2024-03-29T01:58:12Z
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/oai
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1378
2019-10-03T03:16:50Z
3:ART
Synthesis and Molecular Docking Studies of N’-benzoylsalicylhydrazide derivatives as antituberculosis through InHA enzym inhibition
Santosa, Harry; University of Surabaya
Putra, Galih Satrio; University of Surabaya
Yuniarta, Tegar Achsendo; University of Surabaya
Budiati, Tutuk; Airlangga University
Antituberculosis, Benzoylsalicylhydrazide, Methyl Salicylate, InHA, Microwave synthesis, Molecular docking
The specific aims of this study is to synthesize and to study the possible mechanism of N’-benzoylsalicylhydrazide derivatives as an antituberculosis agent through InhA (Enoyl acyl carrier protein reductase) inhibition using in silico method. Five analogues of N’-benzoylsalicylhydrazide were synthesized using microwave irradiation from methyl salicylate as starting material, which yielded 80-90% product on average. This indicates a considerable improvement in terms of effectivity and efficiency, compared to the more conventional method using reflux condition. Character-ization of the compounds were subsequently carried out by UV, FTIR, 1H-NMR, 13C-NMR spectroscopy, which confirmed that the compounds had been successfully synthesized. Ultimately, molecular docking was performed using Molegro Virtual Docker (MVD) on the active site of InhA enzyme to predict the activity of the compounds. The results showed that all compounds performed comparatively well against N-(4-Methylbenzoyl)-4-benzylpiperidine as the native ligand and also yielded lower docking score than isoniazide (INH). From this study it can be concluded that N’-benzoylsalicylhydrazide derivatives could be synthesized using microwave irradiation with good product yield and all of the synthesized analogues are suggested to possess antituberculosis activity via InhA enzyme inhibition. In vitro activity will have to be determined in the future to validate whether N’-benzoylsalicylhydrazide derivatives perform well as a potential antituberculosis agent.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-11-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1378
10.14499/indonesianjpharm29iss4pp198
Indonesian Journal of Pharmacy; Vol 29 No 4, 2018; 198
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1378/884
Copyright (c) 2018 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/689
2020-01-27T02:53:31Z
3:ART
PHYTOCHEMICAL STUDIES OF Acacia catechu
Baluja, Shipra; Department of Chemistry, Saurashtra University, Rajkot-360005 (Gujarat), INDIA
Chanda, Sumitra; Department of Biosciences, Saurashtra University, Rajkot-360005 (Gujarat), INDIA.
Solanki, Asif; Department of Biosciences, Saurashtra University, Rajkot-360005 (Gujarat), INDIA.
Kachhadia, Nikunj; Department of Biosciences, Saurashtra University, Rajkot-360005 (Gujarat), INDIA.
Acacia catechu Willd. belongs to the family Mimosaceae. It is well known for the powder of wood known as Kathha” in India, which has wide applications. In the present work, the dry wood powder is extracted in various solvents such as 1,4-dioxane, methanol, dimethyl formamide (DMF), acetone, ethanol and distilled water. The phytochemical and physicochemical properties of these various solvent extracts were studied. The phyto constituents present in the different extracts were saponins, tannins, sugars, terpenes, catechu-tannic acid. Alkaloids were present in organic solvents only. The antibacterial activity of all the extracts in DMSO was studied against five bacterial strains but none of the extracts inhibit any of the studied bacterial strains.Key words: Acacia catechu, 1,4-dioxane, methanol, dimethyl formamide (DMF), acetone, ethanol, distilled water, phytochemical analysis, physicochemical analysis.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/689
10.14499/indonesianjpharm23iss4pp238-247
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 238-247
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/689/555
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1702
2021-02-13T17:05:53Z
3:ART
The Effect of Brazilin from Caesalpinia sappan on Cell Cycle and Modulation and Cell Senescence in T47D cells
Jenie, Riris; 1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
2Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Handayani, Sri; 2Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
3Research Division for Natural Product Technology, Indonesian Institute of Sciences (LIPI), Indonesia
Susidarti, Ratna Asmah; 1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
2Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Meiyanto, Edy; 1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
2Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
brazilin, Caesalpinia sappan, T47D, cell cycle, cell senescence
Ethanolic extract and brazilein-containing fraction of Caesalpinia sappan L., has been reported to inhibit cell proliferation in T47D (ER+ PR+/- cell, Luminal A subtype model). The Luminal A subtype is the most common subtype of breast cancer in Indonesian women. In this study, we explored the activity of the reduced form of brazilein, i.e. brazilin, in T47D cells proliferation and the mechanism that involved. The cytotoxicity activity of brazilin was observed using MTT assay. While the cell cycle modulation analysis was done by using flowcytometry, and the senescence assay was observed using S-A-β-galactosidase assay. The results showed that brazilin inhibited cell growth in a dose-dependent manner with an IC50 value of 50μM (or 14.3μg/mL). That was higher than a brazilein-containing fraction, which was reported previously by our group to have an IC50 value of 68μg/mL against the same cell. Cell cycle analysis showed that cells treated with brazilin were accumulated at the G2/M phase in a dose-dependent manner. Furthermore, cells treated with a combination of brazilin and doxorubicin was accumulated at the G2/M phase and sub G1 phase. Cells accumulation at sub G1 phase indicates that the cells undergo apoptosis. Our data of S-A-β-galactosidase assay showed that cells treated with 1/4IC50, 1/2IC50, and IC50 brazilin had lower senescent cells compared to the untreated cells. The morphology of cells treated with IC50 (50μM) brazilin changed. The cells shape became rounded, cells were shrinkage and detached from the well plate, indicating that cells may undergo apoptosis. These results suggested that brazilin was cytotoxic towards T47D cells and its combination with dox potentially induced apoptosis and decreased cell senescence. The ability of brazilin to decrease cell senescence provides new insight of utilization of C. sappan or its constituents, particularly brazilin, as anti-ageing.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
The Ministry of Research and Technology, Higher Education, Republic of Indonesia
2020-07-10
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1702
10.14499/indonesianjpharm31iss2pp84
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 84
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1702/948
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1107
2017-08-30T06:55:29Z
3:ART
The Effect of Medication Reminder Chart on Level Adherence in diabetes mellitus type 2 patients at RSUD Sleman Yogyakarta
Suffiana, Yunita; Gadjah Mada University
Rahmawati, Fita; Gadjah Mada University
Andayani, Tri Murti; Gadjah Mada University
Compliance, Clinical outcome, MMAS-8, Medication Reminder Chart
Patient adherence is a key factor that determines the outcome of diabetes mellitus therapy. This study was conducted to determine the effect of a medication reminder chart on level adherence and clinical outcome in patients with Type 2 diabetes mellitus. The experimental study used pretest-posttest design with control group was conducted during February until April of 2016 at Sleman Hospital in Yogyakarta. Patients were categorized into two groups of different subjects, who received of medication reminder chart tools were 40 patients (Code A) and the other 40 patients were assigned to control group (Code B). Morisky Medication Adherence Scale of 8-item (MMAS-8) was used to measure adherence. Clinical outcome were measured based on FBG decrease (mg/dl). Two independent sample t-test was using to determine the mean differences of the average score of the adherence level before and after using the medication reminder chart. The results of this study showed that Medication Reminder Chart compared control groups improved adherence (p=0.000) but did not improve clinical outcome (p=0.411). The pharmacist might use The Medication Reminder Chart to improve medication adherence for chronic disease.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-06-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1107
10.14499/indonesianjpharm28iss2pp125
Indonesian Journal of Pharmacy; Vol 28 No 2, 2017; 125
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1107/808
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/513
2018-09-05T01:49:55Z
3:ART
SYNTHESIS AND ANTIMICROBIAL ACTIVITY EVALUATION OF SOME SCHIFF BASES DERIVED FROM 2-AMINOTHIAZOLE DERIVATIVES
Gupta, Rajul; Dept. of Pharmacy, CMJ University, Modrina Mansion, Laitumkhrah, Shillong, Meghalaya-793003, India.
Fuloria, Neeraj Kumar; Dept. of Pharmaceutical Chemistry, Anuradha College of Pharmacy, Chikhli, Dist. Buldana, Maharashtra, 443201, India.
Fuloria, Shivkanya; Dept. of Pharmaceutical Chemistry, Anuradha College of Pharmacy, Chikhli, Dist. Buldana, Maharashtra, 443201, India.
Various substituted acetophenones (1-5) on treatment with iodine and thiourea yielded 2-amino-4-(substituted-phenyl)thiazole (1a-5a), which on further treatment with various substituted aldehydes to get N-(substitutedbenzylidene)-4(substitutedphenyl)thiazol-2-amine (1b-5b). All the synthesized compounds were characterized by their respective FTIR, H NMR and Mass data. Synthesized compounds (1b-5b) were subjected to investigation for their antibacterial and antifungal studies against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Asperigillus flavus and Asperigillus fumigatus by disk diffusion method. Compound 5b was found to be most effective with largest zone of inhibition. Key words: Thiazole, Acetophenones Antibacterial, Antifungal, Substituted Aldehydes.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-09-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
text/html
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/513
10.14499/indonesianjpharm24iss1pp35-39
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 35-39
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/513/391
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/513/912
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1494
2019-10-10T01:30:23Z
3:ART
Pharmacokinetics Interaction and Biodistribution of 5 Fluorouracil with Radiopharmaceuticals 99mTc Glutathione for Cancer Diagnostic in Mice Cancer Model
Kurniawan, Ahmad; National Nuclear Energy Agency
Ambar Wibawa, Teguh Hafidz; National Nuclear Energy Agency
Daruwati, Isti; National Nuclear Energy Agency
Iswahyudi, Iswahyudi; National Nuclear Energy Agency
5 Fluorouracil, 99mTc-Glutathione, Drug-Radiopharmaceutical Interaction, Cancer Diagnostic, Nuclear Medicine
Radiopharmaceutical 99mTc-Glutathione has been developed for cancer diagnostic in nuclear medicine. Interactions between chemotherapy drugs and radiopharmaceuticals can altered radiopharmaceuticals performance. Drug interaction 5-fluorouracil (5-FU) with a radiopharmaceutical 99mTc-Glutathione in mice cancer model has been proven in pharmacokinetics study. The biological half-life distribution of 99mTc-Glutathione for cancer model mice when administrated with 5-FU become longer to 0.340±0.121h if compared with 99mTc-Glutathione. Biological half-life elimination for cancer model mice given with 99mTc-Glutathione is 72.712±2.427h. Administration of 5-FU makes the biological half-life elimination of 99mTc-Glutathione shorter to 17.030±3.459h. Biodistribution study of 5-FU continued with 99mTc-Glutathione for cancer model mice showed higher physiological uptake in the kidney was observed (39.77±2.70%ID/g) for 99mTc-Glutathione has lower uptake on kidney (29.55.3.73 %ID/g) with p<0.05. Based on calculation on cancer model mice with colon cancer compared with muscle, shown target/non-target (T/NT) ratio 2.93 for 5-FU continued with 99mTc-Glutathione has ratio 0.42. Low ratio T/NT may affect to poor organ visualization for cancer diagnosis. Acute toxicity study has shown drugs safety for clinical purpose. The knowledge about chemotherapy drug interaction with a radiopharmaceutical is important to have a correct diagnosis of the patient on clinical application.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
National Nuclear Energy Agency
2019-06-12
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1494
10.14499/indonesianjpharm30iss2pp91-97
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 91-97
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1494/905
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1025
2017-09-11T04:29:11Z
3:ART
PHARMACEUTICAL PROPERTIES OF VENOM TOXINS AND THEIR POTENTIAL IN DRUG DISCOVERY
Kool, Jeroen; Division of BioAnalytical Chemistry, Department of Chemistry and Pharmaceutical Sciences, Faculty of Sciences, VU University Amsterdam, Amsterdam
Traditional pipelines feeding drugs coming to the market are declining. This is one of the reasons why nowadays the previously abandoned natural extract drug discovery programs are slowly coming back. In this scenario, small molecular metabolites from plants and single cell marine or soil organisms are gaining interest in pharmaceutical research again. Animal venoms are another source for finding new biopharmaceutical lead molecules and research interest in discovering bioactive molecules from venoms is rising. Venoms comprise often highly selective and potent bioactive peptides and small proteins for receptors and enzymes that are valid drug targets. This work discusses drug discovery research on bioactive compounds in venoms and gives older and more recent examples of bioactive compounds found in venoms from different animals. Common pharmaceutical targets that different classes of venom toxins interact with and information on developmental stages of several medicinal venom peptides are also discussed. Key words:venom, toxin, drug discovery, peptide, pharmaceutical activity
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1025
10.14499/indonesianjpharm27iss1pp1
Indonesian Journal of Pharmacy; Vol 27 No 1, 2016; 1
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1025/761
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1731
2020-05-30T04:59:18Z
3:ART
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1120
2017-08-28T03:41:06Z
3:ART
NATIONAL HEALTH COVERAGE SYSTEM : PHARMACISTS AND JKN PARTICIPANT SATISFACTION IN PRIMARY HEALTH FACILITIES
Satibi, Satibi; Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara 55281
National Health Insurance is a health protection system for participants to obtain health care benefits and protection to meet basic health needs that organized based on social insurance and equity principles. JKN in cooperation with the primary health facilities to provide comprehensive health services including pharmacy services. Pharmacist responsible for drug availability and affordability that affected by drug procurement process, drug distribution process, and drug cost claiming process.This research’s aim to determine relationship between national health insurances services to pharmacists satisfaction and to determine relationship between pharmacy services to outpatient satisfaction at primary health facilities. Data used in research are primary data that collected through questionnare and interview. The method used is purposive sampling which the data is analyzed using linear and multiple regresion and see significant value (p). The number of respondents was 40 pharmacists and 150 patients in health centers and primary clinics scattered in Sleman, Bantul, and Kota Yogyakarta. The results showed that the process of procurement and distribution of drugs have a relationship with a pharmacist satisfaction while drug costs not process claims with consecutive p 0.010, 0.002, and 0.261. Related to the satisfaction of the outpatient pharmacy services result there is a relationship of drug availability (p = 0.003), service time (p = 0.006), and get drug (p = 0.000) to the satisfaction of outpatients. The conclusion of this study is the process of procurement and distribution of drugs have a relationship with a pharmacist satisfaction. In addition, the availability of pharmacy services such as medication, a drug services, and the provision of drug information also has a significant relationship to the satisfaction of outpatients in health facilities first rate.Keywords: pharmacy services, pharmacists, patients, JKN
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-09-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1120
10.14499/indonesianjpharm27iss4pp231
Indonesian Journal of Pharmacy; Vol 27 No 4, 2016; 231
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1120/798
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/11
2017-06-07T01:41:32Z
3:ART
BIOACTIVE COMPOUNDS IN BENGKOANG (Pachyrhizus erosus) AS ANTIOXIDANT AND TYROSINASE INHIBITING AGENTS
Lukitaningsih, Endang; Dept. of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara, Bulaksumur,
Yogyakarta 55281, Indonesia
In Indonesia, the roots of bengkoang (Phacyrhizus erosus) have been used as the excipient for sun screening and skin whitening paste. Since the active compounds exhibiting skin whitening or sun screening effect have not previously been studied, the aim of this study was to identify compounds with antioxidant and tyrosinase inhibitor activities. Soxhlet extraction was used as the method of isolation with petroleum ether as the solvent and it was followed by fractionation using ethyl acetate to obtain three isoflavonoids (i.e. daidzein (2); daidzein-7-O-ß-glucopyranose (3); 5-hydroxy-daidzein-7-O-ß-glucopyranose (4)), and a new pterocarpan (i. e. 8,9-furanyl-pterocarpan-3-ol (1)) which antioxidant activities (SC50% values) of 2.11; 11.86; 0.69 and 7.86 respectively. All compounds showed tyrosinase inhibiting activities with IC50 values of 4.38; 5.35; 7.49 and 22.20 mM, respectively for compound 4, 2, 1 and 3. These compounds can be used as antioxidant and skin whitening materials.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2014-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/11
10.14499/indonesianjpharm25iss2pp68
Indonesian Journal of Pharmacy; Vol 25 No 2, 2014; 68
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/11/149
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1289
2018-10-23T02:19:20Z
3:ART
In Vitro Evaluation of Anti-Inflammatory and Anti-Diabetic Potential Effects of Euphorbia Tithymaloides Ethanol Extract
Wandita, Theresia Galuh; Departement of Animal Life and Environmental Science, HANKYONG NATIONAL UNIVERSITY, SOUTH KOREA
Joshi, Najuma; Departement of Animal Life and Environmental Science, HANKYONG NATIONAL UNIVERSITY, SOUTH KOREA
dela Cruz, Joseph Flores; Department of Basic Veterinary Sciences
College of Veterinary Medicine
University of the Philippines Los Banos, PHILIPPINES
Hwang, Seong Gu; Departement of Animal Life and Environmental Science, HANKYONG NATIONAL UNIVERSITY, SOUTH KOREA
Hibiscus rosasinensis, glipizide, mucilage, antidiabetic activity, excipients
This research work aims to develop glipizide tablets using Hibiscus rosasinensis leaves mucilage. The mucilage was extracted by using double distilled water and precipitated with ethanol. The precipitated mucilage was dried and grounded into powder. The tablet utilizing the mucilage as excipient was prepared by wet granulation method. The tablets were subjected to various tests. The evaluatory parameters of tablets were found to be within the limits as per United States Pharmacopoeia NF 24/19. FTIR spectrum reveals that there is no incompatibility between the ingredients used. Diabetes was induced in wistar albino rats using streptozotocin and effect of formulation on blood glucose level was determined. It was observed that the formulation could not sustain the release of glipizide. However, the glipizide release was retarded as the amount of mucilage was increased. It was observed that on the completion of antidiabetic study, the formulation could bring the blood glucose level to normal in group rats. However, the blood glucose level was still elevated in group administered with pure gliplizide. It can be concluded that HRM can be used to formulate glipizide tablets. The formulations with HRM shows better hypoglycemic activity and this may be attributed to antidiabetic activity of Hibiscus rosasinensis.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-03-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1289
10.14499/indonesianjpharm29iss1pp1
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 1
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1289/858
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/675
2020-01-27T02:53:16Z
3:ART
ISOLATION AND IDENTIFICATION OF FLAVONOIDS FROM Sesamum indicum
Sharma, Priyanka; Laboratory of Bioactive Compounds and
Algal Biotechnology Department of Botany,University of Rajasthan, Jaipur- India.
302004
Sarin, Renu; Laboratory of Bioactive Compounds and
Algal Biotechnology Department of Botany,University of Rajasthan, Jaipur- India.
