PRODUCTION OF QUININE ALKALOID BY SOME ENDOPHYTIC MICROBES WITH ADDITION OF INDUCER SUBSTANCES (Studies on Endophytic Microbes of Cinchona sp. plants (2))
Endophytic microbes have been known to be potential as the sources of active compound for medicines. Optimization of medium, including of inducer addition, is necessary to obtain optimum products. This study, therefore, was aimed to investigate the effect of Cinchona succirubra bark powder addition in the production of quinine compound by endophytic microbe growing in two different liquid media. Endophytic microbe isolated from Cinchona sp. were shaker incubated in Potato Dextrose Broth (PDB) and Phoma media with the addition of bark powder of C. succirubra for 3 days. Quinine production was analyzed with High Performance Liquid Chromatography (HPLC). The result showed an increasing of quinine production for each microbes. Highest increasing of quinine production (2.2 folds) was yielded by mold culture (J3P1) grown in PDB medium.
Keywords: inducer, quinine, endophytic microbe, Cinchona succirubra
Gumbira, S.E., 1987. Bioindustri Penerapan Teknologi Fermentasi, edisi I, PT. Mediayatama Sarana Perkasa, Jakarta, 1987, 27
Hostettmann, K., Hamburger M., Hostettmann M., dan Marston A.,. 1991. New Developments in Separation of Natural Product., Di dalam Modern Phytochemical Methods, (edit. N.H. Fischer et al.) Plenum Press, 1 – 31
Musalam, Y., Sukasmono, Suhartika T., dan Supria. 1980. Alkaloid Kuinina di dalam Tanaman Cinchona sp., WARTA BPTK, 6, 85-93
Pelczar, M. dan Chan. C.S. 1988. Dasar-dasar Mikrobiologi, UI Press Jakarta, hal.70.
Petrini, O., Sieber T.N., Toti L., dan Viret O., 1992. Ecology, Metabolite Production and Utilization in Endophytic Fungi, Wiley Liss Inc., 78
Simanjuntak, P., Titi Parwati, Bustanussalam, Titik K. Prana, Ohashi K. dan Shibuya H., 2002. Biochemical Character of Endophyte Microbes Isolated from Cinchona plants, Proceeding Seminar on JSPS-NCRT/DOST/LIPI/VCC Large Scale Cooperative Research in the Field of Biotechnology, in Bangkok, Thailand Nov. 7 – 12, 2001 (In Press)
Song, Y. 1998. Isolation and Cultivation of Endophytic Fungi, Asian Network on Microbial Reseacher, Gadjah Mada university (GMU), Yogyakarta, 1998, 255 – 258
Stierle, A., Strobel G., dan Stierle D., 1993. Taxol and Taxane Production by Taxomyces andreana, an Endophytic Fungus of Pacific Yew, Sciences, 260, 214-216
Strobel, G.A., Stierle A., dan Hess W.M., 1993. Taxol Formation in Yew-Taxus, Plant Sciences, 92, 1 – 12
Tanaka, Y.,1992, Fermentation Processes in Screening for new Bioactive Substances, in The Search for Bioactive Compounds from Microorganism, (editor Satoshi), Springer-Verlga Press., 303-325
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