ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF RED Psidium guajavaL. LEAVES GROWN IN MANOKO, LEMBANG - INDONESIA
Psidium guajava L. (Myrtaceae) is a well known plant in Malaysia and Indonesia. Its leaves extract was found to possess antidiarrhea, antimicrobial, hepatoprotective and antioxidant activities. Objective of this research is to isolate an antioxidant subtance from Red Psidium guajava L. leaves. The crude leaves was extracted using Soxhlet apparatus by gradual polarity of three different solvents, n-hexane, ethyl acetate and methanol. Antioxidant activity of each extract was tested by using DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method. Total phenol, total flavonoid and total tannin content of the extracts were also measured. Ethyl acetate extract was fractionated using vacuum liquid chromato-graphy for fractionation. Purification was performed using TLC preparative. Isolate then characterized using specific spray reagent, UV-Vis spectrophotometry and infrared spectrophotometry. Crude drug of Psidiumguajava contained flavonoid, tannin, quinone, saponin and steroid/ triterpenoid. Antioxidant activity of ethyl acetate extract is 65.63% with total phenol 4.25%, total flavonoid 0.53% and total tannin 1.16%. Antioxidant compound N was isolated from ethyl acetate extract.
Antioxidant compound N was supposed to be aglycone flavone that has OH at C -4’, C-5 and C-7.
Key words: Psidium guajava L. , antioxidant activity, ethyl acetate extract
Bedawey, A.A., 2010, Characteristics of Antioxidant Isolated from Some Plants Sources, Department of Food Science and Technology, Faculty of Agriculture, Menufiya University, Shibin El-Kom, Egypt, 1-11.
Ikegami, F, 2001, Antioxidant Active Principle Isolated from Psidium guajava Grown in Thailand, dissertation, Faculty of Pharmacy, Chiang Mai University, Thailand, 179-190.
Ditjen POM Depkes RI, 1989, Materi Medika Indonesia, Jilid V. Depkes RI. Jakarta
Mabry T.J., Markham, K.R. and Thomas, M.B. 1970, The Systematic Identification of Flavonoids. Springer Verlag, Berlin.
Metwally, A.M., A.A. Omar, N. M. Ghazy and F. M. Harraz, S. M. El Sohafy, 2011, Pharmacognosy Journal, Monograph of Psidium guajava L. leaves.
OrdonEz, A.A.L., J.D. Gomez, M.A. Vattuone, and M.I. Isla, 2006, Antioxidant activities of Sechium edule (Jacq.) Swart extracts, Food Chem., 97: 452-458.
Pourmorad, F., S.J. Hossinimehr, N. And Shahabimajd, 2006, Antioxidant Activity, Phenol and Flavonoid Contents of Some Selected Iranian Medicinal Plants, Afr. J. Biotechnol., 5(11), 1142-1145
- There are currently no refbacks.
Copyright (c) 2017 INDONESIAN JOURNAL OF PHARMACY
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Indonesian J Pharm indexed by:
View My Stats