302004
Natural substances have long served as sources of therapeutic drugs. Many substances have been derived from traditional medicines. The plants are rich in secondary metabolites. The medicinal properties of these plants have been attributed to the biochemicals present in the plant materials. In addition to their role in human and animal nutrition, knowledge of micronutrients and phytochemical composition is fundamental to the understanding of modes and mechanisms of action of medicinal plants in general. In the present investigation, quercetin and kaempferol have been isolated and identified from stem, leaves and unorganized cultures of Sesamum indicum and maintained by frequent subculturings on Murashige and Skoog’s medium (1962) supplemented with NAA+BAP(5.0+0.5mg/L). The study showed that maximum content of quercetin and kaempferol was observed in 6 weeks old calli and minimum in stem of S. indicum. The structure of the isolated compound was established on the basis of physical, chemical test and spectroscopic evidences.Key words: Flavonoids, quercetin, kaempferol, Sesamum indicum
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/675
10.14499/indonesianjpharm23iss3pp135-140
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 135-140
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/675/541
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1578
2022-10-26T01:05:53Z
3:ART
The effect of Phyllanthus niruri L extracts on human leukemic cell proliferation and apoptosis induction
Puspita, Nanda Ayu; Syiah Kuala University
Alhebshi, Hasen; School of Environtment and Life Sciences, University of Salford, United Kingdom, M54WT
Phyllanthus niruri L; Leukemia; MOLT-4; K562; Anticancer
Objective: To investigate the effect of Phyllanthus niruri Linn (Euphorbiaceae) in the proliferation of human leukemic cells (MOLT-4 and K562).Methods: Phyllanthus niruri L (P.niruri) was macerated by using various solvents to obtain the crude extracts. Cytotoxicity of the extracts against MOLT-4 and K562 cells was tested using MTT assay to find the IC50 value. To analyse cell cycle progression, cellular DNA was measured using propidium iodide (PI) staining. Apoptosis induction was evaluated using Annexin V-FITC and PI staining and analysed using FACSVerse flow cytometry. Finally, the expression of p53 on MOLT-4 and K562 cell lysate was measured by western blotting, to identify the possible mode of action of the anticancer activity.Results: P. niruri crude extracts demonstrated a potential anti-cancer effect towards MOLT-4 cells (IC50 range was 42.21 ± 4.98 to 97.06 ± 18.29 µg/ml). However, against K562 cells, P.niruri extracts exhibited a lower inhibitory potency (the IC50 was 120.19 ± 8.48 to 256.55 ± 26.22 µg/ml). The results showed the selectivity of the toxic effect of the extracts against MOLT-4 and K562. To evaluate the possible mechanism of action the anticancer effect, we evaluated P. niruri extract action in apoptosis induction and p53 expression. The results showed that methanol and hexane extract inhibited MOLT-4 cell progression from G1 to S-phase, indicating G1 cell arrest. Moreover, apoptotic cell population following treatment of MOLT-4 and K562 cells with methanol extract was markedly increased, showing morphological signs of apoptosis including membrane degradation and chromatin condensation. Furthermore, we found that there was an increase in p53 expression following MOLT-4 treatment with methanol extract, suggesting that p53 induction may be involved in cell apoptosis.Conclusions: The results indicated the involvement of p53 pathway in the mechanism of anti-cancer activity exerted by P. niruri extract on MOLT-4 cells. However, for cancer cells lacking P53 expression, such as K562 cells, apoptosis might take place via other pathways.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Syiah Kuala University, Medical Faculty
David Pye, University of Salford
University of Salford, Biomedical Research Centre
KidsCan Charity
2019-12-03
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1578
10.14499/indonesianjpharm30iss4pp241
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 241
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1578/925
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1226
2018-10-23T02:18:57Z
3:ART
Provision of Patient Medication Counseling Service In A Tertiary-Care Eye Hospital In Bandung: A Gap Analysis of Patient’s Need, Standard and Service Provider Resources
Hendrayana, Tomi; Department of Pharmacology-Clinical Pharmacy, School of Pharmacy, Bandung Institute of Technology
Zazuli, Zulfan; Department of Pharmacology-Clinical Pharmacy, School of Pharmacy, Bandung Institute of Technology
Fadhila, Genialita; Department of Pharmacology-Clinical Pharmacy, School of Pharmacy, Bandung Institute of Technology
Cahyono, Nur Sidik; Department of Pharmacology-Clinical Pharmacy, School of Pharmacy, Bandung Institute of Technology
Provision of new service; patient medication counseling service; gap analysis; tertiary-care eye hospital; pharmaceutical care
Many studies showed that counseling can improve patient adherence and reduce medication-related problems leading to reach an optimal outcome therapy. The aims of this study were to identify patient’s need, healthcare professional’s support, pharmacist’s readiness and step to be taken to meet standard for implementing patient medication counseling service. Questionnaires has been given to hospitalized-patient to identify the patient’s need and to healthcare professionals in recognizing their supports, followed by survey of pharmacist’s readiness. Gap analysis was then carried out to identify the additional resources to be provided for conducting the service. About 103 patients were enrolled and eager for drug counseling to be implemented with pharmacist as healthcare counselor. Approximately 46 nurses and 25 active-medical-doctors were participated and giving their support for pharmacist to provide the service; and pharmacists are ready to conduct patient medication counseling with minor adjustment. Gap analysis concluded that additional resources should be furnished, e.g. information module consist of standard information needed by the patient, standard operational procedure, and updated literatures. Due to lack of pharmacist, counseling service only be delivered to patient who has a complex antibiotic regimen therapy. Our data showed that patient medication counseling service in this center could be quickly implemented.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-12-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1226
10.14499/indonesianjpharm28iss4pp232
Indonesian Journal of Pharmacy; Vol 28 No 4, 2017; 232
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1226/856
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/373
2017-09-20T03:27:45Z
3:ART
Synthesis of 4-phenyl-3,4-tetrahydro-indeno [2,1]-pyrimidin-2-one (LR-1)
Ritmaleni, Ritmaleni; Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta
Nurcahyani, Wahyu; Faculty of Pharmacy Universitas Gadjah Mada Yogyakarta
The synthetic compound 4-phenyl-3,4-tetrahydro-indeno[2,1]- pyrimidin-2-one 20a (LR-1) was synthesised using Biginelli reaction method. The reaction involved benzaldehyde 6, 2-indanone 2 and urea 7 in acid condition. This condensation reaction yielded 15 % of the product 20, at 133,4-135,0oC of melting point and 0.15 (Et2O : CHCl3 = 1 : 3) of Rf value.Keywords : benzaldehyde, indenone-2, urea
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2006-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/373
10.14499/indonesianjpharm0iss0pp149-155
Indonesian Journal of Pharmacy; Vol 17 No 3, 2006; 149-155
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/373/254
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1151
2019-10-10T01:29:33Z
3:ART
Paricalcitol For CKD-MBD Associated With Secondary Hyperparathyroidism: A Case Series Focus On TRAP5b, b-ALP, and DKK-1
Suprapti, Budi; Department of Clinical Pharmacy, Faculty of Pharmacy, Universitas Airlangga, Campus C Jl. Mulyorejo, 10115, Surabaya, Indonesia
Hartono, Frenky; Department of Clinical Pharmacy, Faculty of Pharmacy, Universitas Airlangga, Campus C Jl. Mulyorejo, 10115, Surabaya, Indonesia
Iqbal, Muhammad; Department of Clinical Pharmacy, Faculty of Pharmacy, Universitas Airlangga, Campus C Jl. Mulyorejo, 10115, Surabaya, Indonesia
Zuhri, Muhammad Isnaini; Department of Clinical Pharmacy, Faculty of Pharmacy, Universitas Airlangga, Campus C Jl. Mulyorejo, 10115, Surabaya, Indonesia
Aditiawardana, Aditiawardana; Department of Nephrology, Faculty of Medicine, Dr. Soetomo Hospital, Jl. Mayjen Prof. Dr. Moestopo No.6-8, Airlangga, 60286, Surabaya, Indonesia
Chronic kidney disease (CKD) lead to secondary hyperparathyroidism (sHPT) that caused by phosphate retention and hypocalcemia. This condition known as mineral and bone disorder (CKD-MBD). The increase in parathyroid hormone would increase bone turnover that result in an increased risk of bone fractures, and vascular calcification. These will increase the levels of tartrate-resistant acid phosphatase 5b (TRAP5b), and bone-specific alkaline phosphatase (b-ALP), which is a marker of bone turnover, and also dickkopf-related protein 1 (DKK-1), which is an inhibitor of the Wnt pathway. Secondary hyperparathyroidism in CKD also caused by calcitriol deficiency. Paricalcitol is a synthetic calcitrol analogue used to reduce parathyroid hormone (iPTH) with minimal calcemic and phosphatemic activity. Vitamin D receptor activation by paricalcitol will decrease TRAP5b, b-ALP, and DKK-1. In this study we reported 9 cases of CKD-MBD with Hemodialysis (HD) and associated with sHPT. Four of nine cases received 5μg paricalcitol every HD (twice a week) while the others five is not. Level of iPTH, phosphate, calcium, TRAP5b, b-ALP, and DKK-1 were measured before initiation of study and after three months treatment. According to this study, the paricalcol administration suppresses the increase in iPTH level, bone turnover and vascular calcification showed by decreasing or supresses the increase b-ALP, TRAP5b, DKK-1 leves without increasing calcium and phosphate levels.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-03-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1151
10.14499/indonesianjpharm30iss1pp66
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 66
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1151/895
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1181
2017-08-30T06:55:29Z
3:ART
Estimation of total phenolic, total flavonoid content and evaluation of anti-inflammatory and antioxidant activity of Ixora coccinea Linn. stems
R, Surana A; Department of Pharmacognosy, S.M.B.T. College of Pharmacy
Nandi-Hills, Dhamangaon, Tal-Igatpuri, Dist-Nashik, M.S., India-422403
D, Wagh R; Department of Pharmacognosy, S.M.B.T. College of Pharmacy
Nandi-Hills, Dhamangaon, Tal-Igatpuri, Dist-Nashik, M.S., India-422403
Anti-inflammatory activity; Antioxidant activity; Carrageenan-induced paw edema; HRBC; Ixora coccinea
Ixora coccinea Linn. (Rubiaceae) has mentioned in Ayurveda as Paranti and traditionally stems used in inflammatory diseases like sprains, eczema, contusions and boils. Present study deals with evaluation of anti-inflammatory and antioxidant activity of extracts of I.coccinea stem. Anti-inflammatory activity was studied in vivo by carrageenan-induced paw edema in rat and in vitro by human red blood cell membrane stabilization method. Total tannin and flavonoid content of extracts was determined by using the Folin- Ciocalteu method and aluminum chloride method, respectively. Antioxidant activity was evaluated by in vitro assay involving nitric oxide scavenging, hydrogen peroxide scavenging, 2,2- diphenylpicrylhydrazyl (DPPH) radical scavenging, and ion chelating activity. Chloroform extract showed significant reduction in carrageenan induced rat paw edema (p<0.05) and protection of HRBC in hypotonic solution. Methanol extract contain more total tannin and flavonoid content as compared with petroleum ether and chloroform extract. All extracts showed concentration dependant free radical scavenging activity. Methanol extract and chloroform extract have shown better antioxidant activity and due to this antioxidant nature might be responsible for its anti-inflammatory activity. These activity supports to use of I.coccinea extract in traditional used in treatment of various inflammatory disaeses.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-08-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1181
10.14499/indonesianjpharm28iss2pp99
Indonesian Journal of Pharmacy; Vol 28 No 2, 2017; 99
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1181/812
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/508
2020-02-19T02:37:07Z
3:ART
AKTIVITAS PENGANGKAPAN RADIKAL POLIFENOL DALAM DAUN TEH
Rohdiana, Dadan; Tea and Quinine Research Center
Gambung PO BOX 1013 Bandung 40010
Ability of free radical scavengers of various tea extracts (black tea, fermented tea; green tea , unfermented tea; oolong dan pouchong tea semifermented tea) was studied. The scavenging effects on active oxygen decreased in order semifemented tea > unferfmnted tea > fennented lea. Antioxidant activity on tea often might related wthi catechin presence. Catechin activity as antioxidant estabilished. Several epidemilogical and phartnacokgical research shown that catechin had a strongly antioxidan activity. (-)-epigallocatechin gallate (EGCG) and (-)-epicatechin gallate (ECG) are the major catechin of ~ tea This compounds had a strongly scavenging activity on free radical such as superoxide, hydrogen superoxide, DPPH and peroxynitdte. This review aim to exploited~ tea Oyphenols ability as free radicals sw vengers Key word: antioxidant activity, active oxygen, scavenging effect
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/508
10.14499/indonesianjpharm0iss0pp52-58
Indonesian Journal of Pharmacy; Vol 12 No 1, 2001; 52-58
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/508/386
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1451
2019-10-10T01:30:23Z
3:ART
Formulation, Characterization and Stability of Ibuprofen-Loaded Self-Nano Emulsifying Drug Delivery System (SNEDDS)
Syukri, Yandi; Department of Pharmacy, Universitas Islam Indonesia
Fitriani, Hannie; Department of Pharmacy, Universitas Islam Indonesia
Pandapotan, Herianto; Department of Pharmacy, Universitas Islam Indonesia
Nugroho, Bambang Hernawan; Department of Pharmacy, Universitas Islam Indonesia
Ibuprofen, SNEDDS, characterization, stability
Ibuprofen is a poorly water-soluble drug with analgesic, antipyretic and anti-inflammatory effects. Self-Nano Emulsifying Drug Delivery System (SNEDDS) formulation is a solution to improve the solubility and bioavailability of ibuprofen. This research purposed to perform a formulation, characterization, and stability studies of ibuprofen-loaded Self-Nano Emulsifying Drug Delivery System (SNEDDS). Screening of ibuprofen SNEDDS was prepared by ternary diagrams for the chosen co-surfactants, surfactants, and oil. The following was characterizations of droplet size, zeta potential, and clarity. The solubility test for the determination of co-surfactant, surfactant, and oil obtained Propylene glycol monocaprylate (Capryol-90), Polysorbate 20 (Tween 20) and PEG 400. The screening of SNEDDS showed nine formulas (compositions) in the range concentration of Propylene glycol monocaprylate (1-3 mL), Polysorbate 20 (4-8 mL), and PEG 400 (1-3 mL). The composition of Propylene glycol monocaprylate (1-2 mL), Polysorbate 20 (5-8 mL) and PEG 400 (1-3 mL) passed the thermodynamic stability test. The test of robustness to dilution and stability study indicated that the formula with Propylene glycol monocaprylate, Polysorbate 20 and PEG 400 with the ratio of 1: 8: 1 and 1: 7: 2 was more stable. In conclusion, the stable ibuprofen SNEDDS could be prepared with Propylene glycol monocaprylate, Polysorbate 20, and PEG 400.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-05-27
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1451
10.14499/indonesianjpharm30iss2pp105-113
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 105-113
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1451/900
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/855
2017-09-11T04:54:08Z
3:ART
SUN PROTECTOR FACTOR (SPF) IN VITRO AND THE PHYSICAL STABILITY OF O/W CREAM OPTIMAL FORMULA FROM THE PARTITION PRODUCT OF MAHKOTA DEWA LEAVES [Phaleria macrocarpa (Scheff) Boerl]
Zulkarnain, Abdul Karim; Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta
Marchaban, Marchaban; Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta
Wahyuono, Subagus; Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta
Susidarti, Ratna Asmah; Faculty of Pharmacy, Universitas Gadjah Mada Yogyakarta
The leaves Mahkota dewa (Phaleria macrocarpa (Scheff) Boerl) contains a benzophenoic glycoside (Phalerin) which is active for sun screen. Some formula and designed on the Simplex Lattice Design (SLD) method then its physical characteristics were investigated. The physical characteristics will be used to determine the optimum formula. In present study, the optimum formulation was evaluated to determine chemical stability and the in vitro SPF. The doses of partition product of P. macrocarpa was used 6%. The optimal formulation cream was determined by SLD version 9.0.1 with variety of Cetyl alcohol, mineral oil, and Tween 80. The difference of physical characteristics between experimental and software was analyzed using T-test, P= 95 %. The physical stability and in vitro SPF of the optimum formula was observed. he study exhibits that the combination of cetyl alchodol 9,71%, mineral oil 29%, and tween 80 3,29% was the optimum formula of o/w cream with the desirability test 0,924. The T-test result test shows that there is no significant difference (p>0.05) on physical characteristics between experimental and software prediction. During the storage, the viscosity, spread ability, adhesive ability, separation volume ratio of cream was relatively stable however the stability started to decline on the week IV in the extreme temperature. The SPF of the cream of the CFR, CPHR and CBZP partition were 21,32; 33,12 and 42,49, respectively.Keywords: Mahkota dewa, cream, optimum, SPF
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2015-11-13
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/855
10.14499/indonesianjpharm26iss4pp210
Indonesian Journal of Pharmacy; Vol 26 No 4, 2015; 210
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/855/756
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1176
2018-01-10T06:56:14Z
3:ART
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1063
2017-09-11T06:07:08Z
3:ART
SYNTHESIS AND SCREENING OF SOME AZOMETHINES BEARING METHYL SALICYLATE MOIETY FOR ANTIBACTERIAL ACTIVITIES
Baluja, Shipra; Department of Chemistry, Saurashtra University, Rajkot-360005, India
Chanda, Sumitra
In this study, the series of novel azomethines were synthesized from methyl salicylate and their structural determination was done by IR, NMR and mass spectral data. The screening of these synthesized compounds were done against some Gram positive and Gram negative bacteria in DMF and DMSO solvents by agar disc method. It is observed that solvent and compound structure both affect the inhibition. DMF is found to be good solvent in the present study. Amongst all studied compounds, JB-4 showed maximum zone of inhibition against most of the studied bacterial strains while compound JB-2 is least effective. Thus, substitution affects inhibition and methyl salicylate moiety with cinnamaldehyde side chain is most effective. Further, virtual screening of these compounds was done to evaluate molecular properties and bioactive scores of these compounds.Keywords: azomethines, methyl salicylate, antibacterial activity, agar disc method, DMF, DMSO
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-03-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1063
10.14499/indonesianjpharm27iss2pp92
Indonesian Journal of Pharmacy; Vol 27 No 2, 2016; 92
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1063/774
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1237
2020-03-18T10:32:19Z
3:ART
Analysis of Factors Influencing the Availability of Medicine During JKN Era
Satibi, Satibi; Faculty of Pharmacy, Universitas Gadjah Mada
Ranowijaya, Ranowijaya; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
Aswandi, Aswandi; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
Bermalam, Junagsti; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
Widodo, Gunawan Pamudji; Faculty of Pharmacy, Universitas Setya Budi, Nusukan, Banjarsari, Mojosongo, Jebres, Kota Surakarta, 57127 Jawa Tengah
JKN; availability of drugs factor; doctors factor; pharmacists factor and patients factor
The availability of medicine is important during National Social Healthy Insurance (JKN) that put into effect in the hospital, one of health service facility. The purpose of this study was to look of the availability of medicines was during JKN era and what factors influence the availability in three Region General Hospitals in Sulawesi. This study was conducted using descriptive method of analytic to get a correlation among doctors, pharmacists and patients as independent variables toward the availability of medicine as dependent variable. The study has been performed in 3 hospitals in Sulawesi. This data obtained were analyzed using statistical model of Linier Regression Analysis with F test or ANOVA test and t test. R was the value of regression which showed the influence of one to another variable. The results showed the level of medicine availability in hospital A was 12.49 months. Factors that influence the availability of medicines were doctors (R=0.778, p= 0.000), pharmacists (R=0.619, p= 0.000) and patients (R=0.653, p= 0.000). All there factor significantly affect the availability of medicines in Hospital A (R=0.675, p=0.000). Hospital B had an excess supply of medicines, with the average of 23.31 month. Factors that influenced the availability of medicines in Hospital B was doctor (R=0.877), pharmacist (R=0.861) and patient (R=0.984) (p=0.000 all). From multiple regression there factors showed a significant correlation to the availability of medicines (R=0.901, p=0.000). Similarity on Hospital A and B, data of the availability of medicine in hospital C was categorized excess as the average of stock was 56.01 month. Factors that influenced the availability of medicines in Hospital C was doctor (R=0.753, p= 0.000), pharmacist (R=0.869, p= 0.000) and patient (R=0.545, p=0.001).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-03-12
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1237
10.14499/indonesianjpharm29iss1pp37
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 37
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1237/837
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/667
2020-01-27T02:51:49Z
3:ART
THE OPTIMISED CONDITIONS OF INDUCTION OF RECOMBINANT RIP rMJC15310 ACTIVITY ISOLATED FROM Mirabilis jalapa L. LEAVES
Astuti, Puji; Department of Pharmaceutical Biology,
Faculty of Pharmacy Universitas Gadjah Mada,
Sekip Utara, Yogyakarta, 55281.
., Sudjadi; Department of Pharmaceutical Chemistry, Faculty of Pharmacy Universitas Gadjah Mada, Sekip Utara, Yogyakarta, 55281
., Sismindari; Department of Pharmaceutical Chemistry, Faculty of Pharmacy Universitas Gadjah Mada, Sekip Utara, Yogyakarta, 55281
Ribosome Inactivating Proteins (RIPs) are compounds isolated from plants with ability to inhibit protein synthesis. The inhibition of protein synthesis is due to inactivation of ribosomal RNA through a site-specific deadenylation mediated by RNA N-glycosidase. Reportedly, RIPs mainly possess wide range of bioactivity including antiviral activity against plant infections. Other activities of RIP were as abortifacien, antivirus and anticancer. This study was aimed to isolate and characterize the optimum conditions for inducing the expression of recombinant RIPs isolated from the leaves of Mirabilis Jalapa L. We have been successfully isolated several RIPs and engineered these proteins to be expressed in E. coli. These recombinant proteins were obtained by screening cDNA library originated from the mRNA of Mirabilis jalapa L leaves, and inserted into pUC19 carrying lacZ gene. The presence of recombinant plasmid was tested by using α-complementation assay. Many RIPs have been isolated from plants and these proteins express enzymatic activity by cutting supercoiled double stranded DNA. One RIP namely rMJC15310 was obtained from this study and the proteins having ~ 8kb in size, cut the supercoiled DNA into linear form at the concentration as low as 5 µg. The ability to cut supercoiled DNA increased on inducing its expression with 0.4% IPTG.Key words: Ribosome Inactivating Proteins (RIP), IPTG, Mirabilis jalapa L., recombinant protein
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/667
10.14499/indonesianjpharm23iss2pp93-98
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 93-98
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/667/533
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1514
2019-10-28T01:20:02Z
3:ART
Naringenin-Loaded TPGS Polymeric Nanosuspension: In-Vitro and In-Vivo Anti-Inflammatory Activity
Rajamani, Sumathi; assistant professor
Sengodan, Tamizharasi; assistant professor
Thangavelu, Sivakumar; professor
Shanmukhan, Nikhitha K
Radhakrishnan, Arun; Lecturer
Dep. of pharmaceutics, JSS College of pharmcy, Ooty, JSS Academy of higher education and research
Carrageenan, oxidative stress, anti-inflammatory, Naringenin
Naringenin, (NAR) from Citrus grandis(L.) Osbeck, family Rutaceae, exhibit extensive pharmacological action, lacks significance in application due to low aqueous solubility approximately 0.214 mg/ml, which results in low bioavailability of 5.8%. Nanosuspension of NAR (NARNS) was prepared in our previous studies using high pressure homogenization adding various polymers. All these formulations were characterized and as a continuation of our work formulations was further evaluated for their anti-inflammatory activity by in-vitro and in-vivo methods. Denaturation of protein method and membrane stabilization methods were chosen for in-vitro evaluation. In-vivo studies performed were acute inflammatory studies (carrageenan-induced paw oedema) and chronic inflammatory studies (cotton pellet granuloma) on Wistar albino rats. The studies demonstrated that the NAR and NARNS at a dose of 50 mg/kg P.O. have a potent activity compared to the standard drug diclofenac. The percentage protection against inflammation exhibited by NARNS was highly significant compared to NAR.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-09-24
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1514
10.14499/indonesianjpharm30iss3pp225
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 225
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1514/919
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1158
2017-09-11T06:08:04Z
3:ART
FORMULA OPTIMIZATION OF ORALLY DISINTEGRATING TABLET CONTAINING MELOXICAM NANOPARTICLES
Winarti, Lina; Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Jember
Ameliana, Lidya; Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Jember
Nurahmanto, Dwi; Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Jember
orally disintegrating tablet (ODT); meloxicam; milling; nanoparticles
Meloxicam is one of oxicams anti-inflamatory drugs that are effective to relieve toothaches, arthritis, dysmenorrhea, and fever. Meloxicam in this study was milled with High Energy Milling (HEM) method to obtain its nano size and then direct compression method was used to produce Orally Disintegrating Tablet (ODT). ODT is designed to be rapidly dissolved on the tongue within a minute. It can be administered without water or chewing and may improve the bioavailability and effectiveness of the drug, and increase the patient’s adherence. The present study aimed to understand the effects of Ac-Di-Sol and Kollidon CL as superdisintegrants, that were used separately or in combination, on the characteristics of nanoparticles meloxicam ODT. It was also to obtain the best proportion of combination between Ac-Di-Sol and Kollidon CL that can produce the optimum formula of meloxicam ODT. The effects of single or combined superdisintegrants were evaluated using Simplex Lattice Design (SLD). Ac-Di-Sol (X1) and Kollidon CL (X2) were the independent variables, while the dependent variables were friability (Y1), disintegrating time (Y2), wetting time (Y3), and percent meloxicam release after 60 seconds (Y4). Optimization of five nanoparticle meloxicam ODT formulas was conducted using Design Expert 7.1.5. The combination of Ac-Di-Sol 4.05mg (X1) and Kollidon CL 10.95mg (X2) in 150mg nanoparticles meloxicam ODT can produce optimal ODT characteristics. After verification there was no difference between predicted value and observed value with p value > 0.05.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-03-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1158
10.14499/indonesianjpharm28iss1pp53
Indonesian Journal of Pharmacy; Vol 28 No 1, 2017; 53
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1158/804
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/814
2017-09-12T07:00:50Z
3:ART
NARINGENIN-LOADED CHITOSAN NANOPARTICLES FORMULATION, AND ITS IN VITRO EVALUATION AGAINST T47D BREAST CANCER CELL LINE
Winarti, Lina; Dept of Pharmaceutics, Faculty of Pharmacy,
University of Jember,
Jember 68121, Indonesia
Ruma Kumala Sari, Lusia Oktora; Dept of Pharmaceutics, Faculty of Pharmacy,
University of Jember,
Jember 68121, Indonesia
Nugroho, Agung Endro; Dept of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas
Gadjah Mada, Yogyakarta 55281, Indonesia.
