus and Asperigillus fumigatus by disk diffusion method. Compound 5b was found to be most effective with largest zone of inhibition. Key words: Thiazole, Acetophenones Antibacterial, Antifungal, Substituted Aldehydes.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-09-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
text/html
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/513
10.14499/indonesianjpharm24iss1pp35-39
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 35-39
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/513/391
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/513/912
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1310
2018-11-02T06:46:53Z
3:ART
Derivatives of 3-(alkylthio)-5-(thiophen-2-ylmethyl)-4H-1,2,4-triazol-4-amines as increasing efficiency substances
Safonov, Andrey; Zaporizhzhia State Medical Univercity
1,2,4-triazole, pharmacological correction of fatigue, method of forced swimming
In today's society, especially in eastern countries such as China, Japan etc., the problem of fatigue and even death at work is acute. One of the solution to this problem is complex therapy with increasing efficiency drugs. Derivatives of 1,2,4-triazole have already proven themselves as potential compounds for pharmacological correction of fatigue. Compounds were synthesized at the Department of toxicological and inorganic chemistry ZSMU. Using method of forced swimming on a group of white nonlinear rats activity of anti-fatigue of the compounds were analyzed. Having analyzed the data of pharmacological correction of fatigue for 3-(alkylthio)-5-(thiophen-2-ylmethyl)-4H-1,2,4-triazol-4-amines, it should be noted that this series of compound do not show anti-fatigue effect. Introduction in molecule 3- (nonylthio)-5-(thiophen-2-ylmethyl)-4H- 1, 2, 4-triazol-4-amine 4-fluorbenzylidene, 4-(dimethylamino) benzylidene, 4- methoxybenzylidene, 2-chloro-6-fluorobenzylidene radicals lead to increase actoprotective effect. The most active compound is N-(2-chloro-6-fluorobenzylidene)-3-(nonylthio)-5-(thiophen- 2- ylmethyl)- 4H- 1, 2, 4- triazol- 4-amine which exceeds the standard (riboxin).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Zaporizhzhia State Medical University
2018-09-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1310
10.14499/indonesianjpharm29iss3pp167
Indonesian Journal of Pharmacy; Vol 29 No 3, 2018; 167
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1310/878
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1025
2017-09-11T04:29:11Z
3:ART
PHARMACEUTICAL PROPERTIES OF VENOM TOXINS AND THEIR POTENTIAL IN DRUG DISCOVERY
Kool, Jeroen; Division of BioAnalytical Chemistry, Department of Chemistry and Pharmaceutical Sciences, Faculty of Sciences, VU University Amsterdam, Amsterdam
Traditional pipelines feeding drugs coming to the market are declining. This is one of the reasons why nowadays the previously abandoned natural extract drug discovery programs are slowly coming back. In this scenario, small molecular metabolites from plants and single cell marine or soil organisms are gaining interest in pharmaceutical research again. Animal venoms are another source for finding new biopharmaceutical lead molecules and research interest in discovering bioactive molecules from venoms is rising. Venoms comprise often highly selective and potent bioactive peptides and small proteins for receptors and enzymes that are valid drug targets. This work discusses drug discovery research on bioactive compounds in venoms and gives older and more recent examples of bioactive compounds found in venoms from different animals. Common pharmaceutical targets that different classes of venom toxins interact with and information on developmental stages of several medicinal venom peptides are also discussed. Key words:venom, toxin, drug discovery, peptide, pharmaceutical activity
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1025
10.14499/indonesianjpharm27iss1pp1
Indonesian Journal of Pharmacy; Vol 27 No 1, 2016; 1
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1025/761
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1349
2019-10-10T01:29:33Z
3:ART
The Properties of Brown Marine Algae Sargassum turbinarioides and Sargassum ilicifolium Collected From Yogyakarta, Indonesia
Artemisia, Rahma; Stikes Madani Yogyakarta
Jl. Wonosari KM 10, Karanggayam, Sitimulyo, Piyungan, Bantul, DIY
Nugroho, Akhmad Kharis; Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
Setyowati, Erna Prawita; Department of Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
Martien, Ronny; Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
Sargassum turbinarioides, Sargassum ilicifolium, Fucoidan, Thin Layer Chromatography (TLC), Fourier transform infrared (FTIR) spectroscopy
Brown marine algae are the prominent source of marine natural products that have bioactive metabolites. Sargassum turbinarioides and Sargassum ilicifolium were dominated in Indonesia as brown marine algae that well known as a source of fucoidan. The samples were collected from Gunungkidul, Yogyakarta, Indonesia. In this study, we investigated and identified the yield of aqueous crude and purified extracts using different extraction temperatures (60°C, 70°C, 80°C, 90 °C). The highest yield of S. turbinarioides crude extract (7.36%) was obtained at temperatures 90 and 80 °C while the highest yield of S. ilicifolium was 3.49 % at 80 °C. The presence of sulfate polysaccharide in Sargassum turbinarioides is 3,78 % and Sargassum ilicifolium is 2,93 %. Each of the extract was screened using phytochemical detection, Thin Layer Chromatography (TLC) and Fourier transform infrared spectroscopy (FT-IR) analysis. The phytochemical detection indicated that Sargassum ilicifolium has bioactive metabolites such as carbohydrates, proteins and amino acids, terpenoid, phenolic compounds, and flavonoids. The FTIR spectrum of the S. turbinarioides and Sargassum ilicifolium extract refer to the presence of ester sulfate groups through showing peaks at 1300 to 1200 cm-1 and 980 to 950 cm-1. The result indicated that Sargassum turbinarioides and Sargassum ilicifolium contain of sulfate polysaccharide were prospect a biological activities to use for the development of marine nutraceutical drugs especially as antioxidant.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Ministry of Research, Technology, and Higher Education, Republic of Indonesia and also Beasiswa Unggulan Dosen Indonesia - Dalam Negeri (BUDI-DN) from Lembaga Pengelola Dana Pendidikan (LPDP) for sponsorship and financial support
2019-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1349
10.14499/indonesianjpharm30iss1pp43
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 43
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1349/892
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1182
2017-08-30T06:55:29Z
3:ART
Computer-aided Design of Chalcone Derivatives as Lead Compounds Targeting Acetylcholinesterase
Riswanto, Florentinus D. Octa; Faculty of Pharmacy, Sanata Dharma University, Yogyakarta, Indonesia.
Hariono, Maywan; Faculty of Pharmacy, Sanata Dharma University, Yogyakarta, Indonesia.
Yuliani, Sri Hartati; Faculty of Pharmacy, Sanata Dharma University, Yogyakarta, Indonesia.
Istyastono, Enade Perdana; Faculty of Pharmacy, Sanata Dharma University, Yogyakarta, Indonesia.
Computer-aided drug design, virtual screening, chalcone derivatives, acetylcholinesterase, Alzheimer’s disease.
One of well-established biological activities for chalcone derivatives is as acetylcholinesterase inhibitors, which can be developed for the therapy of Alzheimer’s disease. Assisted byretrospectively validated structure-based virtual screening (SBVS) protocol to identify potent acetylcholinesterase inhibitors, 80chalcone derivatives were designed and virtually screened. The F-measure value as the parameter of the predictive ability of the SBVS protocol developed in the research presented in this article was 0.413, which was considerably better than the original SBVS protocol (F-measure = 0.226). Among the screened chalcone derivatives two were selected as potential lead compounds to designpotent inhibitors for acetylcholinesterase: 3-[4-(benzyloxy)-3-methoxyphenyl]-1-(4-hydroxy-3-methoxyphenyl)prop-2-en-1-one(3k) and 3-[4-(benzyloxy)-3-methoxyphenyl]-1-(4-hydroxyphenyl)prop-2-en-1-one (4k).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1182
10.14499/indonesianjpharm28iss2pp100
Indonesian Journal of Pharmacy; Vol 28 No 2, 2017; 100
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1182/811
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1334
2019-10-10T01:29:33Z
3:ART
Profile of Biofilm-Producing Staphylococcus epidermidis from Intravenous Catheter Colonisation at Prof. Dr. Margono Soekarjo Hospital Purwokerto
Anjarwati, Dwi Utami; Jenderal Soedirman University
Siswandari, Wahyu; Dept of Pathology Clinic, Medical Faculty of Jenderal Soedirman University-Margono Soekarjo Hospital, Dr Gumbreg Nomor I, Medical Street, Mersi, Purwokerto, 53112
Peramiarti, IDSAP; Dept of Microbiology, Medical Faculty of Jenderal Soedirman University, Dr Gumbreg Nomor I, Medical Street, Mersi, Purwokerto, 53112
Biofilm, Intravenous catheter, colonization, Staphylococcus epidermidis
Biofilm- producing Staphylococcus epidermidis has evolved to be a significant human pathogen, particularly in the use of medical devices such as an intravenous catheter. Furthermore, biofilm-producing bacteria 10-1000 fold less susceptible to several antimicrobial agents than free-bacteria. This simple survey aimed to describe the profile of biofilm-producing S. epidermidis from intravenous catheter colonization of some patients in surgical and internal medicine wards at the hospital Margono Soekarjo, Purwokerto, and the antibiotics resistance pattern. A vitek® 2 compact (Enseval Medika Prima) was performed to identify the bacterial species and to examine the 73 antibiotics for understanding the resistance pattern automatically. Microtiter plate biofilm assay with crystal violet staining was performed to measure biofilm optical density (OD) for analyzing the biofilm production capabilities. A scanning electron microscopy (SEM) was done to compare the thickness of ultrastructure of biofilm-producing S. epidermidis visually. The present study found that 2 of 8 Gram-positive bacteria (25%) were biofilm-producing S. epidermidis. One of S. epidermidis was moderate whereas the other was high biofilm-producing bacteria. Images of SEM showed that a high biofilm-producing S. epidermidis has a thicker ultrastructure of biofilm than the moderate biofilm-producing, whereas a control, the weak biofilm-producing S. epidermidis ATCC 12228 has the least biofilm. Both of S. epidermidis strains were sensitive to Gentamicin, Moxifloxacin, Quinupristin/Dalfopristin, Linezolid, Vancomycin, Doxycycline, Minocycline, Tetracycline, Tigecycline, and Nitrofurantoin. Furthermore, both S. epidermidis strains were resistant to the other (63) antibiotics. In conclusion, two strains of S. epidermidis in this study have different capabilities to form the biofilm which were showed that high biofilm-producing strain was thicker than moderate biofilm-producing strain by scanning electron microscopy. However, both of them were resistant to the same number of antibiotics.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Prof. dr. Margono Soekarjo Hospital, Purwokerto
Research and higher education ministry, Indonesia
2019-03-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1334
10.14499/indonesianjpharm30iss1pp1
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 1
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1334/887
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1059
2017-09-11T05:04:51Z
3:ART
PHYTOCHEMICAL CONSTITUENTS AND HYPOGLYCEMIC EFFECT OF GYMNEMIC ACID EXTRACTS FROM BIG AND SMALL LEAF VARIETIES OF Gymnema Sylvestre R.Br
Krishnamurthy, Ramar; C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Bardoli, Surat, Gujarat, India
Animasaun, D. A.
Patel, R. T.
Ingalhalli, R. S.
