LABELING OF MIBI (METOXY ISOBUTYL ISONITRYL) WITH TECHNETIUM-99m AS A PERFUSION MYOCARDIAC IMAGING RADIOPHARMACEUTICAL
Coronary artery disease is one the major causes of death in Indonesia. For early diagnosis of the disease, it is deemed necessary to visualize the myocardium blood flow perfusion. 99mTc-MIBI labeled compound is one of the agent that could be used for such purpose. The labeling of MIBI with technetium-99m under some variations of several parameters has been carried out. The labeling efficiency was determined by counting the radiochemical purity using ITLC-SG 60 thin layer chromatography using two kinds of solvents, i.e. methanol and 0.9% NaCl solutions. The optimum labeling condition was achieved at the pH = 5.5 – 6.0, 1 mg of [Cu(MIBI)4BF4, 0.060 mg SnCl2.2H2O and 10 minutes incubation time in a boiling water bath, gave ± 99% of labeling efficiency. Under the formulation stated above, MIBI dry kit produces could give the radiochemical purity of more than 95%.
Key words : metoxy isobutyl isonitril (MIBI), technetium-99m, labeling, myocardiac perfusion imaging.
English R.J., Kozlowski J., Tumeh S.S., Holman B.L., 1987, 99mTc-Myocardial Perfusion Agent : an Introduction J. Nucl. Med. Technol., 15, 138.
Holman B.L., Jones A.G., Lister-James J., 1984, A new 99mTc-labelled Myocardial Imading Agent, Hexakis(tbutylisonitrile)-technetium(I) (99mTc-TBI). Initial Experience in the Human, J. Nucl. Med., 25, 1350 –1355.
Holman B.L., Sporn V., Jones A.G., 1987, Myocardial Imaging with 99mTc-CPI : Initial Experience in the Human, J.Nucl.Med., 28, 13 – 18.
Jozsef K., 1991, Personal Communication, Institute of Isotopes of the Hungarian, Academy of Sciences, Budapest, Hongary.
Khamis S.B., Ab-Wahid M., 1998, Development of MIBI Kit for Heart Imaging, in : Modern Trend in Radiopharmaceuticals for Diagnosis and Therapy, Proceeding of Symposium, IAEA-TECDOC-1029, Lisbon, 249 – 255.
Thongyoi C., 1994, Preparation and Quality Control of Technetium-99m Metoxyisobutyl isonitril (MIBI) for Myocardial Imaging, IAEA Research Contract No. 6708/RB.
Van Aswegen A., Van Wyk A.J., Herbst C.P., Otto A.C., Knoesen O., Lotter M.G., Mirinaar P.C., Kleynhans P.H.T., Fourie P.J., 1989, Pictures in the Heart. Nuclear Active, Int. J. of the AEC, 41, 42 – 45.
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