Naringenin (NAR), a natural flavonoid aglycone of naringin has been extensively investigated for its pharmacological activities, including anti-tumor effects. However, its poor bioavailability has been identified as the single most important challenge in oral drug delivery. Based in this condition, it is used nanoencapsulation to increase the effectiveness of NAR as anti-cancer. The objectives of this research are to develop the formulation of NAR-loaded nanoparticles (NARNPs) as well as to evaluate its potential as anti-cancer against T47D breast cancer cells line. NARNPs is prepared through the method of ionic gelation, meanwhile its characteristic is evaluated through photon correlation spectroscopy (PCS), transmission electron microscopy (TEM), fourier transform infra-red spectroscopy (FTIR), and different scanning calorimeter (DSC). The result of MTT test and cellular uptake indicate that NARNPs increase citotoxicity and internalization of NAR to the cells compared to the free NAR. The result of qualitative apoptosis study using fluorescence microscope indicates that both free NAR and NARNPs are able to induce apoptosis. It can be conclude that Chitosan nanoparticles–TPP conjugates have the capability to encapsulate naringenin hence increase the cellular uptake and cytotoxcicity of naringenin against T47D cell line. NARNPs also can induce the apoptosis effect.Keywords: NAR, Chitosan (CS), ionic gelation, nanoparticles
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2015-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/814
10.14499/indonesianjpharm25iss3pp147
Indonesian Journal of Pharmacy; Vol 26 No 3, 2015; 147
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/814/747
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1357
2018-11-02T06:46:53Z
3:ART
Pharmaceutical applications of Aloe vera
Heng, Huay Chin; Faculty of Pharmacy, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia
Zulfakar, Mohd Hanif; Centre for Drug Delivery Research, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia
Ng, Pei Yuen; Drug and Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia,
50300 Kuala Lumpur, Malaysia
A. vera; anti-diabetic; anti-inflammatory; wound healing; antibacterial
Aloe vera has been used as folk medicine for a host of therapeutic indications of which the inner gel is the component extensively used and studied. Proponents of the use of this plant suggest that it is easily available, economical, and have fewer side effects compared to commercial drug compounds. However, the active constituents and their exact mechanisms have yet to be fully elucidated. This review focuses on the identification of the active constituents and their functional mechanism in the areas of anti-diabetic, anti-inflammatory, wound healing, and antibacterial. Preliminary evidence was found to support the antidiabetic effect which extended to the early stages of the disease with no adverse effects at the dosages used. Although topical application for its anti-inflammatory effect may be delayed and minute, oral administration has shown a significant response. However, too high a dose has been linked with an initial inflammatory reaction. Favorable response to the gel has also been demonstrated to support the use of A. vera in wound-healing and as an antibacterial agent, although limited to simple, uncomplicated wounds. Different active constituents such as acemannan and aloin have been suggested for the effects of the plant and this may be interpreted as synergism among different compounds rather than the action of a single compound. Until robust evidence is available, the plant should only be used as an adjunct to other well-established evidence-based treatment modalities.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Ministry of Education (FRGS/1/2016/SKK09/UKM/02/4)
2018-09-24
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1357
10.14499/indonesianjpharm29iss3pp101
Indonesian Journal of Pharmacy; Vol 29 No 3, 2018; 101
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1357/872
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/684
2020-01-27T02:53:31Z
3:ART
PHYSICAL PROPERTIES OF WOUND HEALING GEL OF ETHANOLIC EXTRACT OF BINAHONG (Anredera cordifolia (Ten) Steenis) DURING STORAGE.
Yuliani, Sri Hartati; Faculty of Pharmacy, Universitas Gadjah Mada,
Skip Utara, Yogyakarta 55281 Indonesia
Faculty of Pharmacy, Universitas Sanata Dharma, Paingan, Maguwoharjo, Depok, Yogyakarta 55284 Indonesia
Fudholi, Achmad; Faculty of Pharmacy, Universitas Gadjah Mada,
Skip Utara, Yogyakarta 55281 Indonesia
Pramono, Suwijiyo; Faculty of Pharmacy, Universitas Gadjah Mada,
Skip Utara, Yogyakarta 55281 Indonesia
., Marchaban; Faculty of Pharmacy, Universitas Gadjah Mada,
Skip Utara, Yogyakarta 55281 Indonesia
Binahong (Anredera cordifolia (Ten) Steenis) has been used as wound healing in traditional Indonesian medicine. The developing of the dosage forms using the formulation technology approach has been done. The aim of this study was to examine the physical properties of the wound healing gel of ethanolic extract of binahong during storage. The factorial design method 3 factors and 2 levels was employed to achieve this study. The three factors used in this study were Carbopol, CMC-Na and Caalginate with low and high level for each factor. The physical properties of the wound healing gel of ethanolic extract of binahong was evaluated on day 1, 30, 60 and 90 for viscosity and bioadhesion study. The result showed that CMC-Na and Caalginate increased the alteration of physical properties of wound healing gel of ethanolic extract of binahong during storage. The carbopol maintained the physical properties of the gel during storage.Key words: bioadhesion, binahong, viscosity, wound healing gel.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/684
10.14499/indonesianjpharm23iss4pp203-208
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 203-208
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/684/550
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1652
2021-01-06T13:13:53Z
3:ART
Curcumin Analogs Induce Apoptosis and G2/M Arrest In 4T1 Murine Triple-Negative Breast Cancer Cells
Murwanti, Retno; Universitas Gadjah Mada
Rahmadani, Azmi; Universitas Gadjah Mada
Ritmaleni, Ritmaleni; Universitas Gadjah Mada
Hermawan, Adam; Universitas Gadjah Mada
Sudarmanto, Bambang Sulistiyo Ari; Universitas Gadjah Mada
Apoptosis; Cell cycle arrest; PGV-0; PGV-1; TNBC
Chemotherapy is the first-line treatment for triple-negative breast cancer (TNBC), yet toxicity and resistance effects have been the current problems. Curcumin,a natural compound, has been reported to exert anti-proliferative effects on various cancer cells, including breast carcinoma cells. However, the β-diketone moiety influences the stability of curcumin. Curcumin analogs, pentagamavunon-0 (PGV-0), and pentagamavunon-1 (PGV-1) were synthesized to improve the stability and activity of curcumin by modified the β-diketone moiety into mono-ketone pentanone. In this study, we evaluated the cytotoxicity, inhibition of cell cycle progression, and induction of apoptosis of curcumin and its analogs (PGV-0 and PGV-1) in murine triple-negative breast cancer 4T1 cell line. The cytotoxic evaluation was done by MTT assay, while apoptosis induction and cell cycle evaluation was performed by annexin V staining and detected by flow cytometry. Curcumin and its analogs, PGV-0, and PGV-1, significantly inhibit the viability of 4T1 breast cancer cells with an IC50 value of 34.34µg/mL, 13.76µg/mL and 38.21μg/mL, respectively. Apoptosis analysis with a dose of 10µg/mL and 15µg/mL in 4T1 breast cancer cells showed that curcumin and its analogs effectively induce apoptotic in a dose-dependent manner. In cell cycle analysis using a dose of 15µg/mL, curcumin inhibited the cell cycle progression in the S phase, whereas PGV-0 and PGV-1 inhibited the cell cycle in the G2/M phase. It could be concluded that curcumin analogs, PGV-0 and PGV-1, have higher potential to be developed as anti-cancer agents by inducing cell cycle arrest and apoptosis in triple-negative breast cancer.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Ministry of Research, Technology and Higher Education of the Republic of Indonesia
2020-04-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1652
10.14499/indonesianjpharm31iss1pp11
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 11
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1652/938
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1233
2018-01-09T08:39:15Z
3:ART
FORMULATION OF FLUCONAZOLE AS TOPICAL ANTIFUNGAL GELS BY MICROSPONGE BASED DELIVERY SYSTEMS
Mohan, Swamykannu Dinesh; Jawaharlal Nehru University- Hyderabad
Department of Pharmaceutics, Vishnu Institute of Pharmaceutical Education and Research, Narsapur, Hyderabad
Gupta, Vangadari Rama Mohan; Department of Pharmaceutics, Pullareddy Institute of Pharmacy, Annaram, Hyderabad, 502313. India
Fluconazole, Microsponge, Ethyl cellulose, Scanning electron microscopy and Zero order kinetics
The purpose of present work was to formulate Fluconazole loaded microsponge-based topical delivery system for modified release. Microsponges with varied drug–polymer ratios were prepared by emulsion solvent diffusion technique using Ethyl cellulose as release retard material. Prepared microsponges were studied for particle size and physical characterization. Scanning electron microscopy (SEM) images showed the microsponges porous and spherical in shape. The microsponges were then incorporated in carbopol gel and evaluated for pH, viscosity, spreadability, drug content, in-vitro release. The In vitro drug release showed that microsponges with 1:1.5 drug–polymer ratios (F3) were more efficient to give sustained release of 74.2% at the end of 8 hr. All the microsponge gel formulations (i.e.F1- F10) showed better results like pH between 6.5-7.0, viscosity between 25,030-47,390 cps, spreadability 2-4 cm/s and drug content of 76.20±0.02% to 96.41±0.01%. Hence, the fabricated microsponge based formulation of Fluconazole would be anticipation and promising substitute to conventional therapy of skin infections.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-08-08
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1233
10.14499/indonesianjpharm28iss3pp158
Indonesian Journal of Pharmacy; Vol 28 No 3, 2017; 158
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1233/833
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/442
2020-02-19T02:37:07Z
3:ART
A COMPARATIVE BIOAVAILABILITY OF FUROSEMIDE IN SOLID DISPERSIONS FORMS
Syukri, Yandi; Departement of Pharmacy
Faculty of Science and Mathematic
Islamic Indonesa University
Hakim, Lukman; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
Yuwono, Tedjo; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
Furosemide is a poorly soluble diuretic drug, the solubility of which can be enhanced by solid dispersion with polyvinylpirolidon (PVP). The solid dispersion system was prepared by a solvent method in various ratios of 1 : 5 and 1 : 7 of the drug and PVP, respectively, in order to improve furosemide bioavailability. The bioavailability of furosemide - PVP solid dispersion was compared with pure furosemide (control) and Lasix (reference). The study was done in a cross over design with a single-dose peroral that administered to the white male rabbits (n = 6). Furosemide blood levels were determined spectrofluorometrically by an extraction method. The area under the blood concentration-time curve AUC0 - , peak blood concentration Cmax and time to reach peak blood concentration Tmax were used to compare their bioavailabilities. The solid dispersion systems produced a higher extent of bioavailability than pure furosemide (P < 0,05). On the contrary, no statistically significant difference about the extent of bioavailability between solid dispersion and Lasix (P > 0,05). Finally, furosemide – PVP solid dispersion (1 : 7) was the best formulation with the highest extent of bioavailability and bioequivalence with the Lasix formula.Key Word : Solid dispersion, solubility, dissolution, bioavailability, furosemide.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/442
10.14499/indonesianjpharm0iss0pp28-32
Indonesian Journal of Pharmacy; Vol 12 No 1, 2001; 28-32
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/442/321
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1442
2019-10-10T01:29:33Z
3:ART
Determination of Classification Model and Total Phenolic Content of Local Variety Soursop (Annona muricata L.) Leaf Powder in Different Altitudes Using NIR and FTIR Spectroscopy with Chemometrics
Wulandari, Lestyo; Department of Pharmaceutical chemistry, Faculty of Pharmacy University of Jember, 68121, Jember, Indonesia
Nugraha, Ari Satia; Department of Pharmaceutical chemistry, Faculty of Pharmacy University of Jember, 68121, Jember, Indonesia
Siswanti, Yashinta Nirmala; Division of Clinical and Community Pharmacy, Faculty of Pharmacy, The University of Jember, 68121, Jember, Indonesia
Annona muricata L., total phenolic Content, NIR, FTIR, chemometric
Soursop (Annona muricata L.) leaves have been widely used traditionally to overcome health problems, this is related to its total phenolic content. This study was aimed to determine the classification model and total phenolic content of soursop leaf powder of local varieties, in different altitudes using NIR and FTIR spectroscopy with chemometrics. Local variety soursop leaf samples that have been collected from low land (0-200 meter above sea level (masl)), medium land (201-700 masl) and high land (>700 masl) are prepared, then scanned using NIR and FTIR spectroscopy. Furthermore, the NIR spectra data from the samples were used as predictors on the LDA classification model of local and queen varieties soursop leaves to identify sample varieties. Samples identified as local varieties, whose total phenolic content was determined using a comparative method (UV-Vis spectrophotometry). The highest mean of total phenolic content is owned by samples from medium land (Jember) of 5.72% w/w GAE, followed by low land (Bangkalan) 2.95% w/w GAE and high land (Batu) 1, 78% w/w GAE. NIR and FTIR spectra data belonging to the samples were analyzed by chemometrics qualitatively using LDA, SVM and SIMCA, and quantitatively using PLS, PCR and SVR. The best classification and calibration model are formed from the NIR spectra data, that are the LDA model with an accuracy of 100% and the PLS model with an R-square calibration value of 0.998071 and RMSEC of 1.2735631. The LDA and PLS models are applied to the real samples. The results of the sample's total phenolic content determination obtained from the NIR spectroscopy method and UV-Vis spectrophotometry method were then tested with Paired-Samples T Test and it can be concluded that the content obtained from the two methods did not have a significant difference.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1442
10.14499/indonesianjpharm30iss1pp7
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 7
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1442/888
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/949
2017-09-11T06:12:36Z
3:ART
ANTIDIABETIC EFFECT EVALUATION AND CHARACTERIZATION OF BIOACTIVE COMPOUNDS ISOLATED FROM HYDROLEA ZEYLANICA
S, Borkar Vijay; Padmavathi College of Pharmacy and Research Institute, Dharmapuri (TN) India.
571/2/3/1 Davkhar Nagar, Chandwad (Nasik) Maharashtra, India- 423101.
K, Senthil Kumaran; K.K. College of Pharmacy, Chennai (TN) India.
KL, Senthil Kumar; Padmavathi College of Pharmacy and Research Institute, Dharmapuri (TN) India.
H, Gangurde Hemant; SSDJ College of Pharmacy, Chandwad (MS) India.
A, Chordiya Mayur; SSDJ College of Pharmacy, Chandwad (MS) India.
The whole plant of Hydrolea zeylanica (HZ) (Hydrophyllaceae family) was coarsely powdered and extracted with ethanol using soxhlet apparatus. The ethanol extract was then fractionated successively using various polarity ranges of solvents and screened for in-vivo antidiabetic activity using streptozotocin induced diabetic male wistar rats. The phytochemical investigation of all the fractions and powdered drug analysis was performed. Among the fractions evaluated, chloroform fraction showed highest decrease in blood glucose, total cholesterol and serum triglyceride level as 75.11%, 59.77% and 35.98% respectively when treated at 50 mg/mL concentration. As the chloroform fraction has shown better potency towards antidiabetic activity, was subjected to chromatographic separation and three compounds stigmasterol, kaempferol and p- coumaric acid were isolated and characterized by various spectroscopic techniques. The overall results tend to suggest the antidiabetic activity of HZ and principal source of presumed bioactive compounds which may be responsible for many of the pharmacological properties.Key word: Hydrolea zeylanica, antidiabetic, total cholesterol, triglycerides, in vivo.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2015-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/949
10.14499/indonesianjpharm26iss4pp185
Indonesian Journal of Pharmacy; Vol 26 No 4, 2015; 185
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/949/741
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1899
2021-01-07T01:00:39Z
3:ART
ANTI-STRESS ACTIVITY OF SOME PLANTS EXTRACTS OF THE NORTH CAUCASUS FLORA
Pozdnyakov, Dmitry Igorevich; Pyatigorsk Medical and Pharmaceutical Institute
Adzhiahmetova, Similla
Chervonnaya, Nadezhda
Mamleev, Andrey
Oganesyan, Eduard
stress; plant extracts; mitochondria
To date, stress is a common medical and socially significant disease that requires rational pharmacotherapeutic correction.The anti-stress properties of ethnolic and aqueous extracts obtained from leaves of Ribes nigrum L., inflorescences of Gaillardia pulchella Foug., stems of Lysimachia punctata L were studied in this work. Acute stress was modeled by immobilization of rats for 2 hours. The test-extracts were administered per os prophylactically in a dose of 1/20 of LD50 (2000 mg/kg). The following parameters were evaluated: organs mass coefficient (adrenal glands, thymus, spleen), the number of stomach erosion; the biochemical changes in the blood serum (adrenaline, cortisol, total protein and glucose concentration); the mitochondrial function parameters in brain and myocardium (evaluation of mitochondrial pore transitional permeability opening and mitochondrial membrane potential). 70% ethanol extract from Gaillardia pulchella Foug. inflorescences has the highest anti-stress activity, the course application of which contributed to the normalization of the weight index of organs, a decrease in glucose concentration by 64.5% (p <0.05), and cortisol by 73.7% (p <0.05) and adrenaline - 78.9% (p <0.05) in the blood, an increase in total protein level by 62.5% (p <0.05) and stabilization of mitochondrial function. The study showed a high anti-stress activity of 70 % ethanol extract from Gaillardia pulchella Foug. inflorescences.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-08-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1899
10.14499/indonesianjpharm31iss2pp131
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 131
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1899/953
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1031
2017-09-11T05:00:54Z
3:ART
PHYSICAL CHARACTERIZATION OF IBUPROFEN-STEARIC ACID BINARY MIXTURE DUE TO COMPRESSION FORCE
Setyawan, Dwi; Dept of Pharmaceutics, Faculty of Pharmacy, Airlangga University, Darmawangsa Dalam Surabaya-Indonesia
Isadiartuti, Dewi; Dept of Pharmaceutics, Faculty of Pharmacy, Airlangga University, Darmawangsa Dalam Surabaya-Indonesia
Betari, Sita Desti; Dept of Pharmaceutics, Faculty of Pharmacy, Airlangga University, Darmawangsa Dalam Surabaya-Indonesia
Paramita, Diajeng Putri; Dept of Pharmaceutics, Faculty of Pharmacy, Airlangga University, Darmawangsa Dalam Surabaya-Indonesia
Aim of this research is to determine physical characteristic of ibuprofen-stearic acid due to compression force. Binary mixture of ibuprofen-stearic acid with weight ratio of 4:6, 5:5, and 6:4 was compressed under various compression force using hydraulic press with 13mm diameter flat punch.Identification of solid state interaction between these two components was performed by Hot Stage Microscopy (HSM). Physical characterization has been studied byDifferential Thermal Analysis (DTA), X-ray Powder Diffraction (XRPD), Scanning Electron Microscopy (SEM), and (Fourier Transform Infrared (FT-IR) spectroscopy. According to hardness measurement, 4:6 weight ratio has the highest tensile strength on 170.6N/cm2 under 19.93kN compression force. Interaction identification by HSM showed single blank line that indicates eutectic formation. Thermal analysis of DTA also revealed eutectic formation upon compression whichthe endotermic peak of ratio of 4:6has the lowest melting temperature of 53.2°C. X-ray diffraction of three peaksgenerally showed that peak intensity decreases as compression force increases, but at particular point it begins to increase again. Sintering phenomenon at the surface of compressed tablet was observed from SEM analysis. FT-IR study confirms the formation of simple eutectic. Key words: ibuprofen, stearic acid, binary mixture, compression force, physical characterization
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1031
10.14499/indonesianjpharm27iss1pp28
Indonesian Journal of Pharmacy; Vol 27 No 1, 2016; 28
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1031/765
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1089
2017-08-02T06:46:30Z
3:ART
ANTIOXIDANT ACTIVITY OF DRIED STRAWBERRIES JUICES (Fragaria vesca L.) EMULGEL PREPARATION USING CANDLENUT OIL AND IT’S DIFFUSION
Erwiyani, Agitya Resti; Faculty of Pharmacy, STIKES Ngudi Waluyo
formulation; emulgel; strawberry juice; difussion; shed snake skin
Strawberries (Fragaria vesca L.) is a fruit that contains nutrients with many benefits and used as an antioxidant because it contains phenolic, ascorbic acid, pelargonidin-3-glucoside and cyanidin-3-glucoside. In addition, strawberries contain flavonoids, which have high antioxidant capacity. Double emulsion can improve the stability of phenolic compounds from dried strawberry juice. Primary emulsion is dispersed in a gel base (emulgel) and it is expected to produce more stable and better preparation with the ability to release the active ingredient topically. The production of double emulsion preparation (A / M / A) using candlenut oil as the oil phase with high level of total flavonoid and total anthocyanins of formula respectively - were 22.86% and 55.915%. The total flavonoid in dried strawberry juice emulgel was able to pass the membrane shed snake skin with higher flux value than the total flavonoid in the form of emulsion type A / M.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1089
10.14499/indonesianjpharm27iss3pp145
Indonesian Journal of Pharmacy; Vol 27 No 3, 2016; 145
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1089/784
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/662
2020-01-27T02:53:58Z
3:ART
SELAGINELLA ACTIVE FRACTIONS INDUCE APOPTOSIS ON T47D BREAST CANCER CELL
Handayani, Sri; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Risdian, Chandra; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Meiyanto, Edy; Faculty of Pharmacy, Universitas Gadjah
Mada, Indonesia. Skip Utara 55281
Yogyakarta
Udin, Zalinar; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Andriyani, Rina; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Angelina, Marissa; Research Center for Chemistry, Indonesian
Institute of Sciences, Indonesia
Apoptosis is an important target on anticancer mechanism. The purpose of this research is to investigate apoptosis induction of Selaginella plana Hieron active fractions on T47D cells. Absolute ethanol was used to extract Selaginella plana powders. Ethanolic extract was dilluted by methanol:water (4:1) and then fractionated by hexane (S_Hex), methylene chloride (S_MTC), ethyl acetate (S_EA), and buthanol (S_BuOH). The proliferation of T47D cell line was detected by SRB (Sulforhodamine B) assay which was measured at a wavelength of 515nm. Flowcytometry analysis to determine apoptosis was examined by Propidium Iodide (PI) and Annexin V assay using T47D breast cancer cell line. The result showed that the IC50 value of S_Hex, S_MTC, S_EA, and S_BuOH on T47D cells were 107 µg/mL, 4 µg/mL, 6 µg/mL, and 17 µg/mL respectively. The active fractions (S_MTC and S_EA) at its IC50 concentration significantly (P<0.05) increased the total number of early apoptotic cells in the T47D cells 3.39% and 4.1% respectively compared to that of control (1.95%). Based on the result, methylene chloride and ethyl acetate fraction of Selaginella plana induced apoptosis on T47D cell.Keywords: apoptosis, breast cancer, Selaginella
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/662
10.14499/indonesianjpharm23iss1pp48-53
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 48-53
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/662/528
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1465
2019-10-28T01:20:02Z
3:ART
The Application of Multiplate Resazurin Reduction Assay in The Screening for Anti-Mycobacterial Activity from Indonesian Medicinal Plants
Sari, Martha; Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI)
Syahputra, Gita; Laboratory for Applied Genetic Engineering and Protein Design, Biotechnology Research Center
Kusharyoto, Wien; Laboratory for Applied Genetic Engineering and Protein Design, Biotechnology Research Center
Anti-mycobacterial, phytochemical extracts, Indonesian medicinal plants, MRRA
Tuberculosis (TB) is an airborne illness generated by Mycobacterium tuberculosis (Mtb), also one of the prominent infectious killers of adults worldwide. There is a pressing need to expand novel anti-mycobacterial drugs because of the increasing resistance of pathogenic mycobacteria to existing antibiotics. Native compounds acquired from microbial resources and medicinal cultivars have played an essential part as the origin of TB medications. The microplate resazurin reduction assay (MRRA) is generally utilized to assess natural and synthetic compounds for anti-mycobacterial activity. In our work, the MRRA method was employed to evaluate the anti-mycobacterial activity of extracts from curative plants using Mycobacterium smegmatis and Mycobacterium bovis BCG and to compare them to rifampicin as an anti-mycobacterial drug. The optimized MRRA utilized 2% aqueous DMSO and 62.5 μg/mL resazurin as an indicator compound in 5% aqueous Tween 80. The optimal incubation time for M. smegmatis was 24 hours, and for M. bovis BCG was 48 hours. The methanolic plant extracts were acquired from various Indonesian medicinal plants known to have anti-mycobacterial activity. The MRRA method using M. smegmatis or M. bovis BCG as anti-mycobacterial targets offers a distinct advantage such as low-cost, rapid, and safe screening for anti-mycobacterial activity in a middle to high-through-put-format.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ)
Indonesia Institute of Sciences (LIPI)
2019-08-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1465
10.14499/indonesianjpharm30iss3pp199
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 199
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1465/911
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1056
2017-09-11T06:10:09Z
3:ART
Modulatory effect of Drosera peltata J.E.Sm on development of metabolic syndrome in tumor bearing mice
A, Raju
Raj, Seeja S
AJM, Christina
Metabolic syndrome; Ehrlich's Ascites Carcinoma; Dalton’s ascites lymphoma; Drocera peltata; anticancer
The purpose of the study conducted was to know the extent of protection over the cancer associated metabolic syndrome development after administration of ethanol and aqueous extracts of Drocera peltata against Dalton’s ascites lymphoma (DAL) and Ehrlich's Ascites Carcinoma (EAC) bearing mice. Animals were divided into thirteen groups with a normal control, EAC control, DAL control, two groups with standard drug 5-Flurouracil 20mg/kg+ DAL & EAC and eight groups with 250 and 500 mg/kg of ethanol and aqueous extracts of D.peltata + EAC & DAL, for respective cell lines. After 24 hours of both tumor cell inoculation, animals were treated with extracts,once in a day for 14 days continuously. The indicators for the development of metabolic syndrome such as changes in blood glucose, serum hormone and lipid profile were found with both cell line bearing mice. Both ethanol and aqueous extracts of D.peltata at doses of 250 and 500mg/kg significantly reduced the elevated blood glucose, hormonal and lipid profile changes. These results confirmed that ethanol and aqueous extracts can stabilize the tumor induced hormonal, blood glucose and lipid profile changes in tumor bearing mice. This effect might be due to the presence of pharmacologically active phytoconstituents in extracts.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-12-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1056
10.14499/indonesianjpharm27iss4pp203
Indonesian Journal of Pharmacy; Vol 27 No 4, 2016; 203
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1056/794
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1056/96
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/811
2017-09-12T07:25:03Z
3:ART
ANTIHYPERGLYCEMIC, ANTIOXIDANT, AND PANCREAS PROTECTIVE EFFECTS OF CORIANDRUM SATIVUM SEED IN ALLOXAN-INDUCED DIABETIC RATS
Widodo, Gunawan Pamudji; Faculty of Pharmacy, Setia Budi University, Surakarta, Indonesia.
