SELAGINELLA ACTIVE FRACTIONS INDUCE APOPTOSIS ON T47D BREAST CANCER CELL

Sri Handayani, Chandra Risdian, Edy Meiyanto, Zalinar Udin, Rina Andriyani, Marissa Angelina

Abstract


Apoptosis  is  an  important  target  on  anticancer  mechanism. The  purpose  of  this  research  is  to  investigate  apoptosis  induction of Selaginella plana Hieron active fractions on T47D cells. Absolute ethanol  was  used  to  extract Selaginella  plana powders.  Ethanolic extract  was  dilluted  by  methanol:water  (4:1)  and  then fractionated  by  hexane  (S_Hex),  methylene  chloride  (S_MTC), ethyl acetate (S_EA), and buthanol (S_BuOH). The proliferation of T47D  cell  line  was  detected  by  SRB  (Sulforhodamine  B) assay which  was  measured  at  a  wavelength  of  515nm.  Flowcytometry analysis  to  determine  apoptosis  was  examined  by  Propidium Iodide  (PI)  and  Annexin  V  assay  using  T47D  breast  cancer  cell line.  The  result  showed  that  the  IC50 value  of  S_Hex,  S_MTC, S_EA,  and  S_BuOH  on  T47D  cells  were  107  µg/mL,  4  µg/mL,  6 µg/mL,  and  17  µg/mL  respectively.  The  active  fractions  (S_MTC and  S_EA)  at  its  IC50 concentration  significantly  (P<0.05) increased  the  total  number  of  early  apoptotic  cells  in  the  T47D cells  3.39%  and  4.1%  respectively  compared  to  that  of  control (1.95%).  Based  on  the  result,  methylene  chloride  and  ethyl acetate  fraction  of Selaginella  plana induced  apoptosis  on  T47D cell.

Keywords: apoptosis, breast cancer, Selaginella


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References


Gulati, N., Laudet, B., Zohrabian, V.M., Murali, R., and Jhanwar-Uniyal, M., 2006, The antiproliferative effect of Quercetin in cancer cells is mediated via inhibition of the PI3K-Akt/PKB pathway, Anticancer Res., 26(2A):1177-1181.

Hanahan, D.,and Weinberg, R.A.,2000, The Hallmarks of Cancer,Cell, 100: 57-70.

Handayani, S., and Udin, Z., 2011, Molecular Docking of Robustaflavone Derivatives On PI3K Protein Target and The Correlation With Its Cytotoxic Activity On Cancer Cells, IJCC, Manuscrips to be published.

Harborne, J.B., 1987, Metode Fitokimia (Penuntun Cara Modern Menganalisis Tumbuhan), Terjemahan K. Padmawinata dan I. Soedira, Penerbit ITB, Bandung.

Kaur, P., Shukla, S., and Gupta, S., 2008, Plant flavonoid apigenin inactivates Akt to trigger apoptosis in human prostate cancer: an in vitro and in vivo study, Carcinogenesis, 29(11):2210-2217.

Lee, N.Y., Min, H.Y., Lee, J., Nam, J.W., Lee, Y.J., Han, A.R., Wiryawan, A., Suprapto, W., Lee, S.K., and Seo, E.K., 2008, Identification of a New Cytotoxic Biflavanone from Selaginella doederleinii,

Chem. Pharm. Bull., 56(9):1360—1361.

Liu,W., Cheung, F.W.K., Liu, B.P.L., Li, C., Ye, W., and Che, C., 2008, Involvement of p21 and FasL in induction of cell cycle arrest and apoptosis by neochamaejasmin A in human prostate LNCaP cancer cells, J Nat Prod),71(5): 842-846.

Ma, S., But, P.P.,Ooi, V.E., He., Y., Lee, S.H., Lee, S., and Lin, R., 2001, Antiviral Amentoflavone from Selaginella sinensis, Biol. Pharm. Bull., 24(3): 311-312.

Markowitz, S.D., 2007, Aspirin and Colon Cancer - Targeting Prevention?, N Engl J Med, 356(21):2195-2198.

Pope, R.M., 2002, Apoptosis as a therapeutic tool in rheumatoid arthritis, Nature Reviews Immunology, 2:527-535.

Reuter, C.W.M., Morgan, M.A., and Bergmann, L., 2000, Targeting the Ras signaling pathway: a rational, mechanism-based treatment for hematologic malignancies?, Blood. 96:1655-1669.

Shafi, G., Munshi, A., Hasan, T., Alshatwi, A.A., Jyothy, A., and Lei, D.K.Y., 2009, Induction of apoptosis in HeLa cells by chloroform fraction of seed extracts of Nigella sativa, Cancer Cell International, 9:29.

Silva,G.L., Chai, H., Gupta, M.P., Farnsworth, N.R., Cordell, G.A., Pezzuto, J.M., Beecher, C.W.W., and Kinghorn, A.D., 1995,Cytotoxicbiflavonoids from Selaginellawilldenowii, Phytochemistry, 40(1): 129-134.

Simstein, R., Burow, M., Parker, A., Weldon, C., and Beckman, B., 2003, Apoptosis, Chemoresistance, and Breast Cancer: Insights from The MCF-7 Cell Model System, Exp Biol Med,228:995–1003.

Su, Z., Madireddi, M.T., Lin, J.J., Young, C.S.H., Kitada S., Reed, J.C., Goldstein, N.I., and Fisher, P.B., 1998, The cancer growth suppressor gene mda-7 selectively induces apoptosis in human breast cancer cells and inhibits tumor growth in nude mice, Proc. Natl. Acad. Sci., 95: 14400–14405.

Sun, S.Y., Hail, N., and Lotan, R., 2004, Apoptosis as a Novel Target for Cancer Chemoprevention, J Natl Cancer Inst, 96:662–72.

Tan, W.J., Xu, J.C., Li, L., Chen, K.L., 2009, Bioactive compounds of inhibiting xanthineoxidase from Selaginella labordei, Nat Prod Res., 23(4): 393-398.

Xavier, C.P.R., Lima, C.F., Preto, A., Seruca, R., Ferreira,M.F., and Wilson, C.P., 2009, Luteolin, quercetin and ursolic acid are potent inhibitors of proliferation and inducers of apoptosis in both KRAS and BRAF mutated human colorectal cancer cells, Cancer Letters, 281(2):162-170.

Yoon, S., Shin, S., Lee, H., Chun, H., and Chung, A., 2006, Isoginkgetin inhibits tumor cell invasion by regulating phosphatidylinositol 3-kinase/Akt–dependent matrix metalloproteinase-9 expression, Mol Cancer Ther, 5(11):2666–2675.

Zhou, W., Horstick, E.J., Hirata, H., and Kuwada, J.Y. (2008) Identification and expression of voltage-gated calcium channel beta subunits in Zebrafish. Dev. Dyn. 237(12): 3842-3852




DOI: http://dx.doi.org/10.14499/indonesianjpharm0iss0pp48-53

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