ESTIMATION OF PROTEIN CONCENTRATION IN FOOD BY TOTAL NITROGEN AND AMINO ACID ANALYSES

Sumarno ., Sri Noegrohati, Narsito ., Iip Izul Falah

Abstract


Since protein is the principal constituent of biological organs, a continuous supply is needed in food for growth and repair. Protein quality in food is determined by its assortment of amino acids, whether it is essentially needed or not. The aim of this study is to develop an accurate and precise simple method of protein estimation for food and feed. The validity of simple protein estimation by multiplying the N-total concentration with a constant factor: of 6.25, should be clarified. Protein could be estimated more specifically by summing up the determined amino acids. In this study, the amino acids of some substantially high protein food were analysed by HPLC-ninhydrin, HPLC-o-phthalaldehyde, and HPLC-dansyl techniques, by gas chromatograph after derivation with TFA-butanol, and by formol titration. Method based on HPLC-ninhydrin techniques shows significant correlation (r=0-9992) with its amino acids content and N-total content. The protein as amino acids - N-total conversion factor obtained was 6.95 for N-total content less than 15.5%, and 8.59 for N-total content higher than 15.5%.

Key words: food, protein and analytical methods


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References


Bodanszky M., 1993,Chemistry of Peptide, Springer-Verlag, Berlin 47-52

Fennema, O.W., 1976, Principles of Food Science, Food Chemistry, Part I, Marcel Dekker, Inc, New York.

Grob, R.L., 1977, Modern Practice of Gas Chromatography, John Willey & Sons, New York.

Latupeiressa, S.B., Hadi, H., dan Susilowati., 2000, Perilaku Ibu Hamil Kurang Energi Kronik dalam Program Pemberian Makanan Tambahan Pemulihan di Kecamatan Wates dan Temon N Kabopaten Kulon Progo. Sins Kesehatan 13 1-14.

Lehninger, A.L., 1975, Biochemistry, The Moleculear Basis of Cell Structure and Function, 2nd. Ed., Publisher Inc.

Linder M.C., 1992, Biokimia, Nutrisi dan Metabolisme (diterjemahkan oleh Aminuddin dan Amwila A,Y). Universitas Indonesia Press. Jakarta 89-115.

Lottspeich, F., A. Henschen, K.P. Hupe (Editors), 1981, High Performance Liquid Chromatography in Protein and Peptide Chemistry: Proceedings of International Symposium, January 1981, at Max-Planck-Institute for Biochemistry, Munich, Walter de Gruyter, Berlin.

Lydia,K, 1997, Kadar Albumin Serum dan Faal Ginjal Anak, Bul.Penel, Kes 25, 20-26

Maynard, L.A., J.K. Loosli, H.F. Hintz, R.G. Warner, 1984, Animal Nutrition, 7th edition, Tata McGraw – Hill Pub. Co. Ltd, New Delhi.

Sihadi, 1998/1999, Beberapa Faktor yang Berhubungan dengan Gizi dari Gizi Buruk Menjadi Gizi Kurang di Klinik Gizi Bogor (KGB), 1982-1997, Bul Pen. Kes.26 47-62.

Smith, R.M., 1988, Gas and Liquid Chromatography in Analytical Chemistry, John Willey & Sons, Chichester.

Sya’bani, M, 1998, Arti Klinis Pemeriksaan Albumin Serum Sebagai Pertanda Progres Malnutrusi dengan Metode Brom Cresol Green (BCG), dan Elektroforesis pada Penderita Hemodialisis Rutin, B.I.Ked. 30 , 181-187




DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp34-43

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