Jalan Letjen Sutoyo Mojosongo, Surakarta, 57127. Indonesia
Handayani, Sri Rejeki; Faculty of Pharmacy, Setia Budi University, Surakarta, Indonesia.
Jalan Letjen Sutoyo Mojosongo, Surakarta, 57127. Indonesia
Hierowat, Rina; Faculty of Pharmacy, Setia Budi University, Surakarta, Indonesia.
Jalan Letjen Sutoyo Mojosongo, Surakarta, 57127. Indonesia
Coriandrum sativum is a medicinal plant, used in traditional medicine for diabetes therapy. The goal of the this study was to determine the antidiabetic, antioxidant and pancreas protection effects of ethanol extracts of C. sativum seeds (CSE) in alloxan-induced diabetic rats. The male Wistar rats were induced diabetic by intraperitoneal injection of alloxan (150mg/kg BW). CSE was prepared and administrated orally to the animals at the dose of 125 and 250mg/kg for 28 days. Blood glucose level was measured, and antioxidant status was assessed by determining the activities of superoxide dismutase, glutathione peroxidase as well as malonyl aldehyde in liver. Histopathological study of pancreas was conducted at the end of experimental period. Both dose of SCE showed the glucose lowering effect, corrected antioxidant status of diabetic animals in liver and protected the pancreas organ from damage. Key words: Coriandrum sativum seed, antihyperglycemia, antioxidant, pancreas protection
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2015-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/811
10.14499/indonesianjpharm25iss3pp129
Indonesian Journal of Pharmacy; Vol 26 No 3, 2015; 129
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/811/750
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1323
2018-10-23T02:19:33Z
3:ART
Isolation and Identification of α-Glucosidase Inhibitor From Aspergillus Terreus F38
Munasaroh, Siti; Universitas Pancasila
Tamat, Swasono R; Pancasila University
Dewi, Rizna Triana; Chemistry Research Center – LIPI
α-glukosidase inhibition, Aspergillus terreus F38, butyrolactone III.
Controlled postprandial glucose level is an important strategy in preventing DM type 2. Inhibitors of α-glucosidase have been postulated to be useful agents in managements of DM type 2. This research aims to isolate and identify of α-glucosidase inhibitor fromAspergillus terreus F38 by liquid fermentation. The mycelium extract of A. terreus F38showed strong activity against α-glucosidase with IC50 value of 9.65 μg/mL. Separation and purification of mycelium ectract yielded compound I (Butyrolactone III). The structure was establish on the basis of spectral analysis, according to the data obtained by NMR and LCMS-MS experiments. Compound I showed potential activity against α-glucosidase with IC50 value of 13.87 μg/mL. Therefore, the metabolites from A. terreus F38 can be used as lead compound to design potent α-glucosidase inhibitory agents.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-07-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1323
10.14499/indonesianjpharm29iss2pp74
Indonesian Journal of Pharmacy; Vol 29 No 2, 2018; 74
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1323/869
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/679
2020-01-27T02:53:16Z
3:ART
QSAR MODELING OF 2-[CH(OH)X]-5,8-(OY)2 -1,4-NAPHTHOQUININES AGAINST L1210 CELLS USING MULTIPLE LINEAR REGRESSION
., Ajeet; Department of Pharmaceutical Chemistry,
S. D. College of Pharmacy and Vocational Studies, Muzaffarnagar, U.P. 251001
India
Singh, Brajpal; Department of Pharmaceutical Chemistry,
S. D. College of Pharmacy and Vocational Studies, Muzaffarnagar, U.P. 251001
India
Kumar, Vipul; Department of Pharmaceutical Technology, Meerut Institute of Engineering and Technology, Meerut, U.P., India
Quinones are present in many drugs such as anthracyclines, daunorubicin, doxorubicin, mitomycin, mitoxantrones and saintopin, which are used clinically in the therapy of solid cancers. The cytotoxic effects of these quinone are mainly due to the inhibition of DNA topoisomerase-II. It is the necessity to develop the 1,4-Naphthoquinone analogues with Cytotoxic effect. Here 2-[CH(OH)X]-5,8-(OY)2-1,4-Naphthoquinines analogues have been used to correlate the cytotoxic activity with the Eccentric Connectivity index (ECI), Fragment Complexity (FC) and McGowan Volumes (MG) for studying the Quantitative Structure Activity Relationship (QSAR). Correlation may be an adequate predictive model which can help to provide guidance in designing and subsequently yielding greatly specific compounds that may have reduced side effects and improved pharmacological activities. We have used Multiple Linear Regression (MLR), one of the best methods for developing the QSAR model. Results from this QSAR study have suggested that ECI, FC and MG are the important descriptors for cytotoxic activities of 1,4-Naphthoquinones against L1210 cells. For the validation of the developed QSAR model, statistical analysis such as data point-descriptor ratio, fraction of variance, cross validation test, standard deviation, quality factor, Fischer’s test; and internal validation such as Y-randomization test have been performed and all the tests validated this QSAR model.Key words: 1,4-Naphthoquinones, QSAR, Eccentric connectivity index, Fragment complexity, McGowan Volume, Multiple Linear Regression
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/679
10.14499/indonesianjpharm23iss3pp171-176
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 171-176
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/679/545
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1863
2022-10-26T01:05:53Z
3:ART
Effects of Peel Extract from Citrus reticulata and Hesperidin, A Citrus Flavonoid, on Macrophage Cell Line
Ikawati, Muthi’
Armandari, Inna
Khumaira, Annisa
Ertanto, Yogi
Citrus reticulata, hesperidin, macrophage RAW 264.7 cell, proliferation, interleukin-10 (IL-10)
The extract of Citrus reticulata has been studied for its biological activities, due to its citrus flavonoid content. The extract and its flavonoid compounds exhibit growth inhibition properties in several cancer cell lines and in vivo models. Conversely, the extract can also induce cell proliferation and angiogenesis, and shows estrogenic effects, in vitro and in vivo. Because of the contrasting effects that depend on the concentration or dosage, the precise action of the extract and its flavonoids need to be elucidated in various cell types. The objective of this study is to evaluate the effect of Citrus reticulata peel extract (Citrus extract) and hesperidin, a citrus flavonoid, on the modulation of cell proliferation in the RAW 264.7 macrophage cell line. Cell viability under Citrus extract or hesperidin treatment was assessed by using the MTT assay. The expression of interleukin-10 (IL-10), an anti-inflammatory cytokine, modulated by Citrus extract was also examined by immunostaining. Low concentrations of Citrus extract at 1 and 100 μg/mL were able to induce cell proliferation, though not significantly, as shown by cell viability of 138 and 114%, respectively. At higher concentrations of 500, 750, and 1000 μg/mL, Citrus extract decreased cell viability significantly by up to 64, 46, and 36%, respectively. Accordingly, hesperidin at low (3.1 μg/mL−61.1 μg/mL) or high (152.6 μg/mL−305.3 μg/mL) concentrations increased or reduced cell viability significantly by up to 116−136% or 10−61%, respectively. The value of the 50% inhibitory concentration (IC50) of Citrus extract was more than three times higher (756 μg/mL) than that of hesperidin (203 μg/mL = 332 μM). Additionally, 250 μg/mL of Citrus extract was able to induce IL-10 expression compared with the control. These results demonstrate that Citrus extract and hesperidin exert a biphasic effect on macrophage cells. The future development of Citrus extract as a co-chemotherapeutic, anticancer, or immunomodulatory agent should include careful consideration of its biphasic effect on each cell type.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-02-19
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1863
10.14499/indonesianjpharm30iss4pp260
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 260
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1863/935
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1422
2019-10-03T03:16:50Z
3:ART
Effects of Antioxidant, Anti-Collagenase, Anti-Elastase, Anti-Tyrosinase of The Extract and Fraction From Turbinaria decurrens Bory.
Nurrochmad, Arief; Dept of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta 55281, Indonesia
Wirasti, Wirasti; Post Graduate Student of Faculty of Pharmacy Universitas Gadjah Mada
Dirman, Arifin; Post Graduate Student of Faculty of Pharmacy Universitas Gadjah Mada
Lukitaningsih, Endang; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta 55281, Indonesia
Rahmawati, Adillah; Post Graduate Student of Faculty of Pharmacy Universitas Gadjah Mada
Fakhrudin, Nanang; Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara Yogyakarta
Turbinaria decurrens Bory.; fucoxanthin; antioxidant; anti-collagenase; anti-elastase; anti-tyrosinase
Brown macroalgae (BM) which contain fucoxanthin exhibited high antioxidant activity. This study was performed to examine antioxidant, anti-collagenase, anti-elastase, anti-tyrosinase activities, and effect on cell viability of Human Dermal Fibroblast adult (HDFa) of BM, Turbinaria decurrens Bory. T. decurrens dried powder were macerated by ethanol to obtain extract (ETD) and was fractination by column chromatography to obtain fraction (FTD). Fucoxanthin content was determined using HPLC. The antioxidant activities, anti-collagenase, anti-elastase, and tyrosinase inhibitory assay were performed. The effect of ETD and fucoxanthin standard on cell viability were conducted on HDFa cell-induced by hydrogen peroxide (H2O2). The HPLC analysis showed that ETD and FTD contain fucoxanthin of 284.9±3.3µg/g and of 653.4±30.6µg/g dry-weight, respectively. The antioxidant assay showed that ETD and FTD produced high antioxidant activity by ferric reducing antioxidant power (FRAP) and b-carotene bleaching (BCB) methods that were comparable to fucoxanthin. ETD exhibited significantly higher tyrosinase inhibitory than kojic acid (p<0.01), while FTD had a comparable effect to kojic acid. The result also revealed that ETD and FTD produced anti-elastase and anti-collagenase (matrix metalloproteinase-1 (MMP-1). Fucoxanthin and ETD were able to maintain cell viability on HDFa cell-induced H2O2. This study suggests that T. decurrens may be effective to prevent skin aging and wrinkle formation, possibly through the antioxidant activity and maintain cell viability of fibroblast.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-12-17
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1422
10.14499/indonesianjpharm29iss4pp188
Indonesian Journal of Pharmacy; Vol 29 No 4, 2018; 188
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1422/883
Copyright (c) 2018 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/690
2020-01-27T02:53:31Z
3:ART
DEVELOPMENT THE VALIDATION OF INDONESIAN VERSION OF SF-36 QUESTIONNAIRE IN CANCER DISEASE
Perwitasari, Dyah Ariani; Faculty of Pharmacy, Ahmad Dahlan University
Jl. Kapas No.9, Semaki, Yogyakarta, Indonesia
55166.
A Cancer patients need long term therapy in the treatment of their disease. One of the outcome of cancer treatment in patients of quality of life. Because of the limited instrument for measuring cancer patients’ quality of life in Indonesian version, thus this study is aimed to develop the validation of Indonesian version of Short Form-36 questionnaire in cancer patients. The observational study was carried out in this study. Data were collected from cancer patients in the Oncology Department of Sardjito Hospital, Yogyakarta, Indonesia, who were treated with cisplatin at the dosage of ≥ 50mg/m2 as monotherapy or in combinations. The validation process involved known-group validity, discriminant validity, convergent validity and factor analysis. About 203 subjects who were diagnosed with cancer were recruited in this study. Theknown-group validity test showed that there were no significant differences of SF-36 domains among different diagnosa. All of the questions met the criteria of convergent validity except for the questions number 26, 28, 30 and 32. Furthermore, the questions number 2 and 28 did not meet the criteria of discriminant validity. This study presents that many questions are needed to be reconstructed, due to the result of factor analysis which showed that those questions are loaded significantly with other domain’s questions. The development of Indonesian version of SF-36 scales in cancer disease is still less unstatisfactory. According to the result of this study, future study with new construction of questions in this version is still necessary.Key words: validation, SF-36, Indonesia, cancer
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/690
10.14499/indonesianjpharm23iss4pp248-253
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 248-253
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/690/556
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1810
2021-01-07T01:00:39Z
3:ART
QuEChERS and C18 as solid phase extraction sorbent - ultra-high performance liquid chromatography -ultraviolet-visible method for determination of parabens in cosmetics products
Nguyen, Ngoc-Van Thi; Can Tho University of Medicine and Pharmacy
paraben, cosmetic, QuEChERS, solid phase extraction
Concerns are growing about human exposure to endocrine-disrupting chemicals (EDCs), especially during the preadolescent development stage. Parabens are prevalent EDCs widely used as additives in cosmetics. So, the determination of parabens in such products is important. In this study, we developed a reliable and sensitive method to determine simultaneously nine common parabens (methylparaben, ethylparaben, phenylparaben, benzylparaben, penthylparaben, and two groups of isomeric compounds include propylparaben, isopropyl paraben, and butylparaben, isobutylparaben) in cosmetics products. The QuEChERS and solid-phase extraction techniques are used for extraction parabens from non-surfactant cosmetics (perfume, mouth wash solution) and surfactant cosmetics (shampoo, cream, gel), respectively and quantified by using ultra-performance liquid chromatography coupled with the ultraviolet-visible detector. All nine compounds showed good linearity with regression coefficients predominantly above 0.990. The LOD and LOQ of parabens were 0.07 µg/mL; 0.2 µg/mL, respectively. The recoveries ranged from 80 to 110% with the relative standard deviations below 8%. The developed method was successfully applied to determine parabens in various commercial cosmetic products from a local supermarket and the total parabens concentrations are in a wide-ranged from 2.0 to 1270 mg/kg.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-08-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1810
10.14499/indonesianjpharm31iss2pp69
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 69
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1810/950
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1084
2017-09-11T06:09:26Z
3:ART
FORMULATION OF SELF-NANOEMULSIFYING DRUG DELIVERY SYSTEM OF BOVINE SERUM ALBUMIN USING HLB (HYDROPHILIC-LYPOPHILIC BALANCE) APPROACH
Winarti, Lina; Postgraduate Programme, Faculty of Pharmacy, Universitas Gadjah Mada
SNEDDS; BSA; hydrophllic surfactant; lypophilic surfactant; protein delivery
Self-Nanoemulsifying Drug Delivery System (SNEDDS) has potential to be developed for oral protein delivery because it is free from water, hence preserving the stability of protein, protecting protein from enzymatic degradation, and enhancing the protein permeability in the gastrointestinal tract (GIT). However, protein-based SNEDDS formulation is challenging due to low solubility property of protein in oil, which is towards zero. This present study aimed to obtain the most compatible SNEDDS system for protein using HLB approach. Bovine serum albumin (BSA) was used as a protein model in this study. A number of 78 formulae with HLB ranging between 11 and 15 were screened to acquire stable SNEDDS composition without the presence of phase separation. Of 13 stable formulae, two were selected (F30, F45) with HLB 15, and then loaded with BSA. Physical characteristics of both formulae were then evaluated and the results suggested that SNEDDS with single hydrophilic surfactant (F45) and HLB 15 was the best formula for protein template as the stability testing showed that phase separation and precipitation did not appear. It was robust to pH and dilution with percentage of transmittance of 96.40±1.05% and the droplet size of 180.9nm. F45 also had uniform distribution of droplets size since the polydispersity index was less than 0.1. The zeta potential of F45 was -0.12mv with loading efficiency 83.57±1.77%. The emulsifying time of F45 was > 2min due to the formation of crystalline gel that was difficult to disperse.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1084
10.14499/indonesianjpharm27iss3pp117
Indonesian Journal of Pharmacy; Vol 27 No 3, 2016; 117
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1084/789
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/514
2018-09-05T01:49:55Z
3:ART
EFFECTS OF AVURVEDIC SHODHANA (PROCESSING) ON DRIED TUBEROUS ACONITE (Aconitum napellus Linn.) ROOT
Paul, Arindam; Department of Pharmacology and Pharmacognosy, Babaria Institute of Pharmacy, BITS edu campus, Vadodara-Mumbai NH#8, Varnama, Vadodara - 391240, Gujarat, INDIA
Gajjar, Umang; Department of Pharmacology and Pharmacognosy, Babaria Institute of Pharmacy, BITS edu campus, Vadodara-Mumbai NH#8, Varnama, Vadodara - 391240, Gujarat, INDIA
Donga, Jignasa; Department of Pharmacology and Pharmacognosy, Babaria Institute of Pharmacy, BITS edu campus, Vadodara-Mumbai NH#8, Varnama, Vadodara - 391240, Gujarat, INDIA
Aconite (Aconitum napellus Linn.) commonly known as atis is a poisonous plant used extensively as antihypertensive, antipyretic, analgesic and antirheumatic. Ayurveda recommended the administration of aconite roots only after purification, i.e., boiling roots in cow’s urine (Gomutra). In the present study an attempt was made to compare the pro-arrhythmic and antihypertensive effects of powdered aconite root purified by shodhana process with that of unpurified form of aconite roots in order to provide scientific support of the claim in ayurvedic texts that purification of aconite root by shodhana process retains its antihypertensive activity and is devoid of pro-arrhythmic activity. Aconite root treatment in both forms purified and unpurified) caused significant reduction in BP when compared with diseased control group (P<0.05). The unpurified aconite root group showed significant increase in heart rate, increase in QRS complex time and increase in QT interval, however these parameters were statistically insignificant in purified aconite root treated group. The PRA, SC and BUN levels was significantly decreased in aconite root treatment groups. The probable mechanism of antihypertensive activity of aconite root can be attributed to decrease in plasma renin activity, decrease in oxidative stress and increase in NO levels. Key words: Aconite, shodhana process, antihypertensive, proarrhythmic
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2013-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/514
10.14499/indonesianjpharm24iss1pp40-46
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 40-46
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/514/392
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1464
2019-10-10T01:30:23Z
3:ART
Citalopram and Tianeptine: Pharmacological Interventions in alcohol withdrawal-induced negative mood states and serotonin insufficiency in Albino Wistar male rats
Amjad, Iffat Ara; Minhaj University Lahore
Abdul Rub, Samina Bano; University of Karachi
Serotonin, Alcohol withdrawal syndrome, Serotonergic antidepressants. Tryptophan metabolism
ABSTRACT The present study aims to investigate the neurochemical and behavioural effects of tianeptine and citalopram to block alcohol withdrawal-induced audiogenic seizures in alcohol withdrawal (AW) rats. Citalopram and tianeptine were administered sub-chronically to block AW-induced audiogenic seizures. Brain regional tryptophan (TRP), 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA) concentrations were determined using high-performance liquid chromatography connected to the fluorimetric detector. Citalopram increased brain TRP concentration in all the regions, however, tianeptine increased brain TRP concentration only in amygdala and hippocampus. Increased brain serotonin concentration was seen by citalopram but not by tianeptine in all the regions. Further, tianeptine was shown to increase 5-HT turnover in all the regions, however, citalopram appeared to increase 5-HT turnover only in the hippocampus. It is concluded that the citalopram and tianeptine behave differently on the intrinsic pathway of serotonin metabolism that appeared to be compensated by the release pattern of serotonin. Further, chronic exposure of serotonergic agents causes restoration and structural reforming of serotonin reuptake mechanism that is desensitized following chronic alcohol exposure. Thus the inclusive approach of the serotonergic system plays an undoubted role in the pharmacological management of AW syndrome.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Higher Education Commission,Pakistan
2019-06-18
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1464
10.14499/indonesianjpharm30iss2pp75-83
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 75-83
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1464/906
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1119
2017-09-11T06:08:59Z
3:ART
Validation and Quantification of Theophylline and Salbutamol using Ion Pair Liquid Chromatography
Sophi, Shanti Laksmi; Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, 55281 Indonesia
Martono, Sudibyo; Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, 55281 Indonesia
Rohman, Abdul; Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, 55281 Indonesia
Salbutamol and theophylline are administered in combination orally to provide the sinergistic effect of drugs. The aim of this study is to develop and to validate ion pair liquid chromatography to determine both drugs in pharmaceutical dosage form, especialy in syrup dosage form. Separation of drugs was performed with Spherisorb C-18 column (250 x 4.6 mm; 10 um). The mobile phase used is the mixture of acetic acid 1% : methanol (60 : 40 v/v) which contain 3.5 mM sodium-1-octanesulphonate, as ion pairing reagent. The mobile phase was delivered isocratically with flow rate of 1 mL/min. UV detection was set at wavelength of 277 nm. The developed method was validated in terms of linearity, precision, accuracy, selectivity, and sensitivity. The precision of the method was evaluated using repeatability assay having relative standard deviation (RSD) values of 0.41–0.70 % for theophylline and 0.08 – 0.24 % for salbutamol. The recovery percentages are in the range of 98.28–101.02% (theophylline) and 100.71–101.60% for salbutamol. The developed method meets the validation requirement for analysis of salbutamol and theophylline simultaneously in syrup dosage form. Furthermore, the method also provides acceptable result for syrup and tablet dosage forms containing Salbutamol and theophylline only, and tablet containing the combination of Salbutamol and theophylline.Keywords: teophyllin, salbutamol, HPLC, sodium-1-octanesulphonate, validation.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-09-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1119
10.14499/indonesianjpharm27iss4pp190
Indonesian Journal of Pharmacy; Vol 27 No 4, 2016; 190
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1119/792
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/661
2020-01-27T02:53:58Z
3:ART
PREDICTORS OF THE USE OF GASTROPROTECTIVE AGENTS IN PATIENTS USING NSAID IN YOGYAKARTA INDONESIA
Indrianto, Ady Bagus; Postgraduate Program, Faculty of Pharmacy,
Universitas Gadjah Mada
Thobari, Jarir At; Department of Pharmacology and Toxicology, Faculty of Medicine, Universitas Gadjah Mada
Nugroho, Agung Endro; Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta Indonesia 55281
Non Steroid Anti-Inflammatory (NSAID) is one type of AntiInflammatory drug that is used to treat pain in patients of osteoarthritis (OA) and rheumatoid arthritis (RA). The use of NSAIDs can cause gastrointestinal side effects. To prevent the side effects, NSAIDs are prescribed in combination with gastroprotective agent (GPA). The aim of the study is to determine the factors considered in the GPA prescription by the doctors in patients who have risk factors for gastric disorder due to NSAIDs use. This study was performed using a cross-sectional observational design. The data were collected retrospectively during a period of January to December 2010. The subjects were OA and RA patients considering the inclusion and exclusion criteria. The data included patient characteristics (gender, age, occupation, payment method, the primary diagnosis), prescription of NSAID, prescription of GPA, history of gastrointestinal disease, NSAID prescription with corticosteroids, prescription with antiplatelet. The data were processed descriptively and quantitatively, and analyzed statistically using chi-square and log. regression with 95% Confidence Interval. The results showed that there were correlation between some predictor factors with prescription of gastroprotective agent. These factors were women patient, patient age of ≥ 65 years, oxicam prescribing, diclofenac sodium prescribing, prescribing >1 type of NSAIDs, prescription of NSAID concomitant with corticosteroids, prescription of NSAID concomitant with antiplatelet, patients with history of gastrointestinal, prescribing NSAIDs with duration of ≥ 3 months. Patient with a history of gastrointestinal disease was the most predictor influential factor, with OR (odds ratio) of 3.6 (95% CI: 2.79 - 4.66). Predictor factor of patients with a history of dyspepsia possessed the highest OR {OR=4,29 (CI 95%: 3.23–5.7)}. It means that patients with a history of dyspepsia prescribed NSAIDs would have greater risk of 4.29 times to get GPA prescription than patients without a history of dyspepsia. Prescribing NSAIDs > 1 DDD (defined daily dose) had p value of 0.777, which means there was no relationship between GPA prescription with NSAIDs > 1 DDD prescription.Key words: Gastroprotective Agent, Non-steroid anti-inflammatory, Osteoarthritis, Rheumatoid Arthritis
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/661
10.14499/indonesianjpharm23iss1pp41-47
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 41-47
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/661/527
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1285
2018-10-23T02:19:33Z
3:ART
Physical Characterization and Dissolution Study of Pentagamavunon-0 Loaded Self Nano-Emulsifying Drug Delivery System
Astuti, Ika Yuni; Universitas Muhammadiyah Purwokerto
Marchaban, Marchaban; Universitas Gadjah Mada
Martien, Ronny; Universitas Gadjah Mada
Nugroho, Agung Endro; Universitas Gadjah Mada
PGV-0 SNEDDS; characterization; dissolution
The recent work focuses on the physical characterization and dissolution study of PGV-0 loaded self-nanoemulsifying drug delivery system (SNEDDS). The PGV-0 SNEDDS was prepared by spontaneous emulsification method using oleic acid, tween 20:labrasol (1:1) and polyethylene glycol 400. The zeta potential of the PGV-0 SNEDDS was -34.4 ± 7.66 mv. The nanoemulsion of PGV-0 SNEDDS was thermodynamically stable with forming droplet oil containing PGV-0 in it and spherical. The dissolution result showed that the PGV-0 SNEDDS was able to increase the dissolution significantly (p <0.05) compared with PGV-0 powder of 44.13% in AGF medium and 30.37% in AIF medium. It is concluded that SNEDDS PGV-0 was able to increase the PGV-0 aqueous solubility.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-06-22
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1285
10.14499/indonesianjpharm29iss2pp60
Indonesian Journal of Pharmacy; Vol 29 No 2, 2018; 60
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1285/871
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/673
2020-01-27T02:51:49Z
3:ART
PASSIVE AND IONTOPHORETIC PERMEATION OF CAPTOPRIL GEL: AN IN VITRO AND IN VIVO STUDY
Jain, Ashish; Bansal College of Pharmacy, Kokta, Anand Nagar, Bhopal-462021, India.