Phytochemical and hypoglycemic effects of two varieties of Gymnema sylvestre were studied. The leaves of Big-leaf (MCL) and the small-leaf (ZMGL) varieties were grown in Uka Tarsadia University, Bardoli, India. Water, ethanol, methanol, petroleum ether, haxane and chloroform were used for phytochemical extraction from the dried leaves. Gymnemic acid was extracted and purified from the two varieties by Thin Layer Chromatography (TLC). Four groups of matured whisker rats (six per group) were induced with high-glucose level. Three groups were treated with standard drug Glibenclamide (5mg/kg), gymnemic acid extracts from MCL and ZMDL respectively while the fourth group served as experimental control. Hypoglycemic activities of extracts were evaluated using animals’ blood sample. Result showed water and methanol are best solvents for phytochemical extraction from the plant and the yield is higher in ZMGL than MCL. Also, gymnemic acid yield varied. From the results, hypoglycemic activities from the animals’ blood revealed that treatments from both MCL and ZMGL are not significantly different from standard drug Glibenclamide. The study concluded that water or methanol is appropriate solvent for phytochemical extraction from G. sylvestre leaves and extract from both lines of the plant could be utilized to reduce blood glucose levelKeywords: phytochemical extraction, extraction solvents, gymnemic acid, hypoglycemic effects
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-03-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1059
10.14499/indonesianjpharm27iss2pp59
Indonesian Journal of Pharmacy; Vol 27 No 2, 2016; 59
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1059/770
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1462
2019-10-10T01:30:23Z
3:ART
Effect of Platelet-rich Plasma on Caspase-3 and IGF-1 mRNA expression in the diabetic rat testis
Istiqamah, Evi; Postgraduate Student of Master Biomedical Science, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Department of Midwifery, Faculty of Public Health, Universitas Muslim Indonesia Makassar, Indonesia
Rizal, Dicky Moch.; Department of Physiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Puspitasari, Ika; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
PRP, diabetic rat testis, caspase-3 mRNA expression, IGF-1, mRNA in expression
Testicular damage is a serious complication of diabetes mellitus resulting in male infertility, which is associated with caspase-3 and IGF-1 mRNA expression. Platelet-rich plasma (PRP), with its rich growth factor composition, has proven beneficial in regenerative therapy. It is believed that PRP has not been studied in testes for diabetes mellitus and there are no studies in the literature concerning the influence of PRP on expressions of growth factors in testes.The aim of this study was to investigate the efficacy of adjunctive PRP in insulin treatment for repair of testicular damage in a diabetic rat model. Diabetes was induced by administering single dose 60 mg/kg streptozotocin. Twenty Wistar male rats were divided into four groups: group 1, control group; group 2, diabetes without treatment; group 3, diabetes with treated insulin; and group 4, diabetes with treated insulin and PRP. Rats were euthanized after two weeks of treatment, and testes were taken for caspase-3 and IGF-1 mRNA expression measurements.Diabetes mellitus induction caused a significant increase in caspase-3 mRNA expression with p=0.049 and significant decrease in IGF-1 mRNA expression with p=0.004. There was no difference in caspase-3 and IGF-1 mRNA expression of the diabetic rat testis given insulin and PRP compared to without PRP.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-05-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1462
10.14499/indonesianjpharm30iss2pp98-104
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 98-104
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1462/902
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/814
2017-09-12T07:00:50Z
3:ART
NARINGENIN-LOADED CHITOSAN NANOPARTICLES FORMULATION, AND ITS IN VITRO EVALUATION AGAINST T47D BREAST CANCER CELL LINE
Winarti, Lina; Dept of Pharmaceutics, Faculty of Pharmacy,
University of Jember,
Jember 68121, Indonesia
Ruma Kumala Sari, Lusia Oktora; Dept of Pharmaceutics, Faculty of Pharmacy,
University of Jember,
Jember 68121, Indonesia
Nugroho, Agung Endro; Dept of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas
Gadjah Mada, Yogyakarta 55281, Indonesia.
Naringenin (NAR), a natural flavonoid aglycone of naringin has been extensively investigated for its pharmacological activities, including anti-tumor effects. However, its poor bioavailability has been identified as the single most important challenge in oral drug delivery. Based in this condition, it is used nanoencapsulation to increase the effectiveness of NAR as anti-cancer. The objectives of this research are to develop the formulation of NAR-loaded nanoparticles (NARNPs) as well as to evaluate its potential as anti-cancer against T47D breast cancer cells line. NARNPs is prepared through the method of ionic gelation, meanwhile its characteristic is evaluated through photon correlation spectroscopy (PCS), transmission electron microscopy (TEM), fourier transform infra-red spectroscopy (FTIR), and different scanning calorimeter (DSC). The result of MTT test and cellular uptake indicate that NARNPs increase citotoxicity and internalization of NAR to the cells compared to the free NAR. The result of qualitative apoptosis study using fluorescence microscope indicates that both free NAR and NARNPs are able to induce apoptosis. It can be conclude that Chitosan nanoparticles–TPP conjugates have the capability to encapsulate naringenin hence increase the cellular uptake and cytotoxcicity of naringenin against T47D cell line. NARNPs also can induce the apoptosis effect.Keywords: NAR, Chitosan (CS), ionic gelation, nanoparticles
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2015-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/814
10.14499/indonesianjpharm25iss3pp147
Indonesian Journal of Pharmacy; Vol 26 No 3, 2015; 147
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/814/747
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1088
2017-08-02T06:45:06Z
3:ART
ANTIDIABETIC ACTIVITY OF ETHANOLIC EXTRACT OF KALANCHOE PINNATA LEAVES IN ALLOXAN INDUCED HYPERGLYCAEMIC RATS.
DD, Indah
Antidiabetic; Kalanchoe pinnata; hyperglycaemic; alloxan; pancreatic
Diabetes remains a major burden in health care both in developed and developing countries. Kalanchoe pinnata has been used as a traditional medicine to treat diabetes. We try to find scientific evidence of antidiabetic activity of Kalanchoe pinnata extract (KPE) through hypoglycemic effect using animal model of diabetes mellitus. Hyperglycaemia was developed in rats using alloxan 150 mg/kgBW. Three days after alloxan injection, rats having fasting blood glucose (FBG) >200 mg/dL were divided into six groups, namely HG (hyperglycaemia), HG+KPE high-dose (hyperglycaemia+KPE 33.2 mg/kg), HG+ KPE medium-dose (hyperglycaemia+KPE 11.6 mg/kg), HG+KPE low-dose (hyperglycaemia+ KPE 5.8 mg/kg), standard drug 1 (hyperglycaemia+glibenclamidee 1.35 mg/kg), standard drug 2 (hyperglycaemia+acarbose 13.5 mg/kg). Then, FBG was measured every 5 days recorded as t1, t2, and t3 to determine fluctuations in blood glucose. At the end of the study, rats were sacrified, pancreas was collected and number of pancreatic beta cell langerhans was determined. KPE 11.6 mg/kg showed best hypoglycemic effect and improvement of the number of pancreatic beta cell langerhans. KPE has hypoglycemic effect through improvement of the number of pancreatic beta cell langerhans but not in dose dependent manner.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1088
10.14499/indonesianjpharm27iss3pp139
Indonesian Journal of Pharmacy; Vol 27 No 3, 2016; 139
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1088/783
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/512
2018-09-05T01:49:54Z
3:ART
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/684
2020-01-27T02:53:31Z
3:ART
PHYSICAL PROPERTIES OF WOUND HEALING GEL OF ETHANOLIC EXTRACT OF BINAHONG (Anredera cordifolia (Ten) Steenis) DURING STORAGE.
Yuliani, Sri Hartati; Faculty of Pharmacy, Universitas Gadjah Mada,
Skip Utara, Yogyakarta 55281 Indonesia
Faculty of Pharmacy, Universitas Sanata Dharma, Paingan, Maguwoharjo, Depok, Yogyakarta 55284 Indonesia
Fudholi, Achmad; Faculty of Pharmacy, Universitas Gadjah Mada,
Skip Utara, Yogyakarta 55281 Indonesia
Pramono, Suwijiyo; Faculty of Pharmacy, Universitas Gadjah Mada,
Skip Utara, Yogyakarta 55281 Indonesia
., Marchaban; Faculty of Pharmacy, Universitas Gadjah Mada,
Skip Utara, Yogyakarta 55281 Indonesia
Binahong (Anredera cordifolia (Ten) Steenis) has been used as wound healing in traditional Indonesian medicine. The developing of the dosage forms using the formulation technology approach has been done. The aim of this study was to examine the physical properties of the wound healing gel of ethanolic extract of binahong during storage. The factorial design method 3 factors and 2 levels was employed to achieve this study. The three factors used in this study were Carbopol, CMC-Na and Caalginate with low and high level for each factor. The physical properties of the wound healing gel of ethanolic extract of binahong was evaluated on day 1, 30, 60 and 90 for viscosity and bioadhesion study. The result showed that CMC-Na and Caalginate increased the alteration of physical properties of wound healing gel of ethanolic extract of binahong during storage. The carbopol maintained the physical properties of the gel during storage.Key words: bioadhesion, binahong, viscosity, wound healing gel.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/684
10.14499/indonesianjpharm23iss4pp203-208
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 203-208
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/684/550
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1058
2017-09-11T06:10:15Z
3:ART
FORMULATION AND EVALUATION OF TRANSDERMAL PATCHES OF ATENOLOL
Budhathoki, Uttam; Department of Pharmacy, Kathmandu University, Dhulikhel, Kavre, Nepal. GPO box 6250 (Kathmandu)
Gartoulla, Kshitij; Department of Pharmacy, Kathmandu University, Dhulikhel, Kavre, Nepal. GPO box 6250 (Kathmandu)
Shakya, Shailendra; Department of Pharmacy, Kathmandu University, Dhulikhel, Kavre, Nepal. GPO box 6250 (Kathmandu)
Atenolol; Transdermal Patches; In-Vitro permeation study; permeation enhancer; HPMC; PVP
This study was carried out to develop matrix based transdermal patches containing Atenolol. A 2 factors (HPMC (hydroxyl propyl methyl cellulose) K4M & PVP (Polyvinyl Pyrolidone) 3 level (23) factorial design was done using Design Expert® which gave 13 experiments. The patches were prepared by Solvent casting method. Propylene glycol (3%) and Tween 80 (6%) were used as plasticizer and permeation enhancer respectively. Physicochemical characteristics and In-Vitro permeation study of formulated transdermal patches were carried out. Contour plot suggested 770 mg of PVP and 265 mg of HPMC K4M in Optimized formulation.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-12-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1058
10.14499/indonesianjpharm27iss4pp196
Indonesian Journal of Pharmacy; Vol 27 No 4, 2016; 196
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1058/793
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1564
2022-10-26T01:05:53Z
3:ART
Response Surface Methodology used in the Optimization of RP-HPLC Condition for Quantitative Analysis of Carmine and Rhodamine B
Nuvitasari, Reyna
Rohman, Abdul; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Martono, Sudibyo
The objective of this study was to optimize reversed-phase high-performance liquid chromatography (RP-HPLC) using an experimental design approach based on the response surface methodology of Central Composite Design (CCD) for separation and analysis of carmine (CAR) and Rhodamine B (RHO) in lipstick products. Some factors (independent variables) responsible for RP-HPLC separation including pH of buffer phosphate (X1), the acetonitrile ratio (X2), flow rate of mobile phase (X3), and column temperature (X4) were investigated. While, the responses (dependent variables) evaluated were resolution between CAR and RHO (Y1), tailing factor of CAR (Y2), tailing factor of RHO (Y3), retention time of CAR (Y4), retention time of RHO (Y5), peak area of CAR (Y6) and peak area of RHO (Y7). CCD showed that separation of CAR and RHO was influenced by these independent variables (factors). The optimum predicted conditions for the separation of CAR and RHO based on statistical results was pH buffer of 3.4, ACN 55%, the flow rate of 1.1 mL/min and column temperature of 35oC with the desirability of 1. Both CAR and RHO were clearly separated using optimum conditions, as suggested by CCD. The developed techniques were effective for optimizing chromatographic separation, therefore, the time consumption and a large number of running could be hindered.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-10-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1564
