CHEMICAL CONSTITUENT DETECTION AND OXYTOXIC EFFECT OF INSOLUBLE ETHANOLIC FRACTION OF INFUSION OF Kaempferia angustifolia Roscue LEAVES ON ISOLATED UTERUS OF GUINEA PIGS

Pramono S., Sumastuti R.

Abstract


To establish the scientific base on the use of Kaempferia angustifolia leaves as Indonesian post partus traditional drug, an experiment was done on isolated uterus guinea pigs. The infusion of Kaempferia angustifolia leaves was evaporated and the residu was then extracted with ethanol. The ethanolic fraction and the insoluble fraction were tested on isolated uterus guinea pig after addition of 1 ml of 10 IU/ml oxytocin. The active fraction was then dissolved in distlled water in order temterature. The chemical constituents of the active fraction were detected by thin layer chromatographic method. The results showed that the insoluble ethanolic fraction of the infusion increased significantly the contraction of uterus after the addition of oxytocin (p<00.5). Further more, the water soluble fraction and the residue of the active fraction had also similar activities.

Keywords : Kaempferia angustifolia, oxytoxic effect, flavonoids, tanin, pectin


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References


Backer, C.A. dan Van den Brink, R.C.B., 1965, Flora of Java, NVP. Noordhoff, Groningen, The Netherlands. 122-123

Bruneton, J., 1992, Pharmacognosie, Phytochimie Plantes Medicinales, deuxieme edition, Lavoisier, Paris, p. 106-108, 266-280.

Heyne, K., 1950, De Nuttige Planten van Indonesie, terjemahan oleh Badan Litbang Kehutanan, 1987, Jakarta, p.529-530.

Jork, H., Funk, W., Fischer, W., Wimmer, H., 1990, Thin Layer Chromatography, Reagents and Detection Methods, Volume 1, VCH Verlagsgesellschaft, Weinheim, p. 44, 288, 299.

Turner, R.A., 1965, Sreening Methods in Pharmacology, Academic Opress, New York, p. 98-99.

Wagner, H., Bladt, S., Zgainski, E.M., 1984, Plant Drug Analysis, Springer Verlag, Berlin, p. 291-295.




DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp114-118

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