Nayak, Satish; Bansal College of Pharmacy, Kokta, Anand Nagar, Bhopal-462021, India.
Soni, Vandana; Department of Pharmaceutical Sciences, Dr H.S.Gour Vishwavidyalaya, Sagar 470003, M.P. India
The Objective of this work was to formulate and evaluate captopril gel to assess its suitability for transdermal delivery by passive and iontophoresis. A polymer gel was prepared using hydroxypropyl methyl cellulose and in vitro skin permeability was assessed in full thickness skin of rabbits and pigs. For in vivo studies New Zealand rabbits were used. In vitro passive permeation was carried out in Franz diffusion cell but for iontophoresis, diffusion cell was modified according to Glikfield design. Iontophoresis was performed at a current density of 0.5 mA/cm2via silver /silver chloride electrodes with passive controls but for in vivo study current density wasreduced to 0.1 mA/cm2. Blood samples were analyzed for drug content by HPLC. Results of the in vitro study indicated that iontophoresis considerably increased the permeation rate of captopril compared to passive controls in both the skin types (P<0.01). The plasma concentration of captopril was significantly higher (P<0.001) than that obtained in the passive controls. Results showed that the target permeation rates for captopril could be achieved with the aid of iontophoresis by increasing the area in an appreciable range.Key words: Captopril, iontophoresis, transdermal, Rabbit, Pigskin, in vitro, in vivo.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/673
10.14499/indonesianjpharm23iss2pp121-129
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 121-129
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/673/539
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1698
2022-10-26T01:05:53Z
3:ART
Pharmacoeconomic Rationale of Zinc Supplementation In The Management of Acute Diarrhea In Children With Rotavirus Infection In Indonesia
Hakim, Mohamad Saifudin; Department of Microbiology, Faculty of Medicine, Public Health and Nursing
Soloviov, Serhii
Soenarto, Yati; Department of Chemistry IPB University, Bogor
Dzyublyk, Iryna
Leleka, Maryia
Trokhymchuk, Viktor
iarrhea, modelling, rotavirus, treatment, zinc supplementation
Rotavirus is one of the most important causative agents of gastroenteritis in both infants and children worldwide, resulting in a high mortality and burden of disease, mainly in low-income, developing countries. Oral rehydration therapy with zinc supplementation is currently the recommended way to prevent death from diarrheal dehydration. In this study, we aimed to estimate the effectiveness of zinc supplementation in combination therapy of patients with suspected rotavirus infection in Indonesia. The perspective of this pharmacoeconomic study refer to the economic vantage point of cost-of-illness analysis. Achievement of this goal was possible with the construction of a decision tree model and determination of decision rules for inclusion of zinc supplementation into the combination therapy. The input parameters of the model were hospitalization days of patients stratified by the presence or absence of rotavirus infection as well as the additional inclusion of zinc supplementation. The criterion for prediction and decision making was the global rotavirus prevalence. The feature of the simulation was that the costs were expressed as relative to each other, which allowed to unify the proposed methodology. Retrospective analysis of clinical database of Indonesian patients with acute diarrhea has shown that zinc supplementation would be rational in case of rotavirus prevalence among these patients is higher than 81.5%. It was shown that additional zinc supplementation would be costeffective with probability of 0.62 and 0.53 – in the positive range of cost ratio variation or if costs for less than 43% of baseline therapy per day costs on an average.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Directorate of Research and Community Service, Ministry of Research Technology and Higher Education of the Republic of Indonesia for financial support of this research by Penelitian Fundamental Grant 2019 (No: 4167/IT3.L1/PN/2019).
2020-01-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1698
10.14499/indonesianjpharm30iss4pp285
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 285
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1698/926
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1099
2017-09-11T06:19:57Z
3:ART
Hepatoprotective Activity of Ethyl Acetate Fraction of Senggugu’s Root Bark (Clerodendrum serratum L.Moon) on Rats Induced by CCl4
Nasrudin, Nasrudin; Student of Doctoral Program in Pharmaceutical Sciences, University of Gadjah Mada, Yogyakarta
Haluoleo University, Kendari, Southeast Sulawesi
Wahyono, Wahyono; Faculty of Teacher Training
and Education, Halu Oleo University,
Kendari, Southeast Sulawesi
93232
Mustofa, Mustofa; Department of Pharmacology and
Therapy, Faculty of Medicine, Universitas
Gadjah Mada, Sekip Utara,
Yogyakarta 55281
Asmah, Ratna; Department of Pharmaceutical Chemistry,
Faculty of Pharmacy, Universitas Gadjah
Mada, Sekip Utara, Yogyakarta
55281
Clerodendrum serratum; antioxidant; hepatoprotective effect; carbon tetrachloride
Senggugu is a plant that has long been used to treat syphilis, typhoid, cancer, jaundice, and hypertension. The pharmacological activity of senggugu in Indonesia that have been reported include antifertility activity in leaves, mucolytic activity, anti-inflammatory and tracheospasmolytic, also antioxidant in its root bark. This study aims to determine the hepatoprotective effect of ethyl acetate extract fraction of senggugu’s root bark in rats induced by CCl4. The powder of senggugu’s root bark was extracted by terraced maceration method starts from n-hexane, ethyl acetate, to methanol, thus obtained ethyl acetate extract fraction of senggugu’s root bark (FEAKAS). The ethyl acetate extract fractions were then tested for hepatoproctective activity using doses of 25, 50, and 100 mg/Kg.BW on rats induced by CCl4. FEAKAS hepatoprotective activity was determined from the analysis of blood biochemical and oxidative stress parameters. The blood biochemical parameters included SGOT (serum glutamic oxaloacetic transaminase), SGPT (serum glutamic pyruvic transaminase), ALP (alkaline phosphatase), bilirubin, and total protein were measured with test kit. The oxidative stress parameters were measured from homogenates of liver tissue that were prepared by adding 500 mL of 50 nM Tris buffer (pH 7.4) containing 1 mM EDTA and 10 µg/mL leupeptin. The homogenates were centrifuged to obtain supernatants for measurement of oxidative stress parameters using spectrophotometer method, including MDA (malondialdehyde), GPx (glutathione peroxidase) and CAT (catalase). The results showed that the effect of FEAKAS against CCl4 induction for preventing lipid peroxidation, from both blood chemical and oxidative stress parameters, are shown at a dose of 100 mg/Kg.BW that significantly different compared to CCl4 control (ρ <0.05) on all blood chemical and oxidative stress parameters.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-08-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1099
10.14499/indonesianjpharm28iss1pp10
Indonesian Journal of Pharmacy; Vol 28 No 1, 2017; 10
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1099/817
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/413
2017-09-20T03:27:59Z
3:ART
Synthesis of Tetrahydro Pentagavunon-0
Ritmaleni, Ritmaleni; Faculty of Pharmacy, Universitas Gadjah Mada. Yogyakarta, Indonesia.
Simbara, Ari; Faculty of Pharmacy, Universitas Ahmad Dahlan. Yogyakarta, Indonesia.
Curcumin, tetrahydrocurcumin, and monoketone curcumin analogue, pentagamavunone-0 (PGV-0), have been investigated as antioxidant, antiinflammatory and anticancer. This research was aimed to synthesise the Tetrahydropentagamavunone-0 (THPGV-0) compound which assumed as an active metabolite of PGV-0. The hydrogenation reaction was applied to the synthesis of THPGV-0 from PGV-0 using Pd/C 10 % as catalyst at room temperature. The structure elucidation was analysed by using spectroscophy method. The synthetic result showed that THPGV-0 asa white crystalline powder in 25 % with melting point about 122-123 oC.Key words: pentagamavunone-0, Tetrahydropentagamavunone-0, hydrogenation
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2010-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/413
10.14499/indonesianjpharm0iss0pp100-105
Indonesian Journal of Pharmacy; Vol 21 No 2, 2010; 100-105
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/413/293
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1417
2019-10-10T01:29:33Z
3:ART
Nanocurcumin Preparation for Reducing Vcam-1 and IL-6 in High Fat Diet-Induced Hyperlipidemic Rats
Pradana, Dimas Adhi; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia Jl. Kaliurang, km. 14,5 Yogyakarta 55584 Indonesia
Ardhi, Maulana; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia Jl. Kaliurang, km. 14,5 Yogyakarta 55584 Indonesia
Hasyono, Arifa Caryn Dea Utami; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia Jl. Kaliurang, km. 14,5 Yogyakarta 55584 Indonesia
Meytasari, Dzihni; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Islam Indonesia Jl. Kaliurang, km. 14,5 Yogyakarta 55584 Indonesia
Nabilah, Farah Deastasa
Istikharah, Rochmy
Chabib, Lutfi
Atherosclerosis, curcumin, SNEDDS, nanosuspension, nanoemulsion, solid lipid nano, VCAM-1, IL-6, preventive measure
Hyperlipidemia is a pathological condition due to lipid metabolism abnormalities. Increased and oxidized LDL in hyperlipidemia will trigger an inflammatory response and produce proinflammatory cytokines, such as vascular cell adhesion molecule-1 (VCAM-1) and Interleukin-6 (IL-6). Curcumin can be used as antioxidants, anti-inflammatory and antihyperlipidemia, but as it is practically insoluble in water, formulation of curcumin nanosuspension is made to improve the effects of curcumin therapy. This study was conducted to determine the activities of nanocurcumin preparation as preventive measures for rats induced with hyperlipidemia. The test animals used were 49 male Wistar rats divided into 7 test groups: normal control group, negative control group, 80mg/kg BW/day curcumin control group, and nanosuspension curcumin dose of 80mg/kg BW/day, nanoemulsion curcumin dose of 80mg/kg BW/day, SNEDDS curcumin dose of 80mg/kg BW/day and solid lipid nanoparticles dose of 80mg/kg BW/day. Provision of preventive measure was performed on days 1-67. On day 11-67, the induction of lard and egg yolk (1:1) was administered with a volume of 2mL/200g of rat BW. On the 68th day, blood samples were taken for the determination of VCAM-1 and IL-6 parameters using Biotin-Streptavidin-Amplified Enzyme-Linked Immuno-sorbent Assay method. Data analysis of VCAM-1 and IL-6 levels between test groups were conducted by means of normality test and One-way ANOVA (p<0.05). Based on the data of VCAM-1 and IL-6 levels, nanocurcumin dose of 80mg/kg BW preparation was able to significantly improve the preventive activity of curcumin compared to curcumin suspension dose of 80mg/kg BW by reducing VCAM-1 levels in SNEDDS (53.260%), nanoemulsion (52.737%), nanosuspension (52.325%) and solid lipid nanoparticles (51.444%) and decreasing IL-6 levels in SNEDDS (33.030%), nanoemulsion (31.568%), nanosuspension (29.898%), and solid lipid nanoparticles (28.875%).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Directorate of Research and Community Service of the Universitas Islam Indonesia, Laboratory of Pharmacology and Laboratory of Pharmaceutical Technology Department of Pharmacy Universitas Islam Indonesia
2019-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1417
10.14499/indonesianjpharm30iss1pp58
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 58
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1417/894
Copyright (c) 2019 Indonesian Journal of Pharmacy
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1144
2017-08-30T06:55:29Z
3:ART
QUALITY OF LIFE ANALYSIS IN DIABETES MELLITUS TYPE 2 PATIENTS USING MONOTHERAPY AND COMBINATION TREATMENT OF MEDICINE
Faridah, Imaniar Noor; Ahmad Dahlan University
Dewintasari, Venty; Ahmad Dahlan University
Diabetes mellitus, DQLCTQ, monotherapy, combination
Treatment of diabetes mellitus (DM) can be vary, using monotherapy or combination therapy, and it depends on the severity of the disease. That variation will give influences to the patient’s clinical condition and also their quality of life (QoL). The objective of this study is to determine the average of QoL’s score in DM patients who use monotherapy and combination therapy of antidiabetic oral in Public Health Center of Kotagede 1 Yogyakarta. This study was conducted in a cross sectional study. Subjects were people who had an age above 18, got the diagnose of DM type 2, and also consumed of oral antidiabetic monotherapy or combination. The measurement of QoL is using a DQLCTQ questionnaire in Bahasa Indonesia. Analysis statistic with independent sample T-test was used to determine the differences between QoL in patients who use monotherapy and combination. Subjects who met the inclusion criteria are 52 patients. About 82.70% (43 patients) of the patients used combination and the other (9 patients) used monotherapy. The average of QoL in monotherapy patients (78.95±11.36) was higher than in combination therapy’s (75.18±10.57). Result of the analysis statistic showed that there is no significant differences (p0.095) of QoL between monotherapy and combination therapy patients.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-06-05
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1144
10.14499/indonesianjpharm28iss2pp119
Indonesian Journal of Pharmacy; Vol 28 No 2, 2017; 119
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1144/809
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/509
2018-09-05T01:49:54Z
3:ART
DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHY AND SPECTROSCOPIC METHODS FOR THE ANALYSIS OF DOXOFYLLINE IN PHARMACEUTICAL DOSAGE FORMS
Thiruvengadam, Ethiraj; Dept of Pharmaceutics &Pharmaceutical Analysis. The Erode College of Pharmacy, Erode, Tamilnadu, 638112, India
Ramadoss, Revathi; Dept of Pharmaceutics &Pharmaceutical Analysis. The Erode College of Pharmacy, Erode, Tamilnadu, 638112, India
Vellaichamy, Ganesan; Dept of Pharmaceutics. The Erode College of Pharmacy, Erode, Tamilnadu, 638112, India
A high performance liquid chromatography (HPLC) and ultraviolet spectroscopic (UV) methods were developed and validated for the quantitative estimation of doxofylline (DF) in pharmaceutical dosage forms. HPLC was carried out using reversephase technique on RP-8 column with a mobile phase composed of 0.05M phosphate buffer pH 6 and acetonitrile (60:40, v/v). The mobile phase was pumped at a flow rate of 1mL/min, and detection was made at 230nm with PDA detector. UV method was performed with λ max at 270nm with apparent molar absorptive of 0.878x103 L mol-1 cm-1. Both the methods showed good linearity, recovery and precision. No spectral or chromatographic interferences from the tablet excipients were found in UV and HPLC methods. The various parameters such as linearity, precision, accuracy, specificity, and robustness, limit of detection and limit of quantization were studied according to ICH guidelines. Statistical analysis was done by student’s t-test and F-test, which showed no ignificant difference between the results of both methods. So the proposed methods could be applicable for routine analysis of DF and monitoring of the quality of marketed drugs. Key words: Doxofylline, Validation, HPLC, UV spectroscopy, Comparisonstudies, Student’s t-test, F-test.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2013-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/509
10.14499/indonesianjpharm24iss1pp14-21
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 14-21
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/509/387
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1439
2019-10-10T01:30:23Z
3:ART
Immunomodulatory Activity of Yogurt Fortified with Honey and Rosella (Hibiscus sabdariffa L.) on Reactive Oxygen Intermediate (ROI) and Nitric Oxide (NO) Secretion
Mahfudh, Nurkhasanah; Faculty of Pharmacy, Universitas Ahmad Dahlan
Novitasari, Rina; Faculty of Pharmacy, Universitas Ahmad Dahlan
Immunomodulator, Yoghurt, Rosella (Hibiscus sabdariffa L), ROI, NO
Raise the body's immune system. Rosella (Hibiscus sabdariffa Linn.) is known to have anthocyanin compounds that have antioxidant and immunomodulatory effects. The aim of this research is to know the effect of immunomodulator yogurt which fortified by rosella (Hibiscus sabdariffa L.) extract to increase the secretion of Reactive Oxygen Intermediate (ROI) and Nitric Oxide (NO). This research was conducted in vivo using 25 male mices with Balb/C strain divided into 5 groups: normal group, plain yogurt treated group and 2%, 4%, 8% rosella yogurt treated group. Treatment was given for 21 days orally. On the 22nd day the mice were sacrificed by taking peritoneum macrophage cells and then tested the secretion of Reactive Oxygen Intermediate (ROI) and Nitrite Oxide (NO). The results showed that there was an increase of ROI and NO secretion in 2%, 4%, and 8% rosella yoghurt treated groups compare to plain yoghurt. The fortification of yoghurt with rosella extract and honey increase the potency of yoghurt in increasing the immunomodulatory activity
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-05-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1439
10.14499/indonesianjpharm30iss2pp141-146
Indonesian Journal of Pharmacy; Vol 30 No 2, 2019; 141-146
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1439/901
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1105
2018-01-09T08:38:31Z
3:ART
Antiinflamatory and antidepressive activities of Extract Curcuma xanthorrhiza Roxb in Systemic Lupus Erythematosus
Setiawati, Maria Caecilia; STIFAR Yayasan pharmasi Semarang
Ikawati, Zulies
Kertia, I Nyoman
Systemic Lupus Erythemathosus is an autoimmune, inflammatory, chronic disorder characterized by multiorgan system involvement. Depression and anxiety are frequent complaints among patients with lupus erythematosus, so antidepressive treatment should be an important element of the therapy of patients with lupus erythematosus. Research into curcumin’s potential as a treatment for depression is still in its infancy, although several potential antidepressant mechanisms of action have been identified. 4-weeks double-blind, placebo-controlled clinical trial was conducted in 14 SLE patients (10 as treatment group and 4 as control group) The BDI (Beck Depression Inventory) score was calculated and TNF α concentration was measured in the serum subjects before and after treatment. Correlation between TNF α concentration and BDI was assessed. After 4 weeks treatment, the TNF α concentration from subjects in treatment group were significantly lower than before ( p <0.001) but the BDI score were not significantly lower than before (p = 0.059). The TNF α concentration and BDI score showed positive correlation, p = 0,024. This study demonstrate that Curcuma xanthorrhiza Roxb extract can decrease serum TNF α concentration and reduce clinically symptoms of depression in SLE patients
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-08-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1105
10.14499/indonesianjpharm28iss3pp185
Indonesian Journal of Pharmacy; Vol 28 No 3, 2017; 185
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1105/836
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1020
2017-08-25T03:51:50Z
3:ART
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1064
2017-09-11T06:10:22Z
3:ART
SYNTHESIS AND ANALGETIC ACTIVITY EVALUATION OF 4-[N-(4-HYDROXYPHENYL)CARBOXYMIDOYL]-2-METHOXYPHENOL
Pudjono, Pudjono; Department of Pharmaceutical Chemistry. Faculty of Pharmacy, Universitas Gadjah Mada
Yogyakarta 55281
Anindita, Jessica
Hakim, Arief Rahman
Purnomo, Hari
Paracetamol is an analgesic-antipyretic compound derived from p-aminophenol. Though paracetamol has good efficacy and safety on consumption, parasetamol has hepatotoxic effect as its adverse drug reaction. 4-[N-(4-hydroxyphenyl)carboxymidoyl]-2-methoxyphenol is one of p-aminophenol derivative that was already been determined in silico using molecular docking PLANTS method, and it was known that 4-[N-(4-hydroxyphenyl) carboxymidoyl]-2-methoxyphenol has analgesic effects more potent and has hepatotoxic adverse effect lower than paracetamol. 4-[N-(4-hydroxyphenyl)carboxymidoyl]-2-methoxy-phenol can be synthesized through reaction of p-aminophenol with vanillin under acid condition. The synthesized products were recrytalized, dried, and the purity was determined with melting point determination and Thin Layer Chromatography. The structure of pure crystals were elucidated using IR, 1H-NMR, C-NMR, and Mass Spectroscopy. The analgesic evaluation was carried in vivo using writhing test method. The synthesized compound were divided into three dosage variations, 0,5; 1; and 2 mol equivalent to 100 mg/kgBB of paracetamol (reference drug). 4-[N-(4-hydroxyphenyl)carboxymidoyl]-2-methoxyphenol with 1 mol dosage has analgesic activity better than paracetamol but the difference was not significant.Keywords: 4-[N-(4-hydroxyphenyl)carboxymidoyl]-2-methoxyphenol, p-aminophenol, analgesic, writhing test
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-03-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1064
10.14499/indonesianjpharm27iss2pp99
Indonesian Journal of Pharmacy; Vol 27 No 2, 2016; 99
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1064/775
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1259
2018-10-23T02:18:57Z
3:ART
Development And Validation Of Stability Indicating RP-HPLC Method For Determination Of Ceritinib
Adhao, Vaibhav Suresh; Dr. Rajendra Gode College of Pharmacy, Malkapur.
