ANALYSIS OF ACTIVE COMPOUNDS IN BLOOD PLASMA OF MICE AFTER INHALATION OF CAJUPUT ESSENTIAL OIL (MELALEUCA LEUCADENDRON L.)

Muchtaridi Muchtaridi; Ami Tjiraresmi; Riska Febriyanti

doi:10.14499/indonesianjpharm26iss4pp219

ANALYSIS OF ACTIVE COMPOUNDS IN BLOOD PLASMA OF MICE AFTER INHALATION OF CAJUPUT ESSENTIAL OIL (MELALEUCA LEUCADENDRON L.)

Muchtaridi Muchtaridi, Ami Tjiraresmi, Riska Febriyanti

Abstract

The research is intended for the determination of volatile compounds of cajuput oil (Melaleuca leucadendron L.) in a group of mice (3 mice) after inhaling 1 mL cajuput oil for 1/2, 1 and 2 hour(s). The blood plasma of three mice was collected in heparin tube, and the volatile compounds were isolated and concentrated using C-18 Solid Phase Extraction (SPE) column with methanol and bidistilled water mixture (60:40) as the solvent. The analysis was carried out by GC/MS. The results showed that there were 52 compounds in the cajuput oil, especially 1,8-cineole,a-terpineol, b-pinene, a-pinene, b-myrcene, 4-terpineol, linalool, g-terpinene, and terpinolene. The major compound identified in blood plasma of mice was 1,8-cineole. Half an hour after 1 mL inhalation of cajuput oil per mouse, the concentration of 1,8-cineole identified was 41.5 mg/mL, while 1 and 2 hours after 1 mL inhalation of cajuput oil, the level of 1,8-cineole in blood plasma of 3 mice was 646.9 mg/mL and 138.0 mg/mL in blood plasma of each mouse. Other compounds identified were a-terpineol, 4-terpineol linalool, terpenyl acetate and hydrocarbon terpenes. Concentrations of target compounds in the blood plasma were relatively in low level (mg/mL). In conclusion, the volatile compounds of 4-terpineol, linalool, a-terpineol and 1,8-cineol that identified in blood plasma of mice after inhalation of the cajuput oils might correlate with depressive characteristics of essential oil given by inhalation.
Keywords: Cajuput, Essential Oils, Inhalation, SPE, GC-MS

Full Text:

PDF (219-227)

References

Adams, R. P., 1995, Identification of Essential Oil Components by Gas Chromatography/Mass Spectroscopy. Illinois: Allured Publishing Corporation.

Aoshima, H., S. J. Hossain, K. Hamamoto, T. Yokoyama, M. Yamada, and R. Shingai. 2001, Kinetic analyses of alcohol-induced potentiation of the response of GABA(A) receptors composed of alpha(1) and beta(1) subunits. Biochem 130 (5):703-709.

Buchbauer, G., 2004, On the biological properties of fragrance compounds and essential oils. Wien Med Wochenschr 154 (21-22):539-547.

Buchbauer, G., W. Jäger, L. Jirovetz, J. Ilmberger, and H. Dietrich. 1993. Therapeutic Properties of Essential Oils and Fragrances. In Bioactive Volatile Compounds from Plants: American Chemical Society, 159-165.

Buchbauer, G., and L. Jirovetz. 1994, Aromatherapy—use of fragrances and essential oils as medicaments. J Flav Frag 9 (5):217-222.

Buchbauer, G., L. Jirovetz, W. Jager, H. Dietrich, and C. Plank. 1991, Aromatherapy: evidence for sedative effects of the essential oil of lavender after inhalation. Z Naturforsch C 46 (11-12):1067-1072.

Buckle, J., 1999, Use of aromatherapy as a complementary treatment for chronic pain. Altern Ther Health Med 5 (5):42-51.

Diego, M. A., N. A. Jones, T. Field, M. Hernandez-Reif, S. Schanberg, C. Kuhn, V. McAdam, R. Galamaga, and M. Galamaga. 1998, Aromatherapy positively affects mood, EEG patterns of alertness and math computations. Int J Neurosci 96 (3-4):217-224.

Heyne, K., 1987, Tumbuhan Berguna Indonesia. Jakarta: Yayasan Sarana Wijaya.

Jirovetz, L., G. Buchbauer, W. Jager, A. Woidich, and A. Nikiforov. 1992, Analysis of fragrance compounds in blood samples of mice by gas chromatography, mass spectrometry, GC/FTIR and GC/AES after inhalation of sandalwood oil. Biomed Chromatogr 6 (3):133-134.

Jirovetz, L., W. Jager, G. Buchbauer, A. Nikiforov, and V. Raverdino. 1991, Investigations of animal blood samples after fragrance drug inhalation by gas chromatography/mass spectrometry with chemical ionization and selected ion monitoring. Biol Mass Spectrom 20 (12):801-803.

Kovar, K. A., B. Gropper, D. Friess, and H. P. Ammon. 1987, Blood levels of 1,8-cineole and locomotor activity of mice after inhalation and oral administration of rosemary oil. Planta Med 53 (4):315-318.

Lis-Balchin, M., 1999, Possible health and safety problems in the use of novel plant essential oils and extracts in aromatherapy. The journal of the Royal Society for the Promotion of Health 119 (4):240-243.

Martin, G. N., 1998, Human electroencephalographic (EEG) response to olfactory stimulation: two experiments using the aroma of food. Int J Psychophysiol 30 (3):287-302.

Muchtaridi, M., A. Diantini, and A. Subarnas. 2011, Analysis of Indonesian Spice Essential Oil Compounds That Inhibit Locomotor Activity in Mice. Pharmaceuticals 4 (4):590-602.

Muchtaridi, M., and I. Musfiroh. 2012, Off-Line SPE-GC/MS Analysis of Lead Compounds Aromatherapy in Blood Plasma of Mice of Essential Oils Materials from Indonesian Aromatic Plants. Asian J Chem 24 (11):5124-5128.

Muchtaridi, M., I. Musfiroh Ida, A. Subarnas, I. Rambia, H. Suganda, and M. E. Nasrudin. 2014, Chemical Composition and Locomotors Activity of Essential Oils from the Rhizome, Stem, and Leaf of Alpinia malaccencis (Burm F.) of Indonesian Spices. J Appl Pharmaceu Sci 04 (01):052-056.

Muchtaridi, M., A. Subarnas, A. Apriyantono, and R. Mustarichie. 2010, Identification of Compounds in the Essential Oil of Nutmeg Seeds (Myristica fragrans Houtt.) that Inhibit Locomotor Activity in Mice. International Journal of Molecular Sciences 11 (11):4771-4781.

Sangat, H., and Roematyo. 1996. Aromatherapy Plants: A Etnopharmacology Study. Paper read at Simposium Nasional I Tumbuhan Aromatik APINMAP, at Bogor.

Wiendah, H., A. Subarnas, and S. Supriyatna. 1999, Study of Locomotors Inhibitor Activity of Essential Oil of Cajuput (Melaleuca lecadendron), Department of Pharmacy, Universitas

DOI: http://dx.doi.org/10.14499/indonesianjpharm26iss4pp219