10.14499/indonesianjpharm30iss4pp276
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 276
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1564/922
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/508
2020-02-19T02:37:07Z
3:ART
AKTIVITAS PENGANGKAPAN RADIKAL POLIFENOL DALAM DAUN TEH
Rohdiana, Dadan; Tea and Quinine Research Center
Gambung PO BOX 1013 Bandung 40010
Ability of free radical scavengers of various tea extracts (black tea, fermented tea; green tea , unfermented tea; oolong dan pouchong tea semifermented tea) was studied. The scavenging effects on active oxygen decreased in order semifemented tea > unferfmnted tea > fennented lea. Antioxidant activity on tea often might related wthi catechin presence. Catechin activity as antioxidant estabilished. Several epidemilogical and phartnacokgical research shown that catechin had a strongly antioxidan activity. (-)-epigallocatechin gallate (EGCG) and (-)-epicatechin gallate (ECG) are the major catechin of ~ tea This compounds had a strongly scavenging activity on free radical such as superoxide, hydrogen superoxide, DPPH and peroxynitdte. This review aim to exploited~ tea Oyphenols ability as free radicals sw vengers Key word: antioxidant activity, active oxygen, scavenging effect
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/508
10.14499/indonesianjpharm0iss0pp52-58
Indonesian Journal of Pharmacy; Vol 12 No 1, 2001; 52-58
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/508/386
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1329
2018-10-23T02:19:33Z
3:ART
Effects of Probiotics and Vitamin B Supplementation on IFN-γ and IL-12 Levels During Intensive Phase Treatment of Tuberculosis
Suprapti, Budi; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Suharjono, Suharjono; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Raising, Rahmawati; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Yulistiani, Yulistiani; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Izzah, Zamrotul; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Nilamsari, Wenny Putri; Department of Clinical Pharmacy, Universitas Airlangga, Surabaya
Wulaningrum, Prastuti Asta; Department of Pulmonology and Respiratory Medicine, Universitas Airlangga Teaching Hospital, Surabaya
Bachtiar, Arief; Department of Pulmonology and Respiratory Medicine, Universitas Airlangga Teaching Hospital, Surabaya
Interleukin-12, Interferon-γ, Probiotics, Tuberculosis, Vitamin B
Tuberculosis is an acute infectious disease that primarily affects the lungs. Probiotics supplementation can increase the number and activity of NK cell in peripheral blood by modulation of IL-12, thus increasing IFN-γ production by Th1 response. Vitamin B1 acts on macrophages and affects neutrophil motility. Vitamin B6 is associated with the release of cytokines and the responsiveness of NK cells, while vitamin B12 affects to lymphocytes, Tcell proliferation, CD4+ ratios, and NK cell activity. To analyze the effects of probiotics and vitamin B1, B6, B12 supplementation on IFN-γ and IL-12 levels during intensive phase of antituberculosis treatment. The study was pre-post test randomised control by time series. The control group was TB patients with standard therapy of antituberculosis and vitamin B6, while the intervention group was TB patients receiving therapy plus once daily probiotics and vitamin B1, B6, B12supplementation during the intensive phase. Blood samples were withdrawn at baseline, one month, and two months after therapy to measure plasma IFN-γ and IL-12 levels using the ELISA method. Twenty two patients were divided equally into two groups. There was a tendency to greater increase of IFN-γ in the first month of the intervention group, followed by a significant decline after two-month therapy (p < 0.05). In both groups there was a rise in IL-12 levels after one month followed by a decrease in the second month (p > 0.05). However, the percentage was higher in the supplementation group. Adding probiotics and vitamins B1, B6, B12 could improve immune response through IL-12 and IFN-γ modulation during intensive phase therapy.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-06-22
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1329
10.14499/indonesianjpharm29iss2pp80
Indonesian Journal of Pharmacy; Vol 29 No 2, 2018; 80
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1329/868
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/855
2017-09-11T04:54:08Z
3:ART
SUN PROTECTOR FACTOR (SPF) IN VITRO AND THE PHYSICAL STABILITY OF O/W CREAM OPTIMAL FORMULA FROM THE PARTITION PRODUCT OF MAHKOTA DEWA LEAVES [Phaleria macrocarpa (Scheff) Boerl]
Zulkarnain, Abdul Karim; Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta
Marchaban, Marchaban; Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta
Wahyuono, Subagus; Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta
Susidarti, Ratna Asmah; Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta
The leaves Mahkota dewa (Phaleria macrocarpa (Scheff) Boerl) contains a benzophenoic glycoside (Phalerin) which is active for sun screen. Some formula and designed on the Simplex Lattice Design (SLD) method then its physical characteristics were investigated. The physical characteristics will be used to determine the optimum formula. In present study, the optimum formulation was evaluated to determine chemical stability and the in vitro SPF. The doses of partition product of P. macrocarpa was used 6%. The optimal formulation cream was determined by SLD version 9.0.1 with variety of Cetyl alcohol, mineral oil, and Tween 80. The difference of physical characteristics between experimental and software was analyzed using T-test, P= 95 %. The physical stability and in vitro SPF of the optimum formula was observed. he study exhibits that the combination of cetyl alchodol 9,71%, mineral oil 29%, and tween 80 3,29% was the optimum formula of o/w cream with the desirability test 0,924. The T-test result test shows that there is no significant difference (p>0.05) on physical characteristics between experimental and software prediction. During the storage, the viscosity, spread ability, adhesive ability, separation volume ratio of cream was relatively stable however the stability started to decline on the week IV in the extreme temperature. The SPF of the cream of the CFR, CPHR and CBZP partition were 21,32; 33,12 and 42,49, respectively.Keywords: Mahkota dewa, cream, optimum, SPF
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2015-11-13
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/855
10.14499/indonesianjpharm26iss4pp210
Indonesian Journal of Pharmacy; Vol 26 No 4, 2015; 210
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/855/756
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1130
2018-01-09T08:39:19Z
3:ART
Evaluation of In vitro and In vivo Antioxidant potential of Morinda reticulata Gamble Tubers in Wistar Albino Rats Subjected to CCl4 and Paracetamol induced Hepatotoxicity
Asirvatham, Raju
Usha, Joshila Jose
M.reticulata; in vitro and in vivo antioxidant; DPPH; Sylimarin; Melondyaldehyde (MDA); Reduced Glutathione (GSH); Super oxide dismutase (SOD)
The aim of present study is to explore the antioxidant potential of ethanol and aqueous extracts of Morinda reticulata Gamble by in vitro and in vivo methods. In vitro antioxidant activity of Benzene, Chloroform, Ethanol and aqueous extracts of M. reticulata was studied by DPPH, Metal chelating, Super oxide free radical scavenging assay, Hydroxyl radical scavenging assay and Reducing power assay where ascorbic acid was used as a standard antioxidant. Oxidative stress in Wistar rats was induced by two different models using administration of CCl4 (1.5 ml/kg, p.o) and paracetamol (2g/kg) on seventh day of study. Ethanol and aqueous extracts were given twice daily for one week. Silymarin (25 mg/kg, p.o) was given as a standard drug. In in vitro, IC50 values were least with ethanol and aqueous extracts when compared with other extracts. Ethanol extracts exhibited similar free radical scavenging effect as that of standard ascorbic acid. In vivo antioxidant activity was assessed by the measurement of Melondyaldehyde (MDA), Reduced Glutathione (GSH), Super oxide dismutase (SOD) and Total protein levels were estimated from the liver tissue homogenate. The level of MDA (3.27±2.18) was significantly (p˂0.001) increased whereas decreased level of total protein, GSH and SOD were found in CCl4 and paracetamol control group. Seven days extracts treatments restored these altered parameters in to normal where ethanol extract treatment group showed significant (p˂0.001) result than aqueous extract. HPTLC study showed that presence of six phytoconstituents with corresponding Rf value. The study report concluded that M.reticulata has very good antioxidant effect against CCl4 and paracetamol induced liver damage with oxidative stress. Probable mechanism behind this protection against oxidative damage produced by CCl4 and paracetamol is its phytoconstiuents.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-08-08
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1130
10.14499/indonesianjpharm28iss3pp147
Indonesian Journal of Pharmacy; Vol 28 No 3, 2017; 147
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1130/832
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1130/108
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1903
2021-01-06T13:13:53Z
3:ART
In Vitro Antiplasmodial Activity and Cytotoxicity of Active Subfractions of Harmsiopanax aculeatus Leaves
Turalely, Rachel; Biotechnology Study Program, Graduate School, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Chemistry Education Study Program, Faculty of Teacher Training and Education Science, Pattimura University, Ambon, Maluku, Indonesia
Wijayanti, Mahardika Agus; Department of Parasitology, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada,
Hertiani, Triana; Faculty of Pharmacy, Universitas Gadjah Mada
Mustofa, Musthofa; Department of Pharmacology and Therapy, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada Yogyakarta, Indonesia.
H. aculeatus, antiplasmodial activity, cytotoxicity, malaria, subfractions
Harmsiopanax aculeatus leaves, a medicinal plant with locally named kapur, have been used traditionally to treat malaria in Maluku, Indonesia. However, the scientific information of this plant is still limited. In our previous study, the methanol extract of this plant leaves have been proven to possess in vitro antiplasmodial activity. This study was conducted to evaluate in vitro antiplasmodial activity and cytotoxicity of subfractions of the plant leaves. Fractionation was performed using a column chromatography with Sephadex LH-20 as the stationary phase and methanol as the mobile phase. The subfractions obtained were then tested for in vitro antiplasmodial activity on a chloroquine-resistant FCR3 strain of Plasmodium falciparum using a visual method. Cytotoxicity was evaluated by using MTT assay. The in vitro antiplasmodial activity and cytotoxicity were expressed as IC50, calculated using probit analysis with SPSS 16 for windows. The results showed that the four subfractions tested have a high antiplasmodial activity with IC50 values of 0.09; 0.18; 0.01; and 0.77 µg.mL-1, respectively. In addition, these subfractions had IC50 values of >400 µg.mL-1 against Vero cells indicating that they were non-toxic. In conclusion, the subfractions of H. aculeatus leaves are very active and selective against P. falciparum. Further study will be conducted to isolate the active compounds.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-04-09
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1903
10.14499/indonesianjpharm31iss1pp51
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 51
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1903/944
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/667
2020-01-27T02:51:49Z
3:ART
THE OPTIMISED CONDITIONS OF INDUCTION OF RECOMBINANT RIP rMJC15310 ACTIVITY ISOLATED FROM Mirabilis jalapa L. LEAVES
Astuti, Puji; Department of Pharmaceutical Biology,
Faculty of Pharmacy Universitas Gadjah Mada,
Sekip Utara, Yogyakarta, 55281.