Sharma, J; Dr. B.R. Ambedkar University, Uttar Pradesh, India
Thakre, M; Dr. B.R. Ambedkar University, Uttar Pradesh, India
Stability-indicating, HPLC, Ceritinib, Validation, Stress Testing
A new, simple, specific, accurate and precise RP-HPLC method was developed for determination of Ceritinib. In the present study, stress testing of Ceritinib was carried out according to ICH guidelines Q1A (R2). Ceritinib was subjected to stress conditions of hydrolysis, oxidation, photolysis and neutral decomposition. Extensive degradation was found to occur in acidic, condition. Mild degradation was observed in basic and at thermal conditions. Successful separation of drug from degradation products formed under stress conditions was achieved on a Hypersil BDS C18 column (250 mm × 4.6 mm, 5.0 μ particle size) using acetonitrile: acetate buffer (pH 3.7 ± 0.05) (50:50 v/v), at a flow rate of 1.0 mL/min and column was maintained at 40˚C. Quantification and linearity was achieved at 272 nm over the concentration range of 5 - 100 μg/mL for Ceritinib. The method was validated for specificity, linearity, accuracy, precision, LOD, LOQ and robustness.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-01-09
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1259
10.14499/indonesianjpharm28iss4pp241
Indonesian Journal of Pharmacy; Vol 28 No 4, 2017; 241
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1259/857
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/668
2020-01-27T02:51:49Z
3:ART
IN VIVO EVALUATION OF MISOPROSTOL FLOATING MICROSPHERES
Vanitha, Kondi; Vishnu institute of pharmaceutical
education and research, Medak, A.P., India
Varma, Mohan; Shri Vishnu college of pharmacy,
Bhimavaram, A.P.,India
Ramesh, Allure; Vishnu institute of pharmaceutical
education and research, Medak, A.P., India
Prostaglandin (PG) has been reported to be an important protective and acid suppressive factor in the gastric mucosa. The objective of the study was to develop and evaluate a stomach specific drug delivery system for controlled release of Misoprostal a PE analogue for gastric ulcer induced NSAIDs. Floating microspheres were prepared by emulsification-solvent evaporation method using ethyl cellulose as a polymer, carbopol as mucoadhesive polymer. Ulcers were induced by the oral administration of absolute ethanol (5 mL/kg) to 24 h fasted Wistar male rats (n=8), weighing 200 g. Sodium bicarbonate solution, misoprostal solution and drug loaded microspheres were tested. Formulations were administered orally 1h before the administration of ethanol. Prior to the oral administration, rats were anesthetized with ethylic ether. After 2 h of ethanol administration, animals were sacrificed; the stomachs were removed, opened along he greater curvature and examined for lesion measurements. Ulcer indexes (UI) were calculated. The in vivo evaluation showed that ulcer index values were 0.61 ± 0.14 for the sodium bicarbonate solution, 0.58 ±0.18 for the misoprostal and 0.11 ± 0.06 for the misoprostal-loaded microspheres. The KruskalWallis test detected statistical differences (p =0.002) between the ulcer indexes. The multiple analyses (Student-Newman-Keuls) showed that the misoprostal loaded microspheres presented a gastric ulcer index statistically lower than that of the sodium bicarbonate solution (p = 0.001) and the misoprostal solution (p = 0.021). The percentages of ulceration inhibition were 31 and 75% after the administration of misoprostal solution and microspheres, respectively. In conclusion, the in vivo evaluation showed that the microspheres presented ulcer index lower than the solutions, showing that misoprostal-loaded microspheres were efficient in protecting the stomach against ulcer formation.Key words: Misoprostal, microspheres, ulcer index,
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/668
10.14499/indonesianjpharm23iss2pp99-103
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 99-103
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/668/534
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1492
2022-10-26T01:05:53Z
3:ART
Quantitative Analysis of Multi-components in Curcuma xanthorrhiza by Single Marker
Badrunanto, Badrunanto
Wahyuni, Wulan Tri
Nirwati, Hera
Rafi, Mohamad
C. xanthorrhiza; curcuminoids; QAMS; relative coefficient factor
A new simple and effective routine analytical method for quantification of curcuminoids in Curcuma xanthorrhiza was developed by high-performance liquid chromatography. This method based on chromatographic fingerprint combined with a quantitative analysis of multi-components by single marker (QAMS). Curcumin was selected as an internal marker for the determination of two other similar compounds, i.e. bisdemethoxycurcumin and demethoxycurcumin, by using the relative coefficient factor (RCF). Excellent linearity was obtained for each component (r2 > 0.9998), and the recovery of extraction methods were within 100.23-103.95%. The precision of the method was good at inter-day and intra-day analysis (RSD < 4.0%). The stability of RCFs was good under various chromatographic conditions with RSD < 1%, and the ratio of retention time was used to locate each compound. The quantification of curcuminoids between QAMS and external standard method (ESM) proved the consistency and similarity of the two method (RSD < 2%). This study demonstrated that QAMS could be used as a routine method for quality control of curcuminoids in C. xanthorrhiza. This method successfully proved accurate, stable, more effective and simple than external standard method.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-10-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1492
10.14499/indonesianjpharm30iss4pp301
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 301
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1492/921
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1159
2017-09-11T06:08:09Z
3:ART
NOVEL CHRONOTHERAPEUTIC MULTIPARTICULATE DRUG DELIVERY SYSTEM OF FELODIPINE: AN EFFECTIVE TREATMENT FOR CARDIAC ARRHYTHMIA
Banerjee, Sabyasachi; CPS Formulations, Dr. Reddy’s Laboratories Ltd, Hyderabad
Shankar, K Ravi; Sri Sai Aditya Institute of Pharmaceutical Sciences and Research
Rajendra, Y Prasad; College of Pharmaceutical Sciences, Andhra University
hot-melt; central composite design; pharmacokinetics; arrhythmia; extrudates
Arrhythmia follows chronobiology, thus necessitating development of time-dependent formulation for its treatment. The aim of the current work was to develop a solubility-enhanced chronotherapeutic system of felodipine, a widely prescribed anti-arrhythmic. Systematically optimized hot-melt extrusion process was employed to formulate solubility-enhanced extrudates. Film-casting method was adopted for the selection of polymers. Drug released at 5, 15, 30min was taken as response variables in 32 face-centered cube design. Nearly 10-fold increase was observed in the solubility of the optimized extrudates in comparison to pure drug. Physical characterization of the extrudates depicted complete amorphization of drug. Sequential coating was performed on to the extrudates to enable a time-dependent release. In-vitro studies clearly demonstrated that 25% of drug was available rapidly within 10 min of administration. The remaining 75% of drug was available over a period of 4, 8 and 12h. Stability studies performed for 6 months at accelerated conditions depicted no significant change in the physicochemical characteristics of the optimized formulation. In-vivo pharmacokinetic studies conducted in beagle dogs ratified the results of in-vitro studies where a sequential time dependent absorption of felodipine was observed over a period of 12h. Concisely, the studies demonstrated successful development of solubility-enhanced chronotherapeutic system of felodipine.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-03-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1159
10.14499/indonesianjpharm28iss1pp42
Indonesian Journal of Pharmacy; Vol 28 No 1, 2017; 42
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1159/803
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/813
2017-09-13T03:22:07Z
3:ART
OVERPRODUCTION OF MERCURIC REDUCTASE FROM MERCURY-RESISTANT BACTERIA KLEBSIELLA PNEUMONIAE ISOLATE A1.1.1
Fatimawali, Fatimawali; Medical Faculty, Sam Ratulangi University Manado, Jl. Kampus Unsrat Kleak Manado 95115
Kepel, Billy; Medical Faculty, Sam Ratulangi University Manado, Jl. Kampus Unsrat Kleak Manado 95115
Tallei, Trina Ekawati; Faculty of Mathematics and Natural Sciences, Sam Ratulangi University Manado; Jl. Kampus Unsrat Kleak Manado 95115.
Mercury is a highly toxic compound in human. It can, however, be detoxified by mercuric reductase (MerA) protein derived from mercury resistant bacteria. This study aims to obtaine MerA protein by transforming merA gene into Escherichia coli BL21. Nucleotide sequence of merA gene of mercury resistant bacteria Klebsiella pneumoniae isolates A111, optimized by using gene program designers (www.dna20/com) then commercially synthesized and cloned in pET32b expression plasmid vector. Plasmid was transformed into Escherichia coli BL21 to produce MerA protein recombinant, induced with isopropyl-β-D-thiogalactopyranoside (IPTG). MerA proteins were analyzed by 10% sodium dodecyl sulphate poly acrylamide gel electrophoresis (SDS PAGE). The result showed that MerA protein with 60kDa was detected on SDS PAGE. The obtained MerA protein can be used in further research for the enzymatic detoxification of inorganic mercury.Key words: mercuric reductase, merA gene, MerA protein, Escherichia coli BL21
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2015-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/813
10.14499/indonesianjpharm25iss3pp141
Indonesian Journal of Pharmacy; Vol 26 No 3, 2015; 141
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/813/748
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1339
2018-11-02T06:46:53Z
3:ART
Physical and Chemical Properties of Native and Fully Pregelatinized Cassava Starch (Manihot esculenta Crantz)
Dewantara Putra, I Gusti Ngurah Agung; Udayana University
Murwanti, Retno; Universitas Gadjah Mada
Rohman, Abdul; Universitas Gadjah Mada
Sulaiman, T.N Saifullah; Universitas Gadjah Mada
cassava starch fully pregelatinized, native cassava starch, physical properties, chemical properties, amylose, amylopectin
Starch is widely used as an excipient in pharmaceutical formulations because it is inert and it can be mixed with drugs without any chemical reactions. This study was aimed to develop and to characterize the physical and chemical properties of cassava starch fully pregelatinized (CSFP) and native cassava starch (Manihot esculenta Crantz) (NCS). Organoleptic properties, pH, ash content, shrink drying, macroscopic and microscopic analyses, amylose and amylopectin content, bulk and tapped density, the angle of repose and flow rate were physically evaluated for both type of cassava starch. Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), energy-dispersive x-ray spectroscopy (EDS), and differential scanning calorimetry (DSC) were used to characterize and evaluate the chemical properties of the CSFP and NCS. The results of this study indicate that CSFP exhibited different values of those determined parameters compared to that of NCS organoleptic properties i.e, pH, viscosity, ash content, shrink drying, macroscopic and microscopic analyses, amylose and amylopectin content, bulk and tapped density, angle of repose and flow rate. The measurement results with DSC obtained Tg at NCS of 68.18oC while in CSFP there is no Tg because cassava starch (CS) is fully gelatinized. In conclusion, CSFP as a good profile starch contained a higher amount of amylose with larger particle size and good particle density and viscosity than the natural starch and improve its flow properties and compactibility. CSFP had a noticeable effect on fragility, hardness, disintegration time and percentage of drug release from the tablets produced, that can be developed as a pharmaceutical excipient in development of solid dosage forms (sustain release).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
LPDP BUDI
UPT Forensik Udayana University
2018-09-28
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1339
10.14499/indonesianjpharm29iss3pp145
Indonesian Journal of Pharmacy; Vol 29 No 3, 2018; 145
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1339/876
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/685
2020-01-27T02:53:31Z
3:ART
ENHANCEMENT OF DISSOLUTION RATE OF MODAFINIL USING SOLID DISPERSIONS WITH POLYETHYLENEGLYCOLS
Jigar, Vyas; Department of Pharmacy, Bhagwant University, Sikar Road, Ajmer, Rajasthan, India. 305004
Jayvadan, Patel; Nootan Pharmacy College, Visnagar, Gujarat, India. 384315
A., Jain D.; Department of Pharmacy, Bhagwant University, Sikar Road, Ajmer, Rajasthan, India. 305004
Solid dispersions (SDs) of modafinil (MDF) were prepared using polyethyleneglycols (PEGs), in 1;1, 1;2 and 1;4 proportions by fusion, solvent evaporation and physical mixing method. Differential scanning calorimetry (DSC) and X-ray powder diffractometry (XRD) were used to examine the physical state of the drug. The data from the XRD showed that the drug was converted to amorphous form as the number and intensity of peaks were decreased in solid dispersion as compared to pure drug and physical mixture of drug and carrier. DSC thermograms also confirmed the change in physical state of the drug as the peaks were altered or disappeared. With the highest ratio of the carriers (1:4), the drug solubility was enhanced by 38.68, 34.78 and 9.29 folds in solvent evaporation, fusion and physical mixing methods respectively. Solid dispersion batch S6 containing drug:PEG6000 in 1:4, was selected to be formulated as tablet (batch TS6) and evaluated for in vitro drug dissolution & six month stability. An increased dissolution rate of modafinil was observed from SDs and PMs, as compared to pure crystalline drug. The dissolution rate of modafinil from its PMs or SDs increased with an increasing amount of polymer.Key words: Fusion, solvent evaporation, physical mixture, in vitro dissolution, characterization.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/685
10.14499/indonesianjpharm23iss4pp209-215
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 209-215
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/685/551
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1691
2021-01-06T13:13:53Z
3:ART
An Assessment of Cough Medicine Dispensing Practice to Children Under Two Years Old in Pharmacies in Ho Chi Minh City Using Simulated-Patient Method
Phan, Quyen Thi Ngoc; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Truong, Van Thi; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Pham, Thanh Ngoc; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Nguyen, Ho Nhu; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Department of Pharmacy, Nguyen Trai Hospital, Vietnam
Bui, Quynh Thi Huong; Department of Clinical Pharmacy, Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam
Department of Pharmacy, Thong Nhat Hospital, Vietnam
Cough medications, Children, Pharmacy
Over-the-counter (OTC) cough and cold medications (CCMs) have been used to treat the symptoms of upper respiratory infection in children for decades. The safety of CCMs in children has been questioned. The data on knowledge of pharmacists in supplying cough medicines for children under two years have been limited. This study aimed to evaluate the pharmacists’ dispensing decisions to manage the cough in children under two years old. A descriptive cross-sectional was carried out in 300 pharmacies in 15 districts in Ho Chi Minh City, Vietnam. The pharmacists were interviewed by a simulated patient. The results showed that, information that pharmacists actively asked the client about the patient and disease symptoms was limited. Most pharmacists did not provide adequate instructions and counsel about using drugs for clients. Only 22/300 (7.33%) of pharmacists appropriately provided cough medicines for children under 2 years old. The main reason of inappropriateness was the deficiency of knowledge about updated contraindication of N-acetylcysteine (93.17%). Pharmacists in pharmacies located in districts 3, 11 and Binh Thanh had higher rate of rational provision than those in other districts. A good and full understanding of the patient symptom helped the pharmacists supply cough medicines more reasonably. The limited caution of pharmacists and the low proportion of pharmacists updating contraindication of N-acetylcysteine should be considered as a warning sign in pharmacy practice in Ho Chi Minh City, Vietnam.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2020-04-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1691
10.14499/indonesianjpharm31iss1pp42
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 42
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1691/943
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1691/265
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/793
2017-09-11T04:52:17Z
3:ART
Design and evaluation of guargum - based timolol maleate ocular insert For the treatment of glaucoma
Thakral, Sunil; Lachoo Memorial College of Science and Technology, Pharmacy Wing, Jodhpur – 342 003, Rajasthan, India
Purpose: To prepare ocular inserts of timolol maleate using guar gum as a polymer for treatment of glaucoma. Methods: Timolol maleate ocular inserts were prepared by solvent casting method using guar gum in different proportions (0.25% w/v, 0.50% w/v , 75 % w/v and 1.0% w/v). The prepared formulations were evaluated for thickness, weight variation, percentage drug content, surface pH, folding endurance, percentage moisture absorption and loss, percentage swelling, mechanical strength and in vitro transcorneal permeation. In-vitro transcorneal permeation study was performed on goat cornea using modified Franz diffusion cell. Results: The inserts were found to be of uniform thickness (ranging from 41.12±0.04µm to 79.90±0.03µm) and weight (0.84±0.07 mg to 2.11±0.09 mg). The % drug content in the inserts was found to be varied between 98.69±0.58to 96.37±0.58. The cumulative % drug releases from the formulation ranged from 50.22±1.41 to 97.72±0.67over a period of 24 h. In-vitro transcorneal study revealed that an increase in concentration of polymer slows down the release of timolol maleate from the formulation. Conclusion: Ocular inserts using guar gum as a polymer were successfully prepared and can be effectively used for sustain ocular delivery over a period of 24 h.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-08-03
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/793
10.14499/indonesianjpharm26iss4pp177
Indonesian Journal of Pharmacy; Vol 26 No 4, 2015; 177
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/793/807
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/443
2020-02-19T02:37:07Z
3:ART
AND DOSAGE RANGE TESTS OF TENSIGARD AS A HYPOTENSIVE PHYTOPHARMACA
Djatmiko, M.; PT. Phapros Tbk Semarang
Suhardjono, Djoko; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
Nugroho, Agung Endro; Faculty of Pharmacy
Universitas Gadjah Mada
Yogyakarta
Tensigard is a phytofarmaca product of Agromed ( PT. Phapros Tbk., Semarang) formulated for antihypertension therapy. The product comprises celery extract (Apium graveolens) 75 % and kumis kucing extract (Orthosiphon stamineus Benth) 25 %. The aims of the research is to study whether Tensigard has an effect to decrease blood pressure in the normo and hypertensive (adrenaline induced hypertension) experimental animals. The specific aim of the study is to determine the D50 value of Tensigard to lower the blood pressure of the hypertensive experimental animals. The tests were conducted using anesthetized using anesthetized cats which were randomly divided into two groups, each of which consisted of 35 normotensive cats and the other 35 cats with hypertension (due to adrenaline treatment, in which the blood pressure was increased about 1,5 times than normal value). Furthemore, each group was devided into seven sub groups, each of which consists of 5 cats. One sub group was used as the control group, while the remaining sub groups were treated with Tensigard (6 dosage levels). The results of the studies indicated, that Tensigard has a hypotensive effect in the cats,either with normal or hypertension (adrenaline induced). The D50 value of the hypotensive effect in hypertensive cats is 16,37 1,08 mg/kg BW., in which the extrapolation of this dosage value for a 50 kg, human being is about 249,05 mg.Key Word : tensigard, antihypertension, effective dosage
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/443
10.14499/indonesianjpharm0iss0pp33-43
Indonesian Journal of Pharmacy; Vol 12 No 1, 2001; 33-43
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/443/322
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1512
2019-10-10T01:29:33Z
3:ART
Conjugation of Anti-EpCAM Antibody on Alginate–RIP MJ-30 Nanoparticle through Carbodiimide Reaction as a Model of Targeted Protein Therapy
Ismail, Hilda; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia – 55284
Ciptasari, Ummi H.; Undergraduate Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia - 55284
Ikhsan, M Arief Nur; Undergraduate Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia - 55284
Suryani, Fidya; Undergraduate Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia - 55284
Sismindari, Sismindari; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia – 55284
Martien, Ronny; Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia – 55284
Yuswanto, Ag; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia – 55284
Ribosome inactivating proteins from Mirabilis jalapa L. (RIP MJ) has shown higher cytotoxic activity when being formulated as a nanoparticle. However, the selectivity of the delivery system is also an important aspect when it comes to cytotoxic cell therapy. Epithelial cell adhesion molecule (EpCAM) is a monomeric glycoprotein which is overexpressed in epithelial cancer cells. This study aim was to develop a model of targeted protein delivery system by formulating the base fraction of RIP MJ (RIP MJ-30) into alginate nanoparticles and conjugating it with anti-EpCAM antibody. RIP MJ-30 was formulated in to nanoparticle using alginate and CaCl2 as cross-linker. Optimization of conjugation reaction condition was done in the pH variation of 4.5, 5.5, and 6.5. The success of conjugation was analyzed qualitatively using native polyacrylamide gel electrophoresis (native-PAGE) method and BCA assay. The optimum formula of RIP MJ-30 nanoparticles was produced using 0.3% alginate and 0.2% CaCl2. Results indicated that optimum conjugation reaction was carried out at pH level of 5.5. The optimum native-PAGE condition was by using 8% polyacrylamide gel in duration of 6h. Characterization of nanoparticle resulted in particle size of 205.0nm, zeta potential of -6.9mV, entrapment efficiency of 71.11±4.84%, and conjugation efficiency of 89.55±6.18%. It was concluded that RIP MJ-30 was successfully formulated into alginate nanoparticle and conjugated to anti-EpCAM antibody through carbodiimide reaction using 1-ethyl-(dimethylprophilamine) carbodiimide (EDAC).
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-03-30
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1512
10.14499/indonesianjpharm30iss1pp52
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 52
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1512/893
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1185
2018-01-09T08:39:01Z
3:ART
FORMULATION AND EVALUATION OF ORAL SUSTAINED IN SITU GELLING SYSTEM OF ROXATIDINE
Ahmed, Mohammed Gulzar; Professor & Principal
Yenepoya Pharmacy College & Research Centre
Yenepoya University
Mangalore 575018
Karnataka
Kapoor, Chirag
A, Sanjana
Gel dosage forms are successfully used as drug delivery systems to control drug release and protect the medicaments from a hostile environment. The main objective of this present work is to formulate and evaluate in situ gels of roxatidine for the treatment of peptic ulcer. This system utilizes polymers that exhibit sol-to-gel phase transition due to change in specific physico-chemical parameters. In the present work in situ gels have been developed by using gellan gum and sodium alginate based on the concept of ion activated systems. Sol-to-gel transformation occurred in the presence of monovalent/divalent cations. Formulations were evaluated for clarity, drug content, in vitro gelling capacity, determination of pH, in situ release study, viscosity, gel strength, ex vivo gelation and stability study. All the results found to be satisfactory. Experimental part showed that viscosity of sols and gel strength was increased with increase in the concentration of polymers, also drug release gets sustaining. The formulations were therapeutically efficacious, sterile and provided sustained release of the drug over a period of time. These results demonstrated that the developed system is an alternative to conventional drug delivery systems and can improve patient compliance.Key words: In situ gels, Roxatidine, Peptic ulcer.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
nil
2017-08-03
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1185
10.14499/indonesianjpharm28iss3pp178
Indonesian Journal of Pharmacy; Vol 28 No 3, 2017; 178
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1185/835
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/946
2017-08-03T01:55:59Z
3:ART
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1033
2017-09-11T05:03:46Z
3:ART
FORMULATION DEVELOPMENT AND CHARACTERIZATION OF TEA TREE OIL LOADED ETHOSOMES
Venugopal, Vijayan; Faculty of Pharmacy, AIMST University, Kedah, Semeling, Malaysia-08100.
