PROTECTIVE EFFECT ETHANOLIC EXTRACT OF Boesenbergia pandurata (ROXB.) Schlecht. AGAINST UVB-INDUCED DNA DAMAGES IN BALB/C MICE

Shanti Listyawati, Sismindari Sismindari, Sofia Mubarika, Yosi Bayu Murti

Abstract


Boesenbergia pandurata (Roxb.) Schlecht. contains bioactive compounds that have a number healthy effect including anti-oxidant and anti-carcinogenic activity. This research was carried out to examine the protective effect of B. pandurata extract against expression of cyclobutane pyrimidine dimers (CPDs) as marker of UVB-induced DNA damage in Balb/c mice. Dried powder of B. pandurata rhizomes was extracted by maceration method using 96% ethanol. The extract was quantified with pinostrobin as active marker using TLC scanner. Ethanolic extract of B. pandurata (EEBP) was given orally at 14 days before UV exposure with a variety doses, 0 (vehicle), 20, 40 and 60mg/kgBW/day and continuing until termination of the experiment. Following the UVB irradiation (1.4J/m2), mice were sacrificed at different time points (2, 24, 48, and 72h after UVB exposure). The back skin samples were collected to analyze CPDs expression by immunohistochemical method. The result showed that EEBP (contained 5% pinostrobin) dose was 40 and 60mg/kgBW/day had protective activity against UV-induced DNA damage as indicated by the decrease of CPDs expression.   

Key words:  Boesenbergia pandurata (Roxb.) Schlecht., UVB, DNA damage, CPDs.


Full Text:

PDF(PP.108-113)

References


Afaq F., Khan N., Syed DN., Mukhtar H., 2010. Oral Feeding of Pomegranate Fruit Extract Inhibits Early Biomarkers of UVB Radiation-Induced Carcino-genesis in SKH-1 Hairless Mouse Epidermis. J. Photochem. Photobiol. B: Biol. 86(6):1318–1326.

Cheenpracha S., Karalai C., Ponglimanont C., Subhadhirasakul S., Tewtrakul S., 2006. Anti-HIV-1 protease activity of compounds from Boesenbergia pandurata. J. Bioorg. Med. Chem. 14(6): 1710–1714.

Ching AYL., Wah TS., Sukari MA., Rahmani LM., Khalid K. 2007. Characterization of flavonoid derivatives from Boesenbergia rotunda (L.) Malay. J. Anal. Sci. 11(1): 154-159.

Isa NM., Abdul AB., Abdelwahab SI., Abdullah R., Sukari MA., et al., 2013. Boesenbergin A, a chalcone from Boesenbergia rotunda induces apoptosis via mitochondrial dysregulation and cytochrome c release in A549 cells in vitro: Involvement of HSP70 and Bcl2/Bax signalling pathways. J. Fung. Foods 5: 87-97.

Jing LJ., Mohamed M., Rahmat A., Bakar MFA. 2010. Phytochemicals, antioxidant properties and anticancer investigations of the different parts of several gingers species (Boesenbergia rotunda, Boesenbergia pulchella var. attenuata and Boesenbergia armeniaca). J. Med. Plant. Res. 4(1): 27-32.

Kamkaen N., Wilkinson JM., Cavanagh HMA., 2006. Cytotoxic Effect of Four Thai Edible Plants on Mammalian Cell Proliferation. J. Thai Pharma. Health Sci. 1(3):189-195.

Katiyar S., Elmets CA., Katiyar SK., 2007. Green tea and skin cancer: Photo-immunology, angiogenesis and DNA repair. J. Nutr. Biochem.18: 287–296.

Katiyar SK., 2007. Interleukin-12 and photocarcinogenesis. Toxicology and Applied Pharmacology.224: 220–227

Kim S., Oh HI., Hwang JK., 2012. Oral Administration of Fingerroot (Boesenbergia pandurata) Extract Reduces Ultraviolet B-induced Skin Aging in Hairless Mice. Food Sci. Biotech. 21(6): 1753-1760.