., Sudjadi; Department of Pharmaceutical Chemistry, Faculty of Pharmacy Universitas Gadjah Mada, Sekip Utara, Yogyakarta, 55281
., Sismindari; Department of Pharmaceutical Chemistry, Faculty of Pharmacy Universitas Gadjah Mada, Sekip Utara, Yogyakarta, 55281
Ribosome Inactivating Proteins (RIPs) are compounds isolated from plants with ability to inhibit protein synthesis. The inhibition of protein synthesis is due to inactivation of ribosomal RNA through a site-specific deadenylation mediated by RNA N-glycosidase. Reportedly, RIPs mainly possess wide range of bioactivity including antiviral activity against plant infections. Other activities of RIP were as abortifacien, antivirus and anticancer. This study was aimed to isolate and characterize the optimum conditions for inducing the expression of recombinant RIPs isolated from the leaves of Mirabilis Jalapa L. We have been successfully isolated several RIPs and engineered these proteins to be expressed in E. coli. These recombinant proteins were obtained by screening cDNA library originated from the mRNA of Mirabilis jalapa L leaves, and inserted into pUC19 carrying lacZ gene. The presence of recombinant plasmid was tested by using α-complementation assay. Many RIPs have been isolated from plants and these proteins express enzymatic activity by cutting supercoiled double stranded DNA. One RIP namely rMJC15310 was obtained from this study and the proteins having ~ 8kb in size, cut the supercoiled DNA into linear form at the concentration as low as 5 µg. The ability to cut supercoiled DNA increased on inducing its expression with 0.4% IPTG.Key words: Ribosome Inactivating Proteins (RIP), IPTG, Mirabilis jalapa L., recombinant protein
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/667
10.14499/indonesianjpharm23iss2pp93-98
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 93-98
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/667/533
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1521
2019-10-10T01:30:23Z
3:ART
The Effect of Thionamide to TRH, TSH, IL-4, T-REG, and Anti-TPO in Graves’ Disease
Decroli, Eva; Endocrinology and Metabolic Subdivision, Division of Internal Medicine, Dr. M. Djamil General Hospital/ Medical Faculty of Andalas University, Padang, West Sumatera, Indonesia
Elvira, Dwitya; Allergy and Immunology Subdivision, Division of Internal Medicine, Dr. M. Djamil General Hospital/ Medical Faculty of Andalas University, Padang, West Sumatera, Indonesia
Aprilia, Dinda; Endocrinology and Metabolic Subdivision, Division of Internal Medicine, Dr. M. Djamil General Hospital/ Medical Faculty of Andalas University, Padang, West Sumatera, Indonesia
TRH, TSH, T-reg, IL-4, anti-TPO
The most common cause of hyperthyroidism is Graves' disease. TRH and TSH are hormonal factors that modulate and control thyroid function in Graves' disease. In the immunological aspect, Graves' disease is played by the role of T-reg, IL-4, and anti-TPO. Graves' disease treatment goal is to inhibit thyroid hormone secretion by administering thionamide. The evaluation of this treatment is its hormonal and immunological aspects. To describe the effect of thionamide on serum TRH, TSH, IL-4, T-reg, and anti-TPO levels in Graves' disease. This study is a clinical trial study in 25 study participants. All study participants were given thionamide, namely PTU 300mg for three months and blood samples were taken for laboratory tests. Serum TRH, TSH, IL-4, T-reg FOXP3, and anti-TPO levels were examined by ELISA. The mean levels at the beginning and after three months of therapy are: serum TRH 92.589pg/mL and 115.944pg/mL; serum TSH 0.041mU/L and 0.223mU/L; serum IL-4 19.759pg/mL and 23.040pg/mL; T-reg FOXP3 gene polymorphism 0.621ng/mL and 0.518 ng/mL; serum anti-TPO 2697.539pg/mL and 2604.710pg/mL. Increased levels of serum TRH and TSH levels were statistically significant. The change in serum IL-4, T-reg FOXP3 gene polymorphism, and anti-TPO levels were not statistically significant. The administration of thionamide in Graves' disease for three months will significantly decrease Wayne index and serum FT4 levels, increase serum TRH and TSH levels.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-05-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1521
10.14499/indonesianjpharm30iss2pp122-127
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 122-127
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1521/897
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/811
2017-09-12T07:25:03Z
3:ART
ANTIHYPERGLYCEMIC, ANTIOXIDANT, AND PANCREAS PROTECTIVE EFFECTS OF CORIANDRUM SATIVUM SEED IN ALLOXAN-INDUCED DIABETIC RATS
Widodo, Gunawan Pamudji; Faculty of Pharmacy, Setia Budi University, Surakarta, Indonesia.
Jalan Letjen Sutoyo Mojosongo, Surakarta, 57127. Indonesia
Handayani, Sri Rejeki; Faculty of Pharmacy, Setia Budi University, Surakarta, Indonesia.
Jalan Letjen Sutoyo Mojosongo, Surakarta, 57127. Indonesia
Hierowat, Rina; Faculty of Pharmacy, Setia Budi University, Surakarta, Indonesia.
Jalan Letjen Sutoyo Mojosongo, Surakarta, 57127. Indonesia
Coriandrum sativum is a medicinal plant, used in traditional medicine for diabetes therapy. The goal of the this study was to determine the antidiabetic, antioxidant and pancreas protection effects of ethanol extracts of C. sativum seeds (CSE) in alloxan-induced diabetic rats. The male Wistar rats were induced diabetic by intraperitoneal injection of alloxan (150mg/kg BW). CSE was prepared and administrated orally to the animals at the dose of 125 and 250mg/kg for 28 days. Blood glucose level was measured, and antioxidant status was assessed by determining the activities of superoxide dismutase, glutathione peroxidase as well as malonyl aldehyde in liver. Histopathological study of pancreas was conducted at the end of experimental period. Both dose of SCE showed the glucose lowering effect, corrected antioxidant status of diabetic animals in liver and protected the pancreas organ from damage. Key words: Coriandrum sativum seed, antihyperglycemia, antioxidant, pancreas protection
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2015-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/811
10.14499/indonesianjpharm25iss3pp129
Indonesian Journal of Pharmacy; Vol 26 No 3, 2015; 129
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/811/750
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1107
2017-08-30T06:55:29Z
3:ART
The Effect of Medication Reminder Chart on Level Adherence in diabetes mellitus type 2 patients at RSUD Sleman Yogyakarta
Suffiana, Yunita; Gadjah Mada University
Rahmawati, Fita; Gadjah Mada University
Andayani, Tri Murti; Gadjah Mada University
Compliance, Clinical outcome, MMAS-8, Medication Reminder Chart
Patient adherence is a key factor that determines the outcome of diabetes mellitus therapy. This study was conducted to determine the effect of a medication reminder chart on level adherence and clinical outcome in patients with Type 2 diabetes mellitus. The experimental study used pretest-posttest design with control group was conducted during February until April of 2016 at Sleman Hospital in Yogyakarta. Patients were categorized into two groups of different subjects, who received of medication reminder chart tools were 40 patients (Code A) and the other 40 patients were assigned to control group (Code B). Morisky Medication Adherence Scale of 8-item (MMAS-8) was used to measure adherence. Clinical outcome were measured based on FBG decrease (mg/dl). Two independent sample t-test was using to determine the mean differences of the average score of the adherence level before and after using the medication reminder chart. The results of this study showed that Medication Reminder Chart compared control groups improved adherence (p=0.000) but did not improve clinical outcome (p=0.411). The pharmacist might use The Medication Reminder Chart to improve medication adherence for chronic disease.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-06-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1107
10.14499/indonesianjpharm28iss2pp125
Indonesian Journal of Pharmacy; Vol 28 No 2, 2017; 125
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1107/808
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/950
2017-08-01T04:08:57Z
3:ART
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/679
2020-01-27T02:53:16Z
3:ART
QSAR MODELING OF 2-[CH(OH)X]-5,8-(OY)2 -1,4-NAPHTHOQUININES AGAINST L1210 CELLS USING MULTIPLE LINEAR REGRESSION
., Ajeet; Department of Pharmaceutical Chemistry,
S. D. College of Pharmacy and Vocational Studies, Muzaffarnagar, U.P. 251001
India
Singh, Brajpal; Department of Pharmaceutical Chemistry,
S. D. College of Pharmacy and Vocational Studies, Muzaffarnagar, U.P. 251001
India
Kumar, Vipul; Department of Pharmaceutical Technology, Meerut Institute of Engineering and Technology, Meerut, U.P., India
Quinones are present in many drugs such as anthracyclines, daunorubicin, doxorubicin, mitomycin, mitoxantrones and saintopin, which are used clinically in the therapy of solid cancers. The cytotoxic effects of these quinone are mainly due to the inhibition of DNA topoisomerase-II. It is the necessity to develop the 1,4-Naphthoquinone analogues with Cytotoxic effect. Here 2-[CH(OH)X]-5,8-(OY)2-1,4-Naphthoquinines analogues have been used to correlate the cytotoxic activity with the Eccentric Connectivity index (ECI), Fragment Complexity (FC) and McGowan Volumes (MG) for studying the Quantitative Structure Activity Relationship (QSAR). Correlation may be an adequate predictive model which can help to provide guidance in designing and subsequently yielding greatly specific compounds that may have reduced side effects and improved pharmacological activities. We have used Multiple Linear Regression (MLR), one of the best methods for developing the QSAR model. Results from this QSAR study have suggested that ECI, FC and MG are the important descriptors for cytotoxic activities of 1,4-Naphthoquinones against L1210 cells. For the validation of the developed QSAR model, statistical analysis such as data point-descriptor ratio, fraction of variance, cross validation test, standard deviation, quality factor, Fischer’s test; and internal validation such as Y-randomization test have been performed and all the tests validated this QSAR model.Key words: 1,4-Naphthoquinones, QSAR, Eccentric connectivity index, Fragment complexity, McGowan Volume, Multiple Linear Regression
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/679
10.14499/indonesianjpharm23iss3pp171-176
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 171-176
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/679/545
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1120
2017-08-28T03:41:06Z
3:ART
NATIONAL HEALTH COVERAGE SYSTEM : PHARMACISTS AND JKN PARTICIPANT SATISFACTION IN PRIMARY HEALTH FACILITIES
Satibi, Satibi; Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara 55281
National Health Insurance is a health protection system for participants to obtain health care benefits and protection to meet basic health needs that organized based on social insurance and equity principles. JKN in cooperation with the primary health facilities to provide comprehensive health services including pharmacy services. Pharmacist responsible for drug availability and affordability that affected by drug procurement process, drug distribution process, and drug cost claiming process.This research’s aim to determine relationship between national health insurances services to pharmacists satisfaction and to determine relationship between pharmacy services to outpatient satisfaction at primary health facilities. Data used in research are primary data that collected through questionnare and interview. The method used is purposive sampling which the data is analyzed using linear and multiple regresion and see significant value (p). The number of respondents was 40 pharmacists and 150 patients in health centers and primary clinics scattered in Sleman, Bantul, and Kota Yogyakarta. The results showed that the process of procurement and distribution of drugs have a relationship with a pharmacist satisfaction while drug costs not process claims with consecutive p 0.010, 0.002, and 0.261. Related to the satisfaction of the outpatient pharmacy services result there is a relationship of drug availability (p = 0.003), service time (p = 0.006), and get drug (p = 0.000) to the satisfaction of outpatients. The conclusion of this study is the process of procurement and distribution of drugs have a relationship with a pharmacist satisfaction. In addition, the availability of pharmacy services such as medication, a drug services, and the provision of drug information also has a significant relationship to the satisfaction of outpatients in health facilities first rate.Keywords: pharmacy services, pharmacists, patients, JKN
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-09-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1120
10.14499/indonesianjpharm27iss4pp231
Indonesian Journal of Pharmacy; Vol 27 No 4, 2016; 231
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1120/798
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1468
2019-10-28T01:20:02Z
3:ART
Antibacterial Compound from Aspergillus elegans SweF9 an Endophytic Fungus from Macroalgae Euchema sp.