Tea Tree Oil; Ethosomes; HPTLC; Globule Size; Zeta Potential
To prepare ethosomes containing tea tree oil by hot homogenization method and to evaluate its physical characters and in-vitro release pattern. the preformulation studies were carried out by standard procedure. The morphology of globule was studied by optical microscopy. The globule size and zeta potential was determined by Zetasizer, respectively and in-vitro study was done by diffusion method and the drug content was analyzed by HPTLC method. The release kinetics was also studied by fitting into few mathematical models. All the formulations were showed spherical and unilamellar shape with globule size of 931 to 975 nm, the zeta potential in the range of – 40 to -52 mV and entrapment efficiency was 57 to 65 %. Finally the invitro release studies showed the drug release from the ethosomal vesicles was burst release at initial time followed by sustained release over throughout the study. From the above consideration of evaluation studies, the tea tree oil loaded ethosomal formulation F5 shows best globule size, zeta potential and entrapment efficiency. The sustained action was confirmed by invitro release studies. All the formulations are followed zero order drug release and diffusion type of mechanism of drug releases with Fickian model. Ethosome loaded tea tree oil could be the best choice for topical application.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1033
10.14499/indonesianjpharm27iss1pp44
Indonesian Journal of Pharmacy; Vol 27 No 1, 2016; 44
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1033/767
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1090
2017-09-11T06:17:05Z
3:ART
INFLUENCE OF POMEGRANATE JUICE ON THE CYP3A4-MEDIATED METABOLISM AND P-GLYCOPROTEIN MEDIATED TRANSPORT OF SAQUINAVIR IN VIVO AND EX VIVO MODELS
Vemulapalli, Sridhar; Department of Pharmacology, KVSR Siddhartha College of Pharmaceutical Sciences
CYP3A4; P-glycoprotein; Everted gut sacs; Pomegranate juice; Pharmacokinetics
Cytochrome P450 3A4 (CYP3A4) and P-glycoprotein (P-gp) play an important role in the first pass metabolism thereby limits the oral bioavailability of many clinically important and frequently prescribed drugs. The absolute oral bioavailability of saquinavir is very low (i. e. 4%) due to its extensive first pass metabolism by the major metabolizing isozyme CYP3A4 and it is also a substrate of P-gp. Pomegranate juice (PGJ) was known to be a modulator of CYP3A4 and P-gp. Therefore, the aim of this study was to evaluate the influence of PGJ on the pharmacokinetics (PK) of saquinavir in wistar rats and on the P-gp mediated intestinal transport of saquinavir in everted gut sacs ex vivo. Rats were treated orally with saquinavir (100 mg/kg) alone and in combination with PGJ (0.5, 1.0 and 2.0 mL/200g, body weight) for 15 consecutive days. Blood samples were collected on 1st day in single dose pharmacokinetic study (SDS) and on 15th day in multiple dose pharmacokinetic study (MDS). The peak plasma concentration (Cmax)and area under the plasma concentration-time curve (AUC0-24) of saquinavir was increased with PGJ in SDS (p<0.001) may be due to inhibition of CYP3A4 and P-gp. But interestingly, the Cmax and AUC0-24 of saquinavir was decreased significantly with PGJ in MDS. This is may be due to induction of CYP3A4. The transport of saquinavir was increased in presence of PGJ and known P-gp inhibitors (Verapamil, Ketoconazole and Quinindine) across the rat everted gut sacs ex vivo. The present study results suggested that PGJ has both effects (inhibition, in SDS and induction, in MDS) on CYP3A4-mediated saquinavir metabolism in vivo and inhibitory effect on the P-gp mediated intestinal transport of saquinavir ex vivo. Further studies are needed to confirm this interaction at cellular level using cell lines and in humans.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1090
10.14499/indonesianjpharm27iss3pp152
Indonesian Journal of Pharmacy; Vol 27 No 3, 2016; 152
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1090/785
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/663
2020-01-27T02:53:58Z
3:ART
CHROMOSOME CHARACTERIZATION OF THREE VARIETIES OF GINGER (Zingiber officinaleRosc.)
Daryono, Budi Setiadi; Genetics Laboratory, Faculty of Biology
Universitas Gadjah Mada Jl. Teknika Selatan-Sekip Utara Yogyakarta 55281
Rahma, Siti Nur Azizah Fauziati; Genetics Laboratory, Faculty of Biology
Universitas Gadjah Mada Jl. Teknika Selatan-Sekip Utara Yogyakarta 55281
., Purnomo; Laboratory of Plant Systematics, Faculty of
Biology, Universitas Gadjah Mada. Jl. Teknika
Selatan-Sekip Utara Yogyakarta 55281
., Sudarsono; Departement of Pharmaceutical Biology,
Faculty of Pharmacy, Universitas Gadjah Mada,
Sekip, Utara Yogyakarta 55281, Indonesia
Giant ginger (Zingiber officinale Rosc. var. officinale), red ginger (Zingiber officinale Rosc. var. rubra) and small ginger (Zingiber officinale Rosc. var. amarum) are three varieties of Zingiber officinale Rosc. They have a lot of benefit and often used by Indonesian as a traditional drug. Moreover, they have a big chance to be use as a flavor in world wide. Therefore, research for their quality, quantity and continuity of supplyare needed. Characterization of their chromosomes is one effort for improving ginger cultivation. The objective of this research was to study mitotic time and chromosome characters of three varieties of ginger. Squashing method was used for chromosome preparation. The results showed that mitotic time of giant ginger is 09.00-10.05 am, red ginger is 09.00-10.30 am, while small ginger is 08.45-11.00 am. Chromosome number of giant ginger and small ginger are 2n=2x=30, while red ginger is 2n=2x=22. Giant ginger has R= 3,109, Red ginger has R = 3,206 and small ginger has R = 4,065. Based on chromosome characters it is revealed that relationship between giant ginger and red ginger is closer that of compare to small ginger. This result is important as basic information for improving the gingers production through breeding program.Key words: Zingiber officinale Rosc., mitotic time, chromosome characterization, squashmethod
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/663
10.14499/indonesianjpharm23iss1pp54-59
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 54-59
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/663/529
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1545
2019-10-28T01:20:02Z
3:ART
Effect of 7-Hydroxy-2-(4- Hydroxy -3-Methoxyphenyl)-Chroman-4-one On Level of Mangan-Superoxide Dismutase (Mn-Sod) and Superoxide Dismutase 2 (Sod2) Gene Expression in Hyperlipidemia Rats
Ayunda, Rahmah Dara; Magister of Biomedical Science Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada
Prasetyastuti, Prasetyastuti; Departement of Biochemistry Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada
Hastuti, Pramudji; Department of Biochemistry Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada, Skip Utara 55281 Yogyakarta
Hyperlipidemia, flavonoids, Mn-SOD, SOD2 gene
Hyperlipidemia is a lipid metabolism disorder characterized by an increase in serum lipid levels. Hyperlipidemia is a major risk factor for many metabolic syndrome diseases because it triggers oxidative stress. Oxidative stress can be reduced by endogenous antioxidant enzymes triggered by exogenous antioxidant compounds, such as 7-OH-2- (4-OH-3-methoxyphenyl)-chroman-4-one isolated from the seeds of Swietenia macrophylla King. The aims of this study were to investigate the effects of 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one compounds on cholesterol level, LDL level, Mn-SOD levels and SOD2 gene expression of hyperlipidemic rats. Thirty rats (Rattus norvegicus ) were divided into 6 groups, normal group (N), hyperlipidemia group (HL), hyperlipidemia group with simvastatin (P), hyperlipidemic group with 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one with dose 10 (F10), 30 (F30) and 90 (F90) mg/200g body weight (BW). Cholesterol and LDL were analyzed with CHOD-PAP method, Mn-SOD level was analyzed by ELISA method and SOD2 gene expression was analyzed by qPCR method. The decrease in cholesterol and LDL levels were most prevalent in group F90 with dose 90 mg/200g BW of 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one, with average difference each of them was 172.43 mg/dL and 36.12 mg/dL. The rats fed on high-cholesterol diet exhibited a significant elevation in Mn-SOD levels (p<0.05) compared to normal group. The treated animals with 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one has the level of Mn-SOD is significantly lower (p<0.05) compared with hyperlipidemic group. Expression of SOD2 in group F90 has value close to normal group (p> 0.05). 7-OH-2-(4-OH-3-methoxyphenyl)-chroman-4-one with dose of 90 mg/200g BW improved cholesterol levels, LDL levels, Mn-SOD levels and SOD2 gene expression in hyperlipidemic rats.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2019-08-29
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1545
10.14499/indonesianjpharm30iss3pp180
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 180
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1545/915
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1096
2017-09-05T02:37:22Z
3:ART
Ameliorative effects of vitamins C and E on tenofovir/nevirapine-induced hepatorenal oxidative stress in albino rats
Adikwu, Elias; Department of Pharmacology, Faculty of Basic Medical Sciences, University of Port Harcourt, Choba, Rivers State Nigeria
Apiakise, Williams; Department of Community Health Sciences, College of Health Technology, Otuogidi, Bayelsa State, Nigeria
tenofovir; nevirapine; liver; kidney; oxidative stress; antioxidants; rats
Background and Objectives: Oxidative stress has been implicated in xenobiotic-induced hepatorenal toxicity. This research was designed to investigate the effects of vitamins C and E on the baseline and tenofovir/nevirapine-induced liver and kidney levels of superoxide dismutase (SOD), glutathione (GSH), catalase (CAT) and malondialdehyde (MDA) in male albino rats. Materials and Methods: Forty five male albino rats were used for this study. The rats were divided into groups A – I of 5 rats each. Group A (placebo control) and group B (solvent control) were orally treated with water and arachis oil respectively. Groups C – F were orally treated with vitamins C, E, vitamins C and E, and tenofovir/nevirapine respectively. Groups G– I were pretreated with vitamins C, E and vitamins C and E prior to treatment with tenofovir/nevirapine respectively. Rats were sacrificed after treatment for 30 days; liver and kidneys were collected and evaluated for the above parameters. Results: Significant (p<0.05) increases in the baseline levels of liver and kidney SOD, GSH, and CAT with decreases in MDA levels were obtained in vitamins C and E treated rats when compared to control. On the contrary, treatment with tenofovir/nevirapine significantly (p<0.05) decreased liver and kidney SOD, GSH, and CAT levels with increases in MDA levels when compared to control. However, all tenofovir/nevirapine -induced alterations were abrogated in rats pretreated vitamins C and E. Abrogation was most pronounced in rats pretreated with combined doses of vitamins C and E. Conclusion: Observations in the present study could be attributed to the inhibitory effects of vitamins C and E on the oxidative activity of tenofovir/nevirapine in the liver and kidney of treated rats.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-12-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1096
10.14499/indonesianjpharm27iss4pp211
Indonesian Journal of Pharmacy; Vol 27 No 4, 2016; 211
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1096/795
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/810
2017-09-12T07:19:17Z
3:ART
IN VITRO ANTIDIABETIC AND IN VIVO ANTIDIARRHEAL ACTIVITY OF Oncoba Spinosa ROOTS
M, Prasanth Kumar; Faculty of Pharmaceutical Sciences, JNTU, Hyderabad, Telangana. 500049 India
V, Suba; Department of Pharmacology, National Institute of Siddha, Tambaram Sanatorium, Chennai. 600047 India
B, Ramireddy; Director-Formulations, NATCO Pharma, Hyderabad, Telangana.
P, Srinivas Babu; Principal, VignanPharmacy College, Vadlamudi, Guntur, Andhrapradesh. 522510
The different extracts of O.spinosa (Flacourtiaceae) were screened for in-vitro antidiabetic activity. Of all the extracts tested, ethanolic extract showed highest alpha amylase inhibition ranging from 8.64+0.66% to 79.94+0.65% and alpha glucosidase inhibition ranging from 14.12+0.51% to 78.68+0.36% when studied at concentrations 62.5-1000μg/mL. The ethanolic extract was subjected to antidiarrheal activity and diarrheal severity was reduced significantly by 15.81% in 100mg/kg group, 30.45% in 200mg/kg group and 74.37% in 400mg/kg group in castor oil induced diarrhea model. In castor oil induced enteropooling, the extract at doses 200mg/kg and 400mg/kg showed 33.46% and 42.44% inhibition of intestinal accumulation. In the charcoal meal test, the distance travelled by charcoal meal was significantly reduced by the extract at doses 200mg/kg and 400mg/kg (P<0.01). The overall results tend to suggest the antidiabetic and antidiarrheal activities of O. spinosa.Key word : in vivo, in vitro, antidiabetic, antidiarrheal, O.spinosa, roots
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2015-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/810
10.14499/indonesianjpharm25iss3pp121
Indonesian Journal of Pharmacy; Vol 26 No 3, 2015; 121
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/810/751
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
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oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1330
2018-10-23T02:19:33Z
3:ART
Formulation of Ketorolac Tromethamine for Controlled Release in Gastrointestinal and Colonic Delivery System
Hossain, Md. Amzad; Department of Pharmacy, University Science and Technology Chittagong, Bangladesh.
Shill, Swapon Kumar; Professor of Chemistry, University of Chittagong, Bangladesh
Khan, Shakhawat Hasan; Professor of Chemistry, University of Chittagong, Bangladesh
Hossen, S. M. Moazzem; Department of Pharmacy, University of Chittagong, Chittagong-4331, Bangladesh.
Uddin, Mohammad Nasir; Professor of Chemistry, University of Chittagong, Bangladesh
Drug Formulation, Ketorolactromethamine, gastrointestinal and colonic delivery system, HPLC analysis
A suitable matrix system of ketorolac tromethamine (KTR) formulation has been developed with the aim of increasing the contact time, achieving controlled release, reducing the frequency of administration, improving patient compliance. In this concern an enteric-coated KTR matrix tablet intended for specific delivery of drugs to the colon by combining the use of a time dependent core with a pH-sensitive film coating. Eudragit L100, with a threshold pH 7, was selected as coating material. New formulation is proved to be noble as to KTR delivery through both gastrointestinal and colonic system. New formulation is considered to reduce gastrointestinal side effects and achieve high local drug concentration at the afflicted site in the gastro-intestine and colon.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-07-09
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1330
10.14499/indonesianjpharm29iss2pp49
Indonesian Journal of Pharmacy; Vol 29 No 2, 2018; 49
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1330/864
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/680
2020-01-27T02:53:16Z
3:ART
EXPRESSION OF RECOMBINANT HUMAN ERYTHROPOIETIN WITH GLYCOSYLATION MODIFICATION IN HEK293T CELLS
Septisetyani, Endah Puji; Research Center for Biotechnology, Indonesian Institute of Sciences Jl. Raya Bogor KM 46,
Cibinong, Bogor, 16911, Indonesia
Rubiyana, Yana; Research Center for Biotechnology, Indonesian Institute of Sciences Jl. Raya Bogor KM 46,
Cibinong, Bogor, 16911, Indonesia
Wisnuwardhani, Popi Hadi; Research Center for Biotechnology, Indonesian Institute of Sciences Jl. Raya Bogor KM 46,
Cibinong, Bogor, 16911, Indonesia
Wardiana, Andri; Research Center for Biotechnology, Indonesian Institute of Sciences Jl. Raya Bogor KM 46,
Cibinong, Bogor, 16911, Indonesia
Santoso, Adi; Research Center for Biotechnology, Indonesian Institute of Sciences Jl. Raya Bogor KM 46,
Cibinong, Bogor, 16911, Indonesia
Stability of erythropoietin (EPO) depends on its glycosylation states. With more glycosylation sites, the EPO protein will be more stable and also increase its half-life. A construct of recombinant human erythropoietin (rhEPO) which contains 2 additional N-link for glycosylation were designed. Based on translation analysis using ORF (open reading frame)-finder and protein alignment analysis using blast-p of NCBI home page, expected recombinant hEPO with additional 6-histidin tag in carboxyl terminus was expressed. HEK293T cells were transfected with recombinant plasmid containing rhEPO by using calcium phosphate method. Expression of rhEPO was detected by dot blot and Western blot analysis using hEPO antibody as the primary antibody and antirabbit antibody with alkaline phospatase linked as the secondary antibody. The bands were detected by BCIP/NBT color development substrate. The data indicated detection of EPO in culture medium of transfected HEK293T cells.Key words: HEK293T cell, calcium phosphate transfection, N-linked glycosylation, recombinant human erythropoietin
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/680
10.14499/indonesianjpharm23iss3pp177-182
Indonesian Journal of Pharmacy; Vol 23 No 3, 2012; 177-182
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/680/546
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1529
2021-01-06T13:13:53Z
3:ART
Inhibition of Cell Cycle and Induction of Apoptosis y Ethanol Leaves Extract of Chrysanthemum cinerariifolium (Trev.) In T47D Breast Cancer Cells
Mutiah, Roihatul; Department of Pharmacy, Faculty of Medicine and Health Science, Maulana Malik Ibrahim Islamic State University of Malang
Inayatin, Alfiyah Laily; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
Annisa, Rahmi; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
Indrawijaya, Yen Yen Ari; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
Listiyana, Anik; Maulana Malik Ibrahim State Islamic University of Malang Indonesia
C. cinerariifolium, apoptosis, cell cycle, T47D cells
Chrysanthemum cinerariifolium (C.cinerariifolium) is a plant of the Asteraceae family, which has been applied by the community as an ornamental plant and traditional medicine. In this study, the effect of C. cinerariifolium leaves extract on inhibition of cell cycle and induction of apoptosis in T47D breast cancer cells was tested and compared to the standard chemotherapy agent. The citotoxic activity of C. cinerariifolium leaves extract against T47D cancer cells and Vero normal cells was tested by MTT method. Profile of apoptosis and cell cycle were observed by flow cytometry method. Based on chemical compounds profil which is tested used TLC showed that C.cinerariifolium leaves extracts contained flavonoid and terpenoid chemical compounds. The result of cytotoxic test showed that leaves extract of C. cinerariifolium was able to inhibit the growth of T47D cancer cell at IC50 418.8μg/mL. Doxorubicin, extracted from Streptomyces peucetius used as treatment in several cancers including breast cancer. Doxorubicin could inhibit the growth of T47D cancer cells in 115.1μg/mL. The results of cell cycle analysis showed that the C. cinerariifolium leaves extract inhibited cell cycle in G0-G1 and S phase, whereas doxorubicin was able to inhibit cell cycle in G0-G1 phase but experienced cell accumulation in G2-M phase. The percentage of apoptosis in cycle was showed in M1 (sub G1) and M5 (multinuclear) phase which treatment of C. cinerariifolium leaves extract was higher than doxorubicin. Therefore, C. cinerariifolium leaves extract has potential activity as anticancer agent causes inhibition of cell cycle and induction apoptosis.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
This work supported by The Directorate General of Islamic Higher Education (DIKTIS) of Interdisciplinary Basic Research Grant numbers 3209/Un.3/HK.00.5/05/2018.
2020-04-07
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1529
10.14499/indonesianjpharm31iss1pp1
Indonesian Journal of Pharmacy; Vol 31 No 1, 2020; 1
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1529/937
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1424
2019-10-03T03:16:50Z
3:ART
Cytotoxicity of Tetrahydropentagamavunon-0 (THPGV)-0 and Tetrahydropentagamavunon-1 (THPGV-1) in Several Cancer Cell Lines
Ikawati, Muthi; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, 55281, Indonesia
Purwanto, Heri; Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, 55281, Indonesia
Imaniyyati, Niar Nurul; Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, 55281, Indonesia
Afifah, Anis; Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, 55281, Indonesia
Sagiyo, Marrita Langgeng; Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, 55281, Indonesia
Yohanes, Jasson; Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, 55281, Indonesia
Sismindari, Sismindari; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, 55281, Indonesia
Ritmaleni, Ritmaleni; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, 55281, Indonesia
THPGV-0, THGPV-1, curcumin analog, cytotoxic, anticancer
Tetrahydropentagamavunon-0 (THPGV-0) and Tetrahydro-pentagamavunon-1 (THPGV-1), are analogs of a curcumin metabolite, tetrahydrocurcumin, and a derivate of Pentagama-vunon-0 (PGV-0) and Pentagamavunon-1 (PGV-1), respectively. THPGV-0 and THPGV-1 have been successfully synthesized and are investigated for their anticancer potency. Cytotoxic assays were performed toward several cancer cell lines to determine values of the IC50 against those cell lines. Assessing cytotoxicity in Vero normal cell line showed the selectivity of those compound. THPGV-1 showed highest cytotoxic activity in lymphoma Raji cells, a suspension cell line, with an IC50 of 180mM. Both THPGV-0 and THPGV-1 showed similar potencies in T47D breast cancer cell line with IC50 values of 250-270mM. Regardless their high selectivity, however, cytotoxic activities of THPGV-0 and THPGV-1 were lower compared to PGV-0 and PGV-1 in HeLa cervical, T47D breast, and WiDr colon cancer cell lines. Further study using different types of cancer cell lines and confirmation of cell viability by another assays and apoptosis detection may give more benefit.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2018-12-17
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1424
10.14499/indonesianjpharm29iss4pp179
Indonesian Journal of Pharmacy; Vol 29 No 4, 2018; 179
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1424/882
Copyright (c) 2018 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/691
2020-01-27T02:53:31Z
3:ART
SYNTHESIS AND ANALGESIC ACTIVITY OF 6-(MNITROPHENYL)-4-SUSTITUTED BENZYLIDENE-4,5-DIHYDROPYRIDAZIN-3(2H)-ONE DERIVATIVES
Asif, Mohammad; Department of Pharmacy, GRD (P.G) Institute of Management and Technology, Dehradun,
248009, India.
Many research groups have been interested in 3(2H)-pyridazinones for the development of potential analgesic and antiinflammatory agents. Stimulated by these findings, three 6-(mNitrophenyl)-4-sustituted benzylidene-4,5-dihydropyridazin-3(2H)-ones (IVa-IVc) have been synthesized and evaluated for analgesic activity against hot plate method. Compounds IVa-IVc were prepared from 6-m-nitrophenyl-4,5-pyridazin-3(2H)-one by condensation with respective aldehydes. All compounds (IVa-IVc) having nitro phenyl and benzylidene groups at position 6 and 4 of the pyridazinone ring receptively. All compounds (IVa-IVc) showed significant analgesic activity when compare to control group and were found less potent than reference drug aspirin.Key words:Pyridazinones, analgesic, benzylidene, nitrophenyl.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-10-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/691
10.14499/indonesianjpharm23iss4pp254-258
Indonesian Journal of Pharmacy; Vol 23 No 4, 2012; 254-258
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/691/557
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1988
2021-01-07T01:00:39Z
3:ART
In vitro Comparative Study for Anti-proliferative Activity of Some Plant Extracts, Fam. Apiaceae, on HeLa Cell Line
Gomaa, Sara E.; Medicinal and Aromatic Plants Research Dept., Horticulture Research Institute (HRI), Egypt. 9 ALgamaa Street, Giza, Egypt
Friedersdorf, Matthew; Molecular Genetics and Microbiology Dept., Duke University Medical Center, NC, USA
El Enshasy, Hesham Ali; Director,
Institute of Bioproduct Development (IBD), Universit Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Abou-Donia, MB; Pharmacology and Cancer Biology Dept., Duke University School of Medicine, NC, USA.
