SELAGINELLA ACTIVE FRACTIONS INDUCE APOPTOSIS ON T47D BREAST CANCER CELL
Apoptosis is an important target on anticancer mechanism. The purpose of this research is to investigate apoptosis induction of Selaginella plana Hieron active fractions on T47D cells. Absolute ethanol was used to extract Selaginella plana powders. Ethanolic extract was dilluted by methanol:water (4:1) and then fractionated by hexane (S_Hex), methylene chloride (S_MTC), ethyl acetate (S_EA), and buthanol (S_BuOH). The proliferation of T47D cell line was detected by SRB (Sulforhodamine B) assay which was measured at a wavelength of 515nm. Flowcytometry analysis to determine apoptosis was examined by Propidium Iodide (PI) and Annexin V assay using T47D breast cancer cell line. The result showed that the IC50 value of S_Hex, S_MTC, S_EA, and S_BuOH on T47D cells were 107 µg/mL, 4 µg/mL, 6 µg/mL, and 17 µg/mL respectively. The active fractions (S_MTC and S_EA) at its IC50 concentration significantly (P<0.05) increased the total number of early apoptotic cells in the T47D cells 3.39% and 4.1% respectively compared to that of control (1.95%). Based on the result, methylene chloride and ethyl acetate fraction of Selaginella plana induced apoptosis on T47D cell.
Keywords: apoptosis, breast cancer, Selaginella
Gulati, N., Laudet, B., Zohrabian, V.M., Murali, R., and Jhanwar-Uniyal, M., 2006, The antiproliferative effect of Quercetin in cancer cells is mediated via inhibition of the PI3K-Akt/PKB pathway, Anticancer Res., 26(2A):1177-1181.
Hanahan, D.,and Weinberg, R.A.,2000, The Hallmarks of Cancer,Cell, 100: 57-70.
Handayani, S., and Udin, Z., 2011, Molecular Docking of Robustaflavone Derivatives On PI3K Protein Target and The Correlation With Its Cytotoxic Activity On Cancer Cells, IJCC, Manuscrips to be published.
Harborne, J.B., 1987, Metode Fitokimia (Penuntun Cara Modern Menganalisis Tumbuhan), Terjemahan K. Padmawinata dan I. Soedira, Penerbit ITB, Bandung.
Kaur, P., Shukla, S., and Gupta, S., 2008, Plant flavonoid apigenin inactivates Akt to trigger apoptosis in human prostate cancer: an in vitro and in vivo study, Carcinogenesis, 29(11):2210-2217.
Lee, N.Y., Min, H.Y., Lee, J., Nam, J.W., Lee, Y.J., Han, A.R., Wiryawan, A., Suprapto, W., Lee, S.K., and Seo, E.K., 2008, Identification of a New Cytotoxic Biflavanone from Selaginella doederleinii,
Chem. Pharm. Bull., 56(9):1360—1361.
Liu,W., Cheung, F.W.K., Liu, B.P.L., Li, C., Ye, W., and Che, C., 2008, Involvement of p21 and FasL in induction of cell cycle arrest and apoptosis by neochamaejasmin A in human prostate LNCaP cancer cells, J Nat Prod),71(5): 842-846.
Ma, S., But, P.P.,Ooi, V.E., He., Y., Lee, S.H., Lee, S., and Lin, R., 2001, Antiviral Amentoflavone from Selaginella sinensis, Biol. Pharm. Bull., 24(3): 311-312.
Markowitz, S.D., 2007, Aspirin and Colon Cancer - Targeting Prevention?, N Engl J Med, 356(21):2195-2198.
Pope, R.M., 2002, Apoptosis as a therapeutic tool in rheumatoid arthritis, Nature Reviews Immunology, 2:527-535.
Reuter, C.W.M., Morgan, M.A., and Bergmann, L., 2000, Targeting the Ras signaling pathway: a rational, mechanism-based treatment for hematologic malignancies?, Blood. 96:1655-1669.
Shafi, G., Munshi, A., Hasan, T., Alshatwi, A.A., Jyothy, A., and Lei, D.K.Y., 2009, Induction of apoptosis in HeLa cells by chloroform fraction of seed extracts of Nigella sativa, Cancer Cell International, 9:29.
Silva,G.L., Chai, H., Gupta, M.P., Farnsworth, N.R., Cordell, G.A., Pezzuto, J.M., Beecher, C.W.W., and Kinghorn, A.D., 1995,Cytotoxicbiflavonoids from Selaginellawilldenowii, Phytochemistry, 40(1): 129-134.
Simstein, R., Burow, M., Parker, A., Weldon, C., and Beckman, B., 2003, Apoptosis, Chemoresistance, and Breast Cancer: Insights from The MCF-7 Cell Model System, Exp Biol Med,228:995–1003.
Su, Z., Madireddi, M.T., Lin, J.J., Young, C.S.H., Kitada S., Reed, J.C., Goldstein, N.I., and Fisher, P.B., 1998, The cancer growth suppressor gene mda-7 selectively induces apoptosis in human breast cancer cells and inhibits tumor growth in nude mice, Proc. Natl. Acad. Sci., 95: 14400–14405.
Sun, S.Y., Hail, N., and Lotan, R., 2004, Apoptosis as a Novel Target for Cancer Chemoprevention, J Natl Cancer Inst, 96:662–72.
Tan, W.J., Xu, J.C., Li, L., Chen, K.L., 2009, Bioactive compounds of inhibiting xanthineoxidase from Selaginella labordei, Nat Prod Res., 23(4): 393-398.
Xavier, C.P.R., Lima, C.F., Preto, A., Seruca, R., Ferreira,M.F., and Wilson, C.P., 2009, Luteolin, quercetin and ursolic acid are potent inhibitors of proliferation and inducers of apoptosis in both KRAS and BRAF mutated human colorectal cancer cells, Cancer Letters, 281(2):162-170.
Yoon, S., Shin, S., Lee, H., Chun, H., and Chung, A., 2006, Isoginkgetin inhibits tumor cell invasion by regulating phosphatidylinositol 3-kinase/Akt–dependent matrix metalloproteinase-9 expression, Mol Cancer Ther, 5(11):2666–2675.
Zhou, W., Horstick, E.J., Hirata, H., and Kuwada, J.Y. (2008) Identification and expression of voltage-gated calcium channel beta subunits in Zebrafish. Dev. Dyn. 237(12): 3842-3852
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