Mulyani, Hani; 1. Pharmacy Faculty, Pancasila University,Jl. Raya Lenteng Agung Timur No.56-80, Srengseng Sawah, Jagakarsa, Jakarta
2. Indonesian Institute of Sciences (LIPI)
Dewi, Rizna Triana; Indonesian Institute of Sciences (LIPI)
Chaidir, Chaidir; Pharmacy Faculty, Pancasila University,Jl. Raya Lenteng Agung Timur No.56-80, Srengseng Sawah, Jagakarsa, Jakarta
Aspergillus elegans SweF9, antibacteria, (+)-epi-Epoformin.
The antibiotic resistance of bacterial pathogens has become a serious health concern and encouragement to search for novel and efficient antimicrobial metabolites. On the other hand, endophytic fungi have great potential as a natural source for antimicrobial agents. The objective of this study was to isolation antibacterial compound from endophytic fungi of A.elegans SweF9. The fungus was stationarily cultured at 30°C for 12 days in potato malt peptone (PMP) medium, then extracted with ethyl acetate. The antibacterial activities of the extract were evaluated by agar well diffusion method against Gram-poitive (Bacillus subtilis and Staphylococcus aureus) and Gram-negative (Escherichia coli) bacterial strains. The broth extract was able to inhibit the growth of E. coli, S. aureus and B. subtilis with antibacterial activity index compared to streptomycin sulfate were 84.6%, 91.6%, and 90% respectively. The active compound (1) was purified to yield amorphous white and identified using FTIR, NMR, and EI-MS analyses, revealed identified as (+) - epi-Epoformin. The compound showed an antibacterial activity index of E. coli, S. aureus and B. subtilis bacterial were 38%, 45%, and 47%, respectively. Based on these results endophytic fungi A. elegans SweF9 can be used as a new source of potential antibacterial compounds
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Indonesian Institute of Sciences
2019-09-20
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1468
10.14499/indonesianjpharm30iss3pp217
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 217
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1468/916
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/442
2020-02-19T02:37:07Z
3:ART
A COMPARATIVE BIOAVAILABILITY OF FUROSEMIDE IN SOLID DISPERSIONS FORMS
Syukri, Yandi; Departement of Pharmacy
Faculty of Science and Mathematic
Islamic Indonesa University
Hakim, Lukman; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
Yuwono, Tedjo; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
Furosemide is a poorly soluble diuretic drug, the solubility of which can be enhanced by solid dispersion with polyvinylpirolidon (PVP). The solid dispersion system was prepared by a solvent method in various ratios of 1 : 5 and 1 : 7 of the drug and PVP, respectively, in order to improve furosemide bioavailability. The bioavailability of furosemide - PVP solid dispersion was compared with pure furosemide (control) and Lasix (reference). The study was done in a cross over design with a single-dose peroral that administered to the white male rabbits (n = 6). Furosemide blood levels were determined spectrofluorometrically by an extraction method. The area under the blood concentration-time curve AUC0 - , peak blood concentration Cmax and time to reach peak blood concentration Tmax were used to compare their bioavailabilities. The solid dispersion systems produced a higher extent of bioavailability than pure furosemide (P < 0,05). On the contrary, no statistically significant difference about the extent of bioavailability between solid dispersion and Lasix (P > 0,05). Finally, furosemide – PVP solid dispersion (1 : 7) was the best formulation with the highest extent of bioavailability and bioequivalence with the Lasix formula.Key Word : Solid dispersion, solubility, dissolution, bioavailability, furosemide.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/442
10.14499/indonesianjpharm0iss0pp28-32
Indonesian Journal of Pharmacy; Vol 12 No 1, 2001; 28-32
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/442/321
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1289
2018-10-23T02:19:20Z
3:ART
In Vitro Evaluation of Anti-Inflammatory and Anti-Diabetic Potential Effects of Euphorbia Tithymaloides Ethanol Extract
Wandita, Theresia Galuh; Departement of Animal Life and Environmental Science, HANKYONG NATIONAL UNIVERSITY, SOUTH KOREA
Joshi, Najuma; Departement of Animal Life and Environmental Science, HANKYONG NATIONAL UNIVERSITY, SOUTH KOREA
dela Cruz, Joseph Flores; Department of Basic Veterinary Sciences
College of Veterinary Medicine
University of the Philippines Los Banos, PHILIPPINES
Hwang, Seong Gu; Departement of Animal Life and Environmental Science, HANKYONG NATIONAL UNIVERSITY, SOUTH KOREA
Hibiscus rosasinensis, glipizide, mucilage, antidiabetic activity, excipients
This research work aims to develop glipizide tablets using Hibiscus rosasinensis leaves mucilage. The mucilage was extracted by using double distilled water and precipitated with ethanol. The precipitated mucilage was dried and grounded into powder. The tablet utilizing the mucilage as excipient was prepared by wet granulation method. The tablets were subjected to various tests. The evaluatory parameters of tablets were found to be within the limits as per United States Pharmacopoeia NF 24/19. FTIR spectrum reveals that there is no incompatibility between the ingredients used. Diabetes was induced in wistar albino rats using streptozotocin and effect of formulation on blood glucose level was determined. It was observed that the formulation could not sustain the release of glipizide. However, the glipizide release was retarded as the amount of mucilage was increased. It was observed that on the completion of antidiabetic study, the formulation could bring the blood glucose level to normal in group rats. However, the blood glucose level was still elevated in group administered with pure gliplizide. It can be concluded that HRM can be used to formulate glipizide tablets. The formulations with HRM shows better hypoglycemic activity and this may be attributed to antidiabetic activity of Hibiscus rosasinensis.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-03-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1289
10.14499/indonesianjpharm29iss1pp1
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 1
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1289/858
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/949
2017-09-11T06:12:36Z
3:ART
ANTIDIABETIC EFFECT EVALUATION AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS ISOLATED FROM HYDROLEA ZEYLANICA
S, Borkar Vijay; Padmavathi College of Pharmacy and Research Institute, Dharmapuri (TN) India.
571/2/3/1 Davkhar Nagar, Chandwad (Nasik) Maharashtra, India- 423101.
K, Senthil Kumaran; K.K. College of Pharmacy, Chennai (TN) India.
KL, Senthil Kumar; Padmavathi College of Pharmacy and Research Institute, Dharmapuri (TN) India.
H, Gangurde Hemant; SSDJ College of Pharmacy, Chandwad (MS) India.
A, Chordiya Mayur; SSDJ College of Pharmacy, Chandwad (MS) India.
The whole plant of Hydrolea zeylanica (HZ) (Hydrophyllaceae family) was coarsely powdered and extracted with ethanol using soxhlet apparatus. The ethanol extract was then fractionated successively using various polarity ranges of solvents and screened for in-vivo antidiabetic activity using streptozotocin induced diabetic male wistar rats. The phytochemical investigation of all the fractions and powdered drug analysis was performed. Among the fractions evaluated, chloroform fraction showed highest decrease in blood glucose, total cholesterol and serum triglyceride level as 75.11%, 59.77% and 35.98% respectively when treated at 50 mg/mL concentration. As the chloroform fraction has shown better potency towards antidiabetic activity, was subjected to chromatographic separation and three compounds stigmasterol, kaempferol and p- coumaric acid were isolated and characterized by various spectroscopic techniques. The overall results tend to suggest the antidiabetic activity of HZ and principal source of presumed bioactive compounds which may be responsible for many of the pharmacological properties.Key word: Hydrolea zeylanica, antidiabetic, total cholesterol, triglycerides, in vivo.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2015-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/949
10.14499/indonesianjpharm26iss4pp185
Indonesian Journal of Pharmacy; Vol 26 No 4, 2015; 185
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/949/741
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1226
2018-10-23T02:18:57Z
3:ART
Provision of Patient Medication Counseling Service In A Tertiary-Care Eye Hospital In Bandung: A Gap Analysis of Patient’s Need, Standard and Service Provider Resources
Hendrayana, Tomi; Department of Pharmacology-Clinical Pharmacy, School of Pharmacy, Bandung Institute of Technology
Zazuli, Zulfan; Department of Pharmacology-Clinical Pharmacy, School of Pharmacy, Bandung Institute of Technology
Fadhila, Genialita; Department of Pharmacology-Clinical Pharmacy, School of Pharmacy, Bandung Institute of Technology
Cahyono, Nur Sidik; Department of Pharmacology-Clinical Pharmacy, School of Pharmacy, Bandung Institute of Technology
Provision of new service; patient medication counseling service; gap analysis; tertiary-care eye hospital; pharmaceutical care
Many studies showed that counseling can improve patient adherence and reduce medication-related problems leading to reach an optimal outcome therapy. The aims of this study were to identify patient’s need, healthcare professional’s support, pharmacist’s readiness and step to be taken to meet standard for implementing patient medication counseling service. Questionnaires has been given to hospitalized-patient to identify the patient’s need and to healthcare professionals in recognizing their supports, followed by survey of pharmacist’s readiness. Gap analysis was then carried out to identify the additional resources to be provided for conducting the service. About 103 patients were enrolled and eager for drug counseling to be implemented with pharmacist as healthcare counselor. Approximately 46 nurses and 25 active-medical-doctors were participated and giving their support for pharmacist to provide the service; and pharmacists are ready to conduct patient medication counseling with minor adjustment. Gap analysis concluded that additional resources should be furnished, e.g. information module consist of standard information needed by the patient, standard operational procedure, and updated literatures. Due to lack of pharmacist, counseling service only be delivered to patient who has a complex antibiotic regimen therapy. Our data showed that patient medication counseling service in this center could be quickly implemented.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-12-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1226
10.14499/indonesianjpharm28iss4pp232
Indonesian Journal of Pharmacy; Vol 28 No 4, 2017; 232
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1226/856
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1601
2021-01-07T01:00:39Z
3:ART
