COBALT INDUCTION ON BIOSYNTHESIS OF CYANOCOBALAMIN by Streptomyces olivaceus IFO 3409.
The structure of cyanocobalamin containing an essensial cobalt is as part the compound. The aim of this study was to investigate the influence of cobalt on biosynthesis of cyanocobalamin done by Streptomyces olivaceus IFO 3409. This experiment was carried out by cultivating S. olivaceus in fermentation medium contained glucose 1,5%, pepton 1%, yeast extract 0,25%, trace mineral and each of medium was added cobalt 5, 10, 20 ppm. Cyanocobalamin synthesized by the cells was harvested on the last exponential phase. The amount of cyanocobalamin was analyzed by spectrophotometer. The results show that no cyanocobalamin was produced by S. olivaceus culture without kobalt or treated with 5 ppm cobalt on medium. However cyanocobalamin was produced if the culture medium was treated with 10 and 20 ppm cobalt respectively. It was concluded that cobalt induced on cyanocobalamin biosyntesis, therefore optimation of adding kobalt might be examined on getting maximal cyanocobalamin produced by Streptomyces olivaceus IFO 3409.
Key words: cyanocobalamin, cobalt, Streptomyces olivaceus IFO 3409.
Bykhovsky, V.Y., Demain, A.L., & Zaitseva, N.l., 1997, The Crucial Contribution of Starved Resting Cell to Elucidation of the Pathway of Vit B -12 Biosynthesis, Crit. Rev. Biotech, 17:17-28
Crueger, W & Crueger, A., 1989, Biotechnology : A Textbook of Industrial Microbiology, Science Tech., Inc. 187-189
Fazzio, F.G & Roth, J.R., 1996, Evidence that Cys G Protein Catalyses the First Reaction Specific to B-12 Synthesis in Salmonella Typhimurium, Insertion of Cobalt, J. Bacteriol, 178, 6952-6954
Kasanah, N., 2000, Isolasi Sianokobalamin dari Kultur Fermentasi Streptomyces olivaceus IFO 3409., Media Gama 2 (1)
Madigan, M.T., Martinko, J.M., Parker, J., 1997, Brock Biology of Microorganism, Prentice Hall, 447-448
- There are currently no refbacks.
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Indonesian J Pharm indexed by:View My Stats