Apiaceae - Anti-proliferative - Extracts - HeLa Cell Line
In this research, the biological activities of five plant extracts from family Apiaceae; Italian Parsley (Petroselinum neapolitanum), Fennel (Foeniculum vulgare), Celery (Apium graveolens), Cilantro (Coriandrum sativum) and Dill (Anethum graveolens), were studied. Antiproliferative effect of eleven ethanol crude extracts was tested in Human Cervical (Hela) cancer cells. Results clearly demonstrated that all plant extracts showed high significant difference when compared to the negative control (DMSO).Parsley leaves extract, cilantro leaves extract and cilantro stems extract showed no significant difference with the positive control (Actinomycin D). As for, fennel bulb extracts, fennel stalks extracts, celery stems gave better results than the positive control with no significant difference through the 24, 48 and 72 h treatment. There were no significant difference between Fennel extracts and the positive control, which showed high effect on the cancer cells survival. There were no significant difference between both extracts of Cilantro leaves and stems through each time but the best result was after 72 h of treatments. Regarding Dill leaves and stems, cell numbers recorded no significant difference between the both on time dependent manner. Further investigation for ethanolic extracts of parsley leaves, fennel bulb, fennel stalks, celery stems, cilantro leaves and cilantro stems which showed better results than using the commercial drug Actinomycin D (25ml/ml) for 24 h treatment or less depending on concentrations manner. Also, further investigation on different types of cancer cell lines to avoid the toxic effect of chemotherapy.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Jack Kenee, Duke University, USA
2020-08-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1988
10.14499/indonesianjpharm31iss2pp108
Indonesian Journal of Pharmacy; Vol 31 No 2, 2020; 108
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1988/946
Copyright (c) 2020 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1085
2017-09-11T06:09:19Z
3:ART
OPTIMIZING STRUCTURE-BASED VIRTUAL SCREENING PROTOCOL TO IDENTIFY PHYTOCHEMICALS AS CYCLOOXYGENASE-2 INHIBITORS
Istyastono, Enade Perdana; Division of Drug Design and Discovery, Faculty of Pharmacy, Sanata Dharma University
Center for Environmental Studies Sanata Dharma University (CESSDU)
Structure-based virtual screening (SBVS); phytochemical; cyclooxygenase-2 (COX-2)
By employing Databases of Useful Decoys (DUD) and its enhanced version (DUD-E), several attempts to construct validated Structure-based Virtual Screening (SBVS) protocols to identify cyclooxygenase-2 (COX-2) inhibitors have been performed. Both databases tagged active COX-2 inhibitors for compounds with IC50 values < 1mM. In the search for phytochemicals as natural COX-2 inhibitors, however, most of their IC50 values are in the micromolar range, which will likely be identified as non-inhibitors for COX-2 by the available SBVS protocols. In this article, validation of an SBVS protocol by adding marginal active COX-2 inhibitors from DUD-E as active compounds is presented. Binary quantitative-structure activity relationship analysis by using recursive partition and regression tree method was performed subsequently to optimize the predictive ability of the protocol. The enrichment factor and the F-measure values of the optimized protocol could reach 44.78 and 0.47, respectively. The optimized protocol could identify 1 out of 9 phytochemicals as COX-2 inhibitors.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1085
10.14499/indonesianjpharm27iss3pp163
Indonesian Journal of Pharmacy; Vol 27 No 3, 2016; 163
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1085/780
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/515
2018-09-05T01:49:55Z
3:ART
FORMULATION AND EVALUATION OF MUCOADHESIVE BUCCAL TABLET OF DOMPERIDONE MALEATE
Dixit, Yuwaraj Dilipsingh; Sharad Pawar College of Pharmacy, Wanadongri, Hingna Road, Nagpur - 441 110, (MS), India.
Suruse, Pravin Babarao; Sharad Pawar College of Pharmacy, Wanadongri, Hingna Road, Nagpur - 441 110, (MS), India.
Shivhare, Umesh Dhaniram; Sharad Pawar College of Pharmacy, Wanadongri, Hingna Road, Nagpur - 441 110, (MS), India.
The objective of the present study was to develop and evaluate mucoadhesive dosage form of Domperidone maleate. It is an antiemetic synthetic benzimidazole compound that acts as a dopamine D2 receptor antagonist. Mucoadhesive Domperidone maleate tablet formulation was prepared by direct compression method. The formulation F3 containing (Carbopol 940 + sodium alginate) was found to be best among all the formulation batches because of its consistent release rate for 7 h and extent of drug release was 94.44%. Graphical treatment of the formulation F3 to Higuchi’s equation showed that the drug release was diffusion mediated. In-vitro permeability study for formulation F3 for 7 h had shown 76.69% drug release. FTIR studies showed no evidence on interaction between drug and polymers. Key words: Domperidone maleate, Mucoadhesive tablet, antiemetic
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2013-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/515
10.14499/indonesianjpharm24iss1pp47-55
Indonesian Journal of Pharmacy; Vol 24 No 1, 2013; 47-55
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/515/393
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1467
2019-10-28T01:20:02Z
3:ART
Anticancer Molecules from Catharanthus roseus
Taher, Zarani M; Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Agouillal, Farid; 1. Research Unit on Analysis and Technological Development in Environment, URADTE, Centre de Recherche Scientifique et Technique en Analyses Physico-Chimiques, CRAPC, B.P. 384, RP 42004 Zone Industrielle Bou-Ismail, Tipaza, Algeria.
2. Laboratory of Reaction Engineering, LGR, Faculty of Mechanical Engineering and Process Engineering, Houari Boumediene University of Science and Technology, USTHB, PB 32 El Alia, 16075, Bab Ezzouar, Algiers, Algeria.
R, Lim J.; School of Chemical Engineering and Energy, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Marof, Aina Q; Department of Chemistry, Mathematics and Science Faculty, Universitas Negeri Jakarta, Jakarta, Indonesia
Dailin, Daniel Joe; 1. Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
2. School of Chemical Engineering and Energy, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), Johor Bahru, Malaysia.
Nurjayadi, Muktiningsih; Department of Chemistry, Mathematics and Science Faculty, Universitas Negeri Jakarta, Jakarta, Indonesia
Razif, Ezzaty NM; Faculty of Applied Sciences, Universiti Teknologi Mara Negeri Sembilan, Kampus Kuala Pilah, Pekan Parit Tinggi 72000, Kuala Pilah Negeri Sembilan, Malaysia
Gomaa, Sara E; Horticulture Research Institute, Agriculture Research Center, Egypt.
El Enshasy, Hesham Ali; Director
Institute of Bioproduct Development (IBD)
Universiti Teknologi Malaysia (UTM)
Skudai, Johor Bahru, Malaysia
C. roseus; Anticancer; Extraction; Phytochemical; Tissue culture
Catharanthus roseus is an important medicinal plant found in various parts of the world and the bioactive compound has been extracted and used as anti-cancer agent to treat the cancer over decades. However, the extraction of bioactive compound also results in the generation of large quantities of pollution with wasted solvents. Toxic pollution occurs when synthetic chemicals are discharged or natural chemicals accumulate to toxic levels in the environment, causing reductions in wildlife numbers, degrading ecosystem functions and threatening human health. This review covers the extraction and phytochemical obtained leading to chemical compounds related to anti-cancer property of C. roseus. Additionally, recent advances of using biological cell cultures were also addressed. Thus, this work can be used for further investigation of C. roseus to be undertaken in future for its anti-cancer property further development and efficient production in drug industry
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
MOE and UTM-RMC (Malaysia) through HICOE grant no. R.J130000.7846.4J262.
2019-07-23
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1467
10.14499/indonesianjpharm30iss3pp147
Indonesian Journal of Pharmacy; Vol 30 No 3, 2019; 147
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1467/910
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1118
2017-09-11T06:09:06Z
3:ART
SYNTHESIS AND ANTIMICROBIAL EVALUATION OF 2-[(SUBSTITUEDPHENYL)-5-(1-PHENYL-3-(PIPERAZINYL)PYRIDO[3,2-f]QUINAZOLIN-4(1H)-YL]-1,3,4-THIADIAZOLES
Ram, Tilak; Department of Chemistry, Govt. P.G. College, Uttarkashi-249193, U.K., India
Dubey, Ranjana; Department of Chemistry, S.R.M. University, Modinagar-201204, Meerut, U.P., India
Chaudhary, Nidhi; Department of Chemistry, M.I.E.T., Meerut-250001, U.P., India
Several novel 2-[(substitutedphenyl)-5-(1-phenyl-3-(piperazinyl)pyrido[3,2-f] quinazolin-4(1H)-yl]-1,3,4 thiadiazole derivatives have been designed and synthesised by incorporating four known moieties such as 2-methylquinolin-5-ol, acetophenone, urea and 3-substitutedphenyl-4-amino-5-mercapto triazoles by using multi-step conventional reaction strategy. The synthesized derivatives were characterized by IR, 1H-NMR, Mass and elemental analysis (C, H, N). Furthermore the synthesized 2-[(substitutedphenyl)-5-(1-phenyl-3-(piperazinyl)pyrido[3,2-f]quinazolin-4(1H)-yl]-1,3,4-thiadiazoles 4a-g were screened for antibacterial and antifungal activities. The bacterial panel consisted of Staphylococcus aureus, Escherichia coli, Klabsiella pneumoniae, Proteus vulgaris while fungal panel included of Aspergillus fumigatus (plant isolate), Candida glabrata, Candida albacans, Candida krusei. Ampicillin trihydrate and fluconazole were used as reference drugs for antibacterial and antifungal activity respectively. Bacterial and fungal inhibition determined by disk diffusion and serial plate dilution method respectively. Pathogenic inhibitions were determined by measuring the diameter of the inhibition zone in mm. Compound 4c demonstrated significant antibacterial and antifungal spectrum against all the tested microbes.Keywords- antibacterial, antifungal and substituted 1,3,4-thiadiazoles.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2016-09-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1118
10.14499/indonesianjpharm27iss4pp183
Indonesian Journal of Pharmacy; Vol 27 No 4, 2016; 183
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1118/791
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/660
2020-01-27T02:53:58Z
3:ART
ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF RED Psidium guajavaL. LEAVES GROWN IN MANOKO, LEMBANG - INDONESIA
Fidrianny, Irda; Departement of Biology Pharmacy, School of Pharmacy, Intitut Teknologi Bandung
Jl. Ganesha 10 Bandung-40132 Indonesia
Hartanti, Rika; Departement of Biology Pharmacy, School of Pharmacy, Intitut Teknologi Bandung
Jl. Ganesha 10 Bandung-40132 Indonesia
Raveendaran, Narmmatha; Departement of Biology Pharmacy, School of Pharmacy, Intitut Teknologi Bandung
Jl. Ganesha 10 Bandung-40132 Indonesia
Psidium guajava L. (Myrtaceae) is a well known plant in Malaysia and Indonesia. Its leaves extract was found to possess antidiarrhea, antimicrobial, hepatoprotective and antioxidant activities. Objective of this research is to isolate an antioxidant subtance from Red Psidium guajava L. leaves. The crude leaves was extracted using Soxhlet apparatus by gradual polarity of three different solvents, n-hexane, ethyl acetate and methanol. Antioxidant activity of each extract was tested by using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method. Total phenol, total flavonoid and total tannin content of the extracts were also measured. Ethyl acetate extract was fractionated using vacuum liquid chromato-graphy for fractionation. Purification was performed using TLC preparative. Isolate then characterized using specific spray reagent, UV-Vis spectrophotometry and infrared spectrophotometry. Crude drug of Psidiumguajava contained flavonoid, tannin, quinone, saponin and steroid/ triterpenoid. Antioxidant activity of ethyl acetate extract is 65.63% with total phenol 4.25%, total flavonoid 0.53% and total tannin 1.16%. Antioxidant compound N was isolated from ethyl acetate extract.Antioxidant compound N was supposed to be aglycone flavone that has OH at C -4’, C-5 and C-7.Key words: Psidium guajava L. , antioxidant activity, ethyl acetate extract
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-01-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/660
10.14499/indonesianjpharm23iss1pp36-40
Indonesian Journal of Pharmacy; Vol 23 No 1, 2012; 36-40
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/660/526
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1284
2018-10-23T02:19:20Z
3:ART
Hypoglycemic Activity and Pancreas Protection of Combination Juice of Mengkudu (Morinda citrifolia Linn.) Juice and Temulawak (Curcuma xanthorrhiza Roxb.) Juice on Streptozotocin-Induced Diabetic Rats
Santoso, Bilal Subchan Agus; Academy of Pharmacy of Putra Indonesia Malang
Sudarsono, Sudarsono; Universitas Gadjah Mada
Nugroho, Agung Endro; Universitas Gadjah Mada
Murti, Yosi Bayu; Universitas Gadjah Mada
hypoglycemic, pancreas protection, combination juice, mengkudu, temulawak
Mengkudu fruit contains scopoletin and temulawak rhizome contains curcumin have been observed because they have strong antioxidant activity and they were used traditionally as antidiabetic. This research aimed to evaluate effect of antihyperglycemic and pancreas protection of juice combination of mengkudu fruit juice (MFJ) and temulawak rhizome juice (TRJ) on diabetes rats which were inducted with streptozotocin (STZ). Rats were grouped into 7 groups, each of group consisting 5 rats. Each of group was treated accordingly for 28 days except for a normal group of rats. Rats taken blood from the plexus retroorbitalis for examination of blood glucose levels every week. On the last day, rats blood was examined for malondialdehyde (MDA) levels. After that the rats were turned off for examination of pancreatic morphological conditions. The results showed that diabetic rats given MFJ-TRJ combination juice experienced a significant decrease in blood glucose levels, a significant decrease in MDA levels and improvement pancreas morphology when compared with the negative control group. The conclusion of this research was giving combination juice of MFJ-TRJ juice can decreased blood glucose level, decreased MDA level and can improved pancreas morphology condition.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Faculty of Pharmacy of Universitas Gadjah Mada
2018-04-17
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1284
10.14499/indonesianjpharm29iss1pp16
Indonesian Journal of Pharmacy; Vol 29 No 1, 2018; 16
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1284/848
Copyright (c) 2018 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/674
2020-01-27T02:51:49Z
3:ART
IDENTIFICATION OF SAFETY ALERT BY MONITORING ANALYTICAL PARAMETERS AND HIGH-RISK DRUGS
Vilaplana, Vicente Escudero-; Pharmacy Department, Gregorio Marañón
University General Hospital Doctor Esquerdo 46, 28007 Madrid, Spain
Antúnez, María Gómez-; Internal Medicine Department, Gregorio
Marañón University General Hospital, Madrid, Spain
García, Esther Durán-; Pharmacy Department, Gregorio Marañón
University General Hospital Doctor Esquerdo 46, 28007 Madrid, Spain
Míguez, Antonio Muiño-; Internal Medicine Department, Gregorio
Marañón University General Hospital, Madrid, Spain
Sáez, María Sanjurjo-; Pharmacy Department, Gregorio Marañón
University General Hospital Doctor Esquerdo 46, 28007 Madrid, Spain
Early detection of adverse drug reactions (ADR) increases patient safety. Our objective was to identify ADR by monitoring laboratory parameters and high-risk drugs. We carried out a two-month prospective observational study in a Internal Medicine Department, with daily recording of drugs prescribed and the following parameters: Na, K, Ca, serum creatinine, glomerular filtration rate (GFR), INR, glucose, haemoglobin, platelets, ALT, AST, bilirubin, GGT, alkaline phosphatase, TSH, T4, and blood digoxin. High-risk drugs were closely monitored. 52 patients included, of whom 46.2% experienced an ADR. We observed an association with drugs in 25.5%, as follows: reduction in GFR, 26.9% (associated with loop diuretics [41.7%], angiotensin-converting enzyme [ACE] inhibitors [33.3%], angiotensin II receptor blockers [ARB] [16.6%], andanti-diabetic drugs [8.3%]); hypokalemia, 22.3% (associated with loop diuretics [50.0%], potassium-free fluid [37.5%], and salbutamol [12.5%]); hyperkalemia, 14.4% (associated with ACE inhibitors [60.0%] and ARB [40.0%]); INR out of range, 10.8% (associated with drug interactions [66.7%]); hyperglycemia, 8.1% (associated with corticosteroids [66.7%] and anti-diabetic drugs [33.3%]); and other conditions, 18.8%. We conclued that patient safety could be improved by implementing warnings in electronic prescriptions in cases of a decrease in GFR or modification of potassium levels in patients who are prescribed loop diuretics, ACE inhibitors, or ARBs.Key words: Adverse drug reaction, clinical decision support, high-risk drug, safety
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2012-04-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/674
10.14499/indonesianjpharm23iss2pp130-134
Indonesian Journal of Pharmacy; Vol 23 No 2, 2012; 130-134
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/674/540
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1470
2022-10-26T01:05:53Z
3:ART
Quality of Life of Patient with Hypertension in Primary Health Care in Bandar Lampung
Khoirunnisa, Sudewi Mukaromah; Institut Teknologi Sumatera
Akhmad, Atika Dalili; Institut Teknologi Sumatera
Hypertension; Quality of Life; SF-36
The high prevalence of hypertension in Indonesia, encourages studies related to how the quality of life of patients with hypertension. The purpose of this study was to measure quality of life, identify and explain factors related to the quality of life of patients with hypertension.The study design was descriptive correlation using a cross sectional study approach. The study subjects were all outpatient hypertensive patients several health centers in Bandar Lampung. The instrument used in this study was the Indonesian SF-36. The data collected included the patient's demographic characteristics including gender, age, education, occupation, and marital status, and the fields related to the health history of the study subjects included the duration of hypertension, complications, and the number of antihypertensive drugs consumed. Data were analyzed using bivariate analysis to see the relationship between two variables with the level of significance used was 5% (α = 0.05) with the value of the confidence interval set was 95%. Multivariate analysis was conducted to study the relationship of several independent variables with one or several dependent variables.The results of univariate analysis showed that age, marital status, duration of illness, complications, and the number of drugs consumed had an effect on the quality of life of hypertensive patients (p <0.05). The results of multivariate analysis showed that the factors of age, marital status, and duration of hypertension are factors that influence the physical domain, while the factors of gender, marital status, duration of hypertension, complications, and the number of drugs are influential factors in the mental domain mental.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
The Ministries of Research, Technology and Higher Education of Indonesia
2020-01-02
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1470
10.14499/indonesianjpharm30iss4pp309
Indonesian Journal of Pharmacy; Vol 30 No 4, 2019; 309
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1470/927
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/downloadSuppFile/1470/200
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/825
2017-09-11T04:29:11Z
3:ART
GENOTYPE POLYMORPHISMS OF NAT2 AND CYP2E1 GENES ASSOCIATED WITH DRUG INDUCED LIVER INJURY (DILI) IN INDONESIAN TUBERCULOSIS PATIENTS
Perwitasari, Dyah Aryani; Faculty of Pharmacy, Kampus 3 Universitas Ahmad Dahlan, Jl Prof Dr Soepomo Janturan, yogyakarta
Currently, Indonesia is in the fifth rank of highest TB prevalence over the world. One of the TB problem is low patients’ adherence due to the oral antituberculosis induced hepatotoxicity. Polymorphisms of NAT2 and CYP2E1 genes had important role in the isoniazid (INH)-induced hepatotoxicity. The aim of this study was to evaluate the polymorphisms profile of NAT2 and CYP2E1 genes associated with hepatotoxicity induced by INH.We used cohort design in Public Health Centers and Lung Clinics of Yogyakarta and Lampung. The inclusion criteria were adult subjects (> 18 yo), newly diagnosed TB and treated by oral antituberculosis, normal function of renal and live and willingness to participate in this study. Subjects were excluded when having positive reaction of HbsAg test, history of HIV and abnormality of renal and liver function. The SNPs of NAT2 and CYP2E1 were designed using IPlex method of DNA sequenom.Among 57 TB patients, we found 14 patients with higher INH serum concentration and experienced increase of ALT-AST. Subjects with SNPs of rs 2070676, rs 1329149, rs 3813867, rs 6413432, rs 8192772, rs 2031920, rs 2515641, rs 8192775, rs 915908 of CYP2E1 experienced increase of ALT and AST. Subjects with SNPs of rs 1799930, rs1799931, rs1801279, rs1801280, rs1799929 , rs1208, rs1041983 of NAT2 are associated with the increase of ALT and AST.The polymorphisms of CYP2E1 and NAT2 may have a role in the mechanisms of INH induced DILI.Keywords: CYP2E1, NAT2, tuberculosis, isoniazid, Indonesia
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2017-08-25
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/825
10.14499/indonesianjpharm27iss1pp22
Indonesian Journal of Pharmacy; Vol 27 No 1, 2016; 22
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/825/816
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/414
2017-09-20T03:28:09Z
3:ART
Synthesis of Phenethyl-aza-ß-Lactam (1,2-Dimethyl4(R,S)-Phenethyl-[1,2]Diazetidin-3-One)
Ritmaleni, Ritmaleni; Faculty of Pharmacy, Gadjah Mada University, Yogyakarta Indonesia
Anggarwal, Varinder K; University of Bristol
Racemic spiro-epoxide, (7RS, 1RS, 3RS)-phenethyl-10-oxa-1,3-dithiaspiro[6,7]octane 1,3-dioxide 12, can be synthesised in four step reactions, sequences starting from the commercially available 1,3-dithiane 8. The synthesis of aza ß-lactam, 1,2-dimethyl-4(R,S)-phenethyl-[1,2]diazetidin-3one 13, was successfully carried out by opening the epoxide by 1,2-dimethyl hydrazine salt in moderate yield. Keywords : epoxide, aza-ß-lactam
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
2005-07-01
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/414
10.14499/indonesianjpharm0iss0pp162-166
Indonesian Journal of Pharmacy; Vol 16 No 3, 2005; 162-166
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/414/294
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
http://creativecommons.org/licenses/by-sa/4.0
oai:ojs.indonesianjpharm.farmasi.ugm.ac.id:article/1349
2019-10-10T01:29:33Z
3:ART
The Properties of Brown Marine Algae Sargassum turbinarioides and Sargassum ilicifolium Collected From Yogyakarta, Indonesia
Artemisia, Rahma; Stikes Madani Yogyakarta
Jl. Wonosari KM 10, Karanggayam, Sitimulyo, Piyungan, Bantul, DIY
Nugroho, Akhmad Kharis; Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
Setyowati, Erna Prawita; Department of Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
Martien, Ronny; Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
Sargassum turbinarioides, Sargassum ilicifolium, Fucoidan, Thin Layer Chromatography (TLC), Fourier transform infrared (FTIR) spectroscopy
Brown marine algae are the prominent source of marine natural products that have bioactive metabolites. Sargassum turbinarioides and Sargassum ilicifolium were dominated in Indonesia as brown marine algae that well known as a source of fucoidan. The samples were collected from Gunungkidul, Yogyakarta, Indonesia. In this study, we investigated and identified the yield of aqueous crude and purified extracts using different extraction temperatures (60°C, 70°C, 80°C, 90 °C). The highest yield of S. turbinarioides crude extract (7.36%) was obtained at temperatures 90 and 80 °C while the highest yield of S. ilicifolium was 3.49 % at 80 °C. The presence of sulfate polysaccharide in Sargassum turbinarioides is 3,78 % and Sargassum ilicifolium is 2,93 %. Each of the extract was screened using phytochemical detection, Thin Layer Chromatography (TLC) and Fourier transform infrared spectroscopy (FT-IR) analysis. The phytochemical detection indicated that Sargassum ilicifolium has bioactive metabolites such as carbohydrates, proteins and amino acids, terpenoid, phenolic compounds, and flavonoids. The FTIR spectrum of the S. turbinarioides and Sargassum ilicifolium extract refer to the presence of ester sulfate groups through showing peaks at 1300 to 1200 cm-1 and 980 to 950 cm-1. The result indicated that Sargassum turbinarioides and Sargassum ilicifolium contain of sulfate polysaccharide were prospect a biological activities to use for the development of marine nutraceutical drugs especially as antioxidant.
Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia
Ministry of Research, Technology, and Higher Education, Republic of Indonesia and also Beasiswa Unggulan Dosen Indonesia - Dalam Negeri (BUDI-DN) from Lembaga Pengelola Dana Pendidikan (LPDP) for sponsorship and financial support
2019-03-26
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
application/pdf
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1349
10.14499/indonesianjpharm30iss1pp43
Indonesian Journal of Pharmacy; Vol 30 No 1, 2019; 43
2338-9486
2338-9427
eng
https://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/1349/892
Copyright (c) 2019 Indonesian Journal of Pharmacy
http://creativecommons.org/licenses/by-sa/4.0
69343898c15586b8f9cc45368bd76a14