FTIR Method for Peptide Content Estimation and Degradation Kinetic Study of Canarium Nut Protein
Oktaviary, Rozana
Nugrahani, Ilma
Surantaatmadja, Slamet Ibrahim
Kartawinata, Tutus Gusdinar
Apsari, Cintya Nurul
Canarium nut; peptide; degradation; FTIR; kinetic
Quantitative analysis of bioactive peptide mostly conducted by measuring the activity. While the determination of peptide content in natural sources has been conducted using various instruments, vibrational spectroscopy remains underutilized. Here, we attempted new developed method in peptide quantification and degradation kinetic analysis using Fourier-Transform Infrared Spectroscopy. Bovine Serum Albumin was used as standard protein in method development and validation. Peptide content was estimated by converting peak area to concentration. The method was used to estimate peptide content in Canarium nut protein and its hydrolysates, which potentially hold biological activity. Kinetic study was conducted with microwave as an accelerator for hydrolysis, an apparatus rarely used in peptide study. Amide I band on wavenumber range of 1724.05-1619.91 cm-1 was selected for analysis, considering its selectivity and linearity. The method also met other validation requirement, including accuracy and precision. When applied in quantitative analysis, the method was able to calculate peptide content decrease in Canarium nut protein after hydrolysis using papain (38.24%), pepsin (33.67%) and alkaline reagent (28.53%). In kinetic study, microwave-assisted peptide degradation exhibited logarithmic profile with the equation of y=-0.148ln(x)+0.9591 and R² value of 0.963. Based on these results, FTIR is useful in estimating peptide content and in analyzing degradation kinetic profile.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-07-10
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1601
10.14499/indonesianjpharm31iss2pp78
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 78
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1601/949
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/662
2020-01-27T02:53:58Z
3:ART
SELAGINELLA ACTIVE FRACTIONS INDUCE APOPTOSIS ON T47D BREAST CANCER CELL
Handayani, Sri; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Risdian, Chandra; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Meiyanto, Edy; Faculty of Pharmacy, Universitas Gadjah
Mada, Indonesia. Skip Utara 55281
Yogyakarta
Udin, Zalinar; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Andriyani, Rina; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Angelina, Marissa; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Apoptosis is an important target on anticancer mechanism. The purpose of this research is to investigate apoptosis induction of Selaginella plana Hieron active fractions on T47D cells. Absolute ethanol was used to extract Selaginella plana powders. Ethanolic extract was dilluted by methanol:water (4:1) and then fractionated by hexane (S_Hex), methylene chloride (S_MTC), ethyl acetate (S_EA), and buthanol (S_BuOH). The proliferation of T47D cell line was detected by SRB (Sulforhodamine B) assay which was measured at a wavelength of 515nm. Flowcytometry analysis to determine apoptosis was examined by Propidium Iodide (PI) and Annexin V assay using T47D breast cancer cell line. The result showed that the IC50 value of S_Hex, S_MTC, S_EA, and S_BuOH on T47D cells were 107 µg/mL, 4 µg/mL, 6 µg/mL, and 17 µg/mL respectively. The active fractions (S_MTC and S_EA) at its IC50 concentration significantly (P<0.05) increased the total number of early apoptotic cells in the T47D cells 3.39% and 4.1% respectively compared to that of control (1.95%). Based on the result, methylene chloride and ethyl acetate fraction of Selaginella plana induced apoptosis on T47D cell.Keywords: apoptosis, breast cancer, Selaginella
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/662
10.14499/indonesianjpharm23iss1pp48-53
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 48-53
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/662/528
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1378
2019-10-03T03:16:50Z
3:ART
Synthesis and Molecular Docking Studies of N’-benzoylsalicylhydrazide derivatives as antituberculosis through InHA enzym inhibition
Santosa, Harry; University of Surabaya
Putra, Galih Satrio; University of Surabaya
Yuniarta, Tegar Achsendo; University of Surabaya
Budiati, Tutuk; Airlangga University
Antituberculosis, Benzoylsalicylhydrazide, Methyl Salicylate, InHA, Microwave synthesis, Molecular docking
The specific aims of this study is to synthesize and to study the possible mechanism of N’-benzoylsalicylhydrazide derivatives as an antituberculosis agent through InhA (Enoyl acyl carrier protein reductase) inhibition using in silico method. Five analogues of N’-benzoylsalicylhydrazide were synthesized using microwave irradiation from methyl salicylate as starting material, which yielded 80-90% product on average. This indicates a considerable improvement in terms of effectivity and efficiency, compared to the more conventional method using reflux condition. Character-ization of the compounds were subsequently carried out by UV, FTIR, 1H-NMR, 13C-NMR spectroscopy, which confirmed that the compounds had been successfully synthesized. Ultimately, molecular docking was performed using Molegro Virtual Docker (MVD) on the active site of InhA enzyme to predict the activity of the compounds. The results showed that all compounds performed comparatively well against N-(4-Methylbenzoyl)-4-benzylpiperidine as the native ligand and also yielded lower docking score than isoniazide (INH). From this study it can be concluded that N’-benzoylsalicylhydrazide derivatives could be synthesized using microwave irradiation with good product yield and all of the synthesized analogues are suggested to possess antituberculosis activity via InhA enzyme inhibition. In vitro activity will have to be determined in the future to validate whether N’-benzoylsalicylhydrazide derivatives perform well as a potential antituberculosis agent.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-11-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1378
10.14499/indonesianjpharm29iss4pp198
Indonesian Journal of Pharmacy; Vol 29 No 4, 2018; 198
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1378/884
Copyright (c) 2018 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1181
2017-08-30T06:55:29Z
3:ART
Estimation of total phenolic, total flavonoid content and evaluation of anti-inflammatory and antioxidant activity of Ixora coccinea Linn. stems
R, Surana A; Department of Pharmacognosy, S.M.B.T. College of Pharmacy
Nandi-Hills, Dhamangaon, Tal-Igatpuri, Dist-Nashik, M.S., India-422403
D, Wagh R; Department of Pharmacognosy, S.M.B.T. College of Pharmacy
Nandi-Hills, Dhamangaon, Tal-Igatpuri, Dist-Nashik, M.S., India-422403
Anti-inflammatory activity; Antioxidant activity; Carrageenan-induced paw edema; HRBC; Ixora coccinea
Ixora coccinea Linn. (Rubiaceae) has mentioned in Ayurveda as Paranti and traditionally stems used in inflammatory diseases like sprains, eczema, contusions and boils. Present study deals with evaluation of anti-inflammatory and antioxidant activity of extracts of I.coccinea stem. Anti-inflammatory activity was studied in vivo by carrageenan-induced paw edema in rat and in vitro by human red blood cell membrane stabilization method. Total tannin and flavonoid content of extracts was determined by using the Folin- Ciocalteu method and aluminum chloride method, respectively. Antioxidant activity was evaluated by in vitro assay involving nitric oxide scavenging, hydrogen peroxide scavenging, 2,2- diphenylpicrylhydrazyl (DPPH) radical scavenging, and ion chelating activity. Chloroform extract showed significant reduction in carrageenan induced rat paw edema (p<0.05) and protection of HRBC in hypotonic solution. Methanol extract contain more total tannin and flavonoid content as compared with petroleum ether and chloroform extract. All extracts showed concentration dependant free radical scavenging activity. Methanol extract and chloroform extract have shown better antioxidant activity and due to this antioxidant nature might be responsible for its anti-inflammatory activity. These activity supports to use of I.coccinea extract in traditional used in treatment of various inflammatory disaeses.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-08-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1181
10.14499/indonesianjpharm28iss2pp99
Indonesian Journal of Pharmacy; Vol 28 No 2, 2017; 99
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1181/812
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1493
2021-01-07T01:00:39Z
3:ART
THE EFFECT OF GIVING DELTAMETHRIN AND CYPERMETHRIN INSECTICIDES ON THE LEVEL OF INTRATESTICULAR TESTOSTERONE IN MALE WISTAR STRAIN RATS
Triutomo, Devyanto Hadi; Faculty of Pharmacy UGM
Puspitasari, Ika; Faculty of Pharmacy, Universitas Gadjah Mada
Susidarti, Ratna Asmah; Faculty of Pharmacy, UGM
cypermethrin, deltamethrin, intratesticular testosterone
The use of deltamethrin and cypermethrin insecticides to control pests has been implemented in Indonesia. Irregular and excessive use of insecticides can have toxic effects on the male reproduction system. Deltamethrin and cypermethrin insecticides can inhibit androgen biosynthesis and disrupt the growth of sperm. Therefore, this research is conducted to find out the effect of deltamethrin and cypermethrin insecticides toward the level of intratesticular testosterone in male Wistar strain rats. This research is conducted in vivo using male Wistar strain rats. Nine rats are divided into three groups of treatment, namely control group, deltamethrin group with 0.26 mg/kg of weight, and cypermethrin group with 0.26 mg/kg of weight. Treatment is conducted every day for 15 days orally, and then the rats are dissected to take their testicles. Testicles are chopped and their intratesticular homogenates are taken by adding the medium of DMEM and collagenase (0.25 mg/mL). The measurement of the level of testosterone is conducted by using Electrochemiluminescence immunoassay (ECLIA) method. Data on the change of the increase of body weight, organ weight, and the level of testosterone is analyzed statistically using the one-way ANOVA test. The research result shows that giving 0.26 mg/kg of weight of deltamethrin and 0.26 mg/kg of weight of cypermethrin does not affect the increase in body weight, relative weight of the reproductive organ, and the level of intratesticular testosterone of male Wistar strain rats.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-08-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1493
10.14499/indonesianjpharm31iss2pp116
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 116
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1493/947
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/673
2020-01-27T02:51:49Z
3:ART
PASSIVE AND IONTOPHORETIC PERMEATION OF CAPTOPRIL GEL: AN IN VITRO AND IN VIVO STUDY
Jain, Ashish; Bansal College of Pharmacy, Kokta, Anand Nagar, Bhopal-462021, India.
Nayak, Satish; Bansal College of Pharmacy, Kokta, Anand Nagar, Bhopal-462021, India.