Kim MS., Pyun HB., Hwang JK, 2014. Panduratin A, an activator of PPAR-α/δ, suppresses the development of oxazolone-induced atopic dermatitis-like symptoms in hairless mice. Life Sciences. 100(1): 45–54

Kirana C., Jones GP., Record .R., McIntosh GH. 2007. Anticancer properties of panduratin A isolated from Boesenbergia pandurata (Zingiberaceae). J. Nat. Med. 61:131–137

Listyawati S., Sismindari Mubarika S., Murti, YB., 2012. Aktivitas Kemoprevensi Ekstrak Temu Kunci (Boesenbergia pandurata) pada Karsinogenesis Kulit Mencit Balb/c Terinduksi Ultra Violet, Seminar Nasional IX, Biologi, Sains, Lingkungan, dan Pembelajarannya dalam Upaya Peningkatan Daya Saing Bangsa, Surakarta: Program Studi pendidikan Biologi, FKIP, Universitas Sebelas Maret Surakarta, hal. 612-616.

Nihlati I., Rohman A., Hertiani T., 2008. Daya Antioksidan ekstrak etanol rimpang temu kunci (Boesenbergia pandurata (Roxb.) Schlecth.) dengan metode radikal DPPH (1,1=difenil-2-pikrilhidrasil. Majalah Obat Tradisional. 13:45.

Marrot L., Meunier JR., 2008. Skin DNA photodamage and its biological Consequences. J. Am. Acad. Dermatol. 58: S139-148.

Matsumura Y., Ananthaswamy HN., 2004. Toxic effects of ultraviolet radiation on the skin. Tox. Appl. Pharmacol. 195(3): 298–308.

Meeran SM., Akhtar S., Katiyar SK. 2009. Inhibition of UVB-Induced Skin Tumor Development by Drinking Green Tea Polyphenols is Mediated Through DNA Repair and Subsequent Inhibition of Inflamation. J. Invest. Dermatol. 129 (5): 1258-1270.

Nichols JA., Katiyar SK., 2010. Skin photoprotection by natural polyphenols: Anti-inflammatory, anti-oxidant and DNA repair mechanisms. Arch. Dermatol. Res. 302(2): 71.

Pérez-sánchez A., Barrajón-catalán E., Caturla N., Castillo J., Benavente-garcía O., 2014. Protective effects of citrus and rosemary extracts on UV-induced damage in skin cell model and human volunteers. J. Photochem. Photobiol. B: Biol. 136: 12–18.

Roche M., Seagrove S., Mehta A., Divekar P., Campbell S., Curnow A. 2010. Using natural dietary sources of antioxidants to protect against ultraviolet and visible radiation-induced DNA damage: an investigation of human green tea ingestion. J. Photochem. Photobiol. B: Biol. 101(2): 169–173.

Saraithong P., Saenphet S., Saenphet K., 2010. Safety Evaluation of Ethanol Extracts from Bosenbergia rotunda (L.) Mansf. in Male Rats. Trends Research in Science and Technology. 2 (1): 19-22

Shindo K., Kato M., Kinoshita A., Kobayashi A., Koike Y., 2006. Analysis of antioxidant activities contained in the Boesenbergia pandurata Schult. Rhizome. Biosci. Biotech. Biochem.70(9): 2281–2284.

Tanjung M., Tjahjandarie TS., Sentosa MH., 2013. Antioxidant and cytotoxic agent from the rhizomes of Kaempferia pandurata. Asian Pac. J. Tropic. Dis. 3(5): 401-404.

Vaid M., Katiyar SK., 2010. Molecular mechanisms of inhibition of photocarcinogenesis by silymarin, a phytochemical from milk thistle (Silybum marianum L. Gaertn). Int. J. Oncol. 36(5): 1053–1060.

Widyarini S., 2006. Protective effect of the isoflavone equol against DNA damage induced by ultraviolet radiation to hairless mouse skin. J. Vet. Sci., 7(3): 217-223

Wölfle U., Philipp R., Esser PR., Simon-Haarhaus B., Martin SF. Lademann J., Schempp CM., 2011. UVB-induced DNA damage, generation of reactive oxygen species, and inflammation are effectively attenuated by the flavonoid luteolin in vitro and in vivo. Free Radic. Biol. Med. 50: 1081–1093.

You Y., Szabo PE., 2000. Cyclobutane pyrimidine dimers form preferentially at the major p53 mutational hotspot in UVB-induced mouse skin tumors. Carcinogenesis. 21(11): 2113–2117

Zattra E., Coleman C., Arad S., Helms E., Levine D., et al., 2009. Polypodium leucotomos Extract Decreases UV-Induced Cox-2 Expression and Inflammation, Enhances DNA Repair, and Decreases Mutagenesis in Hairless Mice. AJP. 175(5): 1952-1960.




DOI: http://dx.doi.org/10.14499/indonesianjpharm26iss2pp108

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Indonesian J Pharm indexed by:

                                                

web
analytics View My Stats