Soni, Vandana; Department of Pharmaceutical Sciences, Dr H.S.Gour Vishwavidyalaya, Sagar 470003, M.P. India
The Objective of this work was to formulate and evaluate captopril gel to assess its suitability for transdermal delivery by passive and iontophoresis. A polymer gel was prepared using hydroxypropyl methyl cellulose and in vitro skin permeability was assessed in full thickness skin of rabbits and pigs. For in vivo studies New Zealand rabbits were used. In vitro passive permeation was carried out in Franz diffusion cell but for iontophoresis, diffusion cell was modified according to Glikfield design. Iontophoresis was performed at a current density of 0.5 mA/cm2via silver /silver chloride electrodes with passive controls but for in vivo study current density wasreduced to 0.1 mA/cm2. Blood samples were analyzed for drug content by HPLC. Results of the in vitro study indicated that iontophoresis considerably increased the permeation rate of captopril compared to passive controls in both the skin types (P<0.01). The plasma concentration of captopril was significantly higher (P<0.001) than that obtained in the passive controls. Results showed that the target permeation rates for captopril could be achieved with the aid of iontophoresis by increasing the area in an appreciable range.Key words: Captopril, iontophoresis, transdermal, Rabbit, Pigskin, in vitro, in vivo.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/673
10.14499/indonesianjpharm23iss2pp121-129
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 121-129
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/673/539
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1063
2017-09-11T06:07:08Z
3:ART
SYNTHESIS AND SCREENING OF SOME AZOMETHINES BEARING METHYL SALICYLATE MOIETY FOR ANTIBACTERIAL ACTIVITIES
Baluja, Shipra; Department of Chemistry, Saurashtra University, Rajkot-360005, India
Chanda, Sumitra
In this study, the series of novel azomethines were synthesized from methyl salicylate and their structural determination was done by IR, NMR and mass spectral data. The screening of these synthesized compounds were done against some Gram positive and Gram negative bacteria in DMF and DMSO solvents by agar disc method. It is observed that solvent and compound structure both affect the inhibition. DMF is found to be good solvent in the present study. Amongst all studied compounds, JB-4 showed maximum zone of inhibition against most of the studied bacterial strains while compound JB-2 is least effective. Thus, substitution affects inhibition and methyl salicylate moiety with cinnamaldehyde side chain is most effective. Further, virtual screening of these compounds was done to evaluate molecular properties and bioactive scores of these compounds.Keywords: azomethines, methyl salicylate, antibacterial activity, agar disc method, DMF, DMSO
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-03-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1063
10.14499/indonesianjpharm27iss2pp92
Indonesian Journal of Pharmacy; Vol 27 No 2, 2016; 92
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1063/774
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1519
2019-10-28T01:20:02Z
3:ART
A Study of Psychoactive Medicines and Risk of Falls Among Indonesian Elderly Patients
Rahmawati, Fita; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia 55281
Mustafidah, Nasikhatul; Postgraduate Magister of Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia 55281
Department of Pharmacy RSUD. Dr. Soedono Madiun
Pramantara, I Dewa Putu; Department of Geriatric, RSUP Dr. Sardjito Hospital, Yogyakarta, Indonesia 55281
Wahab, Izyan Abdul; Faculty of Pharmacy, Cyberjaya University College of Medical Sciences, Persiaran Bestari, Cyber 11, 63000 Cyberjaya, Selangor, Malaysia
Psychoactive medicine, Fall risk, Elderly
One of the causes of injury to the elderly is due to falls. Falling can be prevented by identifying and controlling risk factors. One risk factor that can be controlled is the use of fall risk medicines including psychoactive. This study aims to identify the association between the use of psychoactive medicine and its characteristic with the risk of falls among the elderly in Indonesia. The study utilized a case-control study design for a total number of 414 elderly patients, during October until December 2018. Cases were elderly aged 60 years or above with a high risk of falling assessed using the Morse Fall Scale (MFS≥45). Each case was matched with up to two randomly selected controls of the same age who are classified as low to moderate risk of falling (MFS<45). The use of psychoactive medicines was screened from a history of drug use for the past six months. Psychoactive medicine-fall risk associations were estimated via logistic regression. There were 138 cases and 276 controls. The median age of subjects was 66 years old and 54.83% was a woman. Elderly with a high risk of falling had higher psychoactive medicines use when compare with controls (31.16 % vs 21.38 %, p< 0.05). After adjusting for potential confounders, the use of psychoactive medicines was significantly associated with higher fall risk in elderly patients (OR 1.79 95% CI 1.10-2.90). Only the duration of psychoactive medication use over 90 days was significantly associated with a high risk of falling (AOR 3.65 95% CI 1.46-9.14). In elderly patients, the continued use of psychoactive medicines increased the risk of fall. Prescribers need to weigh risk and benefit from the use of psychoactive medicines in the elderly to prevent future fall.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Faculty of Pharmacy Universitas Gadjah Mada
2019-07-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1519
10.14499/indonesianjpharm30iss3pp233
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 233
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1519/907
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/413
2017-09-20T03:27:59Z
3:ART
Synthesis of Tetrahydro Pentagavunon-0
Ritmaleni, Ritmaleni; Faculty of Pharmacy, Universitas Gadjah Mada. Yogyakarta, Indonesia.
Simbara, Ari; Faculty of Pharmacy, Universitas Ahmad Dahlan. Yogyakarta, Indonesia.
Curcumin, tetrahydrocurcumin, and monoketone curcumin analogue, pentagamavunone-0 (PGV-0), have been investigated as antioxidant, antiinflammatory and anticancer. This research was aimed to synthesise the Tetrahydropentagamavunone-0 (THPGV-0) compound which assumed as an active metabolite of PGV-0. The hydrogenation reaction was applied to the synthesis of THPGV-0 from PGV-0 using Pd/C 10 % as catalyst at room temperature. The structure elucidation was analysed by using spectroscophy method. The synthetic result showed that THPGV-0 asa white crystalline powder in 25 % with melting point about 122-123 oC.Key words: pentagamavunone-0, Tetrahydropentagamavunone-0, hydrogenation
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2010-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/413
10.14499/indonesianjpharm0iss0pp100-105
Indonesian Journal of Pharmacy; Vol 21 No 2, 2010; 100-105
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/413/293
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1237
2020-03-18T10:32:19Z
3:ART
Analysis of Factors Influencing the Availability of Medicine During JKN Era
Satibi, Satibi; Faculty of Pharmacy, Universitas Gadjah Mada
Ranowijaya, Ranowijaya; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
Aswandi, Aswandi; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
Bermalam, Junagsti; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
Widodo, Gunawan Pamudji; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
JKN; availability of drugs factor; doctors factor; pharmacists factor and patients factor
The availability of medicine is important during National Social Healthy Insurance (JKN) that put into effect in the hospital, one of health service facility. The purpose of this study was to look of the availability of medicines was during JKN era and what factors influence the availability in three Region General Hospitals in Sulawesi. This study was conducted using descriptive method of analytic to get a correlation among doctors, pharmacists and patients as independent variables toward the availability of medicine as dependent variable. The study has been performed in 3 hospitals in Sulawesi. This data obtained were analyzed using statistical model of Linier Regression Analysis with F test or ANOVA test and t test. R was the value of regression which showed the influence of one to another variable. The results showed the level of medicine availability in hospital A was 12.49 months. Factors that influence the availability of medicines were doctors (R=0.778, p= 0.000), pharmacists (R=0.619, p= 0.000) and patients (R=0.653, p= 0.000). All there factor significantly affect the availability of medicines in Hospital A (R=0.675, p=0.000). Hospital B had an excess supply of medicines, with the average of 23.31 month. Factors that influenced the availability of medicines in Hospital B was doctor (R=0.877), pharmacist (R=0.861) and patient (R=0.984) (p=0.000 all). From multiple regression there factors showed a significant correlation to the availability of medicines (R=0.901, p=0.000). Similarity on Hospital A and B, data of the availability of medicine in hospital C was categorized excess as the average of stock was 56.01 month. Factors that influenced the availability of medicines in Hospital C was doctor (R=0.753, p= 0.000), pharmacist (R=0.869, p= 0.000) and patient (R=0.545, p=0.001).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-03-12
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1237
10.14499/indonesianjpharm29iss1pp37
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 37
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1237/837
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/690
2020-01-27T02:53:31Z
3:ART
DEVELOPMENT THE VALIDATION OF INDONESIAN VERSION OF SF-36 QUESTIONNAIRE IN CANCER DISEASE
Perwitasari, Dyah Ariani; Faculty of Pharmacy, Ahmad Dahlan University
Jl. Kapas No.9, Semaki, Yogyakarta, Indonesia
55166.
A Cancer patients need long term therapy in the treatment of their disease. One of the outcome of cancer treatment in patients of quality of life. Because of the limited instrument for measuring cancer patients’ quality of life in Indonesian version, thus this study is aimed to develop the validation of Indonesian version of Short Form-36 questionnaire in cancer patients. The observational study was carried out in this study. Data were collected from cancer patients in the Oncology Department of Sardjito Hospital, Yogyakarta, Indonesia, who were treated with cisplatin at the dosage of ≥ 50mg/m2 as monotherapy or in combinations. The validation process involved known-group validity, discriminant validity, convergent validity and factor analysis. About 203 subjects who were diagnosed with cancer were recruited in this study. Theknown-group validity test showed that there were no significant differences of SF-36 domains among different diagnosa. All of the questions met the criteria of convergent validity except for the questions number 26, 28, 30 and 32. Furthermore, the questions number 2 and 28 did not meet the criteria of discriminant validity. This study presents that many questions are needed to be reconstructed, due to the result of factor analysis which showed that those questions are loaded significantly with other domain’s questions. The development of Indonesian version of SF-36 scales in cancer disease is still less unstatisfactory. According to the result of this study, future study with new construction of questions in this version is still necessary.Key words: validation, SF-36, Indonesia, cancer
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/690
10.14499/indonesianjpharm23iss4pp248-253
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 248-253
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/690/556
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1158
2017-09-11T06:08:04Z
3:ART
FORMULA OPTIMIZATION OF ORALLY DISINTEGRATING TABLET CONTAINING MELOXICAM NANOPARTICLES
Winarti, Lina; Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Jember
Ameliana, Lidya; Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Jember
Nurahmanto, Dwi; Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Jember
orally disintegrating tablet (ODT); meloxicam; milling; nanoparticles
Meloxicam is one of oxicams anti-inflamatory drugs that are effective to relieve toothaches, arthritis, dysmenorrhea, and fever. Meloxicam in this study was milled with High Energy Milling (HEM) method to obtain its nano size and then direct compression method was used to produce Orally Disintegrating Tablet (ODT). ODT is designed to be rapidly dissolved on the tongue within a minute. It can be administered without water or chewing and may improve the bioavailability and effectiveness of the drug, and increase the patient’s adherence. The present study aimed to understand the effects of Ac-Di-Sol and Kollidon CL as superdisintegrants, that were used separately or in combination, on the characteristics of nanoparticles meloxicam ODT. It was also to obtain the best proportion of combination between Ac-Di-Sol and Kollidon CL that can produce the optimum formula of meloxicam ODT. The effects of single or combined superdisintegrants were evaluated using Simplex Lattice Design (SLD). Ac-Di-Sol (X1) and Kollidon CL (X2) were the independent variables, while the dependent variables were friability (Y1), disintegrating time (Y2), wetting time (Y3), and percent meloxicam release after 60 seconds (Y4). Optimization of five nanoparticle meloxicam ODT formulas was conducted using Design Expert 7.1.5. The combination of Ac-Di-Sol 4.05mg (X1) and Kollidon CL 10.95mg (X2) in 150mg nanoparticles meloxicam ODT can produce optimal ODT characteristics. After verification there was no difference between predicted value and observed value with p value > 0.05.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-03-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1158
10.14499/indonesianjpharm28iss1pp53
Indonesian Journal of Pharmacy; Vol 28 No 1, 2017; 53
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1158/804
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1410
2022-10-26T01:05:53Z
3:ART
In Vitro Study: Effect of Cobalt(II) Chloride Against Dengue Virus Type 1 in Vero Cells
Sucipto, Teguh Hari; Institute of Tropical Disease, Universitas Airlangga
Untoro, Yovilianda Maulitiva; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga
Setyawati, Harsasi; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga
Maharani, Anisa; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga
Sholihah, Novia Faridatus; Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga
Churrotin, Siti; Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga
Amarullah, Ilham Harlan; Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga
Soegijanto, Soegeng; Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga
Cobalt(II) chloride, DENV-1, and Antiviral activity.
Dengue virus (DENV) serotypes DENV-1 to DENV-4 are enveloped viruses that belong to the genus Flavivirus of the Flaviviridae. Dengue vaccine or antiviral has not yet been clinically approved for humans, even though there have been great efforts toward this end. Antiviral activity against DENV is needed to develop to be an alternative drug for DENV virus. Cobalt(II) chloride have been used in the treatment and prevention of diseases of humans since ancient times. The aim of this study is to investigate the antiviral effects and Cytotoxicity of Cobalt(II) chloride. This compound was further investigated for its inhibitory effect on the replication of DENV-1 in Vero cells. Antiviral activity and Cytotoxicity measured by WST-1 assay. The IC50 value of the Cobalt(II) chloride for DENV-1 was 0.38 μg/ml. The cytotoxicity of Cobalt(II) chloride to Vero cell suggest that the CC50 value was 2.91 µg/ml The results of this study demonstrate the anti-dengue serotype 1 inhibitory activity of Cobalt(II) chloride was a high toxic compound.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-01-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1410
10.14499/indonesianjpharm30iss4pp316
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 316
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1410/931
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/514
2018-09-05T01:49:55Z
3:ART
EFFECTS OF AVURVEDIC SHODHANA (PROCESSING) ON DRIED TUBEROUS ACONITE (Aconitum napellus Linn.) ROOT
Paul, Arindam; Department of Pharmacology and Pharmacognosy, Babaria Institute of Pharmacy, BITS edu campus, Vadodara-Mumbai NH#8, Varnama, Vadodara - 391240, Gujarat, INDIA
Gajjar, Umang; Department of Pharmacology and Pharmacognosy, Babaria Institute of Pharmacy, BITS edu campus, Vadodara-Mumbai NH#8, Varnama, Vadodara - 391240, Gujarat, INDIA
Donga, Jignasa; Department of Pharmacology and Pharmacognosy, Babaria Institute of Pharmacy, BITS edu campus, Vadodara-Mumbai NH#8, Varnama, Vadodara - 391240, Gujarat, INDIA
Aconite (Aconitum napellus Linn.) commonly known as atis is a poisonous plant used extensively as antihypertensive, antipyretic, analgesic and antirheumatic. Ayurveda recommended the administration of aconite roots only after purification, i.e., boiling roots in cow’s urine (Gomutra). In the present study an attempt was made to compare the pro-arrhythmic and antihypertensive effects of powdered aconite root purified by shodhana process with that of unpurified form of aconite roots in order to provide scientific support of the claim in ayurvedic texts that purification of aconite root by shodhana process retains its antihypertensive activity and is devoid of pro-arrhythmic activity. Aconite root treatment in both forms purified and unpurified) caused significant reduction in BP when compared with diseased control group (P<0.05). The unpurified aconite root group showed significant increase in heart rate, increase in QRS complex time and increase in QT interval, however these parameters were statistically insignificant in purified aconite root treated group. The PRA, SC and BUN levels was significantly decreased in aconite root treatment groups. The probable mechanism of antihypertensive activity of aconite root can be attributed to decrease in plasma renin activity, decrease in oxidative stress and increase in NO levels. Key words: Aconite, shodhana process, antihypertensive, proarrhythmic
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2013-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/514
10.14499/indonesianjpharm24iss1pp40-46
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 40-46
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/514/392
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1357
2018-11-02T06:46:53Z
3:ART
Pharmaceutical applications of Aloe vera
Heng, Huay Chin; Faculty of Pharmacy, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia
Zulfakar, Mohd Hanif; Centre for Drug Delivery Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia
Ng, Pei Yuen; Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia,
50300 Kuala Lumpur, Malaysia
A. vera; anti-diabetic; anti-inflammatory; wound healing; antibacterial
Aloe vera has been used as folk medicine for a host of therapeutic indications of which the inner gel is the component extensively used and studied. Proponents of the use of this plant suggest that it is easily available, economical, and have fewer side effects compared to commercial drug compounds. However, the active constituents and their exact mechanisms have yet to be fully elucidated. This review focuses on the identification of the active constituents and their functional mechanism in the areas of anti-diabetic, anti-inflammatory, wound healing, and antibacterial. Preliminary evidence was found to support the antidiabetic effect which extended to the early stages of the disease with no adverse effects at the dosages used. Although topical application for its anti-inflammatory effect may be delayed and minute, oral administration has shown a significant response. However, too high a dose has been linked with an initial inflammatory reaction. Favorable response to the gel has also been demonstrated to support the use of A. vera in wound-healing and as an antibacterial agent, although limited to simple, uncomplicated wounds. Different active constituents such as acemannan and aloin have been suggested for the effects of the plant and this may be interpreted as synergism among different compounds rather than the action of a single compound. Until robust evidence is available, the plant should only be used as an adjunct to other well-established evidence-based treatment modalities.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Ministry of Education (FRGS/1/2016/SKK09/UKM/02/4)
2018-09-24
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1357
10.14499/indonesianjpharm29iss3pp101
Indonesian Journal of Pharmacy; Vol 29 No 3, 2018; 101
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1357/872
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1026
2017-09-11T04:47:45Z
3:ART
ANTIOXIDANT, ANTIBACTERIAL POTENTIAL AND HPLC ANALYSIS OF Dioscorea alata BULB
-, Md. Anisuzzman -; Science School, Khulna University, Khulna-9208
Zilani, Md. Nazmul Hasan; Science School, Khulna University, Khulna-9208
Khushi, Sharmin Sultana; Fisheries and Marine Resource Technology Discipline, Khulna University, Khulna, 9208
--, Md. Asaduzzman -; Department of Fisheries, Sylhet Agricultural University, Sylhet
-, Md. Golam Hossain; Science School, Khulna University, Khulna-9208
The purpose of the present study was to evaluate antioxidant, antibacterial potential and HPLC analysis of methanol extract of Dioscorea alata bulb. Antioxidant activity investigated by DPPH free radical scavenging assay, the IC50 value of plant extract was24.99µg/mL. Significant absorption of extract (1.317 of 1mg/mL) showed the ferric reducing activity. HPLC analysis indicated the presence of myricetin (471.53mg/100 g of dry extract) in the highest concentration. Extract showed significant zone of inhibition in antibacterial activity test. The presence of myricetin along with other detected polyphenols might contribute to antioxidant and antibacterial activities. Key words: Total Phenolic content, Myricetin, Scavenging activity, Disc diffusion
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1026
10.14499/indonesianjpharm27iss1pp9
Indonesian Journal of Pharmacy; Vol 27 No 1, 2016; 9
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1026/762
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1505
2019-10-10T01:29:33Z
3:ART
In Vitro Study of the Combination of Doxorubicin, Curcuma xanthorrhiza, Brucea javanica, and Ficus septica as a Potential Novel Therapy for Metastatic Breast Cancer
Sutejo, Ika Rahmawati; Biochemistry Department, Faculty of Medicine, University of Jember, Jalan Kalimantan 37 Jember, Indonesia
Cancer Chemoprevention Research Center, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Putri, Herwandhani; Cancer Chemoprevention Research Center, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Handayani, Sri; Cancer Chemoprevention Research Center, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Research Center for Chemistry, Indonesian Institute of Sciences (LIPI), Gedung 452 Kawasan PUSPIPTEK Serpong, Tangerang Selatan, Banten 15314, Indonesia
Jenie, Riris Istighfari; Department of Pharmaceutical Chemistry Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Cancer Chemoprevention Research Center, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta 55281, Indonesia
Meiyanto, Edy; Professor of Pharmaceutical chemistry, Faculty of Pharmacy. Universitas Gadjah Mada
C xanthorrhiza, Bjavanica, F septica, antimetastasis, 4T1 cells
Less optimized therapeutic effects constrain the use of doxorubicin as the main agent of chemotherapy for metastatic breast cancer, resistance and side effects. Therefore we need a combination of more than one chemopreventive agent which has different molecular targets to solve that problem. The aims of this study is to prove the inhibitory effect of ethanolic extract of rhizome of Curcuma xanthorrhiza (ECx), fruit of Brucea javanica (EBj), leave of Ficus septica (EFs) and doxorubicin (Dox) alone and its combination on migration and invasion of a highly metastatic 4T1 breast cancer cell line. Cytotoxic activity of single and combination treatment was evaluated by MTT assay, followed by an experiment of apoptosis induction by using flow cytometry. The inhibitory effect on migration was observed by the scratch wound-healing assay. Furthermore, the observation of the activity of matrix metalloproteinase-9 (MMP-9) was analyzed by gelatin zymography. The results showed that ECx, EBj, EFs, and Dox has cytotoxic activity on 4T1 cells with the value of IC50 respectively 49.7±1.53mg/mL, 59.9±1.79mg/mL, 15.2±2.12mg/mL and 1.2±0.23mM. Furthermore, combination of ECx-EBj-Dox and ECx-EBj-EFs revealed synergistic effect on 4T1 cells and decrease cell viability through the induction of apoptosis and necrosis. Based on wound healing assay, 24 hours incubation of this combination inhibited 4T1 cells migration compared to single treatment. Gelatin zymography analysis showed that this combination also inhibited the activity of MMP-9 greater than a single use. Curcuma xanthorrhiza, Brucea javanica, and Ficus septica may have potential to be developed as a combination with or without doxorubicin for metastatic breast cancer treatment.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1505
10.14499/indonesianjpharm30iss1pp15
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 15
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1505/889
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/661
2020-01-27T02:53:58Z
3:ART
PREDICTORS OF THE USE OF GASTROPROTECTIVE AGENTS IN PATIENTS USING NSAID IN YOGYAKARTA INDONESIA
Indrianto, Ady Bagus; Postgraduate Program, Faculty of Pharmacy,
Universitas Gadjah Mada
Thobari, Jarir At; Department of Pharmacology and Toxicology, Faculty of Medicine, Universitas Gadjah Mada
Nugroho, Agung Endro; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta Indonesia 55281
Non Steroid Anti-Inflammatory (NSAID) is one type of AntiInflammatory drug that is used to treat pain in patients of osteoarthritis (OA) and rheumatoid arthritis (RA). The use of NSAIDs can cause gastrointestinal side effects. To prevent the side effects, NSAIDs are prescribed in combination with gastroprotective agent (GPA). The aim of the study is to determine the factors considered in the GPA prescription by the doctors in patients who have risk factors for gastric disorder due to NSAIDs use. This study was performed using a cross-sectional observational design. The data were collected retrospectively during a period of January to December 2010. The subjects were OA and RA patients considering the inclusion and exclusion criteria. The data included patient characteristics (gender, age, occupation, payment method, the primary diagnosis), prescription of NSAID, prescription of GPA, history of gastrointestinal disease, NSAID prescription with corticosteroids, prescription with antiplatelet. The data were processed descriptively and quantitatively, and analyzed statistically using chi-square and log. regression with 95% Confidence Interval. The results showed that there were correlation between some predictor factors with prescription of gastroprotective agent. These factors were women patient, patient age of ≥ 65 years, oxicam prescribing, diclofenac sodium prescribing, prescribing >1 type of NSAIDs, prescription of NSAID concomitant with corticosteroids, prescription of NSAID concomitant with antiplatelet, patients with history of gastrointestinal, prescribing NSAIDs with duration of ≥ 3 months. Patient with a history of gastrointestinal disease was the most predictor influential factor, with OR (odds ratio) of 3.6 (95% CI: 2.79 - 4.66). Predictor factor of patients with a history of dyspepsia possessed the highest OR {OR=4,29 (CI 95%: 3.23–5.7)}. It means that patients with a history of dyspepsia prescribed NSAIDs would have greater risk of 4.29 times to get GPA prescription than patients without a history of dyspepsia. Prescribing NSAIDs > 1 DDD (defined daily dose) had p value of 0.777, which means there was no relationship between GPA prescription with NSAIDs > 1 DDD prescription.Key words: Gastroprotective Agent, Non-steroid anti-inflammatory, Osteoarthritis, Rheumatoid Arthritis
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/661
10.14499/indonesianjpharm23iss1pp41-47
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 41-47
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/661/527
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
25e18a8be6fe459d98a5eae2ccfee9f3